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https://www.readbyqxmd.com/read/27844135/cytosolic-proteome-profiling-of-monocytes-for-male-osteoporosis
#1
W Zhu, H Shen, J-G Zhang, L Zhang, Y Zeng, H-L Huang, Y-C Zhao, H He, Y Zhou, K-H Wu, Q Tian, L-J Zhao, F-Y Deng, H-W Deng
: In male Caucasians with discordant hip bone mineral density (BMD), we applied the subcellular separation and proteome profiling to investigate the monocytic cytosol. Three BMD-associated proteins (ALDOA, MYH14, and Rap1B) were identified based on multiple omics evidence, and they may influence the pathogenic mechanisms of osteoporosis by regulating the activities of monocytes. INTRODUCTION: Osteoporosis is a serious public health problem, leading to significant mortality not only in aging females but also in males...
November 14, 2016: Osteoporosis International
https://www.readbyqxmd.com/read/27793029/g6pc3-aldoa-and-cs-induction-accompanies-mir-122-down-regulation-in-the-mechanical-asphyxia-and-can-serve-as-hypoxia-biomarkers
#2
Yan Zeng, Yehui Lv, Li Tao, Jianlong Ma, Heng Zhang, Hongmei Xu, Bi Xiao, Qun Shi, Kaijun Ma, Long Chen
Hypoxia influences different cellular biological processes. To reveal the dynamics of hypoxia's effects on miRNA regulation in vivo, we examined the expression levels of all miRNAs in human brain and heart specimens from cases of mechanical asphyxia compared with those from cases of craniocerebral injury and hemorrhagic shock. We further validated differently expressed miRNAs in another 84 human specimens and rat models. We found that mir-122 was significantly down-regulated and that its putative targets G6PC3, ALDOA and CS were increased in the brain and cardiac tissues in cases of mechanical asphyxia compared with craniocerebral injury and hemorrhagic shock...
October 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27688707/novel-biomarker-candidates-for-colorectal-cancer-metastasis-a-meta-analysis-of-in-vitro-studies
#3
Nguyen Phuoc Long, Wun Jun Lee, Nguyen Truong Huy, Seul Ji Lee, Jeong Hill Park, Sung Won Kwon
Colorectal cancer (CRC) is one of the most common and lethal cancers. Although numerous studies have evaluated potential biomarkers for early diagnosis, current biomarkers have failed to reach an acceptable level of accuracy for distant metastasis. In this paper, we performed a gene set meta-analysis of in vitro microarray studies and combined the results from this study with previously published proteomic data to validate and suggest prognostic candidates for CRC metastasis. Two microarray data sets included found 21 significant genes...
2016: Cancer Informatics
https://www.readbyqxmd.com/read/27684953/quantitative-proteomic-profiling-the-molecular-signatures-of-annexin-a5-in-lung-squamous-carcinoma-cells
#4
Bing Sun, Yuxin Bai, Liyuan Zhang, Linlin Gong, Xiaoyu Qi, Huizhen Li, Faming Wang, Xinming Chi, Yulin Jiang, Shujuan Shao
Lung cancer remains the leading cancer killer around the world. It's crucial to identify newer mechanism-based targets to effectively manage lung cancer. Annexin A5 (ANXA5) is a protein kinase C inhibitory protein and calcium dependent phospholipid-binding protein, which may act as an endogenous regulator of various pathophysiological processes. However, its molecular mechanism in lung cancer remains poorly understood. This study was designed to determine the mechanism of ANXA5 in lung cancer with a hope to obtain useful information to provide a new therapeutic target...
