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Sidestream dark field imaging

Anthonius F J de Bruin, Arthur Tavy, Koene van der Sloot, Anke Smits, Bert Van Ramshorst, Christiaan E Boerma, Peter Kars, Peter G Noordzij, Djamila Boerma, Mat van Iterson
AIM: To investigate whether an image acquisition stabilizer (IAS) mounted on the sidestream dark field camera (SDF) during gastrointestinal surgery improves image stability and acquisition. METHODS: Serosal SDF imaging was compared with SDF imaging combined with an IAS (SDF + IAS) during gastrointestinal surgery. Stability was assessed as the image drift in pixels and the time to obtain stable images. The success rate was determined as the percentage of analyzable images after recording...
October 4, 2016: Journal of Vascular Research
Sebastien Tanaka, Etienne Escudier, Sophie Hamada, Anatole Harrois, Pierre Etienne Leblanc, Eric Vicaut, Jacques Duranteau
OBJECTIVES: The effects of RBC transfusion on microvascular perfusion are not well documented. We investigated the effect of RBC transfusion on sublingual microcirculation in hemorrhagic shock patients. DESIGN: Prospective, preliminary observational study. SETTINGS: A 28-bed, surgical ICU in a university hospital. PATIENTS: Fifteen hemorrhagic shock patients requiring RBC transfusion. INTERVENTION: Transfusion of one unit of RBCs...
September 15, 2016: Critical Care Medicine
Xu Liu, Kai Zhang, Wei Wang, Guohao Xie, Baoli Cheng, Yan Wang, Yaoqin Hu, Xiangming Fang
OBJECTIVES: To compare the effects of dexmedetomidine and propofol on sublingual microcirculation in patients after cardiac surgery. DESIGN: A prospective, randomized, single-blind study. SETTING: University hospital. PARTICIPANTS: Adult patients undergoing elective valve surgery with cardiopulmonary bypass. INTERVENTIONS: On arrival in the intensive care unit (ICU), patients were assigned randomly to receive either dexmedetomidine (0...
May 25, 2016: Journal of Cardiothoracic and Vascular Anesthesia
Elisa Damiani, Can Ince, Claudia Scorcella, Roberta Domizi, Andrea Carsetti, Nicoletta Mininno, Silvia Pierantozzi, Erica Adrario, Rocco Romano, Paolo Pelaia, Abele Donati
We aimed to assess the impact of image quality on microcirculatory evaluation with sidestream dark-field (SDF) videomicroscopy in critically ill patients and explore factors associated with low video quality. This was a retrospective analysis of a single-centre prospective observational study. Videos of the sublingual microcirculation were recorded using SDF videomicroscopy in 100 adult patients within 12 h from admittance to the intensive care unit and every 24 h until discharge/death. Parameters of vessel density and perfusion were calculated offline for small vessels...
August 18, 2016: Journal of Clinical Monitoring and Computing
Vanessa Roberta Rodrigues Cunha, Rodrigo Barbosa de Souza, Ana Maria Cristina Rebello Pinto da Fonseca Martins, Ivan Hong Jun Koh, Vera Regina Leopoldo Constantino
Biocompatibility of layered double hydroxides (LDHs), also known as hydrotalcite-like materials or double metal hydroxides, was investigated by in vivo assays via intramuscular tablets implantation in rat abdominal wall. The tablets were composed by chloride ions intercalated into LDH of magnesium/aluminum (Mg2Al-Cl) and zinc/aluminum (Zn2Al-Cl). The antigenicity and tissue integration capacity of LDHs were assessed histologically after 7 and 28 days post-implantation. No fibrous capsule nearby the LDH was noticed for both materials as well any sign of inflammatory reactions...
2016: Scientific Reports
Tomas Tamosuitis, Andrius Pranskunas, Neringa Balciuniene, Vidas Pilvinis, E Christiaan Boerma
BACKGROUND: The conjunctival microcirculation has potential as a window to cerebral perfusion due to related blood supply, close anatomical proximity and easy accessibility for microcirculatory imaging technique, such as sidestream dark field (SDF) imaging. Our study aims to evaluate conjunctival and sublingual microcirculation in brain dead patients and to compare it with healthy volunteers in two diametrically opposed conditions: full stop versus normal arterial blood supply to the brain...
2016: BMC Neurology
Petra Krupičková, Mikuláš Mlček, Michal Huptych, Zuzana Mormanová, Tomáš Bouček, Tomáš Belza, Stanislav Lacko, Miloš Černý, Petr Neužil, Otomar Kittnar, Aleš Linhart, Jan Bělohlávek
BACKGROUND: Current research highlights the role of microcirculatory disorders in post-cardiac arrest patients. Affected microcirculation shows not only dissociation from systemic hemodynamics but also strong connection to outcome of these patients. However, only few studies evaluated microcirculation directly during cardiac arrest (CA) and cardiopulmonary resuscitation (CPR). The aim of our experimental study in a porcine model was to describe sublingual microcirculatory changes during CA and CPR using recent videomicroscopic technology and provide a comparison to parameters of global hemodynamics...
