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https://www.readbyqxmd.com/read/28487628/acute-exposure-to-fluoxetine-alters-aggressive-behavior-of-zebrafish-and-expression-of-genes-involved-in-serotonergic-system-regulation
#1
Antonia Theodoridi, Aleka Tsalafouta, Michail Pavlidis
Zebrafish, Danio rerio, is an emerging model organism in stress and neurobehavioral studies. In nature, the species forms shoals, yet when kept in pairs it exhibits an agonistic and anxiety-like behavior that leads to the establishment of dominant-subordinate relationships. Fluoxetine, a selective serotonin reuptake inhibitor, is used as an anxiolytic tool to alter aggressive behavior in several vertebrates and as an antidepressant drug in humans. Pairs of male zebrafish were held overnight to develop dominant-subordinate behavior, either treated or non-treated for 2 h with fluoxetine (5 mg L(-1)), and allowed to interact once more for 1 h...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28479267/anxiolytic-like-effects-of-noribogaine-in-zebrafish
#2
Allan V Kalueff, Aleksandra Kaluyueva, Emeline L Mailet
Noribogaine is the main psychoactive metabolite of the hallucinogenic drug ibogaine, and is a particularly interesting compound potentially useful to treat dependence and various psychiatric disorders. Here, we report the effects of noribogaine on anxiety and locomotion in zebrafish (Danio rerio), a new promising model organism in neurobehavioral and psychopharmacological research. Adult zebrafish were subjected to the 5min novel tank test (NTT) following an acute, 20min drug immersion in 1, 5 and 10mg/L noribogaine...
May 4, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28466455/valorization-of-proteins-from-co-and-by-products-from-the-fish-and-meat-industry
#3
REVIEW
Tone Aspevik, Åge Oterhals, Sissel Beate Rønning, Themis Altintzoglou, Sileshi Gizachew Wubshet, Asbjørn Gildberg, Nils Kristian Afseth, Ragnhild Dragøy Whitaker, Diana Lindberg
Large volumes of protein-rich residual raw materials, such as heads, bones, carcasses, blood, skin, viscera, hooves and feathers, are created as a result of processing of animals from fisheries, aquaculture, livestock and poultry sectors. These residuals contain proteins and other essential nutrients with potentially bioactive properties, eligible for recycling and upgrading for higher-value products, e.g. for human, pet food and feed purposes. Here, we aim to cover all the important aspects of achieving optimal utilization of proteins in such residual raw materials, identifying those eligible for human consumption as co-products and for feed applications as by-products...
June 2017: Topics in Current Chemistry (Journal)
https://www.readbyqxmd.com/read/28460686/consequences-of-uncontrolled-cooling-during-sterlet-acipenser-ruthenus-sperm-cryopreservation-on-post-thaw-motility-and-fertilizing-ability
#4
Yevhen Horokhovatskyi, Marek Rodina, Hadiseh Dadras Asyabar, Sergii Boryshpolets, Borys Dzyuba
The significant influence of the number and position of fish sperm sample straws in uncontrolled cooling devices on post-thaw spermatozoa parameters, such as motility and fertilizing ability, is presented in this study. The two most popular uncontrolled cooling devices were used in this study: a Styrofoam box setup with a polystyrene floating raft on liquid nitrogen and the dry shipper setup with a straw holder. We tested the effect of different quantities of straws (6 or 60) placed on the polystyrene floating raft and the position of the straws in the holder (on the periphery or in the centre)...
June 2017: Theriogenology
https://www.readbyqxmd.com/read/28450256/proteomic-identification-of-seminal-plasma-proteins-related-to-the-freezability-of-carp-semen
#5
Mariola A Dietrich, Ilgiz Irnazarow, Andrzej Ciereszko
The variation in sperm freezability among individuals within a fish species is a major factor justifying the identification of useful predictive indicators of cryopreservation success. It is unknown at present whether the protein composition of fish seminal plasma affects sperm freezability. Therefore, the aims of this study were to compare the proteome of carp seminal plasma from semen rated as good (GF) and poor (PF) freezability by two-dimensional difference gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry...
