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https://www.readbyqxmd.com/read/29786847/assessment-of-antibiotic-susceptibility-within-lactic-acid-bacteria-and-coagulase-negative-staphylococci-isolated-from-hunan-smoked-pork-a-naturally-fermented-meat-product-in-china
#1
Jing Wang, Xinyuan Wei, Mingtao Fan
The aim of this study was to evaluate the antibiotic susceptibility of lactic acid bacteria (LAB) and coagulase-negative staphylococci (CNS) strains isolated from naturally fermented smoked pork produced in Hunan, China. A total of 48 strains were isolated by selective medium and identified at the species level by 16S rRNA gene sequencing as follows: Staphylococcus carnosus (23), Lactobacillus plantarum (12), Lactobacillus brevis (10), Lactobacillus sakei (1), Weissella confusa (1), and Weissella cibaria (1)...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29783082/multiplexed-aptasensor-based-on-metal-ions-labels-for-simultaneous-detection-of-multiple-antibiotic-residues-in-milk
#2
Falan Li, Yemin Guo, Xiangyou Wang, Xia Sun
A dual-target electrochemical aptasensor was developed for the simultaneous detection of multiple antibiotics based on metal ions as signal tracers and nanocomposites as signal amplification strategy. Metal ions such as Cd2+ and Pb2+ could generate distinct differential pulse voltammetry (DPV) peaks. When targets were present, kanamycin (KAN) and streptomycin (STR) as models, the KAN aptamer (KAP) and STR aptamer (STP) were released from their complementary strands, with more change of Cd2+ and Pb2+ corresponding to peak currents...
April 13, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29781496/a-universal-platform-for-selection-and-high-resolution-phenotypic-screening-of-bacterial-mutants-using-the-nanowell-slide
#3
H Antypas, M Veses-Garcia, E Weibull, H Andersson-Svahn, A Richter-Dahlfors
The Petri dish and microtiter plate are the golden standard for selection and screening of bacteria in microbiological research. To improve on the limited resolution and throughput of these methods, we developed a universal, user-friendly platform for selection and high-resolution phenotypic screening based on the nanowell slide. This miniaturized platform has an optimal ratio between throughput and assay complexity, holding 672 nanowells of 500 nl each. As monoclonality is essential in bacterial genetics, we used FACS to inoculate each nanowell with a single bacterium in 15 min...
May 21, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29781236/detection-of-acetyltransferase-modification-of-kanamycin-an-aminoglycoside-antibiotic-in-bacteria-using-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#4
Johnny J Perez, Chin-Yi Chen
RATIONALE: The occurrence of antibiotic resistant (AR) bacteria is a worldwide issue that has the potential, if not addressed, to eliminate classes of antibiotics that have extended life expectancy in the last century. An approach to confront this threat is the development of technologies that greatly accelerate the detection of AR to minimize unnecessary treatment involving antibiotics. Development of an analytical method for rapid detection of aminoglycoside resistance using liquid chromatography mass spectrometry (LC-MS) has not been reported in literature and is described here...
May 20, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29779781/tag-array-gene-chip-rapid-diagnosis-anti-tuberculosis-drug-resistance-in-pulmonary-tuberculosis-a-feasibility-study
#5
Wenjie Wu, Peng Cheng, Jingtong Lyu, Zehua Zhang, Jianzhong Xu
OBJECTIVE: We developed a Tag Array chip for detecting first- and second-line anti tuberculosis drug resistance in pulmonary tuberculosis and compared the analytical performance of the gene chip to that of phenotypic drug susceptibility testing (DST). METHODS: From November 2011 to April 2016.234 consecutive culture-confirmed, clinically and imaging diagnosed patients with pulmonary tuberculosis from Southwest Hospital, Chongqing were enrolled into the study. Specimens collected during sputum or bronchoalveolar lavage fluid from the pulmonary tuberculosis patients were subjected to M...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29779779/evaluation-of-pyrosequencing-for-extensive-drug-resistance-defining-anti-tuberculosis-drugs-for-use-in-public-healthcare
#6
Remya Nambiar, Daksha Shah, Kanchan Ajbani, Mubin Kazi, Meeta Sadani, Anjali Shetty, Padmaja Keskar, Sanjeev Kamble, Alex van Belkum, Camilla Rodrigues
MGIT 960 drug susceptibility testing (DST) for Mycobacterium tuberculosis was compared for performance and speed with pyrosequencing (PSQ). Pulmonary samples (n = 100), from GeneXpert/MTB/Rifampicin-resistant patients receiving second-line treatment for 1-3 months, were subjected to DST and PSQ for seven drugs (isoniazid, rifampicin, kanamycin, amikacin, capreomycin, moxifloxacin, and ofloxacin). The mean time-to-result was 35 and two days for DST and PSQ, respectively. Average concordancy was 92.7%. Theoretically, PSQ showed substantial incremental value over the commercial Genotype MTBDRplus/sl...
