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https://www.readbyqxmd.com/read/28548550/felis-catus-papillomavirus-type-2-dna-loads-on-kittens-are-transient-and-do-not-reflect-their-susceptibility-to-infection
#1
Neroli A Thomson, David G Thomas, Karin Weidgraaf, John S Munday
Objectives Felis catus papillomavirus type 2 (FcaPV-2) commonly infects the skin of domestic cats, and mounting evidence suggests that the virus could be involved in a subset of feline skin cancers. The reason why some cats develop FcaPV-2-induced disease and others do not is currently unknown. However, it has been shown that kittens in different litters have markedly different FcaPV-2 DNA loads and the aim of this study was to determine whether these differences could be due to inherent differences in susceptibility to infection...
May 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28544240/cell-cycle-synchronization-and-analysis-of-apoptosis-related-gene-in-skin-fibroblasts-from-domestic-cat-felis-silvestris-catus-and-kodkod-leopardus-guigna
#2
D Veraguas, P F Gallegos, F O Castro, L Rodriguez-Alvarez
The kodkod population is in constant decrease and the somatic cell nuclear transfer (SCNT) might help to preserve the genetic pool of this species. The cell cycle synchronization of donor cells plays a crucial role in SCNT. The objective of this research was to evaluate two different methods for quiescence induction, serum starvation (SS) and contact inhibition (CI), both for 1, 3 and 5 days, on skin fibroblast from domestic cat and kodkod. Flow cytometry analysis revealed that in domestic cat, SS and CI, both at 3 and 5 days, increased the percentage of fibroblasts in G0/G1 compared to growing cells (GC) (p < ...
May 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28542523/impact-of-maintenance-immunosuppressive-therapy-on-the-fecal-microbiome-of-renal-transplant-recipients-comparison-between-an-everolimus-and-a-standard-tacrolimus-based-regimen
#3
Gianluigi Zaza, Alessandra Dalla Gassa, Giovanna Felis, Simona Granata, Sandra Torriani, Antonio Lupo
BACKGROUND: The gut microbiome is the full set of microbes living in the gastrointestinal tract and is emerging as an important dynamic/fluid system that, if altered by environmental, dietetic or pharmacological factors, could considerably influence drug response. However, the immunosuppressive drug-induced modifications of this system are still poorly defined. METHODS: We employed an innovative bioinformatics approach to assess differences in the whole-gut microbial metagenomic profile of 20 renal transplant recipients undergoing maintenance treatment with two different immunosuppressive protocols...
2017: PloS One
https://www.readbyqxmd.com/read/28538849/-epidemiological-and-molecular-profile-of-rickettsiosis-in-peruvian-border-locations
#4
Rosa Palacios-Salvatierra, Elizabeth Anaya-Ramírez, Julio Juscamayta-López, Omar Cáceres-Rey, Leonardo Mendoza-Uribe, Patricia Mosquera-Visaloth, Fátima Conceição-Silva
Objectives.: To determine the circulation of Rickettsia in the years 2010 and 2011 in border locations in four regions ofPeru and their clinical epidemiological and molecular characteristics. Materials and Methods.: A cross-sectional study was carried out in Tumbes, Tacna, Madre de Dios, and Loreto. Whole blood samples were obtained from participants for culture and indirect immunofluorescence (IIF) testing. The DNA taken from leukocytes and ectoparasite cultures was used, and those genes detected for Rickettsia that were successfully amplified were sequenced and analyzed...
January 2017: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28525327/in-vitro-fertilizing-potential-of-urethral-and-epididymal-spermatozoa-collected-from-domestic-cats-felis-catus
#5
S Prochowska, W Niżański
The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28521907/chronic-diseases-in-cats-from-the-medieval-site-of-qalh%C3%A3-t-oman
#6
Hervé Monchot, Claude Guintard
Paleopathological information observed in cats (Felis catus, L. 1758) is rarely described in zooarcheological assemblages. This article is intended to present different bone diseases (infections, degenerative and traumatic lesions) affecting several bones (i.e. femur, hip bone, skull, etc.) of at least two cats from the medieval harbor site of Qalhât (Oman) and to inform us about the health status of these felines.
