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Ochratoxin

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https://www.readbyqxmd.com/read/28735045/multiplexed-capillary-microfluidic-immunoassay-with-smartphone-data-acquisition-for-parallel-mycotoxin-detection
#1
Jessica M D Machado, Ruben R G Soares, Virginia Chu, João P Conde
The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure...
July 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28732344/visible-light-driven-label-free-photoelectrochemical-immunosensor-based-on-tio2-s-bivo4-ag2s-nanocomposites-for-sensitive-detection-ota
#2
Jinhui Feng, Yueyun Li, Zengqiang Gao, Hui Lv, Xiaobo Zhang, Dawei Fan, Qin Wei
A label-free photoelectrochemical (PEC) platform with high visible-light activity for quantitative detection of the ochratoxin A (OTA) was developed by assembly of Ag2S nanoparticles (NPs) sensitized on titanium dioxide/red blood cell-like shape bismuth vanadate (TiO2/S-BiVO4) electrode via layer-by-layer (LBL) strategy. In this protocol, ascorbic acid was used as an efficient electron donor for scavenging photo-generated holes and inhibiting light driven electron-hole pair recombination. TiO2 has good photoelectric activity and large surface area...
July 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28719194/molecular-modeling-of-the-major-dna-adduct-formed-from-the-food-mutagen-ochratoxin-a-in-nari-2-base-deletion-duplexes-impact-of-sequence-context-and-adduct-ionization-on-conformational-preference-and-mutagenicity
#3
Preetleen Kathuria, Purshotam Sharma, Richard A Manderville, Stacey D Wetmore
Exposure to ochratoxin A (OTA), a possible human carcinogen, leads to many different DNA mutations. As a first step toward understanding the structural basis of OTA-induced mutagenicity, the present work uses a robust computational approach and a slipped mutagenic intermediate model previously studied for C(8)-dG aromatic amine adducts to analyze the conformational features of post-replication 2-base deletion DNA duplexes containing OT-dG, the major OTA lesion at the C(8) position of guanine. Specifically, a total of 960 ns of molecular dynamics simulations (excluding trial simulations) were carried out on four OT-dG ionization states in three sequence contexts within oligomers containing the NarI recognition sequence, a known hotspot for deletion mutations induced by related adducts formed from known carcinogens...
July 18, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28712913/acute-ochratoxin-a-exposure-induces-inflammation-and-apoptosis-in-human-embryonic-kidney-hek293-cells
#4
Shanel Raghubeer, Savania Nagiah, Anil A Chuturgoon
Ochratoxin A (OTA), a common contaminant of grain and fruit and known carcinogen, has been linked to impaired antioxidant response and cellular repair. The effect of OTA on inflammation in cells has not been explored. This study investigated OTA's influence on inflammatory mediators using a range of OTA concentrations (0.5 μM (sub-IC50), 1.2 μm (IC50) and 2 μm (supra-IC50)) on human embryonic kidney (HEK293) cells over 24hr. The markers of inflammation in HEK293 cells were evaluated using the following techniques: western blotting (phosphorylated (p-)NFκB (Ser536), p-IKK (Ser176/180) and p-p53 (Ser392), total NFκB, IKK, IκBα and p53), luminometry (caspases 1, 3/7, 8, 9, ATP) and ELISA to determine IL-1β levels...
July 14, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28710020/nrf2-deficiency-exacerbates-ochratoxin-a-induced-toxicity-in-vitro-and-in-vivo
#5
Agnieszka Loboda, Anna Stachurska, Mateusz Sobczak, Paulina Podkalicka, Olga Mucha, Alicja Jozkowicz, Jozef Dulak
Several mechanisms are postulated to be responsible for nephrotoxic and nephrocarcinogenic activities of mycotoxin and food contaminant, ochratoxin A (OTA). Although Nrf2 transcription factor was suggested to be involved in OTA-mediated renal injury, comprehensive study evaluating the effect of OTA toxicity in Nrf2 knock-out mice with special regard to sex-dependency has not been performed yet. Our results clearly show exacerbated OTA toxicity in porcine tubular epithelial cells after shRNA-mediated Nrf2 inhibition as well as in proximal tubular cells isolated from Nrf2(-/-) male mice in comparison to cells derived from their wild-type counterparts...
