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https://www.readbyqxmd.com/read/28643490/characterization-of-extended-spectrum-%C3%AE-lactamase-genes-of-shigella-flexneri-isolates-with-fosfomycin-resistance-from-patients-in-china
#1
Yanyan Liu, Yue Cheng, Haifei Yang, Lifen Hu, Jun Cheng, Ying Ye, Jiabin Li
BACKGROUND: The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flexneri from patients in China. METHODS: A total of 263 S. flexneri isolates were collected from 34 hospitals in the Anhui Province of China during September 2012-September 2015 and screened for fosA3, fosA, and fosC2 by PCR amplification and sequencing...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28631253/portal-branch-ligation-does-not-counteract-the-inhibiting-effect-of-temsirolimus-on-extrahepatic-colorectal-metastatic-growth
#2
Sebastian Senger, Jens Sperling, Barbara Oberkircher, Martin K Schilling, Otto Kollmar, Michael D Menger, Christian Ziemann
The mTor-inhibitor temsirolimus (TEM) has potent anti-tumor activities on extrahepatic colorectal metastases. Treatment of patients with advanced disease may require portal branch ligation (PBL). While PBL can induce intrahepatic tumor growth, the effect of PBL on extrahepatic metastases under TEM treatment is unknown. Therefore, we analyzed the effects of TEM treatment on extrahepatic metastases during PBL-associated liver regeneration. GFP-transfected CT26.WT colorectal cancer cells were implanted into the dorsal skinfold chamber of BALB/c-mice...
June 19, 2017: Clinical & Experimental Metastasis
https://www.readbyqxmd.com/read/28589129/whole-genome-sequence-analysis-of-antimicrobial-resistance-genes-in-streptococcus-uberis-and-streptococcus-dysgalactiae-isolates-from-canadian-dairy-herds
#3
Julián Reyes Vélez, Marguerite Cameron, Juan Carlos Rodríguez-Lecompte, Fangfang Xia, Luke C Heider, Matthew Saab, J Trenton McClure, Javier Sánchez
The objectives of this study are to determine the occurrence of antimicrobial resistance (AMR) genes using whole-genome sequence (WGS) of Streptococcus uberis (S. uberis) and Streptococcus dysgalactiae (S. dysgalactiae) isolates, recovered from dairy cows in the Canadian Maritime Provinces. A secondary objective included the exploration of the association between phenotypic AMR and the genomic characteristics (genome size, guanine-cytosine content, and occurrence of unique gene sequences). Initially, 91 isolates were sequenced, and of these isolates, 89 were assembled...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28524864/mistranslation-can-enhance-fitness-through-purging-of-deleterious-mutations
#4
Sinisa Bratulic, Macarena Toll-Riera, Andreas Wagner
Phenotypic mutations are amino acid changes caused by mistranslation. How phenotypic mutations affect the adaptive evolution of new protein functions is unknown. Here we evolve the antibiotic resistance protein TEM-1 towards resistance on the antibiotic cefotaxime in an Escherichia coli strain with a high mistranslation rate. TEM-1 populations evolved in such strains endow host cells with a general growth advantage, not only on cefotaxime but also on several other antibiotics that ancestral TEM-1 had been unable to deactivate...
May 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28515902/sites-of-colonization-in-hospitalized-patients-with-infections-caused-by-extended-spectrum-beta-lactamase-organisms-a-prospective-cohort-study
#5
Zeina A Kanafani, Sukayna M Fadlallah, Sarah Assaf, Khalil Anouti, Kohar Annie B Kissoyan, Jad Sfeir, Tamara Nawar, Mohamad Yasmin, Ghassan M Matar
BACKGROUND: The objective of this study was to determine whether patients infected with extended-spectrum beta-lactamase (ESBL)-producing organisms are colonized at multiple body sites. METHODS: This was a prospective cohort study at a tertiary care center in Beirut, Lebanon. Hospitalized patients with infections caused by ESBL-producing organisms were included. Cultures were obtained from the primary site of infection as well as from other sites (skin, nasopharynx, urine, rectum)...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28492977/chaperone-substrate-interactions-monitored-via-a-robust-tem-1-%C3%AE-lactamase-fragment-complementation-assay
#6
Ling Bai, Wei He, Tianpeng Li, Cuiting Yang, Yingping Zhuang, Shu Quan
OBJECTIVE: To investigate the application of the TEM-1 β-lactamase protein fragment complementation assay (PCA) in detecting weak and unstable protein-protein interactions as typically observed during chaperone-assisted protein folding in the periplasm of Escherichia coli. RESULTS: The TEM-1 β-lactamase PCA system effectively captured the interactions of three pairs of chaperones and substrates. Moreover, the strength of the interactions can be quantitatively analyzed by comparing different levels of penicillin resistance, and the assay can be performed under 0...
