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https://www.readbyqxmd.com/read/28344452/the-effects-of-a-sports-nutrition-education-intervention-on-nutritional-status-sport-nutrition-knowledge-body-composition-and-performance-during-off-season-training-in-ncaa-division-i-baseball-players
#1
Fabrício Eduardo Rossi, Andrew Landreth, Stacey Beam, Taylor Jones, Layne Norton, Jason Michael Cholewa
This study investigated the effects of a sport nutrition education intervention (SNEI) on dietary intake, knowledge, body composition, and performance in NCAA Division I baseball players. Resistance trained NCAA Division I baseball players (82.4 ± 8.2 kg; 1.83 ± 0.06 m; 13.7 ± 5 % body fat) participated in the study during 12 weeks of off-season training. Fifteen players volunteered for SNEI while 15 players matched for position served as controls (C) for body composition and performance. The nutrition intervention group (NI) received a 90 min SNEI encompassing energy intake (Kcal), carbohydrate (CHO), protein (PRO), fat, food sources, and hydration...
March 2017: Journal of Sports Science & Medicine
https://www.readbyqxmd.com/read/28337836/plant-transcription-factors-from-the-homeodomain-leucine-zipper-family-i-role-in-development-and-stress-responses
#2
REVIEW
María Florencia Perotti, Pamela Anahí Ribone, Raquel Lía Chan
In front of stressful conditions plants display adaptation mechanisms leading to changes in their morphology, physiology, development and molecular composition. Transcription factors (TFs) play crucial roles in these complex adaptation processes. This work is focused in the homeodomain-leucine zipper I (HD-Zip I) family of TFs, unique to plants. First discovered in 1991, they were identified and isolated from monocotyledonous and dicotyledonous plants showing high structural similarity and diversified functions...
March 24, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28337277/effect-and-mechanism-of-shed-on-ulcer-wound-healing-in-sprague-dawley-rat-models-with-diabetic-ulcer
#3
Yue Lv, Lihong Ge, Yuming Zhao
To evaluate the effect of stem cells from human exfoliated deciduous teeth (SHED) upon the ulcer wound healing and evaluate the mechanism underlying the role of SHED in Sprague-Dawley rat models with diabetic foot ulcer. The rats with diabetic ulcer were established and treated with SHED, mesenchymal stem cell (MSC) and PBS, respectively. The expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), matrix metalloproteinase-2 (MMP-2) and MMP9 at both protein and RNA levels was quantitatively measured...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28334006/assessing-various-infrared-ir-microscopic-imaging-techniques-for-post-mortem-interval-evaluation-of-human-skeletal-remains
#4
Claudia Woess, Seraphin Hubert Unterberger, Clemens Roider, Monika Ritsch-Marte, Nadin Pemberger, Jan Cemper-Kiesslich, Petra Hatzer-Grubwieser, Walther Parson, Johannes Dominikus Pallua
Due to the influence of many environmental processes, a precise determination of the post-mortem interval (PMI) of skeletal remains is known to be very complicated. Although methods for the investigation of the PMI exist, there still remains much room for improvement. In this study the applicability of infrared (IR) microscopic imaging techniques such as reflection-, ATR- and Raman- microscopic imaging for the estimation of the PMI of human skeletal remains was tested. PMI specific features were identified and visualized by overlaying IR imaging data with morphological tissue structures obtained using light microscopy to differentiate between forensic and archaeological bone samples...
2017: PloS One
https://www.readbyqxmd.com/read/28330244/elucidation-of-impact-of-heavy-metal-pollution-on-soil-bacterial-growth-and-extracellular-polymeric-substances-flexibility
#5
Muniswamy David, Paidi Murali Krishna, Jeybalan Sangeetha
Metal bioaccessibility is an alarming issue in croplands of mining sites due to overloading of toxic metals. Hence, the present study is aimed to determine the overloading of toxic metal in croplands across the Tawag village, Hutti, Raichur, India. Correspondingly, to identify the soil bacterial growth, physiological oxidative stress enzyme activity and surface macromolecular functional group evolution were analysed in and around the toxic metal contaminated sites through FT-IR and FT-Raman spectrometry. The evaluated results attribute that the study area is heavily polluted with the toxic metals such as arsenic, cadmium, chromium, lead and zinc...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#6
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327425/protein-drug-nanoconjugates-finding-the-alternative-proteins-as-drug-carrier
#7
Iqra Munir, Sadia Ajmal, Muhammad Raza Shah, Aftab Ahmad, Abdul Hameed, Syed Abid Ali
Present study was conducted to establish the interaction of bovine fetuin-A to validate its binding modalities with doxorubicin (Dox). Fetuin-A was purified to highest purity and monodispersity. Green synthesis of fetuin-A conjugated gold nanoparticles (F-GNPs) has been performed giving typical UV-maxima with subtle variation in fourier transform infrared spectroscopy (FTIR). Atomic force microscopy (AFM) revealed spherical shaped, polydisperse F-GNPs of varying sizes, complementing the radius of hydration (19...