May 2018: Tuberculosis
https://www.readbyqxmd.com/read/29772394/antimicrobial-resistance-and-genetic-lineages-of-faecal-enterococci-of-wild-birds-emergence-of-vana-and-vanb2-harboring-enterococcus-faecalis
#7
Houssem Ben Yahia, Sarra Chairat, Nabil Hamdi, Haythem Gharsa, Rym Ben Sallem, Sara Ceballos, Carmen Torres, Karim Ben Slama
Migrating birds have been implicated in pathogen dissemination over long-distances. The lack of data on birds' intestinal microbiota makes these animals a promising path to understand their potential role in the transmission of antibiotic resistant bacteria. This work aims to study the diversity of enterococci species and to analyze the antimicrobial resistant phenotypes/genotypes, as well as the genetic lineages of isolates obtained from faecal and pellet samples of colonial wild birds in Tunisia. Seventy-nine enterococci were recovered of 150 wild bird, after inoculation of samples in Slanetz-Bartley agar, and were identified as E...
May 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29766599/randomized-clinical-trial-to-evaluate-the-effectiveness-of-enrofloxacin-as-a-second-line-antibiotic-for-treatment-of-acute-escherichia-coli-mastitis
#8
Yasunori Shinozuka, Kazuhiro Kawai, Akira Takeda, Michiaki Yamada, Fumi Kayasaki, Nao Kondo, Yoshiko Sasaki, Nahoko Kanai, Takuma Mukai, Naoki Sawaguchi, Masaya Higuchi, Haruka Kondo, Kouki Sugimoto, Akane Yasuda, Aiko Watanabe
The objective of the present study was to evaluate the effectiveness of enrofloxacin (ERFX) as a second-line antibiotic for treatment of acute Escherichia coli (E. coli) mastitis. Forty-two cows with naturally occurring acute E. coli mastitis were enrolled. On the first day of treatment (day 0), empirically selected antibiotics (oxytetracycline: n = 32, kanamycin: n = 10) were administered. Although systemic signs improved in 10 cows (first-line group), the signs remained unchanged or worsened in 32 cows on day 1, including two cows that were found dead...
May 16, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29765621/biofilm-inhibition-and-pathogenicity-attenuation-in-bacteria-by-proteus-mirabilis
#9
Shichen Yu, Xiaoshan Zhu, Jin Zhou, Zhonghua Cai
Biofilms play an important role in the antibiotic resistance of encased bacteria, and biofilm formation is regulated by quorum sensing (QS). Inhibiting the QS system may, therefore, degrade the integrity of a biofilm and expose the bacterial pathogens within it to the deleterious effects of molecules such as antibiotics. Moreover, the use of QS inhibitors (QSIs) may provide a novel approach for treating bacterial infections of aquacultures. In the present study, the bacterium Proteus mirabilis was identified as a potential producer of QSIs...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29765275/-tristemma-hirtum-and-five-other-cameroonian-edible-plants-with-weak-or-no-antibacterial-effects-modulate-the-activities-of-antibiotics-against-gram-negative-multidrug-resistant-phenotypes
#10
Gaëlle S Nguenang, Armelle T Mbaveng, Aimé G Fankam, Hermione T Manekeng, Paul Nayim, Brice E N Wamba, Victor Kuete
In order to contribute to the fight against infectious diseases, the in vitro antibacterial activity and the antibiotic-potentiating effects of Tristemma hirtum and five other Cameroonian edible plants have been evaluated against Gram-negative multidrug-resistant (MDR) phenotypes. The microdilution method was used to evaluate the bacterial susceptibility of the extracts and their combination to common antibiotics. The phytochemical screening of the extracts was carried out according to standard methods. Phytochemical analysis of the extracts revealed the presence of alkaloids, triterpenes, steroids, and polyphenols, including flavonoids in most of the tested extracts...