May 10, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28485945/direct-observation-of-interactions-between-nanoparticles-and-nanoparticle-self-assembly-in-solution
#7
Shu Fen Tan, See Wee Chee, Guanhua Lin, Utkur Mirsaidov
Hierarchically organized nanoparticles (NPs) possess unique properties and are relevant to various technological applications. An important "bottom-up" strategy for building such hierarchical nanostructures is to guide the individual NPs into ordered nanoarchitectures using intermolecular interactions and external forces. However, our current understanding of the nanoscale interactions that govern such self-assembly processes usually relies on post-synthesis/assembly or indirect characterization. Theoretical models that can derive these interactions are presently constrained to systems with only a few particles or on short time scales...
May 9, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28462854/characterization-of-carbapenemases-esbls-and-plasmid-mediated-quinolone-determinants-in-carbapenem-insensitive-escherichia-coli-and-klebsiella-pneumoniae-in-riyadh-hospitals
#8
Mohamed H Al-Agamy, Abduelah Aljallal, Hesham H Radwan, Atef M Shibl
The main objective of this work was to characterize carbapenemases, extended-spectrum β-lactamases (ESBLs), and plasmid-mediated quinolone resistance (PMQR) among carbapenem-insensitive Klebsiella pneumoniae and Escherichia coli clinical isolates which were isolated from three hospitals in Riyadh. Thirty-one carbapenem-insensitive isolates (21 K. pneumoniae and 10 E. coli) were recovered from March 2014 to May 2014. Susceptibility testing and phenotypic detection tests were used to characterize the classes of β-lactamases...
April 24, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28413506/active-efflux-pump-adeb-is-involved-in-multidrug-resistance-of-acinetobacter-baumannii-induced-by-antibacterial-agents
#9
Tingting Zhang, Min Wang, Yixin Xie, Xianping Li, Zhihui Dong, Yanhua Liu, Ling Wang, Min Yang, Huan Song, Hong Cao, Wei Cao
The aim of the present study was to investigate the resistance of Acinetobacter baumannii, which was induced by cefepime (FEP), cefoperazone-sulbactam (SCF), tazobactam (TZP), levofloxacin (LEV), amikacin (AK), imipenem (IPM), and ciprofloxacin (CIP), in vitro. Multi-step drug resistance selection of 16 A. baumannii strains was performed using seven antibacterial agents (FEP, TZP, CIP, AK, IPM, SCF, and LEV). The minimum inhibitory concentration (MIC) was determined using the agar dilution method. Random amplified polymorphic DNA polymerase chain reaction was performed to analyze the genotypes and the carrying rates of aac(3)-I, aac(6')-I, ant(3)-I, aph(3)-Via, OXA-23, OXA-24, AmpC, TEM-1, metallo-β-lactamase gene (IMP), armA, rmtA, rmtB, parC, gyrA and adeB...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28412384/molecular-epidemiology-of-penicillinase-producing-neisseria-gonorrhoeae-isolates-in-france
#10
M Micaëlo, A Goubard, G La Ruche, E Denamur, O Tenaillon, E Cambau, H Jacquier, B Bercot
OBJECTIVES: Characterizing the molecular epidemiology of antibiotic resistance is crucial for a better understanding of the evolution and spread of resistance in Neisseria gonorrhoeae. Here, we examine the molecular epidemiology of penicillinase-producing N. gonorrhoeae (PPNG) isolates in France. METHODS: We investigated 176 PPNG isolates collected between 2010 and 2012 by the National Reference Centre in France. Genotyping was performed using the NG-MAST technique, blaTEM genes were Sanger-sequenced, and plasmids were characterized by PCR-typing...
