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Ochratoxin

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https://www.readbyqxmd.com/read/28107910/nanobody-based-enzyme-immunoassay-for-ochratoxin-a-in-cereal-with-high-resistance-to-matrix-interference
#1
Xing Liu, Zongwen Tang, Zhenhua Duan, Zhenyun He, Mei Shu, Xianxian Wang, Shirley J Gee, Bruce D Hammock, Yang Xu
A sensitive indirect competitive nanobody-based enzyme linked immunosorbent assay (Nb-ELISA) for ochratoxin A (OTA) with high resistance to cereal matrix interference was developed. Nanobodies against OTA (Nb15, Nb28, Nb32, Nb36) were expressed in E. coli cells and their thermal stabilities were compared with that of an OTA-specific monoclonal antibody 6H8. All nanobodies could still retain their antigen-binding activity after exposure to temperature 95°C for 5min or to 90°C for 75min. Nb28 that exhibited the highest sensitivity in ELISA was selected for further research...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28094577/protective-role-of-bentonite-against-aflatoxin-b1-and-ochratoxin-a-induced-immunotoxicity-in-broilers
#2
Sheraz Ahmed Bhatti, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Muhammad Saqib, Ahrar Khan, Zahoor Ul-Hassan
The present study was designed to investigate any ameliorative effects of bentonite (BN) against immuno-pathological alterations induced by dietary aflatoxin B1 (AFB1) or ochratoxin A (OTA) in broiler chicks. In one experiment, AFB1 (0.1, 0.2 or 0.6 mg/kg feed) was fed alone and par alley with bentonite clay (3.7 or 7.5 g/kg feed) to the broilers. In the second experiment, the broilers were given feed contaminated with OTA (0.15, 0.3 or 1.0 mg/kg feed) alone and in combination with bentonite clay (3...
January 17, 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28089712/effect-of-heme-oxygenase-1-on-ochratoxin-a-induced-nephrotoxicity-in-mice
#3
Agnieszka Loboda, Anna Stachurska, Paulina Podkalicka, Mateusz Sobczak, Olga Mucha, Agnieszka Witalisz-Siepracka, Alicja Jozkowicz, Jozef Dulak
Heme oxygenase-1 (HO-1), a heme-degrading enzyme, is suggested to play an important role in kidney pathophysiology, mostly due to its anti-fibrotic, anti-apoptotic and anti-oxidant properties. One of the mycotoxin, ochratoxin A (OTA) was previously shown to affect HO-1 expression, however, the mechanisms of OTA-induced nephrotoxicity during HO-1 deficiency are unknown. We have shown that OTA regulates the number of pro-fibrotic, pro-inflammatory, anti-oxidative and pro-apoptotic factors in HO-1 dependent manner, as the lack of HO-1 accelerates whereas the induction of HO-1 expression by cobalt protoporphyrin (CoPP) attenuates nephrotoxic effect of OTA...
January 9, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28053655/an-investigation-of-fungal-contamination-on-the-surface-of-medicinal-herbs-in-china
#4
Run-Sheng Zheng, Wen-Li Wang, Jing Tan, Hui Xu, Ruo-Ting Zhan, Wei-Wen Chen
BACKGROUND: The dried parts of medicinal herbs are susceptible to the infection of fungi during pre- or post-harvest procedure. This study aimed to investigate the presence of fungi and their metabolites mycotoxins on the surface of medicinal herbs collected from China. METHODS: Forty-five retail samples of 15 different medicinal herbs were collected from 3 different regions in China. Then the potential fungi were immediately washed off from the surface of each sample with 0...
2017: Chinese Medicine
https://www.readbyqxmd.com/read/28042000/mycotoxigenic-potentials-of-the-genera-aspergillus-fusarium-and-penicillium-isolated-from-houseflies-musca-domestica-l
#5
J Z Phoku, T G Barnard, N Potgieter, M F Dutton
A study on the potential of houseflies (Musca domestica L.) to spread fungal spores in Gauteng Province, South Africa proved that houseflies are vectors for fungal spores. Therefore, there is a need to determine the toxigenic potentials and to identify the mycotoxins produced by fungal isolates derived from this study. In total 377 potentially toxigenic isolates of Aspergillus (186), Fusarium (85) and Penicillium (106) species (spp.) were isolated. These isolates were further tested for their ability to produce aflatoxins (AFs) [aflatoxin B1, B2, G1 and G2], deoxynivalenol (DON), fumonisin B1 (FB1) ochratoxin A (OTA), and zearalenone (ZEA) by high-performance liquid chromatography (HPLC) respectively...
