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https://www.readbyqxmd.com/read/29214431/establishment-and-characterization-of-a-kidney-cell-line-from-kelp-grouper-epinephelus-moara
#1
Xiao-Feng Liu, Ya-Hong Wu, Shi-Na Wei, Na Wang, Peng-Fei Li, Yang-Zhen Li, Nian-Wei Zhang, Qi-Wei Qin, Song-Lin Chen
A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 °C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 °C, with optimal growth at 24 °C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42...
December 6, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29193609/endogenous-hormone-2-methoxyestradiol-suppresses-venous-hypertension-induced-angiogenesis-through-up-and-down-regulating-p53-and-id-1
#2
Xiang Zou, Li Zhang, Jie Yuan, Chunjie Yang, Zehan Wu, Jianping Song, Wei Zhu, Ying Mao, Liang Chen
Brain arteriovenous malformations (AVMs) which associate with angiogenesis due to local hypertension, chronic cerebral ischaemia and tissue hypoxia usually lead to haemorrhage, however, the therapeutic medicine for the disease is still lacking. 2-methoxyestradiol (2-ME) has been shown effective in the anti-angiogenic treatment. This study was conducted to examine whether and how 2-ME could improve the vascular malformations. Intracranial venous hypertension (VH) model produced in adult male Sprague-Dawley rats and culture of human umbilical vein endothelial cells (HUVECs) at the anoxia condition were used to induce in vivo and in vitro angiogenesis, respectively...
November 29, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29187265/development-and-evaluation-of-an-enzyme-linked-immunosorbent-assay-using-a-nonpoisonous-extraction-system-for-the-determination-of-crustacean-protein-in-processed-foods
#3
Daisuke Koizumi, Kazuya Shirota, Hiroshi Oda, Reiko Adachi, Shinobu Sakai, Hiroshi Akiyama, Tomoko Nishimaki-Mogami, Reiko Teshima
Crustacean proteins are food allergens that cause severe allergic reactions in patients with food allergies; therefore, the identification of crustaceans such as shrimp, crab, and lobster as ingredients in processed food products is mandatory in Japan. We previously developed and validated an ELISA method coupled with an extraction process using the surfactant sodium dodecyl sulfate and the reductant 2-mercaptoethanol (2-ME) to quantify crustacean protein. However, 2-ME was designated as poisonous in Japan in 2008...
November 30, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29167447/hyperoxidation-of-ether-linked-phospholipids-accelerates-neutrophil-extracellular-trap-formation
#4
Satoshi Yotsumoto, Yuito Muroi, Tatsuya Chiba, Rio Ohmura, Maki Yoneyama, Megumi Magarisawa, Kosuke Dodo, Naoki Terayama, Mikiko Sodeoka, Ryohei Aoyagi, Makoto Arita, Satoko Arakawa, Shigeomi Shimizu, Masato Tanaka
Because neutrophil extracellular trap (NET) formation is involved in the pathology of a wide variety of diseases, NET-regulating compounds are expected to be useful for the therapies of these diseases. In this study, we identified sulfasalazine (SSZ) as a potent enhancer of NET formation both in vitro and in vivo. Although SSZ did not increase the amount of ROS generated, it accelerated the generation of ether-linked oxidized phospholipids, such as PE (18;1e/15-HETE) and PC (16;0e/13-HODE). Trolox, but not 2-ME, effectively suppressed lipid oxidation and NET formation that were induced by SSZ...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29110580/clinical-significance-of-low-2-methoxyestradiol-levels-in-serum-and-tissue-of-recurrent-juvenile-onset-laryngeal-papillomatosis
#5
Danling Liu, Jiadong Wang, Yanan Xu
Objective We aim to explore the correlation between serum and tissue 2-methoxyestradiol (2-ME-2) levels and recurrence of juvenile-onset respiratory papillomatosis (JORRP). Study Design Retrospective cohort studies. Settings Laboratory of Otolaryngology, Department of Head and Neck Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University. Subjects and Methods Sixty-four patients diagnosed with JORRP in our department from January 2007 to December 2012 were enrolled. Patients were divided into recurrence and nonrecurrence groups, with 32 patients in each group...
