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Ochratoxin

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https://www.readbyqxmd.com/read/28445738/ochratoxin-a-and-its-metabolites-in-urines-of-german-adults-an-assessment-of-variables-in-biomarker-analysis
#1
Nurshad Ali, Katherine Muñoz, Gisela H Degen
Ochratoxin A (OTA), a mycotoxin known for its nephrotoxic and carcinogenic properties, is a worldwide occurring contaminant in a variety of food commodities. Biomonitoring (i.e. analysis in biological fluids) can serve to assess human internal exposure from all consumed foods and beverages. We now determined the concentration of OTA and its metabolite ochratoxin alpha (OTα) in plasma and in urine of two male volunteers with different food habits, in order to assess intra-individual temporal fluctuations and inter-individual differences in their biomarker levels...
April 23, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28432760/determination-of-ochratoxin-a-in-black-and-white-pepper-nutmeg-spice-mix-cocoa-and-drinking-chocolate-by-high-performance-liquid-chromatography-coupled-with-fluorescence-detection-collaborative-study
#2
Elena Cubero-Leon, Katrien Bouten, Hamide Senyuva, Joerg Stroka
A method validation study for the determination of ochratoxin A in black and white pepper (Piper spp.), nutmeg (Myristica fragrans), spice mix (blend of ginger, turmeric, pepper, nutmeg, and chili), cocoa powder, and drinking chocolate was conducted according to the International Harmonized Protocol of the International Union of Pure and Applied Chemistry. The method is based on the extraction of samples with aqueous methanol, followed by a cleanup of the extract with an immunoaffinity column. The determination is carried out by reversed-phase LC coupled with a fluorescence detector...
April 22, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28430148/a-rapid-magnetic-solid-phase-extraction-method-followed-by-liquid-chromatography-tandem-mass-spectrometry-analysis-for-the-determination-of-mycotoxins-in-cereals
#3
Giorgia La Barbera, Anna Laura Capriotti, Chiara Cavaliere, Patrizia Foglia, Carmela Maria Montone, Riccardo Zenezini Chiozzi, Aldo Laganà
Mycotoxins can contaminate various food commodities, including cereals. Moreover, mycotoxins of different classes can co-contaminate food, increasing human health risk. Several analytical methods have been published in the literature dealing with mycotoxins determination in cereals. Nevertheless, in the present work, the aim was to propose an easy and effective system for the extraction of six of the main mycotoxins from corn meal and durum wheat flour, i.e., the main four aflatoxins, ochratoxin A, and the mycoestrogen zearalenone...
April 21, 2017: Toxins
https://www.readbyqxmd.com/read/28430142/initial-spore-density-has-an-influence-on-ochratoxin-a-content-in-aspergillus-ochraceus-cgmcc-3-4412-in-pdb-and-its-interaction-with-seeds
#4
Caiyan Li, Yanmin Song, Lu Xiong, Kunlun Huang, Zhihong Liang
The morphology and secondary metabolism of Aspergillus spp. are associated with initial spore density (ISD). Fatty acids (FA) are involved in the biosynthesis of aflatoxins (AF) through Aspergillus quorum sensing (QS). Here, we studied how ochratoxin A (OTA) was regulated by spore density in Aspergillus ochraceus CGMCC 3.4412. The results contribute to understanding the role of spore density in morphogenesis, OTA biosynthesis, and host-pathogen interactions. When A. ochraceus was grown in Potato Dextrose Broth (PDB) media at different spore densities (from 10¹ to 10⁶ spores/mL), more OTA was produced when ISD were increased, but a higher level of ISD inhibited OTA biosynthesis...
April 21, 2017: Toxins
https://www.readbyqxmd.com/read/28422066/diabetogenic-effects-of-ochratoxin-a-in-female-rats
#5
Firdevs Mor, Omur Sengul, Senay Topsakal, Mehmet Akif Kilic, Ozlem Ozmen
In this study, the diabetogenic effects of long term Ochratoxin A (OTA) administration in rats were investigated, and its role in the etiology of diabetes mellitus (DM) was examined utilizing 42 female Wistar rats for these purposes. The rats were divided into three different study and control groups according to the duration of the OTA administration. The rats received 45 μg OTA daily in their feed for 6, 9 and 24 weeks, respectively. Three control groups were also used for the same time periods. Blood and pancreatic tissue samples were collected during the necropsy at the end of the 6, 9 and 24 weeks...