April 24, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28395972/optimization-of-conditions-for-the-cryopreservation-of-yellow-catfish-pelteobagrus-fulvidraco-sperm
#6
Sen Yang, Linqiang Han, Rushou Huang, Yongzhong Liufu, Zining Meng, Haoran Lin
Yellow catfish (Pelteobagrus fulvidraco) is a promising aquaculture species in China with an increasing market demand. To serve the growing demand of male broodstock for artificial fertilization and the preservation of valuable strains for selective breeding, we tried to develop a species-specific cryopreservation protocol for yellow catfish sperm in this study. Important factors such as cryoprotectant, freezing height above the liquid nitrogen (LN) surface, dilution ratio, equilibration time, thawing temperature and cool storage before freezing were standardized...
April 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28381233/structural-integrity-and-viability-of-fredericella-sultana-statoblasts-infected-with-tetracapsuloides-bryosalmonae-myxozoa-under-diverse-treatment-conditions
#7
Ahmed Abd-Elfattah, Mansour El-Matbouli, Gokhlesh Kumar
Fredericella sultana is an invertebrate host of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. The bryozoan produces seed-like statoblasts to facilitate its persistence during unfavourable conditions. Statoblasts from infected bryozoans can harbor T. bryosalmonae and give rise to infected bryozoan colonies when conditions improve. We aimed in the present study to evaluate the integrity and viability of T. bryosalmonae-infected statoblasts after a range of harsh treatment conditions...
April 5, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28361887/mechanisms-of-social-buffering-of-fear-in-zebrafish
#8
Ana I Faustino, André Tacão-Monteiro, Rui F Oliveira
Some humans thrive whereas others resign when exposed to threatening situations throughout life. Social support has been identified as an important modulator of these discrepancies in human behaviour, and other social animals also exhibit phenomena in which individuals recover better from aversive events when conspecifics are present - aka social buffering. Here we studied social buffering in zebrafish, by exposing focal fish to an aversive stimulus (alarm substance - AS) either in the absence or presence of conspecific cues...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299288/food-safety-and-sustainable-nutrition-workshops-educational-experiences-for-primary-school-children-in-turin-italy
#9
Amaranta Traversa, Daniela Adriano, Alberto Bellio, Daniela Manila Bianchi, Silvia Gallina, Clara Ippolito, Angelo Romano, Paola Durelli, Andrea Pezzana, Lucia Decastelli
European control and prevention policies are focused to guarantee a high level of protection of consumers' health. Food-borne diseases as obesity, diabetes, food allergy, and food-borne outbreaks are increasing. To prevent food-borne diseases, it is fundamental to involve consumers, in particular children, in educational experiences aimed to learn the proper behaviours to be applied. In this context, we designed and performed 5 educational workshops about food safety, hidden allergens in food and nutrition aimed to involve children attending primary and summer school...
