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https://www.readbyqxmd.com/read/28719045/mical2-promotes-breast-cancer-cell-migration-by-maintaining-egfr-stability-and-egfr-p38-signaling-activation
#1
Yueyuan Wang, Wenjie Deng, Yujie Zhang, Shixiu Sun, Shuo Zhao, Yan Chen, Xuyang Zhao, Lei Liu, Jun Du
AIM: MICAL2, a cytoskeleton dynamics regulator, is identified associated with survival and metastasis of several types of cancers recently. The present study was designed to investigate the role of MICAL2 in breast cancer cell migration as well as its underlying mechanisms. METHODS: The relationship between MICAL2 and EGF/EGFR signaling was analyzed by using gene overexpression and knockdown techniques. Cell migration was measured by wound healing assays. Activation of EGF/EGFR singaling pathways were evaluated by immunofluorescence, qPCR, Western blotting and zymography techniques...
July 18, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28704058/elucidating-protein-dna-interactions-in-human-alphoid-chromatin-via-hybridization-capture-and-mass-spectrometry
#2
Katherine E Buxton, Julia Kennedy-Darling, Michael R Shortreed, Nur Zafirah Zaidan, Michael Olivier, Mark Scalf, Rupa Sridharan, Lloyd M Smith
The centromere is the chromosomal locus where the kinetochore forms, and is critical for ensuring proper segregation of sister chromatids during cell division. A substantial amount of effort has been devoted to understanding the characteristic features and roles of the centromere, yet some fundamental aspects of the centromere, such as the complete list of elements that define it, remain obscure. It is well known that human centromeres include a highly repetitive class of DNA known as alpha satellite, or alphoid, DNA...
July 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28701209/alantolactone-a-natural-sesquiterpene-lactone-has-potent-antitumor-activity-against-glioblastoma-by-targeting-ikk%C3%AE-kinase-activity-and-interrupting-nf-%C3%AE%C2%BAb-cox-2-mediated-signaling-cascades
#3
Xun Wang, Zhenlong Yu, Chao Wang, Wei Cheng, Xiangge Tian, Xiaokui Huo, Yan Wang, Chengpeng Sun, Lei Feng, Jinshan Xing, Yulong Lan, Dongdong Sun, Qingjuan Hou, Baojing Zhang, Xiaochi Ma, Bo Zhang
BACKGROUND: Glioblastoma multiforme (GBM) is one of the most refractory and palindromic central nervous system (CNS) neoplasms, and current treatments have poor effects in GBM patients. Hence, the identification of novel therapeutic targets and the development of effective treatment strategies are essential. Alantolactone (ATL) has a wide range of pharmacological activities, and its anti-tumor effect is receiving increasing attention. However, the molecular mechanism underlying the anti-GBM activity of ATL remains poorly understood...
July 12, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28699205/native-kinesin-1-does-not-bind-preferentially-to-gtp-tubulin-rich-microtubules-in-vitro
#4
Qiaochu Li, Stephen J King, Jing Xu
Molecular motors such as kinesin-1 work in small teams to actively shuttle cargos in cells, for example in polarized transport in axons. Here we examined the potential regulatory role of the nucleotide state of tubulin on the run length of cargos carried by multiple kinesin motors, using an optical trapping-based in vitro assay. Based on a previous report that kinesin binds preferentially to GTP-tubulin-rich microtubules, we anticipated that multiple-kinesin cargos would run substantially greater distances along GMPCPP microtubules than along GDP microtubules...
July 12, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28683446/lncrna-snhg6-is-associated-with-poor-prognosis-of-gastric-cancer-and-promotes-cell-proliferation-and-emt-through-epigenetically-silencing-p27-and-sponging-mir-101-3p
#5
Kai Yan, Jie Tian, Wenzheng Shi, Hao Xia, Yuanfang Zhu
Background/Amis: Long non-coding RNAs (lncRNAs), a novel class of transcripts, have been shown to play critical roles in diverse cellular biological processes, including tumorigenesis. Small nucleolar RNA host gene 6 (SNHG6) regulates various biological processes in cancer cells. However, the biological role of SNHG6 in gastric cancer still remains to be explored. The aim of this study is to investigate the characteristic of the SNHG6 in gastric cancer. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of SNHG6 in gastric cancer tissues and cell lines...
