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https://www.readbyqxmd.com/read/28228322/anti-tumour-research-of-recombinant-bcg-using-bzlf1-and-hgm-csf-fusion-genes
#1
Qing-Jie Xue, Yun-Qing Li, Chun-Qing Yang, Ting Chen, Xiu-Zhen Li, Baohua Cheng, Chun-Mei Wang
The random primer Oligo(dT)15 was used in RT-PCR to obtain cDNA from the human granulocyte macrophage colony stimulating factor (hGM-CSF) gene and the Epstein-Barr virus (EBV) gene BZLF1. Then, the sequence splicing overlap extension method was used to obtain a GCBF fusion gene containing a linker sequence that encoded the polypeptide (Gly4Ser)3. The GCBF fusion gene was inserted into pMV261, which was then transformed into competent E. coli DH5 alpha cells, and positive cells were selected based on kanamycin resistance on LB plates...
February 19, 2017: Vaccine
https://www.readbyqxmd.com/read/28225540/antituberculosis-drug-resistance-in-pulmonary-isolates-of-mycobacterium-tuberculosis-cuba-2012-2014
#2
Dihadenys Lemus, Miguel Echemendía, Raúl Díaz, María J Llanes, Lourdes Suárez, Antonio Marrero
INTRODUCTION Systematic surveillance of antituberculosis drug resistance allows identification of multidrug-resistant and extensively drug-resistant isolates of Mycobacterium tuberculosis. Surveillance studies of antituberculosis drug resistance systematically conducted in Cuba for over 15 years have revealed low circulation of multidrug-resistant tuberculosis, under 1% in new cases. OBJECTIVE Characterize antituberculosis drug resistance in isolates of M. tuberculosis recovered from patients with pulmonary tuberculosis in Cuba in 2012-2014...
January 2017: MEDICC Review
https://www.readbyqxmd.com/read/28223861/biogenic-nanoparticles-bearing-antibacterial-activity-and-their-synergistic-effect-with-broad-spectrum-antibiotics-emerging-strategy-to-combat-drug-resistant-pathogens
#3
Syed Baker, Azmath Pasha, Sreedharamurthy Satish
The present study emphasizes on synthesis of bimetallic silver-gold nanoparticles from cell free supernatant of Pseudomonas veronii strain AS41G inhabiting Annona squamosa L. The synthesized nanoparticles were characterized using hyphenated techniques with UV-Visible spectra ascertained absorbance peak between 400 and 800 nm. Possible interaction of biomolecules in mediating and stabilization of nanoparticles was depicted with Fourier transform infrared spectroscopy (FTIR). X-ray diffraction (XRD) displayed Bragg's peak conferring the 1 0 0, 1 1 1, 2 0 0, and 2 2 0 facets of the face centered cubic symmetry of nanoparticles suggesting that these nanoparticles were crystalline in nature...
January 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28210241/outer-membrane-proteins-form-specific-patterns-in-antibiotic-resistant-edwardsiella-tarda
#4
Bo Peng, Chao Wang, Hui Li, Yu-Bin Su, Jin-Zhou Ye, Man-Jun Yang, Ming Jiang, Xuan-Xian Peng
Outer membrane proteins of Gram-negative bacteria play key roles in antibiotic resistance. However, it is unknown whether outer membrane proteins that respond to antibiotics behave in a specific manner. The present study specifically investigated the differentially expressed outer membrane proteins of an antibiotic-resistant bacterium, Edwardsiella tarda, a Gram-negative pathogen that can lead to unnecessary mass medication of antimicrobials and consequently resistance development in aquaculture and a spectrum of intestinal and extraintestinal diseases in humans...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28199151/antimicrobial-resistance-percentages-of-salmonella-and-shigella-in-seafood-imported-to-jordan-higher-percentages-and-more-diverse-profiles-in-shigella
#5
Mohammad M Obaidat, Alaa E Bani Salman
This study determined the prevalence and antimicrobial resistance of human-specific ( Shigella spp.) and zoonotic ( Salmonella enterica ) foodborne pathogens in internationally traded seafood. Sixty-four Salmonella and 61 Shigella isolates were obtained from 330 imported fresh fish samples from Egypt, Yemen, and India. The pathogens were isolated on selective media, confirmed by PCR, and tested for antimicrobial resistance. Approximately 79 and 98% of the Salmonella and Shigella isolates, respectively, exhibited resistance to at least one antimicrobial, and 8 and 49% exhibited multidrug resistance (resistance to three or more antimicrobial classes)...
