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https://www.readbyqxmd.com/read/28731223/neferine-modulates-igf-1r-nrf2-signaling-in-doxorubicin-treated-h9c2-cardiomyoblasts
#1
Lohanathan Bharathi Priya, Rathinasamy Baskaran, Chih-Yang Huang, Viswanadha Vijaya Padma
Doxorubicin (DOX) induced cardiotoxicity is a major problem during chemotherapy of cancers. DOX-mediated suppression of type 1 IGF receptor (IGF-1R) signaling leads to cardiac dysfunction. Neferine, a bisbezylisoquinoline alkaloid from the seed embryos of Nelumbo nucifera Gaertn possesses a distinct range of pharmacological properties. Herewith, the present study attempts to elucidate the protective role of neferine against DOX induced toxicity in H9c2 rat cardiomyoblast cell line model. DOX-treated H9c2 cells significantly increased mitochondrial superoxide generation, depleted cellular antioxidant status, suppressed the activation of IGF-1R signaling via PI3K/Akt/mTOR and induced autophagy by the activation of ULK1, Beclin1, Atg7 and LC3B...
July 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28730238/altered-expression-of-mtor-and-autophagy-in-term-normal-human-placentas
#2
Qiu Xia Zhang, Quan Na, Weiwei Song
Mammalian target of rapamycin (mTOR) and autophagy have been implied in trophoblast cells proliferation and invasion. This study investigated whether mTOR and autophagy were involved in placental development and fetal programming. A total of 90 term normal placentas (37-42 gestational weeks) were collected from women who underwent elective cesarean section, with large for gestational age (LGA), fetal growth restriction (FGR), and appropriate for gestational age (AGA) infants (n=30, respectively). Capillary volume density within peripheral villi significantly decreased in the FGR placentas compared with the AGA group (p<0...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28728293/-curcumin-induces-apoptosis-and-protective-autophagy-in-human-gastric-cancer-cells-with-different-degree-of-differentiation
#3
W Li, Y Zhou, J Yang, H H Zhang, S L Zhao, T Zhang, J Huo, P Zheng
Objective: To investigate the effect of curcumin on the apoptosis and autophagy of human gastric cancer cells with different degree of differentiation. Methods: Gastric cancer cell lines BGC-823 and MKN-28 were treated with curcumin at different concentrations. The effect of curcumin on cell proliferation was measured by MTT assay. Apoptosis was assessed by flow cytometry. Autophagy status was analyzed by acridine orange staining. The expression levels of apoptotic and autophagy-related proteins were detected by Western blot...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28705993/effects-of-%C3%AE-hydroxy-%C3%AE-methylbutyrate-hmb-on-skeletal-muscle-mitochondrial-content-and-dynamics-and-lipids-after-10-days-of-bed-rest-in-older-adults
#4
Robert A Standley, Giovanna Distefano, Suzette L Pereira, Min Tian, Owen J Kelly, Paul M Coen, Nicolaas E P Deutz, Robert R Wolfe, Bret H Goodpaster
Loss of muscle mass during periods of disuse likely has negative health consequences for older adults. We have previously shown that β-hydroxy-β-methylbutyrate (HMB) supplementation during 10 days of strict bed rest (BR) attenuates the loss of lean mass in older adults. To elucidate potential molecular mechanisms of HMB effects on muscle during bed rest and resistance training rehabilitation (RT), we examined mediators of skeletal muscle mitochondrial dynamics, autophagy and atrophy, and intramyocellular lipids...
July 13, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28696138/adipoq-adiponectin-induces-cytotoxic-autophagy-in-breast-cancer-cells-through-stk11-lkb1-mediated-activation-of-the-ampk-ulk1-axis
#5
Seung J Chung, Ganji Purnachandra Nagaraju, Arumugam Nagalingam, Nethaji Muniraj, Panjamurthy Kuppusamy, Alyssa Walker, Juhyung Woo, Balázs Győrffy, Ed Gabrielson, Neeraj K Saxena, Dipali Sharma
ADIPOQ/adiponectin, an adipocytokine secreted by adipocytes in the breast tumor microenvironment, negatively regulates cancer cell growth hence increased levels of ADIPOQ/adiponectin are associated with decreased breast cancer growth. However, its mechanisms of action remain largely elusive. We report that ADIPOQ/adiponectin induces a robust accumulation of autophagosomes, increases MAP1LC3B-II/LC3B-II and decreases SQSTM1/p62 in breast cancer cells. ADIPOQ/adiponectin-treated cells and xenografts exhibit increased expression of autophagy-related proteins...
