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https://www.readbyqxmd.com/read/29035824/knockdown-of-diaph1-expression-inhibits-migration-and-decreases-the-expression-of-mmp2-and-mmp9-in-human-glioma-cells
#1
Can Zhang, Lin Wang, Jiaying Chen, Jie Liang, Yaming Xu, Zhe Li, Fuxue Chen, Dongshu Du
As the most common primary central nervous system tumor, glioma is characterized by high levels of mortality and migration. Unclear boundary with normal brain tissue results in poor treatment. The mammalian diaphanous-related formin 1 (Diaph1) which belongs to formin-homology protein family, is a target of RhoA and involved in a number of actin-related biological processes, which abnormally expressed in pathological conditions in a number of tumors. Immunohistochemical analysis showed that Diaph1 was overexpressed in glioma tissues compared with normal human brain tissue...
October 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29035450/imbalance-between-matrix-metalloproteinases-and-tissue-inhibitors-of-metalloproteinases-promotes-invasion-and-metastasis-of-head-and-neck-squamous-cell-carcinoma
#2
Adina E Stanciu, Adina Zamfir-Chiru-Anton, Marcel M Stanciu, Cristian R Popescu, Dan C Gheorghe
BACKGROUND: The balance between collagenolysis and its inhibition is critical during extracellular matrix remodeling in head and neck squamous cell carcinoma (HNSCC). An imbalance between matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) plays a critical role in the development of tumor cell invasion and metastasis. The purpose of the study was to determine MMP-9/TIMP-1 and MMP-2/TIMP-2 ratios in the sera of patients with HNSCC in relation to clinicopathological parameters...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29026538/plasma-and-cerebrospinal-fluid-interleukin-1%C3%AE-during-lipopolysaccharide-induced-systemic-inflammation-in-ewes-implanted-or-not-with-slow-release-melatonin
#3
Janina Skipor, Marta Kowalewska, Aleksandra Szczepkowska, Anna Majewska, Tomasz Misztal, Marek Jalynski, Andrzej P Herman, Katarzyna Zabek
BACKGROUND: Interleukin-1β (IL-1β) is important mediator of inflammatory-induced suppression of reproductive axis at the hypothalamic level. At the beginning of inflammation, the main source of cytokines in the cerebrospinal fluid (CSF) is peripheral circulation, while over time, cytokines produced in the brain are more important. Melatonin has been shown to decrease pro-inflammatory cytokines concentration in the brain. In ewes, melatonin is used to advance the onset of a breading season...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29025423/downregulation-of-nmi-promotes-tumor-growth-and-predicts-poor-prognosis-in-human-lung-adenocarcinomas
#4
Jingshu Wang, Kun Zou, Xu Feng, Miao Chen, Cong Li, Ranran Tang, Yang Xuan, Meihua Luo, Wangbing Chen, Huijuan Qiu, Ge Qin, Yixin Li, Changlin Zhang, Binyi Xiao, Lan Kang, Tiebang Kang, Wenlin Huang, Xinfa Yu, Xiaojun Wu, Wuguo Deng
BACKGROUND: N-myc (and STAT) interactor (NMI) plays vital roles in tumor growth, progression, and metastasis. In this study, we identified NMI as a potential tumor suppressor in lung cancer and explored its molecular mechanism involved in lung cancer progression. METHODS: Human lung cancer cell lines and a mouse xenograft model was used to study the effect of NMI on tumor growth. The expression of NMI, COX-2 and relevant signaling proteins were examined by Western blot...
October 12, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/29024528/photobiomodulation-alters-matrix-protein-activity-in-stressed-fibroblast-cells-in-vitro
#5
S M Ayuk, H Abrahamse, N N Houreld
A balance is maintained between matrix synthesis and degradation, and a prolonged increase in matrix metalloproteinases (MMPs) affects healing. Photobiomodulation (PBM) speeds up healing and alters wound environment. The study aimed to determine changes in protein and gene expression of collagen type 1 (Col-I), MMP-3 and -9 and TIMP-1 in fibroblasts irradiated at 660 or 830 nm. Commercially purchased human skin fibroblast cells were modeled into five groups: namely, normal, normal wounded, diabetic wounded, hypoxic wounded and diabetic hypoxic wounded...