2016: PloS One
https://www.readbyqxmd.com/read/27261507/definition-of-a-novel-feed-forward-mechanism-for-glycolysis-hif1%C3%AE-signaling-in-hypoxic-tumors-highlights-aldolase-a-as-a-therapeutic-target
#5
Geoffrey Grandjean, Petrus R de Jong, Brian P James, Mei Yee Koh, Robert Lemos, John Kingston, Alexander Aleshin, Laurie A Bankston, Claudia P Miller, Eun Jeong Cho, Ramakrishna Edupuganti, Ashwini Devkota, Gabriel Stancu, Robert C Liddington, Kevin N Dalby, Garth Powis
The hypoxia-inducible transcription factor HIF1α drives expression of many glycolytic enzymes. Here, we show that hypoxic glycolysis, in turn, increases HIF1α transcriptional activity and stimulates tumor growth, revealing a novel feed-forward mechanism of glycolysis-HIF1α signaling. Negative regulation of HIF1α by AMPK1 is bypassed in hypoxic cells, due to ATP elevation by increased glycolysis, thereby preventing phosphorylation and inactivation of the HIF1α transcriptional coactivator p300. Notably, of the HIF1α-activated glycolytic enzymes we evaluated by gene silencing, aldolase A (ALDOA) blockade produced the most robust decrease in glycolysis, HIF-1 activity, and cancer cell proliferation...
July 15, 2016: Cancer Research
https://www.readbyqxmd.com/read/27152194/molecular-characterization-of-gleason-patterns-3-and-4-prostate-cancer-using-reverse-warburg-effect-associated-genes
#6
Ilinca Georgescu, Robert J Gooding, R Christopher Doiron, Andrew Day, Shamini Selvarajah, Chris Davidson, David M Berman, Paul C Park
BACKGROUND: Gleason scores (GS) 3+3 and 3+4 prostate cancers (PCa) differ greatly in their clinical courses, with Gleason pattern (GP) 4 representing a major independent risk factor for cancer progression. However, Gleason grade is not reliably ascertained by diagnostic biopsy, largely due to sampling inadequacies, subjectivity in the Gleason grading procedure, and a lack of more objective biomarker assays to stratify prostate cancer aggressiveness. In most aggressive cancer types, the tumor microenvironment exhibits a reciprocal pro-tumorigenic metabolic phenotype consistent with the reverse Warburg effect (RWE)...
2016: Cancer & Metabolism
https://www.readbyqxmd.com/read/27012587/in-silico-prediction-of-micrornas-on-fluoride-induced-sperm-toxicity-in-mice
#7
Azhwar Raghunath, Dhivyalakshmi Jeyabaskar, Kiruthika Sundarraj, Lakshmikanthan Panneerselvam, Ekambaram Perumal
Fluorosis is an endemic global problem causing male reproductive impairment. F mediates male reproductive toxicity in mice down-regulating 63 genes involved in diverse biological processes - apoptosis, cell cycle, cell signaling, chemotaxis, electron transport, glycolysis, oxidative stress, sperm capacitation and spermatogenesis. We predicted the miRNAs down-regulating these 63 genes using TargetScan, DIANA and MicroCosm. The prediction tools identified 3059 miRNAs targeting 63 genes. Of the predicted interactions, 11 miRNAs (mmu-miR-103, -107, -122, -188a, -199a-5p, -205, -340-5p, -345-3p, -452-5p, -499, -878-3p) were commonly found in the three tools utilized and seven miRNAs (miR-9-5p, miR-511-3p, miR-7b-5p, miR-30e-5p, miR-17-5p, miR-122-5p and miR-541-5p) targeting six genes (Traf3, Rock2, Rgs8, Atp1b2, Cacna2d1 and Aldoa) were already validated experimentally in mice...
March 21, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/26854714/aldoa-functions-as-an-oncogene-in-the-highly-metastatic-pancreatic-cancer
#8
Shunrong Ji, Bo Zhang, Jiang Liu, Yi Qin, Chen Liang, Si Shi, Kaizhou Jin, Dingkong Liang, Wenyan Xu, Huaxiang Xu, Wenquan Wang, Chuntao Wu, Liang Liu, Chen Liu, Jin Xu, Quanxing Ni, Xianjun Yu
Pancreatic cancer is an aggressive and devastating disease that is characterized by uncontrolled progression, invasiveness and resistance to conventional treatment. In the past decades, much effort has been given to cancer genetics and pathological classification of this disease. Our previous study has uncovered a subgroup of patients with poor outcome, which is characterized by serum signature of CEA(+)/CA125(+)/CA19-9 ≥ 1000 U/mL; however, the underlying biology mechanism remains poorly understood...
