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https://www.readbyqxmd.com/read/29777557/are-stable-isotope-ratios-and-oscillations-consistent-in-all-baleen-plates-along-the-filtering-apparatus-validation-of-an-increasingly-used-methodology
#1
Raquel García-Vernet, Pol Sant, Gísli Víkingsson, Asunción Borrell, Alex Aguilar
RATIONALE: Baleen plates are anatomical structures composed of inert tissue that hang from the upper jaw in mysticetes. Baleen plates may differ in size and in coloration between different segments of the filtering row or between sides of the mouth. Concern has been raised that variation in baleen plate characteristics may reflect dissimilar structural composition and growth rates liable of affecting the stable isotope ratios and their oscillation patterns. METHODS: We measured stable carbon (δ13 C values) and nitrogen (δ15 N values) isotope ratios at intervals of one centimeter along the longitudinal axis of six baleen plates collected from different positions along the mouth of a fin whale...
May 18, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29777445/a-reverse-genetics-system-for-enterovirus-d68-using-human-rna-polymerase-i
#2
Minglei Pan, Shuai Gao, Zhenwei Zhou, Keke Zhang, Sihua Liu, Zhiyun Wang, Tao Wang
Human enterovirus D68 (EV-D68) is a highly contagious virus, which causes respiratory tract infections. However, no effective vaccines are currently available for controlling EV-D68 infection. Here, we developed a reverse genetics system to recover EV-D68 minireplicons and infectious EV-D68 from transfected plasmids using the RNA polymerase I (Pol I) promoter. The EV-D68 minireplicons contained the luciferase reporter gene, which flanked by the non-coding regions of the EV-D68 RNA. The luciferase signals could be detected in cells after transfection and Pol I promoter-mediated luciferase signal was significantly stronger than that mediated by the T7 promoter...
May 17, 2018: Virus Genes
https://www.readbyqxmd.com/read/29776310/preparation-and-evaluation-of-ribonuclease-resistant-viral-hiv-rna-standards-based-on-armored-rna-technology
#3
Mohammad Gholami, Mehrdad Ravanshad, Kazem Baesi, Siamak M. Samiee, Negin Hosseini Rozbahani, Minoo Mohraz
BAckground: The human immunodeficiency virus type 1 (HIV-1) is an infectious viral agent that gradually extinguishes the immune system, resulting in acquired immune deficiency syndrome (AIDS). The aim of this study was to construct an RNA-positive control based on armored (AR) RNA technology, using HIV-1 RNA as a model. Methods: The MS2 maturase, a coat protein gene (at positions 1765 to 1787) and HIV-1 pol gene were cloned into pET-32a plasmid. The prepared plasmid was transformed into Escherichia coli strain BL2 (DE3), and the expression of the construct was induced by 1 mM of isopropyl-L-thio-D-galactopyranoside (IPTG) at 37 °C for 16 h to obtain the fabricated AR RNA...
May 19, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29774655/ctc1-stn1-coordinates-g-and-c-strand-synthesis-to-regulate-telomere-length
#4
Peili Gu, Shuting Jia, Taylor Takasugi, Eric Smith, Jayakrishnan Nandakumar, Eric Hendrickson, Sandy Chang
Coats plus (CP) is a rare autosomal recessive disorder caused by mutations in CTC1, a component of the CST (CTC1, STN1, and TEN1) complex important for telomere length maintenance. The molecular basis of how CP mutations impact upon telomere length remains unclear. The CP CTC1L1142H mutation has been previously shown to disrupt telomere maintenance. In this study, we used CRISPR/Cas9 to engineer this mutation into both alleles of HCT116 and RPE cells to demonstrate that CTC1:STN1 interaction is required to repress telomerase activity...
May 17, 2018: Aging Cell
https://www.readbyqxmd.com/read/29773209/prevalence-and-risk-factors-of-type-2-diabetes-in-insomnia-sufferers-a-study-on-1311-individuals-referred-for-sleep-examinations
#5
Matthieu Hein, Jean-Pol Lanquart, Gwénolé Loas, Philippe Hubain, Paul Linkowski
BACKGROUND: Several studies have investigated the particular relationship between insomnia and type 2 diabetes. However, few studies have investigated the prevalence and risk factors for type 2 diabetes in insomnia sufferers. The aim of this study was to examine the prevalence and risk factors of type 2 diabetes in a large sample of insomnia sufferers. METHODS: Data from 1311 insomnia sufferers recruited from the research database of the Erasme Hospital sleep laboratory were analyzed...
