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https://www.readbyqxmd.com/read/29144842/a-novel-fiber-chimeric-conditionally-replicative-adenovirus-ad5-f35-for-tumor-therapy
#1
Ming Yang, Chun Sheng Yang, WenWen Guo, JianQin Tang, Qian Huang, ShouXin Feng, AiJun Jiang, XiFeng Xu, Guan Jiang, Yan Qun Liu
Significant progress has been made in the diagnosis and treatment of cancer; however, significant challenges remain. Conditionally replicating adenoviruses (CRAds), which not only kill cancer cells, but also serve as vectors to express therapeutic genes, are a novel and effective method to treat cancer. However, most adenoviruses are Ad5, which infect cells through the coxsackie and adenovirus receptor (CAR). The transduction efficacy of Ad5 is restricted because of the absent or low expression of CAR on several cancer cells...
November 16, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29076943/computational-parametric-study-of-the-axial-and-radial-clearances-in-a-centrifugal-rotary-blood-pump
#2
Mohammad Amin Rezaienia, Gordon Paul, Eldad Avital, Martin T Rothman, Theodosios Korakianitis
In centrifugal rotary blood pumps (RBP), clearances are a critical parameter in determining blood trauma. This study investigates the effect of axial clearance (Cax) and radial clearance (Crad) on the hydrodynamic and hemolytic performance of a centrifugal RBP. A centrifugal pump was parameterized so that it could be defined by geometric variables Cax and Crad. Optimal Latin hypercube sampling was used to determine design points based on Cax, Crad, and rotor speed (ω). For each design point, a computational simulation was conducted to determine efficiency (η) and normalized index of hemolysis (NIH)...
October 23, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28792501/a-3-dimensional-mathematical-model-of-microbial-proliferation-that-generates-the-characteristic-cumulative-relative-abundance-distributions-in-gut-microbiomes
#3
Lena Takayasu, Wataru Suda, Eiichiro Watanabe, Shinji Fukuda, Kageyasu Takanashi, Hiroshi Ohno, Misako Takayasu, Hideki Takayasu, Masahira Hattori
The gut microbiome is highly variable among individuals, largely due to differences in host lifestyle and physiology. However, little is known about the underlying processes or rules that shape the complex microbial community. In this paper, we show that the cumulative relative abundance distribution (CRAD) of microbial species can be approximated by a power law function, and found that the power exponent of CRADs generated from 16S rRNA gene and metagenomic data for normal gut microbiomes of humans and mice was similar consistently with ∼0...
2017: PloS One
https://www.readbyqxmd.com/read/28789701/a-novel-bladder-cancer-specific-oncolytic-adenovirus-by-cd46-and-its-effect-combined-with-cisplatin-against-cancer-cells-of-car-negative-expression
#4
Wenjuan Cao, Junqiang Tian, Chong Li, Yanjun Gao, Xingchen Liu, Jianzhong Lu, Yuhan Wang, Zhiping Wang, Robert S Svatek, Ronald Rodriguez
BACKGROUND: Conditionally replicative oncolytic adenoviruses (CRAds) display significant anti-tumor effects. However, the traditional adenovirus of serotype 5 (Ad5) entering cancer cells via coxsackie virus and adenovirus receptor (CAR) can't be utilized for bladder cancer with low expression of CAR, which limits the application of Ad5. METHODS: We utilized Ad5/F11p containing the chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains to construct bladder cancer-specific chimeric type viruses Ad5/F11p-PSCAE-UPII-E1A, which can infect bladder cancer cells mediated by CD46 molecule...
August 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28717395/brain-imaging-before-primary-lung-cancer-resection-a-controversial-topic
#5
Zoe Hudson, Eveline Internullo, Anthony Edey, Isabel Laurence, Davide Bianchi, Alfredo Addeo
OBJECTIVE: International and national recommendations for brain imaging in patients planned to undergo potentially curative resection of non-small-cell lung cancer (NSCLC) are variably implemented throughout the United Kingdom [Hudson BJ, Crawford MB, and Curtin J et al (2015) Brain imaging in lung cancer patients without symptoms of brain metastases: a national survey of current practice in EnglandClin Radiol https://doi.org/10.1016/j.crad.2015.02.007]. However, the recommendations are not based on high-quality evidence and do not take into account cost implications and local resources...