April 13, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28378066/genetic-background-of-%C3%AE-lactamases-in-enterobacteriaceae-isolates-from-environmental-samples
#11
Daniele V de Oliveira, Luciana S Nunes, Afonso Luís Barth, Sueli T Van Der Sand
The prevalence of β-lactamase-producing Enterobacteriaceae has increased worldwide. Although antibiotic-resistant bacteria are usually associated with hospitals, there are a growing number of reports of resistant bacteria in other environments. Concern about resistant microorganisms outside the hospital setting highlights the need to investigate mechanisms of antibiotic resistance in isolates collected from the environment. The present study evaluated the resistance mechanism to β-lactam antibiotics in 40 isolates from hospital sewage and surface water from the Dilúvio Stream, Porto Alegre City, Southern Brazil...
April 4, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28369441/a-combination-of-mecillinam-and-amoxicillin-clavulanate-can-restore-susceptibility-of-high-level-tem-1-producing-escherichia-coli-to-mecillinam
#12
André Birgy, Marine Delecourt, Guillaume Geslain, Emilie Desselas, Marion Caseris, Mélanie Magnan, Patricia Mariani-Kurkdjian, Philippe Bidet, Stéphane Bonacorsi
Objectives: Mecillinam is recommended in France as a first-line treatment for lower urinary tract infections, due to the large increase in resistance of Escherichia coli to other oral treatments, such as co-trimoxazole or fluoroquinolones, its limited impact on faecal microbiota and its stability in the presence of numerous β-lactamases. However, we recently identified several mecillinam-resistant E. coli isolates with a high-level expression penicillinase (HEP) phenotype that merit further study...
March 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28368864/characterization-of-esbl-producing-escherichia-coli-recovered-from-companion-dogs-in-tai-an-china
#13
Song Li, Junhe Liu, Yufa Zhou, Zengmin Miao
INTRODUCTION: Animals are considered to be reservoirs of extended-spectrum beta-lactamase (ESBL)-producing bacteria, but few epidemiological data on ESBL-producing Escherichia coli urinary tract isolates in pet dogs are available in China. METHODOLOGY: This study was conducted to describe the prevalence and characterization of ESBL producers among E. coli urinary tract isolates from pet dogs in Tai'an, China. RESULTS: A total of 118 E. coli were obtained from urinary samples of 80 companion dogs suffering from acute or chronic cystitis, of which three isolates from different dogs were ESBL producers...
March 31, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28334649/binding-of-tem-1-beta-lactamase-to-beta-lactam-antibiotics-by-frontal-affinity-chromatography
#14
Xiu Chen, Yuhua Li, Yan Zhang, Jianting Yang, Liujiao Bian
TEM-1 beta-lactamases can accurately catalyze the hydrolysis of the beta-lactam rings in beta-lactam antibiotics, which make beta-lactam antibiotics lose its activity, and the prerequisite for the hydrolysis procedure in the binding interaction of TEM-1 beta-lactamases with beta-lactam antibiotics is the beta-lactam rings in beta-lactam antibiotics. Therefore, the binding of TEM-1 beta-lactamase to three beta-lactam antibiotics including penicillin G, cefalexin as well as cefoxitin was explored here by frontal affinity chromatography in combination with fluorescence spectra, adsorption and thermodynamic data in the temperature range of 278-288K under simulated physiological conditions...