June 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28657147/lncrna-pvt1-promotes-pancreatic-cancer-cells-proliferation-and-migration-through-acting-as-a-molecular-sponge-to-regulate-mir-448
#6
Liang Zhao, Hongru Kong, Hongwei Sun, Zongjing Chen, Bicheng Chen, Mengtao Zhou
The identification and characterization of long non-coding RNAs (lncRNAs) in diverse biological process has currently developed rapidly. LncRNA-PVT1, located adjacent to the MYC locus on chromosomal region 8q24, has been reported to be associated with many biological processes. However, the function and mechanism of PVT1 in pancreatic carcinoma (PC) is poorly understood. In this present study, we first measured the level of PVT1 in the PC cell lines and tissues by quantitative real-time PCR (qRT-PCR), and then employed loss-of-function and gain-of-function approaches to explore the association between PVT1 expression levels and PC cell proliferation/migration ability...
June 28, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28636190/uchl1-promotes-invasion-of-breast-cancer-cells-through-activating-akt-signaling-pathway
#7
Yanhong Luo, Jianfeng He, Chunlin Yang, Matthew Orange, Xingcong Ren, Nick Blair, Tao Tan, Jin-Ming Yang, Hua Zhu
As a de-ubiquitin enzyme, ubiquitin C-terminal hydrolase (UCH)-L1 has been shown to be overexpressed in several human cancers. However, the function of UCH-L1 in invasion of breast cancers is still unclear. Here we report that the expression of UCH-L1 is significantly higher in cancer cells with higher invasive ability. While ectopic UCH-L1 expression failed to alter cell proliferation in MCF-7 cells, it caused a significant upregulation of cellular invasion. Furthermore, siRNA mediated knockdown of UCH-L1 led to suppression of invasion in UCH-L1 overexpressing MCF-7 cells...
June 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28628370/multiple-joint-exercises-using-elastic-resistance-bands-vs-conventional-resistance-training-equipment-a-cross-over-study
#8
Vegard M Iversen, Paul Jarle Mork, Ottar Vasseljen, Ronny Bergquist, Marius S Fimland
Previous studies indicate that elastic resistance bands (ERB) can be a viable option to conventional resistance-training equipment (CRE) during single-joint resistance exercises, but their efficacy has not been established for several commonly used multiple-joint resistance exercises. Thus, we compared muscular activation levels in four popular multiple-joint exercises performed with ERB (TheraBand(®)) vs. CRE (Olympic barbell or cable pulley machines). In a cross-over design, men and women (n = 29) performed squats, stiff-legged deadlifts, unilateral rows and lateral pulldown using both modalities...
June 19, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28625921/binding-of-coronin-1b-to-t%C3%AE-ri-negatively-regulates-the-tgf%C3%AE-1-signaling-pathway
#9
Geun-Young Kim, Hyun-Joung Lim, Hyun-Young Park
Coronin 1B is an actin-binding protein that regulates several actin-dependent cellular processes including migration and endocytosis. However, the role of coronin 1B in the tumor growth factor (TGF)β signaling pathway is largely unknown. Here, we investigated whether coronin 1B affects the TGFβ signaling cascade and found that coronin 1B negatively regulates the TGFβ signaling pathway. Immunoprecipitation and glutathione-S-transferase-pulldown assays revealed that coronin 1B directly associated with TGFβ receptor I (TβRI)...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28607052/efficient-ultra-high-affinity-chromatography-in-a-one-step-purification-of-complex-proteins
#10
Marina N Vassylyeva, Sergiy Klyuyev, Alexey D Vassylyev, Hunter Wesson, Zhuo Zhang, Matthew B Renfrow, Hengbin Wang, N Patrick Higgins, Louise T Chow, Dmitry G Vassylyev
Protein purification is an essential primary step in numerous biological studies. It is particularly significant for the rapidly emerging high-throughput fields, such as proteomics, interactomics, and drug discovery. Moreover, purifications for structural and industrial applications should meet the requirement of high yield, high purity, and high activity (HHH). It is, therefore, highly desirable to have an efficient purification system with a potential to meet the HHH benchmark in a single step. Here, we report a chromatographic technology based on the ultra-high-affinity (Kd ∼ 10(-14)-10(-17) M) complex between the Colicin E7 DNase (CE7) and its inhibitor, Immunity protein 7 (Im7)...