2017: Ecancermedicalscience
https://www.readbyqxmd.com/read/28651743/the-combination-of-nk-and-cd8-t-cells-with-ccl20-il15-armed-oncolytic-adenoviruses-enhances-the-growth-suppression-of-tert-positive-tumor-cells
#6
Jun-Feng Ye, Wen-Xi Qi, Ming-Yuan Liu, Yang Li
Adoptive immunotherapy and targeted gene therapy have been extensively used to eliminate tumor cells. The combination treatment is capable of efficiently generating an effective antitumor immune response and disrupting tumor-induced tolerance. Moreover, effective antitumor immune responses are dependent on coordinate interaction among various effector cells. This study focused on whether the combination of cytotoxic effector cell-based adoptive immunotherapy and CCL20/IL15-armed oncolytic adenoviruses could induce enhanced antitumor activity...
August 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28638457/upregulation-of-coxsackie-adenovirus-receptor-sensitizes-cisplatin-resistant-lung-cancer-cells-to-crad-induced-inhibition
#7
Ali Sakhawat, Yanan Liu, Ling Ma, Tahir Muhammad, Shensen Wang, Lina Zhang, Xianling Cong, Yinghui Huang
Objective. Conditionally replicating adenoviruses (CRAds) have been proven potent oncolytic viruses in previous studies. They selectively replicate in the tumor cells because of incorporated survivin promoter and ultimately lead to their killing with minimal side effects on normal tissue. Chemotherapy with cisplatin is commonly employed for treating tumors, but its cytotoxic effects and development of resistance remained major concerns to be dealt with. The aim of this study was to explore the anticancer potential of survivin regulated CRAd alone or in combination with cisplatin in the A549 lung cancer cell line and cisplatin-resistant lung cancer cell line, A549-DDPR...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28279737/targeting-tumors-with-cyclic-rgd-conjugated-lipid-nanoparticles-loaded-with-an-ir780-nir-dye-in-vitro-and-in-vivo-evaluation
#8
Jungyoon Choi, Emilie Rustique, Maxime Henry, Mélanie Guidetti, Véronique Josserand, Lucie Sancey, Jérôme Boutet, Jean-Luc Coll
Like several 50nm-large nanocarriers, lipid nanoparticles (LNPs) can passively accumulate in tumors through the Enhanced Permeability and Retention (EPR) effect. In this study, we developed PEGylated LNPs loaded with IR780 iodide as a contrast agent for NIR fluorescence imaging and modified them with cyclic RGD peptides in order to target integrin avβ3. We demonstrate a specific targeting of the receptor with cRGD-LNPs but not with cRAD-LNP and standard LNP using HEK293(β3), HEK293(β3)-αvRFP, DU145 and PC3 cell lines...
November 5, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27783867/vascular-endothelial-growth-factor-promoter-based-conditionally-replicative-adenoviruses-effectively-suppress-growth-of-malignant-pleural-mesothelioma
#9
Akiko Harada, Junji Uchino, Taishi Harada, Noriaki Nakagaki, Junko Hisasue, Masaki Fujita, Koichi Takayama
Malignant mesothelioma (MM) incidence is increasing drastically worldwide as an occupational disease resulting from asbestos exposure. However, no curative treatment for MM of advanced stage is available. Thus, new therapeutic approaches for MM are required. Because malignant pleural mesothelioma (MPM) cells spread along the pleural surface in most patients, MPM can be targeted using intrapleural therapeutic approaches. In this study, we investigated the effectiveness of the intrapleural instillation of a replication-competent adenovirus as an oncolytic agent against MPM...
January 2017: Cancer Science
https://www.readbyqxmd.com/read/27633115/mechanism-of-tacrolimus-induced-chronic-renal-fibrosis-following-transplantation-is-regulated-by-ox-ldl-and-its-receptor-lox-1
#10
Shi Deng, Tao Jin, Li Zhang, Hong Bu, Peng Zhang
Chronic renal allograft dysfunction (CRAD) is the most common cause of graft failure following renal transplantation. However, the underlying mechanisms remain to be fully elucidated. Immunosuppressants and hyperlipidemia are associated with renal fibrosis following long‑term use. The present study aimed to determine the effects of tacrolimus (FK506) and lipid metabolism disorder on CRAD. In vitro and in vivo models were used for this investigation. Cells of the mouse proximal renal tubular epithelial cell strain, NRK‑52E, were cultured either with oxidized low‑density lipoprotein (ox‑LDL), FK506, ox‑LDL combined with FK506, or vehicle, respectively...
