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https://www.readbyqxmd.com/read/28523344/role-of-g3bp1-in-glucocorticoid-receptor-mediated-microrna-15b-and-microrna-23a-biogenesis-in-endothelial-cells
#1
Hoi-Hin Kwok, Po-Ying Poon, Kylie Hin-Man Mak, Lin-Yao Zhang, Pei Liu, Huoming Zhang, Nai-Ki Mak, Patrick Ying-Kit Yue, Ricky Ngok-Shun Wong
MicroRNAs (miRNAs) are a family of non-coding RNAs that play crucial roles in regulating various normal cellular responses. Recent studies revealed that the canonical miRNA biogenesis pathway is subject to sophisticated regulation. Hormonal control of miRNA biogenesis by androgen and estrogen has been demonstrated, but the direct effects of the glucocorticoid receptor (GR) on miRNA biogenesis are unknown. This study revealed the role of GR in miRNA maturation. We showed that two GR agonists, dexamethasone and ginsenoside-Rg1 rapidly suppressed the expression of mature miR-15b, miR-23a, and miR-214 in human endothelial cells...
May 18, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28516119/affinity-pulldown-of-biotinylated-rna-for-detection-of-protein-rna-complexes
#2
Amaresh C Panda, Jennifer L Martindale, Myriam Gorospe
RNA-binding proteins (RBPs) have recently emerged as crucial players in the regulation of gene expression. The interactions of RBPs with target mRNAs control the levels of gene products by altering different regulatory steps, including pre-mRNA splicing and maturation, nuclear mRNA export, and mRNA stability and translation (Glisovic et al., 2008). There are several methodologies available today to identify RNAs bound to specific RBPs; some detect only recombinant molecules in vitro, others detect recombinant and endogenous molecules, while others detect only endogenous molecules...
December 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28502712/ligation-mediated-pcr-with-a-back-to-back-adapter-reduces-amplification-bias-resulting-from-variations-in-gc-content
#3
Satoru Ishihara, Naoe Kotomura, Naoki Yamamoto, Hiroshi Ochiai
Ligation-mediated polymerase chain reaction (LM-PCR) is a common technique for amplification of a pool of DNA fragments. Here, a double-stranded oligonucleotide consisting of two primer sequences in back-to-back orientation was designed as an adapter for LM-PCR. When DNA fragments were ligated with this adapter, the fragments were sandwiched between two adapters in random orientations. In the ensuing PCR, ligation products linked at each end to an opposite side of the adapter, i.e. to a distinct primer sequence, were preferentially amplified compared with products linked at each end to an identical primer sequence...
May 11, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28474873/circuit-strength-training-improves-muscle-strength-functional-performance-and-anthropometric-indicators-in-sedentary-elderly-women
#4
Mauro L Mazini Filho, Felipe J Aidar, Dihogo Gama de Matos, Osvaldo Costa Moreira, Cláudia E Patrocínio de Oliveira, Gabriela R de Oliveira Venturini, Victor Magalhães Curty, Henrique Menezes Touguinha, Maria E Caputo Ferreira
BACKGROUND: This study aimed to investigate the effects of circuit strength training on the muscle strength, functional autonomy and anthropometric indicators of the elderly. METHODS: Were included 65 women divided in two groups: strength training (TG, n= 34) and control group (CG, n = 31). The strength-training group was subjected to a circuit shaped training program, three days per week, for a period of 12 weeks. In each training session, the circuit was repeated three times...
April 26, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28472785/cdc5l-promotes-htert-expression-and-colorectal-tumor-growth
#5
Jia Li, Ningning Zhang, Rui Zhang, Longmei Sun, Wendan Yu, Wei Guo, Yingying Gao, Mei Li, Wei Liu, Pin Liang, Wuguo Deng, Xiaonan Cui
BACKGROUND/AIMS: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide because the survival rate remains low. Cell division cycle 5-like (CDC5L) is highly expressed in some cancer cells, but the mechanism requires clarification. Human telomerase reverse transcriptase (hTERT) plays important roles in CRC. METHODS: This study aimed to identify a link between CDC5L and hTERT and to determine their effects on the signaling pathways, migration and prognosis of CRC cells...
May 4, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28471450/ubiquitylation-of-mfhas1-by-the-ubiquitin-ligase-praja2-promotes-m1-macrophage-polarization-by-activating-jnk-and-p38-pathways
#6
Jing Zhong, Huihui Wang, Wankun Chen, Zhirong Sun, Jiawei Chen, Yajun Xu, Meilin Weng, Qiqing Shi, Duan Ma, Changhong Miao
Sepsis is a systemic inflammation caused by infection. The balance between M1-M2 macrophage polarization has an essential role in the pathogenesis of sepsis. However, the exact mechanism underlying macrophage polarization is unclear. We previously showed that levels of malignant fibrous histiocytoma amplified sequence 1 (MFHAS1) were significantly elevated in septic patients compared with those in nonseptic patients, and involved in the activation of Toll-like receptor (TLR) 2/c-Jun N-terminal kinase (JNK)/nuclear factor (NF)-κB pathway...
