Yaoyu Zou, Peng Lu, Juan Shi, Wen Liu, Minglan Yang, Shaoqian Zhao, Na Chen, Maopei Chen, Yingkai Sun, Aibo Gao, Qingbo Chen, Zhiguo Zhang, Qinyun Ma, Tinglu Ning, Xiayang Ying, Jiabin Jin, Xiaxing Deng, Baiyong Shen, Yifei Zhang, Bo Yuan, Sophie Kauderer, Simin Liu, Jie Hong, Ruixin Liu, Guang Ning, Weiqing Wang, Weiqiong Gu, Jiqiu Wang
BACKGROUND: IRX3 was recently reported as the effector of the FTO variants. We aimed to test IRX3's roles in the browning program and to evaluate the association between the genetic variants in IRX3 and human obesity. METHODS: IRX3 expression was examined in beige adipocytes in human and mouse models, and further validated in induced beige adipocytes. The browning capacity of primary preadipocytes was assessed with IRX3 knockdown. Luciferase reporter analysis and ChIP assay were applied to investigate IRX3's effects on UCP1 transcriptional activity...
October 2017: EBioMedicine