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https://www.readbyqxmd.com/read/29730518/prevalence-of-gbs-serotype-iii-and-identification-of-a-st-17-like-genotype-from-neonates-with-invasive-diseases-in-guangzhou-china
#1
Junyan Liu, Ruiru Xu, Huamin Zhong, Yukui Zhong, Yongqiang Xie, Lin Li, Bing Li, Dingqiang Chen, Zhenbo Xu
OBJECTIVES: The aim of this study is to understand the surveillance of Streptococcus agalactiae (GBS) serotype and genotype which help developing specific vaccine for GBS infection. METHODS: The GBS serotype was determined by strep-B-Latex rapid agglutination method and multiplex PCR assay based on the differences among serotypes. Alleles and multilocus sequence types (MLST) were determined using the GBS MLST Web site. RESULTS: Four GBS serotypes (Ia, Ib, III, and V) were identified, with serotype III which was intimately associated with purulent meningitis and sepsis, as the dominance...
May 3, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29724169/development-of-a-melting-curve-based-multiplex-real-time-pcr-assay-for-simultaneous-detection-of-streptococcus-agalactiae-and-genes-encoding-resistance-to-macrolides-and-lincosamides
#2
Eliane Saori Otaguiri, Ana Elisa Belotto Morguette, Alexandre Tadachi Morey, Eliandro Reis Tavares, Gilselena Kerbauy, Rosângela S L de Almeida Torres, Mauricio Chaves Júnior, Maria Cristina Bronharo Tognim, Viviane Monteiro Góes, Marco Aurélio Krieger, Marcia Regina Eches Perugini, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta
BACKGROUND: Streptococcus agalactiae or Group B Streptococcus (GBS) remains the leading cause of infections in newborns worldwilde. Prenatal GBS screening of pregnant women for vaginal-rectal colonization is recommended in many countries to manage appropriate intrapartum antimicrobial prophylaxis for those identified as carriers. In this study, a novel melting-curve based multiplex real-time PCR assay for the simultaneous detection of GBS and macrolide and lincosamide resistance markers was developed...
May 3, 2018: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29705915/molecular-mapping-and-inheritance-of-restoration-of-fertility-rf-in-a4-hybrid-system-in-pigeonpea-cajanus-cajan-l-millsp
#3
Rachit K Saxena, Kishan Patel, C V Sameer Kumar, Kuldeep Tyagi, K B Saxena, Rajeev K Varshney
We report molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea. We have also developed PCR-based markers amenable to low-cost genotyping to identify fertility restorer lines. Commercial hybrids in pigeonpea are based on A4 cytoplasmic male sterility (CMS) system, and their fertility restoration is one of the key prerequisites for breeding. In this context, an effort has been made to understand the genetics and identify quantitative trait loci (QTL) associated with restoration of fertility (Rf)...
April 28, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29671175/characterisation-of-bone-and-joint-infections-due-to-group-b-streptococcus-serotype-iii-sequence-type-283
#4
Sean W Ong, Timothy Barkham, Win Mar Kyaw, Hanley J Ho, Monica Chan
In 2015, an epidemic of Group B Streptococcus (GBS) serotype III sequence type 283 (ST283) disease was reported in Singapore, associated with consumption of raw freshwater fish. In this study, we further characterise the characteristics of bone and joint infections associated with ST283 GBS in adults and the differences between ST283 and non-ST283 manifestations. A retrospective study of 54 inpatients with invasive GBS disease involving bones and/or joints from 2010 to 2015 was performed. Archived isolates were identified as GBS serotype III and ST283 positive using PCR methods...
April 18, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29615986/whole-genome-sequencing-and-multiplex-qpcr-methods-to-identify-campylobacter-jejuni-encoding-cst-ii-or-cst-iii-sialyltransferase
#5
Jason M Neal-McKinney, Kun C Liu, Karen C Jinneman, Wen-Hsin Wu, Daniel H Rice
Campylobacter jejuni causes more than 2 million cases of gastroenteritis annually in the United States, and is also linked to the autoimmune sequelae Guillan-Barre syndrome (GBS). GBS often results in flaccid paralysis, as the myelin sheaths of nerve cells are degraded by the adaptive immune response. Certain strains of C. jejuni modify their lipooligosaccharide (LOS) with the addition of neuraminic acid, resulting in LOS moieties that are structurally similar to gangliosides present on nerve cells. This can trigger GBS in a susceptible host, as antibodies generated against C...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29563850/accelerating-public-sector-rice-breeding-with-high-density-kasp-markers-derived-from-whole-genome-sequencing-of-indica-rice
#6
Katherine A Steele, Mark J Quinton-Tulloch, Resham B Amgai, Rajeev Dhakal, Shambhu P Khatiwada, Darshna Vyas, Martin Heine, John R Witcombe
Few public sector rice breeders have the capacity to use NGS-derived markers in their breeding programmes despite rapidly expanding repositories of rice genome sequence data. They rely on > 18,000 mapped microsatellites (SSRs) for marker-assisted selection (MAS) using gel analysis. Lack of knowledge about target SNP and InDel variant loci has hampered the uptake by many breeders of Kompetitive allele-specific PCR (KASP), a proprietary technology of LGC genomics that can distinguish alleles at variant loci...