June 2017: International Journal of Paleopathology
https://www.readbyqxmd.com/read/28497745/preventive-efficacy-of-nexgard-spectra-%C3%A2-against-dipylidium-caninum-infection-in-dogs-using-a-natural-flea-ctenocephalides-felis-infestation-model
#7
Frédéric Beugnet, Leon Meyer, Josephus Fourie, Diane Larsen
The efficacy of a monthly oral endectocide product, NexGard Spectra(®) (Merial), a combination of afoxolaner and milbemycin oxime, was evaluated in a flea (Ctenocephalides felis) challenge model for the prevention of Dipylidium caninum tapeworm infection in dogs. The efficacy of treatment with NexGard Spectra(®) was assessed in 10 dogs following weekly flea infestation with metacestode naturally infected fleas and compared with that in 10 untreated control dogs. The 100 fleas deposited weekly on each dog were not removed until Day 35, allowing enough time for their ingestion...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28493189/the-treatment-of-wastewater-containing-pharmaceuticals-in-microcosm-constructed-wetlands-the-occurrence-of-integrons-int1-2-and-associated-resistance-genes-sul1-3-qace%C3%AE-1
#8
Monika Nowrotek, Ewa Kotlarska, Aneta Łuczkiewicz, Ewa Felis, Adam Sochacki, Korneliusz Miksch
The aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1-3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1-2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate and in the effluents were equipped with the sul1-2, int1, and qacEΔ1 resistance determinants, which were introduced into the CW system during inoculation with activated sludge and with the soil attached to the rhizosphere of potted seedlings of Phalaris arundinacea 'Picta' roots (int1)...
May 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28491447/successful-treatment-of-multifocal-pedal-prototheca-wickerhamii-infection-in-a-feline-immunodeficiency-virus-positive-cat-with-multiple-bowenoid-in-situ-carcinomas-containing-papillomaviral-dna-sequences
#9
Allan E Kessell, Derek McNair, John S Munday, Richard Savory, Catriona Halliday, Richard Malik
CASE SUMMARY: A 16-year-old, castrated male, feline immunodeficiency virus (FIV)-positive, domestic shorthair cat developed multiple skin lesions. Most of these were Bowenoid carcinoma in situ and contained DNA sequences consistent with Felis catus papillomavirus type 2. Two additional lesions that developed in the skin and subcutaneous tissues between the digital and carpal pads on the left forelimb and right hindlimb were shown by cytology, histology and culture to be caused by Prototheca wickerhamii...
January 2017: JFMS Open Rep
https://www.readbyqxmd.com/read/28484147/the-presence-of-tick-borne-diseases-in-domestic-dogs-and-cats-living-on-iriomote-jima-and-tsushima-islands
#10
Mao Jikuya, Morihiro Tateno, Masashi Takahashi, Yasuyuki Endo
The Iriomote cat and Tsushima leopard cat are endangered wildcats in Japan and inhabit only Iriomote-jima and Tsushima islands, respectively. Domestic dogs and cats living on Iriomote-jima and Tsushima islands were surveyed to clarify the interrelationship between wildcats and domestic animals regarding tick-borne disease transmission. Pathogen-derived DNA in blood samples was detected by polymerase chain reaction. Babesia gibsoni was detected in dogs of Iriomote-jima, and Hepatozoon felis and hemoplasmas were detected in domestic cats of Tsushima...
May 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28464344/mitochondrial-characteristics-in-oocytes-of-the-domestic-cat-felis-catus-after-in-vitro-maturation-and-vitrification
#11
N Sowińska, K Müller, W Niżański, K Jewgenow
The objective of this study was to evaluate mitochondria in immature and in vitro-matured domestic cat oocytes and to assess for the first time the effect of vitrification on mitochondrial traits. Mitochondrial distribution and aggregation were assessed using confocal microscopy after staining with the fluorescent dye-MitoTracker(®) Red CMXRos. Only cells at the germinal vesicle and the metaphase II stages of nuclear development, representing immature and mature oocytes, respectively, were included in our study...