May 4, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28470345/lncrna-ccat1-inhibits-cell-apoptosis-of-renal-cell-carcinoma-through-up-regulation-of-livin-protein
#7
Shaoan Chen, Pengpeng Ma, Bin Li, Dawei Zhu, Xiude Chen, Yuzhu Xiang, Tengteng Wang, Xiangbin Ren, Chuan Liu, Xunbo Jin
This study was to investigate the involvement of long non-coding RNA (lncRNA) colon cancer-associated transcript-1 (CCAT1) in renal cell carcinoma (RCC) and to further uncover its underlying mechanism. In this study, the expression of CCAT1 and Livin of RCC tissues or cells was determined using qRT-PCR (quantitative real-time PCR) and western blot, respectively. RNA pulldown and RIP (RNA-Binding Protein Immunoprecipitation) assays were performed to examine the sequence interaction between CCAT1 and Livin. The viability and apoptosis of RCC cells was assessed by MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and TUNEL (TdT-mediated dUTP nick end labeling) assays, respectively...
May 3, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28458662/virf-relieves-the-transcriptional-attenuation-of-the-virulence-gene-icsa-of-shigella-flexneri-affecting-the-icsa-mrna-rnag-complex-formation
#8
Mara Giangrossi, Anna M Giuliodori, Chi N Tran, Augusto Amici, Cristina Marchini, Maurizio Falconi
VirF is the master activator of virulence genes of Shigella and its expression is required for the invasion of the human intestinal mucosa by pathogenic bacteria. VirF was shown to directly activate the transcription of virB and icsA, which encode two essential proteins involved in the pathogenicity process, by binding their promoter regions. In this study, we demonstrate by band shift, enzymatic probing and cross-linking experiments that VirF, in addition to DNA, can also bind the icsA transcript and RnaG, an antisense non-coding small RNA that promotes the premature termination of icsA mRNA through a transcriptional attenuation mechanism...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28446664/analysis-of-competing-hiv-1-splice-donor-sites-uncovers-a-tight-cluster-of-splicing-regulatory-elements-within-exon-2-2b
#9
Anna-Lena Brillen, Lara Walotka, Frank Hillebrand, Lisa Müller, Marek Widera, Stephan Theiss, Heiner Schaal
The HIV-1 accessory protein Vif is essential for viral replication by counteracting the host restriction factor APOBEC3G (A3G), and balanced levels of both proteins are required for efficient viral replication. Non-coding exons 2/2b contain the Vif start codon between their alternatively used splice donors 2 and 2b (D2 and D2b). For the vif mRNA, intron 1 must be removed, while intron 2 must be retained. Thus, splice acceptor 1 (A1) must be activated by U1 snRNP binding to either D2 or D2b, while splicing at D2 or D2b must be prevented...
April 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28420424/a-novel-lncrna-uc-134-represses-hepatocellular-carcinoma-progression-by-inhibiting-cul4a-mediated-ubiquitination-of-lats1
#10
Wen Ni, Yuqin Zhang, Zetao Zhan, Feng Ye, Yonghao Liang, Jing Huang, Keli Chen, Longhua Chen, Yi Ding
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, and tumor recurrence and metastasis are major factors that contribute to the poor outcome of patients with HCC. Long noncoding RNAs (lncRNAs) are known to regulate different tumorigenic processes, and a growing body of evidence indicates that Hippo kinase signaling is inactivated in many cancers. However, the upstream lncRNA regulators of Hippo kinase signaling in HCC are poorly understood. METHODS: Using a lncRNA microarray, we identified a novel lncRNA, uc...
April 19, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28396352/association-of-the-cold-shock-dead-box-rna-helicase-rhle-to-the-rna-degradosome-in-caulobacter-crescentus
#11
Angel A Aguirre, Alexandre M Vicente, Steven W Hardwick, Daniela M Alvelos, Ricardo R Mazzon, Ben F Luisi, Marilis V Marques
In diverse bacterial lineages, multi-enzyme assemblies have evolved that are central elements of RNA metabolism and RNA-mediated regulation. The aquatic, Gram-negative bacteria Caulobacter crescentus, which has been a model system for studying the bacterial cell cycle, has an RNA degradosome assembly that is formed by the endoribonuclease RNase E and includes the DEAD-box RNA helicase RhlB. Immunoprecipitations of extracts from cells expressing an epitope-tagged RNase E reveal that RhlE, another member of the DEAD-box helicase family, associates with the degradosome at temperatures below the optimum for growth...
