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https://www.readbyqxmd.com/read/27934296/identification-of-candidate-host-cell-factors-required-for-actin-based-motility-of-burkholderia-pseudomallei
#1
Niramol Jitprasutwit, Nurhamimah Zainal-Abidin, Charles Vander Broek, Dominic Kurian, Sunee Korbsrisate, Mark P Stevens, Joanne M Stevens
Intracellular actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires the bacterial factor BimA. Located at one pole of the bacterium, BimA recruits and polymerizes cellular actin to promote bacterial motility within and between cells. Here, we describe an affinity approach coupled with mass spectrometry to identify cellular proteins recruited to BimA-expressing bacteria under conditions that promote actin polymerization. We identified a group of cellular proteins that are recruited to the B...
December 2, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27932498/harnessing-molecular-motors-for-nanoscale-pulldown-in-live-cells
#2
Jonathan E Bird, Melanie Barzik, Meghan C Drummond, Daniel C Sutton, Spencer M Goodman, Eva L Morozko, Stacey M Cole, Alexandra K Boukhvalova, Jennifer Skidmore, Diana Syam, Elizabeth A Wilson, Tracy Fitzgerald, Atteeq U Rehman, Donna M Martin, Erich T Boger, Inna A Belyantseva, Thomas B Friedman
Protein-protein interactions (PPIs) regulate assembly of macromolecular complexes, yet these remain challenging to study within the native cytoplasm where they normally exert their biological effect. Here, we miniaturize the concept of affinity pulldown, a gold-standard in vitro PPI interrogation technique, to perform nanoscale pulldowns (NanoSPD) within living cells. NanoSPD hijacks the normal process of intracellular trafficking by myosin motors to forcibly pull fluorescently-tagged protein complexes along filopodial actin filaments...
December 8, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27928058/identification-of-senescence-associated-circular-rnas-sac-rnas-reveals-senescence-suppressor-circpvt1
#3
Amaresh C Panda, Ioannis Grammatikakis, Kyoung Mi Kim, Supriyo De, Jennifer L Martindale, Rachel Munk, Xiaoling Yang, Kotb Abdelmohsen, Myriam Gorospe
Using RNA sequencing (RNA-Seq), we compared the expression patterns of circular RNAs in proliferating (early-passage) and senescent (late-passage) human diploid WI-38 fibroblasts. Among the differentially expressed senescence-associated circRNAs (which we termed 'SAC-RNAs'), we identified CircPVT1, generated by circularization of an exon of the PVT1 gene, as a circular RNA showing markedly reduced levels in senescent fibroblasts. Reducing CircPVT1 levels in proliferating fibroblasts triggered senescence, as determined by a rise in senescence-associated β-galactosidase activity, higher abundance of CDKN1A/P21 and TP53, and reduced cell proliferation...
December 7, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27914499/exercise-training-improves-exercise-capacity-and-quality-of-life-after-transcatheter-aortic-valve-implantation-a-randomized-pilot-trial
#4
Axel Pressler, Jeffrey W Christle, Benjamin Lechner, Viola Grabs, Bernhard Haller, Ina Hettich, David Jochheim, Julinda Mehilli, Rüdiger Lange, Sabine Bleiziffer, Martin Halle
BACKGROUND: Transcatheter aortic valve implantation (TAVI) is increasingly applied for aortic stenosis in elderly patients with impaired mobility and reduced quality of life. These patients may particularly benefit from postinterventional exercise programs, but no randomized study has evaluated the safety and efficacy of exercise in this population. METHODS: In a prospective pilot study, 30 patients after TAVI (mean age, 81±6 years, 44% female, 83±34 days postintervention) were randomly allocated 1:1 to a training group (TG) performing 8 weeks of supervised combined endurance and resistance exercise or to usual care...
December 2016: American Heart Journal
https://www.readbyqxmd.com/read/27893478/loads-and-movement-speeds-dictate-differences-in-power-output-during-circuit-training
#5
Kirk B Roberson, Sean Chowdhari, Morgan White, Joseph F Signorile
Power training has become a common exercise intervention for improving muscle strength, power, and physical function, while reducing injury risk. Few studies, however, have evaluated acute load changes on power output during traditional resistance-training protocols. Therefore, the aim of this study was to quantify the effects of different loading patterns on power output during a single session of circuit resistance training (CRT). Nine males (age=19.4±0.9 y) and eleven females (age=20.6±1.6 y) completed three CRT protocols during separate testing sessions using seven pneumatic exercises...