November 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/29078954/elucidation-of-oxidation-and-degradation-products-of-oxygen-containing-fuel-components-by-combined-use-of-a-stable-isotopic-tracer-and-mass-spectrometry
#6
Marcella Frauscher, Charlotte Besser, Günter Allmaier, Nicole Dörr
In order to reveal the degradation products of oxygen-containing fuel components, in particular fatty acid methyl esters, a novel approach was developed to characterize the oxidation behaviour. Combination of artificial alteration under pressurized oxygen atmosphere, a stable isotopic tracer, and gas chromatography electron impact mass spectrometry (GC-EI-MS) was used to obtain detailed information on the formation of oxidation products of (9Z), (12Z)-octadecadienoic acid methyl ester (C18:2 ME). Thereby, biodiesel simulating model compound C18:2 ME was oxidized in a rotating pressurized vessel standardized for lubricant oxidation tests (RPVOT), i...
November 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29054710/novel-p-carborane-containing-multitarget-anticancer-agents-inspired-by-the-metabolism-of-17%C3%AE-estradiol
#7
Asako Kaise, Kiminori Ohta, Yasuyuki Endo
The female hormone 17 β-estradiol (E2) is synthesized from estrone by steroid sulfatase (STS), and metabolized into 2-methoxyestradiol (2-ME), whereby the biological activity of the latter is substantially different from that of E2. Based on the metabolic pathways of E2, a carborane-containing 2-ME mimic (1c) and its derivatives (1 and 2) were designed and synthesized as novel multitarget anticancer agents. Bissulfamate 1f exhibited potent STS-inhibitory activity and tubulin-polymerization-inhibitory activity...
October 12, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29031938/design-synthesis-and-antiproliferative-effect-of-17%C3%AE-amide-derivatives-of-2-methoxyestradiol-and-their-studies-on-pharmacokinetics
#8
Xiufang Shi, Zhihao Wang, Feng Xu, Xiang Lu, Haifeng Yao, Dandan Wu, Shuaijun Sun, Ruifang Nie, Shuo Gao, Panpan Li, Liwen Xia, Zhenzhong Zhang, Cong Wang
A series of 17β-amide-2-methoxyestradiol compounds were synthesized with an aim to enhance the antiproliferative effect of 2-methoxyestradiol. The antiproliferative activity of 2-methoxyestradiol analogs against human cancer cells was investigated. 2-methoxy-3-benzyloxy-17β-chloroacetamide-1,3,5(10)-triene (5e) and 2-methoxy-3-hydroxy-17β-butyramide-1,3,5(10)-triene (6c) had comparable or better antitumor activity than 2-methoxyestradiol. The elimination half-life of 6c (t1/2β=240.93min) is ten times longer than 2-ME and the area under the curve was seven times (AUC0-tmin=2068...
October 12, 2017: Steroids
https://www.readbyqxmd.com/read/28841308/palladium-ii-chloride-complexes-of-n-n-disubstituted-imidazole-2-thiones-syntheses-structures-and-catalytic-performances-in-suzuki-miyaura-and-sonogashira-coupling-reactions
#9
Li-Ming Zhang, Hai-Yan Li, Hong-Xi Li, David James Young, Yong Wang, Jian-Ping Lang
Reactions of PdCl2 with 2 equiv of N,N'-disubstituted-imidazole-2-thiones R(1)R(2)C3N2S (R(1) = R(2) = Me (1a), (i)Pr (1b), Cy (1c), C6Me3H2 (1d); R(1) = Me, R(2) = Ph (1e)) under the different conditions afford five mononuclear complexes trans-[(R(1)R(2)C3N2S)2PdCl2] (R(1) = R(2) = Me (2a), (i)Pr (2b), Cy (2c), C6Me3H2 (2d); R(1) = Me, R(2) = Ph (2e)) and five binuclear Pd(II) complexes [(PdCl2){μ-(R(1)R(2)C3N2S)}]2 (R(1) = R(2) = Me (3a), (i)Pr (3b), Cy (3c), C6Me3H2 (3d); R(1) = Me, R(2) = Ph (3e)), respectively...
August 25, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28809416/heteroleptic-nickel-ii-dinhc-complexes-and-an-unusual-reverse-carbene-transfer-reaction-to-silver-i
#10
Han Vinh Huynh, Christine Hui Min Koh, Van Ha Nguyen
A series of rare [NiX2((Me)CCprop)] complexes bearing the cis-chelating benzimidazole-derived dicarbene ligand (Me)CCprop and varying anionic coligands (2, X = N3; 3, X = NCS; 4, X = I; 5, X = O2CCF3) have been prepared and coligand dependent structural and spectroscopic features have been evaluated. This study also revealed an unusual 'reverse' carbene transfer reaction from nickel to silver giving the disilver species [Ag2X2(μ-κ(2-Me)CCprop)] (6, X = OAc; 7, X = O2CCF3). A preliminary catalytic study of two representative Ni(II) diNHC complexes in the aqueous and phosphine-free Suzuki-Miyaura coupling reaction of aryl halides is reported as well...