October 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29022011/placental-extract-ameliorates-non-alcoholic-steatohepatitis-nash-by-exerting-protective-effects-on-endothelial-cells
#6
Akihiro Yamauchi, Akiko Kamiyoshi, Teruhide Koyama, Nobuyoshi Iinuma, Shumpei Yamaguchi, Hiroyuki Miyazaki, Eiichi Hirano, Taiichi Kaku, Takayuki Shindo
Non-alcoholic steatohepatitis (NASH) is a severe form of fatty liver disease that is defined by the presence of inflammation and fibrosis, ultimately leading to cirrhosis and hepatocellular carcinoma. Treatment with human placental extract (HPE) reportedly ameliorates the hepatic injury. We evaluated the effect of HPE treatment in a mouse model of NASH. In the methione- and choline-deficient (MCD) diet-induced liver injury model, fibrosis started from regions adjacent to the sinusoids. We administered the MCD diet with high-salt loading (8% NaCl in the drinking water) to mice deficient in the vasoprotective molecule RAMP2 for 5 weeks, with or without HPE...
September 2017: Heliyon
https://www.readbyqxmd.com/read/29021381/activation-of-fak-and-src-mediates-acquired-sorafenib-resistance-in-a549-human-lung-adenocarcinoma-xenografts
#7
Qingyu S Zhou, Xiaofang Guo, Riya Choksi
Despite encouraging clinical results with sorafenib monotherapy in patients with KRAS-mutant non-small cell lung cancer (NSCLC), the overall survival benefit of this drug is limited by the inevitable development of acquired resistance. The exact mechanism underlying acquired sorafenib resistance in KRAS-mutant NSCLC is unclear. In this study, the mechanism of acquired sorafenib resistance was explored using a biologically relevant xenograft model, which was established by using the A549 human lung adenocarcinoma cell line and an in-vivo derived sorafenib-resistant A549 subline (A549/SRFres)...
October 11, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29020497/stimuli-responsive-nanodiamond-based-biosensor-for-enhanced-metastatic-tumor-site-detection
#8
Xin Wang, Mengjie Gu, Tan Boon Toh, Nurrul Lissa Binti Abdullah, Edward Kai-Hua Chow
Metastasis is often critical to cancer progression and linked to poor survival and drug resistance. Early detection of metastasis, as well as identification of metastatic tumor sites, can improve cancer patient survival. Thus, developing technology to improve the detection of cancer metastasis biomarkers can improve both diagnosis and treatment. In this study, we investigated the use of nanodiamonds to develop a stimuli-responsive metastasis detection complex that utilizes matrix metalloproteinase 9 (MMP9) as a metastasis biomarker, as MMP9 increased expression has been shown to be indicative of metastasis...
October 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28994231/tipe1-suppresses-invasion-and-migration-through-down-regulating-wnt-%C3%AE-catenin-pathway-in-gastric-cancer
#9
Wenwen Liu, Ye Chen, Hua Xie, Yongmin Guo, Dandan Ren, Yupeng Li, Xu Jing, Dongliang Li, Xiao Wang, Miaoqing Zhao, Tianfeng Zhu, Ziying Wang, Xinbing Wei, Fei Gao, Xiaojie Wang, Suxia Liu, Yan Zhang, Fan Yi
Epithelial-mesenchymal transition (EMT) plays an important role in the invasiveness and metastasis of gastric cancer. Therefore, identifying key molecules involved in EMT will provide new therapeutic strategy for treating patients with gastric cancer. TIPE1 is a newly identified member of the TIPE (TNFAIP8) family, and its contributions to progression and metastasis have not been evaluated. In this study, we found that the levels of TIPE1 were significantly reduced and inversely correlated with differentiation status and distant metastasis in primary gastric cancer tissues...
October 10, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28993203/preparation-of-polysomal-fractions-from-mouse-brain-synaptoneurosomes-and-analysis-of-polysomal-bound-mrnas
#10
Bozena Kuzniewska, Magdalena Chojnacka, Jacek Milek, Magdalena Dziembowska
BACKGROUND: Here we describe a detailed, reliable protocol for isolation of polysomal fractions from mouse brain synaptoneurosomes. This method is an important tool to study local protein synthesis in neurons. NEW METHOD: We combined rapid preparation of synaptoneurosomes by filtration with polysome profiling. We provide a detailed protocol highlighting difficulties and critical steps of: i) preparation of synaptoneurosomes; ii) polyribosome fractionation from synaptoneurosomes; iii) extraction of proteins and RNA from sucrose gradient fractions...