2018: TheScientificWorldJournal
https://www.readbyqxmd.com/read/29760139/clpa-and-htpx-proteases-are-involved-in-intrinsic-aminoglycoside-resistance-of-stenotrophomonas-maltophilia-and-are-potential-aminoglycoside-adjuvant-targets
#11
Hsin-Hui Huang, Yi-Tsung Lin, Peng-Ying Chen, Li-Hua Li, Hsiao-Chen Ning, Tsuey-Ching Yang
The linkage of protease-chaperon system, SmeYZ pump, and aminoglycoside resistance was assessed in Stenotrophomonas maltophilia The clpA , clpS , clpP , and htpX genes were upregulated in response to kanamycin exposure. Of them, clpA and htpX were the primary determinants responsible for intrinsic AG resistance. Inactivation of clpA and htpX compromised protease-mediated intrinsic aminoglycoside resistances and weakened SmeYZ pump-mediated aminoglycoside resistance, signifying HtpX and ClpA as potential AG adjuvant targets for treatment of S...
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29755727/antimicrobial-resistance-profile-of-e-coli-isolated-from-raw-cow-milk-and-fresh-fruit-juice-in-mekelle-tigray-ethiopia
#12
Haftay Abraha Tadesse, Netsenet Berhe Gidey, Kidane Workelule, Hagos Hailu, Seyfe Gidey, Abrha Bsrat, Habtamu Taddele
Aim: Foodborne illnesses represent a public health problem in developed and developing countries. They cause great suffering and are transmitted directly or indirectly between animals and humans and circulate in the global environment . E. coli are among them, causing a major public health problem. The aim of this study was therefore to study the antimicrobial resistance profile of E. coli from raw cow milk and fruit juice. Materials and Methods: A cross-sectional study was conducted from October 2016 to June 2017 on 258 samples collected from milk shops ( n = 86), dairy farms ( n = 86), and fruit juice ( n = 86) in different subcities of Mekelle...
2018: Veterinary Medicine International
https://www.readbyqxmd.com/read/29753025/identification-and-efficacy-of-glycine-serine-and-threonine-metabolism-in-potentiating-kanamycin-mediated-killing-of-edwardsiella-piscicida
#13
Jin-Zhou Ye, Xiang-Min Lin, Zhi-Xue Cheng, Yu-Bin Su, Wan-Xin Li, Far-Man Ali, Jun Zheng, Bo Peng
We previously showed that glucose potentiated kanamycin to kill multidrug-resistant Edwardsiella piscicida through activation of the TCA cycle. However, whether other regulatory mechanism is involved requires further investigation. By quantitative proteomics technology, iTRAQ, we systematically mapped the altered proteins in the presence of glucose and identified 94 differentially expressed proteins. The analysis of the altered proteins by pathways, amino acid biosynthesis and metabolism were enriched. And the most significantly altered eight amino acids tyrosine, phenylalanine, valine, leucine, isoleucine, glycine, serine and threonine were investigated for their potentiation of kanamycin to kill EIB202, where glycine, serine and threonine showed the strongest efficacy than the others...
May 9, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29741928/comparative-study-on-antibiotic-resistance-and-dna-profiles-of-salmonella-enterica-serovar-typhimurium-isolated-from-humans-retail-foods-and-the-environment-in-shanghai-china
#14
Zengfeng Zhang, Chenyang Cao, Bin Liu, Xuebin Xu, Yanfei Yan, Shenghui Cui, Sheng Chen, Jianghong Meng, Baowei Yang
We characterized antibiotic resistance profiles, antibiotic resistance-associated genes, and pulsed-field gel electrophoresis (PFGE) patterns of 145 Salmonella enterica serotype Typhimurium isolates from human infections and retail foods that were possibly responsible for salmonellosis outbreaks from 2008 to 2012 in Shanghai, China. Resistance to at least three antibiotics was found in 66.7% of chicken isolates, 76.5% of duck isolates, 77.8% of pork isolates, and 80.5% of human isolates. Seven antibiotic resistance phenotypes were detected in chicken isolates, 16 in pork isolates, 17 in duck isolates, and 50 in human isolates...
May 9, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29732452/katg-mediated-oxidation-leading-to-reduced-susceptibility-of-bacteria-to-kanamycin
#15
Peter C Loewen, P Malaka De Silva, Lynda J Donald, Jacek Switala, Jacylyn Villanueva, Ignacio Fita, Ayush Kumar
Resistance to antibiotics has become a serious problem for society, and there are increasing efforts to understand the reasons for and sources of resistance. Bacterial-encoded enzymes and transport systems, both innate and acquired, are the most frequent culprits for the development of resistance, although in Mycobacterium tuberculosis , the catalase-peroxidase, KatG, has been linked to the activation of the antitubercular drug isoniazid. While investigating a possible link between aminoglycoside antibiotics and the induction of oxidative bursts, we observed that KatG reduces susceptibility to aminoglycosides...
