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https://www.readbyqxmd.com/read/28737890/a-difluoroboron-%C3%AE-diketonate-probe-shows-turn-on-near-infrared-fluorescence-specific-for-tau-fibrils
#1
Kwang-Su Park, Mi Kyoung Kim, Yujin Seo, Taewoong Ha, Kyeongha Yoo, Seung Jae Hyeon, Yu Jin Hwang, Junghee Lee, Hoon Ryu, Hyunah Choo, Youhoon Chong
Tau aggregation in neuronal cells has recently received significant attention as a robust predictor of the progression of Alzheimer's disease (AD) because of its proven correlation with the degree of cognitive impairment in AD patients. Accordingly, non-invasive imaging of tau aggregates has been highlighted as a promising diagnostic tool for AD. We have previously identified a tau-specific "turn-on" near-infrared fluorescent (NIRF) probe (1) and, in this study, structural modification was performed to optimize its physicochemical as well as fluorescence properties...
July 24, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28737550/intestinal-cestodes
#2
Camille Webb, Miguel M Cabada
PURPOSE OF REVIEW: Cestodes infections in humans are among the most prevalent parasitosis worldwide. Although tapeworm infection is often asymptomatic, they can be associated with a range of symptoms. The landscape of cestode infections is changing with rapid diagnosis techniques and advanced molecular diagnosis aiding in identification of species specific epidemiology. RECENT FINDINGS: Traditional descriptions of species by location have been challenged with molecular diagnostic techniques, which show variation in distribution of species, thought to be because of globalization and importation of disease...
July 22, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28736873/standardised-molecular-diagnostic-tool-for-the-identification-of-cryptic-species-within-the-bemisia-tabaci-complex
#3
S Elfekih, W T Tay, K Gordon, L Court, P De Barro
BACKGROUND: The whitefly Bemisia tabaci complex harbours over 40 cryptic species that have been placed in 11 phylogenetically distinct clades based on the molecular characterisation of partial mitochondrial DNA COI (mtCOI) gene region. Four cryptic species are currently within the invasive clade i.e. 'MED', 'MEAM1', 'MEAM2' and IO'. Correct identification of these species is a critical step towards implementing reliable measures for plant biosecurity and border protection, however no standardised B...
July 23, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28736855/-lnc-ing-wnt-in-female-reproductive-cancers-therapeutic-potential-of-long-non-coding-rnas-in-wnt-signaling
#4
REVIEW
Mei S Ong, Wanpei Cai, Yi Yuan, Hin C Leong, Tuan Z Tan, Asad Mohammad, Ming L You, Frank Arfuso, Boon C Goh, Sudha Warrier, Gautam Sethi, Nicholas S Tolwinski, Peter E Lobie, Celestial T Yap, Shing C Hooi, Ruby Y Huang, Alan P Kumar
Recent discoveries in the non-coding genome have challenged the original central dogma of molecular biology; non-coding RNAs and related processes have been found to be important in regulating gene expression. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are amongst some that have gained attention recently in human diseases, including cancer, with the involvement of many more ncRNAs waiting to be discovered. Non-coding RNAs (ncRNAs) are a group of ribonucleic acids transcribed from regions of the human genome, which do not become translated into proteins, despite having essential roles in cellular physiology...
