keyword
MENU ▼
Read by QxMD icon Read
search

Nudix

keyword
https://www.readbyqxmd.com/read/28167526/vibrio-phage-kvp40-encodes-a-functional-nad-salvage-pathway
#1
Jae Yun Lee, Zhiqun Li, Eric S Miller
: The genome of T4-type Vibrio bacteriophage KVP40 has five genes predicted to encode proteins of pyridine nucleotide metabolism, of which two, nadV and natV, would suffice for an NAD(+) salvage pathway. NadV is an apparent nicotinamide phosphoribosyltransferase (NAmPRTase) and NatV is an apparent bifunctional nicotinamide mononucleotide adenylyltransferase (NMNATase) and nicotinamide-adenine dinucleotide pyrophosphatase (Nudix hydrolase). Genes encoding the predicted salvage pathway were cloned, expressed in E...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28089905/nudix-type-motif-2-contributes-to-cancer-proliferation-through-the-regulation-of-rag-gtpase-mediated-mammalian-target-of-rapamycin-complex-1-localization
#2
Ohman Kwon, Dongoh Kwak, Sang Hoon Ha, Hyeona Jeon, Mangeun Park, Yeonho Chang, Pann-Ghill Suh, Sung Ho Ryu
Lysosomal localization of mammalian target of rapamycin complex 1 (mTORC1) is a critical step for activation of the molecule. Rag GTPases are essential for this translocation. Here, we demonstrate that Nudix-type motif 2 (NUDT2) is a novel positive regulator of mTORC1 activation. Activation of mTORC1 is impaired in NUDT2-silenced cells. Mechanistically, NUDT2 binds to Rag GTPase and controls mTORC1 translocation to the lysosomal membrane. Furthermore, NUDT2-dependent mTORC1 regulation is critical for proliferation of breast cancer cells, as NUDT2-silenced cells arrest in G0/G1 phases...
January 13, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28035004/structural-and-kinetic-studies-of-the-human-nudix-hydrolase-mth1-reveal-the-mechanism-for-its-broad-substrate-specificity
#3
Shaimaa Waz, Teruya Nakamura, Keisuke Hirata, Yukari Koga Ogawa, Mami Chirifu, Takao Arimori, Taro Tamada, Shinji Ikemizu, Yusaku Nakabeppu, Yuriko Yamagata
The human MutT homolog 1 (hMTH1, hNUDT1) hydrolyzes oxidatively damaged nucleoside triphosphates and is the main enzyme responsible for nucleotide sanitization. hMTH1 recently has received attention as an anti-cancer target because hMTH1 blockade leads to accumulation of oxidized nucleotides in the cell, resulting in mutations and death of cancer cells. Unlike Escherichia coli MutT, which shows high substrate specificity for 8-oxoguanine nucleotides, hMTH1 has broad substrate specificity for oxidized nucleotides, including 8-oxo-dGTP and 2-oxo-dATP...
December 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27974459/identification-of-the-rna-pyrophosphohydrolase-rpph-of-helicobacter-pylori-and-global-analysis-of-its-rna-targets
#4
Thorsten Bischler, Ping-Kun Hsieh, Marcus Resch, Quansheng Liu, Hock Siew Tan, Patricia L Foley, Anika Hartleib, Cynthia M Sharma, Joel G Belasco
RNA degradation is crucial for regulating gene expression in all organisms. Like the decapping of eukaryotic mRNAs, the conversion of the 5'-terminal triphosphate of bacterial transcripts to a monophosphate can trigger RNA decay by exposing the transcript to attack by 5'-monophosphate-dependent ribonucleases. In both biological realms, this deprotection step is catalyzed by members of the Nudix hydrolase family. The genome of the gastric pathogen Helicobacter pylori, a Gram-negative epsilonproteobacterium, encodes two proteins resembling Nudix enzymes...
February 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27936487/the-evolution-of-function-within-the-nudix-homology-clan
#5
John R Srouji, Anting Xu, Annsea Park, Jack F Kirsch, Steven E Brenner
The Nudix homology clan encompasses over 80,000 protein domains from all three domains of life, defined by homology to each other. Proteins with a domain from this clan fall into four general functional classes: pyrophosphohydrolases, isopentenyl diphosphate isomerases (IDIs), adenine/guanine mismatch-specific adenine glycosylases (A/G-specific adenine glycosylases), and non-enzymatic activities such as protein/protein interaction and transcriptional regulation. The largest group, pyrophosphohydrolases, encompasses more than 100 distinct hydrolase specificities...