April 30, 2018: ACS Omega
https://www.readbyqxmd.com/read/29731246/gdf11-induces-kidney-fibrosis-renal-cell-epithelial-to-mesenchymal-transition-and-kidney-dysfunction-and-failure
#16
Marianne Pons, Leonidas G Koniaris, Sharon M Moe, Juan C Gutierrez, Aurora Esquela-Kerscher, Teresa A Zimmers
BACKGROUND: GDF11 modulates embryonic patterning and kidney organogenesis. Herein, we sought to define GDF11 function in the adult kidney and in renal diseases. METHODS: In vitro renal cell lines, genetic, and murine in vivo renal injury models were examined. RESULTS: Among tissues tested, Gdf11 was highest in normal adult mouse kidney. Expression was increased acutely after 5/6 nephrectomy, ischemia-reperfusion injury, kanamycin toxicity, or unilateral ureteric obstruction...
May 3, 2018: Surgery
https://www.readbyqxmd.com/read/29727265/prevalence-of-aminoglycoside-modifying-enzymes-in-escherichia-coli-and-klebsiella-pneumoniae-producing-extended-spectrum-%C3%AE-lactamases-collected-in-two-multicenter-studies-in-spain
#17
Marta Fernández-Martínez, Belén Ruiz Del Castillo, Maria Jesús Lecea-Cuello, Jesús Rodríguez-Baño, Álvaro Pascual, Luis Martínez-Martínez
The in vitro activity of amikacin, gentamicin, kanamycin, tobramycin, neomycin, and netilmicin against 420 Escherichia coli producing extended spectrum β-lactamases (Ec-ESBLs) and 139 Klebsiella pneumoniae producing extended spectrum β-lactamase (Kp-ESBL) collected in two multicenter studies performed in Spain in 2000 and 2006 was determined. The presence of genes encoding aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (rRNA) methylases [aac(3)-Ia, aac(3)-IIa, aac(3)-IVa, aac(6')-Ib, ant(2")-Ia, ant(4')-IIa, aph(3')-Ia, aph(3')-IIa, armA, rmtB, and rmtC] was also investigated...
May 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29715487/potent-insect-gut-binding-lectin-from-sclerotium-rolfsii-impart-resistance-to-sucking-and-chewing-type-insects-in-cotton
#18
Gulamnabi L Vanti, Ishwarappa S Katageri, Shashikala R Inamdar, Vamadevaiah Hiremathada, Bale M Swamy
Cotton is an important crop that is continuously cultivated around the world. However, its production has decreased in recent times due to wide ranging insects and also current practices of using synthetic insecticides that are not precise and their residues impairing the biodiversity. Hence, the search for newer classes of efficient entomotoxic proteins continues. Genetically modified cotton crops with cry genes from Bacillus thuringiensis, have been cultivated across the world, which overcome the chewing type insect menace...
April 28, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29709830/enzyme-and-label-free-electrochemical-aptasensor-for-kanamycin-detection-based-on-double-stir-bar-assisted-toehold-mediated-strand-displacement-reaction-for-dual-signal-amplification
#19
Feng Hong, Xixue Chen, Yuting Cao, Youren Dong, Dazhen Wu, Futao Hu, Ning Gan
It is critically important to detect antibiotic residues for monitoring food safety. In this study, an enzyme- and label-free electrochemical aptasensor for antibiotics, with kanamycin (Kana) as a typical analyte, was developed based on a double stir bar-assisted toehold-mediated strand displacement reaction (dSB-TMSDR) for dual-signal amplification. First, we modified two gold electrodes (E-1 and E-2) with different DNA probes (S1/S2 hybrid probe in E-1 and DNA fuel strand S3 in E-2). In the presence of Kana, an S1/S2 probe can be disassembled from E-1 to form an S2/Kana complex in supernatant...
April 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29708846/distribution-of-aminoglycoside-modifying-enzymes-and-molecular-analysis-of-the-coagulase-gene-in-clinical-isolates-of-methicillin-resistant-and-methicillin-susceptible-staphylococcus-aureus
#20
Farhad Seyedi-Marghaki, Davood Kalantar-Neyestanaki, Fereshteh Saffari, Hossein Hosseini-Nave, Mohammad Moradi
Enzymatic alteration of aminoglycosides by aminoglycoside-modifying enzymes (AMEs) is the major mechanism of resistance to aminoglycosides. The purpose of this study was to determine the frequency of AME genes, staphylococcal chromosomal cassette mec (SCCmec) types, and molecular analysis of the coagulase (coa) gene in Staphylococcus aureus strains isolated from clinical specimens. Totally, 102 S. aureus were tested by disk diffusion and microbroth dilution methods for susceptibility to aminoglycosides. AMEs genes and SCCmec types were determined by multiplex polymerase chain reaction (PCR)...
April 30, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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