keyword
MENU ▼
Read by QxMD icon Read
search

protein hydration

keyword
https://www.readbyqxmd.com/read/28445750/effect-of-phosphorylation-on-a-human-like-osteopontin-peptide
#1
Samuel Lenton, Marco Grimaldo, Felix Roosen-Runge, Frank Schreiber, Tommy Nylander, Roger Clegg, Carl Holt, Michael Härtlein, Victoria García Sakai, Tilo Seydel, Susana C Marujo Teixeira
The last decade established that the dynamic properties of the phosphoproteome are central to function and its modulation. The temporal dimension of phosphorylation effects remains nonetheless poorly understood, particularly for intrinsically disordered proteins. Osteopontin, selected for this study due to its key role in biomineralization, is expressed in many species and tissues to play a range of distinct roles. A notable property of highly phosphorylated isoforms of osteopontin is their ability to sequester nanoclusters of calcium phosphate to form a core-shell structure, in a fluid that is supersaturated but stable...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28435965/conformational-and-hydration-properties-modulate-ice-recognition-by-type-i-antifreeze-protein-and-its-mutants
#2
Sandipan Chakraborty, Biman Jana
The mechanism of ice recognition by antifreeze protein (AFP) is a topic of recent interest. Here, using equilibrium simulations and free energy calculations, we provide structural rationale to the observed experimental anomalies on type I AFP (wfAFP isoform HPLC6) and its mutants as well as probe the molecular origin of ice recognition by them. Our results clearly demonstrate that the interplay between the conformational and hydration properties dictates the ice binding ability of type I AFP and its mutants...
April 24, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28435964/kinetics-of-co2-diffusion-in-human-carbonic-anhydrase-a-study-using-molecular-dynamics-simulations-and-the-markov-state-model
#3
Gong Chen, Xian Kong, Diannan Lu, Jianzhong Wu, Zheng Liu
Molecular dynamics (MD) simulations, in combination with the Markov-state model (MSM), were applied to probe CO2 diffusion from an aqueous solution into the active site of human carbonic anhydrase II (hCA-II), an enzyme useful for enhanced CO2 capture and utilization. The diffusion process in the hydrophobic pocket of hCA-II was illustrated in terms of a two-dimensional free-energy landscape. We found that CO2 diffusion in hCA-II is a rate-limiting step in the CO2 diffusion-binding-reaction process. The equilibrium distribution of CO2 shows its preferential accumulation within a hydrophobic domain in the protein core region...
April 24, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28430436/time-domain-thz-spectroscopy-reveals-coupled-protein-hydration-dielectric-response-in-solutions-of-native-and-fibrils-of-human-lysozyme
#4
Fabio Novelli, Saeideh Ostovar Pour, Jonathan O Tollerud, Ashkan Roozbeh, Dominique R T Appadoo, Ewan W Blanch, Jeffrey A Davis
Here we reveal details of the interaction between human lysozyme proteins, both native and fibrils, and their water environment by intense terahertz time domain spectroscopy. With the aid of a rigorous dielectric model, we determine the amplitude and phase of the oscillating dipole induced by the THz field in the volume containing the protein and its hydration water. At low concentrations, the amplitude of this induced dipolar response decreases with increasing concentration. Beyond a certain threshold, marking the onset of the interactions between the extended hydration shells, the amplitude remains fixed but the phase of the induced dipolar response, which is initially in phase with the applied THz field, begins to change...
April 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28429932/thermodynamics-of-the-thermal-denaturation-of-acid-molten-globule-state-of-cytochrome-c-indicate-a-reversible-high-temperature-oligomerization-process
#5
Shigeyoshi Nakamura, Tomonori Saotome, Akiko Nakazawa, Masao Fukuda, Yutaka Kuroda, Shun-Ichi Kidokoro
In this study, we performed differential scanning calorimetry (DSC) and pressure perturbation calorimetry (PPC) analysis of the thermal transition of cytochrome c from an acidic molten globule (MG) state with the protein concentrations of 0.5-18.2 mg/mL. DSC profiles were highly reversible and showed clear protein-concentration dependence, indicating that reversible oligomerization occurred accompanying the thermal transition from the MG state. The DSC and PPC data required at least a six-state model including three new oligomeric states: dimer (I2), trimer (I3), and tetramer (I4) in addition to the three monomeric states previously characterized...
