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https://www.readbyqxmd.com/read/28651425/expression-of-gfr%C3%AE-3-correlates-with-tumor-progression-and-promotes-cell-metastasis-in-urothelial-carcinoma
#1
Yang Yan, Meng-Nan Liu, Bin Yang, Jiang Geng, Jun-Hua Zheng, Xu-Dong Yao
BACKGROUND: Urothelial carcinoma (UC) is a major heath problem in the general population. We aimed to evaluate the function of GFRα3 and unravel its underlying molecular mechanism to develop novel treatment options equivalent to UC. METHODS: To evaluate the function of GFRα3, a group of 60 pairs of UC patients were recruited in for this study. UC tissues and their adjacent normal control tissues (NCTs) were collected between 2012 and 2015. We used immunohistochemistry to analyze the correlation between GFRα3 expression and clinicopathologic variables and patient survival...
June 23, 2017: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/28650862/the-potential-combinational-effect-of-mir-34a-with-celecoxib-in-osteosarcoma
#2
Xia Chen, Dan Peng, Yi Shen, Bo Liu, Hongkang Zhou, Huai Tao, Jufang Huang
Advanced osteosarcoma (OS) is usually treated by preoperative and postoperative chemotherapy, but there are a very limited number of active agents. Celecoxib (Cel) is a COX-2-selective nonsteroidal anti-inflammatory drug and its antitumoral effect has been shown widely in a variety of cancers including OS cells in vitro. However, the potential combinational effect of Cel with other biological therapy has not been reported in OS cells. In this study, the effects of Cel, miR-34a mimics, and their combination on cell proliferation (MTT assay), migration (in-vitro scratch assay), invasion (transwell assay), mRNA (real-time PCR), and protein (Western blot) expression of associated signal transductions were investigated in cultured MG63 cells...
June 23, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/28647190/matrine-inhibits-bladder-cancer-cell-growth-and-invasion-in%C3%A2-vitro-through-pi3k-akt-signaling-pathway-an-experimental-study
#3
Yu Yang, Jia-Xiang Guo, Zhi-Qiang Shao, Jiang-Ping Gao
OBJECTIVE: To study the inhibitory effect of matrine on bladder cancer cell growth and invasion in vitro through PI3K/AKT signaling pathway. METHODS: Human T24 bladder cancer cell lines were cultured and treated with different doses of matrine (0.25 mg/mL, 0.5 mg/mL and 1.0 mg/mL) as well as 20 μmol/L PI3K inhibitor LY294002 for 24 h, and the cell proliferation activity, the number of invasive cells as well as the expression of p-PI3K, p-AKT, proliferation genes and invasion genes were determined...
May 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28646881/heme-molecule-functions-as-an-endogenous-agonist-of-astrocyte-tlr2-to-contribute-to-secondary-brain-damage-after-intracerebral-hemorrhage
#4
Hyunjung Min, Boomin Choi, Yong Ho Jang, Ik-Hyun Cho, Sung Joong Lee
Toll-like receptor 2 (TLR2) was recently shown to contribute to secondary brain damage after intracerebral hemorrhage (ICH), although the molecular mechanisms of this contribution are elusive. In this study, we tested the hypothesis that hemin functions as a TLR2 endogenous agonist, causing proinflammatory astrocyte activation and secondary brain damage after ICH. Hemin administration to the mouse brain striatum induced ICH injury and neurological deficits, however, the brain injury volume and neurological deficits due to hemin injection were significantly reduced in TLR2 knock-out (KO) mice...
June 24, 2017: Molecular Brain
https://www.readbyqxmd.com/read/28645189/the-potential-role-of-retinoic-acid-receptor-%C3%AE-on-glomerulosclerosis-in-rats-and-podocytes-injury-is-associated-with-the-induction-of-mmp2-and-mmp9
#5
Xiuping Chen, Yuanhan Qin, Tianbiao Zhou, Ling Jiang, Fengying Lei, He Qin, Lei Zhang, Zhiqiang Zhou
Retinoic acid receptor α (RARα) plays a crucial role in kidney disease. However, the underlying mechanisms in glomerulosclerosis (GS) is still not clear. The roles of RARα in an adriamycin (ADR)-induced GS rat model and in ADR-induced podocyte injury in vitro were investigated. RARα was over-expressed in GS rats, and serum, urine and kidney samples were collected to detect the induction of the expression of the receptor. RARα expression was inhibited and/or over-expressed in cultured podocytes following injury, as demonstrated by morphometric assays, cell toxicity, and matrix metalloproteinase (MMP) enzymatic activity...