July 11, 2017: Autophagy
https://www.readbyqxmd.com/read/28691313/moderate-mammalian-target-of-rapamycin-inhibition-induces-autophagy-in-htr8-svneo-cells-via-o-linked-%C3%AE-n-acetylglucosamine-signaling
#6
Qiuxia Zhang, Quan Na, Weiwei Song
AIM: Autophagy, a highly regulated process with a dual role (pro-survival or pro-death), has been implicated in adverse pregnancy outcomes. The aim of this study was to explore the mechanism whereby mammalian target of rapamycin (mTOR) signaling regulates autophagy by modulating protein O-GlcNAcylation in human trophoblasts. METHODS: HTR8/SVneo cells were incubated in serum-free medium for different time intervals or treated with varying doses of Torin1. Protein expression and cell apoptosis were detected by immunoblotting and flow cytometry, respectively...
July 10, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28684964/apigenin-attenuates-adriamycin-induced-cardiomyocyte-apoptosis-via-the-pi3k-akt-mtor-pathway
#7
Wei Yu, Huirong Sun, Wenliang Zha, Weili Cui, Ling Xu, Qing Min, Jiliang Wu
Treatment with Adriamycin (ADR) is one of the major causes of chemotherapy-induced cardiotoxicity and therefore is the principal limiting factor in the effectiveness of chemotherapy for cancer patients. Apigenin (API) has been shown to play a cardioprotective role. The present study examined the effect of API on ADR-induced cardiotoxicity in mice. Sixty male Kunming mice were randomly divided into 4 groups: a control group, ADR model group, low-dose API treatment group (125 mg·kg(-1)), and high-dose API treatment group (250 mg·kg(-1))...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28678325/tgf-%C3%AE-1-promotes-human-hepatic-carcinoma-hepg2-cells-invasion-by-upregulating-autophagy
#8
C-L Ma, S Qiao, Y-C Li, X-F Wang, R-J Sun, X Zhang, R-K Qian, S-D Song
OBJECTIVE: To study the role of TGF-β1 in autophagy and invasion ability of human hepatic carcinoma HepG2 cells. MATERIALS AND METHODS: Cultured HepG2 cells were treated with different concentrations of TGF-β1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-β1 on invasion ability of HepG2 cells was detected with transwell method. RESULTS: The results demonstrated that TGF-β1 was able to activate autophagy of HepG2 cells in a dose-dependent manner...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28676953/regulation-of-sirt1-ampk-axis-is-critically-involved-in-gallotannin-induced-senescence-and-impaired-autophagy-leading-to-cell-death-in-hepatocellular-carcinoma-cells
#9
Hee Young Kwon, Ju-Ha Kim, Bonglee Kim, Sanjay K Srivastava, Sung-Hoon Kim
Hepatocellular carcinoma (HCC) is one of the most fatal malignancies with high mortality worldwide. Here the underlying antitumor mechanism of gallotannin was elucidated in HCC cells. Gallotannin suppressed viability and colony formation, increased subG1 portion and also induced senescence via upregulation of p21, G0/G1 arrest and higher SA-β-gal activity in HepG2 and SK-Hep1 cells. However, pan-caspase inhibitor Z-VAD-FMK reversed the ability of gallotannin to activate caspase 3 at 48 h after treatment in two HCC cells...
July 4, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28676950/comparative-transcriptome-analysis-of-molecular-mechanism-underlying-gray-to-red-body-color-formation-in-red-crucian-carp-carassius-auratus-red-var
#10
Yongqin Zhang, Jinhui Liu, Liangyue Peng, Li Ren, Huiqin Zhang, Lijun Zou, Wenbin Liu, Yamei Xiao
Red crucian carp (Carassius auratus red var.) is an ornamental fish with vivid red/orange color. It has been found that the adult body color of this strain forms a gray-to-red change. In this study, skin transcriptomes of red crucian carp are first obtained for three different stages of body color development, named by gray-color (GC), color-variation (CV), and red-color (RC) stages, respectively. From the skins of GC, CV, and RC, 103,229; 108,208; and 120,184 transcripts have been identified, respectively...
