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Circulating tumor cells

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https://www.readbyqxmd.com/read/29690766/co-delivery-of-doxorubicin-and-anti-bcl-2-sirna-by-ph-responsive-polymeric-vector-to-overcome-drug-resistance-in-in-vitro-and-in-vivo-hepg2-hepatoma-model
#1
Weitong Sun, Xiaoyan Chen, Chao Xie, Yong Wang, Liteng Lin, Kangshun Zhu, Xintao Shuai
Drug resistance, developed through multiple mechanisms, is a major hindrance to successful chemotherapy of tumor. Combination therapy of chemotherapeutic drugs and siRNA represents an emerging strategy which may improve anticancer effect by synergistic actions. In this study, triblock copolymer of poly(ethylene glycol)-block-poly(L-lysine)-block-poly aspartyl (N-(N',N'-diisopropylaminoethyl)) (PEG-PLL-PAsp(DIP)) was synthesized for the first time to enable the codelivery of BCL-2 siRNA and DOX. The system is supposed to only bypass drug efflux but also down-regulate the anti-apoptotic gene and consequently confronting against chemoresistance as well...
April 24, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29689598/correlation-between-circulating-cell-free-pik3ca-tumor-dna-levels-and-treatment-response-in-patients-with-pik3ca-mutated-metastatic-breast-cancer
#2
Annette R Kodahl, Sidse Ehmsen, Niels Pallisgaard, Anne Marie B Jylling, Jeanette Dupont Jensen, Anne-Vibeke Laenkholm, Ann S Knoop, Henrik J Ditzel
Liquid biopsies focusing on the analysis of circulating cell-free tumor DNA (ctDNA) may have important clinical implications for personalized medicine, including early detection of cancer, therapeutic guidance and monitoring of recurrence. Mutations in the oncogene, PIK3CA, are frequently observed in breast cancer and have been suggested as a predictive biomarker for PI3K-selective inhibitor treatment. In this study, we analyzed the presence of PIK3CA mutations in formalin-fixed, paraffin-embedded, metastatic tissue and corresponding ctDNA from serum of patients with advanced breast cancer using a highly-sensitive, optimized droplet digital PCR (ddPCR) assay...
April 24, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29689288/self-assembled-amphiphilic-zein-lactoferrin-micelles-for-tumor-targeted-co-delivery-of-rapamycin-and-wogonin-to-breast-cancer
#3
Sally A Sabra, Ahmed O Elzoghby, Salah A Sheweita, Medhat Haroun, Maged W Helmy, Maha A Eldemellawy, Ying Xia, David Goodale, Alison L Allan, Sohrab Rohani
Protein-based micelles have shown significant potential for tumor-targeted delivery of anti-cancer drugs. In this light, self-assembled nanocarriers based on GRAS (Generally recognized as safe) amphiphilic protein co-polymers were synthesized via carbodiimide coupling reaction. The new nano-platform is composed of the following key components: i) hydrophobic zein core to encapsulate the hydrophobic drugs rapamycin (RAP) and wogonin (WOG) with high encapsulation efficiency, ii) hydrophilic lactoferrin (Lf) corona to enhance the tumor targeting, and prolong systemic circulation of the nanocarriers, and iii) glutaraldehyde (GLA)-crosslinking to reduce the particle size and improve micellar stability...
April 21, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29687965/developments-in-label-free-microfluidic-methods-for-single-cell-analysis-and-sorting
#4
REVIEW
Thomas R Carey, Kristen L Cotner, Brian Li, Lydia L Sohn
Advancements in microfluidic technologies have led to the development of many new tools for both the characterization and sorting of single cells without the need for exogenous labels. Label-free microfluidics reduce the preparation time, reagents needed, and cost of conventional methods based on fluorescent or magnetic labels. Furthermore, these devices enable analysis of cell properties such as mechanical phenotype and dielectric parameters that cannot be characterized with traditional labels. Some of the most promising technologies for current and future development toward label-free, single-cell analysis and sorting include electronic sensors such as Coulter counters and electrical impedance cytometry; deformation analysis using optical traps and deformation cytometry; hydrodynamic sorting such as deterministic lateral displacement, inertial focusing, and microvortex trapping; and acoustic sorting using traveling or standing surface acoustic waves...