December 29, 2016: Acta Tropica
https://www.readbyqxmd.com/read/28041933/a-risk-assessment-of-dietary-ochratoxin-a-in-the-united-states
#6
Nicole J Mitchell, Chen Chen, Jeffrey D Palumbo, Andreia Bianchini, Jack Cappozzo, Jayne Stratton, Dojin Ryu, Felicia Wu
Ochratoxin A (OTA) is a mycotoxin (fungal toxin) found in multiple foodstuffs. Because OTA has been shown to cause kidney disease in multiple animal models, several governmental bodies around the world have set maximum allowable levels of OTA in different foods and beverages. In this study, we conducted the first exposure and risk assessment study of OTA for the United States' population. A variety of commodities from grocery stores across the US were sampled for OTA over a 2-year period. OTA exposure was calculated from the OTA concentrations in foodstuffs and consumption data for different age ranges...
December 30, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28038445/ochratoxin-a-induces-er-stress-and-apoptosis-in-mesangial-cells-via-a-nadph-oxidase-derived-reactive-oxygen-species-mediated-calpain-activation-pathway
#7
Meei-Ling Sheu, Chin-Chang Shen, Yuan-Siao Chen, Chih-Kang Chiang
Ochratoxin A (OTA) contaminated food increases reactive oxygen species (ROS) production in glomerulus and causes glomerulopathy. The molecular mechanisms still remain uncertain. In this study, we used mouse and rat glomerular mesangial cells and delineate the signaling pathway behind the OTA-triggered cell apoptosis. OTA dose-dependently induced expression of ER stress markers including phospho-PERK, phospho-eIF2α, GRP78, GRP94, and CHOP. Apoptosis events including cleavage of caspase-12, caspase-7, and PARP are also observed...
December 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/28029129/modeling-growth-and-toxin-production-of-toxigenic-fungi-signaled-in-cheese-under-different-temperature-and-water-activity-regimes
#8
Marco Camardo Leggieri, Simone Decontardi, Terenzio Bertuzzi, Amedeo Pietri, Paola Battilani
The aim of this study was to investigate in vitro and model the effect of temperature (T) and water activity (aw) conditions on growth and toxin production by some toxigenic fungi signaled in cheese. Aspergillus versicolor, Penicillium camemberti, P. citrinum, P. crustosum, P. nalgiovense, P. nordicum, P. roqueforti, P. verrucosum were considered they were grown under different T (0-40 °C) and aw (0.78-0.99) regimes. The highest relative growth occurred around 25 °C; all the fungi were very susceptible to aw and 0...
December 24, 2016: Toxins
https://www.readbyqxmd.com/read/28013544/sensitive-flow-through-immunoassay-for-rapid-multiplex-determination-of-cereal-borne-mycotoxins-in-feed-and-feed-ingredients
#9
Natalia V Beloglazova, Kinga Graniczkowska, Emmanuel Njumbe Ediage, Olga Averkieva, Sarah De Saeger
An easy-to-operate membrane-based flow-through test for multiplex screening of four mycotoxins (zearalenone, deoxynivalenol, aflatoxin B1, and ochratoxin A) in a variety of cereal based feed ingredients and compound feed, such as wheat, barley, soybean, wheat bran, rice, rice bran, maize, rapeseed meal, sunflower meal and various types of complete feed (ducklings feed, swine feed, broiler feed, piglet feed) was developed and validated. First, the antibodies were evaluated by enzyme-linked immunosorbent assay, then employed in the membrane rapid test...
December 25, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27983809/a-collaborative-study-determination-of-mycotoxins-in-corn-peanut-butter-and-wheat-flour-using-stable-isotope-dilution-assay-sida-and-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms
#10
Kai Zhang, Matthew R Schaab, Gavin Southwood, Elizabeth R Tor, Linda S Aston, Wenlu Song, Brian Eitzer, Sanghamitra Majumdar, Theodore Lapainis, Huy Mai, Kevin Tran, Aref El-Demerdash, Victor Vega, Yanxuan Cai, Jon W Wong, Alexandra J Krynitsky, Timothy H Begley
A collaborative study was conducted to evaluate stable isotope dilution assay (SIDA) and LC-MS/MS for the simultaneous determination of aflatoxins B1, B2, G1, and G2; deoxynivalenol; fumonisins B1, B2, and B3; ochratoxin A; HT-2 toxin; T-2 toxin; and zearalenone in foods. Samples were fortified with 12 (13)C uniformly labeled mycotoxins ((13)C-IS) corresponding to the native mycotoxins and extracted with acetonitrile/water (50:50 v/v), followed by centrifugation, filtration, and LC-MS/MS analysis. In addition to certified reference materials, the six participating laboratories analyzed corn, peanut butter, and wheat flour fortified with the 12 mycotoxins at concentrations ranging from 1...