April 19, 2017: Toxins
https://www.readbyqxmd.com/read/28401806/quantification-of-mycotoxins-in-vegetable-oil-by-uplc-ms-ms-after-magnetic-solid-phase-extraction
#6
Yan Zhao, Li-Hong Wan, Xiao-Lin Bai, Yi-Ming Liu, Feng-Ping Zhang, Yao-Min Liu, Xun Liao
Detection of mycotoxin contamination in foodstuffs is highly significant for public health. Herein we report an analytical method based on magnetic solid phase extraction (MSPE) and UPLC-MS/MS for simultaneous determination of mycotoxins, including fumonisins B1 (FB1), zearalenone (ZON) and ochratoxin A (OTA) in vegetable oil. Magnetic nanoparticles coated with double layers of silicon dioxide were synthesized and found to be an effective MSPE adsorbent for mycotoxins. The proposed MSPE procedure serves not only for sample cleanup, but also for mycotoxin enrichment that enhances greatly the assay sensitivity...
April 12, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28387467/cetylpyridinium-chloride-functionalized-silica-coated-magnetite-microspheres-for-the-solid-phase-extraction-and-pre-concentration-of-ochratoxin-a-from-environmental-water-samples-with-high-performance-liquid-chromatographic-analysis
#7
Meihua Hu, Pengcheng Huang, Lili Suo, Fangying Wu
A new method based on cetylpyridinium chloride coated ferroferric oxide/silica magnetic microspheres as an efficient solid-phase adsorbent was developed for the extraction and enrichment of ochratoxin A. The determination of ochratoxin A was obtained by high-performance liquid chromatography with fluorescence detection. In the presence of cetylpyridinium chloride, the adsorption capacity of ferroferric oxide/silica microspheres was 5.95 mg/g for ochratoxin A. The experimental parameters were optimized, including the amounts of ferroferric oxide/silica microspheres (20 mg) and cetylpyridinium chloride (0...
April 7, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28380344/mycotoxin-risk-assessment-for-consumers-of-groundnut-in-domestic-markets-in-nigeria
#8
Oluwawapelumi A Oyedele, Chibundu N Ezekiel, Michael Sulyok, Modupeade C Adetunji, Benedikt Warth, Olusegun O Atanda, Rudolf Krska
The fungal and multi-mycotoxin profiles of groundnuts sold in domestic markets in Nigeria as well as the associated risk to consumers were assessed in the present study. Four hundred fungal isolates representing mainly Aspergillus [58.6%: Aspergillus section Flavi (37.1%) and A. niger-clade (21.5%)], Penicillium (40.9%) and Fusarium (0.5%) were isolated from 82 (97.6%, n=84) groundnut samples collected from four agro-ecological zones (AEZs) of Nigeria. The incidence of aflatoxin-producing A. flavus isolates (71%) was significantly (p<0...
March 28, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28376398/adsorption-of-aflatoxin-b1-zearalenone-and-ochratoxin-a-by-microorganisms-isolated-from-kefir-grains
#9
Fadia Ben Taheur, Kais Fedhila, Kamel Chaieb, Bochra Kouidhi, Amina Bakhrouf, Luís Abrunhosa
A strategy to reduce the deleterious effects of mycotoxins is to use dietary supplements that contain microorganisms that bind mycotoxins and decrease their gastrointestinal absorption. Novel strains were isolated from a Kefir culture and assessed for their mycotoxin adsorption and biotransformation ability. The most active strains were identified using DNA sequencing, and the stability of microorganism/mycotoxin complexes was evaluated using buffer solutions to simulate the pH conditions in the gastrointestinal tract...