June 2018: Sleep Medicine
https://www.readbyqxmd.com/read/29772821/a-preliminary-analysis-of-wind-retrieval-based-on-gf-3-wave-mode-data
#6
Lei Wang, Bing Han, Xinzhe Yuan, Bin Lei, Chibiao Ding, Yulin Yao, Qi Chen
This paper presents an analysis of measurements of the normalized radar cross-(NRCS) in Wave Mode for Chinese C-band Gaofen-3(GF-3) synthetic aperture radar (SAR). Based on 2779 images from GF-3 quad-polarization SAR in Wave Mode and collocated wind vectors from ERA-Interim, this experiment verifies the feasibility of using ocean surface wind fields and VV-polarized NRCS to perform normalized calibration. The method uses well-validated empirical C-band geophysical model function (CMOD4) to estimate the calibration constant for each beam...
May 17, 2018: Sensors
https://www.readbyqxmd.com/read/29772651/properties-and-functions-of-feline-immunodeficiency-virus-gag-domains-in-virion-assembly-and-budding
#7
REVIEW
Silvia A González, José L Affranchino
Feline immunodeficiency virus (FIV) is an important cat pathogen worldwide whose biological and pathophysiological properties resemble those of human immunodeficiency virus type 1 (HIV-1). Therefore, the study of FIV not only benefits its natural host but is also useful for the development of antiviral strategies directed against HIV-1 infections in humans. FIV assembly results from the multimerization of a single but complex viral polypeptide, the Gag precursor. In this review, we will first give an overview of the current knowledge of the proteins encoded by the FIV pol , env , rev , vif, and orf-A genes, and then we will describe and discuss in detail the critical roles that each of the FIV Gag domains plays in virion morphogenesis...
May 16, 2018: Viruses
https://www.readbyqxmd.com/read/29771677/learning-based-adaptive-optimal-tracking-control-of-strict-feedback-nonlinear-systems
#8
Weinan Gao, Zhong-Ping Jiang
This paper proposes a novel data-driven control approach to address the problem of adaptive optimal tracking for a class of nonlinear systems taking the strict-feedback form. Adaptive dynamic programming (ADP) and nonlinear output regulation theories are integrated for the first time to compute an adaptive near-optimal tracker without any a priori knowledge of the system dynamics. Fundamentally different from adaptive optimal stabilization problems, the solution to a Hamilton-Jacobi-Bellman (HJB) equation, not necessarily a positive definite function, cannot be approximated through the existing iterative methods...
June 2018: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/29769289/metarrestin-a-perinucleolar-compartment-inhibitor-effectively-suppresses-metastasis
#9
Kevin J Frankowski, Chen Wang, Samarjit Patnaik, Frank J Schoenen, Noel Southall, Dandan Li, Yaroslav Teper, Wei Sun, Irawati Kandela, Deqing Hu, Christopher Dextras, Zachary Knotts, Yansong Bian, John Norton, Steve Titus, Marzena A Lewandowska, Yiping Wen, Katherine I Farley, Lesley Mathews Griner, Jamey Sultan, Zhaojing Meng, Ming Zhou, Tomas Vilimas, Astin S Powers, Serguei Kozlov, Kunio Nagashima, Humair S Quadri, Min Fang, Charles Long, Ojus Khanolkar, Warren Chen, Jinsol Kang, Helen Huang, Eric Chow, Esthermanya Goldberg, Coral Feldman, Romi Xi, Hye Rim Kim, Gary Sahagian, Susan J Baserga, Andrew Mazar, Marc Ferrer, Wei Zheng, Ali Shilatifard, Jeffrey Aubé, Udo Rudloff, Juan Jose Marugan, Sui Huang
Metastasis remains a leading cause of cancer mortality due to the lack of specific inhibitors against this complex process. To identify compounds selectively targeting the metastatic state, we used the perinuclear compartment (PNC), a complex nuclear structure associated with metastatic behaviors of cancer cells, as a phenotypic marker for a high-content screen of over 140,000 structurally diverse compounds. Metarrestin, obtained through optimization of a screening hit, disassembles PNCs in multiple cancer cell lines, inhibits invasion in vitro, suppresses metastatic development in three mouse models of human cancer, and extends survival of mice in a metastatic pancreatic cancer xenograft model with no organ toxicity or discernable adverse effects...
May 16, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29768209/influenza-virus-mounts-a-two-pronged-attack-on-host-rna-polymerase-ii-transcription
#10
David L V Bauer, Michael Tellier, Mónica Martínez-Alonso, Takayuki Nojima, Nick J Proudfoot, Shona Murphy, Ervin Fodor
Influenza virus intimately associates with host RNA polymerase II (Pol II) and mRNA processing machinery. Here, we use mammalian native elongating transcript sequencing (mNET-seq) to examine Pol II behavior during viral infection. We show that influenza virus executes a two-pronged attack on host transcription. First, viral infection causes decreased Pol II gene occupancy downstream of transcription start sites. Second, virus-induced cellular stress leads to a catastrophic failure of Pol II termination at poly(A) sites, with transcription often continuing for tens of kilobases...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29760477/functional-interplay-between-e2f7-and-ribosomal-rrna-gene-transcription-regulates-protein-synthesis
#11
Amanda S Coutts, Shonagh Munro, Nicholas B La Thangue
A prerequisite for protein synthesis is the transcription of ribosomal rRNA genes by RNA polymerase I (Pol I), which controls ribosome biogenesis. UBF (upstream binding factor) is one of the main Pol I transcription factors located in the nucleolus that activates rRNA gene transcription. E2F7 is an atypical E2F family member that acts as a transcriptional repressor of E2F target genes, and thereby contributes to cell cycle arrest. Here, we describe an unexpected role for E2F7 in regulating rRNA gene transcription...