2018: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/29515550/comparison-of-meconium-dna-extraction-methods-for-use-in-microbiome-studies
#7
Lisa F Stinson, Jeffrey A Keelan, Matthew S Payne
The establishment of human gut microbiota commences initially in utero . Meconium-the first fecal material passed after birth-can be used to study fetal gut contents; however, processing meconium samples for microbiome studies presents significant technical challenges. Meconium hosts a low biomass microbiome, is tar-like in texture and contains high concentrations of PCR inhibitors. This study aimed to evaluate four different DNA extraction methods to elucidate the most effective method for bacterial DNA recovery and sequencing analysis from first-pass meconium...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29466521/prolonged-zika-virus-viremia-in-a-patient-with-guillain-barr%C3%A3-syndrome-in-trinidad-and-tobago
#8
Gabriel Gonzalez-Escobar, Anne Marie Valadere, Rosmond Adams, Karen Polson-Edwards, Avery Q J Hinds, Akenath Misir, C James Hospedales
An emerging mosquito-borne flavivirus, Zika virus (ZIKV) is a significant public health concern because of the syndromes associated with the infection. In addition, ZIKV is considered a major problem due to large-scale spread of the disease and the possible clinical complications for the central nervous system, especially Guillain-Barré syndrome (GBS) and microcephaly. Since the introduction of ZIKV in the Caribbean, molecular detection of the viral RNA has been utilized as a more specific and sensitive approach to demonstrating acute infection...
February 19, 2018: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/29454653/diagnostic-yield-of-real-time-polymerase-chain-reaction-in-the-diagnosis-of-intrapartum-maternal-rectovaginal-colonization-by-group-b-streptococcus-a-systematic-review-with-meta-analysis
#9
Otto Henrique May Feuerschuette, Sheila Koettker Silveira, Ana Carolina Labor Cancelier, Rosemeri Maurici da Silva, Daisson José Trevisol, Jefferson Ricardo Pereira
Group B Streptococcus is the leading etiologic factor of neonatal sepsis. Intrapartum real-time polymerase chain reaction (RT-PCR) may allow faster and more accurate detection of maternal colonization. The aim of this study was to determine the sensitivity and specificity of RT-PCR when compared to the reference standard culture in selective broth media collected from rectovaginal tract from laboring women. We selected 15 studies that included 6368 women. Papers selection, data extraction, and quality assessment were carried out by two independent researchers...
February 2, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29443461/genetic-sex-assignment-in-wild-populations-using-genotyping-by-sequencing-data-a-statistical-threshold-approach
#10
William R Stovall, Helen R Taylor, Michael Black, Stefanie Grosser, Kim Rutherford, Neil J Gemmell
Establishing the sex of individuals in wild systems can be challenging and often requires genetic testing. Genotyping-by-sequencing (GBS) and other reduced-representation DNA sequencing (RRS) protocols (e.g., RADseq, ddRAD) have enabled the analysis of genetic data on an unprecedented scale. Here, we present a novel approach for the discovery and statistical validation of sex-specific loci in GBS data sets. We used GBS to genotype 166 New Zealand fur seals (NZFS, Arctocephalus forsteri) of known sex. We retained monomorphic loci as potential sex-specific markers in the locus discovery phase...
March 2018: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29433695/zika-en-p%C3%A3-diatrie-que-retenir
#11
P Minodier
Zikavirus (ZV) is a flavivirus transmitted by Aedes mosquitoes' bites. Sexual transmission is possible. Common ZV infection is asymptomatic or associates flu-like signs. Guillain-Barre syndrome following ZV infection is rare. Foetal infection, during the first trimester of pregnancy especially, can lead to severe neurological troubles. Diagnosis relies on blood and urine RT-PCR during the acute phase, and serology later in the disease. ELISA assays false positives are reported among patients infected with another flavivirus, whereas sero-neutralization is more specific...
