keyword
https://read.qxmd.com/read/37340175/local-administration-of-liposomal-based-plekhf1-gene-therapy-attenuates-pulmonary-fibrosis-by-modulating-macrophage-polarization
#21
JOURNAL ARTICLE
Lifeng Yan, Chenchen Hou, Juan Liu, Yi Wang, Chenxi Zeng, Jun Yu, Tianyu Zhou, Qing Zhou, Shengzhong Duan, Weining Xiong
Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease with limited therapeutic options. Macrophages, particularly alternatively activated macrophages (M2), have been recognized to contribute to the pathogenesis of pulmonary fibrosis. Therefore, targeting macrophages might be a viable therapeutic strategy for IPF. Herein, we report a potential nanomedicine-based gene therapy for IPF by modulating macrophage M2 activation. In this study, we illustrated that the levels of pleckstrin homology and FYVE domain containing 1 (Plekhf1) were increased in the lungs originating from IPF patients and PF mice...
June 16, 2023: Science China. Life Sciences
https://read.qxmd.com/read/37276818/recent-progress-in-type%C3%A2-1-classical-dendritic-cell-cross-presentation-cytosolic-vacuolar-or-both
#22
REVIEW
Ray A Ohara, Kenneth M Murphy
Type 1 classical dendritic cells (cDC1s) have emerged as the major antigen-presenting cell performing cross-presentation (XP) in vivo, but the antigen-processing pathway in this cell remains obscure. Two competing models for in vivo XP of cell-associated antigens by cDC1 include a vacuolar pathway and cytosolic pathway. A vacuolar pathway relies on directing antigens captured in vesicles toward a class I major histocompatibility complex loading compartment independently of cytosolic entry. Alternate proposals invoke phagosomal rupture, either constitutive or triggered by spleen tyrosine kinase (SYK) signaling in response to C-type lectin domain family 9 member A (CLEC9A) engagement, that releases antigens into the cytosol for proteasomal degradation...
June 3, 2023: Current Opinion in Immunology
https://read.qxmd.com/read/37248430/intermittent-inhibition-of-fyve-finger-containing-phosphoinositide-kinase-induces-melanosome-degradation-in-b16f10-melanoma-cells
#23
JOURNAL ARTICLE
Kyoka Kawaguchi, Miyu Watanabe, Saho Furukawa, Kenichi Koga, Hiromitsu Kanamori, Mitsushi J Ikemoto, Shigeo Takashima, Miwa Maeda, Kentaro Oh-Hashi, Yoko Hirata, Kyoji Furuta, Hiroshi Takemori
BACKGROUND: Melanosomes are lysosome-related organelles that contain melanogenic factors and synthesize melanin as they mature. FYVE finger-containing phosphoinositide kinase (PIKfyve) regulates late endosome and lysosome morphology, vesicle trafficking, and autophagy. In melanocytes, PIKfyve inhibition has been reported to induce hypopigmentation due to impairments in the metabolism of early-stage melanosomes. METHODS AND RESULTS: Here, we report a new type of melanosome metabolism: post-PIKfyve inhibition, which was found during the characterization of the endosome/lysosome fluoroprobe GIF-2250...
May 29, 2023: Molecular Biology Reports
https://read.qxmd.com/read/37175801/phafins-are-more-than-phosphoinositide-binding-proteins
#24
REVIEW
Tuoxian Tang, Mahmudul Hasan, Daniel G S Capelluto
Phafins are PH (Pleckstrin Homology) and FYVE (Fab1, YOTB, Vac1, and EEA1) domain-containing proteins. The Phafin protein family is classified into two groups based on their sequence homology and functional similarity: Phafin1 and Phafin2. This protein family is unique because both the PH and FYVE domains bind to phosphatidylinositol 3-phosphate [PtdIns(3)P], a phosphoinositide primarily found in endosomal and lysosomal membranes. Phafin proteins act as PtdIns(3)P effectors in apoptosis, endocytic cargo trafficking, and autophagy...
April 30, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37095576/a-myeloid-leukemia-factor-homolog-is-involved-in-tolerance-to-stresses-and-stress-induced-protein-metabolism-in-giardia-lamblia
#25
JOURNAL ARTICLE
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
BACKGROUND: The eukaryotic membrane vesicles contain specific sets of proteins that determine vesicle function and shuttle with specific destination. Giardia lamblia contains unknown cytosolic vesicles that are related to the identification of a homolog of human myeloid leukemia factor (MLF) named MLF vesicles (MLFVs). Previous studies suggest that MLF also colocalized with two autophagy machineries, FYVE and ATG8-like protein, and that MLFVs are stress-induced compartments for substrates of the proteasome or autophagy in response to rapamycin, MG132, and chloroquine treatment...