February 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28192916/combating-enhanced-intracellular-survival-eis-mediated-kanamycin-resistance-of-mycobacterium-tuberculosis-by-novel-pyrrolo-1-5-a-pyrazine-based-eis-inhibitors
#6
Atefeh Garzan, Melisa J Willby, Huy X Ngo, Chathurada S Gajadeera, Keith D Green, Selina Y L Holbrook, Caixia Hou, James E Posey, Oleg V Tsodikov, Sylvie Garneau-Tsodikova
Tuberculosis (TB) remains one of the leading causes of mortality worldwide. Hence, the identification of highly effective antitubercular drugs with novel modes of action is crucial. In this paper, we report the discovery and development of pyrrolo[1,5-a]pyrazine-based analogues as highly potent inhibitors of the Mycobacterium tuberculosis (Mtb) acetyltransferase enhanced intracellular survival (Eis), whose up-regulation causes clinically observed resistance to the aminoglycoside (AG) antibiotic kanamycin A (KAN)...
February 17, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28191448/a-food-grade-expression-system-for-d-psicose-3-epimerase-production-in-bacillus-subtilis-using-an-alanine-racemase-encoding-selection-marker
#7
Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun, Dawei Zhang
BACKGROUND: Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis. RESULTS: In this study, the d-alanine racemase-encoding gene dal was deleted from the chromosome of B. subtilis 1A751 using Cre/lox system to generate the food-grade host...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28190829/a-label-free-and-turn-on-fluorescence-strategy-for-kanamycin-detection-based-on-the-nmm-g-quadruplex-structure
#8
Hualin Yang, Qinghua Wu, Dongxiao Su, Yun Wang, Li Li, Xingping Zhang
In the present work, a simple design of a "label-free" fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28182844/characterization-of-antibiotic-resistance-genes-from-lactobacillus-isolated-from-traditional-dairy-products
#9
Huiling Guo, Lin Pan, Lina Li, Jie Lu, Laiyu Kwok, Bilige Menghe, Heping Zhang, Wenyi Zhang
Lactobacilli are widely used as starter cultures or probiotics in yoghurt, cheese, beer, wine, pickles, preserved food, and silage. They are generally recognized as safe (GRAS). However, recent studies have shown that some lactic acid bacteria (LAB) strains carry antibiotic resistance genes and are resistant to antibiotics. Some of them may even transfer their intrinsic antibiotic resistance genes to other LAB or pathogens via horizontal gene transfer, thus threatening human health. A total of 33 Lactobacillus strains was isolated from fermented milk collected from different areas of China...
February 9, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28168547/resonance-light-scattering-method-for-detecting-kanamycin-in-milk-with-enhanced-sensitivity
#10
Chengke Wang, Chengen Wang, Qingqing Wang, Dan Chen
In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would aggregate in the presence of NaCl, which resulted in the RLS signal intensities enhanced dramatically. Based on this phenomenon, kanamycin can be detected in the range of 10 to 600 nM with a limit of detection as 1 nM, which is more sensitive than many other instrumental methods, especially the commonly used UV-visible spectroscopic method...
