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Reproductive Imaging

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https://www.readbyqxmd.com/read/28794413/chemical-diplomacy-in-male-tilapia-urinary-signal-increases-sex-hormone-and-decreases-aggression
#1
João L Saraiva, Tina Keller-Costa, Peter C Hubbard, Ana Rato, Adelino V M Canário
Androgens, namely 11-ketotestosterone (11KT), have a central role in male fish reproductive physiology and are thought to be involved in both aggression and social signalling. Aggressive encounters occur frequently in social species, and fights may cause energy depletion, injury and loss of social status. Signalling for social dominance and fighting ability in an agonistic context can minimize these costs. Here, we test the hypothesis of a 'chemical diplomacy' mechanism through urinary signals that avoids aggression and evokes an androgen response in receiver males of Mozambique tilapia (Oreochromis mossambicus)...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28775257/sers-investigation-of-cancer-cells-treated-with-pdt-quantification-of-cell-survival-and-follow-up
#2
A B Veloso, J P F Longo, L A Muehlmann, B F Tollstadius, P E N Souza, R B Azevedo, P C Morais, S W da Silva
In this study Surface Enhanced Raman Spectroscopy (SERS) data recorded from mouse mammary glands cancer cells (4T1 cell line) was used to assess information regarding differences between control, death and viable cells after Photodynamic Therapy (PDT) treatment. The treatment used nanoemulsions (NE/PS) loaded with different chloroaluminumphthalocyanine (ClAlP) photosensitizer (PS) contents (5 and 10 µmol × L(-1)) and illumination (660 nm wavelength) at 10 J × cm(-2) (10 minutes). The SERS data revealed significant molecular alterations in proteins and lipids due to the PDT treatment...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28772059/optical-projection-tomography-with-a-tissue-clearing-agent-for-developmental-and-reproductive-toxicology-studies
#3
Alexander W Magsam, Michael Johnson, Thomas Villani, Mark C Pierce
BACKGROUND: Developmental and reproductive toxicology (DART) testing represents an expensive and time-consuming stage in determining the toxicological profile of new chemical entities. Within DART studies, morphological evaluation of fetal skeletons for developmental abnormalities typically requires 7 to 14 days. Current processing techniques involve digestion of soft tissue using a strong base (KOH), followed by qualitative assessment of the remaining skeletal tissue by a fetal morphologist...
August 3, 2017: Birth defects research
https://www.readbyqxmd.com/read/28762120/-the-zanzu-web-portal-a%C3%A2-contribution-to-the-sexual-health-of-migrants
#4
REVIEW
Ilona Renner, Christine Winkelmann
BACKGROUND: "Zanzu - my body in words and images" - zanzu.de is a multilingual web portal that brings together information about different aspects of sexual health. It was developed to address specific needs of migrants, including barriers to access. Zanzu provides quality-assured information on the topics of body, sexuality, HIV/STIs, family planning, sexuality and rights. METHOD: A few months after launching the web portal, a formative evaluation was conducted...
July 31, 2017: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
https://www.readbyqxmd.com/read/28760233/biochemical-and-imaging-diagnostics-in-endocrinology-predictors-of-fertility
#5
REVIEW
Erica B Mahany, John F Randolph
One of the limiting factors of fertility testing is the relative inefficiency of human reproduction. A careful history and physical examination must be performed on each patient to inform the particular diagnostic tests that are chosen and to create a meaningful treatment plan. Testing parameters, such as sensitivity, specificity, positive predictive value, and negative predictive value, can help to interpret test results, although there is no perfect screening test for the various causes of infertility. This article describes the 4 major categories of testing for infertility: ovarian reserve, ovulatory status, gamete transport, and male factor...
