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Bruna Maria Salotti de Souza, Taís Fernanda Borgonovi, Sabrina Neves Casarotti, Svetoslav Dimitrov Todorov, Ana Lúcia Barretto Penna
The objective of this study was to evaluate the probiotic properties of Lactobacillus casei and Lactobacillus fermentum strains, as well as to select novel and safe strains for future development of functional fermented products. The in vitro auto-aggregation, co-aggregation, hydrophobicity, β-galactosidase production, survival to gastrointestinal tract (GIT), and antibiotic susceptibility were evaluated. The selected strains were additionally tested by the presence of genes encoding adhesion, aggregation and colonization, virulence factors, antibiotic resistance, and biogenic amine production, followed by the evaluation of acidifying kinetic parameters in milk, and survival of the strains under simulated GIT conditions during refrigerated storage of fermented milk...
March 14, 2018: Probiotics and Antimicrobial Proteins
Zenat Z Hossain, Israt Farhana, Suhella M Tulsiani, Anowara Begum, Peter K M Jensen
Fish have been considered natural reservoirs of Vibrio cholerae , the deadly diarrheal pathogen. However, little is known about the role of fish in the transmission of V. cholerae from the Bay of Bengal to the households of rural and urban Bangladesh. This study analyzes the incidence and pathogenic potential of V. cholerae in Hilsha ( Tenualosa ilisha ), a commonly caught and consumed fish that exhibits a life cycle in both freshwater and marine environments in Bangladesh. During the period from October 2014 to October 2015, samples from the gills, recta, intestines, and scale swabs of a total of 48 fish were analyzed...
2018: Frontiers in Microbiology
Chin-Yi Chen, Terence P Strobaugh, Ly-Huong T Nguyen, Melanie Abley, Rebecca L Lindsey, Charlene R Jackson
While antimicrobial resistance in Salmonella enterica is mainly attributed to large plasmids, small plasmids may also harbor antimicrobial resistance genes. Previously, three major groups of ColE1-like plasmids conferring kanamycin-resistance (KanR) in various S. enterica serotypes from diagnostic samples of human or animals were reported. In this study, over 200 KanR S. enterica isolates from slaughter samples, collected in 2010 and 2011 as a part of the animal arm of the National Antimicrobial Resistance Monitoring System, were screened for the presence of ColE1-like plasmids...
2018: PloS One
Amal Oudghiri, Hind Karimi, Fouad Chetioui, Fathiah Zakham, Jamal Eddine Bourkadi, My Driss Elmessaoudi, Amin Laglaoui, Imane Chaoui, Mohammed El Mzibri
BACKGROUND: The emergence of extensively drug-resistant tuberculosis (XDR-TB) has raised public health concern for global TB control. Although multi drug-resistant tuberculosis (MDR- TB) prevalence and associated genetic mutations in Morocco are well documented, scarce information on XDR TB is available. Hence, the evaluation of pre-XDR and XDR prevalence, as well as the mutation status of gyrA, gyrB, rrs, tlyA genes and eis promoter region, associated with resistance to second line drugs, is of great value for better management of M/XDR TB in Morocco...
February 27, 2018: BMC Infectious Diseases
Ngoc Tuan Nguyen
Emulsifiers like polysorbate family, carboxymethyl cellulose are widely used in the preparation of drugs, vaccines, food, cosmetics, and skin care products. In this study, strain SP2 is isolated for the high-efficiency utilization of emulsifier as its sole carbon and energy source. Strain grows on the polysorbate family with generation time ranging from 0.8 to 2.2 h. It is sensitive to ampicillin, gentamicin, imipenem, penicillin, and vancomycin, but resistant to amikacin, cefoxitin, erythromycin, kanamycin, nalidixic acid, and tetracycline...
February 24, 2018: Current Microbiology
Chenyang Li, Greta Gölz, Thomas Alter, Andrea Barac, Stefan Hertwig, Carolin Riedel
Yersinia enterocolitica is a zoonotic enteropathogenic bacterium that can cause acute gastroenteritis and mesenteric lymphadenitis. Although Y. enterocolitica is common in animals, food, and the environment, the reservoirs and transmission routes of this pathogen are still not fully understood. The aim of our study was to determine the prevalence of Y. enterocolitica in seafood in Germany, because only limited data are available on that topic. Seafood samples were purchased from retail shops in Berlin, Germany and examined for the presence of Y...
February 23, 2018: Journal of Food Protection
Li Liang, Qianqian Zhou, Zhixiang Hao, Fanfan Wang, Yasheng Zhu, Qisi Lin, Jian Gao
BACKGROUND: In recent years, Staphylococcus aureus have developed resistance to medicines used for the treatment of human infections. Therefore, the search for antibacterial agents of high potency against Staphylococcus aureus is of great concern. Peptide deformylase (PDF), a metalloprotease catalyzing the removal of a formyl group from newly synthesized proteins, has been considered to be an important antibacterial drug target. OBJECTIVE: To discover novel antibacterial drugs based on Staphylococcus aureus peptide deformylase...