November 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27569940/expression-and-significance-of-rantes-and-mcp-1-in-renal-tissue-with-chronic-renal-allograft-dysfunction
#11
Q Yan, H Jiang, B Wang, W Sui, H Zhou, G Zou
BACKGROUND: To investigate the expression of RANTES (regulated upon activation, normal T-cell-expressed and -secreted) and monocyte chemoattractant protein-1 (MCP-1) in renal allografts with chronic renal allograft dysfunction (CRAD), and explore its relationship with interstitial fibrosis and tubular atrophy (IF/TA). METHODS: An immunohistochemical assay and computer-assisted, genuine colored image analysis system were used to detect the expression of RANTES and MCP-1 in renal allografts with CRAD...
July 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27557721/application-of-conditionally-replicating-adenoviruses-in-tumor-early-diagnosis-technology-gene-radiation-therapy-and-chemotherapy
#12
REVIEW
Shun Li, Mengting Ou, Guixue Wang, Liling Tang
Conditionally replicating adenoviruses (CRAds), or known as replication-selective adenoviruses, were discovered as oncolytic gene vectors several years ago. They have a strong ability of scavenging tumor and lesser toxicity to normal tissue. CRAds not only have a tumor-killing ability but also can combine with gene therapy, radiotherapy, and chemotherapy to induce tumor cell apoptosis. In this paper, we review the structure of CRAds and CRAd vectors and summarize the current application of CRAds in tumor detection as well as in radiotherapy and suicide gene-mediating chemotherapy...
October 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27450327/mesenchymal-stem-cells-deliver-and-release-conditionally-replicative-adenovirus-depending-on-hepatic-differentiation-to-eliminate-hepatocellular-carcinoma-cells-specifically
#13
Xiangfei Yuan, Qing Zhang, Zhenzhen Li, Xiaolong Zhang, Shiqi Bao, Dongmei Fan, Yongxin Ru, Shuxu Dong, Yizhi Zhang, Yanjun Zhang, Zhou Ye, Dongsheng Xiong
Currently, it is a key challenge to remove the postsurgical residuals and metastasis of hepatocellular carcinoma (HCC). Oncolytic adenoviral virotherapy is an attractive treatment modality for cancer; however, the difficulty remains regarding its intravenous administration. The aim of this study was to develop a targeted therapeutic system which has great potential to overcome the postsurgical residuals and metastasis of HCC. In this system, we developed a conditionally replicative adenovirus (CRAd) loaded on human umbilical cord-derived mesenchymal stem cells (HUMSCs), in which the CRAd contained an adenovirus E1A gene dual regulated by α-fetoprotein promoter and microRNA-122 target sequence...
October 10, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27380620/immunotherapeutic-effects-of-cytokine-induced-killer-cells-combined-with-ccl21-il15-armed-oncolytic-adenovirus-in-tert-positive-tumor-cells
#14
Jun-Feng Ye, Yuan-Qiang Lin, Xiu-Hua Yu, Ming-Yuan Liu, Yang Li
The effective antitumor immune responses are dependent on coordinate interaction of various effector cells. Thus, the combination of adoptive immunotherapy and target gene therapy is capable of efficiently generating a productive antitumor immune response. We investigated whether combination of cytokine-induced killer (CIK) cells adoptive immunotherapy and CCL21/IL15 armed oncolytic adenovirus could induce the enhanced antitumor activity. The CCL21/IL15 co-expression oncolytic adenoviruses were constructed by using the AdEasy system, which uses homologous recombination with shuttle plasmids and full length Ad backbones...
September 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27349517/combinatorial-strategies-based-on-crad-il24-and-crad-ing4-virotherapy-with-anti-angiogenesis-treatment-for-ovarian-cancer
#15
Ahmad Mohammad Ashshi, Adel Galal El-Shemi, Igor P Dmitriev, Elena A Kashentseva, David T Curiel
BACKGROUND: A major hurdle incurrent to the human clinical application of conditionally replicative adenovirus (CRAd)-based virotherapy agents is their limited therapeutic efficacy. In this study we evaluated whether arming our previously reported Ad5/3Δ24 CRAd vector containing a 24-base pair deletion in the E1A conserved region 2, which allows selective replication within Rb-p16-deficient tumor cells, to express therapeutic genes could improve oncolytic virus potency in ovarian cancer cells...