April 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28293886/creation-of-antigen-dependent-%C3%AE-lactamase-fusion-protein-tethered-by-circularly-permuted-antibody-variable-domains
#15
Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, Hiroshi Ueda
Antibody-based molecular switches that are able to recognize a range of exogenous antigens can be highly useful as a versatile biosensor. However, regulating the catalytic activity of enzymes by antibodies is still hard to achieve. Here, we describe a design method of unique antibody variable region Fv introduced with two circular permutations, called Clampbody. By tethering the Clampbody to a circularly permuted TEM-1 β-lactamase (BLA), we successfully constructed a genetically encoded molecular switch Cbody-cpBLA that shows antigen-dependent catalytic activity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28281425/antibiotic-resistance-patterns-and-beta-lactamase-identification-in-escherichia-coli-isolated-from-young-children-in-rural-limpopo-province-south-africa-the-mal-ed-cohort
#16
A S DeFrancesco, N F Tanih, A Samie, R L Guerrant, P O Bessong
BACKGROUND: Antibiotic resistance is a growing problem worldwide. Mechanisms of resistance vary, and some can confer resistance to multiple classes of antibiotics. OBJECTIVE: To characterise the antibiotic resistance profiles of Escherichia coli isolates obtained from stool samples of young rural children exposed or unexposed to antibiotics. METHODOLOGY: The samples were collected from children aged 4 - 12 months who were participants in the Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development (MAL-ED) project at the South Africa research site...
February 27, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/28264855/structural-alteration-of-ompr-as-a-source-of-ertapenem-resistance-in-a-ctx-m-15-producing-escherichia-coli-o25b-h4-sequence-type-131-clinical-isolate
#17
Hervé Dupont, Pascaline Choinier, David Roche, Sandine Adiba, Megan Sookdeb, Catherine Branger, Erick Denamur, Hedi Mammeri
In this study, an ertapenem-nonsusceptible Escherichia coli isolate was investigated to determine the genetic basis for its carbapenem resistance phenotype. This clinical strain was recovered from a patient that received, 1 year previously, ertapenem to treat a cholangitis due to a carbapenem-susceptible extended-spectrum-β-lactamase (ESBL)-producing E. coli isolate. Whole-genome sequencing of these strains was performed using Illumina and single-molecule real-time sequencing technologies. It revealed that they belonged to the ST131 clonal group, had the predicted O25b:H4 serotype, and produced the CTX-M-15 and TEM-1 β-lactamases...
May 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28264645/systematic-substitutions-at-blip-position-50-result-in-changes-in-binding-specificity-for-class-a-%C3%AE-lactamases
#18
Carolyn J Adamski, Timothy Palzkill
BACKGROUND: The production of β-lactamases by bacteria is the most common mechanism of resistance to the widely prescribed β-lactam antibiotics. β-lactamase inhibitory protein (BLIP) competitively inhibits class A β-lactamases via two binding loops that occlude the active site. It has been shown that BLIP Tyr50 is a specificity determinant in that substitutions at this position result in large differential changes in the relative affinity of BLIP for class A β-lactamases. RESULTS: In this study, the effect of systematic substitutions at BLIP position 50 on binding to class A β-lactamases was examined to further explore the role of BLIP Tyr50 in modulating specificity...
March 6, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28249582/characteristics-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-fecal-samples-of-piglets-with-diarrhea-in-central-and-southern-taiwan-in-2015
#19
Wan-Chen Lee, Kuang-Sheng Yeh
BACKGROUND: The production of extended-spectrum β-lactamases (ESBLs) confer resistance to the commonly used beta-lactam antimicrobials and ESBL-producing bacteria render treatment difficulty in human and veterinary medicine. ESBL-producing bacteria have emerged in livestock in recent years, which may raise concerns regarding possible transfer of such bacteria through the food chain. The swine industry is important in Taiwan, but investigations regarding the status of ESBL in swine are limited...
March 1, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28196882/trade-offs-between-enzyme-fitness-and-solubility-illuminated-by-deep-mutational-scanning
#20
Justin R Klesmith, John-Paul Bacik, Emily E Wrenbeck, Ryszard Michalczyk, Timothy A Whitehead
Proteins are marginally stable, and an understanding of the sequence determinants for improved protein solubility is highly desired. For enzymes, it is well known that many mutations that increase protein solubility decrease catalytic activity. These competing effects frustrate efforts to design and engineer stable, active enzymes without laborious high-throughput activity screens. To address the trade-off between enzyme solubility and activity, we performed deep mutational scanning using two different screens/selections that purport to gauge protein solubility for two full-length enzymes...
February 28, 2017: Proceedings of the National Academy of Sciences of the United States of America
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