September 2017: Endocrinology and Metabolism Clinics of North America
https://www.readbyqxmd.com/read/28755995/the-diagnosis-and-surgical-approach-of-uterine-septae
#6
REVIEW
Aaron Budden, Jason Abbott
Uterine septae are a class of Müllerian duct anomaly that, similar to other classes, are associated with poor reproductive outcomes, however they are unique since these poor outcomes, particular recurrent pregnancy loss, may be responsive to surgical correction. The current evidence regarding septoplasty is difficult to interpret as the definitions of a uterine septum, methods to diagnose it, and hysteroscopic techniques used for treatment are variable and generally of poor to moderate quality. Robust data on outcomes, particularly live birth, after septoplasty are not available at this time...
July 26, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28754175/case-report-term-birth-after-fertility-sparing-treatments-for-stage-ib1-small-cell-neuroendocrine-carcinoma-of-the-cervix
#7
Pei-Ying Wu, Ya-Min Cheng, Geok Huey New, Cheng-Yang Chou, Chun-Ting Chiang, Hung-Wen Tsai, Yu-Fang Huang
BACKGROUND: Advances in cervical cancer management for childbearing women have led to less radical approaches. Use of fertility-sparing treatment to treat small cell neuroendocrine carcinoma (SCNEC) is challenging owing to the aggressive nature of the disease, even in early stage disease. CASE PRESENTATION: A 25-year-old nulligravida woman presented with malodorous vaginal discharge and was diagnosed to have an exophytic cervical SCNEC. A magnetic resonance image scan showed no evidence of parametrial invasion or distant metastasis...
July 28, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/28752522/pentoxifylline-increases-the-level-of-nitric-oxide-produced-by-human-spermatozoa
#8
S A Banihani, R F Abu-Alhayjaa, Z O Amarin, K H Alzoubi
Pentoxifylline (PF) is a xanthine derivative drug primarily used to treat peripheral vascular disorders. It is currently used in assisted reproductive technologies to enhance human sperm motility. However, the mechanism by which this enhancement occurs is not fully understood. Given that nitric oxide has been identified as a trigger to sperm motion, we asked whether nitric oxide modulates the stimulatory effect of PF on sperm motility. A total of 41 semen samples from infertile males were studied. Nitric oxide production in the presence of 5 mm PF was tested using different bio-analytical methods (spectrophotometry, fluorometry and fluorescence microscopy)...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28746933/androgen-regulates-dimorphic-f-actin-assemblies-in-the-genital-organogenesis
#9
Liqing Liu, Kentaro Suzuki, Eunice Chun, Aki Murashima, Yuki Sato, Naomi Nakagata, Toshihiko Fujimori, Shigenobu Yonemura, Wanzhong He, Gen Yamada
Impaired androgen activity induces defective sexual differentiation of the male reproductive tract, including hypospadias, an abnormal formation of the penile urethra. Androgen signaling in the urethral mesenchyme cells (UMCs) plays essential roles in driving dimorphic urethral development. However, cellular events for sexual differentiation remain virtually unknown. In this study, histological analyses, fluorescent staining, and transmission electron microscopy (TEM) were performed to reveal the cellular dimorphisms of UMCs...
July 27, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/28739122/idiopathic-granulomatous-mastitis-a-diagnostic-and-therapeutic-challenge
#10
C M Freeman, B T Xia, G C Wilson, J D Lewis, S Khan, S J Lee, E E Lower, M J Edwards, E A Shaughnessy
BACKGROUND: Idiopathic granulomatous mastitis is a rare benign breast disease of women of reproductive age. It usually presents as a painful mass. Since the etiology is unclear, directed diagnosis and management is lacking. METHODS: This is a retrospective chart review of 14 patients, over twelve years (2004-2016), identified through query of pathology findings. RESULTS: Two asymptomatic patients were diagnosed after oncologic breast resection following neoadjuvant chemotherapy...