May 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29759984/ndf-a-nucleosome-destabilizing-factor-that-facilitates-transcription-through-nucleosomes
#12
Jia Fei, Haruhiko Ishii, Marten A Hoeksema, Franz Meitinger, George A Kassavetis, Christopher K Glass, Bing Ren, James T Kadonaga
Our understanding of transcription by RNA polymerase II (Pol II) is limited by our knowledge of the factors that mediate this critically important process. Here we describe the identification of NDF, a nucleosome-destabilizing factor that facilitates Pol II transcription in chromatin. NDF has a PWWP motif, interacts with nucleosomes near the dyad, destabilizes nucleosomes in an ATP-independent manner, and facilitates transcription by Pol II through nucleosomes in a purified and defined transcription system as well as in cell nuclei...
May 14, 2018: Genes & Development
https://www.readbyqxmd.com/read/29759685/evaluation-of-the-small-aperture-intracorneal-inlay-three-year-results-from-the-cohort-of-the-u-s-food-and-drug-administration-clinical-trial
#13
John A Vukich, Daniel S Durrie, Jay S Pepose, Vance Thompson, Corina van de Pol, Ling Lin
PURPOSE: To evaluate the safety and effectiveness of the Kamra small-aperture intracorneal inlay. SETTING: Centers in North America, Europe, Asia, and Australia. DESIGN: Prospective clinical trial. METHODS: Patients with emmetropic presbyopia had intracorneal implantation of the inlay in the nondominant eye. RESULTS: The study comprised 507 patients with emmetropic presbyopia who were aged 45 to 60 years old...
May 11, 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29758758/transition-from-homogeneous-to-inhomogeneous-limit-cycles-effect-of-local-filtering-in-coupled-oscillators
#14
Tanmoy Banerjee, Debabrata Biswas, Debarati Ghosh, Biswabibek Bandyopadhyay, Jürgen Kurths
We report an interesting symmetry-breaking transition in coupled identical oscillators, namely, the continuous transition from homogeneous to inhomogeneous limit cycle oscillations. The observed transition is the oscillatory analog of the Turing-type symmetry-breaking transition from amplitude death (i.e., stable homogeneous steady state) to oscillation death (i.e., stable inhomogeneous steady state). This novel transition occurs in the parametric zone of occurrence of rhythmogenesis and oscillation death as a consequence of the presence of local filtering in the coupling path...
April 2018: Physical Review. E
https://www.readbyqxmd.com/read/29756867/strong-neutron-pairing-in-core-4n-nuclei
#15
A Revel, F M Marqués, O Sorlin, T Aumann, C Caesar, M Holl, V Panin, M Vandebrouck, F Wamers, H Alvarez-Pol, L Atar, V Avdeichikov, S Beceiro-Novo, D Bemmerer, J Benlliure, C A Bertulani, J M Boillos, K Boretzky, M J G Borge, M Caamaño, E Casarejos, W N Catford, J Cederkäll, M Chartier, L Chulkov, D Cortina-Gil, E Cravo, R Crespo, U Datta Pramanik, P Díaz Fernández, I Dillmann, Z Elekes, J Enders, O Ershova, A Estradé, F Farinon, L M Fraile, M Freer, D Galaviz, H Geissel, R Gernhäuser, P Golubev, K Göbel, J Hagdahl, T Heftrich, M Heil, M Heine, A Heinz, A Henriques, A Ignatov, H T Johansson, B Jonson, J Kahlbow, N Kalantar-Nayestanaki, R Kanungo, A Kelic-Heil, A Knyazev, T Kröll, N Kurz, M Labiche, C Langer, T Le Bleis, R Lemmon, S Lindberg, J Machado, J Marganiec, A Movsesyan, E Nacher, M Najafi, T Nilsson, C Nociforo, S Paschalis, A Perea, M Petri, S Pietri, R Plag, R Reifarth, G Ribeiro, C Rigollet, M Röder, D Rossi, D Savran, H Scheit, H Simon, I Syndikus, J T Taylor, O Tengblad, R Thies, Y Togano, P Velho, V Volkov, A Wagner, H Weick, C Wheldon, G Wilson, J S Winfield, P Woods, D Yakorev, M Zhukov, A Zilges, K Zuber
The emission of neutron pairs from the neutron-rich N=12 isotones ^{18}C and ^{20}O has been studied by high-energy nucleon knockout from ^{19}N and ^{21}O secondary beams, populating unbound states of the two isotones up to 15 MeV above their two-neutron emission thresholds. The analysis of triple fragment-n-n correlations shows that the decay ^{19}N(-1p)^{18}C^{*}→^{16}C+n+n is clearly dominated by direct pair emission. The two-neutron correlation strength, the largest ever observed, suggests the predominance of a ^{14}C core surrounded by four valence neutrons arranged in strongly correlated pairs...