January 3, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27983637/limited-link-between-oxidative-stress-and-ochratoxin-a-induced-renal-injury-in-an-acute-toxicity-rat-model
#11
Liye Zhu, Tao Yu, Xiaozhe Qi, Jing Gao, Kunlun Huang, Xiaoyun He, Haoshu Luo, Wentao Xu
Ochratoxin A (OTA) displays nephrotoxicity and hepatotoxicity. However, in the acute toxicity rat model, there is no evidence on the relationship between OTA and nephrotoxicity and hepatotoxicity. Based on this, the integrated analysis of physiological status, damage biomarkers, oxidative stress, and DNA damage were performed. After OTA treatment, the body weight decreased and AST, ALP, TP, and BUN levels in serum increased. Hydropic degeneration, swelling, vacuolization, and partial drop occurred in proximal tubule epithelial cells...
December 14, 2016: Toxins
https://www.readbyqxmd.com/read/27979281/multiclonal-plastic-antibodies-for-selective-aflatoxin-extraction-from-food-samples
#12
Engin Bayram, Erkut Yılmaz, Lokman Uzun, Rıdvan Say, Adil Denizli
Herein, we focused on developing a new generation of monolithic columns for extracting aflatoxin from real food samples by combining the superior features of molecularly imprinted polymers and cryogels. To accomplish this, we designed multiclonal plastic antibodies through simultaneous imprinting of aflatoxin subtypes B1, B2, G1, and G2. We applied Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and spectrofluorimetry to characterize the materials, and conducted selectivity studies using ochratoxin A and aflatoxin M1 (a metabolite of aflatoxin B1), as well as other aflatoxins, under competitive conditions...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27979197/potential-health-benefits-and-quality-of-dried-fruits-goji-fruits-cranberries-and-raisins
#13
Magdalena Jeszka-Skowron, Agnieszka Zgoła-Grześkowiak, Ewa Stanisz, Agnieszka Waśkiewicz
Dried fruits are important snacks and additives to other foods due to their taste and nutritional advantages. Therefore there is an important goal to control the quality of the food on the market for consumer's safety. Antioxidant activity of goji fruits (Lycium barbarum), cranberries (Vaccinium macrocarpon and oxycoccus) and raisins (Vitis vinifera) were studied using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and Folin-Ciocalteu assays. Cu, Mn and Ge influencing antioxidant activity were determined together with selected toxic metals (Cd, Ni and Pb)...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27976878/worldwide-occurrence-of-mycotoxins-in-cereals-and-cereal-derived-food-products-public-health-perspectives-of-their-co-occurrence
#14
Hyun Jung Lee, Dojin Ryu
Cereal grains and their processed food products are frequently contaminated with mycotoxins. Among many, five major mycotoxins of aflatoxins, ochratoxins, fumonisins, deoxynivalenol, and zearalenone are of significant public health concern as they can cause adverse effects in human. Being airborne or soilborne, cosmopolitan nature of the mycotoxigenic fungi contribute to the worldwide occurrence of mycotoxins. Based on the global occurrence data reported during the last ten years, the incidences and maximum levels in raw cereal grains were 55% and 1,642 µg/kg for aflatoxins, 29% and 1,164 µg/kg for ochratoxin A, 61% and 71,121 µg/kg for fumonisins, 58% and 41,157 µg/kg, for deoxynivalenol, and 46% and 3,049 µg/kg for zearalenone...
December 15, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27960261/a-concise-history-of-mycotoxin-research
#15
John I Pitt, J David Miller
Toxigenic fungi and mycotoxins entered human food supplies about the time when mankind first began to cultivate crops and to store them from one season to the next, perhaps 10,000 years ago. The storage of cereals probably initiated the transition by mankind from hunter-gatherer to cultivator, at the same time providing a vast new ecological niche for fungi pathogenic on grain crops or saprophytic on harvested grain, many of which produced mycotoxins. Grains have always been the major source of mycotoxins in the diet of man and his domestic animals...