April 24, 2023: Biology Direct
https://read.qxmd.com/read/36917659/in-situ-snapshots-along-a-mammalian-selective-autophagy-pathway
#26
JOURNAL ARTICLE
Meijing Li, Ishita Tripathi-Giesgen, Brenda A Schulman, Wolfgang Baumeister, Florian Wilfling
Selective macroautophagy (hereafter referred to as autophagy) describes a process in which cytosolic material is engulfed in a double membrane organelle called an autophagosome. Autophagosomes are carriers responsible for delivering their content to a lytic compartment for destruction. The cargo can be of diverse origin, ranging from macromolecular complexes to protein aggregates, organelles, and even invading pathogens. Each cargo is unique in composition and size, presenting different challenges to autophagosome biogenesis...
March 21, 2023: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/36884084/gwas-scans-of-cereal-cyst-nematode-heterodera-avenae-resistance-in-indian-wheat-germplasm
#27
JOURNAL ARTICLE
Vikas Kumar Singh, Deepti Chaturvedi, Saksham Pundir, Deepak Kumar, Rajiv Sharma, Sundeep Kumar, Shiveta Sharma, Shailendra Sharma
Significant yield losses in major cereal-growing regions around the world have been linked to cereal cyst nematodes (Heterodera spp.). Identifying and deploying natural sources of resistance is of utmost importance due to increasing concerns associated with chemical methods over the years. We screened 141 diverse wheat genotypes collected from pan-Indian wheat cultivation states for nematode resistance over two years, alongside two resistant (Raj MR1, W7984 (M6)) and two susceptible (WH147, Opata M85) checks...
March 8, 2023: Molecular Genetics and Genomics: MGG
https://read.qxmd.com/read/36780172/circ_0115118-regulates-endometrial-functions-through-the-mir-138-1-3p-wdfy2-axis-in-patients-with-pcos
#28
JOURNAL ARTICLE
Zi Yang, Fenting Liu, Jiali Bai, Zhenhong Ye, Jingwen Yin, Tianliu Peng, Hongying Shan, Yang Yu, Ping Zhou, Rong Li
PURPOSE: To investigate the expression profiles of circular RNAs (circRNAs) in the endometria of women with polycystic ovary syndrome (PCOS) and to explore the role of aberrant circ_0115118 expression in endometrial dysfunction in patients with PCOS. METHODS: In this study, circRNA microarray hybridization and bioinformatic analyses were performed to determine the expression patterns of circRNAs in the endometria of patients with or without PCOS, and the expression of target circRNA was evaluated by real-time PCR...
February 13, 2023: Biology of Reproduction
https://read.qxmd.com/read/36709383/transcription-factor-eb-regulates-phosphatidylinositol-3-phosphate-levels-that-control-lysosome-positioning-in-the-bladder-cancer-model
#29
JOURNAL ARTICLE
Pallavi Mathur, Camilla De Barros Santos, Hugo Lachuer, Julie Patat, Bruno Latgé, François Radvanyi, Bruno Goud, Kristine Schauer
Lysosomes orchestrate degradation and recycling of exogenous and endogenous material thus controlling cellular homeostasis. Little is known how this organelle changes during cancer. Here we investigate the intracellular landscape of lysosomes in a cellular model of bladder cancer. Employing standardized cell culture on micropatterns we identify a phenotype of peripheral lysosome positioning prevailing in bladder cancer cell lines but not normal urothelium. We show that lysosome positioning is controlled by phosphatidylinositol-3-phosphate (PtdIns3P) levels on endomembranes which recruit FYVE-domain containing proteins for lysosomal dispersion...
January 28, 2023: Communications Biology
https://read.qxmd.com/read/36695658/inhibition-of-pikfyve-kinase-interferes-escrt-pathway-to-suppress-rna-virus-replication
#30
JOURNAL ARTICLE
Zhen Luo, Yicong Liang, Mingfu Tian, Zhihui Ruan, Rui Su, Muhammad Adnan Shereen, Jialing Yin, Kailang Wu, Jun Guo, Qiwei Zhang, Yongkui Li, Jianguo Wu
ESCRT (Endosomal sorting complex required for transport) is essential in the functional operation of endosomal transport in envelopment and budding of enveloped RNA viruses. However, in non-enveloped RNA viruses such as enteroviruses of the Picornaviridae family, the precise function of ESCRT pathway in viral replication remains elusive. Here, we initially evaluated that ESCRT pathway is important for viral replication upon EV71 (Enterovirus 71) infection. Furthermore, we discovered that YM201636, a specific inhibitor of PIKFYVE (Phosphoinositide kinase, FYVE finger containing) kinase, significantly suppressed EV71 replication and virus-induced inflammation in vitro and in vivo...
