keyword
MENU ▼
Read by QxMD icon Read
search

Beclin1

keyword
https://www.readbyqxmd.com/read/27916732/ghrelin-improves-vascular-autophagy-in-rats-with-vascular-calcification
#1
Mingming Xu, Lin Liu, Chenfang Song, Wei Chen, Shuyan Gui
BACKGROUNDS: This study aimed to investigate whether ghrelin ameliorated vascular calcification (VC) through improving autophagy. METHODS: VC model was induced by nicotine plus vitamin D3 in rats and β-glycerophosphate in vascular smooth muscle cell (VSMC). Calcium deposition was detected by von Kossa staining or alizarin red S staining. ALP activity was also detected. Western blot was used to assess the protein expression. RESULTS: Ghrelin treatment attenuated the elevation of calcium deposition and ALP activity in VC model both in vivo and in vitro...
December 1, 2016: Life Sciences
https://www.readbyqxmd.com/read/27904666/ad-hgf-improves-the-cardiac-remodeling-of-rat-following-myocardial-infarction-by-upregulating-autophagy-and-necroptosis-and-inhibiting-apoptosis
#2
Jiabao Liu, Peng Wu, Yunle Wang, Yingqiang Du, Nan A, Shuiyuan Liu, Yiming Zhang, Ningtian Zhou, Zhihui Xu, Zhijian Yang
Cell death in MI is the most critical determinant of subsequent left ventricular remodeling and heart failure. Besides apoptosis, autophagy and necroptosis have been recently found to be another two regulated cell death styles. HGF has been reported to have a protective role in MI, but its impact on the three death styles remains unclear. Thus, our study was performed to investigate the distribution of autophagy, apoptosis and necroptosis in cardiac tissues after MI and explore the role and mechanism of Ad-HGF on cardiac remodeling by regulating the three death styles...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27903966/pde5-inhibitors-enhance-the-lethality-of-pemetrexed-through-inhibition-of-multiple-chaperone-proteins-and-via-the-actions-of-cyclic-gmp-and-nitric-oxide
#3
Laurence Booth, Jane L Roberts, Andrew Poklepovic, Sarah Gordon, Paul Dent
Phosphodiesterase 5 (PDE5) inhibitors prevent the breakdown of cGMP that results in prolonged protein kinase G activation and the generation of nitric oxide. PDE5 inhibitors enhanced the anti-NSCLC cell effects of the NSCLC therapeutic pemetrexed. [Pemetrexed + sildenafil] activated an eIF2α - ATF4 - CHOP - Beclin1 pathway causing formation of toxic autophagosomes; activated a protective IRE1 - XBP-1 - chaperone induction pathway; and activated a toxic eIF2α - CHOP - DR4 / DR5 / CD95 induction pathway. [Pemetrexed + sildenafil] reduced the expression of c-FLIP-s, MCL-1 and BCL-XL that was blocked in a cell-type -dependent fashion by either over-expression of HSP90 / GRP78 / HSP70 / HSP27 or by blockade of eIF2α-CHOP signaling...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27895792/microrna-199a-5p-inhibits-cisplatin-induced-drug-resistance-via-inhibition-of-autophagy-in-osteosarcoma-cells
#4
Yusheng Li, Wei Jiang, Yuling Hu, Zixun Da, Chao Zeng, Min Tu, Zhenhan Deng, Wenfeng Xiao
Osteosarcoma (OS) is the most common cancer of the bone. Chemotherapy is commonly used for the clinical treatment of OS. However, chemoresistance to cisplatin [also known as diamminedichloridoplatinum (II) (DDP)] is a major obstacle for OS therapy, the underlying mechanism of which is not fully understood. The present study aimed to investigate the role of microRNA (miR)-199a-5p in the regulation of chemoresistance to DDP in OS cells. Reverse transcription-quantitative polymerase chain reaction demonstrated that the expression level of miR-199a-5p was significantly reduced in human OS MG63 cells...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27882565/tanshinone-iia-attenuates-a%C3%AE-25-35-induced-spatial-memory-impairment-via-upregulating-receptors-for-activated-c-kinase1-and-inhibiting-autophagy-in-hippocampus
#5
Jiejun Zhu, Shiping Liao, Liming Zhou, Lihong Wan
OBJECTIVES: Tanshinone IIA (Tan IIA) may exert significant protective effects against the neurotoxicity induced by β-amyloid protein (Aβ). This study was designed to investigate the possible neuroprotective mechanism of Tan IIA on Aβ25-35 -induced spatial memory impairment in mice. METHODS: After 3 weeks of preventive treatment (Tan IIA or oil), all male Kunming mice were subjected to Aβ25-35 (10 μl, intracerebroventricularly (i.c.v.)) to establish the spatial memory impairment model...