May 2, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28460208/a-new-animal-model-of-gastric-lymphomagenesis-april-transgenic-mice-infected-by-helicobacter-species
#12
Pauline Floch, Julien Izotte, Julien Guillemaud, Elodie Sifré, Pierre Costet, Benoit Rousseau, Amandine Marine Laur, Alban Giese, Victoria Korolik, Francis Mégraud, Pierre Dubus, Michael Hahne, Philippe Lehours
APRIL is a member of the tumor necrosis factor cytokine family involved in the regulation of B-cell immunity. We present a study of the infection by Helicobacter species of transgenic (Tg) C57BL6 mice, ectopically expressing the human form of APRIL. Wild-type (WT) and APRIL Tg mice were infected with Helicobacter felis and Helicobacter pylori and compared with noninfected animals. Mice were euthanized 18 months after infection, and inflammatory responses and histologic alterations were analyzed. Flow cytometry results revealed that WT-infected mice had less leukocyte infiltration than APRIL Tg-infected mice...
April 28, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28454576/a-field-trial-of-a-fixed-combination-of-permethrin-and-fipronil-effitix-%C3%A2-for-the-treatment-and-prevention-of-flea-infestation-in-dogs-living-with-sheep
#13
Manolis K Chatzis, Dimitris Psemmas, Elias Papadopoulos, Christelle Navarro, Manolis N Saridomichelakis
BACKGROUND: A large number of fleas parasitize dogs living with sheep in Greece. The primary aim of this randomized, blinded, placebo-controlled trial was to examine the efficacy of a permethrin-fipronil combination (Effitix(®)) for the treatment and prevention of flea infestation in dogs living with sheep and the secondary aim was to examine the efficacy of this intervention on flea infestation, pruritus and skin lesions of the people in contact with these dogs. METHODS: Thirty dogs living with sheep and infested by at least 10 fleas and all 80 sheep living on the same premises were randomly allocated into equal groups...
April 28, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28450833/optimal-control-based-stiffness-identification-of-an-ankle-foot-orthosis-using-a-predictive-walking-model
#14
Manish Sreenivasa, Matthew Millard, Martin Felis, Katja Mombaur, Sebastian I Wolf
Predicting the movements, ground reaction forces and neuromuscular activity during gait can be a valuable asset to the clinical rehabilitation community, both to understand pathology, as well as to plan effective intervention. In this work we use an optimal control method to generate predictive simulations of pathological gait in the sagittal plane. We construct a patient-specific model corresponding to a 7-year old child with gait abnormalities and identify the optimal spring characteristics of an ankle-foot orthosis that minimizes muscle effort...
2017: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/28438088/anaplasma-ehrlichia-and-rickettsia-species-infections-in-cats
#15
Maria Grazia Pennisi, Regina Hofmann-Lehmann, Alan D Radford, Séverine Tasker, Sándor Belák, Diane D Addie, Corine Boucraut-Baralon, Herman Egberink, Tadeusz Frymus, Tim Gruffydd-Jones, Katrin Hartmann, Marian C Horzinek, Margaret J Hosie, Albert Lloret, Hans Lutz, Fulvio Marsilio, Etienne Thiry, Uwe Truyen, Karin Möstl
Anaplasma species, Ehrlichia species and Rickettsia species are vector-borne pathogens infecting a wide variety of mammals, but causing disease in very few of them. Infection in cats: Anaplasma phagocytophilum is the most important feline pathogen among these rickettsial organisms, and coinfections are possible. Little information is available on the pathogenesis of these agents in cats. Clinical signs are usually reported soon after tick infestation. They are mostly non-specific, consisting of fever, anorexia and lethargy...
May 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28435002/systemic-arterial-hypertension-secondary-to-chronic-kidney-disease-in-two-captive-born-large-felids
#16
A Leclerc, E Trehiou-Sechi, E M Greunz, C Damoiseaux, J Bouvard, V Chetboul
Systemic arterial hypertension (SHT) has been widely described in the domestic cat (Felis catus). In these feline patients, SHT is considered as the most common vascular disorder of middle-aged to older animals, and secondary SHT related to chronic kidney disease (CKD) represents the most common form of the disease. We describe here the first two cases of spontaneous SHT in large felids, i.e. one 18-year old, 34.4 kg, male North-Chinese leopard (Panthera pardus japonensis, case #1) and one 20-year old, 28...