December 9, 2016: Proteins
https://www.readbyqxmd.com/read/27874073/loss-of-function-of-an-arabidopsis-nadph-pyrophosphohydrolase-atnudx19-impacts-on-the-pyridine-nucleotides-status-and-confers-photooxidative-stress-tolerance
#6
Takanori Maruta, Takahisa Ogawa, Masaki Tsujimura, Keisuke Ikemoto, Tomofumi Yoshida, Hiro Takahashi, Kazuya Yoshimura, Shigeru Shigeoka
The levels and redox states of pyridine nucleotides, such as NADP(H), regulate the cellular redox homeostasis, which is crucial for photooxidative stress response in plants. However, how they are controlled is poorly understood. An Arabidopsis Nudix hydrolase, AtNUDX19, was previously identified to have NADPH hydrolytic activity in vitro, suggesting this enzyme to be a regulator of the NADPH status. We herein examined the physiological role of AtNUDX19 using its loss-of-function mutants. NADPH levels were increased in nudx19 mutants under both normal and high light conditions, while NADP(+) and NAD(+) levels were decreased...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27865623/key-role-of-an-adp-ribose-dependent-transcriptional-regulator-of-nad-metabolism-for-fitness-and-virulence-of-pseudomonas-aeruginosa
#7
Elza Okon, Sarah Dethlefsen, Anna Pelnikevich, Andrea van Barneveld, Antje Munder, Burkhard Tümmler
NAD is an essential co-factor of redox reactions and metabolic conversions of NAD-dependent enzymes. NAD biosynthesis in the opportunistic pathogen Pseudomonas aeruginosa has yet not been experimentally explored. The in silico search for orthologs in the P. aeruginosa PAO1 genome identified the operon pncA - pncB1-nadE (PA4918-PA4920) to encode the nicotinamidase, nicotinate phosporibosyltransferase and Nad synthase of salvage pathway I. The functional role of the preceding genes PA4917 and PA4916 was resolved by the characterization of recombinant protein...
November 5, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27778111/alr2954-of-anabaena-sp-pcc-7120-with-adp-ribose-pyrophosphatase-activity-bestows-abiotic-stress-tolerance-in-escherichia-coli
#8
Prashant Kumar Singh, Alok Kumar Shrivastava, Shilpi Singh, Ruchi Rai, Antra Chatterjee, L C Rai
In silico derived properties on experimental validation revealed that hypothetical protein Alr2954 of Anabaena sp. PCC7120 is ADP-ribose pyrophosphatase, which belongs to nudix hydrolase superfamily. Presence of ADP-ribose binding site was attested by ADP-ribose pyrophosphatase activity (K m 44.71 ± 8.043 mM, V max 7.128 ± 0.417 μmol min(-1) mg protein(-1), and K cat/K m 9.438 × 10(4) μM(-)1 min(-1)). Besides ADP-ribose, the enzyme efficiently hydrolyzed various nucleoside phosphatases such as 8-oxo-dGDP, 8-oxo-dADP, 8-oxo-dGTP, 8-oxo-dATP, GDP-mannose, ADP-glucose, and NADH...
January 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/27737001/correction-nudix-effectors-a-common-weapon-in-the-arsenal-of-plant-pathogens
#9
Suomeng Dong, Yuanchao Wang
[This corrects the article DOI: 10.1371/journal.ppat.1005704.].
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27683242/kinetic-and-mutational-studies-of-the-adenosine-diphosphate-ribose-hydrolase-from-mycobacterium-tuberculosis
#10
Suzanne F O'Handley, Puchong Thirawatananond, Lin-Woo Kang, Jennifer E Cunningham, J Alfonso Leyva, L Mario Amzel, Sandra B Gabelli
Mycobacterium tuberculosis represents one of the world's most devastating infectious agents - with one third of the world's population infected and 1.5 million people dying each year from this deadly pathogen. As part of an effort to identify targets for therapeutic intervention, we carried out the kinetic characterization of the product of gene rv1700 of M. tuberculosis. Based on its sequence and its structure, the protein had been tentatively identified as a pyrophosphohydrolase specific for adenosine diphosphate ribose (ADPR), a compound involved in various pathways including oxidative stress response and tellurite resistance...
