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https://www.readbyqxmd.com/read/29905853/2-methoxyestradiol-inhibits-hypoxia-induced-scleroderma-fibroblast-collagen-synthesis-by-phosphatidylinositol-3-kinase-akt-mtor-signalling
#1
Xing Zhou, Chaofan Liu, Jinghao Lu, Lubing Zhu, Ming Li
Objectives: To investigate the mechanism of 2-methoxyestradiol (2-ME) in inhibiting hypoxia-induced collagen synthesis of fibroblasts in SSc. Methods: The expressions of hypoxia-inducible factor 1 alpha (HIF-1α) and connective tissue growth factor (CTGF) in skin specimens derived from SSc patients and healthy volunteers were examined by immunohistochemistry. HIF-1α was knocked down by lentiviral transduction, and SSc dermal fibroblasts cultured under normoxic (21% O2) or hypoxic (1% O2) condition were treated with PI3K inhibitor LY294002, rapamycin or 2-ME (25 μM)...
June 13, 2018: Rheumatology
https://www.readbyqxmd.com/read/29888436/reactions-of-group-4-metallocene-bis-trimethylsilyl-acetylene-complexes-with-nitriles-and-isonitriles
#2
Uwe Rosenthal
This account summarizes reactions of group 4 metallocene bis(trimethylsilyl)acetylene complexes with nitriles and isonitriles. As examples several selected reactions of group 4 metallocene alkyne complexes of the type Cp'2M(η2-Me3SiC2SiMe3) (M = Ti, Zr, Hf; Cp' = Cp* = η5-pentamethylcyclopentadienyl; M = Ti, Zr; Hf; Cp' = Cp, Cp* and Cp'2 = rac-(ebthi) = rac-1,2-ethylene-1,1'-bis(η5-tetrahydroindenyl) with different nitriles and isonitriles are described following the general L-M-S principle, in which stoichiometric and catalytic reactions are influenced by ligands L, metals M and substrate substituents S...
June 10, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29852349/binding-properties-of-chiral-ruthenium-ii-complexes-%C3%AE-and-%C3%AE-ru-bpy-2-dppz-11-co-2-me-2-toward-the-triplex-rna-poly-u-%C3%A2-poly-a-poly-u
#3
Wen Ni, Xiaohua Liu, Lifeng Tan
Two chiral ruthenium(II) complexes containing ligand dppz-CO2 Me (dppz-11-CO2 Me = dipyrido[3,2-a,2',3'-c]phenazine-11-carboxylic acid methyl ester), Δ-[Ru(bpy)2 dppz-11-CO2 Me]2+ (bpy = 2,2'-bipyridine; Δ-1) and Λ-[Ru(bpy)2 dppz-11-CO2 Me]2+ (Λ-1), were synthesized and characterized. The binding of the two enantiomers with the triplex RNA poly(U)•poly(A)*poly(U) was carried out by various biophysical techniques. Analysis of the absorption and fluorescence features indicates that the binding strengths of the two enantiomers toward the triplex RNA differ only slightly from each other...
May 24, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29792695/bond-dissociation-energies-of-metallo-supramolecular-building-blocks-insight-from-fragmentation-of-selectively-self-assembled-heterometallic-metallo-supramolecular-aggregates
#4
Yvonne Lorenz, Albert Gutiérrez, Montserrat Ferrer, Marianne Engeser
A series of selectively self-assembled metallo-supramolecular square-like macrocycles with unsymmetric ditopic linkers and two different types of metal corners, i.e., {Pd(η3 -2-Me-C3 H4 )} and {M(dppp)} with dppp = 1,3-bis(diphenylphosphino)propane and M = Pd2+ or Pt2+ , have been studied in the gas phase using collision-induced dissociation. The aggregates show distinct fragmentation patterns determined by ligand length, i.e, aggregate size, and type of metal corner. Information on relative binding strength can be deduced...
May 24, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29688713/development-of-a-series-of-practical-fluorescent-chemical-tools-to-measure-ph-values-in-living-samples
#5
Shodai Takahashi, Yu Kagami, Kenjiro Hanaoka, Takuya Terai, Toru Komatsu, Tasuku Ueno, Masanobu Uchiyama, Ikuko Koyama-Honda, Noboru Mizushima, Tomohiko Taguchi, Hiroyuki Arai, Tetsuo Nagano, Yasuteru Urano
In biological systems, the pH in intracellular organelles or tissues is strictly regulated, and differences of pH are deeply related to key biological events such as protein degradation, intracellular trafficking, renal failure, and cancer. Ratiometric fluorescence imaging is useful for determination of precise pH values, but existing fluorescence probes have substantial limitations, such as inappropriate p Ka for imaging in the physiological pH range, inadequate photobleaching resistance, and insufficiently long excitation and emission wavelengths...
