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Endocytosis

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https://www.readbyqxmd.com/read/28527044/endocytic-vesicle-rupture-is-a-conserved-mechanism-of-cellular-invasion-by-amyloid-proteins
#1
William P Flavin, Luc Bousset, Zachary C Green, Yaping Chu, Stratos Skarpathiotis, Michael J Chaney, Jeffrey H Kordower, Ronald Melki, Edward M Campbell
Numerous pathological amyloid proteins spread from cell to cell during neurodegenerative disease, facilitating the propagation of cellular pathology and disease progression. Understanding the mechanism by which disease-associated amyloid protein assemblies enter target cells and induce cellular dysfunction is, therefore, key to understanding the progressive nature of such neurodegenerative diseases. In this study, we utilized an imaging-based assay to monitor the ability of disease-associated amyloid assemblies to rupture intracellular vesicles following endocytosis...
May 19, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28526602/palmitoyl-ascorbate-and-doxorubicin-co-encapsulated-liposome-for-synergistic-anticancer-therapy
#2
Yue Yang, Xiaoyu Lu, Qi Liu, Yu Dai, Xiaojie Zhu, Yanli Wen, Jiaqiu Xu, Yang Lu, Di Zhao, Xijing Chen, Ning Li
Combination therapy with two drugs and nanoparticle-based drug delivery systems are widely applied to reduce the adverse effects of traditional treatment by chemotherapeutic drugs. Palmitoyl ascorbate (PA) as a lipophilic derivative of ascorbic acid shows the advantages in cancer treatment. The aim of the study was to prepare a doxorubicin (DOX) and PA co-loaded liposome to synergistically treat tumor and effectively alleviate the toxicity caused by DOX. The effects were evaluated by in vitro and in vivo studies...
May 16, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28525956/aggregation-induced-emission-fluorogens-light-cells-via-microtubules-accessing-the-mechanisms-of-intracellular-trafficking-of-ionic-substances
#3
Shixin Zhou, Yijun Xia, Yinan Liu, Qihua He, Bo Song
Understanding the enrichment and intracellular trafficking of substances is centrally important to the biological systems. Here, employing an amphiphilic molecule (denoted by TPE-11) bearing tetraphenylethene moiety, known for aggregation induced emission property, we demonstrated its localization shifting in Hela cells after prolonged incubation. Through a set of delicately designed experiments, we found that one type of cytoskeleton, i.e. microtubule, is responsible for the intracellular transportation regardless of the sources of fluorogens, via endocytosis pathways or not...
May 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28525949/capsaicin-loaded-folic-acid-conjugated-lipid-nanoparticles-for-enhanced-therapeutic-efficacy-in-ovarian-cancers
#4
Lin Lv, Yu-Xin Zhuang, Hui-Wu Zhang, Nan-Nan Tian, Wen-Zhen Dang, Shao-Yu Wu
In this study, folic acid-conjugated lipid nanoparticles were successfully prepared to enhance the active targeting of capsaicin (CAP) in ovarian cancers. The particles were nanosized and exhibited a controlled release of drug in the physiological conditions. The folic acid (FA)-conjugated system exhibited a remarkably higher uptake of nanoparticles in the cancer cells compared to that of non-targeted system. The folate-conjugated CAP-loaded lipid nanoparticles (CFLN) upon interacting with cancer cells were internalized via receptor-mediated endocytosis mechanism and resulted in higher concentration in the cancer cells...
May 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28521780/ultrasound-and-microbubble-induced-release-from-intracellular-compartments
#5
Farah Hussein, Costin Antonescu, Raffi Karshafian
BACKGROUND: Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis...