July 5, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28673462/cell-free-dna-induced-alteration-of-autophagy-response-and-tlr9-signaling-their-relation-to-amelioration-of-dss-colitis
#11
Györgyi Műzes, Anna L Kiss, Zsolt Tulassay, Ferenc Sipos
BACKGROUND: The influence of cell-free DNA (fDNA) administration on the TLR9-autophagy regulatory crosstalk within inflammatory circumstances remains unclear. AIMS: To examine the immunobiologic effects of iv. fDNA injection on the TLR9-mediated autophagy response in murine DSS-colitis. METHODS: Different types of modified fDNAs were administered to DSS-colitic mice. Disease and histological activities, spleen index were measured. Changes of the TLR9-associated and autophagy-related gene expression profiles of lamina proprial cells and splenocytes were assayed by quantitative real-time PCR, and validated by immunohistochemistries...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28672940/overactivated-autophagy-contributes-to-steroid-induced-avascular-necrosis-of-the-femoral-head
#12
Xuan'An Li, Yu-Sheng Li, Liang-Jun Li, Xi Xie, Ye Yang, Zheng-Han Deng, Chao Zeng, Guang-Hua Lei
Steroid-induced avascular necrosis of the femoral head (SANFH) is a mainly bilateral complication of steroid therapy that involves extensive necrosis, and frequently occurs in young and middle-aged individuals, with a high disability rate. Autophagy is an intracellular lysosomal degradation process occurring in numerous diseases. However, the effect of dexamethasone (DXM)-induced autophagy on osteoblasts is unclear. The aim of the present study was to investigate the effects of autophagy on SANFH. In the present study, femoral head of SANFH patients was collected, and the autophagy in the samples was evaluated...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28669597/local-intra-articular-injection-of-resveratrol-delays-cartilage-degeneration-in-c57bl-6-mice-by-inducing-autophagy-via-ampk-mtor-pathway
#13
Na Qin, Liwei Wei, Wuyin Li, Wei Yang, Litao Cai, Zhuang Qian, Shufang Wu
Autophagy is an essential cellular homeostasis mechanism that was found to be compromised in aging and osteoarthritis (OA) cartilage. Previous studies showed that resveratrol can effectively regulate autophagy in other cells. The purpose of this study was to determine whether the chondroprotective effect of resveratrol was related to chondrocyte autophagy and to elucidate underlying mechanisms. OA model was induced by destabilization of the medial meniscus (DMM) in 10-week-old male mice. OA mice were treated with resveratrol with/without 3-MA for 8 weeks beginning 4 weeks after surgery...
June 15, 2017: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/28665330/effects-of-epigallocatechin-3-gallate-on-autophagic-lipolysis-in-adipocytes
#14
Sang-Nam Kim, Hyun-Jung Kwon, Seun Akindehin, Hyun Woo Jeong, Yun-Hee Lee
Previous studies demonstrated effects of green tea on weight loss; however, green tea-induced modulation of adipocyte function is not fully understood. Here, we investigated effects of the major green tea phytochemical, epigallocatechin-3-gallate (EGCG) on triglyceride contents, lipolysis, mitochondrial function, and autophagy, in adipocytes differentiated from C₃H10T1/2 cells and immortalized pre-adipocytes in vitro. EGCG reduced the triglycerol content significantly in adipocytes by 25%, comparable to the nutrient starvation state...
June 30, 2017: Nutrients
https://www.readbyqxmd.com/read/28663053/protective-effect-of-electroacupuncture-on-neurons-autophagy-in-perfusion-period-of-cerebral-ischemia
#15
Zuo Ting, Zhang Jianbin, Huang Luqi
Electroacupuncture (EA) is an important treatment method in Chinese traditional medicine. The main purpose of the investigation was to explore whether EA could improve cerebral ischemia/reperfusion (CI/R) via neurons' autophagy. 4-vessel occlusion (4-VO) was applied to establish cerebral ischemia model and reperfusion 12h, 24h and 48h respectively were conduct 3h later. TTC staining was applied to assess cerebral infarction area and the concentrations of IL-6, TNF-α, IL-1β, SOD and MDA were detected by commercial kits...