January 25, 2023: Journal of Medical Virology
https://read.qxmd.com/read/36642112/exosomal-mir-211-5p-regulates-glucose-metabolism-pyroptosis-and-immune-microenvironment-of-melanoma-through-gna15
#31
JOURNAL ARTICLE
Bin Zeng, Yuting Chen, Hao Chen, Qiting Zhao, Zhiwei Sun, Doudou Liu, Xiaoshuang Li, Yuhan Zhang, Jianyu Wang, H Rosie Xing
Despite the unprecedented advancement of cancer treatment, the prognosis for patients with metastatic stage of cancer remains poor. The challenge that underlines this clinical dilemma is the complexity of metastasis. The conventional experiment-driven discovery approaches (the "wet lab") yield overly simplified one-to-one mechanistic relationships that are inept of elucidating the complexity of metastasis. Metastasis research also suffers from the knowledge and skill deficiency of the individual investigators...
January 12, 2023: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://read.qxmd.com/read/36582637/alkaline-stress-reduces-root-waving-by-regulating-pin7-vacuolar-transport
#32
JOURNAL ARTICLE
Yu Liu, Chenglin Mu, Dongdong Du, Yi Yang, Lixin Li, Wei Xuan, Stefan Kircher, Klaus Palme, Xugang Li, Ruixi Li
Root development and plasticity are assessed via diverse endogenous and environmental cues, including phytohormones, nutrition, and stress. In this study, we observed that roots in model plant Arabidopsis thaliana exhibited waving and oscillating phenotypes under normal conditions but lost this pattern when subjected to alkaline stress. We later showed that alkaline treatment disturbed the auxin gradient in roots and increased auxin signal in columella cells. We further demonstrated that the auxin efflux transporter PIN-FORMED 7 (PIN7) but not PIN3 was translocated to vacuole lumen under alkaline stress...
2022: Frontiers in Plant Science
https://read.qxmd.com/read/36574842/the-ntpase-activity-of-the-double-fyve-domain-containing-protein-1-dfcp1-regulates-lipid-droplet-metabolism
#33
JOURNAL ARTICLE
V A Ismail, T Naismith, D J Kast
Lipid droplets (LDs) are transient lipid storage organelles that can be readily tapped to resupply cells with energy or lipid building blocks, and therefore play a central role in cellular metabolism. However, the molecular factors and underlying mechanisms that regulate the growth and degradation of LDs are poorly understood. It has emerged that proteins that establish contacts between LDs and the ER play a critical role in regulating LD metabolism. Recently, the autophagy related protein, Double FYVE Domain Containing Protein 1 (DFCP1/ZFYV1) was shown to reside at the interface of the ER and LDs, however, little is known about the involvement of DFCP1 in autophagy and LD metabolism...
December 24, 2022: Journal of Biological Chemistry
https://read.qxmd.com/read/36548271/metabolic-differences-and-differentially-expressed-genes-between-c57bl-6j-and-c57bl-6n-mice-substrains
#34
JOURNAL ARTICLE
Shino Nemoto, Tetsuya Kubota, Hiroshi Ohno
C57BL/6J (B6J) and C57BL/6N (B6N) mice are the most frequently used substrains in C57BL/6 (B6) inbred mice, serving as physiological models for in vivo studies and as background strains to build transgenic mice. However, the differences in metabolic phenotypes between B6J and B6N mice are not coherent, and genotypic differences in metabolically important tissues have not been well studied. The phenotypic differences between B6J and B6N substrains have often been attributed to the role of the nicotinamide nucleotide transhydrogenase (Nnt) gene, whereby B6J has a spontaneous missense mutation of Nnt...
2022: PloS One
https://read.qxmd.com/read/36470866/pexophagy-suppresses-ros-induced-damage-in-leaf-cells-under-high-intensity-light
#35
JOURNAL ARTICLE
Kazusato Oikawa, Shino Goto-Yamada, Yasuko Hayashi, Daisuke Takahashi, Yoshitaka Kimori, Michitaro Shibata, Kohki Yoshimoto, Atsushi Takemiya, Maki Kondo, Kazumi Hikino, Akira Kato, Keisuke Shimoda, Haruko Ueda, Matsuo Uemura, Keiji Numata, Yoshinori Ohsumi, Ikuko Hara-Nishimura, Shoji Mano, Kenji Yamada, Mikio Nishimura
Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes...