April 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28429509/co2-permeability-and-carbonic-anhydrase-activity-of-rat-cardiomyocytes
#6
Mariela Arias-Hidalgo, Samer Al-Samir, Natalie Weber, Cornelia Geers-Knörr, Gerolf Gros, Volker Endeward
AIM: We determine here the CO2 permeability (PCO2 ) of plasma membranes of cardiomyocytes. These cells were chosen because heart possesses the highest rate of O2 -consumption/CO2 -production in the body. METHODS: Cardiomyocytes were isolated from rat hearts using the Langendorff technique. Cardiomyocyte suspensions exhibited a vitality of 2-14% and were studied by the previously described mass spectrometric (18) O-exchange-technique deriving PCO2 . We show by mass spectrometry and by carbonic anhydrase (CA)-staining that non-vital cardiomyocytes are free of CA and thus do not contribute to the mass spectrometric signal, which is determined exclusively by the fully functional vital cardiomyocytes...
April 20, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28426976/alternative-electron-transport-pathways-in-photosynthesis-a-confluence-of-regulation
#7
REVIEW
Jean Alric, Xenie Johnson
Photosynthetic reactions proceed along a linear electron transfer chain linking water oxidation at photosystem II (PSII) to CO2 reduction in the Calvin-Benson-Bassham cycle. Alternative pathways poise the electron carriers along the chain in response to changing light, temperature and CO2 inputs, under prolonged hydration stress and during development. We describe recent literature that reports the physiological functions of new molecular players. Such highlights include the flavodiiron proteins and their important role in the green lineage...
April 17, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28424587/interactions-of-the-mechanosensitive-channels-with-extracellular-matrix-integrins-and-cytoskeletal-network-in-osmosensation
#8
REVIEW
Runsheng Jiao, Dan Cui, Stephani C Wang, Dongyang Li, Yu-Feng Wang
Life is maintained in a sea water-like internal environment. The homeostasis of this environment is dependent on osmosensory system translation of hydromineral information into osmotic regulatory machinery at system, tissue and cell levels. In the osmosensation, hydromineral information can be converted into cellular reactions through osmoreceptors, which changes thirst and drinking, secretion of antidiuretic vasopressin (VP), reabsorption of water and salt in the kidneys at systemic level as well as cellular metabolic activity and survival status at tissue level...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28421659/the-anti-inflammatory-effects-of-morin-hydrate-in-atherosclerosis-is-associated-with-autophagy-induction-through-camp-signaling
#9
Yue Zhou, Zhan-Qi Cao, Hong-Yuan Wang, Yan-Na Cheng, Lu-Gang Yu, Xin-Ke Zhang, Yan Sun, Xiu-Li Guo
SCOPE: Although the previous trials of inflammation have indicated that morin hydrate (MO) hold considerable promise, understanding the distinct mechanism of MO against inflammation remains a challenge. METHODS AND RESULTS: This study investigated the effect of MO in atherosclerosis in ApoE(-/-) mice and underlying cell signaling of MO effect in inflammation in human umbilical vein endothelial cells (HUVECs). Administration of MO significantly reduced serum lipid level, inflammatory cytokines (TNF-α and ICAM-1) and atherosclerotic plaque formation in vivo...
April 18, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28418707/liquid-water-restricts-habitability-in-extreme-deserts
#10
Charles S Cockell, Sarah Brown, Hanna Landenmark, Toby Samuels, Rebecca Siddall, Jennifer Wadsworth
Liquid water is a requirement for biochemistry, yet under some circumstances it is deleterious to life. Here, we show that liquid water reduces the upper temperature survival limit for two extremophilic photosynthetic microorganisms (Gloeocapsa and Chroococcidiopsis spp.) by greater than 40°C under hydrated conditions compared to desiccated conditions. Under hydrated conditions, thermal stress causes protein inactivation as shown by the fluorescein diacetate assay. The presence of water was also found to enhance the deleterious effects of freeze-thaw in Chroococcidiopsis sp...
