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https://www.readbyqxmd.com/read/29050003/cantharidin-inhibits-the-growth-of-triple-negative-breast-cancer-cells-by-suppressing-autophagy-and-inducing-apoptosis-in-vitro-and-in-vivo
#1
Hong-Chang Li, Zhi-Hua Xia, Ya-Feng Chen, Fan Yang, Wen Feng, Han Cai, Yi Mei, Yi-Ming Jiang, Ke Xu, Dian-Xu Feng
BACKGROUND/AIMS: Cantharidin, a type of terpenoid secreted by the blister beetle Mylabris phalerata (Pallas), has attracted great attention in cancer therapy because of its potential anti-cancer activities. Here, we report the effects on apoptosis and autophagy in human triple-negative breast cancer (TNBC) cell lines after treatment with cantharidin and attempt to elucidate the underlying mechanisms. METHODS: MDA-MB-231 and MDA-MB-468 cells were treated with cantharidin and cell proliferation was examined using CCK-8 and clone formation assays...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29035754/regulation-of-autophagy-factors-by-oxidative-stress-and-cardiac-enzymes-imbalance-during-arsenic-or-and-copper-induced-cardiotoxicity-in-gallus-gallus
#2
Siwen Li, Hongjing Zhao, Yu Wang, Yizhi Shao, Bangyi Wang, Yulong Wang, Mingwei Xing
Basal autophagy has an indispensable role in the functioning and maintenance of cardiac geometry under physiological conditions. Recently, increasing evidence has demonstrated that arsenic (As)/copper (Cu) play important roles in the autophagy of the heart. The current study was to evaluate whether oxidative damage by As or/and Cu was correlated with autophagy through the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway in the heart of birds. Arsenic trioxide (30mg/kg) or/and cupric sulfate (300mg/kg) were administered in a basal diet to male Hy-line chickens (one-day-old) for 12 weeks...
October 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29026190/growth-hormone-secretagogues-hexarelin-and-jmv2894-protect-skeletal-muscle-from-mitochondrial-damages-in-a-rat-model-of-cisplatin-induced-cachexia
#3
Giuseppe Sirago, Elena Conte, Flavio Fracasso, Antonella Cormio, Jean-Alain Fehrentz, Jean Martinez, Clara Musicco, Giulia Maria Camerino, Adriano Fonzino, Laura Rizzi, Antonio Torsello, Angela Maria Serena Lezza, Antonella Liantonio, Palmiro Cantatore, Vito Pesce
Chemotherapy can cause cachexia, which consists of weight loss associated with muscle atrophy. The exact mechanisms underlying this skeletal muscle toxicity are largely unknown and co-therapies to attenuate chemotherapy-induced side effects are lacking. By using a rat model of cisplatin-induced cachexia, we here characterized the mitochondrial homeostasis in tibialis anterior cachectic muscle and evaluated the potential beneficial effects of the growth hormone secretagogues (GHS) hexarelin and JMV2894 in this setting...
October 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29025057/nqo2-inhibition-relieves-ros-effects-on-mouse-oocyte-meiotic-maturation-and-embryo-development
#4
Dandan Chen, Xin Li, Xiaoyun Liu, Xiaoyu Liu, Xiuying Jiang, Juan Du, Qian Wang, Yuanjing Liang, Wei Ma
NRH: quinone oxidoreductase 2 (NQO2) is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone to hydroquinones. Herein, we assessed the protein expression, subcellular localization and possible functions of NQO2 in mouse oocyte meiotic maturation and embryo development. Western blot analysis detected high and stable protein expression of NQO2 in mouse oocytes during meiotic progression. Immunofluorescence illustrated NQO2 distribution on nuclear membrane, chromosomes and meiotic spindles...
September 4, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29023824/cholangiocyte-autophagy-contributes-to-hepatic-cystogenesis-in-polycystic-liver-disease-and-represents-a-potential-therapeutic-target
#5
Anatoliy I Masyuk, Tatyana V Masyuk, Maria J Lorenzo Pisarello, Jingyi Francess Ding, Lorena Loarca, Bing Q Huang, Nicholas F LaRusso
Polycystic liver disease (PLD) is a group of genetic disorders with limited treatment and significant morbidity. Hepatic cysts arise from cholangiocytes exhibiting a hyperproliferative phenotype. Considering that hyperproliferation of many cell types is associated with alterations in autophagy, we hypothesized that autophagy is altered in PLD cholangiocytes, contributes to hepatic cystogenesis, and might represent a potential therapeutic target. We employed Functional Pathway Cluster Analysis (FPCA) and NextGen Sequencing (NGS), transmission electron microscopy, immunofluorescence confocal microscopy, and western blotting to assess autophagy in human and rodent PLD cholangiocytes...
