keyword
MENU ▼
Read by QxMD icon Read
search

Human factors

keyword
https://www.readbyqxmd.com/read/29224158/methylation-specific-pcr
#1
João Ramalho-Carvalho, Rui Henrique, Carmen Jerónimo
Cytosine methylation is a DNA modification generally associated with transcriptional silencing. Factors that regulate methylation have been linked to human disease, yet how they contribute to malignancies remains largely unknown. Methylation of DNA can change the functional state of regulatory regions, but does not change the Watson-Crick base pairing of cytosine. Moreover, sequence symmetry of CpGs enables propagation of the methyl mark through cell division. This potential for inheritance coupled with the fact that DNA methylation patterns change during development and disease partially explains the interest in DNA methylation as a memory module...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224137/considerations-for-design-and-analysis-of-dna-methylation-studies
#2
Karin B Michels, Alexandra M Binder
The number of epigenetic studies is exponentially increasing. There is anticipation that DNA methylation may close gaps in our understanding of disease etiology, and how certain risk factors affect health and disease, but also that it has potential as a biomarker for disease. Human DNA methylation studies require careful considerations for design and analysis including population and tissue selection, population stratification, cell heterogeneity, confounding, temporality, sample size, appropriate statistical analysis, and validation of results...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224090/men-are-from-mars-women-are-from-venus-sex-differences-in-insulin-action-and-secretion
#3
Ananda Basu, Simmi Dube, Rita Basu
Sex difference plays a substantial role in the regulation of glucose metabolism in healthy glucose-tolerant humans. The factors which may contribute to the sex-related differences in glucose metabolism include differences in lifestyle (diet and exercise), sex hormones, and body composition. Several epidemiological and observational studies have noted that impaired glucose tolerance is more common in women than men. Some of these studies have attributed this to differences in body composition, while others have attributed impaired insulin sensitivity as a cause of impaired glucose tolerance in women...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29224008/mirnas-mediate-gdnf-induced-proliferation-and-migration-of-glioma-cells
#4
Bao-Le Zhang, Fu-Lu Dong, Ting-Wen Guo, Xiao-He Gu, Lin-Yan Huang, Dian-Shuai Gao
BACKGROUND/AIMS: Glial cell line-derived neurotrophic factor (GDNF) is an important factor promoting invasive glioma growth. This study was performed to reveal a unique mechanism of glioma cell proliferation and migration. METHODS: Human U251 glioma cells were used to screen the optimal GDNF concentration and treatment time to stimulate proliferation and migration. MicroRNA (MiRNA) expression profiles were detected by microarray and confirmed by real-time polymerase chain reaction (PCR)...
December 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29223976/zinc-detoxification-a-functional-genomics-and-transcriptomics-analysis-in-drosophila-melanogaster-cultured-cells
#5
Stephanie E Mohr, Kirstin Rudd, Yanhui Hu, Wei R Song, Quentin Gilly, Michael Buckner, Benjamin E Housden, Colleen Kelley, Jonathan Zirin, Rong Tao, Gabriel Amador, Katarzyna Sierzputowska, Aram Comjean, Norbert Perrimon
Cells require some metals, such as zinc and manganese, but excess levels of these metals can be toxic. As a result, cells have evolved complex mechanisms for maintaining metal homeostasis and surviving metal intoxication. Here, we present the results of a large-scale functional genomic screen in Drosophila cultured cells for modifiers of zinc chloride toxicity, together with transcriptomics data for wildtype or genetically zinc-sensitized cells challenged with mild zinc chloride supplementation. Altogether, we identified 47 genes for which knockdown conferred sensitivity or resistance to toxic zinc or manganese chloride treatment, and more than 1800 putative zinc-responsive genes...
December 9, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29223930/frequency-selectivity-in-macaque-monkeys-measured-using-a-notched-noise-method
#6
Jane A Burton, Margit E Dylla, Ramnarayan Ramachandran
The auditory system is thought to process complex sounds through overlapping bandpass filters. Frequency selectivity as estimated by auditory filters has been well quantified in humans and other mammalian species using behavioral and physiological methodologies, but little work has been done to examine frequency selectivity in nonhuman primates. In particular, knowledge of macaque frequency selectivity would help address the recent controversy over the sharpness of cochlear tuning in humans relative to other animal species...