December 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27618147/substrate-specificity-characterization-for-eight-putative-nudix-hydrolases-evaluation-of-criteria-for-substrate-identification-within-the-nudix-family
#11
Vi N Nguyen, Annsea Park, Anting Xu, John R Srouji, Steven E Brenner, Jack F Kirsch
The nearly 50,000 known Nudix proteins have a diverse array of functions, of which the most extensively studied is the catalyzed hydrolysis of aberrant nucleotide triphosphates. The functions of 171 Nudix proteins have been characterized to some degree, although physiological relevance of the assayed activities has not always been conclusively demonstrated. We investigated substrate specificity for eight structurally characterized Nudix proteins, whose functions were unknown. These proteins were screened for hydrolase activity against a 74-compound library of known Nudix enzyme substrates...
December 2016: Proteins
https://www.readbyqxmd.com/read/27550217/genome-analysis-and-avirulence-gene-cloning-using-a-high-density-radseq-linkage-map-of-the-flax-rust-fungus-melampsora-lini
#12
Claire Anderson, Muhammad Adil Khan, Ann-Maree Catanzariti, Cameron A Jack, Adnane Nemri, Gregory J Lawrence, Narayana M Upadhyaya, Adrienne R Hardham, Jeffrey G Ellis, Peter N Dodds, David A Jones
BACKGROUND: Rust fungi are an important group of plant pathogens that cause devastating losses in agricultural, silvicultural and natural ecosystems. Plants can be protected from rust disease by resistance genes encoding receptors that trigger a highly effective defence response upon recognition of specific pathogen avirulence proteins. Identifying avirulence genes is crucial for understanding how virulence evolves in the field. RESULTS: To facilitate avirulence gene cloning in the flax rust fungus, Melampsora lini, we constructed a high-density genetic linkage map using single nucleotide polymorphisms detected in restriction site-associated DNA sequencing (RADseq) data...
2016: BMC Genomics
https://www.readbyqxmd.com/read/27513453/nudix-effectors-a-common-weapon-in-the-arsenal-of-plant-pathogens
#13
Suomeng Dong, Yuanchao Wang
No abstract text is available yet for this article.
August 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27467751/a-novel-plant-enzyme-with-dual-activity-an-atypical-nudix-hydrolase-and-a-dipeptidyl-peptidase-iii
#14
Zrinka Karačić, Bojana Vukelić, Gabrielle H Ho, Iva Jozić, Iva Sučec, Branka Salopek-Sondi, Marija Kozlović, Steven E Brenner, Jutta Ludwig-Müller, Marija Abramić
In a search for plant homologues of dipeptidyl peptidase III (DPP III) family, we found a predicted protein from the moss Physcomitrella patens (UniProt entry: A9TLP4), which shared 61% sequence identity with the Arabidopsis thaliana uncharacterized protein, designated Nudix hydrolase 3. Both proteins contained all conserved regions of the DPP III family, but instead of the characteristic hexapeptide HEXXGH zinc-binding motif, they possessed a pentapeptide HEXXH, and at the N-terminus, a Nudix box, a hallmark of Nudix hydrolases, known to act upon a variety of nucleoside diphosphate derivatives...
January 1, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/27428510/structure-and-function-of-the-bacterial-decapping-enzyme-nudc
#15
Katharina Höfer, Sisi Li, Florian Abele, Jens Frindert, Jasmin Schlotthauer, Julia Grawenhoff, Jiamu Du, Dinshaw J Patel, Andres Jäschke
RNA capping and decapping are thought to be distinctive features of eukaryotes. The redox cofactor NAD was recently discovered to be attached to small regulatory RNAs in bacteria in a cap-like manner, and Nudix hydrolase NudC was found to act as a NAD-decapping enzyme in vitro and in vivo. Here, crystal structures of Escherichia coli NudC in complex with substrate NAD and with cleavage product NMN reveal the catalytic residues lining the binding pocket and principles underlying molecular recognition of substrate and product...
September 2016: Nature Chemical Biology
https://www.readbyqxmd.com/read/27425147/new-insights-into-decapping-enzymes-and-selective-mrna-decay
#16
REVIEW
Ewa Grudzien-Nogalska, Megerditch Kiledjian
Removal of the 5' end cap is a critical determinant controlling mRNA stability and efficient gene expression. Removal of the cap is exquisitely controlled by multiple direct and indirect regulators that influence association with the cap and the catalytic step. A subset of these factors directly stimulate activity of the decapping enzyme, while others influence remodeling of factors bound to mRNA and indirectly stimulate decapping. Furthermore, the components of the general decapping machinery can also be recruited by mRNA-specific regulatory proteins to activate decapping...