May 18, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28521291/ube2j2-promotes-hepatocellular-carcinoma-cell-epithelial-mesenchymal-transition-and-invasion-in-vitro
#6
Shaopeng Chen, Ying Tan, Haihua Deng, Zhifa Shen, Yanhong Liu, Pan Wu, Chunyan Tan, Yuyang Jiang
Ubiquitin-conjugating enzyme E2 J2 (UBE2J2) is an ubiquitin proteasome component that responds to proteotoxic stress. We found that UBE2J2 was highly expressed in cellular protrusions of HCCLM3 metastatic hepatocellular carcinoma (HC) cells. Immunohistochemical analyses showed that UBE2J2 was expressed at higher levels in HC patient tissues than in corresponding non-tumor tissues. Because cellular protrusions are important for cell invasion, we hypothesized that UBE2J2 promotes HC cell invasion. We used chip-based surface plasmon resonance (SPR) to assess possible mechanisms of UBE2J2-regulated HCCLM3 cell invasion...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28521013/flagellin-peptide-flg22-gains-access-to-long-distance-trafficking-in-arabidopsis-via-its-receptor-fls2
#7
Joanna Jelenska, Sandra M Davern, Robert F Standaert, Saed Mirzadeh, Jean T Greenberg
Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells and whether they are transported to distal tissues. We used biologically active fluorophore and radiolabeled peptides to establish that flg22 moves to distal organs with the closest vascular connections. Remarkably, entry into the plant cell via endocytosis together with the FLS2 receptor is needed for delivery to vascular tissue and long-distance transport of flg22...
March 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28520088/clathrin-mediated-endocytosis-is-required-for-ane-30-100k-induced-autophagy
#8
Mei-Huei Lin, Young-Chau Liu, Shyun-Yeu Liu, Fang-Chi Chen, Pei-Jung Yang, Guan-Hua Li, Pang-Yen Liu, Ching-Yu Yen
BACKGROUND: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. METHODS: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells...
May 18, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/28516118/quantitative-measurements-of-hiv-1-and-dextran-capture-by-human-monocyte-derived-dendritic-cells-mddcs
#9
Mickaël M Ménager, Dan R Littman
The aim of this protocol is to describe how to measure and quantify the amount of HIV-1 particles and dextran molecules internalized in human monocyte derived dendritic cells (MDDCs), using three different techniques: flow cytometry, quantitative PCR and confocal microscopy. BACKGROUND: This protocol was developed in order to assess the changes of HIV-1 internalization upon disruption of actin nucleation in human monocyte derived dendritic cells. Following a shRNA screen to identify genes important for HIV-1 transfer from dendritic cells to T cells, we observed that a disruption of actin nucleation leads to a switch from actin rich dendrites to blebs, due to an excess of actomyosin contraction...
November 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28515429/endocytic-uptake-of-monomeric-amyloid-%C3%AE-peptides-is-clathrin-and-dynamin-independent-and-results-in-selective-accumulation-of-a%C3%AE-1-42-compared-to-a%C3%AE-1-40
#10
Emelie Wesén, Gavin D M Jeffries, Maria Matson Dzebo, Elin K Esbjörner
Intraneuronal accumulation of amyloid-β (Aβ) peptides represent an early pathological feature in Alzheimer's disease. We have therefore utilized flow cytometry and confocal microscopy in combination with endocytosis inhibition to explore the internalisation efficiency and uptake mechanisms of Aβ(1-40) and Aβ(1-42) monomers in cultured SH-SY5Y cells. We find that both variants are constitutively internalised via endocytosis and that their uptake is proportional to cellular endocytic rate. Moreover, SH-SY5Y cells internalise consistently twice the amount of Aβ(1-42) compared to Aβ(1-40); an imaging-based quantification showed that cells treated with 1 µM peptide for 8 h contained 800,000 peptides of Aβ(1-42) and 400,000 of Aβ(1-40)...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28515088/loss-of-nherf-1-expression-prevents-dopamine-mediated-na-k-atpase-regulation-in-renal-proximal-tubule-cells-from-rat-models-of-hypertension-aged-f344-and-shrs
#11
Michelle T Barati, Corey J Ketchem, Michael L Merchant, Walter B Kusiak, Pedro A Jose, Edward J Weinman, Amanda J LeBlanc, Eleanor D Lederer, Syed J Khundmiri
Dopamine decreases Na-K ATPase (NKA) activity by PKC-dependent phosphorylation and endocytosis of the NKA alpha1. Dopamine-mediated regulation of NKA is impaired in aging and some forms of hypertension. Using opossum (OK) proximal tubule cells (PTCs), we demonstrated that sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) associates with NKA alpha1 and dopamine-1 receptor (D1R). This association is required for the dopamine-mediated regulation of NKA. In OK cells dopamine decreases NHERF-1 association with NKA alpha1 but increases its association with D1R...