November 28, 2017: Hearing Research
https://www.readbyqxmd.com/read/29223854/baicalein-improves-liver-inflammation-in-diabetic-db-db-mice-by-regulating-hmgb1-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#7
Huafeng Yin, Lihao Huang, Ting Ouyang, Lvyi Chen
The current study was designed to investigate the hepatoprotective effects and possible mechanisms of Baicalein (BA) on the diabetic liver injury in vivo and in vitro. The results exhibited that BA significantly restored the blood glucose in oral glucose tolerance test (OGTT) and inhibited the levels of insulin, alanine aminotransferase (ALT), aspartate aminotransaminase (AST), total cholesterol (TC) and triglyceride (TG) in C57BL/KsJ-db/db mice. Moreover, BA strikingly attenuated the extent of steatosis in the liver tissues of diabetic mice...
December 7, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29223822/exposure-to-acrylamide-induces-cardiac-developmental-toxicity-in-zebrafish-during-cardiogenesis
#8
Mengmeng Huang, Jingjing Jiao, Jun Wang, Zhidan Xia, Yu Zhang
Acrylamide (AA), an environmental pollutant, has been linked to neurotoxicity, genotoxicity and carcinogenicity. AA is widely used to synthesize polymers for industrial applications, is widely found in Western-style carbohydrate-rich foods and cigarette smoke, and can also be detected in human umbilical cord blood and breast milk. This is the first study that demonstrated the cardiac developmental toxicity of AA in zebrafish embryos. Post-fertilization exposure to AA caused a clearly deficient cardiovascular system with a shrunken heart and abortive morphogenesis and function...
December 7, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29223770/rhythmic-facilitation-of-sensory-processing-a-critical-review
#9
REVIEW
Saskia Haegens, Elana Zion Golumbic
Here we review the role of brain oscillations in sensory processing. We examine the idea that neural entrainment of intrinsic oscillations underlies the processing of rhythmic stimuli in the context of simple isochronous rhythms as well as in music and speech. This has been a topic of growing interest over recent years; however, many issues remain highly controversial: how do fluctuations of intrinsic neural oscillations-both spontaneous and entrained to external stimuli-affect perception, and does this occur automatically or can it be actively controlled by top-down factors? Some of the controversy in the literature stems from confounding use of terminology...
December 6, 2017: Neuroscience and Biobehavioral Reviews
https://www.readbyqxmd.com/read/29223712/correlated-colour-temperature-of-morning-light-influences-alertness-and-body-temperature
#10
Marije Te Kulve, Luc Schlangen, Lisje Schellen, Jan L Souman, Wouter van Marken Lichtenbelt
Though several studies have reported human alertness to be affected by the intensity and spectral composition of ambient light, the mechanism behind this effect is still largely unclear, especially for daytime exposure. Alerting effects of nocturnal light exposure are correlated with melatonin suppression, but melatonin levels are generally low during the day. The aim of this study was to explore the alerting effect of light in the morning for different correlated colour temperature (CCT) values, as well as its interaction with ambient temperature...
December 6, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29223640/dentin-sialophosphoprotein-expression-in-enamel-is-regulated-by-copine-7-a-preameloblast-derived-factor
#11
Su-Jin Park, Hye-Kyung Lee, You-Mi Seo, Chul Son, Hyun Sook Bae, Joo-Cheol Park
OBJECTIVE: Dentin sialophosphoprotein (Dspp) is expressed in odontoblasts and transiently expressed in early ameloblasts. However, the origin of Dspp in ameloblasts remains unclear. Our previous studies demonstrated that copine-7 (CPNE7), a molecule that is secreted by the dental epithelium, is expressed in early ameloblasts and is then translocated to differentiating odontoblasts; its expression levels correlate with odontoblast differentiation under the control of Dspp expression. The objective of this study is to figure out the relationship between CPNE7 and Dspp during amelogenesis...