October 10, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29019932/asic1a-promotes-acid-induced-autophagy-in-rat-articular-chondrocytes-through-the-ampk-foxo3a-pathway
#6
Beibei Dai, Fei Zhu, Yong Chen, Renpeng Zhou, Zhisen Wang, Yaya Xie, Xiaoshan Wu, Shengqin Zu, Ge Li, Jinfang Ge, Feihu Chen
Acid-sensing ion channel 1a (ASIC1a) is a member of the extracellular H⁺-activated cation channels family. Our previous studies suggested that ASIC1a contributed to acid-induced rat articular chondrocytes autophagy. However, its potential mechanisms remain unclear. The present study demonstrated the effect of ASIC1a on rat articular chondrocytes autophagy and explored the underlying molecular mechanisms. The results demonstrated that ASIC1a contributed to acid-induced autophagy in rat articular chondrocytes, and which was associated with an increase in (Ca(2+))i, as indicated that acid-induced increases in mRNA and protein expression of LC3B-II and other autophagy-related markers were inhibited by ASIC1a-specific blocker, PcTx1 and calcium chelating agent, BAPTA-AM...
October 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29017169/ox-ldl-induced-microrna-155-promotes-autophagy-in-human-endothelial-cells-via-repressing-the-rheb-mtor-pathway
#7
Jinlin Lv, Lixia Yang, Ruiwei Guo, Yankun Shi, Ziwei Zhang, Jinshan Ye
BACKGROUND/AIMS: Autophagy, an evolutionary conserved biological process, is activated in cells to cope with various types of stress. MicroRNAs control several activities related to autophagy. However, the role of autophagy-related microRNAs during atherosclerosis is far from known. MicroRNA-155 was identified to be a crucial regulator of atherosclerosis. The objectives of the study were to analyze the effect of microRNA-155 on autophagic signaling and explore its mechanism in human endothelial cells under ox-LDL stress...
October 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28994040/the-protective-effect-of-selenium-on-the-chicken-pancreas-against-cadmium-toxicity-via-alleviating-oxidative-stress-and-autophagy
#8
Ruohan Liu, Tiantian Jia, Yuan Cui, Hongjin Lin, Shu Li
Cadmium (Cd) is a highly toxic heavy metal that can affect human and animal health. Selenium (Se) is an essential microelement that can protect various organs against toxic heavy metals. Although many studies have investigated the adverse effect of Cd in rats and several other animals, little is known regarding the mechanisms of Cd-induced autophagy in the chicken pancreas and the antagonistic effect of Se on Cd. In the current study, we fed chickens Se, Cd, or Se and Cd supplements to establish the Se and Cd interaction model and to measure the concentrations of Se and Cd in the chicken pancreas...
October 9, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28987828/acute-response-of-neurons-an-early-event-of-neuronal-cell-death-after-facial-nerve-injury
#9
Haitao Huang, Zhigang Sun, Huawei Liu, Juntao Ma, Min Hu
OBJECTIVE: This study was to research the early acute response events of facial nerve injury. METHODS: A total of 60 male Sprague-Dawley rats were randomly divided into two groups. For rats in study group, facial nerve anastomosis was performed. Rats in control group were undergone the same surgical procedure, but without cutting off the facial nerve. Before nerve anastomosis and at day 1, 3, 7, 14 and 28 after nerve anastomosis, 5 rats of each group were selected and right side brainstem tissue samples containing the facial nerve nucleus were obtained...