January 24, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28287609/assessing-the-risk-of-an-emerging-zoonosis-of-worldwide-concern-anisakiasis
#10
Miguel Bao, Graham J Pierce, Santiago Pascual, Miguel González-Muñoz, Simonetta Mattiucci, Ivona Mladineo, Paolo Cipriani, Ivana Bušelić, Norval J C Strachan
Anisakiasis is an emerging zoonosis caused by the fish parasitic nematode Anisakis. Spain appears to have the highest reported incidence in Europe and marinated anchovies are recognised as the main food vehicle. Using data on fishery landings, fish infection rates and consumption habits of the Spanish population from questionnaires, we developed a quantitative risk assessment (QRA) model for the anchovy value chain. Spaniards were estimated to consume on average 0.66 Anisakis per untreated (non-frozen) raw or marinated anchovy meal...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28262787/oscillations-and-accelerations-of-ice-crystal-growth-rates-in-microgravity-in-presence-of-antifreeze-glycoprotein-impurity-in-supercooled-water
#11
Yoshinori Furukawa, Ken Nagashima, Shun-Ichi Nakatsubo, Izumi Yoshizaki, Haruka Tamaru, Taro Shimaoka, Takehiko Sone, Etsuro Yokoyama, Salvador Zepeda, Takanori Terasawa, Harutoshi Asakawa, Ken-Ichiro Murata, Gen Sazaki
The free growth of ice crystals in supercooled bulk water containing an impurity of glycoprotein, a bio-macromolecule that functions as 'antifreeze' in living organisms in a subzero environment, was observed under microgravity conditions on the International Space Station. We observed the acceleration and oscillation of the normal growth rates as a result of the interfacial adsorption of these protein molecules, which is a newly discovered impurity effect for crystal growth. As the convection caused by gravity may mitigate or modify this effect, secure observations of this effect were first made possible by continuous measurements of normal growth rates under long-term microgravity condition realized only in the spacecraft...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28244005/sodium-selenite-prevents-paraquat-induced-neurotoxicity-in-zebrafish
#12
Talise E Müller, Mauro E Nunes, Charlene C Menezes, Aline T Marins, Jossiele Leitemperger, Ana Carolina Lopes Gressler, Fabiano B Carvalho, Catiuscia Molz de Freitas, Vanessa A Quadros, Roselei Fachinetto, Denis B Rosemberg, Vania L Loro
Considering the antioxidant properties of sodium selenite (Na2SeO3) and the involvement of oxidative stress events in paraquat-induced neurotoxicity, this study investigated the protective effect of dietary Na2SeO3 on biochemical and behavioral parameters of zebrafish exposed to paraquat (PQ). Fish were pretreated with a Na2SeO3 diet for 21 days and then PQ (20 mg/kg) was administered intraperitoneally with six injections for 16 days. In the novel tank test, the Na2SeO3 diet prevented the locomotor impairments, as well as the increase in the time spent in the top area of the tank, and the exacerbation of freezing episodes...
February 27, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28228353/isochoric-and-isobaric-freezing-of-fish-muscle
#13
Gabriel Năstase, Chenang Lyu, Gideon Ukpai, Alexandru Şerban, Boris Rubinsky
We have recently shown that, a living organism, which succumbs to freezing to -4 °C in an isobaric thermodynamic system (constant atmospheric pressure), can survive freezing to -4 °C in an isochoric thermodynamic system (constant volume). It is known that the mechanism of cell damage in an isobaric system is the freezing caused increase in extracellular osmolality, and, the consequent cell dehydration. An explanation for the observed survival during isochoric freezing is the thermodynamic modeling supported hypothesis that, in the isochoric frozen solution the extracellular osmolality is comparable to the cell intracellular osmolality...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28221955/synergistic-effect-of-freezing-and-irradiation-on-bonito-fish-sarda-sarda-bloch-1793
#14
Can Okan Altan, Hülya Turan
In this study, bonito fish ( Sarda sarda Bloch, 1793) were irradiated at 3 or 5 kGy, packaged, frozen, and stored at -20 ± 2°C for 12 months. During storage, the microbiological, physical, and chemical changes of the fish flesh were then assessed. Increasing the irradiation dose to 5 kGy provided greater inhibition of mesophilic and psychotropic aerobic bacteria (P < 0.05). Because fewer bacteria were detected in irradiated (3 and 5 kGy) as compared with unirradiated control fish suggests that freezing and irradiation yielded better results when combined...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28166981/sperm-quality-and-its-freezing-ability-throughout-the-spawning-season-in-prochilodus-lineatus-and-brycon-orbignyanus