April 24, 2018: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
https://www.readbyqxmd.com/read/29686738/a-new-approach-to-epigenome-wide-discovery-of-non-invasive-methylation-biomarkers-for-colorectal-cancer-screening-in-circulating-cell-free-dna-using-pooled-samples
#5
María Gallardo-Gómez, Sebastian Moran, María Páez de la Cadena, Vicenta Soledad Martínez-Zorzano, Francisco Javier Rodríguez-Berrocal, Mar Rodríguez-Girondo, Manel Esteller, Joaquín Cubiella, Luis Bujanda, Antoni Castells, Francesc Balaguer, Rodrigo Jover, Loretta De Chiara
Background: Colorectal cancer is the fourth cause of cancer-related deaths worldwide, though detection at early stages associates with good prognosis. Thus, there is a clear demand for novel non-invasive tests for the early detection of colorectal cancer and premalignant advanced adenomas, to be used in population-wide screening programs. Aberrant DNA methylation detected in liquid biopsies, such as serum circulating cell-free DNA (cfDNA), is a promising source of non-invasive biomarkers...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29685627/arginine-pretreatment-enhances-circulating-endothelial-progenitor-cell-population-and-attenuates-inflammatory-response-in-high-fat-diet-induced-obese-mice-with-limb-ischemia
#6
Yu-Fan Kuo, Chiu-Li Yeh, Juey-Ming Shih, Yao-Ming Shih, Sung-Ling Yeh
Obesity is a global health problem with an up-regulated inflammatory reaction. Obesity-induced endothelial progenitor cells (EPCs) dysfunction is associated with vascular complications that may contribute to critical limb ischemia. Arginine (Arg) is an amino acid with immune-modulatory property and has been found to promote EPCs mobilization in disease conditions. Thus in the present investigation, we hypothesized that arginine given to a murine model of diet-induced obesity would increase circulating EPCs and mitigate the inflammatory reactions in response to limb ischemia...
March 23, 2018: Nutrition Research
https://www.readbyqxmd.com/read/29684573/bevacizumab-reduces-s100a9-mdsc-linked-to-intracranial-control-in-patients-with-egfr-mutant-lung-adenocarcinoma
#7
Po-Hao Feng, Kuan-Yuan Chen, Yu-Chen Huang, Ching-Shan Luo, Shen Ming Wu, Tzu-Tao Chen, Chun-Nin Lee, Chi-Tai Yeh, Hsiao-Chi Chuang, Chia-Li Han, Chiou-Feng Lin, Wei-Hwa Lee, Chih-Hsi Kuo, Kang-Yun Lee
BACKGROUND: In vitro models have demonstrated immune-modulating effects of bevacizumab. Combinations of EGFR tyrosine kinase inhibitor (TKI) with bevacizumab improve progression free survival (PFS) in patients with EGFR mutated lung adenocarcinoma. How bevacizumab confers this clinical effect and underlying mechanisms are not clear. PATIENT AND METHODS: Fifty-five patients with stage 4 EGFR mutated lung adenocarcinoma were enrolled. Myeloid derived suppressor cells (MDSCs), T helper cells (Th1/Th2) and cytotoxic T lymphocytes (CTLs) were analyzed by flow cytometry...
April 20, 2018: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/29683844/circulating-inflammatory-monocytes-contribute-to-impaired-influenza-vaccine-responses-in-hiv-infected-participants
#8
Varghese K George, Suresh Pallikkuth, Rajendra Pahwa, Lesley de Armas, Stefano Rinaldi, Li Pan, Savita Pahwa
OBJECTIVE: Antibody responses are often impaired in old age and in HIV+ infection despite virologic control with antiretroviral therapy but innate immunologic determinants are not well understood. DESIGN: Monocytes and natural killer cells were examined for relationships to age, HIV infection and influenza vaccine responses. METHODS: Virologically suppressed HIV+ (n = 139) and HIV- (n = 137) participants classified by age as young (18-39 years), middle-aged (40-59 years) and old (≥60 years) were evaluated preinfluenza and postinfluenza vaccination...
April 19, 2018: AIDS
https://www.readbyqxmd.com/read/29683761/circulating-micrornas-associated-with-prolonged-overall-survival-in-lung-cancer-patients-treated-with-nivolumab
#9
Ann Rita Halvorsen, Vandana Sandhu, Mette Sprauten, Vidar G Flote, Elin H Kure, Odd Terje Brustugun, Åslaug Helland
BACKGROUND: The introduction of immune check-point inhibition in non-small cell lung cancer (NSCLC) therapy represents improved prospects for the patients. The response rates to check-point inhibitors are approximately 20% in unselected NSCLC patients. Increasing levels of tumor PD-L1 expression are associated with higher response rates. However, patients with low PD-L1 levels may also have durable responses, and improved strategies for patient stratification are needed. MATERIAL AND METHODS: In this study, we investigated circulating microRNAs aiming to identify circulating predictive biomarkers associated with increased overall survival after immune check-point treatment...