2016: Journal of Translational Medicine
Sophie Yacoub, Phung Khanh Lam, Le Hoang Mai Vu, Thi Lien Le, Ngo Thanh Ha, Tran Thi Toan, Nguyen Thu Van, Nguyen Than Ha Quyen, Huynh Thi Le Duyen, Nguyen Van Kinh, Annette Fox, Juthathip Mongkolspaya, Marcel Wolbers, Cameron Paul Simmons, Gavin Robert Screaton, Heiman Wertheim, Bridget Wills
BACKGROUND: The hallmark of severe dengue is increased microvascular permeability, but alterations in the microcirculation and their evolution over the course of dengue are unknown. METHODS: We conducted a prospective observational study to evaluate the sublingual microcirculation using side-stream dark-field imaging in patients presenting early (<72 hours after fever onset) and patients hospitalized with warning signs or severe dengue in Vietnam. Clinical findings, microvascular function, global hemodynamics assessed with echocardiography, and serological markers of endothelial activation were determined at 4 time points...
September 1, 2016: Journal of Infectious Diseases
Roel Hubert Louis Haeren, Steffi Elisabeth Maria van de Ven, Marcus Anna Maria Jacobus van Zandvoort, Hans Vink, Jacobus Johannes van Overbeeke, Govert Hoogland, Kim Rijkers
The glycocalyx is a gel-like layer lining the luminal surface of the endothelium. The glycocalyx exerts an important barrier role because it prevents exposure of plasma components to the endothelial surface. Disruption of the glycocalyx by local inflammation or ischemia results in decreased glycocalyx thickness which is associated with a number of vascular diseases. The cerebrovascular glycocalyx has sparsely been studied, but is of great interest because of its potential role in cerebrovascular disease. In this review, we describe all existing techniques to visualize the glycocalyx and designate techniques that may be suitable for studying the cerebrovascular glycocalyx...
2016: Current Neurovascular Research
Annemarie Nadort, Koen Kalkman, Ton G van Leeuwen, Dirk J Faber
Laser speckle flowmetry suffers from a debated quantification of the inverse relation between decorrelation time (τc) and blood flow velocity (V), i.e. 1/τc = αV. Using a modified microcirculation imager (integrated sidestream dark field - laser speckle contrast imaging [SDF-LSCI]), we experimentally investigate on the influence of the optical properties of scatterers on α in vitro and in vivo. We found a good agreement to theoretical predictions within certain limits for scatterer size and multiple scattering...
2016: Scientific Reports
Ferit Kulalı, Ebru Ergenekon, Selma Aktas, Ebru Kazancı, Sezin Unal, İbrahim Hirfanoğlu, Esra Önal, Canan Türkyılmaz, Esin Koç, Yıldız Atalay
BACKGROUND: Microcirculation is an important component of hemodynamic physiology . It can be assessed simply by clinical scores or by a variety of techniques including sidestream dark field (SDF) imaging and peripheral perfusion index (PI) measurements. Mode of delivery may have affects on microcirculation during transitional period. The aim of this study was to compare skin microcirculation in newborns born via vaginal delivery (VD) or cesarian section (C/S). METHODS: Term healthy newborns not requiring NICU admission were included...
April 28, 2016: Journal of Maternal-fetal & Neonatal Medicine
Yu-Chang Yeh, Chun-Yu Wu, Ya-Jung Cheng, Chih-Min Liu, Jong-Kai Hsiao, Wing-Sum Chan, Zong-Gin Wu, Linda Chia-Hui Yu, Wei-Zen Sun
BACKGROUND: Dexmedetomidine reduces cytokine production in septic patients and reduces inflammation and mortality in experimental models of endotoxemia and sepsis. This study investigated whether dexmedetomidine attenuates endothelial dysfunction, intestinal microcirculatory dysfunction, and intestinal epithelial barrier disruption in endotoxemic rats. METHODS: Ninety-two male Wistar rats were randomly assigned to the following four groups: (1) Sham; (2) lipopolysaccharide, received IV lipopolysaccharide 15 and 10 mg/kg at 0 and 120 min; (3) dexmedetomidine, received IV dexmedetomidine for 240 min; and (4) lipopolysaccharide + dexmedetomidine, received both lipopolysaccharide and dexmedetomidine...
August 2016: Anesthesiology
F Sui, Y Zheng, W-X Li, J-L Zhou
OBJECTIVE: The purpose of the study is to further investigate the effects of increased intra-abdominal pressure (IAP) on renal hemodynamics and renal perfusion, and to evaluate the renal cortical and sublingual microcirculation by sidestream dark field (SDF) imaging, both with a porcine model. MATERIALS AND METHODS: IAP was increased stepwise to 10, 15, 20, 25 mmHg, during which hemodynamic parameters, urinary output, renal contrast-enhanced ultrasound (CEUS), sublingual and renal SDF videos were all recorded from 12 pigs...