April 13, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/29754955/structural-insights-into-subunits-assembly-and-the-oxyester-splicing-mechanism-of-neq-pol-split-intein
#16
Verónica Gordo, David Aparicio, Rosa Pérez-Luque, Antoni Benito, Maria Vilanova, Isabel Usón, Ignacio Fita, Marc Ribó
Split inteins are expressed as two separated subunits (N-intein and C-intein) fused to the corresponding exteins. The specific association of both intein subunits precedes protein splicing, which results in excision of the intein subunits and in ligation, by a peptide bond, of the concomitant exteins. Catalytically active intein precursors are typically too reactive for crystallization or even isolation. Neq pol is the trans-intein of the B-type DNA polymerase I split gene from hyperthermophile Nanoarchaeum equitans...
April 25, 2018: Cell Chemical Biology
https://www.readbyqxmd.com/read/29754411/a-case-study-of-nurse-practitioner-care-compared-to-general-practitioner-care-for-children-with-respiratory-tract-infections
#17
Saskia F van Vugt, Alma C van de Pol, Frits G W Cleveringa, Rebecca K Stellato, Marieke P Kappers, Niek J de Wit, Roger A M J Damoiseaux
AIM: To compare quality of care provided by nurse practitioners with care provided by general practitioners for children with respiratory tract infections in the Netherlands. BACKGROUND: Nurse practitioners increasingly manage acute conditions in general practice, with opportunities for more protocolled care. Studies on quality of nurse practitioners' care for children with respiratory tract infections are limited to the US health care system and do not take into account baseline differences in illness severity...
May 13, 2018: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/29754278/behavioral-and-noradrenergic-sensitizations-in-vulnerable-traumatized-rats-suggest-common-bases-with-substance-use-disorders
#18
Claire Le Dorze, Jean-Pol Tassin, Fréderic Chauveau, Pascale Gisquet-Verrier
The aim of the present study was to strengthen our hypothesis of a common physiological basis for post-traumatic stress disorder (PTSD) and substance use disorders. This paper investigates the possibility that rats exposed to a PTSD model exhibit noradrenergic and behavioral sensitization, as observed following repeated drugs of abuse injections. First, rats received a single prolonged stress (SPS), combining three consecutive stressors. They were then tested, 2 weeks after the trauma for PTSD-like symptoms to discriminate between vulnerable and resilient rats...
May 12, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29750364/performance-comparison-of-deep-sequencing-platforms-for-detecting-hiv-1-variants-in-the-pol-gene
#19
Florence Nicot, Nicolas Jeanne, Stéphanie Raymond, Olivier Delfour, Romain Carcenac, Caroline Lefebvre, Karine Sauné, Pierre Delobel, Jacques Izopet
The present study compares the performances of an in-house sequencing protocol on MiSeq to those of the Sanger method, and the 454 GS-FLX for detecting and quantifying drug-resistant mutations in the HIV polymerase gene (reverse transcriptase and protease). MiSeq sequencing identified all the resistance mutations detected by bulk sequencing (n=84). The MiSeq and 454 GS-FLX platforms both identified 67 drug-resistant mutations in the reverse transcriptase and protease regions, but a further 25 drug-resistant mutations were seen by only one or other of them...
May 11, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29750247/tp53-dependent-and-independent-signaling-underlies-the-pathogenesis-and-possible-prevention-of-acrofacial-dysostosis-cincinnati-type
#20
Kristin E N Watt, Cynthia L Neben, Shawn Hall, Amy E Merrill, Paul A Trainor
Ribosome biogenesis is a global process required for growth and proliferation in all cells, but disruptions in this process surprisingly lead to tissue-specific phenotypic disorders termed ribosomopathies. Pathogenic variants in the RNA Polymerase (Pol) I subunit POLR1A cause Acrofacial Dysostosis - Cincinnati type, which is characterized by craniofacial and limb anomalies. In a zebrafish model of Acrofacial Dysostosis - Cincinnati type, we demonstrate that polr1a-/- mutants exhibit deficient 47S rRNA transcription, reduced monosomes and polysomes and, consequently, defects in protein translation...
May 10, 2018: Human Molecular Genetics
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