keyword
MENU ▼
Read by QxMD icon Read
search

Pol

keyword
https://www.readbyqxmd.com/read/28443684/genetic-variants-of-hiv-type-1-in-msm-in-russia
#1
Elena Kazennova, Vita Laga, Konstantin Gromov, Natalya Lebedeva, Eugenia Zhukova, Alexander Pronin, Liliya Grezina, Nataliya Dement'eva, Andrey Shemshura, Marina Bobkova
The MSM population infected with HIV is poorly studied in Russia due to stigma and discrimination. In the first years of the HIV epidemic, the only HIV genetic variant that circulated among MSM was subtype B, usually acquired abroad. Meanwhile, the massive epidemic of HIV in Russia was caused by a highly homogenic subtype A variant, AFSU (A6), and spread mainly among drug users. In this study, 155 HIV pol sequences from MSM collected during the period of 2006-2016 were analyzed. Phylogenetic analysis found that 19...
April 26, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28440748/palmitoylated-scp1-is-targeted-to-the-plasma-membrane-and-negatively-regulates-angiogenesis
#2
Peng Liao, Weichao Wang, Yu Li, Rui Wang, Jiali Jin, Weijuan Pang, Yunfei Chen, Mingyue Shen, Xinbo Wang, Dongyang Jiang, Jinjiang Pang, Mingyao Liu, Xia Lin, Xin-Hua Feng, Ping Wang, Xin Ge
SCP1 as a nuclear transcriptional regulator acts globally to silence neuronal genes and to affect the dephosphorylation of RNA Pol ll. However, we report the first finding and description of SCP1 as a plasma membrane-localized protein in various cancer cells using EGFP- or other epitope-fused SCP1. Membrane-located SCP1 dephosphorylates AKT at serine 473, leading to the abolishment of serine 473 phosphorylation that results in suppressed angiogenesis and a decreased risk of tumorigenesis. Consistently, we observed increased AKT phosphorylation and angiogenesis followed by enhanced tumorigenesis in Ctdsp1 (which encodes SCP1) gene - knockout mice...
March 31, 2017: ELife
https://www.readbyqxmd.com/read/28438400/arteriotomy-closure-devices-in-evar-tevar-and-tavr-a-systematic-review-and-meta-analysis-of-randomised-clinical-trials-and-cohort-studies
#3
REVIEW
B P Vierhout, R A Pol, M El Moumni, C J Zeebregts
OBJECTIVES: Cardiac and vascular surgery benefit from percutaneous interventions. Arteriotomy closure devices (ACDs) enable minimally invasive access to the common femoral artery (CFA). The objective of this review was to assess the differences between ACDs and surgical cut down (SCD) of the CFA regarding the number of complications, duration of surgery (DOS), and hospital length of stay (HLOS). DESIGN: A systematic literature search with predefined search terms was performed using MEDLINE, Embase, and the Cochrane Library (2000-2016)...
April 21, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28437704/conserved-rna-polymerase-ii-initiation-complex-structure
#4
REVIEW
Merle Hantsche, Patrick Cramer
Recent cryo-electron microscopic studies have arrived at atomic models of the core transcription initiation complex comprising RNA polymerase (Pol) II and the basal transcription factors TBP, TFIIA, TFIIB, TFIIE, and TFIIF. A detailed comparison of two independently derived yeast and human core initiation complex structures reveals that they are virtually identical, demonstrating the conservation of the basic transcription machinery amongst eukaryotes. The additional factors TFIID, TFIIH, and Mediator have been located on the periphery of the core initiation complex, providing the topology of the entire initiation assembly, which comprises approximately 70 polypeptides with a molecular weight of ∼4 Megadalton...
April 21, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28432790/single-molecule-visualization-of-fast-polymerase-turnover-in-the-bacterial-replisome
#5
Jacob S Lewis, Lisanne M Spenkelink, Slobodan Jergic, Elizabeth A Wood, Enrico Monachino, Nicholas P Horan, Karl E Duderstadt, Michael M Cox, Andrew Robinson, Nicholas E Dixon, Antoine M van Oijen
The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity. Although the enzymatic activities of the replicative DNA Pol III are well understood, its dynamics within the replisome are not. Here we test the accepted view that the Pol III holoenzyme remains stably associated within the replisome. We use in vitro single-molecule assays with fluorescently labeled polymerases to demonstrate that the Pol III* complex (holoenzyme lacking the β2 sliding clamp), is rapidly exchanged during processive DNA replication...