April 23, 2018: Acta Oncologica
https://www.readbyqxmd.com/read/29683641/efficient-capture-and-high-activity-release-of-circulating-tumor-cells-by-using-tio2-nanorod-arrays-coated-with-soluble-mno2-nanoparticles
#10
Rui Li, Fangfang Chen, Huiqin Liu, Zixiang Wang, Zitong Zhang, Yuan Wang, Heng Cui, Wei Liu, Xingzhong Zhao, Zhi-Jun Sun, Shi-Shang Guo
Effective capture and release of circulating tumor cells(CTCs) with high viability is still a challenge in medical research. We design a novel approach with efficient yield, high cell activity to capture and release of CTCs. Our platform is based on TiO2 nanorod arrays coated with transparent MnO2 nanoparticles. We use hydrothermal synthesis to prepare TiO2 nanorod arrays, the MnO2 nanoparticles are fabricated through in situ self-assembly on the substrate to form a monolayer and etched by oxalic acid with low concentration at room temperature...
April 23, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29683554/monitoring-circulating-prostate-cancer-cells-by-in-vivo-flow-cytometry-assesses-androgen-deprivation-therapy-on-metastasis
#11
Kai Pang, Chengying Xie, Zhangru Yang, Yuanzhen Suo, Xi Zhu, Dan Wei, Xiaofu Weng, Xunbin Wei, Zhengqin Gu
It remains controversial whether surgical castration prolongs survival rate and improves therapy prospects in patients suffering from prostate cancer. We used PC3 cell line to establish prostate tumor models. In vivo flow cytometry and ultrasonic imaging were used to monitor the process of prostate cancer growth, development and metastasis. We found out that the number of circulating tumor cells (CTCs) in orthotopic tumor model was higher than that in subcutaneous tumor model. The CTC number in orthotopic tumor model was due to burst growth, while CTC number in subcutaneous tumor model showed a gradual increase with tumor size...
April 23, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29683453/a-blood-based-test-for-the-detection-of-ros1-and-ret-fusion-transcripts-from-circulating-ribonucleic-acid-using-digital-polymerase-chain-reaction
#12
Hestia S Mellert, Kristin E Alexander, Leisa P Jackson, Gary A Pestano
We have developed novel methods for the isolation and characterization of tumor-derived circulating ribonucleic acid (cRNA) for blood-based liquid biopsy. Robust detection of cRNA recovered from blood represents a solution to a critical unmet need in clinical diagnostics. The test begins with the collection of whole blood into blood collection tubes containing preservatives that stabilize cRNA. Cell-free, exosomal, and platelet-associated RNA is isolated from plasma in this test system. The cRNA is reverse transcribed to complementary DNA (cDNA) and amplified using digital polymerase chain reaction (dPCR)...
April 5, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29683140/liquid-biopsy-in-the-omics-era-of-tumor-medicine
#13
Yi Jiang, Denong Wang
Liquid biopsy uses noninvasive blood test to assess tumor heterogeneity and evolution in real time. It looks for tumor components in the blood circulation, such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA), to provide tumor-specific information. By detecting multiplex tumor biomarkers, including nucleic acids, proteins, carbohydrates, and other tumor-derived substances, liquid biopsy helps with early tumor diagnosis, tumor evolution monitoring, and prognosis prediction. With the development of high-throughput OMICS tools like carbohydrate microarray and high-speed fiber-optic array scanning technology (FAST scan), it is now practical to identify glycan markers of CTCs and cancer stem cells (CSCs), especially those that are cell-surface exposed and readily accessible for immune recognition and targeting...
2018: Open access journal of biomedical engineering and its applications
https://www.readbyqxmd.com/read/29682444/molecular-detection-of-emt-markers-in-circulating-tumor-cells-from-metastatic-non-small-cell-lung-cancer-patients-potential-role-in-clinical-practice
#14
Annalisa Milano, Francesca Mazzetta, Sabatino Valente, Danilo Ranieri, Laura Leone, Andrea Botticelli, Concetta Elisa Onesti, Salvatore Lauro, Salvatore Raffa, Maria Rosaria Torrisi, Paolo Marchetti
Background: Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related mortality; nevertheless, there are few data regarding detection of circulating tumor cells (CTCs) in NSCLC, compared to other kinds of cancers in which their prognostic roles have already been defined. This difference is likely due to detection methods based on the epithelial marker expression which ignore CTCs undergoing epithelial-mesenchymal transition (CTCsEMT ). Methods: After optimization of the test with spiking experiments of A549 cells undergoing TGF- β 1-induced EMT (A549EMT ), the CTCsEMT were enriched by immunomagnetic depletion of leukocytes and then characterized by a RT-PCR assay based on the retrieval of epithelial and EMT-related genes...
