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https://www.readbyqxmd.com/read/29140242/guillain-barr%C3%A3-syndrome-acute-disseminated-encephalomyelitis-and-encephalitis-associated-with-zika-virus-infection-in-brazil-detection-of-viral-rna-and-isolation-of-virus-during-late-infection
#1
Maria Lucia Brito Ferreira, Carlos Alexandre Antunes de Brito, Álvaro José Porto Moreira, Maria Íris de Morais Machado, Adélia Henriques-Souza, Marli Tenório Cordeiro, Ernesto Torres de Azevedo Marques, Lindomar José Pena
Zika virus (ZIKV) emerged in Brazil in 2015, which was followed by an increase of Guillain-Barre Syndrome (GBS) cases. We report the epidemiological, clinical, and laboratory findings of the first six neurological cases associated with ZIKV in Brazil seen in a reference neurology hospital in Pernambuco, Brazil. In all cases, ZIKV was detected in serum and/or cerebrospinal fluid (CSF) samples. In this case series, four cases were defined as GBS, one as acute disseminated encephalomyelitis (ADEM) and the other as encephalitis...
November 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29100794/serotype-distribution-and-clinical-correlation-of-streptococcus-agalactiae-causing-invasive-disease-in-infants-and-children-in-taiwan
#2
Chiao-Wei Lo, Hao-Chuan Liu, Chien-Chung Lee, Chia-Ling Lin, Chyi-Liang Chen, Mei-Jy Jeng, Cheng-Hsun Chiu
BACKGROUND: Streptococcus agalactiae, or group B Streptococcus (GBS), remains to be one of the leading pathogens causing invasive infections in infants. METHODS: The clinical GBS isolates from sterile sites of patients younger than 18 years old were collected from October 1998 to December 2014 in two hospitals in Taiwan. Medical records were retrospectively reviewed. Every isolate was serotyped with a multiplex PCR assay. Multilocus sequence typing (MLST) was performed in representative isolates of different serotypes...
October 24, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29082442/intrapartum-group-b-streptococcus-screening-in-the-labor-ward-by-xpert%C3%A2-gbs-real-time-pcr
#3
C Plainvert, F El Alaoui, A Tazi, C Joubrel, O Anselem, M Ballon, A Frigo, C Branger, L Mandelbrot, F Goffinet, C Poyart
Group B Streptococcus (GBS) is the leading cause of neonatal infections in industrialized countries. Intrapartum antibiotic prophylaxis (IAP) given to colonized parturients is a key step for the prevention of neonatal early-onset infection. We compared the performances of Xpert® GBS polymerase chain reaction (PCR) (Cepheid, Sunnyvale, CA, USA) as a point-of-care system in labor wards to standard culture for intrapartum GBS detection. Pregnant women with a GBS-positive antenatal screening were prospectively included...
October 29, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29036322/tgbs%C3%A2-genotyping-by-sequencing-enables-reliable-genotyping-of-heterozygous-loci
#4
Alina Ott, Sanzhen Liu, James C Schnable, Cheng-Ting 'Eddy' Yeh, Kai-Sin Wang, Patrick S Schnable
Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than (double-stranded) adaptors are ligated. This strategy ensures that only double-digested fragments are amplified and sequenced. The use of oligos avoids the necessity of preparing adaptors and the problems associated with inter-adaptor annealing/ligation...
September 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29020245/guillain-barr%C3%A3-syndrome-associated-with-zika-virus-infection-in-martinique-in-2016-a-prospective-study
#5
Benoît Rozé, Fatiha Najioullah, Jean-Louis Fergé, Frédérique Dorléans, Kossivi Apetse, Jose-Luis Barnay, Elise Daudens-Vaysse, Yannick Brouste, Raymond Césaire, Laurence Fagour, Ruddy Valentino, Martine Ledrans, Hossein Mehdaoui, Sylvie Abel, Isabelle Leparc-Goffart, Aissatou Signate, André Cabié
Background: Guillain-Barré syndrome (GBS) has been reported to be associated with Zika virus (ZIKV) infection in case reports and retrospective studies, mostly on the basis of serological tests, with the problematic cross-reacting antibodies of the Flavivirus genus. Some GBS cases do not exhibit a high level of diagnostic certainty. This prospective study aimed to describe the clinical profiles and the frequency of GBS associated with ZIKV during the ZIKV outbreak in Martinique in 2016...