March 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28315446/targeted-delivery-of-morin-a-dietary-bioflavanol-encapsulated-mannosylated-liposomes-to-the-macrophages-of-adjuvant-induced-arthritis-rats-inhibits-inflammatory-immune-response-and-osteoclastogenesis
#8
Farhath Sultana, Manoj Kumar Neog, MahaboobKhan Rasool
The purpose of the study was to develop a liposomal drug delivery system for morin, a dietary polyphenol, in order to target the synovial macrophages and investigate the remission of disease severity in the adjuvant-induced arthritic (AIA) rats. To do so, mannose decorated liposomal morin (ML-Morin) was prepared using the thin film hydration method and the physicochemical properties were characterized. The particle size and zeta potential of liposomal morin (L-Morin) was found to be 127.9nm ± 2.6 and -24.5mV ± 0...
March 14, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28301293/signatures-of-dna-target-selectivity-by-ets-transcription-factors
#9
Gregory M K Poon, Hye Mi Kim
The ETS family of transcription factors is a functionally heterogeneous group of gene regulators that share a structurally conserved, eponymous DNA-binding domain. DNA target specificity derives from combinatorial interactions with other proteins as well as intrinsic heterogeneity among ETS domains. Emerging evidence suggests molecular hydration as a fundamental feature that defines the intrinsic heterogeneity in DNA target selection and susceptibility to epigenetic DNA modification. This perspective invokes novel hypotheses in the regulation of ETS proteins in physiologic osmotic stress, their pioneering potential in heterochromatin, and the effects of passive and pharmacologic DNA demethylation on ETS regulation...
March 16, 2017: Transcription
https://www.readbyqxmd.com/read/28300413/photoinduced-chromophore-hydration-in-the-fluorescent-protein-dreiklang-is-triggered-by-ultrafast-excited-state-proton-transfer-coupled-to-a-low-frequency-vibration
#10
Fabien Lacombat, Pascal Plaza, Marie-Aude Plamont, Agathe Espagne
Because of growing applications in advanced fluorescence imaging, the mechanisms and dynamics of photoinduced reactions in reversibly photoswitchable fluorescent proteins are currently attracting much interest. We report the first time-resolved study of the photoswitching of Dreiklang, so far the only fluorescent protein to undergo reversible photoinduced chromophore hydration. Using broadband femtosecond transient absorption spectroscopy, we show that the reaction is triggered by an ultrafast deprotonation of the chromophore phenol group in the excited state in 100 fs...
March 20, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28300267/protein-conformation-in-pure-and-hydrated-deep-eutectic-solvents
#11
A Sanchez-Fernandez, K J Edler, T Arnold, D Alba Venero, A J Jackson
Deep eutectic solvents (DES) have recently been postulated as possible environments where protein structure may be preserved in the absence of water. Here we present our results towards understanding protein conformation in choline chloride-based DES and mixtures with water. Lysozyme and bovine serum albumin have been investigated by means of circular dichroism and small-angle neutron scattering.
March 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28299836/involvement-of-pka-signalling-in-anti-inflammatory-effects-of-chitosan-oligosaccharides-in-ipec-j2-porcine-epithelial-cells
#12
J W Yang, G Tian, D W Chen, Y Yao, J He, P Zheng, X B Mao, J Yu, Z Q Huang, B Yu
Weaning is characterized by intestinal inflammation, which is a big challenge in pig industry. Control of intestinal inflammation is important for improvement of growth performance and health. Therefore, the study was focused on the anti-inflammatory activity of low-molecular-weight chitosan oligosaccharide (LCOS) in a porcine small intestinal epithelial cell line (IPEC-J2). The results showed that TNF-α, as inflammation inducer, significantly upregulated the mRNA expression of IL-8 and MCP-1. Afterwards, LCOS significantly attenuated mRNA expression of IL-8 and MCP-1 induced by TNF-α in the cells...
March 16, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28297647/solvent-slaved-dynamic-processes-observed-by-tryptophan-phosphorescence-of-human-serum-albumin
#13
Andrew R Draganski, Joel M Friedman, Richard D Ludescher
Despite extensive experimental and computational efforts to understand the nature of the hierarchy of protein fluctuations and the modulating role of the protein hydration shell, a detailed microscopic description of the dynamics of the protein-solvent system has yet to be achieved. By using single tryptophan protein phosphorescence, we follow site-specific internal protein dynamics over a broad temperature range and demonstrate three independent dynamic processes. Process I is seen at temperatures below the bulk solvent Tg, has low activation energy, and is likely due to fast vibrations that may be enabled by water mobility on the protein surface...
March 14, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28296403/distinct-roles-for-interfacial-hydration-in-site-specific-dna-recognition-by-ets-family-transcription-factors
#14
Suela Xhani, Shingo Esaki, Kenneth Huang, Noa Erlitzki, Gregory M K Poon
The ETS family of transcription factors is a functionally heterogeneous group of gene regulators that share a structurally conserved, eponymous DNA-binding domain. Unlike other ETS homologs, such as Ets-1, DNA recognition by PU.1 is highly sensitive to its osmotic environment due to excess interfacial hydration in the complex. To interrogate interfacial hydration in the two homologs, we mutated a highly conserved tyrosine residue, which is exclusively engaged in coordinating a well-defined water contact between the protein and DNA among ETS proteins, to phenylalanine...