December 5, 2022: Nature Communications
https://read.qxmd.com/read/36461945/the-phytophthora-sojae-effector-psfyve1-modulates-immunity-related-gene-expression-by-targeting-host-rz-1a-protein
#36
JOURNAL ARTICLE
Xinyu Lu, Zitong Yang, Wen Song, Jinlu Miao, Hanqing Zhao, Peiyun Ji, Tianli Li, Jierui Si, Zhiyuan Yin, Maofeng Jing, Danyu Shen, Daolong Dou
Oomycete pathogens secrete numerous effectors to manipulate plant immunity and promote infection. However, relatively few effector types have been well characterized. In this study, members of a FYVE domain-containing protein family that is highly expanded in oomycetes were systematically identified, and one secreted protein, PsFYVE1, was selected for further study. PsFYVE1 enhanced Phytophthora capsici infection in Nicotiana benthamiana and was necessary for Phytophthora sojae virulence. The FYVE domain of PsFYVE1 had PI3P-binding activity that depended on four conserved amino acid residues...
December 3, 2022: Plant Physiology
https://read.qxmd.com/read/36422153/transmembrane-membrane-readers-form-a-novel-class-of-proteins-that-include-peripheral-phosphoinositide-recognition-domains-and-viral-spikes
#37
REVIEW
Michael Overduin, Anh Tran, Dominic M Eekels, Finn Overduin, Troy A Kervin
Membrane proteins are broadly classified as transmembrane (TM) or peripheral, with functions that pertain to only a single bilayer at a given time. Here, we explicate a class of proteins that contain both transmembrane and peripheral domains, which we dub transmembrane membrane readers (TMMRs). Their transmembrane and peripheral elements anchor them to one bilayer and reversibly attach them to another section of bilayer, respectively, positioning them to tether and fuse membranes while recognizing signals such as phosphoinositides (PIs) and modifying lipid chemistries in proximity to their transmembrane domains...
November 17, 2022: Membranes
https://read.qxmd.com/read/36373807/the-plant-fyve-domain-containing-protein-free1-associates-with-microprocessor-components-to-repress-mirna-biogenesis
#38
JOURNAL ARTICLE
Hongbo Li, Tingting Li, Yingzhu Li, Haiyan Bai, Yanghuan Dai, Yanglan Liao, Juan Wei, Wenjin Shen, Binglian Zheng, Zhonghui Zhang, Caiji Gao
FYVE domain protein required for endosomal sorting 1 (FREE1), originally identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT) machinery, plays diverse roles either in endosomal sorting in the cytoplasm or in transcriptional regulation of abscisic acid signaling in the nucleus. However, to date, a role for FREE1 or other ESCRT components in the regulation of plant miRNA biology has not been discovered. Here, we demonstrate a nuclear function of FREE1 as a cofactor in miRNA biogenesis in plants...
November 14, 2022: EMBO Reports
https://read.qxmd.com/read/36282215/rufy1-binds-arl8b-and-mediates-endosome-to-tgn-ci-m6pr-retrieval-for-cargo-sorting-to-lysosomes
#39
JOURNAL ARTICLE
Shalini Rawat, Dhruba Chatterjee, Rituraj Marwaha, Gitanjali Charak, Gaurav Kumar, Shrestha Shaw, Divya Khatter, Sheetal Sharma, Cecilia de Heus, Nalan Liv, Judith Klumperman, Amit Tuli, Mahak Sharma
Arl8b, an Arf-like GTP-binding protein, regulates cargo trafficking and positioning of lysosomes. However, it is unknown whether Arl8b regulates lysosomal cargo sorting. Here, we report that Arl8b binds to the Rab4 and Rab14 interaction partner, RUN and FYVE domain-containing protein (RUFY) 1, a known regulator of cargo sorting from recycling endosomes. Arl8b determines RUFY1 endosomal localization through regulating its interaction with Rab14. RUFY1 depletion led to a delay in CI-M6PR retrieval from endosomes to the TGN, resulting in impaired delivery of newly synthesized hydrolases to lysosomes...
January 2, 2023: Journal of Cell Biology
https://read.qxmd.com/read/36274208/two-new-variants-in-fyco1-are-responsible-for-autosomal-recessive-congenital-cataract-in-iranian-population
#40
JOURNAL ARTICLE
Ebrahim Shirzadeh, Fahimeh Piryaei, Hanieh Naddaf, Zahra Barabadi
The purpose of this experimental study was to investigate the genetic etiology of congenital cataract (CC) manifesting an autosomal recessive pattern of inheritance in four Iranian families. Affected individuals and their normal first-degree relatives in each family were included in the present study. The genomic DNA of the blood samples was extracted from all participants, and one affected member belonging to each family was subjected to Whole Exome Sequencing (WES). Using bidirectional Sanger sequencing, the identified variants were validated by co-segregation analysis...
September 12, 2022: Cell Journal
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