December 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29223619/differential-effects-of-hepatic-cirrhosis-on-the-intrinsic-clearances-of-sorafenib-and-imatinib-by-cyps-in-human-liver
#12
Michael Murray, Tina B Gillani, Sussan Ghassabian, Robert J Edwards, Tristan Rawling
The tyrosine kinase inhibitors sorafenib and imatinib are important in the treatment of a range of cancers but adverse effects in some patients necessitate dosage modifications. CYP3A4 has a major role in the oxidation of sorafenib to its N-oxide and N-hydroxymethyl metabolites and also acts in concert with CYP2C8 to mediate imatinib N-demethylation. CYP3A4 expression and function are impaired in patients with advanced liver disease, whereas the functions of CYP2C enzymes are relatively preserved. We evaluated the biotransformation of sorafenib and imatinib in well-characterized microsomal fractions from 17 control subjects and 19 individuals with hepatic cirrhosis of varying severity...
December 6, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29223534/systemic-recovery-and-therapeutic-effects-of-transplanted-allogenic-and-xenogenic-mesenchymal-stromal-cells-in-a-rat-blunt-chest-trauma-model
#13
Elisa Maria Amann, Markus Thomas Rojewski, Sinja Rodi, Daniel Fürst, Jörg Fiedler, Annette Palmer, Sonja Braumüller, Markus Huber-Lang, Hubert Schrezenmeier, Rolf Erwin Brenner
BACKGROUND: Effective therapy of Acute Lung Injury (ALI) is still a major scientific and clinical problem. To define novel therapeutic strategies for sequelae of blunt chest trauma (TxT) like ALI/Acute Respiratory Distress Syndrome, we have investigated the immunomodulatory and regenerative effects of a single dose of ex vivo expanded human or rat mesenchymal stromal cells (hMSCs/rMSCs) with or without priming, immediately after the induction of TxT in Wistar rats. METHODS: We analyzed the histological score of lung injury, the cell count of the broncho alveolar lavage fluid (BAL), the change in local and systemic cytokine level and the recovery of the administered cells 24 h and 5 days post trauma...
December 6, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29223514/need-for-integrative-thinking-to-fight-against-emerging-infectious-diseases-proceedings-of-the-5th-seminar-on-emerging-infectious-diseases-march-22-2016-%C3%A2-current-trends-and-proposals
#14
C Burdet, J-F Guégan, X Duval, M Le Tyrant, H Bergeron, J-C Manuguerra, J Raude, C Leport, P Zylberman
We present here the proceedings of the 5th seminar on emerging infectious diseases, held in Paris on March 22nd, 2016, with seven priority proposals that can be outlined as follows: encourage research on the prediction, screening and early detection of new risks of infection; develop research and surveillance concerning transmission of pathogens between animals and humans, with their reinforcement in particular in intertropical areas ("hot-spots") via public support; pursue aid development and support in these areas of prevention and training for local health personnel, and foster risk awareness in the population; ensure adapted patient care in order to promote adherence to treatment and to epidemic propagation reduction measures; develop greater awareness and better education among politicians and healthcare providers, in order to ensure more adapted response to new types of crises; modify the logic of governance, drawing from all available modes of communication and incorporating new information-sharing tools; develop economic research on the fight against emerging infectious diseases, taking into account specific driving factors in order to create a balance between preventive and curative approaches...
December 6, 2017: Revue D'épidémiologie et de Santé Publique
https://www.readbyqxmd.com/read/29223481/can-breast-cancer-patients-with-her2-dual-equivocal-tumours-be-managed-as-her2-negative-disease
#15
Yiwei Tong, Xiaosong Chen, Xiaochun Fei, Lin Lin, Jiayi Wu, Ou Huang, Jianrong He, Li Zhu, Weiguo Chen, Yafen Li, Kunwei Shen
BACKGROUND: Increasing human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC)/fluorescence in situ hybridisation (FISH) dual-equivocal breast tumours are reported after the 2013 American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guideline update. The aim of this study is to investigate the clinico-pathologic characteristics, treatment patterns and disease outcome of these patients with HER2 dual-equivocal tumours. PATIENTS AND METHODS: Patients with HER2 IHC 2+ and available FISH results were retrospectively analysed from the Comprehensive Breast Health Center, Shanghai Ruijin Hospital...