April 20, 2017: Journal of Veterinary Cardiology: the Official Journal of the European Society of Veterinary Cardiology
https://www.readbyqxmd.com/read/28433729/comparative-vertical-transmission-of-rickettsia-by-dermacentor-variabilis-and-amblyomma-maculatum
#17
Emma K Harris, Victoria I Verhoeve, Kaikhushroo H Banajee, Jacqueline A Macaluso, Abdu F Azad, Kevin R Macaluso
The geographical overlap of multiple Rickettsia and tick species coincides with the molecular detection of a variety of rickettsial agents in what may be novel tick hosts. However, little is known concerning transmissibility of rickettsial species by various tick hosts. To examine the vertical transmission potential between select tick and rickettsial species, two sympatric species of ticks, Dermacentor variabilis and Amblyomma maculatum, were exposed to five different rickettsial species, including Rickettsia rickettsii, Rickettsia parkeri, Rickettsia montanensis, Rickettsia amblyommatis, or flea-borne Rickettsia felis...
April 6, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28427962/first-report-of-a-rickettsia-asembonensis-related-infecting-fleas-in-brazil
#18
Arannadia Barbosa Silva, Vinicius Figueiredo Vizzoni, Andréa Pereira Costa, Francisco Borges Costa, Jonas Moraes-Filho, Marcelo Bahia Labruna, Gilberto Salles Gazêta, Rita de Maria Seabra Nogueira
The present study was performed in a non-endemic area for spotted fever (SF) in Imperatriz microregion, state of Maranhão, Brazil. Blood samples and ectoparasites were collected from 300 dogs of the Imperatriz microregion. Canine serum samples were tested individually by indirect immunofluorescence assay (IFA), using five Rickettsia isolates from Brazil. Antibodies reactive to at least one of the five species of Rickettsia were detected in 1.6% of the dogs (5/300). These sera were considered reactive to Rickettsia rickettsii and Rickettsia amblyommatis or very closely related species...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28426412/fatal-aelurostrongylosis-in-a-kitten-in-serbia
#19
Pavle Gavrilović, Milijan Jovanović, Aleksandra Gavrilović, Slađan Nešić
This paper describes a case of aelurostrongylosis in a four-month old female domestic cat (Felis catus) from South Banat, Serbia. The kitten that had died suddenly without signs of illness was autopsied in the Veterinary Specialised Institute "Pančevo". The macroscopic finding was typical of granulomatous pneumonia and subsequently, histopathology revealed verminous pneumonia. Based on the parasite morphology, it was confirmed that the lung lesions were caused by Aelurostrongylus abstrusus. The present finding contributes to the knowledge of the prevalence of this underestimated parasite in the Balkan Peninsula...
June 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28424664/spotted-fever-epidemiology-and-vector-rickettsia-host-relationship-in-rio-de-janeiro-state
#20
Diego C Montenegro, Karla Bitencourth, Stefan V de Oliveira, Ana P Borsoi, Karen M Cardoso, Maria S B Sousa, Cristina Giordano-Dias, Marinete Amorim, Nicolau M Serra-Freire, Gilberto S Gazêta, Reginaldo P Brazil
The eco-epidemiological scenario of spotted fever (SF), a tick-borne disease that affects humans and other animals in several countries around the world, was analyzed in Rio de Janeiro (RJ) State, Brazil. During the last 34 years, 990 SF cases were reported in RJ (the Brazilian state with the highest population density), including 116 cases confirmed by serology (RIFI) or PCR, among 42.39% of the municipalities with reported cases of SF. The epidemiologic dynamics of SF in RJ State are very heterogeneous in time and space, with outbreaks, high mortality rates and periods of epidemiological silence (no SF cases reported)...
2017: Frontiers in Microbiology
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