2018: Analytical Cellular Pathology (Amsterdam)
https://www.readbyqxmd.com/read/29679605/circulating-cell-free-dna-in-patients-with-alveolar-echinococcosis
#15
Alice Baraquin, Eric Hervouet, Carine Richou, Pierre Flori, Paul Peixoto, Amel Azizi, Eric Delabrousse, Oleg Blagosklonov, Gerald Umhang, Solange Bresson-Hadni, Benoit Valot, Frédéric Grenouillet
Alveolar echinococcosis (AE) is a parasitic disease, due to Echinococcus multilocularis. Often compared to liver cancer, it develops by infiltration from its primary site to the surrounding tissue, and can then metastasize to other organs. Detection of circulating cell-free DNA (ccfDNA) is a useful analytical tool in oncology, for diagnosis, prognosis, and therapy monitoring. This study sought to investigate the presence of ccfDNA in patients with AE, and its potential usefulness for the evaluation of treatment efficiency...
April 18, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29679570/hmgb1-containing-nucleosome-mediates-chemotherapy-induced-metastasis-of-human-lung-cancer
#16
Kailing Wang, Shan Shan, Shenqi Wang, Xiaohua Gu, Xiaohui Zhou, Tao Ren
While chemotherapy is an important and widely used therapeutic for cancer, it may facilitate cancer metastasis. Herein, we report that human lung cancer cells exerted higher invasion and metastasis after chemotherapy. In a human lung cancer xenograft model, chemotherapy promotes the cancer invasion and metastasis in HMGB1-dependent manner. Further studies identify HMGB1-containing nucleosome from chemotherapy-induced apoptotic cancer cells as an effective factor. Such nucleosome functions through TLR4 and TLR9 to drive cancer invasion and metastasis...
April 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29676924/paclitaxel-loaded-ph-sensitive-liposome-new-insights-on-structural-and-physicochemical-characterization
#17
Liziane Oliveira Fonseca Monteiro, Angelo Malachias, Gwenaelle Pound-Lana, Rogerio Magalhães-Paniago, Vanessa Carla Furtado Mosqueira, Mônica Cristina Oliveira, Andre Branco de Barros, Elaine Amaral Leite
A long-circulating and pH-sensitive liposome containing PTX (SpHL-PTX) was recently developed by our group. Once at an acidic environment, e.g. tumor, these liposomes undergo destabilization releasing the encapsulated drug. In this way, the aim of this study was evaluate the molecular and supramolecular interactions between the lipid bilayer and PTX in similar biological environment conditions. High sensitivity analyses of SpHL-PTX structures were obtained by small angle X-ray scattering (SAXS) technique combined with other techniques such as DLS, AF4, TEM and HPLC...
April 20, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29676587/a-potential-role-for-exosomal-tctp-export-in-vascular-remodeling-in-pulmonary-arterial-hypertension
#18
Elisabet Ferrer, Benjamin J Dunmore, Dhiya Hassan, Mark L Ormiston, Stephen Moore, John Deighton, Lu Long, Xu Dong Yang, Duncan J Stewart, Nicholas W Morrell
Pulmonary arterial hypertension (PAH) is characterized by increased proliferation and resistance to apoptosis of pulmonary vascular cells. Increased expression of translationally controlled tumor protein (TCTP), a pro-survival and anti-apoptotic mediator, has recently been demonstrated in patients with hereditary PAH (HPAH) although its role in the pathobiology of PAH remains unclear. Silencing of TCTP in blood outgrowth endothelial cells (BOECs) isolated from control subjects led to significant changes in morphology, cytoskeletal organization, increased apoptosis and decreased directionality during migration...
April 20, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29675023/increased-plasma-levels-of-danger-associated-molecular-patterns-are-associated-with-immune-suppression-and-postoperative-infections-in-patients-undergoing-cytoreductive-surgery-and-hyperthermic-intraperitoneal-chemotherapy
#19
Guus P Leijte, Hettie Custers, Jelle Gerretsen, Amon Heijne, Johannes Roth, Thomas Vogl, Gert J Scheffer, Peter Pickkers, Matthijs Kox
Introduction: Danger-associated molecular patterns (DAMPs) can elicit immune responses and may subsequently induce an immune-suppressed state. Previous work showed that increased plasma levels of DAMPs are associated with immune suppression and increased susceptibility toward infections in trauma patients. Like trauma, major surgical procedures, such as cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC), are also thought to cause profound DAMP release...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29674255/topically-applied-azaphenothiazines-inhibit-experimental-psoriasis-in-mice
#20
Jolanta Artym, Maja Kocięba, Ewa Zaczyńska, Iwona Kochanowska, Michał Zimecki, Wojciech Kałas, Anna Fiedorowicz, Alicja Pawlak, Leon Strządała, Małgorzata Jeleń, Beata Morak-Młodawska, Krystian Pluta, Katarzyna Kaleta-Kuratewicz, Jan P Madej, Piotr Kuropka, Jan Kuryszko
The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated. Clobederm®, containing clobetasol propioniate, served as a reference drug. The application of the compounds led to thinning of the epidermis and reduction of the cell layers. The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis...
April 16, 2018: International Immunopharmacology
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