keyword
MENU ▼
Read by QxMD icon Read
search

Sphingomyelin

keyword
https://www.readbyqxmd.com/read/29155700/phase-behavior-of-charged-vesicles-under-symmetric-and-asymmetric-solution-conditions-monitored-with-fluorescence-microscopy
#1
Bastian Kubsch, Tom Robinson, Jan Steinkühler, Rumiana Dimova
Phase-separated giant unilamellar vesicles (GUVs) exhibiting coexisting liquid-ordered and liquid-disordered domains are a common biophysical tool to investigate the lipid raft hypothesis. Numerous studies, however, neglect the impact of physiological solution conditions. On that account, the current work presents the effect of high-salinity buffer and trans-membrane solution asymmetry on liquid-liquid phase separation in charged GUVs grown from dioleylphosphatidylglycerol, egg sphingomyelin, and cholesterol...
October 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29148800/size-and-shape-of-amyloid-fibrils-induced-by-ganglioside-nanoclusters-role-of-sialyl-oligosaccharide-in-fibril-formation
#2
Teruhiko Matsubara, Masaya Nishihara, Hanaki Yasumori, Mako Nakai, Katsuhiko Yanagisawa, Toshinori Sato
Ganglioside-enriched microdomains in the presynaptic neuronal membrane play a key role in the initiation of amyloid ß-protein (Aß) assembly related to Alzheimer's disease. We previously isolated lipids from a detergent-resistant membrane microdomain fraction of synaptosomes prepared from aged mouse brain and found that spherical Aß assemblies were formed on Aß-sensitive ganglioside nanoclusters (ASIGN) of reconstituted lipid bilayers in the synaptosomal fraction. In the present study, we investigated the role of oligosaccharides in Aß fibril formation induced by ganglioside-containing mixed lipid membranes that mimic the features of ASIGN...
November 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29142974/profiling-of-plasma-metabolites-suggests-altered-mitochondrial-fuel-usage-and-remodeling-of-sphingolipid-metabolism-in-individuals-with-type%C3%A2-2-diabetes-and-kidney-disease
#3
Jian-Jun Liu, Sujoy Ghosh, Jean-Paul Kovalik, Jianhong Ching, Hyung Won Choi, Subramaniam Tavintharan, Choon Nam Ong, Chee Fang Sum, Scott A Summers, E Shyong Tai, Su Chi Lim
Introduction: Pathophysiology of diabetic kidney disease (DKD) is incompletely understood. We aim to elucidate metabolic abnormalities associated with DKD in type 2 diabetes mellitus (T2DM) by targeted plasma metabolomics. Methods: A total of 126 T2DM participants with early DKD (urinary albumin-to-creatinine ratio [ACR] 30-299 mg/g and eGFR ≥ 60 ml/min/1.73 m(2)), 154 overt DKD (ACR ≥ 300 mg/g or eGFR < 60 ml/min/1.73 m(2)), and 129 non-DKD T2DM controls (ACR < 30 mg/g and eGFR ≥ 60 ml/min/1...
May 2017: KI Reports
https://www.readbyqxmd.com/read/29138414/potential-application-of-1-h-nmr-for-routine-serum-lipidome-analysis-evaluation-of-effects-of-bariatric-surgery
#4
Adriana Mika, Zbigniew Kaczynski, Piotr Stepnowski, Maciej Kaczor, Monika Proczko-Stepaniak, Lukasz Kaska, Tomasz Sledzinski
Routine laboratory lipid assays include simple measurements of total cholesterol, triacylglycerols and HDL. However, lipids are a large group of compounds involved in many metabolic pathways, and their alterations may have serious health consequences. In this study, we used (1)H NMR to analyze lipids extracted from sera of 16 obese patients prior to and after bariatric surgeries. We observed a post-surgery decrease in serum concentrations of lipids from various groups. The hereby presented findings imply that (1)H NMR is suitable for rapid, simple and non-invasive detection of lipids from 30 structural groups, among them triacylglycerols, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, total phospholipids, total, free and esterified cholesterol, total and unsaturated fatty acids...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29132110/comprehensive-phospholipid-and-sphingomyelin-profiling-of-different-brain-regions-in-mouse-model-of-anxiety-disorder-using-online-two-dimensional-hilic-rp-lc-ms-method
#5
Róbert Berkecz, Ferenc Tömösi, Tímea Körmöczi, Viktor Szegedi, János Horváth, Tamás Janáky
A novel online system including two-dimensional liquid chromatography coupled to high-resolution mass spectrometry (2D-LC/MS) was developed and applied for comprehensive phospholipid (PL) and sphingomyelin (SM) profiling of dorsal hippocampus (DHPC), ventral (VHPC) and prefrontal cortex (PFC) brain regions in a mouse model of anxiety disorder. In the first dimension, lipid classes were distinguished by hydrophilic interaction liquid chromatography (HILIC), while the second dimensional separation of individual PL and SM species was achieved by reversed-phase (RP) chromatography...