February 6, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28161846/genotypic-analysis-of-virulence-genes-and-antimicrobial-profile-of-diarrheagenic-escherichia-coli-isolated-from-diseased-lambs-in-iran
#11
Reza Ghanbarpour, Nasrin Askari, Masoud Ghorbanpour, Yahya Tahamtan, Khoobyar Mashayekhi, Narjes Afsharipour, Nasim Darijani
The aim of the present study was to determine the analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs. Two hundred ninety E. coli isolates were recovered from 300 rectal swabs of diarrheic lambs and were confirmed by biochemical tests. The pathotype determination was done according to the presence of genes including f5, f41, LTI, STI, bfp, ipaH, stx 1 , stx 2 , eae, ehlyA, cnf 1 , cnf 2 , cdIII, cdIV, and f17 by PCR method. Sixty-six isolates (23...
February 4, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28160653/monitoring-of-microbial-cell-viability-using-nanostructured-electrodes-modified-with-graphene-alumina-nanocomposite
#12
Rabeay Y A Hassan, Moataz M Mekawy, Pankaj Ramnani, Ashok Mulchandani
Microbial infections are rapidly increasing; however most of the existing microbiological and molecular detection methods are time consuming and/or cannot differentiate between the viable and dead cells which may overestimate the risk of infections. Therefore, a bioelectrochemical sensing platform with a high potential to the microbial-electrode interactions was designed based on decorated graphene oxide (GO) sheet with alumina (Al2O3) nanocrystals. GO-Al2O3 nanocomposite was synthesized using self-assembly of GO and Al2O3 and characterized using the scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), Raman-spectroscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV)...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28153888/deep-sequencing-identified-kanamycin-resistant-paenibacillus-sp-strain-ks1-isolated-from-epiphyte-tillandsia-usneoides-spanish-moss-in-central-florida-usa
#13
Pushpa Lata, Subramaniam S Govindarajan, Feng Qi, Jian-Liang Li, Malaya K Sahoo
Paenibacillus sp. strain KS1 was isolated from an epiphyte, Tillandsia usneoides (Spanish moss), in central Florida, USA. Here, we report a draft genome sequence of this strain, which consists of a total of 398 contigs spanning 6,508,195 bp, with a G+C content of 46.5% and comprising 5,401 predicted coding sequences.
February 2, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28152272/radiolabeling-quality-control-and-biological-characterization-of-177-lu-labeled-kanamycin
#14
Muhammad Usman Akbar, Tanveer Hussain Bokhari, Muhammad Khalid, Muhammad Razeen Ahmad, Samina Roohi, Saira Hina, Sajid Mehmood, Muhammad Sohaib, Tania Jabbar
Kanamycin is an antibiotic, isolated from Streptomyces kanamyceticus, which is used to treat serious bacterial infections. The fact that the present radioligand (99m) Tc-kanamycin used for diagnosis is short-lived, raised a need to label and study kanamycin with one of the most important beta (β) radiation emitting isotope (177) Lu. Labeling yield of (177) Lu-kanamycin was confirmed by different chromatography techniques such as paper chromatography, TLC, HPLC. Several experiments were performed to optimize labeling with changing reaction conditions such as pH, temperature, amount of ligand, and reaction time etc...
February 2, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28151044/dissemination-of-genes-encoding-aminoglycoside-modifying-enzymes-and-arma-among-enterobacteriaceae-isolates-in-northwest-iran
#15
Reza Ghotaslou, Fatemeh Yeganeh Sefidan, Mohammad Taghi Akhi, Mohammad Asgharzadeh, Yalda Mohammadzadeh Asl
INTRODUCTION: Enzymatic inactivation is one of the most important mechanisms of resistance to aminoglycosides. The aim of this study was to investigate the prevalence of armA and diversity of the genes encoding aminoglycoside-modifying enzymes (AMEs) and their associations with resistance phenotypes in Enterobacteriaceae isolates. METHODS: Three hundred and seven Enterobacteriaceae isolates were collected from five hospitals in northwest Iran. The disk diffusion method for amikacin, gentamicin, tobramycin, kanamycin, and streptomycin, as well as the minimum inhibitory concentration for amikacin, gentamicin, tobramycin, and kanamycin were done for susceptibility testing...
