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https://www.readbyqxmd.com/read/29338039/stat3-expression-by-myeloid-cells-is-detrimental-for-the-t-cell-mediated-control-of-infection-with-mycobacterium-tuberculosis
#1
Yu Gao, Juan Ignacio Basile, Cajsa Classon, Dolores Gavier-Widen, Akihiko Yoshimura, Berit Carow, Martin E Rottenberg
STAT3 is a master regulator of the immune responses. Here we show that M. tuberculosis-infected stat3fl/fl lysm cre mice, defective for STAT3 in myeloid cells, contained lower bacterial load in lungs and spleens, reduced granuloma extension but higher levels of pulmonary neutrophils. STAT3-deficient macrophages showed no improved control of intracellular mycobacterial growth. Instead, protection associated to elevated ability of stat3fl/fl lysm cre antigen-presenting cells (APCs) to release IL-6 and IL-23 and to stimulate IL-17 secretion by mycobacteria-specific T cells...
January 16, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29299716/delivery-of-helicobacter-pylori-hpaa-to-gastrointestinal-mucosal-immune-sites-using-lactococcus-lactis-and-its-immune-efficacy-in-mice
#2
Rongguang Zhang, Chen Wang, Wenbin Cheng, Guangcai Duan, Qingfeng Shi, Shuaiyin Chen, Qingtang Fan
OBJECTIVE: To develop a safe and effective oral vaccine against Helicobacter pylori using its HpaA protein expressed in Lactococcus lactis. RESULTS: The gene encoding HpaA was obtained by PCR and ligated to pNZ8110-lysM following digestion with NaeI + SphI. The recombinant plasmid was transferred into E. coli for multiplication, and then into L. lactis. The recombinant L. lactis was induced to express HpaA, resulting in two products of 29 and 25 kDa, both of which yielded positive immunoreaction with mouse antisera against H...
January 3, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29287721/intravital-imaging-of-neutrophil-recruitment-in-intestinal-ischemia-reperfusion-injury
#3
Shintaro Hashimoto, Masaki Honda, Takayuki Takeichi, Masataka Sakisaka, Yasuko Narita, Daiki Yoshii, Keiichi Uto, Seisuke Sakamoto, Yukihiro Inomata
BACKGROUND: Neutrophils are known to be key players in innate immunity. Activated neutrophils induce local inflammation, which results in pathophysiologic changes during intestinal ischemia-reperfusion injury (IRI). However, most studies have been based on static assessments, and few have examined real-time intravital neutrophil recruitment. We herein report a method for imaging and evaluating dynamic changes in the neutrophil recruitment in intestinal IRI using two-photon laser scanning microscopy (TPLSM)...
January 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29247646/incomplete-clearance-of-apoptotic-cells-by-core-1-derived-o-glycan-deficient-resident-peritoneal-macrophages
#4
Hiromasa Wakui, Sayaka Fuseya, Riku Suzuki, Miki Shimbo, Risa Okada, Mitchito Hamada, Akihiro Kuno, Kozue Hagiwara, Takashi Sato, Hisashi Narimatsu, Takashi Kudo, Satoru Takahashi
The core 1 β1,3-galactosyltransferase-specific molecular chaperon (Cosmc) is essential for the synthesis of the core 1 structure of mucin-type O-glycans. To clarify the physiological role of core 1-derived O-glycans in macrophages, we exploited the LysM-Cre transgene to generate a conditional Cosmc mutant allele (conditional Cosmc knockout; cKO) in myeloid cells. cKO mice developed normally with no gross phenotypic abnormalities or abnormal peripheral blood counts. Resident peritoneal macrophages (rpMacs) of cKO mice exhibited impaired engulfment of apoptotic cells but showed normal macrophage differentiation and counts...
December 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29225599/promoter-specificity-and-efficacy-in-conditional-and-inducible-transgenic-targeting-of-lung-macrophages
#5
Alexandra L McCubbrey, Kristen C Allison, Alisa B Lee-Sherick, Claudia V Jakubzick, William J Janssen
Conditional and inducible Cre-loxP systems are used to target gene deletion to specific cell lineages and tissues through promoter-restricted expression of the bacterial DNA recombinase, Cre. Although Cre-loxP systems are widely used to target gene deletion in lung macrophages, limited data are published on the specificity and efficiency of "macrophage targeting" Cre lines. Using R26-stopfl/fl-TdTomato and tetOn-GFP reporter lines, we assessed the specificity and efficiency of four commercially available Cre driver lines that are often considered "macrophage specific...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29201496/platelets-prime-hematopoietic-and-vascular-niche-to-drive-angiocrine-mediated-liver-regeneration
#6
Koji Shido, Deebly Chavez, Zhongwei Cao, Jane Ko, Shahin Rafii, Bi-Sen Ding
In mammals, the livers regenerate after chemical injury or resection of hepatic lobe by hepatectomy. How liver regeneration is initiated after mass loss remains to be defined. Here, we report that following liver injury, activated platelets deploy SDF-1 and VEGF-A to stimulate CXCR7+ liver sinusoidal endothelial cell (LSEC) and VEGFR1+ myeloid cell, orchestrating hepatic regeneration. After carbon tetrachloride (CCl4) injection or hepatectomy, platelets and CD11b+VEGFR1+ myeloid cells were recruited LSEC, and liver regeneration in both models was impaired in thrombopoietin-deficient (Thpo-/-) mice lacking circulating platelets...