November 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29129753/nitrogen-mustard-induced-corneal-injury-involves-the-sphingomyelin-ceramide-pathway
#6
Georgia Charkoftaki, James Jester, David Thompson, Vasilis Vasiliou
PURPOSE: Nitrogen mustard (NM), which simulates the effects of sulfur mustard (SM), is a potent vesicant known to cause irreversible corneal damage. This study investigates the mechanisms by which NM induces corneal damage by examining the impact of NM exposure on the morphology and lipidome of the cornea. METHODS: Intact ex vivo rabbit eyes were placed in serum-free DMEM organ culture. NM (0, 1, 2.5, 5 or 10 mg/ml) was applied to the central cornea for 5, 10 or 15 min using a 5 mm filter disk and subsequently rinsed with DMEM...
November 9, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29128370/the-inhibition-of-sphingomyelin-synthase-1-activity-induces-collecting-duct-cells-to-lose-their-epithelial-phenotype
#7
Yamila Romina Brandán, Edith Del Valle Guaytima, Nicolás Octavio Favale, Lucila Gisele Pescio, Norma B Sterin-Speziale, María Gabriela Márquez
Epithelial tissue requires that cells attach to each other and to the extracellular matrix by the assembly of adherens junctions (AJ) and focal adhesions (FA) respectively. We have previously shown that, in renal papillary collecting duct (CD) cells, both AJ and FA are located in sphingomyelin (SM)-enriched plasma membrane microdomains. In the present work, we investigated the involvement of SM metabolism in the preservation of the epithelial cell phenotype and tissue organization. To this end, primary cultures of renal papillary CD cells were performed...
November 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29125130/acid-sphingomyelinase-inhibitors-imipramine-and-zoledronic-acid-increase-skeletal-muscle-tissue-sensitivity-to-insulin-action-at-old-age
#8
Loay Kh M Hassouneh, Olga A Timofiychuk, Nataliya A Babenko
Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old age. The 3-, 12- and 24-month-old Wistar male rats were used in the experiments. The progressive increase of ceramide content and ceramide/sphingomyeline (SM) ratio was determined in the extensor digitorum longus (EDL) muscle during aging of rats...
November 10, 2017: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29105220/correlative-mass-spectrometry-imaging-applying-tof-sims-and-ap-maldi-to-a-single-tissue-section
#9
N Desbenoit, A Walch, B Spengler, A Brunelle, A Römpp
RATIONALE: Mass spectrometry imaging (MSI) is a powerful tool for mapping the surface of a sample. Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization (AP-MALDI) offer complementary capabilities. Here, we present a workflow to apply both techniques to a single tissue section and combine the resulting data on the example of human colon cancer tissue. METHODS: Following cryo-sectioning, images were acquired using the high spatial resolution (1 μm pixel size) provided by TOF-SIMS...
November 3, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29103713/short-communication-circulating-fatty-acylcarnitines-are-elevated-in-overweight-periparturient-dairy-cows-in-association-with-sphingolipid-biomarkers-of-insulin-resistance
#10
J E Rico, Y Zang, N J Haughey, A G Rius, J W McFadden
Defects in mitochondrial fatty acid processing are associated with the development of fatty liver disease, inflammation, and insulin resistance in overweight nonruminants. Surplus fatty acids (FA) and defects in FA oxidation favor the accumulation of fatty acylcarnitines (FAC) and the sphingolipid ceramide. Moreover, elevated circulating FAC and ceramide concentrations are inversely related to insulin action. Because we have previously demonstrated that plasma ceramide levels increase during the transition from gestation to lactation, our aim was to determine whether changes in plasma medium- and long-chain FAC levels are related to circulating FA and sphingolipids in peripartal dairy cows...
