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Endocytosis

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https://www.readbyqxmd.com/read/29691301/plant-endomembrane-dynamics-studies-of-k-h-antiporters-provide-insights-on-the-effects-of-ph-and-ion-homeostasis
#1
Heven Sze, Salil Chanroj
Plants remodel their cells through the dynamic endomembrane system. Intracellular pH is important for membrane trafficking, but the determinants of pH homeostasis are poorly defined in plants. Electrogenic proton (H+) pumps depend on counter-ion fluxes to establish transmembrane pH gradients at the plasma membrane and endomembranes. Vacuolar-type H+-ATPase-mediated acidification of the trans-Golgi network (TGN) is crucial for secretion and membrane recycling. Pump and counter-ion fluxes are unlikely to fine-tune pH; rather, alkali cation/H+ antiporters, which can alter pH and/or cation homeostasis locally and transiently, are prime candidates...
April 24, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29691252/high-usp6nl-levels-in-breast-cancer-sustain-chronic-akt-phosphorylation-and-glut1-stability-fueling-aerobic-glycolysis
#2
Daniele Avanzato, Emanuela Pupo, Nadia Ducano, Claudio Isella, Giovanni Bertalot, Chiara Luise, Salvatore Pece, Alejandra Bruna, Oscar M Rueda, Carlos Caldas, Pier Paolo Di Fiore, Anna Sapino, Letizia Lanzetti
USP6NL, also named RN-tre, is a GTPase activating protein (GAP) involved in control of endocytosis and signal transduction. Here we report that USP6NL is overexpressed in breast cancer (BC), mainly of the basal-like/integrative cluster 10 subtype. Increased USP6NL levels were accompanied by gene amplification and were associated with worse prognosis in the METABRIC dataset, retaining prognostic value in multivariable analysis. High levels of USP6NL in BC cells delayed endocytosis and degradation of the epidermal growth factor receptor (EGFR), causing chronic AKT activation...
April 24, 2018: Cancer Research
https://www.readbyqxmd.com/read/29689431/calcium-deprivation-enhances-non-selective-fluid-phase-endocytosis-and-modifies-membrane-lipid-profiles-in-arabidopsis-roots
#3
Ting Zhang, Ju Yang, Yongwei Sun, Yan Kang, Jia Yang, Zhi Qi
Ecological studies have revealed significant decreases in calcium (Ca) levels in various soils, and widely occurring physiological Ca deficits worldwide. These changes may cause decreases in plant diversity and increases in plant vulnerability to environmental stress, but the underlying cellular mechanism is not well understood. In this study, we found that in Arabidopsis thaliana roots, deprivation of Ca2+ , but not other minerals, dramatically enhanced plasma membrane invagination, endosome formation, and trafficking to the vacuole through the trans-Golgi network and pre-vacuole compartment, a typical pathway of endocytosis...
April 10, 2018: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29689425/the-lysosome-system-is-severely-impaired-in-a-cellular-model-of-neurodegeneration-induced-by-hsv-1-and-oxidative-stress
#4
Henrike Kristen, Isabel Sastre, Teresa Muñoz-Galdeano, Maria Recuero, Jesus Aldudo, Maria J Bullido
The causal agent(s) and molecular mechanisms of Alzheimer's disease (AD) remain unclear. Mounting evidence suggests that herpes simplex virus type 1 (HSV-1) infection is involved in the AD pathogenesis. Oxidative stress (OS) may also be crucial in the AD development. Our group previously reported that both HSV-1 and OS trigger the appearance of AD-type neurodegeneration markers. The main aim of the present study was to identify the mechanisms involved in this triggering. Expression studies revealed the involvement of a set of OS-regulated genes in HSV-1-infected cells and in cells harboring the Swedish mutation of the amyloid beta precursor protein gene...
March 29, 2018: Neurobiology of Aging
https://www.readbyqxmd.com/read/29688802/infliximab-restores-colonic-barrier-to-adherent-invasive-e-coli-in-crohn-s-disease-via-effects-on-epithelial-lipid-rafts
#5
Olena Yakymenko, Ida Schoultz, Elisabeth Gullberg, Magnus Ström, Sven Almer, Conny Wallon, Arthur Wang, Åsa V Keita, Barry J Campbell, Derek M McKay, Johan D Söderholm
OBJECTIVE: Infliximab is important in the therapeutic arsenal of Crohn's disease (CD). However, its effect on mucosal barrier function is not fully understood. Adherent-invasive Escherichia coli (AIEC) are important in CD pathophysiology, but the transmucosal uptake routes are partly unknown. We investigated effects of infliximab on uptake of colon-specific AIEC HM427 across CD colonic mucosa. MATERIALS AND METHODS: Endoscopic biopsies from non-inflamed colon of seven patients with CD, before and after two infliximab infusions, and eight non-inflammation controls, were mounted in Ussing chambers...