November 16, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27887846/long-non-coding-rna-metastasis-associated-lung-adenocarcinoma-transcript-1-regulates-the-expression-of-gli2-by-mir-202-to-strengthen-gastric-cancer-progression
#6
Yue Zhang, Zhuo Chen, Ming-Jing Li, Huan-Yu Guo, Nian-Cai Jing
BACKGROUND: Gastric cancer (GC) is one of the most common malignancies and ranks the second leading cause of cancer death worldwide. Some studies had reported the tumor-promoting effects of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) as a competing endogenous RNA (ceRNA) by sponging to microRNAs. However, the molecular mechanism of ceRNA regulatory pathway involving MALAT1 in GC remains unclear. METHODS: MALAT1 and miR -202 expression was detected by quantitative real time PCR (qRT-PCR) in 60 gastric cancer tissues and adjacent normal tissues, CCK8 cell proliferation assays, cell cycle analysis and cell apoptosis assays were performed to detect the GC cell proliferation and apoptosis...
November 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27881679/the-shigella-virulence-factor-icsa-relieves-n-wasp-autoinhibition-by-displacing-the-vca-domain
#7
Rui P M Mauricio, Cy M Jeffries, Dmitri I Svergun, Janet E Deane
Shigella flexneri is a bacterial pathogen that invades cells of the gastrointestinal tract causing severe dysentery. Shigella mediate intracellular motility and spreading via actin comet tail formation. This process is dependent on the surface-exposed, membrane-embedded virulence factor IcsA, which recruits the host actin regulator N-WASP. Although it is clear that Shigella require N-WASP for this process, the molecular details of this interaction and the mechanism of N-WASP activation remain poorly understood...
November 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27863502/annexin-a2-antibodies-but-not-inhibitors-of-the-annexin-a2-heterotetramer-impair-productive-hiv-1-infection-of-macrophages-in-vitro
#8
Andrew W Woodham, Adriana M Sanna, Julia R Taylor, Joseph G Skeate, Diane M Da Silva, Lodewijk V Dekker, W Martin Kast
During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique...
November 18, 2016: Virology Journal
https://www.readbyqxmd.com/read/27849309/activation-of-rac1-and-rhoa-preserve-corneal-endothelial-barrier-function
#9
María C Ortega, Diana Santander-García, Beatriz Marcos-Ramiro, Susana Barroso, Susan Cox, Ignacio Jiménez-Alfaro, Jaime Millán
Purpose: The corneal endothelium is responsible for the correct hydration of the corneal stroma. Corneal endothelial cells have a low proliferative capacity, so preserving their barrier function under suboptimal conditions that cause osmotic imbalance, such as those arising from corneal pathologies, age, cryopreservation, and transplantation, is essential for maintaining corneal transparency. We have investigated the signaling induced by hyperosmotic shock that reversibly disrupts corneal endothelial barriers in human endothelial cells and in murine corneas...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27832197/immunological-and-functional-characterization-of-rhogdi3-and-its-molecular-targets-rhog-and-rhob-in-human-pancreatic-cancerous-and-normal-cells
#10
Mercedes Piedad de León-Bautista, Maria Del Carmen Cardenas-Aguayo, Diana Casique-Aguirre, Manuel Almaraz-Salinas, Sara Parraguirre-Martinez, Angelica Olivo-Diaz, María Del Rocío Thompson-Bonilla, Miguel Vargas
RhoGDI proteins have been implicated in several human cancers; changes in their expression levels have shown pro- or anti-tumorigenic effects. Pancreatic Ductal Adenocarcinoma (PDAC) is a complex pathology, with poor prognosis, and most patients die shortly after diagnosis. Efforts have been focused on understanding the role of RhoGDI's in PDAC, specially, RhoGDI1 and RhoGDI2. However, the role of RhoGDI3 has not been studied in relation to cancer or to PDAC. Here, we characterized the expression and functionality of RhoGDI3 and its target GTPases, RhoG and RhoB in pancreatic cell lines from both normal pancreatic tissue and tissue in late stages of PDAC, and compared them to human biopsies...