April 10, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28381168/long-non-coding-rna-colon-cancer-associated-transcript-1-functions-as-a-competing-endogenous-rna-to-regulate-cyclin-dependent-kinase-1-expression-by-sponging-mir-490-3p-in-hepatocellular-carcinoma-progression
#12
Chunqing Dou, Liyuan Sun, Xin Jin, Mingming Han, Bao Zhang, Tao Li
Hepatocellular carcinoma is an aggressive neoplasm and is one of the most common human cancers. Recently, long non-coding RNAs have been demonstrated to participate in pathogenesis of many diseases including the progression in several cancers. In this study, we found that the long non-coding RNA colon cancer-associated transcript 1 was upregulated in hepatocellular carcinoma tissues (p < 0.05), and high colon cancer-associated transcript 1 expression level was positively associated with tumor volume (p < 0...
April 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28376558/pfap2tel-harbouring-a-non-canonical-dna-binding-ap2-domain-binds-to-plasmodium-falciparum-telomeres
#13
Miguel Sierra-Miranda, Shruthi-Sridhar Vembar, Dulce María Delgadillo, Pedro A Ávila-López, Abril-Marcela Herrera-Solorio, Daniela Lozano Amado, Miguel Vargas, Rosaura Hernandez-Rivas
The telomeres of the malaria parasite Plasmodium falciparum are essential not only for chromosome end maintenance during blood stage development in humans but also to generate genetic diversity by facilitating homologous recombination of subtelomeric, multigene virulence families such as var and rifin. However, other than the telomerase PfTERT, proteins that act at P. falciparum telomeres are poorly characterised. To isolate components that bind to telomeres, we performed oligonucleotide pulldowns and electromobility shift assays with a telomeric DNA probe and identified a non-canonical member of the ApiAP2 family of transcription factors, PfAP2Tel (encoded by PF3D7_0622900), as a component of the P...
April 4, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28362488/serotonin-transporter-associated-protein-complexes-are-enriched-in-synaptic-vesicle-proteins-and-proteins-involved-in-energy-metabolism-and-ion-homeostasis
#14
Jana Haase, Joanna Grudzinska-Goebel, Heidi Kaastrup Müller, Agnieszka Münster-Wandowski, Elysian Chow, Kieran Wynne, Zohreh Farsi, Johannes-Friedrich Zander, Gudrun Ahnert-Hilger
The serotonin transporter (SERT) mediates Na(+)-dependent high-affinity serotonin uptake and plays a key role in regulating extracellular serotonin concentration in the brain and periphery. To gain novel insight into SERT regulation, we conducted a comprehensive proteomics screen to identify components of SERT-associated protein complexes in the brain by employing three independent approaches. In vivo SERT complexes were purified from rat brain using an immobilized high-affinity SERT ligand, amino-methyl citalopram...
April 21, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28351900/long-noncoding-rna-mantis-facilitates-endothelial-angiogenic-function
#15
Matthias S Leisegang, Christian Fork, Ivana Josipovic, Florian Richter, Jens Preussner, Jiong Hu, Matthew J Miller, Jeremy N Epah, Patrick Hofmann, Stefan Günther, Franziska Moll, Chanil Valasarajan, Juliana Heidler, Yuliya Ponomareva, Thomas M Freiman, Lars Maegdefessel, Karl H Plate, Michel Mittelbronn, Shizuka Uchida, Carsten Künne, Konstantinos Stellos, Ralph T Schermuly, Norbert Weissmann, Kavi Devraj, Ilka Wittig, Reinier A Boon, Stefanie Dimmeler, Soni S Pullamsetti, Mario Looso, Francis J Miller, Ralf P Brandes
Background -The angiogenic function of endothelial cells is regulated by numerous mechanisms but the impact of long noncoding RNAs (lncRNAs) has hardly been studied. We set out to identify novel and functionally important endothelial lncRNAs. Methods -Epigenetically controlled lncRNAs in human umbilical vein endothelial cells (HUVEC) were searched by exon-array analysis after knockdown of the histone demethylase JARID1B. Molecular mechanisms were investigated by RNA Pulldown and Immunoprecipitation, Mass spectrometry, Micro-array, several knockdown approaches, CRIPSR-Cas9, Assay for Transposase-Accessible Chromatin-Sequencing and chromatin immunoprecipitation in HUVEC...
