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https://www.readbyqxmd.com/read/29465051/resistance-pattern-and-detection-of-metallo-beta-lactamase-genes-in-clinical-isolates-of-pseudomonas-aeruginosa-in-a-central-nigeria-tertiary-hospital
#1
K O Zubair, K C Iregbu
Background: Acquired metallo-β-lactamases (MBLs) pose serious problem both in terms of treatment and infection control in the hospitals and report across the world showed an increase in their prevalence. However, there is a paucity of data from Africa, and their report is rare in Nigeria. Aim: This study aimed to determine the prevalence of acquired MBL-resistant genes in carbapenem-resistant Pseudomonas aeruginosa in Abuja, North Central Nigeria. Materials and Methods: Two hundred nonduplicate, consecutive isolates of P...
February 2018: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29464424/prevalence-and-risk-factors-for-ctx-m-gram-negative-bacteria-in-hospitalized-patients-at-a-tertiary-care-hospital-in-kilimanjaro-tanzania
#2
Tolbert Sonda, Happiness Kumburu, Marco van Zwetselaar, Michael Alifrangis, Blandina T Mmbaga, Ole Lund, Frank M Aarestrup, Gibson Kibiki
Emergence and spread of extended spectrum beta-lactamase (ESBL)-producing gram-negative bacteria, mainly due to CTX-M, is a major global public health problem. Patients infected with ESBL-producing gram-negative bacteria have an increased risk of treatment failure and death. We investigated the prevalence and risk factors for CTX-M gram-negative bacteria isolated from clinical specimens of patients hospitalized at a tertiary care hospital in Kilimanjaro, Tanzania. Isolated gram-negative bacteria from inpatients admitted at Kilimanjaro Christian Medical Centre (KCMC) between August 2013 and August 2015 were fully genome sequenced...
February 20, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29463684/strategy-to-reduce-e-coli-bacteraemia-based-on-cohort-data-from-a-london-teaching-hospital
#3
Desmond Hsu, Mark Melzer
BACKGROUND AND AIM: In 2017, National Health Service Improvement set a 10% reduction target for Escherichia coli bacteraemia by 2018, followed by a 50% reduction in healthcare-associated Gram-negative bacteraemias by 2022. We analysed consecutive cases of E. coli bacteraemia and devised a strategy to achieve these targets. METHODS: From December 2012 to November 2013, demographic, clinical and microbiological data were prospectively collected on all patients with bacteraemia at the Royal London Hospital in East London, UK...
February 20, 2018: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/29461928/comparison-of-clinical-isolates-of-aeromonas-from-singapore-and-malaysia-with-regard-to-molecular-identification-virulence-and-antimicrobial-profiles
#4
Wei Ching Khor, Suat Moi Puah, Tse Hsien Koh, Jin Ai Mary Anne Tan, Savithiri D Puthucheary, Kek Heng Chua
OBJECTIVE: The objective of this study was to examine the species distribution, genetic relatedness, virulence gene profiles, antimicrobial sensitivities, and resistance gene distribution of clinical Aeromonas strains from Singapore and Malaysia. METHODS: A total of 210 Aeromonas clinical isolates were investigated: 116 from Singapore General Hospital and 94 archived clinical isolates from University of Malaya Medical Center, Malaysia. The isolates were genetically identified based on the gcat gene screening and the partial sequences of the rpoD housekeeping gene...
February 20, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29461808/tio-2-assisted-laser-desorption-ionization-mass-spectrometry-for-rapid-profiling-of-candidate-metabolite-biomarkers-from-antimicrobial-resistant-bacteria
#5
Rutan Zhang, Qin Qin, Baohong Liu, Liang Qiao
Antimicrobial resistance (AMR) is one of the most serious problems affecting public health and safety. It is crucial to understand antimicrobial resistance from molecular level. In this work, TiO2 assisted laser desorption/ionization (LDI) mass spectrometry (MS) was used for fast metabolites analysis from intact bacterial cells to discriminate different strains of bacteria and to detect AMR. With the mass spectra of bacterial metabolites by TiO2 -LDI MS, multivariable analysis was performed for bacterial identification to find out distinctive metabolites as potential biomarkers...
