keyword
MENU ▼
Read by QxMD icon Read
search

Reproductive Imaging

keyword
https://www.readbyqxmd.com/read/28938734/hyperspectral-microscopy-can-detect-metabolic-heterogeneity-within-bovine-post-compaction-embryos-incubated-under-two-oxygen-concentrations-7-versus-20
#1
Melanie L Sutton-McDowall, Martin Gosnell, Ayad G Anwer, Melissa White, Malcolm Purdey, Andrew D Abell, Ewa M Goldys, Jeremy G Thompson
STUDY QUESTION: Can we separate embryos cultured under either 7% or 20% oxygen atmospheres by measuring their metabolic heterogeneity? SUMMARY ANSWER: Metabolic heterogeneity and changes in metabolic profiles in morula exposed to two different oxygen concentrations were not detectable using traditional fluorophore and two-channel autofluorescence but were detectable using hyperspectral microscopy. WHAT IS KNOWN ALREADY: Increased genetic and morphological blastomere heterogeneity is associated with compromised developmental competence of embryos and currently forms the basis for embryo scoring within the clinic...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28936658/analysis-of-peroxisome-biogenesis-in-pollen-by-confocal-microscopy-and-transmission-electron-microscopy
#2
Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang
Peroxisome is an essential single-membrane bound organelle in most eukaryotic cells and functions in diverse cellular processes. De novo formation, division, and turnover of peroxisomes contribute to its biogenesis, morphology, and population regulation. In plants, peroxisome plays multiple roles, including metabolism, development, and stress response. Defective peroxisome biogenesis and development retard plant growth, adaption, and reproduction. Through tracing the subcellular localization of fluorescent reporter tagged matrix protein of peroxisome, fluorescence microscopy is a reliable and fast way to detect peroxisome biogenesis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936652/staining-and-clearing-of-arabidopsis-reproductive-tissue-for-imaging-of-fluorescent-proteins
#3
Daniel Slane, Patrick Bürgel, Martin Bayer
Imaging of fluorescent proteins in whole-mount tissue is a powerful tool to understand growth and developmental processes, not only in plants. With the advent of genetically encoded fluorescent reporters, which specifically label reproductive cells in Arabidopsis, deep tissue imaging has become increasingly important for the study of plant reproduction. To penetrate the surrounding layers of maternal tissue, however, the tissue has to be cleared by homogenizing the refractive index of the sample, often leading to inactivation of fluorescent proteins...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936648/live-cell-imaging-of-f-actin-dynamics-during-fertilization-in-arabidopsis-thaliana
#4
Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima
Fertilization comprises a complex series of cellular processes leading to the fusion of a male and female gamete. Many studies have been carried out to investigate each step of fertilization in plants; however, our comprehensive understanding of all the sequential events during fertilization is still limited. This is largely due to difficulty in investigating events in the female gametophyte, which is deeply embedded in the maternal tissue. Recent advances in confocal microcopy assisted by fluorescent marker lines have contributed to visualizing subcellular dynamics in real time during fertilization in vivo...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936647/3d-imaging-of-whole-mount-ovules-at-cellular-resolution-to-study-female-germline-development-in-rice
#5
Ethel Mendocilla-Sato, Wenjing She, Célia Baroux
Recent advances in fluorescence-based staining of cellular compartments coupled with confocal microscopy imaging have allowed the visualization of three-dimensional (3D) structures with cellular resolution in various intact plant tissues and species. Such approaches are of particular interest for the analysis of the reproductive lineage in plants including the meiotic precursor cells deeply embedded within the ovary of the gynoecium enclosed in the flower. Yet, their relative inaccessibility and the lack of optical clarity of plant tissues prevent robust staining and imaging across several cell layers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28934317/photosynthetic-epibionts-and-endobionts-of-pacific-oyster-shells-from-oyster-reefs-in-rocky-versus-mudflat-shores
#6
Laurent Barillé, Anthony Le Bris, Vona Méléder, Patrick Launeau, Marc Robin, Ioanna Louvrou, Lourenço Ribeiro
The Pacific oyster, Crassostrea gigas (Thunberg), is the main bivalve species cultivated in the world. With global warming enabling its reproduction and larval survival at higher latitudes, this species is now recognized as invasive and creates wild oyster reefs globally. In this study, the spatial distribution of photosynthetic assemblages colonizing the shells of wild C. gigas was investigated on both a large scale (two contrasting types of reefs found in mudflats and rocky areas) and a small scale (within individual shells) using a hyperspectral imager...