July 8, 2017: American Journal of Surgery
https://www.readbyqxmd.com/read/28736946/fast-and-reproducible-in-vivo-t1-mapping-of-the-human-cervical-spinal-cord
#11
Marco Battiston, Torben Schneider, Ferran Prados, Francesco Grussu, Marios C Yiannakas, Sebastien Ourselin, Claudia A M Gandini Wheeler-Kingshott, Rebecca S Samson
PURPOSE: To develop a fast and robust method for measuring T1 in the whole cervical spinal cord in vivo, and to assess its reproducibility. METHODS: A spatially nonselective adiabatic inversion pulse is combined with zonally oblique-magnified multislice echo-planar imaging to produce a reduced field-of-view inversion-recovery echo-planar imaging protocol. Multi- inversion time data are obtained by cycling slice order throughout sequence repetitions. Measurement of T1 is performed using 12 inversion times for a total protocol duration of 7 min...
July 24, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28730506/hiatus-hernia-as-a-cause-of-dysphagia
#12
REVIEW
Hamish Philpott, Rami Sweis
PURPOSE OF REVIEW: This review aims to discuss the putative relationship between hiatus hernia and dysphagia. RECENT FINDINGS: Proposed mechanisms of dysphagia in patients with hiatus hernia are usually difficult to identify, but recent advances in technology (high-resolution manometry with or without concomitant impedance, ambulatory pH with impedance, videofluoroscopy, and the endoluminal functional lumen imaging probe (EndoFLIP)) and methodology (inclusion of swallows of various consistencies and volumes or shifting position during the manometry protocol) can help induce symptoms and identify the underlying disorder...
August 2017: Current Gastroenterology Reports
https://www.readbyqxmd.com/read/28729930/structural-differences-in-impaired-verbal-fluency-in-essential-tremor-patients-compared-to-healthy-controls
#13
Esther A Pelzer, Christian Nelles, David J Pedrosa, Carsten Eggers, Lothar Burghaus, Corina Melzer, Marc Tittgemeyer, Lars Timmermann
OBJECTIVE: We wanted to identify differences in grey and white matter in essential tremor patients compared to controls in the non-motor domain, using the example of impaired verbal fluency. BACKGROUND: A disturbance of verbal fluency in essential tremor patients compared to healthy controls is behaviorally well described. METHODS: Voxel-based morphometry and tract-based spatial statistics were used to analyze structural differences in grey and white matter in 19 essential tremor patients compared to 23 age- and gender-matched controls...
July 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28729622/three-dimensional-visualization-of-intrauterine-conceptus-through-the-uterine-wall-by-tissue-clearing-method
#14
Kyosuke Kagami, Yohei Shinmyo, Masanori Ono, Hiroshi Kawasaki, Hiroshi Fujiwara
Visualization of specific cells in the three-dimensional organ architecture is one of the key steps to develop our knowledge about pathophysiological mechanisms in various organs. In this study, we successfully obtained stereoscopic whole images of the intrauterine murine embryo and placenta through the uterus using a modified tissue clearing CUBIC method. By this procedure, we can recognize the three-dimensional relationships among various tissues within the pregnant uterus and analyze free-angle images of cross-sections with single-cell resolution using a computer system...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28727765/defining-the-target-and-the-effect-of-imatinib-on-the-filarial-c-abl-homologue
#15
Elise M O'Connell, Olena Kamenyeva, Sara Lustigman, Aaron Bell, Thomas B Nutman
BACKGROUND: Previously we demonstrated the micro- and macrofilaricidal properties of imatinib in vitro. Here we use electron and multiphoton microscopy to define the target of imatinib in the adult and microfilarial stages of Brugia malayi and assess the effects of pharmacologically relevant levels of imatinib on the adult parasites. METHODS: After fixation of adult B. malayi males and females, sections were stained with polyclonal rabbit anti-c-Abl antibody (or isotype control) and imaged with multiphoton fluorescent microscopy...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28722201/susceptibility-induced-distortion-correction-in-hyperpolarized-echo-planar-imaging
#16
Jack J Miller, Angus Z Lau, Damian J Tyler
PURPOSE: Echo planar imaging is an attractive rapid imaging readout that can image hyperpolarized compounds in vivo. By alternating the sign of the phase encoding gradient waveform, spatial offsets arising from uncertain frequency shifts can be determined. We show here that blip-reversed echo planar imaging can also be used to correct for susceptibility and B0 inhomogeneity effects that would otherwise produce image-domain distortion in the heart. METHODS: Previously acquired blip-reversed cardiac 3D-Spectral-Spatial echo planar imaging volumetric timecourses of hyperpolarized [1-(13) C]pyruvate were distortion corrected by a deformation field estimated by reconstructing signal-to-noise ratio (SNR)-weighted progressively subsampled temporally summed images of each metabolite...