February 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29459649/evolving-bacterial-fitness-with-an-expanded-genetic-code
#6
Drew S Tack, Austin C Cole, Raghav Shroff, Barrett R Morrow, Andrew D Ellington
Since the fixation of the genetic code, evolution has largely been confined to 20 proteinogenic amino acids. The development of orthogonal translation systems that allow for the codon-specific incorporation of noncanonical amino acids may provide a means to expand the code, but these translation systems cannot be simply superimposed on cells that have spent billions of years optimizing their genomes with the canonical code. We have therefore carried out directed evolution experiments with an orthogonal translation system that inserts 3-nitro-L-tyrosine across from amber codons, creating a 21 amino acid genetic code in which the amber stop codon ambiguously encodes either 3-nitro-L-tyrosine or stop...
February 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29459597/prevalence-of-metallo-%C3%AE-lactamase-producing-non-fermentative-pseudomonas-species-from-clinical-isolates-in-dhaka-bangladesh
#7
M N Islam, D Ahmed, M A Hossain, C R Ahsan, M Yasmin
Antimicrobial drug resistance, a global concern, has been increasing unpredictably in microorganism causing human infections specially among Gram negative non-fermenting Pseudomonas spp. Carbapenems, a beta lactam antibiotics, are the most potent and effective drug usually kept reserved for treating the multi-drug resistant Psedomonas spp and other infections caused by organisms producing Extended Spectrum Beta Lactamase (ESBL) and AmpC. Clinical utility of carbapenem will reduce when resistant bacteria evolve due to production of carbapenem hydrolyzing Metallo-β-lactamase (MBL) which confers high-level resistance to all beta-lactam antibiotics except aztreonam...
January 2018: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/29458443/epidemiology-and-control-measures-of-an-oxa-48-producing-enterobacteriaceae-hospital-wide-oligoclonal-outbreak
#8
V Pérez-Blanco, L Redondo-Bravo, G Ruíz-Carrascoso, J R Paño-Pardo, R Gómez-Gil, A Robustillo-Rodela, J García-Rodríguez, J Mingorance, R Herruzo
The main objective of our study was to describe the epidemiological and microbiological features of an oligoclonal hospital-wide outbreak caused by OXA-48-producing Enterobacteriaceae (OXA-48-PE). OXA-48 is a carbapenemase belonging to Ambler class D beta-lactamases, identified frequently in the Mediterranean and Southern European countries, and associated with several Enterobacteriaceae species. An outbreak of OXA-48-PE with a complex epidemic pattern was detected in January 2011. Initial control measures included contact precautions and the reinforcement of infection control practices, but despite all efforts made, the epidemiological situation hardly changed and new measures were implemented during 2013...
February 20, 2018: Epidemiology and Infection
https://www.readbyqxmd.com/read/29458432/physicians-knowledge-attitudes-and-perceptions-concerning-antibiotic-resistance-a-survey-in-a-ghanaian-tertiary-care-hospital
#9
Appiah-Korang Labi, Noah Obeng-Nkrumah, Stephanie Bjerrum, Nii Armah Adu Aryee, Yaw Adjei Ofori-Adjei, Alfred E Yawson, Mercy J Newman
BACKGROUND: Understanding the knowledge, attitudes and practices of physicians towards antibiotic resistance is key to developing interventions aimed at behavior change. The survey aimed to investigate physicians' knowledge and attitudes towards antibiotic resistance in a tertiary-care hospital setting in Ghana. METHODS: We conducted a cross-sectional respondent-driven survey using a 40-item, anonymous, voluntary, traditional paper-and-pencil self-administered questionnaire among 159 physicians at Korle-Bu Teaching Hospital...
February 20, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29458104/a-new-l-glutaminase-from-streptomyces-pratensis-nrc-10-gene-identification-enzyme-purification-and-characterization
#10
Sanaa E Tork, Magda M Aly, Omar Elsemin
In the current study, the purified l-glutaminase from Streptomyces pratensis NRC10 (GenBank number KC857622) was characterized. Its molecular weight was estimated to be 46 kDa and isoelectric point 7.4. Its Vmax was calculated to be 2.19 U/mg/min, while Km was 0.175 mM. The optimum pH and temperature were 9 and 45 °C, respectively. It was thermostable at 45 °C but thermally inactivated at 60 °C after 50 min. Moreover, its enzymatic activity was enhanced by K+ ions and inhibited by Mg2+ , Cu2+ , Ag+ , Hg2+ , Ni2+ , Fe2+ , Cr2 , Na+ , Ca2+ , and EDTA...