March 28, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28372187/determination-of-mycotoxins-in-plant-based-beverages-using-quechers-and-liquid-chromatography-tandem-mass-spectrometry
#10
Eugènia Miró-Abella, Pol Herrero, Núria Canela, Lluís Arola, Francesc Borrull, Rosa Ras, Núria Fontanals
A method was developed for the simultaneous determination of 11 mycotoxins in plant-based beverage matrices, using a QuEChERS extraction followed by ultra-high performance liquid chromatography coupled to tandem mass spectrometry detection (UHPLC-(ESI)MS/MS). This multi-mycotoxin method was applied to analyse plant-based beverages such as soy, oat and rice. QuEChERS extraction was applied obtaining suitable extraction recoveries between 80 and 91%, and good repeatability and reproducibility values. Method Quantification Limits were between 0...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28347757/tds-exposure-project-how-and-when-to-consider-seasonalityin-a-total-diet-study
#11
Chabi Fabrice Elegbede, Alexandra Papadopoulos, Anna Elena Kolbaum, Aida Turrini, Lorenza Mistura, Oliver Lindtner, Véronique Sirot
Seasonality is a key issue of total diet studies (TDS), as season may impact consumption patterns and concentrations of food chemicals, then exposure levels. This work aimed at analyzing the impact on dietary exposure of different options of seasonality management, to propose guidelines for TDS sampling. Dietary exposure to nine chemicals was assessed for adults and children from the second French Individual and National Food Consumption Survey: arsenic, copper, manganese, dioxins, furans, dioxin-like and non dioxin-like PCBs, deoxinivalenol and ochratoxin A...
March 27, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28342297/high-performance-liquid-chromatographic-determination-of-multi-mycotoxin-in-cereals-and-bean-foodstuffs-using-interference-removal-solid-phase-extraction-combined-with-optimized-dispersive-liquid-liquid-microextraction
#12
Jian Zhou, Jiao-Jiao Xu, Bai-Fen Huang, Zeng-Xuan Cai, Yi-Ping Ren
A novel pre-treatment was proposed for the simultaneous determination of aflatoxins, ochratoxin A and zearalenone in foodstuffs using high-performance liquid chromatography with fluorescence detection. The analytical procedure was based on a first step using a quick, easy, cheap, effective, rugged, and safe based extraction procedure, followed by salting out and purification with a C18 solid-phase extraction column as interference removal clean-up. Subsequently, collected supernatant was subjected to dispersive liquid-liquid microextraction...
March 25, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28334872/hplc-dad-fld-method-for-simultaneous-determination-of-mycotoxins-in-wheat-bran
#13
Maria N Irakli, Adriana Skendi, Maria D Papageorgiou
Aflatoxins, deoxynivalenol, ochratoxin A and zearalenone are the most important mycotoxins that everyone on its own, in groups or simultaneously contaminate cereals. The external layers of cereal grains (bran) apart from health promoting ingredients are also the most contaminated part with reference to mycotoxin's presence. Therefore, consumption of a high fiber wheat-based diet represent an increased risk to consumer's health. The objective of this study was to develop a simple and reliable high performance liquid chromatography method for the simultaneous determination of these mycotoxins in wheat bran (WB)...
March 18, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28334625/magnetic-microspheres-based-cytometric-bead-array-assay-for-highly-sensitive-detection-of-ochratoxin-a
#14
Weijun Kong, Changbin Xiao, Guangyao Ying, Xiaofei Liu, Xiaohong Zhao, Ruilin Wang, Li Wan, Meihua Yang
Accurate and sensitive quantification of a specific class of mycotoxins at trace levels in complex matrices with greener approaches is of significant importance. In this study, a green and economical protocol of magnetic microspheres-based cytometric bead array (CBA) assay on indirect competitive principle was developed for sensitive and rapid detection of ochratoxin A (OTA) in malts with a small number of standard and sample solutions. The protocol included the competition of OTA in malt samples and that covalently coupled on the surface of microspheres with its monoclonal antibodies, the separation and aggregation of the magnetic microspheres, and the fluorescence detection of fluorescein isothiocyanate labeled goat anti-mouse immunoglobulin G probes...
March 14, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28333080/a-review-epigenetic-mechanism-in-ochratoxin-a-toxicity-studies
#15
REVIEW
Liye Zhu, Boyang Zhang, Yaqi Dai, Hongyu Li, Wentao Xu
Ochratoxin A (OTA) is a natural contaminant that has displayed nephrotoxicity and hepatotoxicity in mammals. It contaminates a great variety of foodstuffs and threatens people's lives. The molecular mechanism of OTA-induced toxicity has been studied since 1965. Moreover, epigenetic mechanisms are also studied in OTA-induced toxicity. Additionally, the mode of OTA epigenetic research has been advanced in research hotspots. However, there is still no epigenetic study of OTA-induced toxicity. In this review, we discuss the relationship between these epigenetic mechanisms and OTA-induced toxicity...