April 22, 2017: ELife
https://www.readbyqxmd.com/read/28431012/replication-and-repair-of-a-reduced-2%C3%AE-deoxyguanosine-abasic-site-interstrand-cross-link-in-human-cells
#6
Nathan E Price, Lin Li, Kent S Gates, Yinsheng Wang
Apurinic/apyrimidinic (AP) sites, or abasic sites, which are a common type of endogenous DNA damage, can forge interstrand DNA-DNA cross-links via reaction with the exocyclic amino group on a nearby 2΄-deoxyguanosine or 2΄-deoxyadenosine in the opposite strand. Here, we utilized a shuttle vector method to examine the efficiency and fidelity with which a reduced dG-AP cross-link-containing plasmid was replicated in cultured human cells. Our results showed that the cross-link constituted strong impediments to DNA replication in HEK293T cells, with the bypass efficiencies for the dG- and AP-containing strands being 40% and 20%, respectively...
April 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28430681/dangerous-liaisons-pelvic-variant-of-lemierre-syndrome-by-right-common-iliac-vein-thrombophlebitis-after-sexual-intercourse
#7
Hélène Pol, Paul Guerby, Ludwig Duazo Cassin, Elodie Chantalat, Amanda Tournemire, Martin Baujat, Lydie Porte, Olivier Parant, Fabien Vidal
No abstract text is available yet for this article.
April 20, 2017: Journal of Lower Genital Tract Disease
https://www.readbyqxmd.com/read/28428248/new-insights-into-the-tetraspanin-tspan5-using-novel-monoclonal-antibodies
#8
Julien Saint-Pol, Martine Billard, Emmanuel Dornier, Etienne Eschenbrenner, Lydia Danglot, Claude Boucheix, Stéphanie Charrin, Eric Rubinstein
Tspan5 is a member of a subgroup of tetraspanins referred to as TspanC8. These tetraspanins directly interact with the metalloprotease ADAM10, regulate its exit from the endoplasmic reticulum and subsequent trafficking, and differentially regulate its ability to cleave various substrates and activate Notch signaling. The study of Tspan5 has been limited by the lack of good antibodies. This study provides new insights into Tspan5 using new monoclonal antibodies (mAbs), including two mAbs recognizing both Tspan5 and the highly similar tetraspanin Tspan17...
April 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28427935/the-manifolded-dynamics-of-hiv-1-transmission-in-northern-alberta-a-combined-analysis-of-virus-genetic-and-public-health-data
#9
B Vrancken, D Adachi, M Benedet, A Singh, R Read, S Shafran, G D Taylor, S Houston, K Simmonds, S Plitt, C Sikora, P Lemey, C L Charlton, J W Tang
Molecular epidemiology has become a key tool for tracking infectious disease epidemics. Here, the spread of the most prevalent HIV-1 subtypes in Northern Alberta, Canada, was characterized with a Bayesian phylogenetic approach using 1146 HIV-1 pol sequences collected between 2007 and 2013 for routine clinical management purposes. Available patient metadata were qualitatively interpreted and correlated with onwards transmission using Fisher exact tests and logistic regression. Most infections were from subtypes A (n=36), B (n=815) and C (n=211)...
April 17, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28426094/sirt7-dependent-deacetylation-of-cdk9-activates-rna-polymerase-ii-transcription
#10
Maximilian F Blank, Sifan Chen, Fabian Poetz, Martina Schnölzer, Renate Voit, Ingrid Grummt
SIRT7 is an NAD+-dependent protein deacetylase that regulates cell growth and proliferation. Previous studies have shown that SIRT7 is required for RNA polymerase I (Pol I) transcription and pre-rRNA processing. Here, we took a proteomic approach to identify novel molecular targets and characterize the role of SIRT7 in non-nucleolar processes. We show that SIRT7 interacts with numerous proteins involved in transcriptional regulation and RNA metabolism, the majority of interactions requiring ongoing transcription...