2017: Signal Transduction and Targeted Therapy
https://www.readbyqxmd.com/read/29194635/oscerk1-plays-a-crucial-role-in-the-lipopolysaccharide-induced-immune-response-of-rice
#7
Yoshitake Desaki, Yusuke Kouzai, Yusuke Ninomiya, Ryosuke Iwase, Yumi Shimizu, Keito Seko, Antonio Molinaro, Eiichi Minami, Naoto Shibuya, Hanae Kaku, Yoko Nishizawa
Plant cell surface receptor-like kinases (RLKs) mediate the signals from microbe-associated molecular patterns (MAMPs) that induce immune responses. Lipopolysaccharide (LPS), the major constituent of the outer membrane of gram-negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co-receptors are unknown except for LORE, a bulb-type lectin S-domain RLK (B-lectin SD1-RLK) in Arabidopsis. OsCERK1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP, and peptidoglycan, a bacterial MAMP...
November 30, 2017: New Phytologist
https://www.readbyqxmd.com/read/29184566/selection-signatures-in-the-first-exon-of-paralogous-receptor-kinase-genes-from-the-sym2-region-of-the-pisum-sativum-l-genome
#8
Anton S Sulima, Vladimir A Zhukov, Alexey A Afonin, Aleksandr I Zhernakov, Igor A Tikhonovich, Ludmila A Lutova
During the initial step of the symbiosis between legumes (Fabaceae) and nitrogen-fixing bacteria (rhizobia), the bacterial signal molecule known as the Nod factor (nodulation factor) is recognized by plant LysM motif-containing receptor-like kinases (LysM-RLKs). The fifth chromosome of barrel medic (Medicago truncatula Gaertn.) contains a cluster of paralogous LysM-RLK genes, one of which is known to participate in symbiosis. In the syntenic region of the pea (Pisum sativum L.) genome, three genes have been identified: PsK1 and PsSym37, two symbiosis-related LysM-RLK genes with known sequences, and the unsequenced PsSym2 gene which presumably encodes a LysM-RLK and is associated with increased selectivity to certain Nod factors...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29183785/ablation-of-gs%C3%AE-signaling-in-osteoclast-progenitor-cells-adversely-affects-skeletal-bone-maintenance
#9
REVIEW
Girish Ramaswamy, John Fong, Niambi Brewer, Hyunsoo Kim, Deyu Zhang, Yongwon Choi, Frederick S Kaplan, Eileen M Shore
Gsα, the alpha stimulatory subunit of heterotrimeric G proteins that activates downstream signaling through the adenylyl cyclase and cAMP/PKA pathway, plays an important role in bone development and remodeling. The role of Gsα in mesenchymal stem cell (MSC) differentiation to osteoblasts has been demonstrated in several mouse models of Gsα inactivation. Previously, using mice with heterozygous germline deletion of Gsα (Gnas+/p-), we identified a novel additional role for Gsα in bone remodeling, and showed the importance of Gnas in maintaining bone quality by regulating osteoclast differentiation and function...
November 25, 2017: Bone
https://www.readbyqxmd.com/read/29182677/arabidopsis-ubiquitin-ligase-pub12-interacts-with-and-negatively-regulates-chitin-elicitor-receptor-kinase-1-cerk1
#10
Koji Yamaguchi, Hirohisa Mezaki, Masayuki Fujiwara, Yuki Hara, Tsutomu Kawasaki
In Arabidopsis, fungal chitin is recognized as a pathogen-associated molecular pattern (PAMP) by the chitin receptor complex containing the lysin-motif (LysM) receptor-like kinases CERK1 and LYK5. Upon the perception of chitin, CERK1 phosphorylates the receptor-like cytoplasmic kinase, PBL27, which activates the intracellular mitogen-activated protein kinase (MAPK) cascade. However, the mechanisms by which the CERK1-PBL27 complex is regulated remain largely unknown. We identified ubiquitin ligase PUB12 as a component of the PBL27 complex using co-immunoprecipitation and mass spectrometry...