May 17, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28513921/chitin-induced-and-chitin-elicitor-receptor-kinase1-cerk1-phosphorylation-dependent-endocytosis-of-arabidopsis-thaliana-lysin-motif-containing-receptor-like-kinase5-lyk5
#12
Jan Erwig, Hassan Ghareeb, Michaela Kopischke, Ronja Hacke, Alexandra Matei, Elena Petutschnig, Volker Lipka
To detect potential pathogens, plants perceive the fungal polysaccharide chitin through receptor complexes containing lysin motif receptor-like kinases (LysM-RLKs). To investigate the ligand-induced spatial dynamics of chitin receptor components, we studied the subcellular behaviour of two Arabidopsis thaliana LysM-RLKs involved in chitin signalling, CHITIN ELICITOR RECEPTOR KINASE1 (CERK1) and LYSIN MOTIF-CONTAINING RECEPTOR-LIKE KINASE5. We performed standard and quantitative confocal laser scanning microscopy on stably transformed A...
May 17, 2017: New Phytologist
https://www.readbyqxmd.com/read/28512151/papers-of-note-in-science356-6338
#13
Annalisa M VanHook
This week's articles demonstrate how T cells search for ligands; a role for the endoplasmic reticulum in the nonclathrin-mediated endocytosis of a growth factor receptor; and growth coupling between distant bacterial colonies that enables the cells to share limited resources.
May 16, 2017: Science Signaling
https://www.readbyqxmd.com/read/28511876/chondroitin-sulfate-functionalized-polyamidoamine-as-a-tumor-targeted-carrier-for-mir-34a-delivery
#14
Wenqi Chen, Yong Liu, Xiao Liang, Yu Huang, Quanshun Li
Chondroitin sulfate (CS) was modified on a polyamidoamine dendrimer (PAMAM) through Michael addition to construct a tumor-targeted carrier CS-PAMAM for miR-34a delivery. The derivative CS-PAMAM was demonstrated to achieve an efficient cellular uptake of miR-34a in a CD44-dependent endocytosis way and further facilitate the endosomal escape of miR-34a after 4 h. Through the miR-34a delivery, obvious inhibition of cell proliferation could be detected which was attributed to the enhancement of cell apoptosis and cell cycle arrest, and meanwhile the cell migration and invasion has been observed to be inhibited...
May 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28508624/temperature-treatment-of-highly-porous-zirconium-containing-metal-organic-frameworks-extends-drug-delivery-release
#15
Michelle H Teplensky, Marcus Fantham, Peng Li, Timothy C Wang, Joshua P Mehta, Laurence J Young, Peyman Z Moghadam, Joseph T Hupp, Omar K Farha, Clemens F Kaminski, David Fairen-Jimenez
Utilizing metal-organic frameworks (MOFs) as a biological carrier can lower the amount of active pharmaceutical ingredient (API) required in cancer treatments to provide a more efficacious therapy. In this work, we have developed a temperature treatment process for delaying the release of a model drug compound from the pores of NU-1000 and NU-901, while taking care to utilize these MOFs' large pore volume and size to achieve exceptional model drug loading percentages over 35 wt.%. Video-rate super-resolution microscopy reveals movement of MOF particles when located outside of the cell boundary, and their subsequent immobilization when taken up by the cell...