November 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29101899/lipidomics-reveals-accumulation-of-the-oxidized-cholesterol-in-erythrocytes-of-heart-failure-patients
#11
Hsiang-Yu Tang, Chao-Hung Wang, Hung-Yao Ho, Pei-Ting Wu, Chun-Ling Hung, Cheng-Yu Huang, Pei-Ru Wu, Yung-Hsin Yeh, Mei-Ling Cheng
Lipids play an important role in the pathogenesis of cardiovascular disease. Changes in lipids of erythrocytes are indicative of the outcome of pathophysiological processes. In the present study, we assessed whether the lipid profiles of erythrocytes from heart failure (HF) patients are informative of their disease risk. The lipidomes of erythrocytes from 10 control subjects and 29 patients at different HF stages were analyzed using liquid chromatography time-of-flight mass spectrometry. The lipid composition of erythrocytes obtained from HF patients was significantly different from that of normal controls...
October 26, 2017: Redox Biology
https://www.readbyqxmd.com/read/29095713/bicuspid-aortic-valve-associated-aortopathy-update-on-biomarkers
#12
Ashna K Maredia, Steven C Greenway, Subodh Verma, Paul W M Fedak
PURPOSE OF REVIEW: Bicuspid aortic valve (BAV)-associated aortopathy is common and its progression for individual patients is difficult to predict. The present review aims to identify recent developments using biomarkers for the determination of risk and progression of disease in patients with BAV aortopathy. RECENT FINDINGS: Novel rare genetic variants and epigenetic methylation signatures affecting non-cytosine phosphate guanine (non-CpG) and CpG sites, nicotinamide phosphoribosyltransferase and Sod expression may lead to improved prediction of the aortopathy phenotype...
October 31, 2017: Current Opinion in Cardiology
https://www.readbyqxmd.com/read/29084218/concerted-regulation-of-npc2-binding-to-endosomal-lysosomal-membranes-by-bis-monoacylglycero-phosphate-and-sphingomyelin
#13
Giray Enkavi, Heikki Mikkolainen, Burçin Güngör, Elina Ikonen, Ilpo Vattulainen
Niemann-Pick Protein C2 (npc2) is a small soluble protein critical for cholesterol transport within and from the lysosome and the late endosome. Intriguingly, npc2-mediated cholesterol transport has been shown to be modulated by lipids, yet the molecular mechanism of npc2-membrane interactions has remained elusive. Here, based on an extensive set of atomistic simulations and free energy calculations, we clarify the mechanism and energetics of npc2-membrane binding and characterize the roles of physiologically relevant key lipids associated with the binding process...
October 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/29079454/the-adenosine-a2a-receptor-agonist-t1-11-ameliorates-neurovisceral-symptoms-and-extends-the-lifespan-of-a-mouse-model-of-niemann-pick-type-c-disease
#14
Antonella Ferrante, Antonella Pezzola, Andrea Matteucci, Antonella Di Biase, Lucilla Attorri, Monica Armida, Alberto Martire, Yijuang Chern, Patrizia Popoli
Niemann-Pick C is a fatal neurovisceral disorder caused, in 95% of cases, by mutation of NPC1 gene. Therapeutic options are extremely limited and new "druggable" targets are highly warranted. We previously demonstrated that the stimulation of the adenosine A2A receptor (A2AR) normalized the pathological phenotype of cellular models of NPC1. Since the validation of A2ARs as a therapeutic target for NPC1 can be obtained only conducting studies in in vivo models of the disease, in the present paper, the effects of two agonists of A2ARs were evaluated in the mouse model Balb/c Npc1(nih), hereafter indicated as NPC1-/-...
October 25, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/29076348/synthesis-and-evaluation-of-xanthone-derivatives-as-acid-sphingomyelinase-inhibitors-potential-treatment-for-uv-induced-skin-damage
#15
Kan Yang, Biao Gao, Xianjun Ming, Zhipeng Huang, Minghui Wang, Jibin Dong, Jinxin Wang
AIM: ASM, which hydrolyzes sphingomyelin into ceramide, is recognized as a therapeutic target for UV-induced skin damage. Direct inhibitors for this enzyme are rare. Here we synthesized several series of 1,3,6,7-tetrahydroxy-xanthone derivatives as novel ASM inhibitors. RESULTS: Several compounds were more potent than the lead compound, among which 5b was found competitively inhibiting the enzyme and dose-dependently reducing ceramide generation. Furthermore, 5b and 5c showed excellent protective effect to skin keratinocytes against UV...