July 24, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28736577/optimization-of-proximity-ligation-assay-pla-for-detection-of-protein-interactions-and-fusion-proteins-in-non-adherent-cells-application-to-pre-b-lymphocytes
#5
Lydie Debaize, Hélène Jakobczyk, Anne-Gaëlle Rio, Virginie Gandemer, Marie-Bérengère Troadec
BACKGROUND: Genetic abnormalities, including chromosomal translocations, are described for many hematological malignancies. From the clinical perspective, detection of chromosomal abnormalities is relevant not only for diagnostic and treatment purposes but also for prognostic risk assessment. From the translational research perspective, the identification of fusion proteins and protein interactions has allowed crucial breakthroughs in understanding the pathogenesis of malignancies and consequently major achievements in targeted therapy...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/28736571/evaluation-of-quality-assessment-protocols-for-high-throughput-genome-resequencing-data
#6
REVIEW
Matteo Chiara, Giulio Pavesi
Large-scale initiatives aiming to recover the complete sequence of thousands of human genomes are currently being undertaken worldwide, concurring to the generation of a comprehensive catalog of human genetic variation. The ultimate and most ambitious goal of human population scale genomics is the characterization of the so-called human "variome," through the identification of causal mutations or haplotypes. Several research institutions worldwide currently use genotyping assays based on Next-Generation Sequencing (NGS) for diagnostics and clinical screenings, and the widespread application of such technologies promises major revolutions in medical science...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28736318/psma-targeted-18-f-dcfpyl-pet-ct-in-the-preoperative-staging-of-men-with-high-risk-prostate-cancer-results-of-a-prospective-phase-ii-single-center-study
#7
Michael A Gorin, Steven P Rowe, Hiten D Patel, Igor Vidal, Margarita Mana-Ay, Mehrbod S Javadi, Lilja B Solnes, Ashley E Ross, Edward M Schaeffer, Trinity J Bivalacqua, Alan W Partin, Kenneth J Pienta, Zsolt Szabo, Angelo M De Marzo, Martin G Pomper, Mohamad E Allaf
PURPOSE: To prospectively evaluate the diagnostic performance of PSMA-targeted (18)F-DCFPyL PET/CT in the preoperative staging of men at high risk of harboring metastatic prostate cancer despite a negative conventional staging evaluation. MATERIALS AND METHODS: Men with clinically localized high or very-high risk prostate cancer were imaged with (18)F-DCFPyL PET/CT prior to undergoing radical prostatectomy with a standardized pelvic lymph node dissection. PET/CT scans were interpreted by two blinded nuclear medicine readers and were assessed for inter-reader variability as well as diagnostic accuracy for pelvic lymph node staging...
July 20, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28736315/the-spectrum-of-triple-negative-breast-disease-high-and-low-grade-lesions
#8
REVIEW
Felipe C Geyer, Fresia Pareja, Britta Weigelt, Emad Rakha, Ian O Ellis, Stuart J Schnitt, Jorge S Reis-Filho
Triple-negative breast cancer is viewed clinically as an aggressive subgroup of breast cancer. In fact, the majority of triple-negative breast cancers are poor-prognosis tumors with a complex genomic landscape. However, triple-negative disease is vastly heterogeneous, encompassing multiple entities with marked genetic, transcriptional, histological, and clinical differences, with neoplasms in this group ranging from low- to high-grade. Among the less common low-grade triple-negative lesions, two large subgroups, both with a rather indolent behavior, can be distinguished: a low-grade triple-negative breast neoplasia family, which includes nonobligate precursors of triple-negative breast cancer, and, despite being low-grade, harbor the complex genomic landscape of usual triple-negative breast cancer, and the salivary gland-like tumors of the breast, lacking all of the cardinal molecular features of conventional triple-negative breast cancer and underpinned by specific fusion-genes or hotspot mutations, which may be of diagnostic and possibly therapeutic utility...
July 20, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28736184/attitudes-towards-zika-virus-infection-among-medical-doctors-in-aceh-province-indonesia
#9
Harapan Harapan, Alma Alleta, Samsul Anwar, Abdul M Setiawan, Reza Maulana, Nur Wahyuniati, Muhammad R Ramadana, Ikram Ikram, Sotianingsih Haryanto, Kurnia F Jamil, Ulrich Kuch, Alfonso J Rodríguez-Morales
Zika virus (ZIKV) infection, a public health emergency of international concern, has recently been confirmed in Indonesia. However, to date, there has been no study to assess how prepared healthcare workers in Indonesia are to confront this emerging infectious disease. The aim of this study was to assess the attitudes of medical doctors in Indonesia towards ZIKV infection and its associated explanatory variables. A cross-sectional self-administered online survey was conducted from 3 May to 3 June 2016 in Aceh province, Indonesia...
July 20, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28735527/fibroblast-activating-protein-%C3%AE-expression-in-squamous-cell-carcinoma-of-the-esophagus-in-primary-and-irradiated-tumors-the-use-of-archival-ffpe-material-for-molecular-techniques
#10
Renata Tabola, Magdalena Zaremba-Czogalla, Dagmara Baczynska, Roberto Cirocchi, Kamila Stach, Krzysztof Grabowski, Katarzyna Augoff
There are numerous reports suggesting that fibroblast activating protein-α (FAP-α) plays an important role in invasion of various tumor types. We studied the expression pattern of FAP-α in esophageal squamous cell carcinoma (ESCC) patients who had not been treated primarily and those who had received neoadjuvant radiochemotherapy. Our goal was to establish whether readily available tissue specimens fixed in formalin and stored in paraffin blocks for years might still be a source of FAP-α RNA for PCR analysis...