November 23, 2016: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/27877136/novel-role-of-er-stress-and-autophagy-in-microcystin-lr-induced-apoptosis-in-chinese-hamster-ovary-cells
#6
Shenshen Zhang, Chuanrui Liu, Yang Li, Mustapha U Imam, Hui Huang, Haohao Liu, Yongjuan Xin, Huizhen Zhang
Microcystin-LR (MC-LR) is a ubiquitous peptide that exhibits strong reproductive toxicity, although the mechanistic basis for such toxicity remains largely unknown. The present study was conducted to investigate the mechanisms underlying the adverse effects of exposure to MC-LR in Chinese hamster ovary (CHO) cells. The results showed that MC-LR inhibited the in vitro proliferation of CHO cells significantly, with an IC50 of 10 μM. Moreover, MC-LR-treated CHO cells revealed strong induction of cell cycle arrest and apoptosis...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27875637/ambra1-a-novel-%C3%AE-synuclein-binding-protein-is-implicated-in-the-pathogenesis-of-multiple-system-atrophy
#7
Yasuo Miki, Kunikazu Tanji, Fumiaki Mori, Yota Tatara, Jun Utsumi, Hidenao Sasaki, Akiyoshi Kakita, Hitoshi Takahashi, Gian Maria Fimia, Koichi Wakabayashi
The accumulation of abnormal α-synuclein is the major histopathological feature of Lewy body disease and multiple system atrophy (MSA), which are referred to as synucleinopathies. Although the pathomechanisms underlying synucleinopathies are still unknown, mounting evidence indicates that cytoplasmic degradation systems, such as the autophagy-lysosome and proteasome pathways, are strongly involved in their pathogenesis. Autophagy is tightly regulated by several upstream proteins including UNC-51-like kinase 1/2, beclin1, vacuolar protein sorting-associated protein 34, and autophagy/beclin1 regulator 1 (AMBRA1)...
November 22, 2016: Brain Pathology
https://www.readbyqxmd.com/read/27875278/ratiometric-analysis-of-acridine-orange-staining-in-the-study-of-acidic-organelles-and-autophagy
#8
Marcos Paulo Thomé, Eduardo Cremonese Filippi-Chiela, Emilly Schlee Villodre, Celina Borges Migliavaca, Giovana Ravizzoni Onzi, Karina Bettega Felipe, Guido Lenz
Acridine orange (AO) is a cell permeable green fluorophore that can be protonated and trapped in Acidic Vesicular Organelles (AVOs). Its metachromatic shift to red fluorescence is concentration-dependent and, therefore, AO fluoresces red in AVOs, such as autolysosomes. This makes AO staining a quick, accessible and reliable method to assess the volume of AVOs, which increases upon autophagy induction. Here we describe a ratiometric analysis of autophagy using AO, considering the red-to-green fluorescence intensity ratio (R/GFIR) to quantify flow cytometry and fluorescence microscopy data of AO-stained cells...
November 14, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27872163/role-of-autophagy-in-cadmium-induced-testicular-injury
#9
Y-J Wang, J Yan, F Yin, L Li, Y-G Qin, C-Y Meng, R-F Lu, L Guo
The testis is sensitive to cadmium, but studies investigating cadmium-induced testicular injury have not yet clearly revealed the underlying mechanisms. This study aimed to investigate the injurious effects of cadmium on rat testes and the role that autophagy plays in this process. Wistar rats were randomly divided into four groups and intraperitoneally injected with 0.2 (low), 0.4 (middle), and 0.8 mg/kg·body weight (high) cadmium chloride for 5 weeks, while the control rats were injected with equal volume of saline...