April 28, 2016: Cancer Letters
https://www.readbyqxmd.com/read/26781521/verification-of-suitable-and-reliable-reference-genes-for-quantitative-real-time-pcr-during-adipogenic-differentiation-in-porcine-intramuscular-stromal-vascular-cells
#9
X Li, K Huang, F Chen, W Li, S Sun, X-E Shi, G Yang
Intramuscular fat (IMF) is an important trait influencing meat quality, and intramuscular stromal-vascular cell (MSVC) differentiation is a key factor affecting IMF deposition. Quantitative real-time PCR (qPCR) is often used to screen the differentially expressed genes during differentiation of MSVCs, where proper reference genes are essential. In this study, we assessed 31 of previously reported reference genes for their expression suitability in porcine MSVCs derived form longissimus dorsi with qPCR. The expression stability of these genes was evaluated using NormFinder, geNorm and BestKeeper algorithms...
June 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/26333492/-angiopoietin-like-4-modulates-aldolase-a-expression-in-human-melanoma-cell-in-a-pkc-dependent-manner
#10
Yang Sun, Xiancheng Wang, Borong Fang, Xiang Xiong, Jianhong Long
OBJECTIVE: To investigate the association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell. 
 METHODS: Overexpression or knockdown of ANGPTL4 was performed in WM-115 or WM-266-4 cells, respectively. The expression of ANGPTL4 and ALDOA was measured by RT-PCR and Western blot, respectively. The promoter activity of ALDOA gene was determined by luciferase assay.
 RESULTS: The promoter activity of ALDOA gene and the expression of ALDOA (mRNA and protein) were increased or decreased in the melanoma cells with overexpression or knockdown of ANGPTL4, which was blocked by selective protein kinase C (PKC) inhibitor or restored by PKC agonist, respectively...
August 2015: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/26169282/the-usability-of-a-15-gene-hypoxia-classifier-as-a-universal-hypoxia-profile-in-various-cancer-cell-types
#11
Brita Singers Sørensen, Anders Knudsen, Catja Foged Wittrup, Steffen Nielsen, Ninna Aggerholm-Pedersen, Morten Busk, Michael Horsman, Morten Høyer, Pierre Nourdine Bouchelouche, Jens Overgaard, Jan Alsner
BACKGROUND AND PURPOSE: A 15-gene hypoxia profile has previously demonstrated to have both prognostic and predictive impact for hypoxic modification in squamous cell carcinoma of the head and neck. This gene expression profile may also have a prognostic value in other histological cancer types, and could potentially have a function as a universal hypoxia profile. The hypoxia induced upregulation of the included genes, and the validity of the previously used reference genes was established in this study, in a range of different cell lines representing carcinomas of the prostate, colon, and esophagus...
September 2015: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
https://www.readbyqxmd.com/read/26083354/genome-wide-specific-selection-in-three-domestic-sheep-breeds
#12
Huihua Wang, Li Zhang, Jiaxve Cao, Mingming Wu, Xiaomeng Ma, Zhen Liu, Ruizao Liu, Fuping Zhao, Caihong Wei, Lixin Du
BACKGROUND: Commercial sheep raised for mutton grow faster than traditional Chinese sheep breeds. Here, we aimed to evaluate genetic selection among three different types of sheep breed: two well-known commercial mutton breeds and one indigenous Chinese breed. RESULTS: We first combined locus-specific branch lengths and di statistical methods to detect candidate regions targeted by selection in the three different populations. The results showed that the genetic distances reached at least medium divergence for each pairwise combination...