April 24, 2018: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/29687130/size-dependent-endocytosis-and-a-dynamic-release-model-of-nanoparticles
#6
Ruihao Pan, Guoqiang Liu, Yongsan Li, Yen Wei, Shurun Li, Lei Tao
Polymeric nanoparticles for drug delivery are attracting broad interest along with the rapid development of biomedical and healthcare research. Here, we prepared a series of nanocapsules via electrostatic precipitation of chitosan and lecithin micelles. These nanocapsules have controlled diameters (∼25-200 nm) that only slightly changed after several lyophilization-dissolving cycles, suggesting their excellent stability for long-term storage. In cell experiments, these nanocapsules obviously reduced the cytotoxicity of encapsulated small molecules, and clearly showed size-dependent endocytosis...
April 24, 2018: Nanoscale
https://www.readbyqxmd.com/read/29686427/a-mobile-endocytic-network-connects-clathrin-independent-receptor-endocytosis-to-recycling-and-promotes-t-cell-activation
#7
Ewoud B Compeer, Felix Kraus, Manuela Ecker, Gregory Redpath, Mayan Amiezer, Nils Rother, Philip R Nicovich, Natasha Kapoor-Kaushik, Qiji Deng, Guerric P B Samson, Zhengmin Yang, Jieqiong Lou, Michael Carnell, Haig Vartoukian, Katharina Gaus, Jérémie Rossy
Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that control the organization of recycling endosomes and how they connect to receptor endocytosis. Here, we follow the endocytic journey of the T cell receptor (TCR), from internalization at the plasma membrane to recycling back to the immunological synapse...
April 23, 2018: Nature Communications
https://www.readbyqxmd.com/read/29686402/targeting-glp-1-receptor-trafficking-to-improve-agonist-efficacy
#8
Ben Jones, Teresa Buenaventura, Nisha Kanda, Pauline Chabosseau, Bryn M Owen, Rebecca Scott, Robert Goldin, Napat Angkathunyakul, Ivan R Corrêa, Domenico Bosco, Paul R Johnson, Lorenzo Piemonti, Piero Marchetti, A M James Shapiro, Blake J Cochran, Aylin C Hanyaloglu, Asuka Inoue, Tricia Tan, Guy A Rutter, Alejandra Tomas, Stephen R Bloom
Glucagon-like peptide-1 receptor (GLP-1R) activation promotes insulin secretion from pancreatic beta cells, causes weight loss, and is an important pharmacological target in type 2 diabetes (T2D). Like other G protein-coupled receptors, the GLP-1R undergoes agonist-mediated endocytosis, but the functional and therapeutic consequences of modulating GLP-1R endocytic trafficking have not been clearly defined. Here, we investigate a series of biased GLP-1R agonists with variable propensities for GLP-1R internalization and recycling...
April 23, 2018: Nature Communications
https://www.readbyqxmd.com/read/29684527/co-delivery-of-docetaxel-and-gemcitabine-using-pegylated-self-assembled-stealth-nanoparticles-for-improved-breast-cancer-therapy
#9
Varun Kushwah, Sameer S Katiyar, Ashish Kumar Agrawal, Ramesh C Gupta, Sanyog Jain
The present report deals with conjugation of dual drug; docetaxel (DTX) and gemcitabine (GEM) with linker poly-ethylene-glycol (PEG) to develop amphiphilic molecule having self-assembled property. The synthesized conjugate (DTX-PEG-GEM) demonstrated critical micelle concentration (CMC) in the range of 5-10μg/ml which self-assembled to form NPs with size 124.2±5.7. Remarkably higher coumarin-6 (C-6) fluorescence signals observed in case of C-6 loaded NPs, suggested enhanced cellular uptake via clathrin mediated endocytosis...
April 20, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29683423/optical-detection-of-three-modes-of-endocytosis-at-hippocampal-synapses
#10
Natali L Chanaday, Ege T Kavalali
Coupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, using rat hippocampal neurons, we optically monitored single synaptic vesicle endocytosis with high time resolution using the vesicular glutamate transporter, synaptophysin and the V0a1 subunit of the vacuolar ATPase as probes...