December 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/29432457/the-spectrum-of-neurological-disease-associated-with-zika-and-chikungunya-viruses-in-adults-in-rio-de-janeiro-brazil-a-case-series
#12
Ravi Mehta, Cristiane Nascimento Soares, Raquel Medialdea-Carrera, Mark Ellul, Marcus Tulius Texeira da Silva, Anna Rosala-Hallas, Marcia Rodrigues Jardim, Girvan Burnside, Luciana Pamplona, Maneesh Bhojak, Radhika Manohar, Gabriel Amorelli Medeiros da Silva, Marcus Vinicius Adriano, Patricia Brasil, Rita Maria Ribeiro Nogueira, Carolina Cardoso Dos Santos, Lance Turtle, Patricia Carvalho de Sequeira, David W Brown, Michael J Griffiths, Ana Maria Bispo de Filippis, Tom Solomon
BACKGROUND: During 2015-16 Brazil experienced the largest epidemic of Zika virus ever reported. This arthropod-borne virus (arbovirus) has been linked to Guillain-Barré syndrome (GBS) in adults but other neurological associations are uncertain. Chikungunya virus has caused outbreaks in Brazil since 2014 but associated neurological disease has rarely been reported here. We investigated adults with acute neurological disorders for Zika, chikungunya and dengue, another arbovirus circulating in Brazil...
February 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29425176/favipiravir-and-ribavirin-inhibit-replication-of-asian-and-african-strains-of-zika-virus-in-different-cell-models
#13
Ji-Ae Kim, Rak-Kyun Seong, Mukesh Kumar, Ok Sarah Shin
Zika virus (ZIKV) has recently emerged as a new public health threat. ZIKV infections have caused a wide spectrum of neurological diseases, such as Guillain-Barré syndrome, myelitis, meningoencephalitis, and congenital microcephaly. No effective therapies currently exist for treating patients infected with ZIKV. Herein, we evaluated the anti-viral activity of favipiravir (T-705) and ribavirin against Asian and African strains of ZIKV using different cell models, including human neuronal progenitor cells (hNPCs), human dermal fibroblasts (HDFs), human lung adenocarcinoma cells (A549) and Vero cells...
February 9, 2018: Viruses
https://www.readbyqxmd.com/read/29357841/study-on-the-persistence-of-zika-virus-zikv-in-body-fluids-of-patients-with-zikv-infection-in-brazil
#14
Guilherme Amaral Calvet, Edna Oliveira Kara, Silvana Pereira Giozza, Camila Helena Aguiar Bôtto-Menezes, Philippe Gaillard, Rafael Freitas de Oliveira Franca, Marcus Vinicius Guimarães de Lacerda, Marcia da Costa Castilho, Patrícia Brasil, Patrícia Carvalho de Sequeira, Maeve Brito de Mello, Ximena Pamela Diaz Bermudez, Kayvon Modjarrad, Robyn Meurant, Sihem Landoulsi, Adele Schwartz Benzaken, Ana Maria Bispo de Filippis, Nathalie Jeanne Nicole Broutet
BACKGROUND: Zika virus (ZIKV) has been identified in several body fluids of infected individuals. In most cases, it remained detected in blood from few days to 1 week after the onset of symptoms, and can persist longer in urine and in semen. ZIKV infection can have dramatic consequences such as microcephaly and Guillain-Barré syndrome. ZIKV sexual transmission has been documented. A better understanding of ZIKV presence and persistence across biologic compartments is needed to devise rational measures to prevent its transmission...
January 22, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29356096/investigation-of-the-role-of-campylobacter-infection-in-suspected-acute-polyradiculoneuritis-in-dogs
#15
L Martinez-Anton, M Marenda, S M Firestone, R N Bushell, G Child, A I Hamilton, S N Long, M A R Le Chevoir
BACKGROUND: Acute polyradiculoneuritis (APN) is an immune-mediated peripheral nerve disorder in dogs that shares many similarities with Guillain-Barré syndrome (GBS) in humans, in which the bacterial pathogen Campylobacter spp. now is considered to be a major triggering agent. Little information is available concerning the relationship between APN and Campylobacter spp. in dogs. HYPOTHESIS/OBJECTIVES: To estimate the association between Campylobacter spp. infection and APN...