October 16, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28982157/genotyping-by-sequencing-reveals-the-interspecific-c-maxima-c-reticulata-admixture-along-the-genomes-of-modern-citrus-varieties-of-mandarins-tangors-tangelos-orangelos-and-grapefruits
#6
Amel Oueslati, Amel Salhi-Hannachi, François Luro, Hélène Vignes, Pierre Mournet, Patrick Ollitrault
The mandarin horticultural group is an important component of world citrus production for the fresh fruit market. This group formerly classified as C. reticulata is highly polymorphic and recent molecular studies have suggested that numerous cultivated mandarins were introgressed by C. maxima (the pummelos). C. maxima and C. reticulata are also the ancestors of sweet and sour oranges, grapefruit, and therefore of all the "small citrus" modern varieties (mandarins, tangors, tangelos) derived from sexual hybridization between these horticultural groups...
2017: PloS One
https://www.readbyqxmd.com/read/28958396/incidence-of-guillain-barr%C3%A3-syndrome-at-a-secondary-centre-during-the-2016-zika-outbreak
#7
L Del Carpio Orantes, F J Juárez Rangel, S García-Méndez
INTRODUCTION: Although Guillain-Barré syndrome is rare, a marked increase in incidence was observed during the 2016 Zika outbreak in the Americas, with no direct causal relationship being apparent in all cases. METHODS: Cases of febrile exanthematous disease and acute flaccid paralysis were reviewed during the period from 1 August to 15 December, 2016; clinical and biochemical assessment was performed for each case to identify Zika-related Guillain-Barré syndrome...
September 25, 2017: Neurología: Publicación Oficial de la Sociedad Española de Neurología
https://www.readbyqxmd.com/read/28939394/enzyme-linked-immunosorbent-assay-for-group-a-streptococcal-anti-dnase-b-in-human-sera-using-recombinant-proteins-comparison-to-the-dna-methyl-green-micromethod
#8
Sarita Das, T Dileepan, D R Johnson, E L Kaplan, P Patrick Cleary
Among the four known Streptococcal nucleases comprising of DNase A, B, C and D; DNase B is the most common, and determination of the levels of antibody to DNase B (ADB) is often used to confirm a clinical diagnosis of Streptococcus pyogenes/group A Streptococcal (GAS) infection. The commonly used assays for antibodies that neutralize DNase B or streptolysin O activity use partially purified antigens that often fail to detect antibody changes subsequent to culture documented infections. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed employing his-tagged recombinant DNase B as plate antigen for comparison to the commonly used DNA methyl green micromethod (DMGM)...
September 20, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28928763/comparative-mapping-and-candidate-gene-analysis-of-ssiia-associated-with-grain-amylopectin-content-in-barley-hordeum-vulgare-l
#9
Xiangyun Fan, Juan Zhu, Wenbin Dong, Yuandong Sun, Chao Lv, Baojian Guo, Rugen Xu
Amylopectin concentration in barley endosperm has important effects on grain quality and end-use. In this study, quantitative trait locus (QTL) analysis together with genome-wide association studies (GWAS) were performed to identify markers linked to grain amylopectin content respectively using a doubled haploid (DH) population of 178 lines and a collection of 185 diverse barley germplasms both genotyped by genotyping-by-sequencing (GBS). A stable QTL on chromosome 7H and 11 associated single nucleotide polymorphisms (SNPs) were detected...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28923133/genetic-evidence-for-a-novel-variant-of-the-pilus-island-1-backbone-protein-in-group-b-streptococcus
#10
Sarah Teatero, Alefiya Neemuchwala, Kunlin Yang, Janice Gomes, Taryn B T Athey, Irene Martin, Walter Demczuk, Allison McGeer, Nahuel Fittipaldi
PURPOSE: Pili contribute significantly to the pathogenesis of infection of group B Streptococcus (GBS) by facilitating adhesion and invasion of host cells. GBS pilin subunits (the backbone pilin protein, BP, and the ancillary pilin proteins, AP) as well as the specific enzymes required for pilus assembly are encoded by genes located in two separate genomic regions, known as pilus island 1 (PI-1) and PI-2. Our aim was to characterize the pilus profile of a collection of GBS isolates from metropolitan Toronto, Canada...