April 23, 2018: ELife
https://www.readbyqxmd.com/read/29681477/a-forward-genetic-screen-in-zebrafish-identifies-the-g-protein-coupled-receptor-casr-as-a-modulator-of-sensorimotor-decision-making
#11
Roshan A Jain, Marc A Wolman, Kurt C Marsden, Jessica C Nelson, Hannah Shoenhard, Fabio A Echeverry, Christina Szi, Hannah Bell, Julianne Skinner, Emilia N Cobbs, Keisuke Sawada, Amy D Zamora, Alberto E Pereda, Michael Granato
Animals continuously integrate sensory information and select contextually appropriate responses. Here, we show that zebrafish larvae select a behavioral response to acoustic stimuli from a pre-existing choice repertoire in a context-dependent manner. We demonstrate that this sensorimotor choice is modulated by stimulus quality and history, as well as by neuromodulatory systems-all hallmarks of more complex decision making. Moreover, from a genetic screen coupled with whole-genome sequencing, we identified eight mutants with deficits in this sensorimotor choice, including mutants of the vertebrate-specific G-protein-coupled extracellular calcium-sensing receptor (CaSR), whose function in the nervous system is not well understood...
April 6, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29680490/14-3-3%C3%AE-%C3%AE-a-interacts-with-glycoprotein-of-spring-viremia-of-carp-virus-and-positively-affects-viral-entry
#12
Buxin Chen, Chen Li, Yeda Wang, Yuanan Lu, Fang Wang, Xueqin Liu
Spring viremia of carp virus (SVCV) is a fierce pathogen causing high mortality in the common carp. The glycoprotein (G protein) of SVCV is a pivotal component of the viral structure, located in the surface of the virion, and plays a key role in viral endocytosis. In this study, tandem affinity purification (TAP) followed by mass spectrometry analysis (LC-MS/MS) was carried out to search for novel host molecules that interact with SVCV G protein and a 14-3-3β/α-A protein was identified. The level of 14-3-3β/α-A mRNA expression was dramatically down regulated by SVCV infection...
April 19, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29679667/systemic-delivery-of-bace1-sirna-through-neuron-targeted-nanocomplexes-for-treatment-of-alzheimer-s-disease
#13
Pengzhen Wang, Xiaoyao Zheng, Qian Guo, Peng Yang, Xiaoying Pang, Kang Qian, Wei Lu, Qizhi Zhang, Xinguo Jiang
β-site amyloid precursor protein cleaving enzyme 1 (BACE1) is a key enzyme to cleave the amyloid precursor protein to develop Alzheimer's disease (AD). Reducing BACE1 expression in central neuron through RNA interference technology shows great promise to overcome AD. However, to obtain an efficient and neurons-specific delivery of siRNA against BACE1 through systemic administration remains challenging. Here, we design and prepare siRNA nano-carriers based on PEGylated poly(2-(N,N-dimethylamino) ethyl methacrylate) (PEG-PDMAEMA) modified with both the CGN peptide for blood-brain barrier (BBB) penetration and the Tet1 peptide for neuron-specific binding...
April 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29678956/local-actin-polymerization-during-endocytic-carrier-formation
#14
REVIEW
Claudia Hinze, Emmanuel Boucrot
Extracellular macromolecules, pathogens and cell surface proteins rely on endocytosis to enter cells. Key steps of endocytic carrier formation are cargo molecule selection, plasma membrane folding and detachment from the cell surface. While dedicated proteins mediate each step, the actin cytoskeleton contributes to all. However, its role can be indirect to the actual molecular events driving endocytosis. Here, we review our understanding of the molecular steps mediating local actin polymerization during the formation of endocytic carriers...
April 20, 2018: Biochemical Society Transactions
https://www.readbyqxmd.com/read/29677448/self-assembled-ph-responsive-polymeric-micelles-for-highly-efficient-non-cytotoxic-delivery-of-doxorubicin-chemotherapy-to-inhibit-macrophage-activation-in-vitro-investigation
#15
Zhi-Sheng Liao, Shan-You Huang, Jyun-Jie Huang, Jem-Kun Chen, Ai-Wei Lee, Juin-Yih Lai, Duu-Jong Lee, Chih-Chia Cheng
Self-assembled pH-responsive polymeric micelles, a combination of hydrophilic poly(ethylene glycol) segments and hydrogen bonding interactions within a biocompatible polyurethane substrate, can spontaneously self-assemble into highly controlled, nanosized micelles in aqueous solution. These newly-developed micelles exhibit excellent pH-responsive behavior and biocompatibility, highly controlled drug (doxorubicin; DOX) release behavior and high drug encapsulation stability in different aqueous environments, making the micelles highly attractive potential candidates for safer, more effective drug delivery in applications such as cancer chemotherapy...