October 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/29072338/determination-of-lipid-bilayer-affinities-and-solvation-characteristics-by-electrokinetic-chromatography-using-polymer-bound-lipid-bilayer-nanodiscs
#16
William M Penny, Christopher P Palmer
Styrene-maleic acid polymer-bound lipid bilayer nanodiscs have been investigated and characterized by electrokinetic chromatography. Linear solvation energy relationship analysis was employed to characterize the changes in solvation environment of nanodiscs of varied belt to lipid ratio, belt polymer chemistry and molecular weight, and lipid composition. Increases in the lipid to belt polymer ratio resulted in smaller, more cohesive nanodiscs with greater electrophoretic mobility. Nanodisc structures with belt polymers of different chemistry and molecular weight were compared and showed only minor changes in solvent characteristics and selectivity consistent with changes in structure of the lipid bilayer...
October 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/29070689/both-sphingomyelin-and-cholesterol-in-the-host-cell-membrane-are-essential-for-rubella-virus-entry
#17
Noriyuki Otsuki, Masafumi Sakata, Kyoko Saito, Kiyoko Okamoto, Yoshio Mori, Kentaro Hanada, Makoto Takeda
Rubella virus (RuV) causes a systemic infection and transplacental fetal infection causes congenital rubella syndrome. In this study, we showed that treatment of cells with sphingomyelinase inhibited RuV infection. Assays using inhibitors of serine palmitoyl transferase and ceramide transport protein demonstrated the contribution of sphingomyelin (SM) to RuV infection. Compelling evidence for direct binding of RuV to lipid membranes at neutral pH was obtained using liposome co-flotation assays. The absence of either SM or cholesterol (Chol) abrogated the RuV--liposome interaction...
October 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29064256/long-term-stability-of-human-plasma-metabolites-during-storage-at-80-%C3%A2-c
#18
Mark Haid, Caroline Muschet, Simone Wahl, Werner Römisch-Margl, Cornelia Prehn, Gabriele Möller, Jerzy Adamski
Prolonged storage of biospecimen can lead to artificially altered metabolite concentrations and thus bias data analysis in metabolomics experiments. To elucidate the potential impact of long-term storage on the metabolite profile, a pooled human plasma sample was aliquoted and stored at 80 °C. During a time period of five years, 1012 of the aliquots were measured with the Biocrates AbsoluteIDQ® p180 targeted-metabolomics assay at 193 time points. Modelling the concentration courses over time revealed that 55 out of 111 metabolites remained stable...
October 24, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29063180/serum-lipid-feature-and-potential-biomarkers-of-lethal-ventricular-tachyarrhythmia-lvta-induced-by-myocardial-ion-channel-diseases-a-rat-model-study
#19
Jiayan Wu, Qian Wu, WenTao Dai, Jing Kong, Junyao Lv, Xiaojun Yu, Xingxing Wang, Dian Wang
To determine the cause of death in myocardial ion channel diseases (MICD)-induced sudden cardiac death (SCD) cases is a difficulty in forensic identification practices. The majority of MICD-induced SCD cases would experience lethal ventricular tachyarrhythmia (LVTA) before deaths; thus, confirming the occurrence of LVTA in bodies can offer a key evidence to identify these cases. Several lipids in the myocardia were found disturbed after LVTA; yet, whether serum lipidome would be disrupted by LVTA is not clear...
October 23, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29053806/independent-association-of-plasma-hydroxysphingomyelins-with-physical-function-in-the-atherosclerosis-risk-in-communities-aric-study
#20
Danni Li, Jeffrey R Misialek, Fangying Huang, B Gwen Windham, Fang Yu, Alvaro Alonso
Background: Plasma metabolites such as phosphatidylcholines (PCs) and sphingomyelins (SMs) are associated with in age-related cognitive decline. However, their relations to age-related physical function decline remain largely unknown. Methods: We examined the cross-sectional relations of 12 plasma metabolites (including 4 PCs and 4 SMs) with physical function in 383 older adults in the Atherosclerosis Risk in Communities study at the fifth exam (2011-13, mean age [standard deviation (SD)]: 78...
October 19, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
keyword
keyword
44742
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"