May 9, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29676689/intravenous-acetaminophen-for-postoperative-supratentorial-craniotomy-pain-a-prospective-randomized-double-blinded-placebo-controlled-trial
#6
Walavan Sivakumar, Michael Jensen, Julie Martinez, Michael Tanana, Nancy Duncan, Robert Hoesch, Jay K Riva-Cambrin, Craig Kilburg, Safdar Ansari, Paul A House
OBJECTIVE Acute pain control after cranial surgery is challenging. Prior research has shown that patients experience inadequate pain control post-craniotomy. The use of oral medications is sometimes delayed because of postoperative nausea, and the use of narcotics can impair the evaluation of brain function and thus are used judiciously. Few nonnarcotic intravenous (IV) analgesics exist. The authors present the results of the first prospective study evaluating the use of IV acetaminophen in patients after elective craniotomy...
April 20, 2018: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29671586/a-zirconium-photosensitizer-with-a-long-lived-excited-state-mechanistic-insight-into-photoinduced-single-electron-transfer
#7
Yu Zhang, Tia S Lee, Jeffrey L Petersen, Carsten Milsmann
Time-resolved emission spectroscopy for the luminescent zirconium complex Zr(Me PDP)2 (Me PDP = 2,6-bis(5-methyl-3-phenyl-1 H-pyrrol-2-yl)pyridine) revealed a long-lived excited state with a lifetime τ = 325 ± 10 μs. Computational studies using time-dependent density functional theory were conducted to identify the nature of the luminescent excited state as a mixed triplet intraligand/ligand-to-metal charge-transfer state. Stern-Volmer experiments showed a strong dependence of the quenching rate on the redox potential of the quencher indicating photoinduced single-electron transfer (SET) as the quenching pathway...
May 9, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29566125/the-roles-of-id-1-in-human-pancreatic-ductal-adenocarcinoma-and-the-therapeutic-effects-of-2-methoxyestradiol
#8
Lie Yao, Xiang Zou, Chen Jin, Jie Fan, Zhongyi Guo, Quanjun Lin, Ji Li, Deliang Fu
Pancreatic ductal adenocarcinoma (PDAC) has poor prognosis and high mortality rate. Inhibitor of differentiation-1 (ID-1) overexpression has already been reported to be associated with low survival, but the detailed roles of ID-1 in PDAC are unclear. As an ID-1 inhibitor, 2-methoxyestradiol (2-ME) has been shown effective in the antitumor therapies, but its effect on PDAC is unknown. In this study, ID-1 overexpression and knockdown stable SW1990 cells were used to examine proliferation, migration and invasion abilities...
May 3, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29523268/the-reduction-in-circulating-levels-of-estrogen-and-progesterone-in-women-with-preeclampsia
#9
Jiayi Wan, Zheng Hu, Ke Zeng, Yongxiang Yin, Min Zhao, Miaoxin Chen, Qi Chen
Abnormalities in the development of placental vasculature in early pregnancy and the failure of transformation of the spiral arteries are associated with the pathogenesis of preeclampsia. Sex hormones influence neovascularisation during pregnancy. However the profiling of estrogen and progesterone in preeclampsia is controversial. In this study we investigated the serum levels of estrogen and progesterone in women with preeclampsia. Blood samples were collected from 86 preeclamptic and 97 gestation-matched normotensive pregnancies...
January 2018: Pregnancy Hypertension
https://www.readbyqxmd.com/read/29518001/design-and-characterization-of-dicyanovinyl-reactive-dyes-for-the-colorimetric-detection-of-thiols-and-biogenic-amines
#10
Tinkara Mastnak, Aleksandra Lobnik, Gerhard J Mohr, Matejka Turel
The synthesis of two new azobenzene dyes, namely CR-528 and CR-555, and their spectral properties in ethanol solution are described. The recognition of sulfur-containing analytes (2-mercaptoethanol (2-ME), sodium hydrosulfide (NaHS)), and biogenic amines (spermine, spermidine, ethanolamine) bestowed significant spectral changes with color changes from pink/purple to pale yellow/orange-yellow. The nitro acceptor group in the dicyanovinyl reactive dye contributes to higher sensitivity and lower detected analyte concentrations...
