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https://www.readbyqxmd.com/read/29566125/the-roles-of-id-1-in-human-pancreatic-ductal-adenocarcinoma-and-the-therapeutic-effects-of-2-methoxyestradiol
#1
Lie Yao, Xiang Zou, Chen Jin, Jie Fan, Zhongyi Guo, Quanjun Lin, Ji Li, Deliang Fu
Pancreatic ductal adenocarcinoma (PDAC) has poor prognosis and high mortality rate. Inhibitor of differentiation-1 (ID-1) overexpression has already been reported to be associated with low survival, but the detailed roles of ID-1 in PDAC are unclear. As an ID-1 inhibitor, 2-methoxyestradiol (2-ME) has been shown effective in the anti-tumor therapies, but its effect on PDAC is unknown. In this study, ID-1 overexpression and knockdown stable SW1990 cells were used to examine proliferation, migration and invasion abilities...
March 16, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29523268/the-reduction-in-circulating-levels-of-estrogen-and-progesterone-in-women-with-preeclampsia
#2
Jiayi Wan, Zheng Hu, Ke Zeng, Yongxiang Yin, Min Zhao, Miaoxin Chen, Qi Chen
Abnormalities in the development of placental vasculature in early pregnancy and the failure of transformation of the spiral arteries are associated with the pathogenesis of preeclampsia. Sex hormones influence neovascularisation during pregnancy. However the profiling of estrogen and progesterone in preeclampsia is controversial. In this study we investigated the serum levels of estrogen and progesterone in women with preeclampsia. Blood samples were collected from 86 preeclamptic and 97 gestation-matched normotensive pregnancies...
January 2018: Pregnancy Hypertension
https://www.readbyqxmd.com/read/29518001/design-and-characterization-of-dicyanovinyl-reactive-dyes-for-the-colorimetric-detection-of-thiols-and-biogenic-amines
#3
Tinkara Mastnak, Aleksandra Lobnik, Gerhard J Mohr, Matejka Turel
The synthesis of two new azobenzene dyes, namely CR-528 and CR-555, and their spectral properties in ethanol solution are described. The recognition of sulfur-containing analytes (2-mercaptoethanol (2-ME), sodium hydrosulfide (NaHS)), and biogenic amines (spermine, spermidine, ethanolamine) bestowed significant spectral changes with color changes from pink/purple to pale yellow/orange-yellow. The nitro acceptor group in the dicyanovinyl reactive dye contributes to higher sensitivity and lower detected analyte concentrations...
March 8, 2018: Sensors
https://www.readbyqxmd.com/read/29488765/halides-held-by-bifurcated-chalcogen-hydrogen-bonds-effect-of-%C3%AE-s-n-h-cl-contacts-on-dimerization-of-cl-carbene-pd-ii-species
#4
Alexander S Mikherdov, Alexander S Novikov, Mikhail A Kinzhalov, Vadim P Boyarskiy, Galina L Starova, Alexander Yu Ivanov, Vadim Yu Kukushkin
The reaction of cis-[PdCl2 (CNCy)2 ] (1) with thiazol-2-amines (2-10) leads to the C,N-chelated diaminocarbene-like complexes [PdCl{C(N(H)4,5-R2 -thiazol-2-yl)NHCy}(CNCy)] (11-14; 82-91%) in the case of 4,5-R2 -thiazol-2-amines (R, R = H, H (2), Me, Me (3), -(CH2 )4 - (4)) and benzothiazol-2-amine (5) or gives the diaminocarbene species cis-[PdCl2 {C(N(H)Cy)N(H)4-R-thiazol-2-yl}(CNCy)] (15-19; 73-93%) for the reaction with 4-aryl-substituted thiazol-2-amines (R = Ph (6), 4-MeC6 H4 (7), 4-FC6 H4 (8), 4-ClC6 H4 (9), 3,4-F2 C6 H3 (10))...
February 28, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29452850/evaluation-of-dot-blot-test-for-serological-diagnosis-of-bovine-brucellosis
#5
Carla Resende Bastos, Luis Antonio Mathias, Márcia Mariza Gomes Jusi, Renata Ferreira Dos Santos, Glaucenyra Cecília Pinheiro da Silva, Marcos Rogério André, Rosangela Zacarias Machado, Karina Paes Bürger
The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator...