June 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28592633/the-phospho-caveolin-1-scaffolding-domain-dampens-force-fluctuations-in-focal-adhesions-and-promotes-cancer-cell-migration
#11
Fanrui Meng, Sandeep Saxena, Youtao Liu, Bharat Joshi, Timothy H Wong, Jay Shankar, Leonard J Foster, Pascal Bernatchez, Ivan R Nabi
Caveolin-1 (Cav1), a major Src kinase substrate phosphorylated on tyrosine-14 (Y14), contains the highly-conserved membrane-proximal caveolin scaffolding domain (CSD; amino acids 82-101). Here we show, using CSD mutants (F92A/V94A) and membrane-permeable CSD competing peptides, that Src kinase-dependent pY14Cav1 regulation of focal adhesion protein stabilization, focal adhesion tension and cancer cell migration is CSD-dependent. Quantitative proteomic analysis of Cav1-GST (1-101 amino acids) pulldowns showed 6-fold increased binding of vinculin and, to a lesser extent, α-actinin, talin and filamin, to phosphomimetic Cav1Y14D relative to non-phosphorylatable Cav1Y14F...
June 7, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28584154/pa3225-is-a-transcriptional-repressor-of-antibiotic-resistance-mechanisms-in-pseudomonas-aeruginosa
#12
Clayton W Hall, Li Zhang, Thien-Fah Mah
The tssABC1 locus is part of the Hcp secretion island (HSI) -I type VI secretion system (T6SS) in Pseudomonas aeruginosa Previous work has implicated the tssC1 gene in P. aeruginosa biofilm-specific antibiotic resistance, and tssC1 is preferentially expressed in biofilms compared to planktonic cells. Using a DNA-dependent protein pulldown approach, we discovered PA3225, an uncharacterised LysR-type transcriptional regulator that specifically bound to the tssABC1 upstream regulatory region. Deletion of PA3225 led to a two-fold decrease in tssA1 expression in planktonic cells compared to wild-type, and tssA1 expression was slightly reduced in ΔPA3225 biofilms compared to wild-type biofilms...
June 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28580901/prosurvival-long-noncoding-rna-pincr-regulates-a-subset-of-p53-targets-in-human-colorectal-cancer-cells-by-binding-to-matrin-3
#13
Ritu Chaudhary, Berkley Gryder, Wendy S Woods, Murugan Subramanian, Matthew F Jones, Xiao Ling Li, Lisa M Jenkins, Svetlana A Shabalina, Min Mo, Mary Dasso, Yuan Yang, Lalage M Wakefield, Yuelin Zhu, Susan M Frier, Branden S Moriarity, Kannanganattu V Prasanth, Pablo Perez-Pinera, Ashish Lal
Thousands of long noncoding RNAs (lncRNAs) have been discovered, yet the function of the vast majority remains unclear. Here, we show that a p53-regulated lncRNA which we named PINCR (p53-induced noncoding RNA), is induced ~100-fold after DNA damage and exerts a prosurvival function in human colorectal cancer cells (CRC) in vitro and tumor growth in vivo. Targeted deletion of PINCR in CRC cells significantly impaired G1 arrest and induced hypersensitivity to chemotherapeutic drugs. PINCR regulates the induction of a subset of p53 targets involved in G1 arrest and apoptosis, including BTG2, RRM2B and GPX1...
June 5, 2017: ELife
https://www.readbyqxmd.com/read/28566286/rab35-protein-regulates-evoked-exocytosis-of-endothelial-weibel-palade-bodies
#14
Anja Biesemann, Alexandra Gorontzi, Francis Barr, Volker Gerke
Weibel-Palade bodies (WPB) are secretory organelles of endothelial cells that undergo evoked exocytosis following intracellular Ca(2+) or cAMP elevation, thereby supplying the vasculature with factors controlling hemostasis. Several cytosolic and membrane-associated proteins, including the Rab family members Rab3, Rab15, and Rab27a, have been implicated in regulating the acute exocytosis of WPB. Here, we carried out a genome-wide screen to identify Rab pathways affecting WPB exocytosis. Overexpression of a specific subset of Rab GTPase-activating proteins (RabGAPs) inhibited histamine-evoked, Ca(2+)-dependent WPB exocytosis, presumably by inactivating the target Rab GTPases...
July 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28557180/targeting-g-protein-coupled-receptors-by-capture-compound-mass-spectrometry-ccms-a-case-study-with-sertindole
#15
Christian Blex, Simon Michaelis, Anna K Schrey, Jens Furkert, Jenny Eichhorst, Kathrin Bartho, Frederick G Quast, Anett Marais, Matthias Hakelberg, Uschi Gruber, Sylvia Niquet, Oliver Popp, Friedrich Kroll, Michael Sefkow, Ralf Schülein, Dreger Mathias, Hubert Köster
Unbiased chemoproteomic profiling of small molecule interactions with endogenous proteins is important for drug discovery. For meaningful results, all protein classes have to be tractable, including G-protein coupled receptors (GPCRs). These are hardly tractable by affinity pulldown from lysates. We report a Capture Compound (CC)-based strategy to target and identify GPCRs directly from living cells. We synthesized CCs with sertindole attached to the CC scaffold in different orientations to target the dopamine D2 receptor (DRD2) heterologously expressed in HEK293 cells...