2016: PloS One
https://www.readbyqxmd.com/read/27802335/e2f1-and-tfdp1-regulate-pitx1-expression-in-normal-and-osteoarthritic-articular-chondrocytes
#11
Martin Pellicelli, Cynthia Picard, DaShen Wang, Patrick Lavigne, Alain Moreau
We previously reported a loss-of-PITX1 expression in patients suffering of knee/hip osteoarthritis (OA). Search for the mechanism underlying this event led us to discover that PITX1 repression was triggered by the aberrant nuclear accumulation of Prohibitin (PHB1), an E2F1 co-repressor, in OA articular chondrocytes. In the current study, we assessed in details the involvement of E2F transcription factors in regulating PITX1 expression. We also analyzed other genes that are similarly regulated by E2F in regard to osteoarthritis...
2016: PloS One
https://www.readbyqxmd.com/read/27802264/conjugate-selex-a-high-throughput-screening-of-thioaptamer-liposomal-nanoparticle-conjugates-for-targeted-intracellular-delivery-of-anticancer-drugs
#12
Qingshan Mu, Akshaya Annapragada, Mayank Srivastava, Xin Li, Jean Wu, Varatharasa Thiviyanathan, Hongyu Wang, Alexander Williams, David Gorenstein, Ananth Annapragada, Nadarajah Vigneswaran
Patients with advanced head and neck squamous cell carcinoma receiving chemotherapy have a poor prognosis partly due to normal tissue toxicity; therefore, development of a tumor-targeted drug delivery platform to minimize collateral toxicity is a goal of cancer nanomedicine. Aptamers can achieve this purpose. While conventional Systematic Evolution of Ligands by Exponential Enrichment (SELEX) screens aptamer-only libraries and conjugates them to delivery vehicles after selection, we hypothesized that specific delivery requires screening libraries with aptamer-nanoparticle conjugates...
November 1, 2016: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/27768676/sevoflurane-impairs-growth-cone-motility-in-dissociated-murine-neurons
#13
Yun Kyoung Ryu, Reilley P Mathena, Sanghee Lim, Minhye Kwak, Michael Xu, Cyrus D Mintz
BACKGROUND: Early postnatal exposure to general anesthetic agents causes a lasting impairment in learning and memory in animal models. One hypothesis to explain this finding is that exposure to anesthetic agents during critical points in neural development disrupts the formation of brain circuitry. Here, we explore the effects of sevoflurane on the neuronal growth cone, a specialization at the growing end of axons and dendrites that is responsible for the targeted growth that underlies connectivity between neurons...
October 2016: Journal of Neurosurgical Anesthesiology
https://www.readbyqxmd.com/read/27765019/impact-of-rna-degradation-on-fusion-detection-by-rna-seq
#14
Jaime I Davila, Numrah M Fadra, Xiaoke Wang, Amber M McDonald, Asha A Nair, Barbara R Crusan, Xianglin Wu, Joseph H Blommel, Jin Jen, Kandelaria M Rumilla, Robert B Jenkins, Umut Aypar, Eric W Klee, Benjamin R Kipp, Kevin C Halling
BACKGROUND: RNA-seq is a well-established method for studying the transcriptome. Popular methods for library preparation in RNA-seq such as Illumina TruSeq® RNA v2 kit use a poly-A pulldown strategy. Such methods can cause loss of coverage at the 5' end of genes, impacting the ability to detect fusions when used on degraded samples. The goal of this study was to quantify the effects RNA degradation has on fusion detection when using poly-A selected mRNA and to identify the variables involved in this process...
October 20, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27732637/tomosyn-negatively-regulates-arginine-vasopressin-secretion-in-embryonic-stem-cell-derived-neurons
#15
Seiji Takeuchi, Shintaro Iwama, Hiroshi Takagi, Atsushi Kiyota, Kohtaro Nakashima, Hisakazu Izumida, Haruki Fujisawa, Naoko Iwata, Hidetaka Suga, Takashi Watanabe, Kozo Kaibuchi, Yutaka Oiso, Hiroshi Arima, Yoshihisa Sugimura
Arginine vasopressin (AVP) is secreted via exocytosis; however, the precise molecular mechanism underlying the exocytosis of AVP remains to be elucidated. To better understand the mechanisms of AVP secretion, in our study we have identified proteins that bind with a 25 kDa synaptosomal-associated protein (SNAP25). SNAP25 plays a crucial role in exocytosis, in the posterior pituitary. Embryonic stem (ES) cell-derived AVP neurons were established to investigate the functions of the identified proteins. Using glutathione S-transferase (GST)-pulldown assays and proteomic analyses, we identified tomosyn-1 (syntaxin-binding protein 5) as a SNAP25-binding protein in the posterior pituitary...