February 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29434405/inclusion-of-2-mercaptoethanol-in-lysis-buffer-could-interfere-with-isolation-of-high-molecular-weight-dna-from-freshwater-microalgae
#6
Willy Yee, Janushree Nanda Kumar, Priya Devi Muthusamy
2-mercaptoethanol (2-ME), alongside polyvinylpyrrolidone is commonly used in plant DNA extractions to deal with polyphenols, which could interfere with extraction and downstream applications. 2-ME is also commonly used to denature proteins and nucleases, especially RNAses. On the contrary, we found that the presence of 2-ME in lysis buffer interfered with DNA extraction from 12 strains of freshwater microalgae, resulting in DNA with poor integrity. We also found that the TNES-urea buffer, commonly used for preservation and DNA extraction from fish, appears as effective as the SDS and CTAB buffer for some microalgae strains...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29426916/2-methoxyestradiol-protects-against-pressure-overload-induced-left-ventricular-hypertrophy
#7
Zaid H Maayah, Jody Levasseur, Ramanaguru Siva Piragasam, Ghada Abdelhamid, Jason R B Dyck, Richard P Fahlman, Arno G Siraki, Ayman O S El-Kadi
Numerous experimental studies have supported the evidence that 2-methoxyestradiol (2 ME) is a biologically active metabolite that mediates multiple effects on the cardiovascular system, largely independent of the estrogen receptor. 2 ME is a major cytochrome P450 1B1 (CYP1B1) metabolite and has been reported to have vasoprotective and anti-inflammatory actions. However, whether 2 ME would prevent cardiac hypertrophy induced by abdominal aortic constriction (AAC) has not been investigated yet. Therefore, the overall objectives of the present study were to elucidate the potential antihypertrophic effect of 2 ME and explore the mechanism(s) involved...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29405079/differential-effects-of-some-novel-synthetic-oestrogen-analogs-on-oxidative-pc12-cell-death-caused-by-serum-deprivation
#8
Irene B Sørvik, Eirik Johansson Solum, Nils A Labba, Trond Vidar Hansen, Ragnhild E Paulsen
Oestrogens with no or reduced oestrogen receptor (ER) binding properties are reported to have neuroprotective functions. However, we have previously shown that the hormonally inactive isomer of 17β-estradiol (17β-E), 17α-estradiol (17α-E), down-regulates glutathione (GSH) synthesis, and fails to rescue serum deprivation-induced cell death in the rat pheochromocytoma cell line PC12 in micromolar concentration. The present study examined cellular protective effects of new 17β-E analogs and 2-methoxyestradiol (2-ME) analogs with no or little oestrogen activity...
February 6, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29381491/investigation-of-an-antitumor-drug-delivery-system-based-on-anti-her2-antibody-conjugated-bsa-nanoparticles
#9
Nan Zhang, Jiangnan Zhang, Pei Wang, Xinyang Liu, Pengchao Huo, Yue Xu, Wenjie Chen, Hongying Xu, Qingfeng Tian
Conjugation of a monoclonal antibody with a nanoparticle often improves its specificity and drug loading in cancer therapy. In this study, we prepared a novel targeting nanodrug-delivery system using 2-methoxy-estradiol (2-ME) based on anti-human epidermal growth factor receptor 2 (HER2) antibody-modified BSA to improve the clinical application and antitumor effect of 2-ME. 2-ME-loaded albumin nanoparticles (2-ME-BSANPs) were prepared using a desolvation method and the anti-HER2 antibodies were conjugated to 2-ME-BSANPs (HER2-2-ME-BSANPs) using the coupling agent, succinimidyl 3-(2-pyridyldithio)propionate...
January 29, 2018: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29333652/establishment-and-characterization-of-a-brain-cell-line-from-kelp-grouper-epinephelus-moara
#10
X F Liu, Y H Wu, S N Wei, N Wang, Y Z Li, N W Zhang, P F Li, Q W Qin, S L Chen
A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30° C, with the maximum growth between 24 and 30° C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45...
February 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29275365/2-methoxyestradiol-affects-mitochondrial-biogenesis-pathway-and-succinate-dehydrogenase-complex-flavoprotein-subunit-a-in-osteosarcoma-cancer-cells
#11
Magdalena Gorska-Ponikowska, Alicja Kuban-Jankowska, Stephan A Eisler, Ugo Perricone, Giosuè Lo Bosco, Giampaolo Barone, Stephan Nussberger
BACKGROUND/AIM: Dysregulation of mitochondrial pathways is implicated in several diseases, including cancer. Notably, mitochondrial respiration and mitochondrial biogenesis are favored in some invasive cancer cells, such as osteosarcoma. Hence, the aim of the current work was to investigate the effects of 2-methoxyestradiol (2-ME), a potent anticancer agent, on the mitochondrial biogenesis of osteosarcoma cells. MATERIALS AND METHODS: Highly metastatic osteosarcoma 143B cells were treated with 2-ME separately or in combination with L-lactate, or with the solvent (non-treated control cells)...