October 4, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28977849/endoplasmic-reticulum-stress-induces-autophagy-and-apoptosis-while-inhibiting-proliferation-and-drug-resistance-in-multiple-myeloma-through-the-pi3k-akt-mtor-signaling-pathway
#10
Yun-Feng Fu, Xiao Liu, Meng Gao, Ya-Nan Zhang, Jing Liu
We investigated the effects of endoplasmic reticulum stress (ERS) on autophagy, proliferation, apoptosis, and drug resistance in multiple myeloma (MM). MM patients enrolled in our study (n = 268) were classified into sensitive and resistant groups based on chemotherapy efficacy, and their serum levels of β2-MG, albumin (ALB), lactic dehydrogenase (LDH), Ca(2+) and hemoglobin were determined. In addition, human MM U266 and MOLP-2/R cells were divided into blank, tunicamycin (TM), TM + insulin-like growth factor-1 (IGF-1), and TM + rapamycin groups, and measured expression of ERS-related, PI3K/Akt/mTOR pathway-related, and autophagy-related mRNA and proteins...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28962872/ucp2-inhibition-induces-ros-akt-mtor-axis-role-of-gapdh-nuclear-translocation-in-genipin-everolimus-anticancer-synergism
#11
Ilaria Dando, Raffaella Pacchiana, Elisa Dalla Pozza, Ivana Cataldo, Stefano Bruno, Paola Conti, Marco Cordani, Anna Grimaldi, Giovanna Butera, Michele Caraglia, Aldo Scarpa, Marta Palmieri, Massimo Donadelli
Several studies indicate that mitochondrial uncoupling protein 2 (UCP2) plays a pivotal role in cancer development by decreasing reactive oxygen species (ROS) produced by mitochondrial metabolism and by sustaining chemoresistance to a plethora of anticancer drugs. Here, we demonstrate that inhibition of UCP2 triggers Akt/mTOR pathway in a ROS-dependent mechanism in pancreatic adenocarcinoma cells. This event reduces the antiproliferative outcome of UCP2 inhibition by genipin, creating the conditions for the synergistic counteraction of cancer cell growth with the mTOR inhibitor everolimus...
September 27, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28962169/microrna-320-regulates-autophagy-in-retinoblastoma-by-targeting-hypoxia-inducible-factor-1%C3%AE
#12
Yong Liang, Xi Chen, Zhu Liang
Retinoblastoma (RB) is the most common malignancy in children. Due to refractory mechanisms of chemoresistance and the toxicity of chemotherapies, novel therapies for RB treatment are urgently required. MicroRNA-320 (miR-320) is believed to be associated with the tumorigenesis of RB, although the mechanism remains unclear. Considering the hypoxic intratumoral region, the roles of miR-320 and hypoxia inducible factor-1α (HIF-1α) in the regulation of autophagy were investigated in 30 human RB samples and WERI-RB1 cells...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28944928/hydrogen-inhibits-isoproterenol%C3%A2-induced-autophagy-in-cardiomyocytes-in%C3%A2-vitro-and-in%C3%A2-vivo
#13
Yaxing Zhang, Zhiyuan Long, Jingting Xu, Sihua Tan, Nan Zhang, Anfei Li, Ling Wang, Tinghuai Wang
A previous study from our group has demonstrated that hydrogen administration can attenuate cardiovascular hypertrophy in vivo by targeting reactive oxygen species‑dependent mitogen‑activated protein kinase signaling. The aim of the present study is to determine the effect of hydrogen on cardiomyocyte autophagy during β‑adrenoceptor activation in vivo and in vitro. We prepared hydrogen‑rich medium, and the concentration of hydrogen was measured by using the MB‑Pt reagent method. For the in vitro study, H9c2 cardiomyocytes were stimulated with isoproterenol (ISO; 10 µM) for 5, 15 and 30 min, and then the protein expression levels of the autophagy marker microtubule‑associated protein 1 light chain 3β II (LC3B II) were examined by western blotting...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28928217/peptides-derived-from-evolutionarily-conserved-domains-in-beclin1-and-beclin2-enhance-the-entry-of-lentiviral-vectors-into-human-cells
#14
Saliha Majdoul, Jérémie Cosette, Ababacar K Seye, Eric Bernard, Sophie Frin, Nathalie Holic, Nathalie Chazal, Laurence Briant, Lucile Espert, Anne Galy, David Fenard
Autophagy-related proteins such as Beclin1 are involved in an array of complex processes including antiviral responses, and may also modulate the efficiency of gene therapy viral vectors. The TatBeclin1 (TB1) peptide has been reported as an autophagy-inducing factor inhibiting the replication of pathogens such as HIV-1. Yet, autophagy-related proteins are also essential for the early steps of HIV-1 infection. Therefore, we examined here the effects of the Beclin1-evolutionarily conserved domain in TB1 on viral transduction and autophagy in single-round HIV infection or with nonreplicative HIV-1-derived lentiviral vectors (LV)...