June 20, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735479/immunohistochemistry-in-acute-myeloid-leukemia
#11
Michael W Cruise
The World Health Organization's Classification of Tumours of Haematopoietic and Lymphoid Tissues (Swerdlow et al. (eds) WHO Classification of tumours of haematopoietic and lymphoid tissues, 4th edn. WHO Press, Lyon, 2008) created a classification scheme incorporating genetic, molecular, morphologic, and immunophenotypic characteristics. The diagnosis of acute myeloid leukemia requires equal to or greater than 20% blasts (except in some cases with specific cytogenetic abnormalities or in erythroleukemia). The diagnostic work up typically includes morphologic, cytochemical, and immunophenotypic features...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735477/molecular-malfeasance-mediating-myeloid-malignancies-the-genetics-of-acute-myeloid-leukemia
#12
Rebecca L King, Adam Bagg
A remarkable number of different, but recurrent, structural cytogenetic abnormalities have been observed in AML, and the 2016 WHO AML classification system incorporates numerous distinct entities associated with translocations or inversions, as well as others associated with single gene mutations into a category entitled "AML with recurrent genetic abnormalities." The AML classification is heavily reliant on cytogenetic and molecular information based on conventional genetic techniques (including karyotype, fluorescence in situ hybridization, reverse transcriptase polymerase chain reaction, single gene sequencing), but large-scale next generation sequencing is now identifying novel mutations...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735241/a-new-integrated-approach-to-analyze-bulk-tank-milk-and-raw-milk-filters-for-the-presence-of-the-e-coli-serogroups-frequently-associated-with-vtec-status
#13
Francesca Albonico, Claudia Gusmara, Theo Gugliotta, Monica Loiacono, Michele Mortarino, Alfonso Zecconi
We optimized a combination of microbiological and molecular methods to quickly identify the presence of the O157 and the six non-O157 serogroups (O26, O45, O103, O111, O121 and O145) most frequently associated with VTEC status, at herd level. The lower detection limit of this methodology is 10(1)CFU/ml for each of the serogroups tested. We tested 67 bulk tank milk (BTM) and raw milk filters (RMF) derived from dairy herds located in Lombardy and Trentino Alto Adige. We identified 3 positive samples and 20 positive samples out of 67 respectively in the BTM and RMF...
July 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28735048/emergence-of-different-acinetobacter-baumannii-clones-in-a-croatian-hospital-and-correlation-with-antibiotic-susceptibility
#14
Ranko Ladavac, Branka Bedenić, Mirna Vranić-Ladavac, Nada Barišić, Natalie Karčić, Karoline Pompe, Antun Ferenčić, Aleksandar Stojanović, Harald Seifert, Stjepan Katić, Paul G Higgins
OBJECTIVES: During routine diagnostic laboratory work, the clinical microbiologist observed an increase of Acinetobacter baumannii isolates with three different carbapenem susceptibility patterns: susceptible, intermediate and resistant. Isolates belonging to the same carbapenem susceptibility phenotype exhibited identical susceptibility/resistance patterns to non-β-lactam antibiotics. This prompted us to analyze the mechanisms of carbapenem-resistance and the molecular epidemiology of the isolates...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28734901/gastroresistant-oral-peptide-for-fluorescence-imaging-of-colonic-inflammation
#15
Paola Luciani, Ander Estella-Hermoso de Mendoza, Tommaso Casalini, Silvia Lang, Kirstin Atrott, Marianne R Spalinger, Anna Pratsinis, Jens Sobek, Isabelle Frey-Wagner, Jens Schumacher, Jean-Christophe Leroux, Gerhard Rogler
The use of molecular markers for inflammation in the gastrointestinal tract could empower optical imaging modalities for early diagnosis and eventually personalized timely treatments. A major hurdle to the widespread use of functional fluorescence imaging is the absence of suitable contrast agents, in particular to be administered via the oral route due to the usual proteolytic susceptibility of the biomarkers. By designing a retro-inverso peptide, starting from a previously described sequence specific for N-cadherin, we achieved resistance to gastrointestinal degradation and even slightly improved specificity towards the target, both in ex vivo and in vivo experimental colitis...