April 20, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29675232/diselenolane-mediated-cellular-uptake
#16
Nicolas Chuard, Amalia I Poblador-Bahamonde, Lili Zong, Eline Bartolami, Jana Hildebrandt, Wolfgang Weigand, Naomi Sakai, Stefan Matile
The emerging power of thiol-mediated uptake with strained disulfides called for a move from sulfur to selenium. We report that according to results with fluorescent model substrates, cellular uptake with 1,2-diselenolanes exceeds uptake with 1,2-dithiolanes and epidithiodiketopiperazines with regard to efficiency as well as intracellular localization. The diselenide analog of lipoic acid performs best. This 1,2-diselenolane delivers fluorophores efficiently to the cytosol of HeLa Kyoto cells, without detectable endosomal capture as with 1,2-dithiolanes or dominant escape into the nucleus as with epidithiodiketopiperazines...
February 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29674564/observing-the-cell-in-its-native-state-imaging-subcellular-dynamics-in-multicellular-organisms
#17
Tsung-Li Liu, Srigokul Upadhyayula, Daniel E Milkie, Ved Singh, Kai Wang, Ian A Swinburne, Kishore R Mosaliganti, Zach M Collins, Tom W Hiscock, Jamien Shea, Abraham Q Kohrman, Taylor N Medwig, Daphne Dambournet, Ryan Forster, Brian Cunniff, Yuan Ruan, Hanako Yashiro, Steffen Scholpp, Elliot M Meyerowitz, Dirk Hockemeyer, David G Drubin, Benjamin L Martin, David Q Matus, Minoru Koyama, Sean G Megason, Tom Kirchhausen, Eric Betzig
True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo...
April 20, 2018: Science
https://www.readbyqxmd.com/read/29674246/a-microrna-expression-signature-of-the-postprandial-state-in-response-to-a-high-saturated-fat-challenge
#18
Sergio Lopez, Beatriz Bermudez, Sergio Montserrat-de la Paz, Rocio Abia, Francisco J G Muriana
The postprandial hypertriglyceridemia is an important and largely silent disturbance involved in the genesis of numerous pathological conditions. Exaggerated and prolonged states of postprandial hypertriglyceridemia are frequently related to the ingestion of meals enriched in saturated fatty acids (SFAs). MicroRNAs are noncoding RNAs that function as gene regulators and play significant roles in both health and disease. However, differential miRNA expression between fasting and postprandial states has never been elucidated...
March 18, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29673839/development-of-a-macrophage-targeting-and-phagocytosis-inducing-bio-nanocapsule-based-nanocarrier-for-drug-delivery
#19
Hao Li, Kenji Tatematsu, Masaharu Somiya, Masumi Iijima, Shun' Ichi Kuroda
Macrophage hyperfunction or dysfunction is tightly associated with various diseases, such as osteoporosis, inflammatory disorder, and cancers. However, nearly all conventional drug delivery system (DDS) nanocarriers utilize endocytosis for entering target cells; thus, the development of macrophage-targeting and phagocytosis-inducing DDS nanocarriers for treating these diseases is required. In this study, we developed a hepatitis B virus (HBV) envelope L particle (i.e., bio-nanocapsule (BNC)) outwardly displaying a tandem form of protein G-derived IgG Fc-binding domain and protein L-derived IgG Fab-binding domain (GL-BNC)...
April 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29673366/improving-cytocompatibility-of-cdte-quantum-dots-by-schiff-base-coordinated-lanthanides-surface-doping
#20
Hana Buchtelova, Vladislav Strmiska, Zuzana Skubalova, Simona Dostalova, Petr Michalek, Sona Krizkova, David Hynek, Lukas Kalina, Lukas Richtera, Amitava Moulick, Vojtech Adam, Zbynek Heger
BACKGROUND: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable toxicity. This hinders QDs applicability in living systems. Surface chemistry has a profound impact on biological behavior of QDs. This study describes a two-step synthesis of QDs formed by CdTe core doped with Schiff base ligand for lanthanides [Ln (Yb3+ , Tb3+ and Gd3+ )] as novel cytocompatible fluorophores...
April 19, 2018: Journal of Nanobiotechnology
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