August 29, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28801594/deficiency-in-catechol-o-methyltransferase-is-linked-to-a-disruption-of-glucose-homeostasis-in-mice
#11
Megumi Kanasaki, Swayam Prakash Srivastava, Fan Yang, Ling Xu, Sumiyo Kudoh, Munehiro Kitada, Norikazu Ueki, Hyoh Kim, Jinpeng Li, Satoru Takeda, Keizo Kanasaki, Daisuke Koya
2-methoxyestradiol (2-ME), an estrogen metabolite generated via catechol-o-methyltransferase (COMT), is multifunctional methoxy-catechol. Here, we report that COMT deficiency leads to glucose intolerance and 2-ME rescues COMT-deficient-associated metabolic defects. Liver COMT protein was suppressed in high fat diet (HFD)-fed or in pregnant mice. COMT suppression, by Ro41-0960 or siRNA, in HFD fed mice or in pregnant mice exacerbated glucose intolerance; 2-ME intervention ameliorated these defects. 2-ME effects on glucose tolerance were associated with AMPK phosphorylation in the liver and in islet cells...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28698966/two-skin-cell-lines-from-wild-type-and-albino-japanese-flounder-paralichthys-olivaceus-establishment-characterization-virus-susceptibility-efficient-transfection-and-application-to-albinism-study
#12
Ruoqing Wang, Nianwei Zhang, Renkai Wang, Shengpeng Wang, Na Wang
In order to provide an applicable cell platform to study fish pathology and skin pigmentation, two cell lines derived from skin tissues of wild-type and albino Japanese flounder were established and named JFSK_wt and JFSK_alb, respectively. These two cell lines were cultured for 45 passages within approximately 300 days. JFSK_wt and JFSK_alb cells were maintained in Dulbecco's Modified Eagle's Medium and Ham's F-12 Nutrient Mixture (DMEM/F12) supplemented with antibiotics, fetal bovine serum (FBS), 2-mercaptoethanol (2-Me), N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), and basic fibroblast growth factor (bFGF)...
December 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28693141/effect-of-2-methoxyestradiol-on-sk-lms-1-uterine-leiomyosarcoma-cells
#13
Ji-Sun Lee, Changhwan Ahn, Hee Young Kang, Eui-Bae Jeung
An endogenous metabolite of 17β-estradiol, 2-methoxyestradiol (2-ME), has affinity for estrogen receptors. This compound was reported to be a promising antitumor drug due to its anti-proliferative effects on a wide range of tumor cell types. Numerous previous studies have been performed to evaluate the cytotoxic effects of 2-ME on tumor cell lines in following the induction of G2/M cell cycle arrest and subsequent apoptosis. Uterine leiomyosarcoma (ULMS) is a relatively rare malignant smooth muscle cell tumor that develops in the uterus muscle layer...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28671476/molecular-group-13-metallaborates-derived-from-m-o-m-cleavage-promoted-by-bh3
#14
Erandi Bernabé-Pablo, Vojtech Jancik, Diego Martínez-Otero, Joaquín Barroso-Flores, Mónica Moya-Cabrera
The reaction of metalloxanes [{(Me)LM(OH)}2(μ-O)] [M = Al (1), Ga (2); (Me)L = CH{CMe(NAr)}2(-), Ar = 2,4,6-Me3C6H2, Me = methyl] with an excess of BH3·D (D = tetrahydrofuran (THF), SMe2) affords annular metallaborate systems achieved through M-O-M cleavage. Compound 1 led exclusively to the formation of eight-membered ring systems [{(Me)LAl(μ-O){B(O(n)Bu)}(μ-O)}2] (3) and [{(Me)LAl(μ-O)(BH)(μ-O)}2] (6), while for 2 the unprecedented six-membered ring systems [{((Me)LGa)2(μ-O)}(μ-O)2{B(O(n)Bu)}] (4) and [((Me)LGa)(μ-O)2{(BO(n)Bu)2(μ-O)}] (5) were observed...