January 2018: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/29214431/establishment-and-characterization-of-a-kidney-cell-line-from-kelp-grouper-epinephelus-moara
#12
Xiao-Feng Liu, Ya-Hong Wu, Shi-Na Wei, Na Wang, Peng-Fei Li, Yang-Zhen Li, Nian-Wei Zhang, Qi-Wei Qin, Song-Lin Chen
A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 °C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 °C, with optimal growth at 24 °C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42...
February 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29193609/endogenous-hormone-2-methoxyestradiol-suppresses-venous-hypertension-induced-angiogenesis-through-up-and-down-regulating-p53-and-id-1
#13
Xiang Zou, Li Zhang, Jie Yuan, Chunjie Yang, Zehan Wu, Jianping Song, Wei Zhu, Ying Mao, Liang Chen
Brain arteriovenous malformations (AVMs) which associate with angiogenesis due to local hypertension, chronic cerebral ischaemia and tissue hypoxia usually lead to haemorrhage, however, the therapeutic medicine for the disease is still lacking. 2-methoxyestradiol (2-ME) has been shown effective in the anti-angiogenic treatment. This study was conducted to examine whether and how 2-ME could improve the vascular malformations. Intracranial venous hypertension (VH) model produced in adult male Sprague-Dawley rats and culture of human umbilical vein endothelial cells (HUVECs) at the anoxia condition were used to induce in vivo and in vitro angiogenesis, respectively...
February 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29187265/development-and-evaluation-of-an-enzyme-linked-immunosorbent-assay-using-a-nonpoisonous-extraction-system-for-the-determination-of-crustacean-protein-in-processed-foods
#14
Daisuke Koizumi, Kazuya Shirota, Hiroshi Oda, Reiko Adachi, Shinobu Sakai, Hiroshi Akiyama, Tomoko Nishimaki-Mogami, Reiko Teshima
Crustacean proteins are food allergens that cause severe allergic reactions in patients with food allergies; therefore, the identification of crustaceans such as shrimp, crab, and lobster as ingredients in processed food products is mandatory in Japan. We previously developed and validated an ELISA method coupled with an extraction process using the surfactant sodium dodecyl sulfate and the reductant 2-mercaptoethanol (2-ME) to quantify crustacean protein. However, 2-ME was designated as poisonous in Japan in 2008...
November 30, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29167447/hyperoxidation-of-ether-linked-phospholipids-accelerates-neutrophil-extracellular-trap-formation
#15
Satoshi Yotsumoto, Yuito Muroi, Tatsuya Chiba, Rio Ohmura, Maki Yoneyama, Megumi Magarisawa, Kosuke Dodo, Naoki Terayama, Mikiko Sodeoka, Ryohei Aoyagi, Makoto Arita, Satoko Arakawa, Shigeomi Shimizu, Masato Tanaka
Because neutrophil extracellular trap (NET) formation is involved in the pathology of a wide variety of diseases, NET-regulating compounds are expected to be useful for the therapies of these diseases. In this study, we identified sulfasalazine (SSZ) as a potent enhancer of NET formation both in vitro and in vivo. Although SSZ did not increase the amount of ROS generated, it accelerated the generation of ether-linked oxidized phospholipids, such as PE (18;1e/15-HETE) and PC (16;0e/13-HODE). Trolox, but not 2-ME, effectively suppressed lipid oxidation and NET formation that were induced by SSZ...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29110580/clinical-significance-of-low-2-methoxyestradiol-levels-in-serum-and-tissue-of-recurrent-juvenile-onset-laryngeal-papillomatosis
#16
Danling Liu, Jiadong Wang, Yanan Xu
Objective We aim to explore the correlation between serum and tissue 2-methoxyestradiol (2-ME-2) levels and recurrence of juvenile-onset respiratory papillomatosis (JORRP). Study Design Retrospective cohort studies. Settings Laboratory of Otolaryngology, Department of Head and Neck Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University. Subjects and Methods Sixty-four patients diagnosed with JORRP in our department from January 2007 to December 2012 were enrolled. Patients were divided into recurrence and nonrecurrence groups, with 32 patients in each group...