June 26, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28656199/wnt-%C3%AE-catenin-signaling-pathway-activation-reverses-gemcitabine-resistance-by-attenuating-beclin1-mediated-autophagy-in-the-mg63-human-osteosarcoma-cell-line
#16
Hao Tao, Feng Chen, Haifei Liu, Yanling Hu, Yingzhen Wang, Haiyan Li
Anaberrant Wnt/β-catenin signaling pathway is frequently implicated in tumorigenesis. However, whether the Wnt/β‑catenin pathway plays a role in resistance to antitumor chemotherapy drugs remains unknown. In the present study, the process of autophagy was assessed following overexpression of the autophagy‑associated gene Beclin 1 in gemcitabine‑induced MG63 human osteosarcoma cells. Autophagy‑associated gene expression was measured following activation or inhibition of the Wnt/β‑catenin pathway in gemcitabine‑induced MG63 cells using reverse transcription‑quantitative polymerase chain reaction...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28655725/chemical-chaperone-4-phenyl-butyric-acid-4pba-reduces-hepatocellular-lipid-accumulation-and-lipotoxicity-through-induction-of-autophagy
#17
Ashraf U Nissar, Love Sharma, Malik A Mudasir, Lone A Nazir, Sheikh A Umar, Parduman R Sharma, Ram A Vishwakarma, Sheikh A Tasduq
Defective autophagy has been linked to lipotoxicity in several cellular models. We aimed to investigate autophagy in lipid stimulated hepatoma (Huh7) cells and tested whether 4-Phenyl butyric acid (4PBA), a chemical chaperone, has a beneficial role in hepatic fat accumulation and lipotoxicity. We report that long-term (24h) exposure of hepatocytes to palmitate block autophagic flux that leads to lipid accumulation and cell death. Western blotting analysis showed increased accumulation of SQSTM1/P62, decreased expression of Beclin1 and Atg7 in palmitate-treated cells...
June 27, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28651493/mir-30d-suppresses-cell-proliferation-of-colon-cancer-cells-by-inhibiting-cell-autophagy-and-promoting-cell-apoptosis
#18
Rui Zhang, Jian Xu, Jian Zhao, Jinghui Bai
MiR-30 family plays an important role in the tumorigenesis of human cancers. The aim of the study is to investigate the role of miR-30d in human colon cancer cell lines and explore the molecular mechanism in the proliferation of colon cancer cells. The expression of miR-30d was determined by real-time polymerase chain reaction assay in colon cancer cell lines (HCT15, HCT116, HT-29, DLD-1, and SW480) and the results demonstrated that miR-30d level was significantly decreased in human colon cancer cell lines, compared with normal colon epithelial cell line...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28651104/autophagy-is-an-important-event-for-low-dose-cytarabine-treatment-in-acute-myeloid-leukemia-cells
#19
Liyun Chen, Pei Guo, Yunxiang Zhang, Xiaoyang Li, Peimin Jia, Jianhua Tong, Junmin Li
Cytarabine (Ara-c) has been an important agent in acute myeloid leukemia (AML) treatment for more than 40 years. While, the mechanisms underlying low dose cytarabine (LD Ara-c) is poorly understood. In this study, we investigated the therapeutic effect of LD Ara-C in vitro. U937 and HEL cell lines were treated with increasing dose of Ara-C and showed growth inhibition rates in a time and dose-dependent manner. Treatment with LD Ara-C (50nM) induced a time-dependent increase in expression of microtubule-associated protein light chain 3 (LC3) and beclin1, but degradation of sequestosome1 (p62) in both U937 and HEL cells...
June 16, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28647611/autophagy-suppresses-isoprenaline-induced-m2-macrophage-polarization-via-the-ros-erk-and-mtor-signaling-pathway
#20
Meihua Shan, Junfang Qin, Fengjiao Jin, Xiao Han, Haitao Guan, Xiaoge Li, Jiahui Zhang, Hongyao Zhang, Yue Wang
The objective of this study was to examine the effect of autophagy on stress-induced M2 macrophage polarization in the tumor microenvironment of breast cancer and to determine whether the underlying mechanism was related to the reactive oxygen species (ROS)/ERK and mTOR pathway. In vitro, we found that the basal autophagy level in mouse RAW 264.7 macrophages decreased with the incubation of tumor cell culture supernatant. Similarly, the polarization of RAW 264.7 to M2 macrophages was inhibited by the autophagy inducer rapamycin and increased by the autophagy inhibitor 3-MA or by siBeclin1...
June 21, 2017: Free Radical Biology & Medicine
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