February 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29456528/mutation-in-esbl-plasmid-from-escherichia-coli-o104-h4-leads-autoagglutination-and-enhanced-plasmid-dissemination
#11
Mickaël Poidevin, Mari Sato, Ipek Altinoglu, Manon Delaplace, Chikara Sato, Yoshiharu Yamaichi
Conjugative plasmids are one of the main driving force of wide-spreading of multidrug resistance (MDR) bacteria. They are self-transmittable via conjugation as carrying the required set of genes and cis -acting DNA locus for direct cell-to-cell transfer. IncI incompatibility plasmids are nowadays often associated with extended-spectrum beta-lactamases producing Enterobacteria in clinic and environment. pESBL-EA11 was isolated from Escherichia coli O104:H4 outbreak strain in Germany in 2011. During the previous study identifying transfer genes of pESBL-EA11, it was shown that transposon insertion at certain DNA region of the plasmid, referred to as Hft, resulted in great enhancement of transfer ability...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29455172/transmission-of-esbl-producing-enterobacteriaceae-and-their-mobile-genetic-elements-identification-of-sources-by-whole-genome-sequencing-study-protocol-for-an-observational-study-in-switzerland
#12
Tanja Stadler, Dominik Meinel, Lisandra Aguilar-Bultet, Jana S Huisman, Ruth Schindler, Adrian Egli, Helena M B Seth-Smith, Lucas Eichenberger, Peter Brodmann, Philipp Hübner, Claudia Bagutti, Sarah Tschudin-Sutter
INTRODUCTION: Extended-spectrum beta-lactamases (ESBL)-producing Enterobacteriaceae were first described in relation with hospital-acquired infections. In the 2000s, the epidemiology of ESBL-producing organisms changed as especially ESBL-producing Escherichia coli was increasingly described as an important cause of community-acquired infections, supporting the hypothesis that in more recent years ESBL-producing Enterobacteriaceae have probably been imported into hospitals rather than vice versa...
February 17, 2018: BMJ Open
https://www.readbyqxmd.com/read/29447989/aetiology-of-lower-respiratory-tract-infection-in-adults-in-primary-care-a-prospective-study-in-11-european-countries
#13
Margareta Ieven, Samuel Coenen, Katherine Loens, Christine Lammens, Frank Coenjaerts, Anouk Vanderstraeten, Birgitta Henriques-Normark, Derrick Crook, Kris Huygen, Chris C Butler, Theo Jm Verheij, Paul Little, Kalina Zlateva, Anton van Loon, Eric Cj Claas, Herman Goossens
OBJECTIVES: To describe the role of bacteria (including bacterial resistance), viruses (including those recently described), and mixed bacterial-viral infections in adults presenting to primary care with lower respiratory tract infection (LRTI). METHODS: We enrolled 3104 adults with LRTI, 141 (4.5%) of whom had community-acquired pneumonia (CAP), and 2985 matched controls in a prospective study in 16 primary care networks in Europe, and followed patients up at 28-35 days...
February 12, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29444952/treatment-of-infections-caused-by-extended-spectrum-beta-lactamase-ampc-and-carbapenemase-producing-enterobacteriaceae
#14
REVIEW
Jesús Rodríguez-Baño, Belén Gutiérrez-Gutiérrez, Isabel Machuca, Alvaro Pascual
Therapy of invasive infections due to multidrug-resistant Enterobacteriaceae (MDR-E) is challenging, and some of the few active drugs are not available in many countries. For extended-spectrum β-lactamase and AmpC producers, carbapenems are the drugs of choice, but alternatives are needed because the rate of carbapenem resistance is rising. Potential active drugs include classic and newer β-lactam-β-lactamase inhibitor combinations, cephamycins, temocillin, aminoglycosides, tigecycline, fosfomycin, and, rarely, fluoroquinolones or trimethoprim-sulfamethoxazole...
April 2018: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/29442147/-urosepsis
#15
Mathias W Pletz, Sebastian Weis, Christina Forstner, Florian Wagenlehner
Urosepsis is defined as a severe disease due to organ failure caused by a urinary tract infection. An empirical antibiotic therapy should be instigated within the first hour after diagnosis. Urine cultures and blood cultures should be performed before antibiotic treatment. Further diagnostics should be carried out at an early stage to enable an interventional focus control in the case of urinary tract obstruction or abscess formation, if necessary. Gram-negative pathogens are most frequently isolated. Extended spectrum beta-lactamase (ESBL) forming bacteria as a cause of urosepsis are increasing...