December 7, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/29223465/biomedical-applications-of-genome-scale-metabolic-network-reconstructions-of-human-pathogens
#16
REVIEW
Laura J Dunphy, Jason A Papin
The growing global threat of antibiotic resistant human pathogens has coincided with improved methods for developing and using genome-scale metabolic network reconstructions. Consequently, there has been an increase in the number of high-quality reconstructions of relevant human and zoonotic pathogens. Novel biomedical applications of pathogen reconstructions focus on three key aspects of pathogen behavior: the evolution of antibiotic resistance, virulence factor production, and host-pathogen interactions. New methods using these reconstructions aim to improve understanding of microbe pathogenicity and guide the development of new therapeutic strategies...
December 7, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29223462/social-identity-and-cooperation-in-cultural-evolution
#17
REVIEW
Paul E Smaldino
I discuss the function of social identity signaling in facilitating cooperative group formation, and how the nature of that function changes with the structure of social organization. I propose that signals of social identity facilitate assortment for successful coordination in large-scale societies, and that the multidimensional, context-dependent nature of social identity is crucial for successful coordination when individuals have to cooperate in different contexts. Furthermore, the structure of social identity is tied to the structure of society, so that as societies grow larger and more interconnected, the landscape of social identities grows more heterogeneous...
December 6, 2017: Behavioural Processes
https://www.readbyqxmd.com/read/29223399/ectopic-serotonin-production-in-%C3%AE-cell-specific-transgenic-mice
#18
Hyeongseok Kim, Hyunki Kim, Kyuho Kim, Michael S German, Hail Kim
Genetically modified mice have been widely used in the field of β-cell research. However, analysis of results gathered using genetically modified organisms should be interpreted carefully as the results may be confounded by several factors. Here, we showed the ectopic serotonin (5-HT) production in β-cells of RIP-CreMgn, MIP-GFP, and MIP-Cre/ERT. These mice contained a human growth hormone (hGH) cassette to enhance transgene expression and hGH expression and Stat5 phosphorylation were detected in pancreatic islets of these mice...
December 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29223202/evidence-of-chaos-in-a-routine-watchstanding-task
#19
David Kern, Waldemar Karwowski, Edgar Gutiérrez Franco, Atsuo Murata
Research into human performance on naval ships often emphasizes the significance of shaping factors, such as vigilance, fatigue and circadian rhythm. However, few report on the presence of complex nonlinear dynamics and fractal dimensionality. This study examines over 7000 routine topside security checks on a U.S. Navy Destroyer performed over a twelve-month period. Time series analysis (0-1 test, analysis of Lyapunov exponent, state space plots, and graphical analysis) reveal the presence of chaotic behavior, harmonics attractors, and fractal structures...
January 2018: Nonlinear Dynamics, Psychology, and Life Sciences
https://www.readbyqxmd.com/read/29223073/evidence-for-pain-attenuation-by-the-motor-system-based-judgment-of-agency
#20
N Karsh, O Goldstein, B Eitam
Pain is an integral part of our lives. Although the effect of 'control' on sensed pain has been extensively studied and discussed, recent findings seem to be at odds with the substantial evidence for a robust motor-based sensory attenuation effect - an indirect marker for one's sense of agency. The goal of the current study was to re-examine whether there is evidence for such an effect in the context of pain. In three experiments, human participants were aversively stimulated and the sensitivity of self-reported pain to factors previously shown to modulate the sensory attenuation effect was tested (control over parameters of the stimulation; temporal contiguity and predictability, and stimulation intensity)...
December 6, 2017: Consciousness and Cognition
keyword
keyword
443
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"