September 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28927274/fluoride-induced-autophagy-via-the-regulation-of-phosphorylation-of-mammalian-targets-of-rapamycin-in-mice-leydig-cells
#15
Jianhai Zhang, Yuchen Zhu, Yan Shi, Yongli Han, Chen Liang, Zhiyuan Feng, Heping Zheng, Michelle Eng, Jundong Wang
Fluoride is known to impair testicular function and decrease testosterone levels, yet the underlying mechanisms remain inconclusive. The objective of this study is to investigate the roles of autophagy in fluoride-induced male reproductive toxicity using both in vivo and in vitro Leydig cell models. Using transmission electron microscopy and monodansylcadaverine staining, we observed increasing numbers of autophagosomes in testicular tissue, especially in Leydig cells of fluoride-exposed mice. Further study revealed that fluoride increased the levels of mRNA and protein expression of autophagy markers LC3, Beclin1, and Atg 5 in primary Leydig cells...
October 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28904192/the-role-of-herpes-simplex-virus-type-1-%C3%AE-34-5-in-the-regulation-of-irf3-signaling
#16
Richard Manivanh, Jesse Mehrbach, David M Knipe, David A Leib
During viral infection, pattern recognition receptors (PRRs) and their associated adaptors recruit TANK-binding kinase 1 (TBK1) to activate interferon regulatory factor 3 (IRF3), resulting in production of type I interferons (IFNs). ICP0 and ICP34.5 are amongst the proteins encoded by herpes simplex virus 1 (HSV-1) that modulate type I IFN signaling. We constructed a recombinant virus (ΔXX) which lacks amino acids 87 - 106, a portion of the previously described TBK1-binding domain of the γ34.5 gene (D. Verpooten, Y...
September 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28866373/ros-activates-autophagy-in-follicular-granulosa-cells-via-mtor-pathway-to-regulate-broodiness-in-goose
#17
Yaping Lou, Wensai Yu, Lu Han, Songbai Yang, Yali Wang, Ting Ren, Jing Yu, Ayong Zhao
Broodiness causes reduced reproductive ability in poultry, but its regulatory mechanism remains poorly understood. ROS (reactive oxygen species) and autophagy are important for follicular development, and the interaction between the two may play a role in regulating broodiness. We examined goose follicles for ROS and oxidation scavenger activities during the egg-laying and broody stages. The follicular granulosa cells were exposed to media containing H2O2, and the interactions between ROS and autophagy in follicular granulosa cells in vitro were analyzed using a Western blot method...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28861155/calcitriol-promotes-survival-of-experimental-random-pattern-flap-via-activation-of-autophagy
#18
Long Chen, Kailiang Zhou, Huanwen Chen, Shihen Li, Dingsheng Lin, Dongsheng Zhou
Calcitriol can promote random pattern flap survival and increase autophagy activity. However, effect of autophagy on flap survival after calcitriol treatment, along with the underlying mechanism, is undefined. In this study, the "McFarlane flap" model was established in 72 rats divided into control, calcitriol, and calcitriol+3-methyladenine (3MA) groups and injected with saline, calcitriol, and calcitriol plus 3MA, respectively. The percentage flap survival area and tissue water content were tested after 7 days...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28856729/the-protein-restriction-mimetic-resveratrol-is-an-autophagy-inducer-stronger-than-amino-acid-starvation-in-ovarian-cancer-cells
#19
Alessandra Ferraresi, Rossella Titone, Carlo Follo, Andrea Castiglioni, Giovanna Chiorino, Danny N Dhanasekaran, Ciro Isidoro
The potential benefit of nutrient starvation in the prevention and treatment of cancer is presently under consideration. Resveratrol (RV), a dietary polyphenol acting as a protein (caloric) restriction mimetic, could substitute for amino acid starvation. The effects of starvation and of caloric restriction are mediated, among others, by autophagy, a process that contributes to cell homeostasis by promoting the lysosomal degradation of damaged and redundant self-constituents. Up-regulation of autophagy favors cell survival under nutrient shortage situation, and may drive cancer cells into a non-replicative, dormant state...
August 30, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28855439/intrauterine-growth-retardation-promotes-fetal-intestinal-autophagy-in-rats-via-the-mechanistic-target-of-rapamycin-pathway
#20
Chao Wang, Ruiming Zhang, Le Zhou, Jintian He, Qiang Huang, Farman A Siyal, Lili Zhang, Xiang Zhong, Tian Wang
Intrauterine growth retardation (IUGR) impairs fetal intestinal development, and is associated with high perinatal morbidity and mortality. However, the mechanism underlying this intestinal injury is largely unknown. We aimed to investigate this mechanism through analysis of intestinal autophagy and related signaling pathways in a rat model of IUGR. Normal weight (NW) and IUGR fetuses were obtained from primiparous rats via ad libitum food intake and 50% food restriction, respectively. Maternal serum parameters, fetal body weight, organ weights, and fetal blood glucose were determined...
August 31, 2017: Journal of Reproduction and Development
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