December 27, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27959549/a-polyketide-synthase-encoded-by-the-gene-an15g07920-is-involved-in-the-biosynthesis-of-ochratoxin-a-in-aspergillus-niger
#16
Jian Zhang, Liuyang Zhu, Haoyu Chen, Min Li, Xiaojuan Zhu, Qiang Gao, Depei Wang, Ying Zhang
The polyketide synthase gene An15g07920 was known in Aspergillus niger CBS 513.88 as putatively involved in the production of ochratoxin A (OTA). Genome resequencing analysis revealed that the gene An15g07920 is also present in the ochratoxin-producing A. niger strain 1062. Disruption of An15g07920 in A. niger 1062 removed its capacity to biosynthesize ochratoxin β (OTβ), ochratoxin α (OTα), and OTA. These results indicate that the polyketide synthase encoded by An15g07920 is a crucial player in the biosynthesis of OTA, in the pathway prior to the phenylalanine ligation step...
December 28, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27942789/evaluation-of-the-automated-microflow-%C3%A2-and-metafer%C3%A2-platforms-for-high-throughput-micronucleus-scoring-and-dose-response-analysis-in-human-lymphoblastoid-tk6-cells
#17
Jatin R Verma, Benjamin J Rees, Eleanor C Wilde, Catherine A Thornton, Gareth J S Jenkins, Shareen H Doak, George E Johnson
The use of manual microscopy for the scoring of chromosome damage in the in vitro micronucleus assay is often associated with user subjectivity. This level of subjectivity can be reduced by using automated platforms, which have added value of faster with high-throughput and multi-endpoint capabilities. However, there is a need to assess the reproducibility and sensitivity of these automated platforms compared with the gold standard of the manual scoring. The automated flow cytometry-based MicroFlow(®) and image analysis-based Metafer™ were used for dose response analyses in human lymphoblastoid TK6 cells exposed to the model clastogen, methyl methanesulfonate (MMS), aneugen, carbendazim, and the weak genotoxic carcinogen, ochratoxin A (OTA)...
December 10, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27941297/serum-levels-of-ochratoxin-a-in-dogs-with-chronic-kidney-disease-ckd-a-retrospective-study
#18
Meucci Valentina, Luci Giacomo, Vanni Michele, Guidi Grazia, Perondi Francesca, Intorre Luigi
Ochratoxin A (OTA) is a mycotoxin produced by secondary metabolism of several fungi belonging to the genera Aspergillus and Penicillium. OTA is potentially nephrotoxic, neurotoxic, immunotoxic and carcinogenic in several animal species and in humans. This toxin has been detected in several human food and animal feed. The aim of this study was to determine OTA in blood samples of healthy and affected by chronic kidney disease (CKD) dogs. CKD group showed higher incidence of OTA-positivity than healthy dogs (96% vs...
December 12, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27924714/biomonitoring-of-mycotoxins-in-urine-pilot-study-in-mill-workers
#19
Wolfram Föllmann, Nurshad Ali, Meinolf Blaszkewicz, Gisela H Degen
Contamination of grains with mycotoxins results in a dietary background exposure of the general population. In occupational settings such as during processing of raw materials as in milling, an additional mycotoxin exposure by inhalation is possible. Biomonitoring is an integrative approach to assess human exposure from various sources and by all routes. To investigate possible workplace exposure to mycotoxins, a pilot study was conducted that compared levels of urinary biomarkers in mill workers to those in a control group with dietary mycotoxin intake alone...
2016: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/27924549/identification-of-ochratoxin-a-producing-black-aspergilli-from-grapes-using-loop-mediated-isothermal-amplification-lamp-assays
#20
Michelangelo Storari, Giovanni A L Broggini
The loop-mediated isothermal amplification (LAMP) allows the rapid and specific amplification of target DNA under isothermal conditions without a prior DNA purification step. Moreover, successful amplifications can be directly evaluated through a color change of the reaction solutions. Here, we describe two LAMP assays for the detection of ochratoxin-A producing black aspergilli isolated from grapes. The two assays can detect DNA of OTA-producing black aspergilli following a very simple sample preparation and have the potential to significantly speed up the routine monitoring of these toxigenic molds in vineyards...
2017: Methods in Molecular Biology
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