2017: PloS One
https://www.readbyqxmd.com/read/29180421/metal-transporter-slc39a10-regulates-susceptibility-to-inflammatory-stimuli-by-controlling-macrophage-survival
#11
Hong Gao, Lu Zhao, Hao Wang, Enjun Xie, Xinhui Wang, Qian Wu, Yingying Yu, Xuyan He, Hongbin Ji, Lothar Rink, Junxia Min, Fudi Wang
Zn plays a key role in controlling macrophage function during an inflammatory event. Cellular Zn homeostasis is regulated by two families of metal transporters, the SLC39A family of importers and the SLC30A family of exporters; however, the precise role of these transporters in maintaining macrophage function is poorly understood. Using macrophage-specific Slc39a10-knockout (Slc39a10fl/fl;LysM-Cre+ ) mice, we found that Slc39a10 plays an essential role in macrophage survival by mediating Zn homeostasis in response to LPS stimulation...
December 5, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29171909/lys12-lysm-receptor-decelerates-phytophthora-palmivora-disease-progression-in-lotus-japonicus
#12
Winnie Fuechtbauer, Temur Yunusov, Zoltán Bozsóki, Aleksandr Gavrin, Euan K James, Jens Stougaard, Sebastian Schornack, Simona Radutoiu
Phytophthora palmivora is a devastating oomycete plant pathogen. We found that P. palmivora induces disease in Lotus japonicus and used this interaction to identify cellular and molecular events in response to this broad host range oomycete. Transcript quantification revealed that Lys12 was highly and rapidly induced during P. palmivora infection. Mutants of Lys12 displayed an accelerated disease progression, earlier plant death, and lower level of defense gene expression, while the defense program after chitin, laminarin, oligogalacturonide or flg22 treatment, or the root symbioses with nitrogen-fixing rhizobia and arbuscular mycorrhiza were similar to wild-type...
November 24, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29149533/monocyte-specific-knockout-of-c-ebp%C3%AE-results-in-osteopetrosis-phenotype-blocks-bone-loss-in-ovariectomized-mice-and-reveals-an-important-function-of-c-ebp%C3%AE-in-osteoclast-differentiation-and-function
#13
Wei Chen, Guochun Zhu, Joel Jules, Diep Nguyen, Yi-Ping Li
CCAAT/enhancer-binding protein α (C/ebpα) is critical for osteoclastogenesis by regulating osteoclast (OC) lineage commitment and is also important for OC differentiation and function in vitro. However, the role of C/ebpα in postnatal skeletal development has not been reported owing to lethality in C/ebpα(-/-) mice from hypoglycemia within 8 hours after birth. Herein, we generated conditional knockout mice by deleting the C/ebpα gene in monocyte via LysM-Cre to examine its role in OC differentiation and function...
November 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29122767/deficiency-in-protein-tyrosine-phosphatase-ptp1b-shortens-lifespan-and-leads-to-development-of-acute-leukemia
#14
Samantha Le Sommer, Nicola Morrice, Martina Pesaresi, Dawn Thompson, Mark A Vickers, Graeme I Murray, Nimesh Mody, Benjamin G Neel, Kendra K Bence, Heather M Wilson, Mirela Delibegovic
Protein tyrosine phosphatase PTP1B is a critical regulator of signaling pathways controlling metabolic homeostasis, cell proliferation and immunity. In this study, we report that global or myeloid-specific deficiency of PTP1B in mice decreases lifespan. We demonstrate that myeloid-specific deficiency of PTP1B is sufficient to promote the development of acute myeloid leukemia (AML). LysM-PTP1B-/- mice lacking PTP1B in the innate myeloid cell lineage displayed a dysregulation of bone marrow cells with a rapid decline in population at midlife and a concomitant increase in peripheral blood blast cells...