October 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28920845/modified-use-of-real-time-pcr-detection-of-group-b-streptococcus-in-pregnancy
#11
Ali A Rabaan, Justin V Saunar, Ali M Bazzi, Joan L Soriano
The CDC recommends antenatal screening of vaginal/rectal samples for Streptococcus agalactiae at 35-37 weeks' gestation, with intra-partum antibiotic prophylaxis for positive cases. We tested a modified use of the Cepheid Xpert GBS real-time PCR kit on enrichment cultures from 554 vaginal/rectal swabs compared to the current subculturing gold standard method. Swabs were inoculated on polymyxin nalidixic acid agar plates, and Todd-Hewitt enrichment broth cultures were examined daily for growth. Todd-Hewitt broth culture was also used for Xpert GBS...
October 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28916318/matrix-metalloproteinases-2-and-9-in-campylobacter-jejuni-induced-paralytic-neuropathy-resembling-guillain-barr%C3%A3-syndrome-in-chickens
#12
Kishan Kumar Nyati, Kashi Nath Prasad, Vinita Agrawal, Nuzhat Husain
Inflammation in Guillain-Barré syndrome (GBS) is manifested by changes in matrix metalloproteinase (MMP) and pro-inflammatory cytokine expression. We investigated the expression of MMP-2, -9 and TNF-α and correlated it with pathological changes in sciatic nerve tissue from Campylobacter jejuni-induced chicken model for GBS. Campylobacter jejuni and placebo were fed to chickens and assessed for disease symptoms. Sciatic nerves were examined by histopathology and immunohistochemistry. Expressions of MMPs and TNF-α, were determined by real-time PCR, and activities of MMPs by zymography...
October 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28870726/analysis-of-the-prophages-carried-by-human-infecting-isolates-provides-new-insight-into-the-evolution-of-group-b-streptococcus-species
#13
Nathalie van der Mee-Marquet, Seydina M Diene, Laurie Barbera, Luka Courtier-Martinez, Louise Lafont, Alice Ouachée, Anne-Sophie Valentin, Sandra Dos Santos, Roland Quentin, Patrice François
OBJECTIVES: Group B Streptococcus (GBS) emerged in the 1970s as a major cause of neonatal infections, and has been increasingly associated with infections in adults since the 1990s. Prophages have been suspected to have driven these epidemiological trends. We have characterized the prophages harboured by 275 human GBS isolates belonging to the major lineages. METHODS: We applied whole genome sequencing (WGS) to 14 isolates representative of the diversity within GBS species, located and identified their prophages...
September 1, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28817995/intrapartum-test-for-detection-of-group-b-streptococcus-colonization-during-labor
#14
Elena Picchiassi, Giuliana Coata, Giulia Babucci, Irene Giardina, Valentina Summa, Federica Tarquini, Michela Centra, Vittorio Bini, Benito Cappuccini, Gian Carlo Di Renzo
PURPOSE: The purpose of this study was to evaluate the potential improvement of introducing an intrapartum test for the detection of Group B Streptococcus (GBS) during labor and to estimate its cost-effectiveness versus antepartum GBS screening culture. MATERIALS AND METHODS: Three hundred and thirteen women at beginning of labor, with unknown GBS status or with antepartum GBS screening culture were enrolled. A vaginal-rectal specimen was collected from each woman for GBS detection by real-time PCR...
August 31, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28806562/zika-virus-induced-neurological-critical-illness-in-latin-america-severe-guillain-barre-syndrome-and-encephalitis
#15
Ugarte Ubiergo Sebastián, Arenas Villamizar Angel Ricardo, Bruno C Alvarez, Angela Cubides, Angélica F Luna, Max Arroyo-Parejo, Cayri E Acuña, Agamenón V Quintero, Orlando Ch Villareal, Oscar S Pinillos, Elías Vieda, Manuel Bello, Susana Peña, Carmelo Dueñas-Castell, Gloria M V Rodriguez, Jorge L M Ranero, Rosa L M López, Sandra G Olaya, José C Vergara, Ana Tandazo, Juan P S Ospina, Igor M Leyton Soto, R A Fowler, John C Marshall
Zika virus (ZIKAV) is classically described as causing minor symptoms in adult patients, however neurologic complications have been recognized. The recent outbreak in Central and South America has resulted in serious illness in some adult patients. We report adult patients in Latin America diagnosed with ZIKAV infection admitted to Intensive Care Units (ICUs). METHODS: Multicenter, prospective case series of adult patients with laboratory diagnosis of ZIKAV in 16 ICUs in 8 countries. RESULTS: Between December 1st 2015 and April 2nd 2016, 16 ICUs in 8 countries enrolled 49 critically ill patients with diagnosis of ZIKAV infection...