2015: PloS One
https://www.readbyqxmd.com/read/26063603/alteration-of-sperm-protein-profile-induced-by-cigarette-smoking
#13
Xiaohui Chen, Wangjie Xu, Maohua Miao, Zijue Zhu, Jingbo Dai, Zhong Chen, Peng Fang, Junqing Wu, Dongsheng Nie, Lianyun Wang, Zhaoxia Wang, Zhongdong Qiao, Huijuan Shi
Cigarette smoking is associated with lower semen quality, but how cigarette smoking changes the semen quality remains unclear. The aim of this study was to screen the differentially expressed proteins in the sperm of mice with daily exposure to cigarette smoke. The 2D gel electrophoresis (2DE) and mass spectrometry (MS) analyses results showed that the mouse sperm protein profile was altered by cigarette smoking. And 22 of the most abundant proteins that correspond to differentially expressed spots in 2DE gels of the sperm samples were identified...
July 2015: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/25977172/effect-of-stat3-inhibition-on-the-metabolic-switch-in-a-highly-stat3-activated-lymphoma-cell-line
#14
Yasuto Akiyama, Akira Iizuka, Akiko Kume, Masaru Komiyama, Kenichi Urakami, Tadashi Ashizawa, Haruo Miyata, Maho Omiya, Masatoshi Kusuhara, Ken Yamaguchi
BACKGROUND: Signal transducer and activator of transcription (STAT)3 is involved in a metabolic shift in cancer cells, the Warburg effect through its pro-oncogenic activity. To develop efficient STAT3 inhibitors against cancer cells, novel proteomic and metabolic target molecules need to be explored using multi-omics approaches in the context of STAT3 gene inhibition-mediated tumor growth suppression. MATERIALS AND METHODS: We found that short hairpin (sh)RNA-mediated STAT3 inhibition suppressed tumor growth in a highly STAT3-activated lymphoma cell line, SCC-3 cells, and we investigated the effect of STAT3 inhibition on metabolic switching using 2-dimensional differential gel electrophoresis and capillary electrophoresis-time of flight-mass spectrometry...
May 2015: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/25932951/comprehensive-analysis-of-glycolytic-enzymes-as-therapeutic-targets-in-the-treatment-of-glioblastoma
#15
Morgane Sanzey, Siti Aminah Abdul Rahim, Anais Oudin, Anne Dirkse, Tony Kaoma, Laurent Vallar, Christel Herold-Mende, Rolf Bjerkvig, Anna Golebiewska, Simone P Niclou
Major efforts have been put in anti-angiogenic treatment for glioblastoma (GBM), an aggressive and highly vascularized brain tumor with dismal prognosis. However clinical outcome with anti-angiogenic agents has been disappointing and tumors quickly develop escape mechanisms. In preclinical GBM models we have recently shown that bevacizumab, a blocking antibody against vascular endothelial growth factor, induces hypoxia in treated tumors, which is accompanied by increased glycolytic activity and tumor invasiveness...
2015: PloS One
https://www.readbyqxmd.com/read/25687883/metabolic-tumor-burden-is-associated-with-major-oncogenomic-alterations-and-serum-tumor-markers-in-patients-with-resected-pancreatic-cancer
#16
Si Shi, Shunrong Ji, Yi Qin, Jin Xu, Bo Zhang, Wenyan Xu, Jiang Liu, Jiang Long, Chen Liu, Liang Liu, Quanxing Ni, Xianjun Yu
Pancreatic cancer is an aggressive and lethal disease with an overall 5-year survival rate of only 5%. Studies have demonstrated the ability of (18)F-fludrodeoxyglucose ((18)F-FDG) positron emission tomography/computed tomography (PET/CT) to measure the metabolic tumor burden in patients with various tumors, including pancreatic cancer. In a previous study, we investigated the predictive significance of the metabolic tumor burden in terms of the metabolic tumor volume (MTV) and total lesion glycolysis (TLG)...