2017: PloS One
https://www.readbyqxmd.com/read/28921655/fast-dynamic-ventilation-mri-of-hyperpolarized-129-xe-using-spiral-imaging
#7
Ozkan Doganay, Tahreema N Matin, Anthony Mcintyre, Brian Burns, Rolf F Schulte, Fergus V Gleeson, Daniel Bulte
PURPOSE: To develop and optimize a rapid dynamic hyperpolarized (129) Xe ventilation (DXeV) MRI protocol and investigate the feasibility of capturing pulmonary signal-time curves in human lungs. THEORY AND METHODS: Spiral k-space trajectories were designed with the number of interleaves Nint  = 1, 2, 4, and 8 corresponding to voxel sizes of 8 mm, 5 mm, 4 mm, and 2.5 mm, respectively, for field of view = 15 cm. DXeV images were acquired from a gas-flow phantom to investigate the ability of Nint  = 1, 2, 4, and 8 to capture signal-time curves...
September 16, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28921614/an-optimized-framework-for-quantitative-magnetization-transfer-imaging-of-the-cervical-spinal-cord-in-vivo
#8
Marco Battiston, Francesco Grussu, Andrada Ianus, Torben Schneider, Ferran Prados, James Fairney, Sebastien Ourselin, Daniel C Alexander, Mara Cercignani, Claudia A M Gandini Wheeler-Kingshott, Rebecca S Samson
PURPOSE: To develop a framework to fully characterize quantitative magnetization transfer indices in the human cervical cord in vivo within a clinically feasible time. METHODS: A dedicated spinal cord imaging protocol for quantitative magnetization transfer was developed using a reduced field-of-view approach with echo planar imaging (EPI) readout. Sequence parameters were optimized based in the Cramer-Rao-lower bound. Quantitative model parameters (i.e., bound pool fraction, free and bound pool transverse relaxation times [ T2F, T2B], and forward exchange rate [kFB ]) were estimated implementing a numerical model capable of dealing with the novelties of the sequence adopted...
September 16, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28917264/expression-of-kisspeptin-protein-in-hypothalamus-and-lh-profile-of-growing-female-lambs
#9
Jolanta Polkowska, Anna Wójcik-Gładysz, Natalia Chmielewska, Marta Wańkowska
Kisspeptin (kp) is considered to be one of the major regulators of the induction of pubertal events via the activation of the gonadotrophin-releasing hormone-LH system. The aim of the present study was to analyse expression of immunoreactive (ir) kp in the hypothalamic neurons of female lambs from the neonatal to the peripubertal period (5 days to 32 weeks) in relation to the plasma LH pattern using immunohistochemistry and image analysis. Hypothalami were collected from female lambs (n=33) from the infantile, juvenile, prepubertal and peripubertal periods...
September 18, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28916873/effects-of-methotrexate-on-the-quality-of-oocyte-maturation-in-vitro
#10
N Tian, J Yu, Sh Zhang, W Y Ma, T Wang, Y M Wang
Methotrexate (MTX), an antifolate drug, is widely used for clinical treatment of malignancies and ectopic pregnancy. Many studies have documented that MTX has strong side-effects on rapidly dividing somatic cells. However, its side-effects on female reproductive cells have not been widely reported. Combined with in vitro culture, two-photon fluorescence imaging and three-dimensional reconstruction, this study analyzed the effects of MTX on oocyte maturation time, chromosome arrangement, karyotype, spindle morphology, and the localization of microtubule organizing centers (MTOCs)...
September 15, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28915543/effects-of-diisononyl-phthalate-on-danio-rerio-reproduction
#11
Stefania Santangeli, Francesca Maradonna, Maya Zanardini, Valentina Notarstefano, Giorgia Gioacchini, Isabel Forner-Piquer, Hamid Habibi, Oliana Carnevali
Di-isononyl phthalate (DiNP) is a high molecular weight phthalate commonly used as a plasticizer. It was introduced as a replacement for bis (2-ethylhexyl) phthalate (DEHP) which is used in the production of plasticized polyvinyl chloride (PVC). The purpose of this study was to investigate for the first time the effect of DiNP on female reproductive physiology in Danio rerio. Fish were exposed to five different doses of DiNP plus control (0 μg/L; 0.42 μg/L; 4.2 μg/L; 42 μg/L; 420 μg/L; 4200 μg/L) for a period of 21 days...