May 16, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28508421/subversion-of-host-kinases-a-key-network-in-cellular-signaling-hijacked-by-helicobacter-pylori-caga
#16
REVIEW
Nicole Tegtmeyer, Matthias Neddermann, Carmen Isabell Asche, Steffen Backert
Helicobacter pylori is a paradigm of persistent pathogens and major risk factor for developing severe diseases including adenocarcinoma in the human stomach. An important bacterial factor linked to gastric disease progression is the cag pathogenicity island-encoded type-IV secretion system (T4SS) effector protein CagA. Translocated CagA undergoes tyrosine phosphorylation at EPIYA-motifs and then activates or inactivates multiple host signaling proteins in a phosphorylation-dependent and phosphorylation-independent fashion...
May 15, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28507533/cytokine-receptor-endocytosis-new-kinase-activity-dependent-and-independent-roles-of-pi3k
#17
REVIEW
Ping-Hung Chen, Huiyu Yao, Lily Jun-Shen Huang
Type I and II cytokine receptors are cell surface sensors that bind cytokines in the extracellular environment and initiate intracellular signaling to control processes such as hematopoiesis, immune function, and cellular growth and development. One key mechanism that regulates signaling from cytokine receptors is through receptor endocytosis. In this mini-review, we describe recent advances in endocytic regulations of cytokine receptors, focusing on new paradigms by which PI3K controls receptor endocytosis through both kinase activity-dependent and -independent mechanisms...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28507272/phosphatidylinositol-5-phosphate-4-kinase-regulates-early-endosomal-dynamics-during-clathrin-mediated-endocytosis
#18
Kumari Kamalesh, Deepti Trivedi, Sarah Toscano, Sanjeev Sharma, Sourav Kolay, Padinjat Raghu
Endocytic turnover is essential to regulate the protein composition and function of the plasma membrane thus regulating the plasma membrane levels of many receptors. In Drosophila photoreceptors, photon absorption by the GPCR rhodopsin 1 (Rh1) triggers its endocytosis by clathrin-mediated endocytosis (CME). We find that CME of Rh1 is regulated by phosphatidylinositol 5 phosphate 4-kinase (PIP4K). Flies lacking PIP4K show mislocalization of Rh1 on expanded endomembranes within the cell body. This mislocalization of Rh1 was dependent on the formation of an expanded Rab5 compartment...
May 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28504350/the-arfgap-protein-moglo3-regulates-the-development-and-pathogenicity-of-magnaporthe-oryzae
#19
Shengpei Zhang, Xiu Liu, Lianwei Li, Rui Yu, Jialiang He, Haifeng Zhang, Xiaobo Zheng, Ping Wang, Zhengguang Zhang
The ADP ribosylation factor (Arf) and the coat protein complex I (COPI) are both involved in vesicle transport. Together with GTPase-activating proteins (ArfGAPs) and guanine exchange factors (ArfGEFs) that regulate the activity of Arf, they govern vesicle formation, COPI trafficking, and the maintenance of the Golgi complex. In an ongoing effort to study the role of membrane trafficking in pathogenesis of the rice blast fungus Magnaporthe oryzae, we identified MoGlo3 as an ArfGAP protein that is homologous to Glo3p of the budding yeast Saccharomyces cerevisiae...
May 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28504211/ectopic-adenine-nucleotide-translocase-activity-controls-extracellular-adp-levels-and-regulates-the-f1-atpase-mediated-hdl-endocytosis-pathway-on-hepatocytes
#20
G Cardouat, T Duparc, S Fried, B Perret, S Najib, L O Martinez
Ecto-F1-ATPase is a complex related to mitochondrial ATP synthase which has been identified as a plasma membrane receptor for apolipoprotein A-I (apoA-I), the major protein of high-density lipoprotein (HDL), and has been shown to contribute to HDL endocytosis in several cell types. On hepatocytes, apoA-I binding to ecto-F1-ATPase stimulates extracellular ATP hydrolysis into ADP, which subsequently activates a P2Y13-mediated HDL endocytosis pathway. Interestingly, other mitochondrial proteins have been found to be expressed at the plasma membrane of several cell types...
May 11, 2017: Biochimica et Biophysica Acta
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