July 12, 2017: Toxicology
https://www.readbyqxmd.com/read/28708280/biodegradation-of-ochratoxin-a-by-alcaligenes-faecalis-isolated-from-soil
#6
H H Zhang, Y Wang, C Zhao, J Wang, X L Zhang
AIMS: The aim of the present work is to report on the hydrolytic action of Alcaligenes faecalis isolated from soil samples and its ability to degrade ochratoxin A. METHODS AND RESULTS: An Alcaligenes faecalis strain was identified and characterized by employing both a phenotypic analysis and 16S rDNA sequence analysis. The results show that this strain could degrade ochratoxin A efficiently but could not use it as a sole carbon source. Ochratoxin α was confirmed as a degradation product in the intracellular extract of Alcaligenes faecalis using UPLC-MS/MS...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28708102/ability-of-soil-isolated-actinobacterial-strains-to-prevent-bind-and-biodegrade-ochratoxin-a
#7
Rachelle El Khoury, Florence Mathieu, Ali Atoui, Hiba Kawtharani, Anthony El Khoury, Charbel Afif, Richard G Maroun, André El Khoury
Ochratoxin A (OTA) is one of the most important mycotoxins, and contaminates several agricultural products, particularly cereals, grapes, maize, barley, spices and coffee. The aim of this project was to reduce the levels of OTA by supplementing the artificially contaminated solutions with seven strains of actinobacteria (AT10, AT8, SN7, MS1, ML5, G10 and PT1) in order to evaluate their capacity for binding and metabolizing the OTA, as well as their ability to reduce the expression of the genes responsible for its production in A...
July 14, 2017: Toxins
https://www.readbyqxmd.com/read/28708032/comparison-of-a-newly-developed-liquid-chromatography-with-tandem-mass-spectrometry-method-and-enzyme-linked-immunosorbent-assay-for-detection-of-multiple-mycotoxins-in-red-pepper-powder
#8
Sunyoung Kim, Sanghee Lee, Tae-Gyu Nam, Dongwon Seo, Miyoung Yoo
In this study, we aimed to establish a method for determination of multiple mycotoxins such as aflatoxins, ochratoxin A, and zearalenone in red pepper powder samples based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for extraction and cleanup, with detection and quantification by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in both positive- and negative-ion modes. The developed LC-MS/MS analytical method was compared with an enzyme-linked immunosorbent assay (ELISA) to improve the reliability of our developed method...
July 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28699257/effects-of-ochratoxin-a-on-er-stress-mapk-signaling-pathway-and-autophagy-of-kidney-and-spleen-in-pigs
#9
Fang Gan, Lili Hou, Yajiao Zhou, Yunhuan Liu, Da Huang, Xingxiang Chen, Kehe Huang
Ochratoxin A (OTA), a worldwide mycotoxin found in food and feeds, is a potent nephrotoxin and immunotoxin in animals and humans. This research was conducted to evaluate whether endoplasmic reticulum (ER) stress, MAPK signaling pathway and autophagy were induced by OTA in kidney and spleen of pigs. Twenty-seven crossbred pigs randomly allocated to 3 groups were fed for 42 days ad libitum a basal diet without (Con group, 0.00 μg OTA/kg) and with supplementation of OTA at 400 (OTA-L group) and 800 μg/kg (OTA-H group)...
July 12, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28698507/risk-assessment-of-mycotoxins-in-stored-maize-grains-consumed-by-infants-and-young-children-in-nigeria
#10
Modupeade C Adetunji, Olusegun O Atanda, Chibundu N Ezekiel
Maize is a major complimentary food for infants (0-4years) and young children (5-12years) in Nigeria. In this study, we assessed the risk of exposure of infants and young children (IYC) to some major mycotoxins in stored maize grains from five agro-ecological zones of Nigeria. The probable daily intake approach was employed to determine exposure to five mycotoxins while the margin of exposure (MOE) and population at risk of primary hepatocellular carcinoma approaches were used to characterize the risk of consuming aflatoxin contaminated maize...