January 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/27406238/enpp1-processes-protein-adp-ribosylation-in-vitro
#17
Luca Palazzo, Casey M Daniels, Joanne E Nettleship, Nahid Rahman, Robert Lyle McPherson, Shao-En Ong, Kazuki Kato, Osamu Nureki, Anthony K L Leung, Ivan Ahel
ADP-ribosylation is a conserved post-translational protein modification that plays a role in all major cellular processes, particularly DNA repair, transcription, translation, stress response and cell death. Hence, dysregulation of ADP-ribosylation is linked to the physiopathology of several human diseases including cancers, diabetes and neurodegenerative disorders. Protein ADP-ribosylation can be reversed by the macrodomain-containing proteins PARG, TARG1, MacroD1 and MacroD2, which hydrolyse the ester bond known to link proteins to ADP-ribose as well as consecutive ADP-ribose subunits; targeting this bond can thus result in the complete removal of the protein modification or the conversion of poly(ADP-ribose) to mono(ADP-ribose)...
September 2016: FEBS Journal
https://www.readbyqxmd.com/read/27383051/the-proposed-channel-enzyme-transient-receptor-potential-melastatin-2-does-not-possess-adp-ribose-hydrolase-activity
#18
Iordan Iordanov, Csaba Mihályi, Balázs Tóth, László Csanády
Transient Receptor Potential Melastatin 2 (TRPM2) is a Ca(2+)-permeable cation channel essential for immunocyte activation, insulin secretion, and postischemic cell death. TRPM2 is activated by ADP ribose (ADPR) binding to its C-terminal cytosolic NUDT9-homology (NUDT9H) domain, homologous to the soluble mitochondrial ADPR pyrophosphatase (ADPRase) NUDT9. Reported ADPR hydrolysis classified TRPM2 as a channel-enzyme, but insolubility of isolated NUDT9H hampered further investigations. Here we developed a soluble NUDT9H model using chimeric proteins built from complementary polypeptide fragments of NUDT9H and NUDT9...
2016: ELife
https://www.readbyqxmd.com/read/27333281/adp-ribose-activates-the-trpm2-channel-from-the-sea-anemone-nematostella-vectensis-independently-of-the-nudt9h-domain
#19
Frank J P Kühn, Cornelia Kühn, Mathis Winking, Daniel C Hoffmann, Andreas Lückhoff
The human redox-sensitive Transient receptor potential melastatin type 2 (hTRPM2) channel contains the C-terminal Nudix hydrolase domain NUDT9H which most likely binds ADP-ribose. During oxidative stress, the intracellular release of ADP-ribose triggers the activation of hTRPM2. The TRPM2 orthologue from Nematostella vectensis (nv) is also stimulated by ADP-ribose but not by the oxidant hydrogen peroxide. For further clarification of the structure-function relationships of these two distantly related channel orthologues, we performed whole-cell as well as single channel patch-clamp recordings, Ca2+-imaging and Western blot analysis after heterologous expression of wild-type and mutated channels in HEK-293 cells...
2016: PloS One
https://www.readbyqxmd.com/read/27095738/modulation-of-nadh-levels-by-arabidopsis-nudix-hydrolases-atnudx6-and-7-and-the-respective-proteins-themselves-play-distinct-roles-in-the-regulation-of-various-cellular-responses-involved-in-biotic-abiotic-stresses
#20
Takahisa Ogawa, Kohei Muramoto, Risa Takada, Shouya Nakagawa, Shigeru Shigeoka, Kazuya Yoshimura
Arabidopsis Nudix hydrolases, AtNUDX6 and 7, exhibit pyrophosphohydrolase activities toward NADH and contribute to the modulation of various defense responses, such as the poly(ADP-ribosyl)ation (PAR) reaction and salicylic acid (SA)-induced Nonexpresser of Pathogenesis-Related genes 1 (NPR1)-dependent defense pathway, against biotic and abiotic stresses. However, the mechanisms by which these enzymes regulate such cellular responses remain unclear. To clarify the functional role(s) of AtNUDX6 and 7 and NADH metabolism, we examined the effects of the transient expression of the active and inactive forms of AtNUDX6 and 7 under the control of an estrogen (ES)-inducible system on various stress responses...
June 2016: Plant & Cell Physiology
keyword
keyword
44468
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"