September 11, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28911214/growth-regulation-by-estrogen-in-breast-cancer-1-greb1-is-a-novel-progesterone-responsive-gene-required-for-human-endometrial-stromal-decidualization
#12
Alison J Camden, Maria M Szwarc, Sangappa B Chadchan, Francesco J DeMayo, Bert W O'Malley, John P Lydon, Ramakrishna Kommagani
STUDY QUESTION: Is Growth Regulation by Estrogen in Breast Cancer 1 (GREB1) required for progesterone-driven endometrial stromal cell decidualization? SUMMARY ANSWER: GREB1 is a novel progesterone-responsive gene required for progesterone-driven human endometrial stromal cell (HESC) decidualization. WHAT IS KNOWN ALREADY: Successful establishment of pregnancy requires HESCs to transform from fibroblastic to epithelioid cells in a process called decidualization...
September 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28899426/benign-metastasizing-leiomyoma-presenting-as-multiple-cystic-pulmonary-nodules-a-case-report
#13
Yeong Hun Choe, So Yeon Jeon, Yoon Chae Lee, Myung Ja Chung, Seung Yong Park, Yong Chul Lee, So Ri Kim
BACKGROUND: Benign metastatic leiomyoma (BML) is an extremely rare disease. Although uterine leiomyomas are benign histologically, they can metastasize to distant sites. While the incidence is very low, the lung is the organ most frequently affected by BML. Pulmonary BML usually presents as numerous well-defined nodules of various sizes, while the cavitary or cystic features in the nodules are rarely observed on radiologic images. CASE PRESENTATION: A 52-year-old woman complained of cough and dyspnea for one month...
September 12, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/28890972/nanomechanical-sensors-for-direct-and-rapid-characterization-of-sperm-motility-based-on-nanoscale-vibrations
#14
Shangquan Wu, Zhiguo Zhang, Xiarong Zhou, Hong Liu, Changguo Xue, Gang Zhao, Yunxia Cao, Qingchuan Zhang, Xiaoping Wu
Infertility, whether of male or female origin, is a critical challenge facing the low birth rate and aging population throughout the world, and semen analysis is a cornerstone of the diagnostic evaluation of the male contribution to infertility. This means that tools which can characterize sperm properties in an effective manner are very much needed. The conventional approaches are essentially image-based, which have a limited value for analyzing sperm properties. Here, we show that an assay using nanomechanical sensors can detect sperm motility based on nanomotion...
September 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28886590/corpora-lutea-in-superovulated-ewes-fed-different-planes-of-nutrition
#15
A Kraisoon, D A Redmer, C S Bass, C Navanukraw, S T Dorsam, V Valkov, A Reyaz, A T Grazul-Bilska
The corpus luteum (CL) is an ovarian structure which is critical for the maintenance of reproductive cyclicity and pregnancy support. Diet and/or diet components may affect some luteal functions. FSH is widely used to induce multiple follicle development and superovulation. We hypothesized that FSH would affect luteal function in ewes fed different nutritional planes. Therefore, the aim of this study was to determine if FSH-treatment affects (1) ovulation rate; (2) CL weight; (3) cell proliferation; (4) vascularity; (5) expression of endothelial nitric oxide (eNOS) and soluble guanylate cyclase (sGC) proteins; and (6) luteal and serum progesterone (P4) concentration in control (C), overfed (O), and underfed (U) ewes at the early- and mid-luteal phases...