2016: PloS One
https://www.readbyqxmd.com/read/27708139/regulation-of-gpcr-expression-through-an-interaction-with-cct7-a-subunit-of-the-cct-tric-complex
#16
Samuel Génier, Jade Degrandmaison, Pierrick Moreau, Pascale Labrecque, Terence E Hébert, Jean-Luc Parent
Mechanisms that prevent aggregation and promote folding of nascent G protein-coupled receptors (GPCRs) remain poorly understood. We identified chaperonin containing TCP-1 subunit eta (CCT7) as an interacting partner of the β-isoform of thromboxane A2 receptor (TPβ) by yeast two-hybrid screening. CCT7 coimmunoprecipitated with overexpressed TPβ and β2-adrenergic receptor (β2AR) in HEK 293 cells, but also with endogenous β2AR. CCT7 depletion by small interfering RNA reduced total and cell-surface expression of both receptors and caused redistribution of the receptors to juxtanuclear aggresomes, significantly more so for TPβ than β2AR...
December 1, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27698088/interactions-between-the-cytoplasmic-domains-of-pspb-and-pspc-silence-the-yersinia-enterocolitica-phage-shock-protein-response
#17
Josué Flores-Kim, Andrew J Darwin
: The phage shock protein (Psp) system is a widely conserved cell envelope stress response that is essential for the virulence of some bacteria, including Yersinia enterocolitica Recruitment of PspA by the inner membrane PspB-PspC complex characterizes the activated state of this response. The PspB-PspC complex has been proposed to be a stress-responsive switch, changing from an OFF to an ON state in response to an inducing stimulus. In the OFF state, PspA cannot access its binding site in the C-terminal cytoplasmic domain of PspC (PspC(CT)), because this site is bound to PspB...
December 15, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27672091/identification-of-tension-sensing-motif-of-histone-h3-in-saccharomyces-cerevisiae-and-its-regulation-by-histone-modifying-enzymes
#18
Jianjun Luo, Xiexiong Deng, Christopher Buehl, Xinjing Xu, Min-Hao Kuo
To ensure genome stability during cell division, all chromosomes must attach to spindles emanating from the opposite spindle pole bodies before segregation. The tension between sister chromatids generated by the poleward pulling force is an integral part of chromosome bi-orientation. In budding yeast, the residue Gly44 of histone H3 is critical for retaining the conserved Shugoshin protein Sgo1p at the pericentromeres for monitoring the tension status during mitosis. Studies carried out in this work showed that Lys42, Gly44, and Thr45 of H3 form the core of a tension sensing motif, TSM...
September 26, 2016: Genetics
https://www.readbyqxmd.com/read/27649375/differential-tandem-mass-spectrometry-based-cross-linker-a-new-approach-for-high-confidence-in-identifying-protein-cross-linking
#19
Jayanta K Chakrabarty, Aishwarya G Naik, Michael B Fessler, Gerhard R Munske, Saiful M Chowdhury
Chemical cross-linking and mass spectrometry are now widely used to analyze large-scale protein-protein interactions. The major challenge in cross-linking approaches is the complexity of the mass spectrometric data. New approaches are required that can identify cross-linked peptides with high-confidence and establish a user-friendly analysis protocol for the biomedical scientific community. Here, we introduce a novel cross-linker that can be selectively cleaved in the gas phase using two differential tandem mass-spectrometric fragmentation methods, such as collision-induced or electron transfer dissociation (CID and ETD)...
October 18, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27637083/long-non-coding-rna-crnde-promotes-gallbladder-carcinoma-carcinogenesis-and-as-a-scaffold-of-dmbt1-and-c-iap1-complexes-to-activating-pi3k-akt-pathway
#20
Sheng Shen, Han Liu, Yueqi Wang, Jiwen Wang, Xiaolin Ni, Zhilong Ai, Hongtao Pan, Houbao Liu, Yebo Shao
Deleted in malignant brain tumors 1 (DMBT1) is deleted during cancer progression and as a potential tumor-suppressor gene in various types of cancer. However, its role in Gallbladder cancer remains poorly understood. DMBT1 has low-expression and deletion of copy number were detected in normal tissues and GBC cancer tissues by qRT-PCR. Knockdown of DMBT1 increased migration and invasion and overexpressed DMBT1 impaired migration and invasion in GBC cells. We also evaluated the molecular mechanism of DMBT1 by RNA sequencing and GSEA analysis...
September 14, 2016: Oncotarget
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