March 17, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28425872/increase-in-human-immunodeficiency-virus-1-diversity-and-detection-of-various-subtypes-and-recombinants-in-north-eastern-brazil
#11
Kledoaldo Lima, Élcio Leal, Ana Maria Salustiano Cavalcanti, Daniela Medeiros Salustiano, Luzidalva Barbosa de Medeiros, Sirleide Pereira da Silva, Heloísa Ramos Lacerda
PURPOSE: Diverse human immunodeficiency virus 1 (HIV-1) subtypes and circulating recombinant forms are found in Brazil. The majority of HIV-1 molecular epidemiological studies in Brazil have been conducted in the southern and south-eastern regions of the country, although several recent studies in the north-eastern region have addressed this issue. The objective of this study was to molecularly characterize HIV-1 circulating in Pernambuco, north-eastern Brazil. METHODOLOGY: A total of 64 samples were collected from 2002 to 2003, and another 103 were collected from 2007 to 2009...
April 21, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28425774/profile-of-the-triplex-assay-for-detection-of-chlamydia-gonorrhea-and-trichomonas-using-the-bd-max%C3%A2-system
#12
Barbara Van Der Pol
Chlamydia, gonorrhea and trichomonas are the most common curable STI. Improved access to testing could reduce infection rates and prevent sequelae. Nucleic acid amplification tests are the recommend class of diagnostic assay for these infections which are often asymptomatic. Areas Covered: A description of the BD MAX™ System (MAX) and the BD MAX CT/GC/TV assay is provided along with data from a large US clinical trial. The capacity of the system for other tests and for lab developed assays is also described...
April 20, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28425582/personality-immune-response-and-reproductive-success-an-appraisal-of-the-pace-of-life-syndrome-hypothesis
#13
Karine Monceau, François-Xavier Dechaume-Moncharmont, Jérôme Moreau, Camille Lucas, Rémi Capoduro, Sébastien Motreuil, Yannick Moret
The pace-of-life syndrome (POLS) hypothesis is an extended concept of the life history theory that includes behavioural traits. The studies challenging the POLS hypothesis often focus on the relationships between a single personality trait and a physiological and/or life history traits. While pathogens represent a major selective pressure, few studies have been interested in testing relationships between behavioural syndrome, and several fitness components including immunity. The aim of this study is to address this question in the mealworm beetle, Tenebrio molitor, a model species in immunity studies...
April 20, 2017: Journal of Animal Ecology
https://www.readbyqxmd.com/read/28425429/evidence-of-horizontal-transmission-of-the-cancer-associated-steamer-retrotransposon-among-ecological-cohort-bivalve-species
#14
Ashley N Paynter, Michael J Metzger, Jocelyn A Sessa, Mark E Siddall
Bivalve specimens from legacy frozen tissue collections, and others freshly obtained, were surveyed for the presence of the Steamer long terminal repeat (LTR)-retrotransposon associated with disseminated hemic neoplasia of the soft-shelled clam Mya areneria. Of 22 species investigated using primers for the pol region, only Atlantic M. arenaria, Atlantic and North Sea razor clams Ensis directus, and Baltic clams Macoma balthica from the North Sea were found to possess copies of Steamer in their genomes. Notably, close relatives like Mya truncata and Siliqua patula did not exhibit evidence of Steamer...