August 12, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28880892/beyond-hiv-serodiscordance-partnership-communication-dynamics-that-affect-engagement-in-safer-conception-care
#16
Lynn T Matthews, Bridget F Burns, Francis Bajunirwe, Jerome Kabakyenga, Mwebesa Bwana, Courtney Ng, Jasmine Kastner, Annet Kembabazi, Naomi Sanyu, Adrine Kusasira, Jessica E Haberer, David R Bangsberg, Angela Kaida
INTRODUCTION: We explored acceptability and feasibility of safer conception methods among HIV-affected couples in Uganda. METHODS: We recruited HIV-positive men and women on antiretroviral therapy (ART) ('index') from the Uganda Antiretroviral Rural Treatment Outcomes cohort who reported an HIV-negative or unknown-serostatus partner ('partner'), HIV-serostatus disclosure to partner, and personal or partner desire for a child within two years. We conducted in-depth interviews with 40 individuals from 20 couples, using a narrative approach with tailored images to assess acceptability of five safer conception strategies: ART for the infected partner, pre-exposure prophylaxis (PrEP) for the uninfected partner, condomless sex timed to peak fertility, manual insemination, and male circumcision...
2017: PloS One
https://www.readbyqxmd.com/read/28877273/virtual-reality-simulator-for-dental-anesthesia-training-in-the-inferior-alveolar-nerve-block
#17
Cléber Gimenez Corrêa, Maria Aparecida de Andrade Moreira Machado, Edith Ranzini, Romero Tori, Fátima de Lourdes Santos Nunes
Objectives: This study shows the development and validation of a dental anesthesia-training simulator, specifically for the inferior alveolar nerve block (IANB). The system developed provides the tactile sensation of inserting a real needle in a human patient, using Virtual Reality (VR) techniques and a haptic device that can provide a perceived force feedback in the needle insertion task during the anesthesia procedure. Material and Methods: To simulate a realistic anesthesia procedure, a Carpule syringe was coupled to a haptic device...
July 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28871385/dynamic-3t-pelvic-floor-magnetic-resonance-imaging-in-women-progressing-from-the-nulligravid-to-the-primiparous-state
#18
Mark E Lockhart, G Wright Bates, Desiree E Morgan, Timothy M Beasley, Holly E Richter
INTRODUCTION AND HYPOTHESIS: The objective was to prospectively characterize dynamic pelvic 3-Tesla magnetic resonance imaging (dp3T MRI) findings in nulligravida women and characterize changes 6 months after delivery in the same woman. METHODS: In this prospective study, nulligravida women seeking assisted reproductive technology for pregnancy were recruited. After physical examination by Pelvic Organ Prolapse Quantification (POP-Q), Brink assessment and measures including the Pelvic Floor Distress Inventory-20 and Pelvic Floor Impact Questionnaire-7, pre-pregnancy dp3T MRI at rest, with strain, and evacuation were performed...
September 4, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28869478/tai-chi-chuan-and-baduanjin-increase-grey-matter-volume-in-older-adults-a-brain-imaging-study
#19
Jing Tao, Jiao Liu, Weilin Liu, Jia Huang, Xiehua Xue, Xiangli Chen, Jinsong Wu, Guohua Zheng, Bai Chen, Ming Li, Sharon Sun, Kristen Jorgenson, Courtney Lang, Kun Hu, Shanjia Chen, Lidian Chen, Jian Kong
The aim of this study is to investigate and compare how 12-weeks of Tai Chi Chuan and Baduanjin exercise can modulate brain structure and memory function in older adults. Magnetic resonance imaging and memory function measurements (Wechsler Memory Scale-Chinese revised, WMS-CR) were applied at both the beginning and end of the study. Results showed that both Tai Chi Chuan and Baduanjin could significantly increase grey matter volume (GMV) in the insula, medial temporal lobe, and putamen after 12-weeks of exercise...
August 30, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28861295/evaluation-of-the-diagnostic-efficacy-of-strain-elastography-in-infertile-population-with-normal-and-abnormal-semen-parameters
#20
Faruk Küçükdurmaz, Mehmet Akif Sarıca, Özcan Emre, Murat Baykara, Betül Kızıldağ, Sefa Resim
OBJECTIVE: The aim of the present study was to investigate the diagnostic value of strain elastography (SE) of testicular tissues in infertile population. We also evaluated the correlation between SE results with semen parameters and hormone profiles of the patients. MATERIAL AND METHODS: A total of 61 patients and 122 testes were evaluated. Patients who were evaluated in an andrology outpatient clinic with the diagnosis of infertility and referred to radiology department for investigation of reproductive organs between June 2015 and January 2016 were included...
September 2017: Turkish Journal of Urology
keyword
keyword
44496
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"