March 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28294607/molecular-environment-modulates-conformational-differences-between-crystal-and-solution-states-of-human-%C3%AE-defensin-2
#15
Jianguo Li, Zhongqiao Hu, Roger Beuerman, Chandra Verma
Human β-defensin 2 is a cysteine-rich antimicrobial peptide. In the crystal state, the N-terminal segment (residues 1-11) exhibits a helical conformation. However, a truncated form, with four amino acids removed from the N-terminus, adopts nonhelical conformations in solution, as shown by NMR. To explore the molecular origins of these different conformations, we performed Hamiltonian replica exchange molecular dynamics simulations of the peptide in solution and in the crystal state. It is found that backbone hydration and specific protein-protein interactions are key parameters that determine the peptide conformation...
March 27, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28287700/price-for-opening-the-transient-specificity-pocket-in-human-aldose-reductase-upon-ligand-binding-structural-thermodynamic-kinetic-and-computational-analysis
#16
Chris Rechlin, Frithjof Scheer, Felix Terwesten, Tobias Wulsdorf, Ewa Pol, Veronica Fridh, Philipp Toth, Wibke E Diederich, Andreas Heine, Gerhard Klebe
Insights into the thermodynamic and kinetic signature of the transient opening of a protein-binding pocket are presented resulting from accommodation of suitable substituents attached to a given parent ligand scaffold. As target, we selected human aldose reductase, an enzyme involved in development of late-stage diabetic complications. To recognize a large scope of substrate molecules, this reductase opens a transient specificity pocket. The pocket-opening step was studied by X-ray crystallography, microcalorimetry and surface plasmon resonance using a narrow series of 2-carbamoyl-phenoxy-acetic acid derivatives...
March 13, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28282987/lateral-pressure-profile-and-free-volume-properties-in-phospholipid-membranes-containing-anesthetics
#17
Balázs Fábián, Marcello Sega, Vladimir P Voloshin, Nikolai N Medvedev, Pál Jedlovszky
The effect of four general anesthetics, namely chloroform, halothane, diethyl ether, and enflurane on the free volume fraction and lateral pressure profiles in a fully hydrated dipalmitoylphosphatidylcholime (DPPC) membrane is investigated by means of computer simulation. In order to find changes that can be related to the molecular mechanism of anesthesia as well as its pressure reversal, the simulations are performed both at atmospheric and high (1000 bar) pressures. The obtained results show that the additional free volume occurring in the membrane is localized around the anesthetic molecules themselves...
March 27, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28282142/interplay-between-conformational-heterogeneity-and-hydration-in-the-folding-landscape-of-a-designed-three-helix-bundle
#18
Gregory S Custer, Payel Das, Silvina Matysiak
Water is known to play a critical role in protein folding and stability. Here we develop and employ a coarse-grained (CG) model to directly explore the role of water in shaping the conformational landscape explored during protein folding. For this purpose, we simulate a designed sequence with binary patterning of neutral and hydrophobic residues, which is capable of folding to a three-helix bundle in explicit water. We find two folded states of this sequence, with rotation of the helices occurring to trade between hydrophobic packing and water expulsion from the core...
March 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28280815/lyotropic-liquid-crystal-engineering-moving-beyond-binary-compositional-space-ordered-nanostructured-amphiphile-self-assembly-materials-by-design
#19
REVIEW
Leonie van 't Hag, Sally L Gras, Charlotte E Conn, Calum J Drummond
Ordered amphiphile self-assembly materials with a tunable three-dimensional (3D) nanostructure are of fundamental interest, and crucial for progressing several biological and biomedical applications, including in meso membrane protein crystallization, as drug and medical contrast agent delivery vehicles, and as biosensors and biofuel cells. In binary systems consisting of an amphiphile and a solvent, the ability to tune the 3D cubic phase nanostructure, lipid bilayer properties and the lipid mesophase is limited...
March 10, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28279921/computational-smart-polymer-design-based-on-elastin-protein-mutability
#20
Anna Tarakanova, Wenwen Huang, Anthony S Weiss, David L Kaplan, Markus J Buehler
Soluble elastin-like peptides (ELPs) can be engineered into a range of physical forms, from hydrogels and scaffolds to fibers and artificial tissues, finding numerous applications in medicine and engineering as "smart polymers". Elastin-like peptides are attractive candidates as a platform for novel biomaterial design because they exhibit a highly tunable response spectrum, with reversible phase transition capabilities. Here, we report the design of the first virtual library of elastin-like protein models using methods for enhanced sampling to study the effect of peptide chemistry, chain length, and salt concentration on the structural transitions of ELPs, exposing associated molecular mechanisms...
May 2017: Biomaterials
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