February 20, 2018: Combinatorial Chemistry & High Throughput Screening
Z Mike Chirenje, Nicholas Dhibi, H Hunter Handsfield, Elizabeth Gonese, Beth Tippett Barr, Lovemore Gwanzura, Ahmed S Latif, Dumisili Venessa Maseko, Ranmini S Kularatne, Mufuta Tshimanga, Peter H Kilmarx, Anna Machiha, Owen Mugurungi, Cornelis A Rietmeijer
INTRODUCTION: Symptomatic vaginal discharge is a common gynecological condition managed syndromically in most developing countries. In Zimbabwe, women presenting with symptomatic vaginal discharge are treated with empirical regimens that commonly cover both sexually transmitted infections (STI) and reproductive tract infections, typically including a combination of an intramuscular injection of kanamycin, and oral doxycycline and metronidazole regimens. This study was conducted to determine the current etiology of symptomatic vaginal discharge and assess adequacy of current syndromic management guidelines...
November 29, 2017: Sexually Transmitted Diseases
Hideki Araoka, Masaru Baba, Chikako Okada, Muneyoshi Kimura, Tomoaki Sato, Yutaka Yatomi, Kyoji Moriya, Akiko Yoneyama
Background: Previous studies suggest that Helicobacter cinaedi can cause recurrent bacteremia. This study aimed to elucidate the risk factors for recurrent H. cinaedi bacteremia and explore the efficacy of selective digestive decontamination (SDD) as a preventive measure. Methods: We retrospectively reviewed the medical records of all patients with H. cinaedi bacteremia between March 2009 and December 2016 at two Japanese hospitals. Results: We identified 168 patients with H...
February 15, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Mohammad A M Momin, Ian G Tucker, Colin S Doyle, John A Denman, Shubhra Sinha, Shyamal C Das
High dose delivery of drugs to the lung using a dry powder inhaler (DPI) is an emerging approach to combat drug-resistant local infections. To achieve this, highly aerosolizable powders are required. We hypothesized that co-spray-drying kanamycin, a hydrophilic hygroscopic antibiotic, with rifampicin, a hydrophobic antibiotic, would produce inhalable particles with surfaces enriched in rifampicin. Such particles would have higher aerosolization than kanamycin alone, and minimise the mass of powder for inhalation avoiding use of non-active excipients...
February 16, 2018: International Journal of Pharmaceutics
Hazem Ramadan, Nermin Ibrahim, Mohamed Samir, Amany Abd El-Moaty, Tamer Gad
AIMS: Aeromonas hydrophila (A. hydrophila) has been isolated from various fish species in Egypt and is known to carry virulence and antimicrobial resistance genes, which pose risk for public health. The aim of the present study is to report, for the first time, the infection of mullet (Mugil cephalus) with A. hydrophila and to clarify the potential association between antimicrobial resistance and virulence traits encoded in A. hydrophila. METHODS AND RESULTS: In this study, the occurrence of A...
February 17, 2018: Journal of Applied Microbiology
Beryl Vedha Yesudhason, Paulkumar Kanniah, Elaiya Raja Subramanian, Vasanthakumar Ponesakki, Veeraragavan Rajendiran, Sudhakar Sivasubramaniam
Both the evaluation and the determination of toxicity of chemical substances present in the environment have implications in human health. In this present study, the natural phenomenon named autotomy, a self-defense mechanism employed by several animals against the toxic chemical contaminants, was considered to assess the toxicity of different chemical substances. We investigated the effects of glucose, sodium chloride, kanamycin, mercuric chloride, arsenic trioxide, and lead oxide on the phenotypes of earthworm Eudrilus eugeniae...
February 16, 2018: Environmental Monitoring and Assessment
Zhenhui Cao, Hongbin Pan, Shijun Li, Chongying Shi, Sifan Wang, Fuyi Wang, Pengfei Ye, Junjing Jia, Changrong Ge, Qiuye Lin, Zhiyong Zhao
This study aimed to investigate the probiotic potential of lactic acid bacteria (LAB) strains isolated from De'ang pickled tea, a traditional food consumed by the De'ang nationality of Yunnan, China. Twenty-six LAB strains isolated from De'ang pickled tea were subjected to identification based on 16S rRNA gene sequence analysis. Twenty-four belonged to Lactobacillus plantarum, one belonged to Enterococcus casseliflavus, and one belonged to Lactobacillus acidophilus. Eighteen out of 26 LAB strains which showed a higher capability to tolerate simulated gastrointestinal juices were chosen to further evaluate their probiotic properties...