June 22, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28644870/effects-of-nicotine-on-corneal-wound-healing-following-acute-alkali-burn
#6
Jong Won Kim, Chae Woong Lim, Bumseok Kim
Epidemiological studies have indicated that smoking is a pivotal risk factor for the progression of several chronic diseases. Nicotine, the addictive component of cigarettes, has powerful pathophysiological properties in the body. Although the effects of cigarette smoking on corneal re-epithelialization have been studied, the effects of nicotine on corneal wound healing-related neovascularization and fibrosis have not been fully demonstrated. The aim of this study was to evaluate the effects of chronic administration of nicotine on corneal wound healing following acute insult induced by an alkali burn...
2017: PloS One
https://www.readbyqxmd.com/read/28642677/a-novel-workflow-combining-plaque-imaging-plaque-and-plasma-proteomics-identifies-biomarkers-of-human-coronary-atherosclerotic-plaque-disruption
#7
Regent Lee, Roman Fischer, Philip D Charles, David Adlam, Alessandro Valli, Katalin Di Gleria, Rajesh K Kharbanda, Robin P Choudhury, Charalambos Antoniades, Benedikt M Kessler, Keith M Channon
BACKGROUND: Atherosclerotic plaque rupture is the culprit event which underpins most acute vascular syndromes such as acute myocardial infarction. Novel biomarkers of plaque rupture could improve biological understanding and clinical management of patients presenting with possible acute vascular syndromes but such biomarker(s) remain elusive. Investigation of biomarkers in the context of de novo plaque rupture in humans is confounded by the inability to attribute the plaque rupture as the source of biomarker release, as plaque ruptures are typically associated with prompt down-stream events of myocardial necrosis and systemic inflammation...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28641616/-effect-of-lpxn-overexpression-on-the-proliferation-adhesion-and-invasion-of-thp-1-cells-and-its-mechamisms
#8
Hai-Ping Dai, Guo-Hua Zhu, Li-Li Wu, Qian Wang, Hong Yao, Qin-Rong Wang, Li-Jun Wen, Hui-Ying Qiu, Qun Shen, Su-Ning Chen, De-Pei Wu
OBJECTIVE: To explore the effect of LPXN overexpression on the proliferation, adhesion and invasion of THP-1 cells and its possible mechanism. METHODS: A THP-1 cell line with stable overexpression of LPXN was constucted by using a lentivirus method, CCK-8 was used to detect the proliferation of cells, adhesion test was used to evaluate adhesion ablity of cells to Fn. Transwell assay was used to detect the change of invasion capability. Western blot was used to detect expression of LPXN, ERK, pERK and integrin α4, α5, β1, the Gelatin zymography was applied to detect activity of MMP2/MMP9 secreted by the THP-1 cells...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28638138/immune-regulation-by-oral-tolerance-induces-alternate-activation-of-macrophages-and-reduces-markers-of-plaque-destabilization-in-apob-tm2sgy-ldlr-tm1her-j-mice
#9
Lakshmi Narasimha Thota, Thiruvelselvan Ponnusamy, Sheena Philip, Xinjie Lu, Lakshmi Mundkur
Atherosclerosis is the leading cause for cardiovascular mortality. We determined the effect of multi-antigenic construct expressing three peptides AHC (ApoB100, HSP60 and outer membrane protein of chlamydia pneumonia) in stabilizing advanced atherosclerosis in Apob(tm2Sgy)/Ldlr(tm1Her/J) mice. Atherosclerosis was induced by feeding high fat diet (HFD) to mice for 10 weeks, followed by five oral dosing with purified AHC or ovalbumin on alternate days and continued on HFD for another 10 weeks. Tolerance was associated with significantly higher numbers of regulatory T cells both in aortic sinus and spleen with higher mRNA expression of CTLA4 (3 fold), Foxp3 (1...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28633482/markers-of-adipogenesis-but-not-inflammation-in-adipose-tissue-are-independently-related-to-insulin-sensitivity
#10
Natalia Matulewicz, Magdalena Stefanowicz, Agnieszka Nikolajuk, Monika Karczewska-Kupczewska
Context: In obesity, adipose tissue (AT) undergoes dynamic remodeling, including an alternations in adipogenesis, AT-resident cell content, angiogenesis and turnover of extracellular matrix (ECM) components. Studies on AT in humans have been carried out mostly on people with severe metabolic abnormalities, like type 2 diabetes or morbid obesity. Objective: The purpose of the present study was to investigate subcutaneous AT gene expression of markers of adipogenesis, ECM remodeling and inflammation in young, healthy, overweight or obese subjects...