November 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/29078954/elucidation-of-oxidation-and-degradation-products-of-oxygen-containing-fuel-components-by-combined-use-of-a-stable-isotopic-tracer-and-mass-spectrometry
#17
Marcella Frauscher, Charlotte Besser, Günter Allmaier, Nicole Dörr
In order to reveal the degradation products of oxygen-containing fuel components, in particular fatty acid methyl esters, a novel approach was developed to characterize the oxidation behaviour. Combination of artificial alteration under pressurized oxygen atmosphere, a stable isotopic tracer, and gas chromatography electron impact mass spectrometry (GC-EI-MS) was used to obtain detailed information on the formation of oxidation products of (9Z), (12Z)-octadecadienoic acid methyl ester (C18:2 ME). Thereby, biodiesel simulating model compound C18:2 ME was oxidized in a rotating pressurized vessel standardized for lubricant oxidation tests (RPVOT), i...
November 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29054710/novel-p-carborane-containing-multitarget-anticancer-agents-inspired-by-the-metabolism-of-17%C3%AE-estradiol
#18
Asako Kaise, Kiminori Ohta, Yasuyuki Endo
The female hormone 17 β-estradiol (E2) is synthesized from estrone by steroid sulfatase (STS), and metabolized into 2-methoxyestradiol (2-ME), whereby the biological activity of the latter is substantially different from that of E2. Based on the metabolic pathways of E2, a carborane-containing 2-ME mimic (1c) and its derivatives (1 and 2) were designed and synthesized as novel multitarget anticancer agents. Bissulfamate 1f exhibited potent STS-inhibitory activity and tubulin-polymerization-inhibitory activity...
December 15, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29031938/design-synthesis-and-antiproliferative-effect-of-17%C3%AE-amide-derivatives-of-2-methoxyestradiol-and-their-studies-on-pharmacokinetics
#19
Xiufang Shi, Zhihao Wang, Feng Xu, Xiang Lu, Haifeng Yao, Dandan Wu, Shuaijun Sun, Ruifang Nie, Shuo Gao, Panpan Li, Liwen Xia, Zhenzhong Zhang, Cong Wang
A series of 17β-amide-2-methoxyestradiol compounds were synthesized with an aim to enhance the antiproliferative effect of 2-methoxyestradiol. The antiproliferative activity of 2-methoxyestradiol analogs against human cancer cells was investigated. 2-methoxy-3-benzyloxy-17β-chloroacetamide-1,3,5(10)-triene (5e) and 2-methoxy-3-hydroxy-17β-butyramide-1,3,5(10)-triene (6c) had comparable or better antitumor activity than 2-methoxyestradiol. The elimination half-life of 6c (t1/2β=240.93min) is ten times longer than 2-ME and the area under the curve was seven times (AUC0-tmin=2068...
October 12, 2017: Steroids
https://www.readbyqxmd.com/read/28841308/palladium-ii-chloride-complexes-of-n-n-disubstituted-imidazole-2-thiones-syntheses-structures-and-catalytic-performances-in-suzuki-miyaura-and-sonogashira-coupling-reactions
#20
Li-Ming Zhang, Hai-Yan Li, Hong-Xi Li, David James Young, Yong Wang, Jian-Ping Lang
Reactions of PdCl2 with 2 equiv of N,N'-disubstituted-imidazole-2-thiones R1 R2 C3 N2 S (R1 = R2 = Me (1a),i Pr (1b), Cy (1c), C6 Me3 H2 (1d); R1 = Me, R2 = Ph (1e)) under the different conditions afford five mononuclear complexes trans-[(R1 R2 C3 N2 S)2 PdCl2 ] (R1 = R2 = Me (2a),i Pr (2b), Cy (2c), C6 Me3 H2 (2d); R1 = Me, R2 = Ph (2e)) and five binuclear Pd(II) complexes [(PdCl2 ){μ-(R1 R2 C3 N2 S)}]2 (R1 = R2 = Me (3a),i Pr (3b), Cy (3c), C6 Me3 H2 (3d); R1 = Me, R2 = Ph (3e)), respectively. Complexes 2a-2e are easily converted into the corresponding 3a-3e by adding equimolar PdCl2 in refluxing MeOH, while the reverse reaction is achieved at room temperature by addition of 2 equiv of 1a-1e...
September 18, 2017: Inorganic Chemistry
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