November 21, 2016: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/27853422/rock1-is-associated-with-alzheimer-s-disease-specific-plaques-as-well-as-enhances-autophagosome-formation-but-not-autophagic-a%C3%AE-clearance
#10
Yong-Bo Hu, Yang Zou, Yue Huang, Yong-Fang Zhang, Guinevere F Lourenco, Sheng-Di Chen, Glenda M Halliday, Gang Wang, Ru-Jing Ren
Alzheimer's disease (AD) is the most prevalent form of late-life dementia in the population, characterized by amyloid plaque formation and increased tau deposition, which is modulated by Rho-associated coiled-coil kinase 1 (ROCK1). In this study, we further analyze whether ROCK1 regulates the metabolism of amyloid precursor protein (APP). We show that ROCK1 is colocalized with mature amyloid-β (Aβ) plaques in patients with AD, in that ROCK1 enhances the amyloidogenic pathway, and that ROCK1 mediated autophagy enhances the intracellular buildup of Aβ in a cell model of AD (confirmed by increased ROCK1 and decreased Beclin 1 protein levels, with neuronal autophagosome accumulation in prefrontal cortex of AD APP/PS1 mouse model)...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27831944/receptor-for-advanced-glycation-end-product-blockade-enhances-the-chemotherapeutic-effect-of-cisplatin-in-tongue-squamous-cell-carcinoma-by-reducing-autophagy-and-modulating-the-wnt-pathway
#11
Ziming Zhao, Hongyu Wang, Liao Zhang, Xifan Mei, Jing Hu, Keqiang Huang
Tongue squamous cell carcinoma (TSCC) is one of the most severe types of cancer with poor outcomes. Cisplatin is used widely to treat cancer cells, but many patients develop acquired drug resistance. The receptor for advanced glycation end products (RAGE) is expressed widely in TSCC and associated with drug-induced chemotherapy resistance. However, the effect of RAGE and cisplatin on Tca-8113 cells remains unknown. We assayed the combined use of RAGE blockade and cisplatin effect on Tca-8113 cells' viability by MTT and apoptosis rate of Tca-8113 cells on RAGE blockade+cisplatin treatment; cisplatin alone; or RAGE blockade alone by flow cytometry...
November 9, 2016: Anti-cancer Drugs
https://www.readbyqxmd.com/read/27825801/targeting-autophagy-augments-the-activity-of-dha-e3-to-overcome-p-gp-mediated-multi-drug-resistance
#12
Guangmin Xi, Ming Wang, Bing Sun, Abdul Sami Shaikh, Yongqing Liu, Wei Wang, Hongxiang Lou, Huiqing Yuan
Multidrug resistance (MDR) is a major obstacle for successful chemotherapy treatment. Searching for effective MDR modulators and combining them with anticancer drug therapies has been a promising strategy against clinical MDR. In our previous study, we have found that DHA-E3, a synthetic derivative of DHA, has the ability to modulate the function of P-glycoprotein (P-gp) and reverse MDR in cancer cells. In this study, we further evaluated the reversal effect of DHA-E3 on MDR and explored its mechanism of action in vitro...
November 5, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27825566/defective-autophagy-activity-and-its-association-with-spinal-damage-in-patients-with-ankylosing-spondylitis
#13
Min-Chan Park, Hye Won Kim, Sang-Won Lee, Jason Jungsik Song, Yong-Beom Park
OBJECTIVES: This study was performed to determine the expression levels of autophagy-related genes in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and to investigate their associations with clinical parameters reflecting disease activity and radiographic progression and with cytokine levels of AS patients. METHODS: PBMCs from 53 AS patients and 49 healthy controls were obtained and mRNA expression levels of LC3, belcin1, and ATG5 were determined using quantitative real-time PCR...
November 4, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/27818354/prolyl-oligopeptidase-inhibition-attenuates-the-toxicity-of-a-proteasomal-inhibitor-lactacystin-in-the-alpha-synuclein-overexpressing-cell-culture
#14
Timo T Myöhänen, Susanna Norrbacka, Mari H Savolainen
Lewy bodies, the histopathological hallmarks of Parkinson's disease (PD), contain insoluble and aggregated α-synuclein (aSyn) and many other proteins, proposing a role for failure in protein degradation system in the PD pathogenesis. Proteasomal dysfunction has indeed been linked to PD and aSyn oligomers have been shown to inhibit proteasomes and autophagy. Our recent studies have shown that inhibitors of prolyl oligopeptidase (PREP) can prevent the aggregation and enhance the clearance of accumulated aSyn, and therefore, we wanted to study if PREP inhibition can overcome the aSyn aggregation and toxicity induced by lactacystin, a proteasomal inhibitor...
November 3, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/27817185/pharmacologic-inhibition-of-autophagy-sensitizes-human-acute-leukemia-jurkat-t-cells-to-acacetin-induced-apoptosis
#15
Ji Young Lee, Do Youn Jun, Ki Yun Kim, Eun Ji Ha, Mi Hee Woo, Jee Youn Ko, Young Ho Yun, In-Seok Oh, Young Ho Kim
Exposure of Jurkat T cell clone (J/Neo cells) to acacetin (5,7-dihydroxy-4'-methoxyflavone), which is present in barnyard millet [Echinochloa esculenta (A. Braun)] grains, resulted in cytotoxicity, accumulation of apoptotic sub-G1 cells, Bak activation, loss of mitochondrial membrane potential (Δψm), activation of caspase-9 and caspase-3, cleavage of poly (ADP-ribose) polymerase (PARP), and FITC-Annexin V-stainable phosphatidylserine exposure on the external surface of the cytoplasmic membrane without accompanying necrosis...