2016: European Review for Medical and Pharmacological Sciences
Carina M Schinagl, Zuzana H Mormanova, Alexandra Puchwein-Schwepcke, Irene Schmid, Orsolya Genzel-Boroviczény
UNLABELLED: Red blood cell transfusion can improve but also might temporarily reduce the microcirculation. The buccal microcirculation was visualized and total vessel density (TVD) determined with sidestream dark field imaging in 19 pediatric anemic (Hb 7.2 g/dL, 95 % CI 6.5-7.9) oncology or hematology patients receiving red blood cell transfusions (Tx) and in 18 age-matched healthy non-anemic controls. After transfusion, Hb (8.0 g/dL, 95 % CI 7.3-8.6) and TVD increased (14.7 ± 1...
June 2016: European Journal of Pediatrics
Junyuan Wu, Chunsheng Li, Wei Yuan
AIM OF THE STUDY: To examine the effects of Shenfu injection (SFI) on macrocirculation and microcirculation during ventricular fibrillation (VF) and cardiopulmonary resuscitation (CPR). MATERIALS AND METHODS: Sixteen female Landrace pigs were used in this study. After anesthesia, coronary perfusion pressure (CPP) was measured, and then the abdominal cavity was opened to observe the mesenteric microcirculation with the aid of sidestream dark field imaging. Following the guidelines, we determined microvascular flow index, perfused vessel density and proportion of perfused vessels both for large (diameter>20 μm) and small (diameter<20 μm) microvessels...
March 2, 2016: Journal of Ethnopharmacology
Edward Gilbert-Kawai, Jonny Coppel, Vassiliki Bountziouka, Can Ince, Daniel Martin
BACKGROUND: The 'Cytocam' is a third generation video-microscope, which enables real time visualisation of the in vivo microcirculation. Based upon the principle of incident dark field (IDF) illumination, this hand held computer-controlled device was designed to address the technical limitations of its predecessors, orthogonal polarization spectroscopy and sidestream dark field (SDF) imaging. In this manuscript, we aimed to compare the quality of sublingual microcirculatory image acquisition between the IDF and SDF devices...
2016: BMC Medical Imaging
Yann-Leei L Lee, Jon D Simmons, Mark N Gillespie, Diego F Alvarez, Richard P Gonzalez, Sidney B Brevard, Mohammad A Frotan, Andrew M Schneider, William O Richards
Achieving adequate perfusion is a key goal of treatment in severe trauma; however, tissue perfusion has classically been measured by indirect means. Direct visualization of capillary flow has been applied in sepsis, but application of this technology to the trauma population has been limited. The purpose of this investigation was to compare the efficacy of standard indirect measures of perfusion to direct imaging of the sublingual microcirculatory flow during trauma resuscitation. Patients with injury severity scores >15 were serially examined using a handheld sidestream dark-field video microscope...
December 2015: American Surgeon
A F J de Bruin, V N N Kornmann, K van der Sloot, J L van Vugt, M P Gosselink, A Smits, B Van Ramshorst, E C Boerma, P G Noordzij, D Boerma, M van Iterson
AIM: The study aimed to describe the serosal microcirculation of the human bowel using sidestream dark field imaging, a microscopic technique using polarized light to visualize erythrocytes through capillaries. We also compared its feasibility to the current practice of sublingual microcirculatory assessment. METHOD: In 17 patients sidestream dark field measurements were performed during gastrointestinal surgery. Microcirculatory parameters like microvascular flow index (MFI), proportion of perfused vessels (PPV), perfused vessel density (PVD) and total vessel density (TVD) were determined for every patient, sublingually and on the bowel serosa...
March 2016: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
Xin An, Heng Zhang, Yini Sun, Xiaochun Ma
OBJECT: This study aimed at exploring what level of the microcirculation alteration could weaken the decrease of ScvO2 (or the increase of O2ER) and further result in an abnormally elevated ScvO2. METHODS: Beagles were randomly assigned into control (n = 5) and shock group (n = 5). The canines in shock group were intravenously injected with live E. coli (3.5×108 cfu/kg), and the ones in control group were injected with sterile saline. The experiment continued to the animals' death or for a maximum of 24 hours...
September 12, 2016: Clinical Hemorheology and Microcirculation
Vanina S Kanoore Edul, Can Ince, Alejandro Risso Vazquez, Paolo N Rubatto, Emilio D Valenzuela Espinoza, Sebastián Welsh, Carolina Enrico, Arnaldo Dubin
RATIONALE: In normodynamic septic shock, the quantitative assessment of sublingual microcirculation has shown decreases in perfused vascular density and red blood cell velocity. However, no studies have been performed in hyperdynamic septic shock. OBJECTIVES: To characterize the microcirculatory patterns and rule out the presence of fast red blood cell velocity in patients with hyperdynamic septic shock. METHODS: We prospectively evaluated the sublingual microcirculation in healthy volunteers (n = 20) and in patients with hyperdynamic (n = 20) and normodynamic (n = 20) septic shock...
February 2016: Annals of the American Thoracic Society
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