March 8, 2018: Sensors
https://www.readbyqxmd.com/read/29488765/halides-held-by-bifurcated-chalcogen-hydrogen-bonds-effect-of-%C3%AE-s-n-h-cl-contacts-on-dimerization-of-cl-carbene-pd-ii-species
#11
Alexander S Mikherdov, Alexander S Novikov, Mikhail A Kinzhalov, Vadim P Boyarskiy, Galina L Starova, Alexander Yu Ivanov, Vadim Yu Kukushkin
The reaction of cis-[PdCl2 (CNCy)2 ] (1) with thiazol-2-amines (2-10) leads to the C,N-chelated diaminocarbene-like complexes [PdCl{ C(N(H)4,5-R2 -thiazol-2-yl)NHCy}(CNCy)] (11-14; 82-91%) in the case of 4,5-R2 -thiazol-2-amines (R, R = H, H (2), Me, Me (3), -(CH2 )4 - (4)) and benzothiazol-2-amine (5) or gives the diaminocarbene species cis-[PdCl2 {C(N(H)Cy)N(H)4-R-thiazol-2-yl}(CNCy)] (15-19; 73-93%) for the reaction with 4-aryl-substituted thiazol-2-amines (R = Ph (6), 4-MeC6 H4 (7), 4-FC6 H4 (8), 4-ClC6 H4 (9), 3,4-F2 C6 H3 (10))...
March 19, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29452850/evaluation-of-dot-blot-test-for-serological-diagnosis-of-bovine-brucellosis
#12
Carla Resende Bastos, Luis Antonio Mathias, Márcia Mariza Gomes Jusi, Renata Ferreira Dos Santos, Glaucenyra Cecília Pinheiro da Silva, Marcos Rogério André, Rosangela Zacarias Machado, Karina Paes Bürger
The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator...
February 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29434405/inclusion-of-2-mercaptoethanol-in-lysis-buffer-could-interfere-with-isolation-of-high-molecular-weight-dna-from-freshwater-microalgae
#13
Willy Yee, Janushree Nanda Kumar, Priya Devi Muthusamy
2-mercaptoethanol (2-ME), alongside polyvinylpyrrolidone is commonly used in plant DNA extractions to deal with polyphenols, which could interfere with extraction and downstream applications. 2-ME is also commonly used to denature proteins and nucleases, especially RNAses. On the contrary, we found that the presence of 2-ME in lysis buffer interfered with DNA extraction from 12 strains of freshwater microalgae, resulting in DNA with poor integrity. We also found that the TNES-urea buffer, commonly used for preservation and DNA extraction from fish, appears as effective as the SDS and CTAB buffer for some microalgae strains...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29426916/2-methoxyestradiol-protects-against-pressure-overload-induced-left-ventricular-hypertrophy
#14
Zaid H Maayah, Jody Levasseur, Ramanaguru Siva Piragasam, Ghada Abdelhamid, Jason R B Dyck, Richard P Fahlman, Arno G Siraki, Ayman O S El-Kadi
Numerous experimental studies have supported the evidence that 2-methoxyestradiol (2 ME) is a biologically active metabolite that mediates multiple effects on the cardiovascular system, largely independent of the estrogen receptor. 2 ME is a major cytochrome P450 1B1 (CYP1B1) metabolite and has been reported to have vasoprotective and anti-inflammatory actions. However, whether 2 ME would prevent cardiac hypertrophy induced by abdominal aortic constriction (AAC) has not been investigated yet. Therefore, the overall objectives of the present study were to elucidate the potential antihypertrophic effect of 2 ME and explore the mechanism(s) involved...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29405079/differential-effects-of-some-novel-synthetic-oestrogen-analogs-on-oxidative-pc12-cell-death-caused-by-serum-deprivation
#15
Irene B Sørvik, Eirik Johansson Solum, Nils A Labba, Trond Vidar Hansen, Ragnhild E Paulsen
Oestrogens with no or reduced oestrogen receptor (ER) binding properties are reported to have neuroprotective functions. However, we have previously shown that the hormonally inactive isomer of 17β-estradiol (17β-E), 17α-estradiol (17α-E), down-regulates glutathione (GSH) synthesis, and fails to rescue serum deprivation-induced cell death in the rat pheochromocytoma cell line PC12 in micromolar concentration. The present study examined cellular protective effects of new 17β-E analogs and 2-methoxyestradiol (2-ME) analogs with no or little oestrogen activity...