November 9, 2017: Cancer Research
https://www.readbyqxmd.com/read/29117277/naringenin-eye-drops-inhibit-corneal-neovascularization-by-anti-inflammatory-and-antioxidant-mechanisms
#15
Ana P M T Oguido, Miriam S N Hohmann, Felipe A Pinho-Ribeiro, Jefferson Crespigio, Talita P Domiciano, Waldiceu A Verri, Antonio M B Casella
Purpose: To investigate the effect of naringenin eye drops in corneal neovascularization induced by alkali (1 N NaOH) burn in mice. Methods: Corneal neovascularization in the right eye of male Swiss mice was induced by alkali. Treatment with naringenin eye drops (0.08-80 μg; 8 μL of 0.01-10 g/L solution) or vehicle (saline) started 2 days before corneal neovascularization was induced and was performed twice a day. Mice were treated up until the time animals were euthanized and cornea tissue was collected for testing, which was 2, 4, and 6 hours after alkali stimulus for cytokine and antioxidant capacity measurements, and 3 and/or 7 days after alkali stimulus for the assessment of corneal epithelial thickness and neovascularization, neutrophil, and macrophage recruitment, and vascular endothelial growth factor (Vegf), platelet-derived growth factor (Pdgf), matrix metalloproteinase-14 (Mmp14), and pigment epithelium-derived factor (Pedf) mRNA expression...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29114065/bim-suppresses-the-development-of-sle-by-limiting-myeloid-inflammatory-responses
#16
FuNien Tsai, Philip J Homan, Hemant Agrawal, Alexander V Misharin, Hiam Abdala-Valencia, G Kenneth Haines, Salina Dominguez, Christina L Bloomfield, Rana Saber, Anthony Chang, Chandra Mohan, Jack Hutcheson, Anne Davidson, G R Scott Budinger, Philippe Bouillet, Andrea Dorfleutner, Christian Stehlik, Deborah R Winter, Carla M Cuda, Harris Perlman
The Bcl-2 family is considered the guardian of the mitochondrial apoptotic pathway. We demonstrate that Bim acts as a molecular rheostat by controlling macrophage function not only in lymphoid organs but also in end organs, thereby preventing the break in tolerance. Mice lacking Bim in myeloid cells (LysM(Cre)Bim(fl/fl)) develop a systemic lupus erythematosus (SLE)-like disease that mirrors aged Bim(-/-) mice, including loss of marginal zone macrophages, splenomegaly, lymphadenopathy, autoantibodies (including anti-DNA IgG), and a type I interferon signature...
November 7, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29105533/plant-immunity-and-symbiosis-signaling-mediated-by-lysm-receptors
#17
Yoshitake Desaki, Kana Miyata, Maruya Suzuki, Naoto Shibuya, Hanae Kaku
Plants possess the ability to recognize microbe-associated molecular patterns (MAMPs) and PAMPs through the PRRs, and initiate pattern-triggered immunity. MAMPs are derived from cell-envelope components, secreted materials and cytosolic proteins from bacteria, oomycetes or fungi, and some MAMPs play a similar function in the innate immunity in mammals. Chitin is a representative fungal MAMP and triggers defense signaling in a wide range of plant species. The chitin receptors CEBiP and CERK1 on the plasma membrane have LysM (lysin motif) in their ectodomains...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/29084256/identification-and-characterization-of-lysm-effectors-in-penicillium-expansum
#18
Elena Levin, Ana Rosa Ballester, Ginat Raphael, Oleg Feigenberg, Yongsheng Liu, John Norelli, Luis Gonzalez-Candelas, Jing Ma, Christopher Dardick, Michael Wisniewski, Samir Droby
P. expansum is regarded as one of the most important postharvest rots of apple fruit and is also of great concern to fruit processing industries. Elucidating the pathogenicity mechanism of this pathogen is of utmost importance for the development of effective and safe management strategies. Although, many studies on modification of the host environment by the pathogen were done, its interactions with fruit during the early stages of infection and the virulence factors that mediate pathogenicity have not been fully defined...
2017: PloS One
https://www.readbyqxmd.com/read/29083116/non-branched-%C3%AE-1-3-glucan-oligosaccharides-trigger-immune-responses-in-arabidopsis
#19
Hugo Mélida, Sara Sopeña-Torres, Laura Bacete, María Garrido-Arandia, Lucía Jordá, Gemma López, Antonio Muñoz, Luis F Pacios, Antonio Molina
Fungal cell walls, essential for environmental adaptation and host colonization, have been evolutionarily selected by plants and animals as a source of Microbe-Associated Molecular Patterns (MAMPs) that upon recognition by host Pattern Recognition Receptors (PRRs) trigger immune responses conferring disease resistance. Chito-oligosaccharides (β-1,4-N-acetylglucosamine oligomers, (GlcNAc)n ) are the only glycosidic structures from fungal walls well-shown to function as MAMPs in plants. Perception of (GlcNAc)4-8 by Arabidopsis involves CERK1, LYK4 and LYK5, three of the eight members of LysM PRR family...
October 30, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29081780/deletion-of-the-mineralocorticoid-receptor-in-myeloid-cells-attenuates-central-nervous-system-autoimmunity
#20
Elena Montes-Cobos, Nils Schweingruber, Xiao Li, Henrike J Fischer, Holger M Reichardt, Fred Lühder
Myeloid cells play an important role in the pathogenesis of multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Monocytes, macrophages, and microglia can adopt two distinct phenotypes, with M1-polarized cells being more related to inflammation and autoimmunity while M2-polarized cells contribute to tissue repair and anti-inflammatory processes. Here, we show that deletion of the mineralocorticoid receptor (MR) in bone marrow-derived macrophages and peritoneal macrophages caused their polarization toward the M2 phenotype with its distinct gene expression, altered phagocytic and migratory properties, and dampened NO production...
2017: Frontiers in Immunology
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