June 27, 2016: Journal of Ovarian Research
https://www.readbyqxmd.com/read/27345628/dendritic-cells-serve-as-a-trojan-horse-for-oncolytic-adenovirus-delivery-in-the-treatment-of-mouse-prostate-cancer
#16
Zhao-Lun Li, Xuan Liang, He-Cheng Li, Zi-Ming Wang, Tie Chong
AIM: Adenovirus-mediated gene therapy is a novel therapeutic approach for the treatment of cancer, in which replication of the virus itself is the anticancer method. However, the success of this novel therapy is limited due to inefficient delivery of the virus to the target sites. In this study, we used dendritic cells (DCs) as carriers for conditionally replicating adenoviruses (CRAds) in targeting prostate carcinoma (PCa). METHODS: Four types of CRAds, including Ad-PC (without PCa-specific promoter and a recombinant human tumor necrosis factor, rmhTNF, sequence), Ad-PC-rmhTNF (without PCa-specific promoter), Ad-PPC-NCS (without an rmhTNF sequence) and Ad-PPC-rmhTNF, were constructed...
August 2016: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/27184224/single-dose-glp-toxicity-and-biodistribution-study-of-a-conditionally-replicative-adenovirus-vector-crad-s-pk7-administered-by-intracerebral-injection-to-syrian-hamsters
#17
Julius Woongki Kim, Brenda Auffinger, Drew A Spencer, Jason Miska, Alan L Chang, Joshua Robert Kane, Jacob S Young, Deepak Kanojia, Jian Qiao, Jill F Mann, Lingjiao Zhang, Meijing Wu, Atique U Ahmed, Karen S Aboody, Theresa V Strong, Charles D Hébert, Maciej S Lesniak
BACKGROUND: CRAd-S-pk7 is a conditionally replicative oncolytic adenoviral vector that contains a survivin promoter and a pk7 fiber modification that confer tumor-specific transcriptional targeting and preferential replication in glioma while sparing the surrounding normal brain parenchyma. METHODS: This IND-enabling study performed under GLP conditions evaluated the toxicity and biodistribution of CRAd-S-pk7 administered as a single intracerebral dose to Syrian hamsters, a permissive model of adenoviral replication...
May 16, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27150992/-expression-and-significance-of-nlrp3-asc-and-aim2-in-patients-with-acute-leukemia
#18
Yong-Liang Zheng, Si-Si Zhong, Liu-Yan Xin, Yi-Jian Chen
OBJECTIVE: To explore the expression and significance of NLR family, pyrin domain containing 3 (NLRP3), apoptosis associated speck like protein containing a CRAD (ASC) and absent in melanoma 2 (AIM2) of patients with acute leukemia. METHODS: The petipheral blood samples of 19 patients with ALL and 41 patients with ANLL as the AL group (each 20 cases of newly diagnosed, relapsed and complete remission group) and 20 cases of non-hematologic malignancies as the control group were collected from July 2013 to July 2014 in the First Affiliated Hospital of Gannan Medical University...
April 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27086483/oncolytic-adenoviruses-targeted-to-human-papilloma-virus-positive-head-and-neck-squamous-cell-carcinomas
#19
Christopher J LaRocca, Joohee Han, Amanda O Salzwedel, Julia Davydova, Mark C Herzberg, Rajaram Gopalakrishnan, Masato Yamamoto
OBJECTIVES: In recent years, the incidence of Human Papilloma Virus (HPV)-positive head and neck squamous cell carcinomas (HNSCC) has markedly increased. Our aim was to design a novel therapeutic agent through the use of conditionally replicative adenoviruses (CRAds) that are targeted to the HPV E6 and E7 oncoproteins. METHODS: Each adenovirus included small deletion(s) in the E1a region of the genome (Δ24 or CB016) intended to allow for selective replication in HPV-positive cells...
May 2016: Oral Oncology
https://www.readbyqxmd.com/read/26980708/shrna-armed-conditionally-replicative-adenoviruses-a-promising-approach-for-cancer-therapy
#20
REVIEW
Jie Zhang, Meng Ding, Kai Xu, Lijun Mao, Junian Zheng
The small-interfering RNAs (siRNAs) have been employed to knockdown the expression of cancer-associated genes and shown some promise in cancer therapy. However, synthetic siRNA duplexes or plasmid mediated delivery of siRNAs have several problems, such as short half-life, low transfection efficiency and cytotoxicity associated with transfection. Conditionally replicating adenovirus (CRAds) as the delivery vector for short hairpin RNAs (shRNAs) could overcome these limitations and have shown augmented anti-tumor effects in experimental studies and preclinical trials...
May 17, 2016: Oncotarget
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