July 10, 2017: Children
https://www.readbyqxmd.com/read/28698493/antifungal-and-antiochratoxigenic-activities-of-essential-oils-and-total-phenolic-extracts-a-comparative-study
#11
Rachelle El Khoury, Ali Atoui, Florence Mathieu, Hiba Kawtharani, Anthony El Khoury, Richard G Maroun, Andre El Khoury
This study is intended to prevent ochratoxin A (OTA) production by Aspergillus carbonarius S402 using essential oils (EOs) and total phenolic compounds extracted from plants and herbs. The EOs used in this study are the following: bay leaves, cumin, fenugreek, melissa, mint, and sage. As for the phenolic compounds, they were extracted from bay leaves, cumin, fenugreek, melissa, mint, sage, anise, chamomile, fennel, rosemary, and thyme. The experiments were conducted on Synthetic Grape Medium (SGM) medium at 28 °C for 4 days...
July 9, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28697933/a-simple-method-for-point-of-need-extraction-concentration-and-rapid-multi-mycotoxin-immunodetection-in-feeds-using-aqueous-two-phase-systems
#12
Ruben R G Soares, Ana M Azevedo, Pedro Fernandes, Virginia Chu, João P Conde, M Raquel Aires-Barros
The rapid detection of mycotoxins in feed samples is becoming an increasingly relevant challenge for the food production sector, in order to effectively enforce current regulations and assure food and feed safety. To achieve rapid mycotoxin detection, several biosensing strategies have been published, many reaching assay times of the order of a few minutes. However, the vast majority of these rely on sample preparation based on volatile organic solvents, often comprising complex multi-step procedures and devoid of clean-up and/or concentration effects...
July 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28688805/low-level-of-ochratoxin-a-affects-genome-wide-expression-in-kidney-of-pig
#13
Daniela Eliza Marin, Cornelia Braicu, Mihai Alexandru Gras, Gina Cecilia Pistol, Roxana Cojocneanu Petric, Ioana Berindan Neagoe, Mihai Palade, Ionelia Taranu
Ochratoxin A (OTA) is a mycotoxin produced by fungus belonging to Aspergillus and Penicillium genra. The aim of the present paper was to investigate if a low concentration OTA has toxic effect in pigs. Twelve piglets were fed with a control or an OTA (0.05 mg/kg feed) contaminated diet. After 30 days, animals were slaughtered and samples of blood and kidney were used for further analyses. The mycotoxin analyses showed a significant higher (6.25 times) concentration of OTA in the kidney of OTA intoxicated piglets than in control ones...
July 6, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28672847/survey-of-alternaria-toxins-and-other-mycotoxins-in-dried-fruits-in-china
#14
Dizhe Wei, Yao Wang, Dongmei Jiang, Xiaoyuan Feng, Jun Li, Meng Wang
Occurrence of toxigenic molds and mycotoxins on dried fruits is a worldwide problem, but limited information is available in China. A total of 220 dried fruits (raisins, dried apricots, dates and wolfberries) purchased from China were analyzed for 17 mycotoxins (i.e., Alternaria toxins, ochratoxin A (OTA), patulin (PAT) and trichothecenes) by UPLC-MS/MS, combined with a single-step cleanup. The result showed that at least one mycotoxin was detected in 142 samples (64.6%). The lowest incidence of contaminated samples was observed in dried apricots (48...
June 26, 2017: Toxins
https://www.readbyqxmd.com/read/28672192/ultrasensitive-detection-of-ochratoxin-a-using-aptasensors
#15
REVIEW
Hasan Badie Bostan, Noor Mohammad Danesh, Gholamreza Karimi, Mohammad Ramezani, Seyed Ali Mousavi Shaegh, Kazem Youssefi, Fahimeh Charbgoo, Khalil Abnous, Seyed Mohammad Taghdisi
Regarding teratogenic, carcinogenic, and immunotoxic nature of ochratoxin A (OTA), selective and sensitive monitoring of this molecule in food samples is of great importance. In recent years, various methods have been introduced for detection of OTA. However, they are usually time-consuming, labor-intensive and expensive. Therefore, these parameters limited their usage. The emerging method of detection, aptasensor, has attracted more attention for OTA detection, due to distinctive advantages including high sensitivity, selectivity and simplicity...