February 2018: Free Radical Research
https://www.readbyqxmd.com/read/29381491/investigation-of-an-antitumor-drug-delivery-system-based-on-anti-her2-antibody-conjugated-bsa-nanoparticles
#16
Nan Zhang, Jiangnan Zhang, Pei Wang, Xinyang Liu, Pengchao Huo, Yue Xu, Wenjie Chen, Hongying Xu, Qingfeng Tian
Conjugation of a monoclonal antibody with a nanoparticle often improves its specificity and drug loading in cancer therapy. In this study, we prepared a novel targeting nanodrug-delivery system using 2-methoxy-estradiol (2-ME) based on anti-human epidermal growth factor receptor 2 (HER2) antibody-modified BSA to improve the clinical application and antitumor effect of 2-ME. 2-ME-loaded albumin nanoparticles (2-ME-BSANPs) were prepared using a desolvation method and the anti-HER2 antibodies were conjugated to 2-ME-BSANPs (HER2-2-ME-BSANPs) using the coupling agent, succinimidyl 3-(2-pyridyldithio)propionate...
April 2018: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29333652/establishment-and-characterization-of-a-brain-cell-line-from-kelp-grouper-epinephelus-moara
#17
X F Liu, Y H Wu, S N Wei, N Wang, Y Z Li, N W Zhang, P F Li, Q W Qin, S L Chen
A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30° C, with the maximum growth between 24 and 30° C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45...
February 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29275365/2-methoxyestradiol-affects-mitochondrial-biogenesis-pathway-and-succinate-dehydrogenase-complex-flavoprotein-subunit-a-in-osteosarcoma-cancer-cells
#18
Magdalena Gorska-Ponikowska, Alicja Kuban-Jankowska, Stephan A Eisler, Ugo Perricone, Giosuè Lo Bosco, Giampaolo Barone, Stephan Nussberger
BACKGROUND/AIM: Dysregulation of mitochondrial pathways is implicated in several diseases, including cancer. Notably, mitochondrial respiration and mitochondrial biogenesis are favored in some invasive cancer cells, such as osteosarcoma. Hence, the aim of the current work was to investigate the effects of 2-methoxyestradiol (2-ME), a potent anticancer agent, on the mitochondrial biogenesis of osteosarcoma cells. MATERIALS AND METHODS: Highly metastatic osteosarcoma 143B cells were treated with 2-ME separately or in combination with L-lactate, or with the solvent (non-treated control cells)...
January 2018: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/29214431/establishment-and-characterization-of-a-kidney-cell-line-from-kelp-grouper-epinephelus-moara
#19
Xiao-Feng Liu, Ya-Hong Wu, Shi-Na Wei, Na Wang, Peng-Fei Li, Yang-Zhen Li, Nian-Wei Zhang, Qi-Wei Qin, Song-Lin Chen
A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 °C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 °C, with optimal growth at 24 °C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42...
February 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29193609/endogenous-hormone-2-methoxyestradiol-suppresses-venous-hypertension-induced-angiogenesis-through-up-and-down-regulating-p53-and-id-1
#20
Xiang Zou, Li Zhang, Jie Yuan, Chunjie Yang, Zehan Wu, Jianping Song, Wei Zhu, Ying Mao, Liang Chen
Brain arteriovenous malformations (AVMs) which associate with angiogenesis due to local hypertension, chronic cerebral ischaemia and tissue hypoxia usually lead to haemorrhage, however, the therapeutic medicine for the disease is still lacking. 2-methoxyestradiol (2-ME) has been shown effective in the anti-angiogenic treatment. This study was conducted to examine whether and how 2-ME could improve the vascular malformations. Intracranial venous hypertension (VH) model produced in adult male Sprague-Dawley rats and culture of human umbilical vein endothelial cells (HUVECs) at the anoxia condition were used to induce in vivo and in vitro angiogenesis, respectively...
February 2018: Journal of Cellular and Molecular Medicine
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