March 28, 2017: Circulation
https://www.readbyqxmd.com/read/28331099/interferon-inducible-oligoadenylate-synthetase-like-protein-acts-as-an-antiviral-effector-against-classical-swine-fever-virus-via-the-mda5-mediated-type-i-interferon-signaling-pathway
#16
Lian-Feng Li, Jiahui Yu, Yuexiu Zhang, Qian Yang, Yongfeng Li, Lingkai Zhang, Jinghan Wang, Su Li, Yuzi Luo, Yuan Sun, Hua-Ji Qiu
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which poses a serious threat to the global pig industry. Interferons (IFNs) and IFN-stimulated genes (ISGs) play a key role in host antiviral defense. We have previously screened the porcine 2' -5' -oligoadenylate synthase-like protein (pOASL) as a potential anti-CSFV ISG using a reporter CSFV. This study aimed to clarify the underlying antiviral mechanism of pOASL against CSFV. We confirmed that CSFV replication was significantly suppressed in lentivirus-delivered, pOASL-overexpressing PK-15 cells, whereas silencing the expression of endogenous pOASL by small interfering RNAs markedly enhanced CSFV growth...
March 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28326061/come-an-essential-response-regulator-negatively-regulates-the-expression-of-the-capsular-polysaccharide-locus-and-attenuates-the-bacterial-virulence-in-streptococcus-pneumoniae
#17
Yuqiang Zheng, Xuemei Zhang, Xiaofang Wang, Libin Wang, Jinghui Zhang, Yibing Yin
The capsular polysaccharide (CPS) of Streptococcus pneumoniae is the main virulence factors required for effective colonization and invasive disease. The capacity to regulate CPS production at the transcriptional level is critical for the survival of S. pneumoniae in different host niches, but little is known about the transcription regulators of cps locus. In the present study, we isolated and identified the response regulator ComE, the master competence switch in transformation of S. pneumoniae, as a transcriptional regulator of cps locus by DNA affinity chromatography-pulldown, MALDI-TOF mass spectrometry (MS) and electrophoretic mobility shift assay (EMSA)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28278510/autocrine-vegf-and-il-8-promote-migration-via-src-vav2-rac1-pak1-signaling-in-human-umbilical-vein-endothelial-cells
#18
Li Ju, Zhiwen Zhou, Bo Jiang, Yue Lou, Xirong Guo
BACKGROUND/AIMS: Pro-angiogenic factors VEGF and IL-8 play a major role in modulating the migratory potential of endothelial cells. The goal of this study was to investigate the effect of autocrine VEGF and IL-8 in the form of self-conditioned medium (CM) on human umbilical vein endothelial cells (HUVECs). METHODS: Enzyme-linked immunosorbent assay (ELISA) examined the automatic secretion of VEGF and IL-8 protein by HUVECs. Western blot, small interfering RNA (siRNA), pulldown and Transwell assays were used to explore the role and the mechanism of autocrine VEGF and IL-8 in migration of HUVECs...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28275089/the-microtubule-network-and-cell-death-are-regulated-by-a-mir-34a-stathmin-1-betaiii-tubulin-axis
#19
Nancy S Vetter, E Anders Kolb, Christopher Mills, Valerie B Sampson
MicroRNA-34a (miR-34a) is a master regulator of signaling networks that maintain normal physiology and disease and is currently in development as a miRNA-based therapy for cancer. Prior studies have reported low miR-34a expression in osteosarcoma (OS); however, the molecular mechanisms underlying miR-34a activity in OS are not well defined. Therefore, this study evaluated the role of miR-34a in regulating signal transduction pathways that influence cell death in OS. Levels of miR-34a were attenuated in human OS cells and xenografts of the Pediatric Preclinical Testing Consortium (PPTC)...
March 8, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28263968/snd1-acts-as-a-novel-gene-transcription-activator-recognizing-the-conserved-motif-domains-of-smad-promoters-inducing-tgf%C3%AE-1-response-and-breast-cancer-metastasis
#20
L Yu, Y Di, L Xin, Y Ren, X Liu, X Sun, W Zhang, Z Yao, J Yang
As an AEG-1/MTDH/LYRIC-binding protein, Staphylococcal nuclease domain-containing 1 (SND1) is upregulated in numerous human cancers where it has been assigned multiple functional roles. In this study, we discovered that SND1 was upregulated in breast cancer tissues, particularly the tissues from patients with distant metastases. The underlying molecular mechanisms demonstrated a novel role of SND1 in regulating the activity of transforming growth factor β1 (TGFβ1) signaling pathway, which promotes metastasis in breast cancer...
March 6, 2017: Oncogene
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