April 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28424281/distinct-roles-of-vaccinia-virus-nf-kb-inhibitor-proteins-a52-b15-and-k7-in-the-immune-response
#15
Mauro Di Pilato, Ernesto Mejías-Pérez, Carlos Oscar S Sorzano, Mariano Esteban
Poxviruses use a complex strategy to escape immune control, by expressing immunomodulatory proteins that could limit their use as vaccine vectors. To test the role of poxvirus NF-κB pathway inhibitors A52, B15 and K7 in immunity, we deleted their genes in a NYVAC vaccinia virus strain that expresses HIV-1 clade C antigens. After infection of mice, ablation of A52R, B15R and K7R increased dendritic cell, natural killer cell and neutrophil migration as well as chemokine/cytokine expression. Revertant viruses for these genes confirmed their role in inhibiting the innate immune system...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28423907/effects-of-active-site-mutations-on-specificity-of-nucleobase-binding-in-human-dna-polymerase-%C3%AE
#16
Melek N Ucisik, Sharon Hammes-Schiffer
Human DNA polymerase η (Pol η) plays a vital role in protection against skin cancer caused by damage from ultraviolet light. This enzyme rescues stalled replication forks at cyclobutane thymine-thymine dimers (TTDs) by inserting nucleotides opposite these DNA lesions. Residue R61 is conserved in the Pol η enzymes across species, but the corresponding residue, as well as its neighbor S62, is different in other Y-family polymerases, Pol ι and Pol κ. Herein, R61 and S62 are mutated to their Pol ι and Pol κ counterparts...
April 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28423325/rapid-genome-wide-recruitment-of-rna-polymerase-ii-drives-transcription-splicing-and-translation-events-during-t-cell-responses
#17
Kathrin Davari, Johannes Lichti, Christian Gallus, Franziska Greulich, N Henriette Uhlenhaut, Matthias Heinig, Caroline C Friedel, Elke Glasmacher
Activation of immune cells results in rapid functional changes, but how such fast changes are accomplished remains enigmatic. By combining time courses of 4sU-seq, RNA-seq, ribosome profiling (RP), and RNA polymerase II (RNA Pol II) ChIP-seq during T cell activation, we illustrate genome-wide temporal dynamics for ∼10,000 genes. This approach reveals not only immediate-early and posttranscriptionally regulated genes but also coupled changes in transcription and translation for >90% of genes. Recruitment, rather than release of paused RNA Pol II, primarily mediates transcriptional changes...
April 18, 2017: Cell Reports
https://www.readbyqxmd.com/read/28418943/loss-of-control-over-alcohol-seeking-in-rats-depends-on-individual-vulnerability-and-duration-of-alcohol-consumption-experience
#18
Marcia Spoelder, Sylvana Pol, Boris S G Janssen, Annemarie M Baars, Louk J M J Vanderschuren, Heidi M B Lesscher
Alcohol use disorder (AUD) is characterized by excessive alcohol use and persistent alcohol seeking despite knowledge of its negative consequences. Importantly, AUD typically develops after chronic excessive alcohol use in a subgroup of individuals who drink alcohol, suggesting that AUD results from an interaction between individual vulnerability and prolonged alcohol exposure. The present study assessed the contribution of prolonged exposure to alcohol and individual levels of alcohol intake to the development of loss of control over alcohol seeking in a conditioned suppression model...
April 17, 2017: Behavioural Pharmacology
https://www.readbyqxmd.com/read/28417960/normally-lethal-amino-acid-substitutions-suppress-an-ultramutator-dna-polymerase-%C3%AE-variant
#19
Daniel G Dennis, Jill McKay-Fleisch, Kaila Eitzen, Ian Dowsett, Scott R Kennedy, Alan J Herr
In yeast, the pol3-01,L612M double mutant allele, which causes defects in DNA polymerase delta (Pol δ) proofreading (pol3-01) and nucleotide selectivity (pol3-L612M), confers an "ultramutator" phenotype that rapidly drives extinction of haploid and diploid MMR-proficient cells. Here, we investigate antimutator mutations that encode amino acid substitutions in Pol δ that suppress this lethal phenotype. We find that most of the antimutator mutations individually suppress the pol3-01 and pol3-L612M mutator phenotypes...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28415344/simple-noise-reduction-method-based-on-nonlinear-forecasting
#20
James P L Tan
Nonparametric detrending or noise reduction methods are often employed to separate trends from noisy time series when no satisfactory models exist to fit the data. However, conventional noise reduction methods depend on subjective choices of smoothing parameters. Here we present a simple multivariate noise reduction method based on available nonlinear forecasting techniques. These are in turn based on state-space reconstruction for which a strong theoretical justification exists for their use in nonparametric forecasting...
March 2017: Physical Review. E
keyword
keyword
44633
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"