April 2017: Astrobiology
https://www.readbyqxmd.com/read/28415454/development-of-a-dna-liposome-complex-for-gene-delivery-applications
#11
M Rasoulianboroujeni, G Kupgan, F Moghadam, M Tahriri, A Boughdachi, P Khoshkenar, J J Ambrose, N Kiaie, D Vashaee, J D Ramsey, L Tayebi
The association structures formed by cationic liposomes and DNA (Deoxyribonucleic acid)-liposome have been effectively utilized as gene carriers in transfection assays. In this research study, cationic liposomes were prepared using a modified lipid film hydration method consisting of a lyophilization step for gene delivery applications. The obtained results demonstrated that the mean particle size had no significant change while the polydispersity (PDI) increased after lyophilization. The mean particle size slightly reduced after lyophilization (520±12nm to 464±25nm) while the PDI increased after lyophilization (0...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414445/preferential-solvation-hydration-of-%C3%AE-chymotrypsin-in-water-acetonitrile-mixtures
#12
Vladimir A Sirotkin, Alexandra A Kuchierskaya
The aim of our study is to monitor the preferential hydration/solvation of the protein macromolecules at low and high water content in water-organic mixtures. Our approach is based on the analysis of the absolute values of the water/organic solvent sorption. We applied this approach to estimate the protein stabilization/destabilization due to the preferential interactions of α-chymotrypsin with water-acetonitrile mixtures. At high water content, α-chymotrypsin is preferentially hydrated. At the intermediate water content, the preferential interaction changed from preferential hydration to preferential binding of acetonitrile...
April 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28409932/study-of-protein-dynamics-under-nanoconfinement-by-spin-label-esr-a-case-of-t4-lysozyme-protein
#13
Kuo-Jung Chang, Yun-Hsuan Kuo, Yun-Wei Chiang
The electron spin resonance (ESR) spectra of spin-labeled proteins are sensitive to dynamics, but discrimination between the various dynamics is often difficult. Here, we report an improvement in ESR spectral sensitivity to local backbone dynamics of a protein by a methodology that performs ESR measurement when the protein is confined in the nanochannels of a mesoporous material. An extensive set of ESR data, which includes the spectra of a nitroxide-based side chain from buried and solvent-exposed sites of a T4 lysozyme (T4L) protein, were obtained over a range of temperatures, 200-300 K, to explore the dynamics of T4L under nanoconfinement...
April 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28406028/water-distribution-dynamics-and-interactions-with-alzheimer-s-%C3%AE-amyloid-fibrils-investigated-by-solid-state-nmr
#14
Tuo Wang, Hyunil Jo, William F DeGrado, Mei Hong
Water is essential for protein folding and assembly of amyloid fibrils. Internal water cavities have been proposed for several amyloid fibrils, but no direct structural and dynamical data have been reported on the water dynamics and site-specific interactions of water with the fibrils. Here we use solid-state NMR spectroscopy to investigate the water interactions of several Aβ40 fibrils. (1)H spectral lineshapes, T2 relaxation times, and two-dimensional (2D) (1)H-(13)C correlation spectra show that there are five distinct water pools: three are peptide-bound water, while two are highly dynamic water that can be assigned to interfibrillar water and bulk-like matrix water...
April 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28393138/new-method-to-study-the-vibrational-modes-of-biomolecules-in-the-terahertz-range-based-on-a-single-stage-raman-spectrometer
#15
Basanth S Kalanoor, Maria Ronen, Ziv Oren, Doron Gerber, Yaakov R Tischler
The low-frequency vibrational (LFV) modes of biomolecules reflect specific intramolecular and intermolecular thermally induced fluctuations that are driven by external perturbations, such as ligand binding, protein interaction, electron transfer, and enzymatic activity. Large efforts have been invested over the years to develop methods to access the LFV modes due to their importance in the studies of the mechanisms and biological functions of biomolecules. Here, we present a method to measure the LFV modes of biomolecules based on Raman spectroscopy that combines volume holographic filters with a single-stage spectrometer, to obtain high signal-to-noise-ratio spectra in short acquisition times...