February 7, 2018: Medizinische Klinik, Intensivmedizin und Notfallmedizin
https://www.readbyqxmd.com/read/29437541/molecular-characterization-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-pseudomonas-aeruginosa-strains-from-a-malaysian-tertiary-hospital
#16
Hannah Y P Phoon, Hazilawati Hussin, Baizurah M Hussain, Kwai Lin Thong
Pseudomonas aeruginosa infections account for high morbidity and mortality rates worldwide. Increasing resistance toward β-lactams, especially carbapenems, poses a serious therapeutic challenge. However, the multilocus sequence typing (MLST) of extended-spectrum beta lactamase (ESBL)- and carbapenemase-producing clinical P. aeruginosa has not been reported in Malaysia. This study aimed to determine the antibiotic susceptibility profiles, resistance genes, pulsotypes, and sequence types (STs) of clinical P...
February 13, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29437306/clinical-predictors-of-ceftriaxone-resistance-in-microorganisms-causing-febrile-urinary-tract-infections-in-men
#17
Javier Ramos Lázaro, Alex Smithson, Neus Jovè Vidal, María Teresa Batida Vila
OBJECTIVES: Because of high rates of resistance to fluoroquinolones, ceftriaxone has become one of the main options for treating febrile urinary tract infection (FUTI). This study aimed to identify predictors of ceftriaxone resistance in community-acquired FUTIs in men. MATERIAL AND METHODS: Cross-sectional ambispective study enrolling men with FUTIs treated in the emergency department of a local area hospital in Spain. RESULTS: A total of 552 FUTI episodes were studied; 103 (18...
February 2018: Emergencias: revista de la Sociedad Española de Medicina de Emergencias
https://www.readbyqxmd.com/read/29434398/a-highly-promiscuous-integron-plasmids-extended-spectrum-beta-lactamases-and-efflux-pumps-as-factors-governing-multidrug-resistance-in-a-highly-drug-resistant-vibrio-fluvialis-isolate-bd146-from-kolkata-india
#18
Neha Rajpara, Mrinalini Nair, Ashima Kushwaha Bhardwaj
In an earlier study from this laboratory, Vibrio fluvialis BD146, a clinical isolate from Kolkata, India, 2002, was found to be resistant to all the fourteen antibiotics tested. It harboured a high copy number plasmid pBD146 and a low copy number plasmid. In the present study, a more detailed analysis was carried out to unravel different resistance mechanisms in this isolate. Sequencing showed that variable region of class 1 integron located on low copy number plasmid harbored arr3-cmlA-blaOXA10-aadA1 gene cassettes...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29433582/the-frequency-of-klebsiella-pneumonia-encoding-genes-for-ctx-m-tem-1-and-shv-1-extended-spectrum-beta-lactamases-enzymes-isolated-from-urinary-tract-infection
#19
Masomeh Dehshiri, Seyed Sajjad Khoramrooz, Mohammad Zoladl, Seyed Abdolmajid Khosravani, Najmeh Parhizgari, Mohammad Hossein Motazedian, Soheyla Jahedi, Asghar Sharifi
BACKGROUND: The extended- spectrum β-lactamase producing bacteria are widely spread worldwide. The productions of these enzymes cause bacterial resistance to a wide range of antibiotics. The aim of this study was to investigated the frequency of K. pneumonia encoding genes for CTX-M, TEM-1 and SHV-1 extended-spectrum beta lactamases enzymes isolated from urinary tract infection. METHODS: This study is cross-sectional study. All K. pneumonia isolates from urine samples, which had grown on media culture more than 105 were delivered to the medical microbiology laboratory...
February 13, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29432938/first-report-on-antimicrobial-resistance-and-molecular-characterization-of-salmonella-enterica-serotype-typhi-isolated-from-human-specimens-in-luanda-angola
#20
Moisés Francisco, Sofia Santos Costa, Adriana Belas, Jorge Ramos, Isabel Couto, Constança Pomba, Miguel Viveiros
OBJECTIVES: Typhoid fever is a common infection in Africa and in spite of scarce surveillance reports, its incidence is commonly considered high by the Angolan Health system. Drug-resistant Salmonella enterica serotype Typhi has emerged, turning antimicrobial susceptibility testing essential to provide clinical guidance. This is the first report analyzing antimicrobial resistance patterns and population structure of the few S. enterica ser. Typhi isolated from patients with Typhoid fever in Luanda, Angola...
February 9, 2018: Journal of Global Antimicrobial Resistance
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