July 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28669142/polymorphism-in-the-intron-20-of-porcine-o-linked-n-acetylglucosamine-transferase
#15
Jong Gug Kim, Dan Nonneman, Doo-Wan Kim, Sangsu Shin, Gary A Rohrer
OBJECTIVE: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of O-GlcNAc and GlcNAcylation has extensive crosstalk with phosphorylation to regulate signaling and transcription. Pig OGT is located near the region of chromosome X that affects follicle stimulating hormone level and testes size. The objective of this study was to find the variations of OGT between European and Chinese pigs. METHODS: Pigs were tested initially for polymorphism in OGT among European and Chinese pigs by polymerase chain reaction and sequencing at the U...
August 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28666718/performance-of-microbiological-serological-molecular-and-modified-seminal-plasma-methods-in-the-diagnosis-of-brucella-abortus-in-semen-and-serum-of-bovine-bulls
#16
G Nardi Júnior, J Megid, L A Mathias, L Paulin, A F Vicente, A Cortez, F J P Listoni, G H B Lara, R G Motta, M G M Chacur, F M Monteiro, M G Ribeiro
Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA)...
June 27, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28617500/decamethyltitanocene-hydride-intermediates-in-the-hydrogenation-of-the-corresponding-titanocene-%C3%AE-2-ethene-or-%C3%AE-2-alkyne-complexes-and-the-effects-of-bulkier-auxiliary-ligands
#17
Jiří Pinkas, Róbert Gyepes, Ivana Císařová, Jiří Kubišta, Michal Horáček, Karel Mach
(1)H NMR studies of reactions of titanocene [Cp*2Ti] (Cp* = η(5)-C5Me5) and its derivatives [Cp*(η(5):η(1)-C5Me4CH2)TiMe] and [Cp*2Ti(η(2)-CH2[double bond, length as m-dash]CH2)] with excess dihydrogen at room temperature and pressures lower than 1 bar revealed the formation of dihydride [Cp*2TiH2] (1) and the concurrent liberation of either methane or ethane, depending on the organometallic reactant. The subsequent slow decay of 1 yielding [Cp*2TiH] (2) was mediated by titanocene formed in situ and controlled by hydrogen pressure...
June 27, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28536143/aurora-kinase-a-promotes-ar-degradation-via-the-e3-ligase-chip
#18
Sukumar Sarkar, David L Brautigan, James M Larner
Reducing the levels of the androgen receptor (AR) is one of the most viable approaches to combat castration-resistant prostate cancer. Previously, we observed that proteasomal-dependent degradation of AR in response to 2-methoxyestradiol (2-ME) depends primarily on the E3 ligase C-terminus of HSP70-interacting protein (STUB1/CHIP). Here, 2-ME stimulation activates CHIP by phosphorylation via Aurora kinase A (AURKA). Aurora A kinase inhibitors and RNAi knockdown of Aurora A transcript selectively blocked CHIP phosphorylation and AR degradation...
August 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28511459/a-comparison-of-2-methoxyestradiol-value-in-women-with-severe-preeclampsia-versus-normotensive-pregnancy
#19
John Wantania, Ahsanuddin Attamimi, Rukmono Siswishanto
INTRODUCTION: Preeclampsia is a pregnancy complication characterized by high blood pressure and proteinuria. Endothelial dysfunction is a major theory suggested as its aetiology. It is caused by anti-angiogenic condition characterized by low Vascular Endothelial Growth Factor (VEGF). An estradiol metabolite, called 2-Methoxy Estradiol (2-ME), is produced with the help of Catechol-O-Methyltransferase (COMT). This substance has an important role in VEGF expression. A 2-ME is suppressed in women with preeclampsia...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28468327/quasi-living-polymerization-of-propene-with-an-isotactic-specific-zirconocene-catalyst
#20
Kei Nishii, Miyuki Murase, Takeshi Shiono
Propene polymerization with isotactic (iso)-specific C₂-symmetric rac-Me₂Si(2-Me-Benz(e)-Ind)₂ZrCl₂ (1) and rac-Me₂Si(2-Me-4-Ph-1-Ind)₂ZrCl₂ (2) were conducted under various conditions for achieving iso-specific living polymerization of propene. When Complex 1 was activated with trialkylaluminum-free modified methylaluminoxane (dMMAO) at -40 °C, the number-average molecular weight (Mn) linearly increased against the polymerization time to reach Mn = 704,000 within 15 min of polymerization, although the molecular weight distributions was broad (Mw/Mn < 3)...
May 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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