May 29, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28539358/protein-kinase-c-%C3%AE%C2%B5-stabilizes-%C3%AE-catenin-and-regulates-its-subcellular-localization-in-podocytes
#16
Michelle Duong, Xuejiao Yu, Beina Teng, Patricia Schroder, Hermann Haller, Susanne Eschenburg, Mario Schiffer
Kidney disease has been linked to dysregulated signaling via protein kinase C (PKC) in kidney cells such as podocytes. PKCα is a conventional isoform of PKC and a well-known binding partner of β-catenin, which promotes its degradation. β-Catenin is the main effector of the canonical Wnt pathway and is critical in cell adhesion. However, whether other PKC isoforms interact with β-catenin has not been studied systematically. Here we demonstrate that PKCε-deficient mice, which develop proteinuria and glomerulosclerosis, display a lower β-catenin expression compared to PKC wildtype mice, consistent with an altered phenotype of podocytes in culture...
May 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28523344/role-of-g3bp1-in-glucocorticoid-receptor-mediated-microrna-15b-and-microrna-23a-biogenesis-in-endothelial-cells
#17
Hoi-Hin Kwok, Po-Ying Poon, Kylie Hin-Man Mak, Lin-Yao Zhang, Pei Liu, Huoming Zhang, Nai-Ki Mak, Patrick Ying-Kit Yue, Ricky Ngok-Shun Wong
MicroRNAs (miRNAs) are a family of non-coding RNAs that play crucial roles in regulating various normal cellular responses. Recent studies revealed that the canonical miRNA biogenesis pathway is subject to sophisticated regulation. Hormonal control of miRNA biogenesis by androgen and estrogen has been demonstrated, but the direct effects of the glucocorticoid receptor (GR) on miRNA biogenesis are unknown. This study revealed the role of GR in miRNA maturation. We showed that two GR agonists, dexamethasone and ginsenoside-Rg1 rapidly suppressed the expression of mature miR-15b, miR-23a, and miR-214 in human endothelial cells...
May 18, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28516119/affinity-pulldown-of-biotinylated-rna-for-detection-of-protein-rna-complexes
#18
Amaresh C Panda, Jennifer L Martindale, Myriam Gorospe
RNA-binding proteins (RBPs) have recently emerged as crucial players in the regulation of gene expression. The interactions of RBPs with target mRNAs control the levels of gene products by altering different regulatory steps, including pre-mRNA splicing and maturation, nuclear mRNA export, and mRNA stability and translation (Glisovic et al., 2008). There are several methodologies available today to identify RNAs bound to specific RBPs; some detect only recombinant molecules in vitro, others detect recombinant and endogenous molecules, while others detect only endogenous molecules...
December 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28502712/ligation-mediated-pcr-with-a-back-to-back-adapter-reduces-amplification-bias-resulting-from-variations-in-gc-content
#19
Satoru Ishihara, Naoe Kotomura, Naoki Yamamoto, Hiroshi Ochiai
Ligation-mediated polymerase chain reaction (LM-PCR) is a common technique for amplification of a pool of DNA fragments. Here, a double-stranded oligonucleotide consisting of two primer sequences in back-to-back orientation was designed as an adapter for LM-PCR. When DNA fragments were ligated with this adapter, the fragments were sandwiched between two adapters in random orientations. In the ensuing PCR, ligation products linked at each end to an opposite side of the adapter, i.e. to a distinct primer sequence, were preferentially amplified compared with products linked at each end to an identical primer sequence...
August 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28474873/circuit-strength-training-improves-muscle-strength-functional-performance-and-anthropometric-indicators-in-sedentary-elderly-women
#20
Mauro L Mazini Filho, Felipe J Aidar, Dihogo Gama de Matos, Osvaldo Costa Moreira, Cláudia E Patrocínio de Oliveira, Gabriela R de Oliveira Venturini, Victor Magalhães Curty, Henrique Menezes Touguinha, Maria E Caputo Ferreira
BACKGROUND: This study aimed to investigate the effects of circuit strength training on the muscle strength, functional autonomy and anthropometric indicators of the elderly. METHODS: Were included 65 women divided in two groups: strength training (TG, n= 34) and control group (CG, n = 31). The strength-training group was subjected to a circuit shaped training program, three days per week, for a period of 12 weeks. In each training session, the circuit was repeated three times...
April 26, 2017: Journal of Sports Medicine and Physical Fitness
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