May 1, 2015: Cancer Letters
https://www.readbyqxmd.com/read/25550045/selection-of-appropriate-reference-genes-for-rt-qpcr-analysis-in-berkshire-duroc-landrace-and-yorkshire-pigs
#17
Sang-Je Park, Seul Gi Kwon, Jung Hye Hwang, Da Hye Park, Tae Wan Kim, Chul Wook Kim
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the most reliable molecular biology technique for assessment of mRNA expression levels. However, to obtain the accurate RT-qPCR results, the expression levels of genes of interest should be normalized with appropriate reference genes and optimal numbers of reference genes. In this study, we assessed the expression stability of 15 well-known candidate reference genes (ACTB, ALDOA, B2M, GAPDH, HPAR1, HSPCB, PGK1, POLR2G, PPIA, RPL4, RPS18, SDHA, TBP, TOP2B, and YWHAZ) in seven body tissues (liver, lung, kidney, spleen, stomach, small intestine, and large intestine) of Berkshire, Landrace, Duroc, and Yorkshire pigs using three excel-based programs, geNorm, NormFinder, and BestKeeper...
March 1, 2015: Gene
https://www.readbyqxmd.com/read/25399599/moonlighting-proteins-in-sperm-egg-interactions
#18
REVIEW
François M Petit, Catherine Serres, Jana Auer
Sperm-egg interaction is a highly species-specific step during the fertilization process. The first steps consist of recognition between proteins on the sperm head and zona pellucida (ZP) glycoproteins, the acellular coat that protects the oocyte. We aimed to determine which sperm head proteins interact with ZP2, ZP3 and ZP4 in humans. Two approaches were combined to identify these proteins: immunoblotting human spermatozoa targeted by antisperm antibodies (ASAs) from infertile men and far-Western blotting of human sperm proteins overlaid by each of the human recombinant ZP (hrZP) proteins...
December 2014: Biochemical Society Transactions
https://www.readbyqxmd.com/read/25392908/a-thermolabile-aldolase-a-mutant-causes-fever-induced-recurrent-rhabdomyolysis-without-hemolytic-anemia
#19
Asmaa Mamoune, Michel Bahuau, Yamina Hamel, Valérie Serre, Michele Pelosi, Florence Habarou, Marie-Ange Nguyen Morel, Bertrand Boisson, Sabrina Vergnaud, Mai Thao Viou, Luc Nonnenmacher, Monique Piraud, Patrick Nusbaum, Joseph Vamecq, Norma Romero, Chris Ottolenghi, Jean-Laurent Casanova, Pascale de Lonlay
Aldolase A deficiency has been reported as a rare cause of hemolytic anemia occasionally associated with myopathy. We identified a deleterious homozygous mutation in the ALDOA gene in 3 siblings with episodic rhabdomyolysis without hemolytic anemia. Myoglobinuria was always triggered by febrile illnesses. We show that the underlying mechanism involves an exacerbation of aldolase A deficiency at high temperatures that affected myoblasts but not erythrocytes. The aldolase A deficiency was rescued by arginine supplementation in vitro but not by glycerol, betaine or benzylhydantoin, three other known chaperones, suggesting that arginine-mediated rescue operated by a mechanism other than protein chaperoning...
November 2014: PLoS Genetics
https://www.readbyqxmd.com/read/25379943/comparative-proteomics-analysis-of-gastric-cancer-stem-cells
#20
COMPARATIVE STUDY
Tamami Morisaki, Masakazu Yashiro, Anna Kakehashi, Azusa Inagaki, Haruhito Kinoshita, Tatsunari Fukuoka, Hiroaki Kasashima, Go Masuda, Katsunobu Sakurai, Naoshi Kubo, Kazuya Muguruma, Masaichi Ohira, Hideki Wanibuchi, Kosei Hirakawa
Cancer stem cells (CSCs) are responsible for cancer progression, metastasis, and recurrence. To date, the specific markers of CSCs remain undiscovered. The aim of this study was to identify novel biomarkers of gastric CSCs for clinical diagnosis using proteomics technology. CSC-like SP cells, OCUM-12/SP cells, OCUM-2MD3/SP cells, and their parent OCUM-12 cells and OCUM-2MD3 cells were used in this study. Protein lysates from each cell line were analyzed using QSTAR Elite Liquid Chromatography with Tandem Mass Spectrometry, coupled with isobaric tags for relative and absolute quantitation technology...
2014: PloS One
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