March 23, 2017: Toxins
https://www.readbyqxmd.com/read/28332414/determination-of-mycotoxins-in-biscuits-dried-fruits-and-fruit-jams-assessment-of-human-exposure
#16
Biljana Škrbić, Igor Antić, Jelena Cvejanov
A reliable, fast and simple method using UHPLC-MS/MS was developed for the determination of aflatoxins B1 (AFB1), G1 (AFG1), B2 (AFB2) and G2 (AFg2), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEA), HT-2 toxin and T-2 toxin in crude extracts of biscuits with fruit filling, cookies, dried fruits and fruit jams. The method was successfully demonstrated on 39 samples of biscuits with fruit filling, 34 cookies, 14 dried fruits and 10 fruit jams. The mycotoxins detected in biscuits samples were ZEA, OTA, T-2, and AFB1 with average concentration of positive samples of 2...
March 23, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28322500/liquid-chromatography-with-mass-spectrometry-analysis-of-mycotoxins-in-food-samples-using-silica-hydride-based-stationary-phases
#17
Joseph P Pesek, Maria T Matyska, Jessica F Hoffmann, Nathalia A Madruga, Rosane L Crizel, Moacir C Elias, Nathan L Vanier, Fabio C Chaves
Liquid chromatography with mass spectrometry analysis of selected food samples using silica hydride stationary phases allowed for the identification and quantification of common mycotoxins including aflatoxin B1, B2, G1, G2, ochratoxin A, and fumosinin B1. Phenyl and C18 columns showed relatively similar selectivity based on hydrophobicity but the phenyl phase provides and additional mechanism, π-π interaction. The most hydrophobic of the analyzed compounds was more strongly retained on the C18 column and also has fewer unsaturated sites, which limited the interaction with the phenyl phase...
March 21, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28318567/determination-of-ochratoxin-a-in-wine-by-packed-in-tube-solid-phase-microextraction-followed-by-high-performance-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#18
Mariane A Andrade, Fernando M Lanças
Ochratoxin A (OTA), a widely studied mycotoxin, can be found in a variety of food matrices. As its concentration in food is generally low (in the order of μg kg(-1)), sample preparation techniques are necessary for the analyte purification and pre-concentration in order to achieve the required low detection limits. The separation and detection methods used for OTA analysis should also offer proper sensitivity in order to allow the adequate quantification of the analyte. This manuscript addresses the development of a methodology aiming the analysis of OTA in wine samples by packed in-tube SPME in flow through extraction mode coupled to HPLC-MS/MS...
April 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28300780/simultaneous-determination-of-multi-mycotoxins-in-cereal-grains-collected-from-south-korea-by-lc-ms-ms
#19
Dong-Ho Kim, Sung-Yong Hong, Jea Woo Kang, Sung Min Cho, Kyu Ri Lee, Tae Kyung An, Chan Lee, Soo Hyun Chung
An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B₁, aflatoxin B₂, aflatoxin G₁, aflatoxin G₂, fumonisin B₁, fumonisin B₂, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass spectrometry (LC/MS/MS) after a single immunoaffinity column clean-up. The method showed a good linearity, sensitivity, specificity, and accuracy in mycotoxin determination by LC/MS/MS...
March 16, 2017: Toxins
https://www.readbyqxmd.com/read/28299415/multi-mycotoxin-analysis-using-dried-blood-spots-and-dried-serum-spots
#20
Bernd Osteresch, Susana Viegas, Benedikt Cramer, Hans-Ulrich Humpf
In this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DON-3-GlcA; T-2; HT-2; and HT-2-4-GlcA), fumonisin B1 (FB1), ochratoxins (OTA and its thermal degradation product 2'R-OTA; OTα; 10-hydroxychratoxin A, 10-OH-OTA), citrinin (CIT and its urinary metabolite dihydrocitrinone, DH-CIT), zearalenone and zearalanone (ZEN, ZAN), altenuene (ALT), alternariols (AOH; alternariol monomethyl ether, AME), enniatins (EnA, EnA1, EnB, EnB1) and beauvericin (Bea) was validated for two matrices, serum (DSS), and whole blood (DBS)...
March 15, 2017: Analytical and Bioanalytical Chemistry
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