June 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28632489/the-effects-of-platelet-gel-on-cultured-human-retinal-pigment-epithelial-cells
#11
Sahar Balagholi, Shaban Alizadeh, Abouzar Bagheri, Yashar Amizadeh, Mozhgan Rezaei Kanavi
The positive role of platelet gel (PG) in tissue regeneration is well known, however, other characteristics of PG still remain to be determined. We investigated cellular and molecular changes in cultured human retinal pigment epithelial (hRPE) cells when treated with different concentrations of PG named PG1, PG2, and PG3. hRPE cells were isolated from donor eyes of two newborn children, within 24 hours after their death. The cells were treated with three concentrations of PG for 7 days: 3 × 104/ml (PG1), 6 × 104/ml (PG2), and 9 × 104/ml (PG3)...
June 20, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28629038/electrospinning-of-pva-sericin-nanofiber-and-the-effect-on-epithelial-mesenchymal-transition-of-a549-cells
#12
Shanshan Yan, Xiuchun Li, Jing Dai, Yiqun Wang, Binbin Wang, Yi Lu, Jianlin Shi, Pengyu Huang, Jinkang Gong, Yuan Yao
This research aims to investigate the cell-nanomaterial interaction between epithelial-mesenchymal transition of A549 cell and electrospinning nanofibers composed of polyvinyl alcohol (PVA)/silk sericin (SS). The electrospinning of regenerated nanofiber was performed with water as a spinning solvent and glutaraldehyde as a chemical cross-linker. Solution concentration, applied voltage and spin distances as well as other parameters were optimized to generate fine nanofibers with smooth surface in good homogeneity...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628833/urtica-dioica-extract-suppresses-mir-21-and-metastasis-related-genes-in-breast-cancer
#13
Behzad Mansoori, Ali Mohammadi, Shahriar Hashemzadeh, Solmaz Shirjang, Ali Baradaran, Milad Asadi, Mohammad Amin Doustvandi, Behzad Baradaran
BACKGROUND: Breast cancer has a high prevalence among women worldwide. Tumor invasion and metastasis still remains an open issue that causes most of the therapeutic failures and remains the prime cause of patient mortality. Hence, there is an unmet need to develop the most effective therapeutic approach with the lowest side effects and highest cytotoxicity that will effectively arrest or eradicate metastasis. METHODS: An MTT assay and scratch test were used to assess the cytotoxicity and migration effects of Urtica dioica on the breast cancer cells...
June 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28627651/sox4-promotes-melanoma-cell-migration-and-invasion-though-the-activation-of-the-nf-%C3%AE%C2%BAb-signaling-pathway
#14
Qiong Cheng, Jinfeng Wu, Yaohua Zhang, Xiao Liu, Nan Xu, Fuguo Zuo, Jinhua Xu
SOX4 has been reported to be abnormally expressed in many types of cancer, including melanoma. However, its role in cell proliferation and metastasis remains controversial. In this study, SOX4 was downregulated or overexpressed in A375, A2058 and A875 melanoma cells by siRNA or lentivirus transfection, respectively. Cell metastasis was observed by Transwell assay. In an aim to elucidate the underlying mechanisms, we determined the expression of nuclear factor-κB (NF-κB) by real-time PCR assay and western blot analysis...
June 16, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28627627/stromal-antigen-2-functions-as-a-tumor-suppressor-in-bladder-cancer-cells
#15
Han Wang, Jianhua Zhong, Chenglong Wu, Yang Liu, Jinghua Zhang, Xiaowen Zou, Yan Chen, Jinfeng Su, Gang Yang, Yuantang Zhong, Aifa Tang
Stromal antigen 2 (STAG2) is a subunit of the cohesion complex that plays an important role in the normal segregation of sister chromatids during mitosis or meiosis. However, the effect of STAG2 on the bladder cancer cell proliferation, migration, and invasion has not yet been fully clarified. In this study, we aimed to characterize STAG2 expression and functional significance in BC and adjacent normal tissue. Notably, STAG2 expression was markedly lower in BC cells and tumor tissues than their normal counterparts at the gene and protein levels...