July 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28734366/a-label-free-genetic-biosensor-for-diabetes-based-on-aunps-decorated-ito-with-electrochemiluminescent-signaling
#16
Suyan Zhai, Chen Fang, Jilin Yan, Qun Zhao, Yifeng Tu
The variation of I27L gene closely relates to increasing risk of type 2 diabetes, thus it is greatly significant to develop various methods for its identification or monitoring. We report here a novel label-free electrochemiluminescent (ECL) biosensor for simple and effective determination of I27L gene based on Au nanoparticles functionalized ITO electrode. The ECL technique is employed to monitor the hybridization of DNA strands by measuring the changes of its intensity. Here, the ECL signal was quenched by blocked access of probe (luminol anion) owing to the electrostatic repulsion of negatively charged sensor surface and the space resistance...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734106/insecticide-resistance-in-trialeurodes-vaporariorum-populations-and-novel-diagnostics-for-kdr-mutations
#17
Despoina Evr Kapantaidaki, Eldem Sadikoglou, Dimitra Tsakireli, Vasileios Kampanis, Marianna Stavrakaki, Corinna Schorn, Aris Ilias, Maria Riga, George Tsiamis, Ralf Nauen, George Skavdis, John Vontas, Anastasia Tsagkarakou
BACKGROUND: Neonicotinoids, pyrethroids and ketoenols, are currently used for the control of Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). In this study, insecticide resistance status and mechanisms were investigated by classical bioassays and molecular techniques. RESULTS: Dose response bioassays were performed on 19 Greek populations, among the 35 different whitefly populations used for the whole analysis. Resistance factors scaled up to 207-, 4657- and 59- fold, for imidacloprid, bifenthrin and spiromesifen, respectively...
July 22, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28734086/the-role-of-serum-hyaluronic-acid-determination-in-the-diagnosis-of-liver-fibrosis
#18
Monika Gudowska, Bogdan Cylwik, Lech Chrostek
The common pathway leading to liver fibrosis and cirrhosis is growing deposition of extracellular matrix (ECM). It results from molecular and histological rearrangement of collagens, glycoproteins and hyaluronans. Hyaluronic acid is a chief component of the extracellular matrix of connective tissues and plays the main structural role in the formation of ECM. The most important organ involved in the synthesis of hyaluronic acid is the liver. In this paper the meaning of hyaluronic acid in the diagnostics of liver diseases is discussed...
July 19, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28733961/a-review-of-biomarkers-for-neurodegenerative-disease-will-they-swing-us-across-the-valley
#19
REVIEW
Thomas G Beach
Measures of the severity of cognitive impairment or parkinsonism are the usual endpoints in clinical trials for Alzheimer's disease (AD) and Parkinson's disease (PD), but are critically hampered by their lack of disease sensitivity and specificity. Due to the high failure rate of clinical trials, the rate of regulatory approval for efficacious new drugs has stagnated in the past few decades, with the gap between basic science discovery and clinical application metaphorically termed the "Valley of Death". While the causes for this are probably multiple and complex, the usage of biomarkers as surrogate endpoints, particularly when they are molecularly-specific for the disease, has achieved some success in cancer trials, and it is likely that neurodegenerative disease trials would benefit from the same approach...
July 2017: Neurology and Therapy
https://www.readbyqxmd.com/read/28733840/identification-of-a%C3%A2-rare-coch-mutation-by-whole-exome-sequencing-implications-for-personalized-therapeutic-rehabilitation-in-an-austrian-family-with-non-syndromic-autosomal-dominant-late-onset-hearing-loss
#20
Thomas Parzefall, Alexandra Frohne, Martin Koenighofer, Andreas Kirchnawy, Berthold Streubel, Christian Schoefer, Wolfgang Gstoettner, Klemens Frei, Trevor Lucas
BACKGROUND: Non-syndromic autosomal dominant hearing impairment is characteristically postlingual in onset. Genetic diagnostics are essential for genetic counselling, disease prognosis and understanding of the molecular mechanisms of disease. To date, 36 causative genes have been identified, many in only individual families. Gene selection for genetic screening by traditional methods and genetic diagnosis in autosomal dominant patients has therefore been fraught with difficulty. Whole-exome sequencing provides a powerful tool to analyze all protein-coding genomic regions in parallel, thus allowing the comprehensive screening of all known genes and associated alterations...
July 21, 2017: Wiener Klinische Wochenschrift
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