June 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28665799/lycopene-restores-trace-element-levels-in-ochratoxin-a-treated-rats
#16
Saziye Sezin Palabiyik, Pinar Erkekoglu, Murat Kızılgun, Gonul Sahin, Belma Kocer-Gumusel
This study was designed to investigate the in vivo effects of ochratoxin A (OTA) and/or lycopene on the levels of selenium, zinc, and copper in the liver, kidneys, and testes of male Sprague-Dawley rats. The rats were treated with OTA (0.5 mg kg-1 day-1) and/or lycopene (5 mg kg-1 day-1) by gavage for 7 or 14 days. Trace element levels were measured by atomic absorption spectrometry. OTA significantly lowered selenium (20 % in the liver, 17 % in the kidney, and 40 % in the testis), zinc (24 % in the liver, 23 % in the kidney, and 26 % in the testis), and copper levels (40 % in the liver and 10 % in the kidney)...
June 27, 2017: Arhiv za Higijenu Rada i Toksikologiju
https://www.readbyqxmd.com/read/28661479/ochratoxin-a-induced-autophagy-in-vitro-and-in-vivo-promotes-porcine-circovirus-type-2-replication
#17
Gang Qian, Dandan Liu, Junfa Hu, Fang Gan, Lili Hou, Xingxiang Chen, Kehe Huang
Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium. Porcine circovirus type 2 (PCV2) is recognized as the causative agent of porcine circovirus-associated diseases. Recently, we reported that low doses of OTA promoted PCV2 replication in vitro and in vivo, but the underlying mechanism needed further investigation. The present studies further confirmed OTA-induced PCV2 replication promotion as measured by cap protein expression, viral titer, viral DNA copies and the number of infected cells...
June 29, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28661468/study-on-the-association-among-mycotoxins-and-other-variables-in-children-with-autism
#18
Barbara De Santis, Maria Elisabetta Raggi, Giorgio Moretti, Francesco Facchiano, Alessandra Mezzelani, Laura Villa, Arianna Bonfanti, Alessandra Campioni, Stefania Rossi, Serena Camposeo, Sabina Soricelli, Gabriele Moracci, Francesca Debegnach, Emanuela Gregori, Francesca Ciceri, Luciano Milanesi, Anna Marabotti, Carlo Brera
Environmental factors and genetic susceptibility are implicated in the increased risk of autism spectrum disorder (ASD). Mycotoxins are agricultural contaminants of fungal origin that represent real risk factors for human health and especially for children. Thus, the main hypothesis of this work is that the deterioration of the clinical manifestation of autism in children may result from the exposure to mycotoxins through the consumption of contaminated food. Within a cross-sectional study, a group of autistic children (n = 172) and a group of controls (n = 61) (siblings and non-parental) were recruited in North and South Italy...
June 29, 2017: Toxins
https://www.readbyqxmd.com/read/28650663/overexpression-and-lowexpression-of-selenoprotein-s-impact-ochratoxin-a-induced-porcine-cytotoxicity-and-apoptosis-in-vitro
#19
Fang Gan, Zhihua Hu, Yajiao Zhou, Kehe Huang
Our previous study demonstrated that selenium could alleviate ochratoxin A (OTA)-induced nephrotoxicity in PK15 cells. Selenoprotein S (SelS) has antioxidant activities, but it is unclear whether SelS plays a role in the alleviating effects of selenium on OTA-induced nephrotoxicity. We previously have stably transfected pig pCDNA3.1-SelS to PK15 cells to over-express SelS. Here, we demonstrated that SelS overexpression alleviated OTA-induced cytotoxicity and apoptosis as demonstrated by cell viabilities, LDH activities, Annexin V-bing, caspase 3 activities and apoptotic nuclei...
June 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28650611/integrating-target-responsive-hydrogel-with-pressuremeter-readout-enables-simple-sensitive-user-friendly-quantitative-point-of-care-testing
#20
Dan Liu, Shasha Jia, Huimin Zhang, Yanli Ma, Zhichao Guan, Jiuxing Li, Zhi Zhu, Tianhai Ji, Chaoyong James Yang
Point-of-care testing (POCT) with the advantages of speed, simplicity, and low cost, as well as no need for instrumentation, is critical for the measurement of analytes in a variety of environments lacking access to laboratory infrastructure. In the present study, a hydrogel pressure-based assay for quantitative POCT was developed by integrating a target-responsive hydrogel with pressuremeter readout. The target-responsive hydrogels were constructed with DNA grafted linear polyacrylamide and the cross-linking DNA for selective target recognition...
July 12, 2017: ACS Applied Materials & Interfaces
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