February 14, 2018: Probiotics and Antimicrobial Proteins
Isabelle Guay, Simon Boulanger, Charles Isabelle, Eric Brouillette, Félix Chagnon, Kamal Bouarab, Eric Marsault, François Malouin
BACKGROUND: Tomatidine (TO) is a plant steroidal alkaloid that possesses an antibacterial activity against the small colony variants (SCVs) of Staphylococcus aureus. We report here the spectrum of activity of TO against other species of the Bacillales and the improved antibacterial activity of a chemically-modified TO derivative (FC04-100) against Listeria monocytogenes and antibiotic multi-resistant S. aureus (MRSA), two notoriously difficult-to-kill microorganisms. METHODS: Bacillus and Listeria SCVs were isolated using a gentamicin selection pressure...
February 13, 2018: BMC Pharmacology & Toxicology
Neha Rajpara, Mrinalini Nair, Ashima Kushwaha Bhardwaj
In an earlier study from this laboratory, Vibrio fluvialis BD146, a clinical isolate from Kolkata, India, 2002, was found to be resistant to all the fourteen antibiotics tested. It harboured a high copy number plasmid pBD146 and a low copy number plasmid. In the present study, a more detailed analysis was carried out to unravel different resistance mechanisms in this isolate. Sequencing showed that variable region of class 1 integron located on low copy number plasmid harbored arr3-cmlA-blaOXA10-aadA1 gene cassettes...
March 2018: Indian Journal of Microbiology
Magdalena Wypij, Magdalena Świecimska, Joanna Czarnecka, Hanna Dahm, Mahendra Rai, Patrycja Golinska
AIMS: Presently, the effective antimicrobial agents have been limited by emergence of microbial strains with multi-drug resistance and biofilm formation potential. In the present study, we report remarkable antimicrobial activity of silver nanoparticles (AgNPs) synthesized from Streptomyces calidiresistens IF11 and IF17 strains, including inhibition of biofilm formation and synergistic effect of AgNPs and antibiotics against selected bacteria and yeasts. Cytotoxic effect of AgNPs on mammalian cell lines was also evaluated...
February 10, 2018: Journal of Applied Microbiology
Farhad Sharafati Chaleshtori, Mohamad Saholi, Reza Sharafati Chaleshtori
This research was aimed at investigating the antioxidant and antibacterial activity of Bunium persicum, Eucalyptus globulus, and rose water on multidrug-resistant Listeria species. The antibiotic resistance of Listeria spp obtained from seafood samples were determined by the Kirby-Bauer method. The antioxidant and antibacterial activity of the essential oils and extracts were evaluated using ferric reducing antioxidant power and microdilution methods, respectively. A total 2 samples (1.88%) were positive for Listeria spp...
January 2018: J Evid Based Integr Med
Shin Hye Kim, Gaon Jung, Sangjae Kim, Ja-Won Koo
The cell-penetrating peptide GV1001 has been investigated as an anticancer agent and recently demonstrated anti-oxidant and anti-inflammatory effects. It has shown a protective effect on a kanamycin (KM)-induced ototoxicity mouse model. In the present study, we administered GV1001 at different time points after inducing hair cell damage, and examined if it rescues hair cell loss and restores hearing. A deaf mouse model was created by intraperitoneal injection of KM and furosemide. First, to test the early temporal change of hearing and extent of hair cell damage after KM and furosemide injection, hearing and outer hair cells (OHCs) morphology were evaluated on day 1, day 2 and day 3 after injection...
2018: Frontiers in Cellular Neuroscience
Kunimoto Hotta, Shinichi Kondo
On the occasion of the 60th anniversary of the discovery (1957) of kanamycin (KM), a series of research achievements on KM and its semisynthetic derivative Arbekacin (ABK) are outlined. KM was first used clinically in 1958 and was appreciated for its remarkable curing effect on various bacterial infections, especially tuberculosis. ABK is a KM derivative rationally semisynthesized to overcome KM resistance due to enzymatic phosphorylation and acetylation. Since its approval in 1990 as an anti-MRSA drug, ABK has been and still is effectively used in chemotherapy because MRSA rarely develops high ABK-resistance...
February 5, 2018: Journal of Antibiotics
Nico Betterle, Anastasios Melis
Fusion constructs as protein overexpression vectors proved to be critical in the heterologous expression of terpene synthases in cyanobacteria. The concept was recently applied to the heterologous overexpression of the β-phellandrene synthase (β-PHLS) from plants, fused to the highly-expressed endogenous cpcB gene encoding the β-subunit of phycocyanin. Overexpressed CpcB*PHLS fusion proteins enhanced the heterologous yield of C10H16 β-phellandrene hydrocarbons production in Synechocystis. This work extended the concept of fusion constructs as protein overexpression vectors by showing that highly-expressed heterologous genes could also serve as leader sequences for protein overexpression in cyanobacteria...
February 4, 2018: ACS Synthetic Biology
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