Silvia Madritsch, Vivienne Arnold, Martha Haider, Julia Bosenge, Mateja Pfeifer, Beatrix Weil, Manuela Zechmeister, Markus Hengstschläger, Jürgen Neesen, Franco Laccone
PURPOSE: Various screening techniques have been developed for preimplantation genetic testing for aneuploidy (PGT-A) to reduce implantation failure and miscarriages in women undergoing in vitro fertilisation (IVF) treatment. Among these methods, the Oxford nanopore technology (ONT) has already been tested in several tissues. However, no studies have applied ONT to polar bodies, a cellular material that is less restrictively regulated for PGT-A in some countries. METHODS: We performed rapid short nanopore sequencing on pooled first and second polar bodies of 102 oocytes from women undergoing IVF treatment to screen for aneuploidy...
April 20, 2024: Journal of Assisted Reproduction and Genetics