February 2, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28150571/sanguibacter-gelidistatuariae-sp-nov-a-novel-psychrotolerant-anaerobe-from-an-ice-sculpture-in-antarctica-and-emendation-of-descriptions-for-the-family-sanguibacteraceae-the-genus-sanguibacter-and-species-s-antarcticus-s-inulinus-s-kedieii-s-marinus-s-soli
#16
Elena V Pikuta, Zhe Lyu, Melissa D Williams, Nisha B Patel, Yuchen Liu, Richard Brice Hoover, Hans-Jürgen Busse, Paul A Lawson, William B Whitman
A novel psychrotolerant bacterium strain ISLP-3T was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase positive. Growth occurred at 3-35 ºC with an optimum at 24 °C, 0-2 % w/v NaCl with an optimum at 0.3 % and pH 6.2 - 9.5 with an optimum 7.5. Strain ISLP-3T grew on several carbon sources, with the best growth on D-cellobiose...
February 1, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28132897/targeting-biofilms-and-persisters-of-eskape-pathogens-with-p14kans-a-kanamycin-peptide-conjugate
#17
Mohamed F Mohamed, Anna Brezden, Haroon Mohammad, Jean Chmielewski, Mohamed N Seleem
BACKGROUND: The worldwide emergence of antibiotic resistance represents a serious medical threat. The ability of these resistant pathogens to form biofilms that are highly tolerant to antibiotics further aggravates the situation and leads to recurring infections. Thus, new therapeutic approaches that adopt novel mechanisms of action are urgently needed. To address this significant problem, we conjugated the antibiotic kanamycin with a novel antimicrobial peptide (P14LRR) to develop a kanamycin peptide conjugate (P14KanS)...
January 27, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28127196/construction-of-gpm6a-reelin-gfpcreert2-by-bac-recombination-using-a-specific-gene-in-hepatic-mesothelial-or-stellate-cells
#18
Hong-Bo Shi, Jin-Li Lou, Hong-Lin Shi, Feng Ren, Yu Chen, Zhong-Ping Duan
AIM: To prepare a Gpm6a/Reelin(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC). METHODS: Gpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion...
January 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28123141/antimicrobial-resistance-profiles-and-molecular-characterization-of-escherichia-coli-strains-isolated-from-healthy-adults-in-ho-chi-minh-city-vietnam
#19
Phuong Hoai Hoang, Sharda Prasad Awasthi, Phuc DO Nguyen, Ngan Ly Hoang Nguyen, Dao Thi Anh Nguyen, Ninh Hoang LE, Chinh VAN Dang, Atsushi Hinenoya, Shinji Yamasaki
In this study, we attempted to isolate Escherichia coli from healthy individuals in Ho Chi Minh City, Vietnam, and characterized its antimicrobial resistance profile, extended-spectrum β-lactamase (ESBL) genotype, phylogenetic grouping and virulence gene profile. A total of 103 E. coli strains were obtained, and most of them were antimicrobial resistant such to streptomycin (80.6%), tetracycline (67.0%), ampicillin (65.0%), sulfamethoxsazole/trimethoprim (48.5%), nalidixic acid (43.7%), chloramphenicol (34...
January 26, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28123098/astrobiology-as-a-framework-for-investigating-antibiotic-susceptibility-a-study-of-halomonas-hydrothermalis
#20
Jesse P Harrison, Roey Angel, Charles S Cockell
Physical and chemical boundaries for microbial multiplication on Earth are strongly influenced by interactions between environmental extremes. However, little is known about how interactions between multiple stress parameters affect the sensitivity of microorganisms to antibiotics. Here, we assessed how 12 distinct permutations of salinity, availability of an essential nutrient (iron) and atmospheric composition (aerobic or microaerobic) affect the susceptibility of a polyextremotolerant bacterium, Halomonas hydrothermalis, to ampicillin, kanamycin and ofloxacin...
January 2017: Journal of the Royal Society, Interface
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