June 12, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28624794/knockdown-of-hdac1-expression-suppresses-invasion-and-induces-apoptosis-in-glioma-cells
#16
Xiao-Qiang Wang, Hong-Min Bai, Shi-Ting Li, Hui Sun, Ling-Zhao Min, Bang-Bao Tao, Jun Zhong, Bin Li
Glioma is the most common malignant tumor of the central nervous system, with a low survival rate of five years worldwide. Although high expression and prognostic value of histone deacetylase 1 (HDAC1) have been recently reported in various types of human tumors, the molecular mechanism underlying the biological function of HDAC1 in glioma is still unclear. We found that HDAC1 was elevated in glioma tissues and cell lines. HDAC1 expression was closely related with pathological grade and overall survival of patients with gliomas...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28624217/efficacy-of-postnatal-in%C3%A2-vivo-nonsense-suppression-therapy-in-a-pax6-mouse-model-of-aniridia
#17
Xia Wang, Kevin Gregory-Evans, Kishor M Wasan, Olena Sivak, Xianghong Shan, Cheryl Y Gregory-Evans
Nonsense mutations leading to premature stop codons are common occurring in approximately 12% of all human genetic diseases. Thus, pharmacological nonsense mutation suppression strategies would be beneficial to a large number of patients if the drugs could be targeted to the affected tissues at the appropriate time. Here, we used nonsense suppression to manipulate Pax6 dosage at different developmental times in the eye of the small eye (Pax6(Sey/+); G194X) mouse model of aniridia. Efficacy was assessed by functional assays for visual capacity, including electroretinography and optokinetic tracking (OKT), in addition to histological and biochemical studies...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28623956/differential-protein-expression-in-chicken-macrophages-and-heterophils-in-vivo-following-infection-with-salmonella-enteritidis
#18
Zuzana Sekelova, Hana Stepanova, Ondrej Polansky, Karolina Varmuzova, Marcela Faldynova, Radek Fedr, Ivan Rychlik, Lenka Vlasatikova
In this study we compared the proteomes of macrophages and heterophils isolated from the spleen 4 days after intravenous infection of chickens with Salmonella Enteritidis. Heterophils were characterized by expression of MMP9, MRP126, LECT2, CATHL1, CATHL2, CATHL3, LYG2, LYZ and RSFR. Macrophages specifically expressed receptor proteins, e.g. MRC1L, LRP1, LGALS1, LRPAP1 and a DMBT1L. Following infection, heterophils decreased ALB and FN1, and released MMP9 to enable their translocation to the site of infection...
June 17, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28621422/a-new-role-of-the-membrane-type-matrix-metalloproteinase-16-mmp16-mt3-mmp-in-neural-crest-cell-migration
#19
Lee Roth, Rotem Kalev-Altman, Efrat Monsonego-Ornan, Dalit Sela-Donenfeld
Neural crest cells (NCCs) are a transient population of neuroectodermal-originated cells that populate the dorsal neural tube (dNT), before migrating and giving rise to multiple cell lineages in the developing embryo. Prior to their migration, NCCs undergo epithelial-to-mesenchymal-transition (EMT) through which they lose cell contacts and detach from the dNT to invade their surrounding environment. Multiple signals and transcription factors have been identified to regulate these events. Yet, less is known regarding effectors that act downstream to execute the actual NCC separation and migration...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28621263/loss-of-barx1-promotes-hepatocellular-carcinoma-metastasis-through-up-regulating-mgat5-and-mmp9-expression-and-indicates-poor-prognosis
#20
Guodong Wang, Jian Liu, Yi Cai, Jie Chen, Wenbing Xie, Xiangqian Kong, Wenjie Huang, Hao Guo, Xiaodi Zhao, Yuanyuan Lu, Lu Niu, Xiaowei Li, Haijia Zhang, Chao Lei, Zhijie Lei, Jipeng Yin, Hao Hu, Fan Yu, Yongzhan Nie, Limin Xia, Kaichun Wu
Metastasis is the major dominant reason for poor prognosis of hepatocellular carcinoma (HCC) after surgical treatment. However, the molecular mechanism of metastasis has not been well characterzied. Here, we report a novel function of Barx homeobox1 (Barx1) in inhibiting HCC invasion and metastasis. Barx1 expression is significantly decreased in human HCC tissues than in adjacent non-tumorous tissues and normal liver tissues. Low Barx1 expression is correlated with higher tumor-nodule-metastasis stage and indicates poor prognosis...
May 30, 2017: Oncotarget
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