March 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/28391697/synthesis-and-thrombogenicity-evaluation-of-poly-3-methoxypropionic-acid-vinyl-ester-a-candidate-for-blood-compatible-polymer
#16
Kazuhiro Sato, Shingo Kobayashi, Asuka Sekishita, Miyuki Wakui, Masaru Tanaka
A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple 2-step reaction, and then, the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the non-thrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and ELISA...
April 10, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28390725/comparison-of-a-commercially-available-oral-nutritional-supplement-and-intravenous-fluid-therapy-for-dehydration-in-dairy-calves
#17
Jared D Taylor, Merel Rodenburg, Timothy A Snider
Calf scours is a primary cause of morbidity and mortality in the dairy industry. Effective treatments are needed to minimize death, maximize welfare, and maintain growth and productivity. The objective of this trial was to compare the efficacy of a commercially available nutritional supplement (Diaque, Boehringer-Ingelheim Vetmedica Inc., St. Joseph, MO) and i.v. lactated Ringer's solution (LRS) in rehydrating, preventing acidemia, and correcting electrolyte imbalances in an experimental model for calf scours...
April 5, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28388167/collective-hydration-dynamics-in-some-amino-acid-solutions-a-combined-ghz-thz-spectroscopic-study
#18
Nirnay Samanta, Debasish Das Mahanta, Samiran Choudhury, Anjan Barman, Rajib Kumar Mitra
A detailed understanding of hydration of amino acids, the building units of protein, is a key step to realize the overall solvation processes in proteins. In the present contribution, we have made a combined GHz (0.2-50) to THz (0.3-2.0) experimental spectroscopic study to investigate the dynamics of water at room temperature in the presence of different amino acids (glycine, L-serine, L-lysine, L-tryptophan, L-arginine, and L-aspartic acid). The THz absorption coefficient, α(ν), of amino acids follows a trend defined by their solvent accessible surface area...
March 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28386087/delivery-of-enac-sirna-to-epithelial-cells-mediated-by-a-targeted-nanocomplex-a-therapeutic-strategy-for-cystic-fibrosis
#19
Maria D I Manunta, Aristides D Tagalakis, Martin Attwood, Ahmad M Aldossary, Josephine L Barnes, Mustafa M Munye, Alexander Weng, Robin J McAnulty, Stephen L Hart
The inhibition of ENaC may have therapeutic potential in CF airways by reducing sodium hyperabsorption, restoring lung epithelial surface fluid levels, airway hydration and mucociliary function. The challenge has been to deliver siRNA to the lung with sufficient efficacy for a sustained therapeutic effect. We have developed a self-assembling nanocomplex formulation for siRNA delivery to the airways that consists of a liposome (DOTMA/DOPE; L), an epithelial targeting peptide (P) and siRNA (R). LPR formulations were assessed for their ability to silence expression of the transcript of the gene encoding the α-subunit of the sodium channel ENaC in cell lines and primary epithelial cells, in submerged cultures or grown in air-liquid interface conditions...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28384968/paraneoplastic-hypoglycaemia-a-rare-manifestation-of-pelvic-gastrointestinal-stromal-tumour
#20
Ashish Singhal, Rahat Hadi, Kiranpreet Mehrotra, Shivani Rastogi, Shakeel Masood
Non-Islet Cell Tumour Induced Hypoglycaemia (NICTH), presenting with recurrent fasting hypoglycaemia is a very rare paraneoplastic syndrome. It usually presents with large metastatic mesenchymal tumours. NICTH secondary to Gastrointestinal Stromal Tumour (GIST) is even rarer. Diagnosis of NICTH is based on the low serum insulin level, low serum concentrations of Insulin Like Growth Factor (IGF-I) and IGF binding protein- III (IGFBP-III) in combination with elevated concentrations of pro-IGF-II. Various Immunohistochemical (IHC) markers are integral to diagnosis of GIST namely 2-deoxyglucose-6-phosphate phosphatase -1(DOG-1), Cluster Differentiation 34 (CD 34), Cluster Differentiation 117 (CD117)...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
keyword
keyword
44298
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"