August 9, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28798738/zika-virus-an-emerging-worldwide-threat
#16
Irfan A Rather, Jameel B Lone, Vivek K Bajpai, Woon K Paek, Jeongheui Lim
ZIKA virus (ZIKV) poses a severe threat to the world. Recent outbreaks of ZIKV after 2007 along with its quick transmission have made this virus a matter of international concern. The virus shows symptoms that are similar to those caused in the wake of dengue virus (DENV) and other flaviviruses, which makes it difficult to discern the viral infection. Diagnosis is further complicated as the virus cross-reacts with antibodies of other viruses. Currently, molecular diagnosis of the virus is being performed by RT-PCR and IgM-captured enzyme-linked immunosorbent assay (MAC-ELISA)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28779876/detection-of-streptococcus-agalactiae-by-immunochromatography-with-group-b-streptococcus-specific-surface-immunogenic-protein-in-pregnant-women
#17
Yoko Takayama, Hidehito Matsui, Yuzuru Adachi, Shin Nihonyanagi, Tatsuhiko Wada, Junko Mochizuki, Nobuya Unno, Hideaki Hanaki
BACKGROUND: Infection with Streptococcus agalactiae (Group B streptococcus: GBS) is a significant cause of morbidity and mortality in neonates. Screening for GBS is mainly done by culture-based methods, but a reliable result may take several days to obtain and culture is difficult to perform at institutions without a laboratory. We evaluated an immunochromatography method for rapid detection of GBS-specific surface immunogenic protein (Sip) using anti-Sip monoclonal antibodies. MATERIALS AND METHODS: A total of 377 cervical and vaginal swabs collected during weeks 35-37 of gestation were inoculated into GBS medium F and incubated...
October 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28776338/epidemiology-and-clinical-characteristics-of-zika-virus-infections-imported-into-korea-from-march-to-october-2016
#18
Doran Yoon, Seung Hwan Shin, Hee Chang Jang, Eu Suk Kim, Eun Hee Song, Song Mi Moon, So Youn Shin, Pyeong Gyun Choe, Jung Joon Sung, Eun Hwa Choi, Myoung Don Oh, Youngmee Jee, Nam Joong Kim
Zika is a re-emerging, mosquito-borne viral infection, which has been recently shown to cause microcephaly and Guillain-Barré syndrome. Since 2015 the number of infected patients has increased significantly in South America. The purpose of this study was to identify the epidemiologic and clinical characteristics of patients with Zika virus (ZIKV) infections in Korea. Patients who had visited areas of risk and tested positive in the ZIKV reverse transcriptase polymerase chain reaction (RT-PCR) in blood, urine, or saliva specimens were included...
September 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28764569/screening-for-group-b-streptococcus-gbs-at-labour-onset-using-pcr-accuracy-and-potential-impact-a-pilot-study
#19
Sandhya Ramesh Babu, Rachel McDermott, Irum Farooq, David Le Blanc, Wendy Ferguson, Naomi McCallion, Richard Drew, Maeve Eogan
This pilot study assessed the diagnostic accuracy and potential impact of a rapid PCR-based screening test for the detection of group B Streptococcus (GBS) at the onset of labour for the purpose of optimising intrapartum antibiotic prophylaxis (IAP). Vaginal and rectal swabs from a convenience sample of 158 women were analysed by conventional broth-enriched culture and a rapid PCR test. Overall, GBS carriage was 18.98% by culture and 19.62% by PCR. PCR for the detection of GBS had a sensitivity of 93.1%, specificity of 96...
August 1, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28760136/development-of-a-high-density-linkage-map-and-mapping-of-the-three-pistil-gene-pis1-in-wheat-using-gbs-markers
#20
Zaijun Yang, Zhenyong Chen, Zhengsong Peng, Yan Yu, Mingli Liao, Shuhong Wei
BACKGROUND: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F2 mapping population (CM28 × CM28TP) was used to construct a genetic map that is of significant value...
July 31, 2017: BMC Genomics
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