October 6, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28992339/stat3-inhibition-attenuates-the-progressive-phenotypes-of-alport-syndrome-mouse-model
#11
Tsubasa Yokota, Kohei Omachi, Mary Ann Suico, Misato Kamura, Haruka Kojima, Ryosuke Fukuda, Keishi Motomura, Keisuke Teramoto, Shota Kaseda, Jun Kuwazuru, Toru Takeo, Naomi Nakagata, Tsuyoshi Shuto, Hirofumi Kai
Background: Alport syndrome (AS) is a hereditary, progressive nephritis caused by mutation of type IV collagen. Previous studies have shown that activation of signal transducer and activator of transcription 3 (STAT3) exacerbates other renal diseases, but whether STAT3 activation exacerbates AS pathology is still unknown. Here we aim to investigate the involvement of STAT3 in the progression of AS. Method: Phosphorylated STAT3 expression was assessed by immunoblotting analysis of kidneys and glomeruli of an AS mouse model ( Col4a5 G5X mutant)...
August 17, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28990072/2-3-5-4%C3%A2-tetrahydroxy-diphenylethylene%C3%A2-2%C3%A2-o%C3%A2-glucoside-inhibits-the-adhesion-and-invasion-of-a549-human-lung-cancer-cells
#12
Ming Xu, Cong Wang, Minglin Zhu, Xianguo Wang, Li Zhang, Jinping Zhao
Lung cancer is considered to be a serious disease that poses a significant threat to human health. 2,3,5,4‑tetrahydroxy diphenylethylene‑2‑O‑glucoside (THSG) is a bioactive compound derived from Polygonum multiflorum Thunb. That has been demonstrated to possess antioxidative, anti‑inflammatory and antitumor activities. However, little is currently known regarding the potential anticancer effects of this compound in lung cancer. Therefore, the present study aimed to investigate the effects of THSG on the adhesion and invasion of A549 human lung cancer cells in vitro, and to identify the putative mechanisms involved...
October 2, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28988968/kiss1-gene-as-a-novel-mediator-of-tgf%C3%AE-mediated-cell-invasion-in-triple-negative-breast-cancer
#13
Jun Tian, Amal A Al-Odaini, Yun Wang, Juliana Korah, Meiou Dai, Lan Xiao, Suhad Ali, Jean-Jacques Lebrun
The invasive and metastatic phenotypes of breast cancer correlate with high recurrence rates and poor survival outcomes. Transforming growth factor-β (TGFβ) promotes tumor progression and metastasis in aggressive breast cancer. Here, we identified the kisspeptin KiSS1 as a downstream target of canonical TGFβ/Smad2 pathway in triple negative breast cancer cells. We also found KiSS1 expression to be required for TGFβ-induced cancer cell invasion. Indeed, knockdown expression of KiSS1 blocked TGFβ-mediated cancer cell invasion as well as metalloproteinase (MMP9) expression and activity...
October 6, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28983590/i%C3%A2-bet151-inhibits-osteoclastogenesis-via-the-rankl-signaling-pathway-in-raw264-7-macrophages
#14
Jing Cheng, Jifu Zheng, Ninghong Guo, Fuming Zi
Excessive bone resorption mediated by osteoclasts may lead to the risk of various lytic bone diseases. In the present study, the effects of I‑BET151, a bromodomain and extra terminal domain protein inhibitor, on osteoclastogenesis in RAW264.7 cells and the underlying mechanism of this process was investigated. Cells were divided into 6 groups, including the control group, receptor activator of nuclear factor‑κB ligand (RANKL) group and 4 other groups containing RANKL and I‑BET151 at different concentrations...
September 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28982031/direct-thrombin-inhibition-with-dabigatran-attenuates-pressure-overload-induced-cardiac-fibrosis-and-dysfunction-in-mice
#15
Anping Dong, Paul Mueller, Fanmuyi Yang, Liping Yang, Andrew Morris, Susan S Smyth
INTRODUCTION: The multifunctional serine protease thrombin exerts proinflammatory and profibrotic cellular effects that may contribute to cardiac remodeling. This study was designed to investigate whether direct thrombin inhibition with dabigatran attenuates myocardial injury in the setting of pressure overload-induced heart failure. MATERIAL AND METHODS: Transverse aortic constriction (TAC) surgery was performed on C57Bl/6J male mice to elicit cardiac hypertrophy...