November 4, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27806347/autophagy-negatively-regulates-tumor-cell-proliferation-through-phosphorylation-dependent-degradation-of-the-notch1-intracellular-domain
#16
Ji-Seon Ahn, Eun-Jung Ann, Mi-Yeon Kim, Ji-Hye Yoon, Hye-Jin Lee, Eun-Hye Jo, Keesook Lee, Ji Shin Lee, Hee-Sae Park
Autophagy is a highly conserved mechanism that degrades long-lived proteins and dysfunctional organelles, and contributes to cell fate. In this study, autophagy attenuates Notch1 signaling by degrading the Notch1 intracellular domain (Notch1-IC). Nutrient-deprivation promotes Notch1-IC phosphorylation by MEKK1 and phosphorylated Notch1-IC is recognized by Fbw7 E3 ligase. The ubiquitination of Notch1-IC by Fbw7 is essential for the interaction between Notch1-IC and p62 and for the formation of aggregates. Inhibition of Notch1 signaling prevents the transformation of breast cancer cells, tumor progression, and metastasis...
October 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27782356/simvastatin-alleviates-airway-inflammation-and-remodelling-through-up-regulation-of-autophagy-in-mouse-models-of-asthma
#17
Wen Gu, Rong Cui, Tao Ding, Xiaoming Li, Juan Peng, Weiguo Xu, Fengfeng Han, Xuejun Guo
BACKGROUND AND OBJECTIVE: Statins have been widely used in inflammatory diseases including asthma, because of their anti-inflammatory and immunomodulatory properties. It has been shown that simvastatin induces autophagy and cell death in some circumstances. However, the possible cross-talk between simvastatin and autophagic processes in lung disease is largely unknown. Thus, we investigated the impact of simvastatin on airway inflammation and airway remodelling and the possible relationship of these processes to a simvastatin-induced autophagic pathway in mouse models of asthma...
October 26, 2016: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/27780926/resistance-of-glioma-cells-to-nutrient-deprived-microenvironment-can-be-enhanced-by-cd133-mediated-autophagy
#18
Haojie Sun, Mingzhi Zhang, Kai Cheng, Peng Li, Shuo Han, Ruizhi Li, Ming Su, Wotan Zeng, Jinwen Liu, Jinhai Guo, Yinan Liu, Xiaoyan Zhang, Qihua He, Li Shen
CD133 is a pentaspan transmembrane protein that can serve as a biomarker for cancer stem cells, although its biochemical mechanism remains unclear. Here we report that CD133 expression enhances glioma cell tolerance of a nutrient-deprived microenvironment. Under starvation conditions, CD133-positive cells exhibited higher survival and decreased levels of apoptosis. These changes were dependent on activation of autophagy-associated gene signaling and were impaired by the autophagic inhibitor chloroquine. Furthermore, rapamycin up-regulated the level of autophagy and inversely reduced CD133 expression...
October 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/27774947/-human-papillomavirus-16-e5-protein-inhibits-keratinocyte-growth-factor-triggered-autophagy
#19
Guanghong Fu, Dan Gong, Jia Wan
Objective To investigate the effect of E5 protein of human papillomavirus type 16 (HPV16 E5) on keratinocyte growth factor-triggered autophagy. Methods Firstly, pCI-neo-E5 recombinant plasmid was constructed. After that, the recombinant plasmid harboring pCI-neo-E5 was transfected into HaCaT cells using Lipofectamine(TM)2000 reagent, and the mRNA transcriptional level of 16 E5 was evaluated by quantitative real-time PCR (qRT-PCR). Then, the expression of LC3BIIwas detected by Western blotting and the localization of LC3B was observed by immunofluorescence in pCI-neo-E5-HA-transfected HaCaT cells triggered with keratinocyte growth factor...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27770734/downregulated-endogenous-sulfur-dioxide-aspartate-aminotransferase-pathway-is-involved-in-angiotensin-ii-stimulated-cardiomyocyte-autophagy-and-myocardial-hypertrophy-in-mice
#20
Qinghua Chen, Lulu Zhang, Siyao Chen, Yaqian Huang, Kun Li, Xiaoqi Yu, Huijuan Wu, Xiaoyu Tian, Chunyu Zhang, Chaoshu Tang, Junbao Du, Hongfang Jin
BACKGROUND: The study was designed to investigate if endogenous sulfur dioxide (SO2) was involved in cardiomyocyte autophagy and myocardial hypertrophy stimulated by angiotensin II (Ang II). METHODS: Thirty-two C57 mice were randomly divided into control, SO2, Ang II and Ang II+SO2 groups. Human myocardial cell line H9c2 was divided into four groups including control, SO2, Ang II and Ang II+SO2 groups. Blood pressure and myocardial hypertrophy of the mice were measured two weeks after Ang II administration...
September 30, 2016: International Journal of Cardiology
keyword
keyword
44686
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"