January 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29351318/invasive-bacterial-disease-trends-and-characterization-of-group-b-streptococcal-isolates-among-young-infants-in-southern-mozambique-2001-2015
#16
Betuel Sigaúque, Miwako Kobayashi, Delfino Vubil, Ariel Nhacolo, Alberto Chaúque, Benild Moaine, Sérgio Massora, Inácio Mandomando, Tacilta Nhampossa, Quique Bassat, Fabiana Pimenta, Clara Menéndez, Maria da Gloria Carvalho, Eusebio Macete, Stephanie J Schrag
BACKGROUND: Maternal group B streptococcal (GBS) vaccines under development hold promise to prevent GBS disease in young infants. Sub-Saharan Africa has the highest estimated disease burden, although data on incidence and circulating strains are limited. We described invasive bacterial disease (IBD) trends among infants <90 days in rural Mozambique during 2001-2015, with a focus on GBS epidemiology and strain characteristics. METHODS: Community-level birth and mortality data were obtained from Manhiça's demographic surveillance system...
2018: PloS One
https://www.readbyqxmd.com/read/29317173/identification-of-a-proteomic-biomarker-associated-with-invasive-st1-serotype-vi-group-b-streptococcus-by-maldi-tof-ms
#17
Hsiao-Chuan Lin, Jang-Jih Lu, Lee-Chung Lin, Cheng-Mao Ho, Kao-Pin Hwang, Yu-Ching Liu, Chao-Jung Chen
BACKGROUND: Group B Streptococcus (GBS) is an important invasive pathogen in neonates, pregnant women and the elderly. Serotype VI GBS, which has been rarely reported globally, has emerged as a significant pathogen in Asia. However, traditional serologic latex agglutination (LA) methods may fail to type isolates that lack of or low expression of CPS. METHODS: A total of 104 GBS strains were analyzed by MALDI-TOF MS. Multiplex PCR and multilocus sequence typing (MLST) were also performed to confirm their strains...
December 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29305550/comparison-of-four-serological-methods-and-two-reverse-transcription-pcr-assays-for-diagnosis-and-surveillance-of-zika-virus-infection
#18
Angel Balmaseda, José Victor Zambrana, Damaris Collado, Nadezna García, Saira Saborío, Douglas Elizondo, Juan Carlos Mercado, Karla Gonzalez, Cristhiam Cerpas, Andrea Nuñez, Davide Corti, Jesse J Waggoner, Guillermina Kuan, Raquel Burger-Calderon, Eva Harris
Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surveillance of Zika in the Americas have been challenging due to similar clinical manifestations and extensive antibody cross-reactivity with endemic flaviviral diseases, such as dengue. We evaluated four serological and two reverse transcription-PCR (RT-PCR) methods in acute-phase (mean day, 1...
March 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29301656/cd1a-and-cd1e-gene-polymorphisms-are-not-associated-with-susceptibility-to-guillain-barr%C3%A3-syndrome-in-the-bangladeshi-population
#19
Mohammad I Rahman, Iffat Jahan, Mir M Khalid, Israt Jahan, Rijwan U Ahammad, Shamsun Nahar, Zhahirul Islam
The post-infectious autoimmune polyradiculoneuropathy Guillain-Barré syndrome (GBS) is triggered by molecular mimicry between microbial glycolipid antigens and human peripheral nerve gangliosides. Single nucleotide polymorphisms in exon 2 of CD1A (*01/*02) and CD1E (*01/*02) were assessed using PCR-RFLP; no significant differences in genotype or allele frequency were observed between 200 patients with GBS and 200 healthy controls. CD1 gene polymorphisms cannot be recognized as a susceptibility or disease-causative factor for GBS in the Bangladeshi population...
January 15, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29140242/guillain-barr%C3%A3-syndrome-acute-disseminated-encephalomyelitis-and-encephalitis-associated-with-zika-virus-infection-in-brazil-detection-of-viral-rna-and-isolation-of-virus-during-late-infection
#20
Maria Lucia Brito Ferreira, Carlos Alexandre Antunes de Brito, Álvaro José Porto Moreira, Maria Íris de Morais Machado, Adélia Henriques-Souza, Marli Tenório Cordeiro, Ernesto Torres de Azevedo Marques, Lindomar José Pena
Zika virus (ZIKV) emerged in Brazil in 2015, which was followed by an increase of Guillain-Barre Syndrome (GBS) cases. We report the epidemiological, clinical, and laboratory findings of the first six neurological cases associated with ZIKV in Brazil seen in a reference neurology hospital in Pernambuco, Brazil. In all cases, ZIKV was detected in serum and/or cerebrospinal fluid (CSF) samples. In this case series, four cases were defined as GBS, one as acute disseminated encephalomyelitis (ADEM) and the other as encephalitis...
November 2017: American Journal of Tropical Medicine and Hygiene
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