September 21, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28981980/conditional-reprogramming-of-pediatric-airway-epithelial-cells-a-new-human-model-to-investigate-early-life-respiratory-disorders
#16
Seth Wolf, Geovanny F Perez, Lana Mukharesh, Nataliza Isaza, Diego Preciado, Robert J Freishtat, Dinesh Pillai, Mary C Rose, Gustavo Nino
BACKGROUND: Airway epithelial cells (AEC) are quite difficult to access in newborns and infants. It is critically important to develop robust life-extended models to conduct translational studies in this age group. We propose the use of a recently described cell-culture technology (conditionally reprogrammed cells - CRC) to generate continuous primary cell cultures from nasal and bronchial AEC of young children. METHODS: We collected nasal and/or bronchial AEC from a total of 23 subjects of different ages including newborns/infants/toddlers (0-2 years; N=9), school age children (4-11 years; N=6), and a group of adolescent/adult donors (N=8)...
October 5, 2017: Pediatric Allergy and Immunology
https://www.readbyqxmd.com/read/28981380/astrocytes-promote-progression-of-breast-cancer-metastases-to-the-brain-via-a-kiss1-mediated-autophagy
#17
Natalya Kaverina, Anton V Borovjagin, Zaira Kadagidze, Anatoly Baryshnikov, Maria Baryshnikova, Dmitry Malin, Dhimankrishhna Ghosh, Nameeta Shah, Danny R Welch, Patrik Gabikian, Apollon Karseladze, Charles Cobbs, Ilya V Ulasov
Formation of metastases, also known as cancer dissemination, is an important stage of breast cancer (BrCa) development. KISS1 expression is associated with inhibition of metastases development. Recently we have demonstrated that BrCa metastases to the brain exhibit low levels of KISS1 expression at both mRNA and protein levels. By using multicolor immunofluorescence and coculture techniques here we show that normal adult astrocytes in the brain are capable of promoting metastatic transformation of circulating breast cancer cells localized to the brain through secretion of chemokine CXCL12...
October 5, 2017: Autophagy
https://www.readbyqxmd.com/read/28980922/rs3918242-variant-genotype-frequency-and-increased-timp-2-and-mmp-9-expression-are-positively-correlated-with-cancer-invasion-in-urinary-bladder-cancer
#18
S Pençe, E Özbek, N Ozan Tiryakioğlu, N Ersoy Tunali, H H Pençe, H Tunali
To study the role of MMP9 and TIMP2 genotypes and expression in predisposition to bladder cancer and relation with metastasis. 100 urinary bladder cancer patients and 100 healthy controls were included in the study. rs3918242 and rs8179090 genotypes were determined with PCR-RFLP. Quantitative real-time polymerase chain reaction was employed to assess the MMP-9 and TIMP-2 expression in tumors and adjacent healthy tissues. Variant genotype (TT) for rs3918242 polymorphism and rs8179090 variant genotype are not associated with bladder cancer risk...
September 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28970726/ca-2-calmodulin-dependent-protein-kinase-ii-regulates-colon-cancer-proliferation-and-migration-via-erk1-2-and-p38-pathways
#19
Wei Chen, Ping An, Xiao-Jing Quan, Jun Zhang, Zhong-Yin Zhou, Li-Ping Zou, He-Sheng Luo
AIM: To investigate the role of calmodulin-dependent protein kinase II (CaMKII) in colon cancer growth, migration and invasion. METHODS: CaMKII expression in colon cancer and paracancerous tissues was evaluated via immunochemistry. Transcriptional and posttranscriptional levels of CaMKIIin tissue samples and MMP2, MMP9 and TIMP-1 expression in the human colon cancer cell line HCT116 were assessed by qRT-PCR and western blot. Cell proliferation was detected with the MTT assay...
September 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28969015/depdc1-is-required-for-cell-cycle-progression-and-motility-in-nasopharyngeal-carcinoma
#20
Xuefei Feng, Chundong Zhang, Ling Zhu, Lian Zhang, Hongxia Li, Longxia He, Yan Mi, Yitao Wang, Jiang Zhu, Youquan Bu
DEP domain containing 1 (DEPDC1) is a newly identified cancer-related and cell cycle related gene and has been demonstrated as a novel therapeutic target for bladder cancer. However, the functional involvement and therapeutic potential of DEPDC1 in nasopharyngeal carcinoma (NPC) remains unclear. Our results showed that DEPDC1 was overexpressed at both mRNA and protein levels in NPC tissues compared with normal or non-tumor tissues. The siRNA-mediated DEPDC1 depletion resulted in significant inhibition of proliferation and delay in cell cycle progression in both NPC cell lines, CNE-1 and HNE-1...
September 8, 2017: Oncotarget
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