July 19, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28711187/development-of-an-in-vitro-diaphragm-motion-reproduction-system
#17
Ai-Ho Liao, Ho-Chiao Chuang, Ming-Chih Shih, Hsiao-Yu Hsu, Der-Chi Tien, Chia-Chun Kuo, Shiu-Chen Jeng, Jeng-Fong Chiou
This study developed an in vitro diaphragm motion reproduction system (IVDMRS) based on noninvasive and real-time ultrasound imaging to track the internal displacement of the human diaphragm and diaphragm phantoms with a respiration simulation system (RSS). An ultrasound image tracking algorithm (UITA) was used to retrieve the displacement data of the tracking target and reproduce the diaphragm motion in real time using a red laser to irradiate the diaphragm phantom in vitro. This study also recorded the respiration patterns in 10 volunteers...
July 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28706850/pneumothorax-caused-by-cystic-and-nodular-lung-metastases-from-a-malignant-uterine-perivascular-epithelioid-cell-tumor-pecoma
#18
Shouichi Okamoto, Moegi Komura, Yasuhisa Terao, Aiko Kurisaki-Arakawa, Takuo Hayashi, Tsuyoshi Saito, Shinsaku Togo, Akira Shiokawa, Keiko Mitani, Etsuko Kobayashi, Toshio Kumasaka, Kazuhisa Takahashi, Kuniaki Seyama
Perivascular epithelioid cell tumors (PEComas) are mesenchymal neoplasms with immunoreactivity for both melanocytic and smooth muscle markers. PEComas occur at multiple sites, and malignant PEComas can undergo metastasis, recurrence and aggressive clinical courses. Although the lung is a common metastatic site of PEComas, they usually appear as multiple nodules but rarely become cystic or cavitary. Here, we describe a female patient whose lungs manifested multiple cystic, cavity-like and nodular metastases 3 years after the resection of uterine tumors tentatively diagnosed as epithelioid smooth muscle tumors with uncertain malignant potential...
2017: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/28705369/magnetic-resonance-imaging-of-pelvic-masses-a-compartmental-approach
#19
Kiran Gangadhar, Abhishek Mahajan, Nilesh Sable, Puneet Bhargava
Magnetic resonance imaging (MRI) is often "one stop shop" for evaluating female pelvic masses that helps in diagnosis, staging, and restaging of these tumors. A pelvic mass can arise from any tissue present within the pelvis. Although most masses in the female pelvis arise from the reproductive organs, masses may also arise from the gastrointestinal tract, urinary system, adjacent soft tissues, peritoneum, etc. It may not always be possible to determine the site of origin or distinguish these masses based on imaging characteristics...
June 2017: Seminars in Ultrasound, CT, and MR
https://www.readbyqxmd.com/read/28698386/antagonistic-serotonergic-and-octopaminergic-neural-circuits-mediate-food-dependent-locomotory-behavior-in-caenorhabditis-elegans
#20
Matthew A Churgin, Richard J McCloskey, Emily Peters, Christopher Fang-Yen
Biogenic amines are conserved signaling molecules that link food cues to behavior and metabolism in a wide variety of organisms. In the nematode C. elegans, the biogenic amines serotonin (5-HT) and octopamine regulate a number of food-related behaviors. Using a novel method for long-term quantitative behavioral imaging, we show that 5-HT and octopamine jointly influence locomotor activity and quiescence in feeding and fasting hermaphrodites, and we define the neural circuits through which this modulation occurs...
July 11, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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