#15
Isabela M Di Chiacchio, Izabella L G Almeida, Marcelo C Leal, Ana T M Viveiros
The aim of this study was to determine fresh and frozen sperm quality evaluated over two spawning seasons (2013-2014; 2014-2015) in Prochilodus lineatus and Brycon orbignyanus. The spawning seasons were divided into two sampling periods: November to December and January to February. Males were hand-stripped after carp pituitary treatment. Fresh sperm motility rate, velocities (curvilinear = VCL; straight-line = VSL; average path = VAP), and the beat cross frequency (BCF) were determined using a Computer-Assisted Sperm Analyzer (CASA)...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28098752/microbiological-load-of-edible-insects-found-in-belgium
#16
Rudy Caparros Megido, Sandrine Desmedt, Christophe Blecker, François Béra, Éric Haubruge, Taofic Alabi, Frédéric Francis
Edible insects are gaining more and more attention as a sustainable source of animal protein for food and feed in the future. In Belgium, some insect products can be found on the market, and consumers are sourcing fresh insects from fishing stores or towards traditional markets to find exotic insects that are illegal and not sanitarily controlled. From this perspective, this study aims to characterize the microbial load of edible insects found in Belgium (i.e., fresh mealworms and house crickets from European farms and smoked termites and caterpillars from a traditional Congolese market) and to evaluate the efficiency of different processing methods (blanching for all species and freeze-drying and sterilization for European species) in reducing microorganism counts...
January 13, 2017: Insects
https://www.readbyqxmd.com/read/28077930/before-the-freeze-otoliths-from-the-eocene-of-seymour-island-antarctica-reveal-dominance-of-gadiform-fishes-teleostei
#17
Werner Schwarzhans, Thomas Mörs, Andrea Engelbrecht, Marcelo Reguero, Jürgen Kriwet
The first record of fossil teleostean otoliths from Antarctica is reported. The fossils were obtained from late Early Eocene shell beds of the La Meseta Formation, Seymour Island that represent the last temperate marine climate phase in Antarctica prior to the onset of cooling and subsequent glaciation during the late Eocene. A total of 17 otolith-based teleost taxa are recognized, with 10 being identifiable to species level containing nine new species and one new genus: Argentina antarctica sp. nov., Diaphus? marambionis sp...
2017: Journal of Systematic Palaeontology
https://www.readbyqxmd.com/read/28077765/acute-embryonic-anoxia-exposure-favours-the-development-of-a-dominant-and-aggressive-phenotype-in-adult-zebrafish
#18
Catherine M Ivy, Cayleih E Robertson, Nicholas J Bernier
Eutrophication and climate change are increasing the incidence of severe hypoxia in fish nursery habitats, yet the programming effects of hypoxia on stress responsiveness in later life are poorly understood. In this study, to investigate whether early hypoxia alters the developmental trajectory of the stress response, zebrafish embryos were exposed to 4 h of anoxia at 36 h post-fertilization and reared to adults when the responses to secondary stressors were assessed. While embryonic anoxia did not affect basal cortisol levels or the cortisol response to hypoxia in later life, it had a marked effect on the responses to a social stressor...
January 11, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28063712/the-psychoactive-pollutant-fluoxetine-compromises-antipredator-behaviour-in-fish
#19
Jake M Martin, Minna Saaristo, Michael G Bertram, Phoebe J Lewis, Timothy L Coggan, Bradley O Clarke, Bob B M Wong
Pharmaceuticals are increasingly being detected in aquatic ecosystems worldwide. Particularly concerning are pharmaceutical pollutants that can adversely impact exposed wildlife, even at extremely low concentrations. One such contaminant is the widely prescribed antidepressant fluoxetine, which can disrupt neurotransmission and behavioural pathways in wildlife. Despite this, relatively limited research has addressed the behavioural impacts of fluoxetine at ecologically realistic exposure concentrations. Here, we show that 28-day fluoxetine exposure at two ecologically relevant dosages-one representing low surface water concentrations and another representing high effluent flow concentrations-alters antipredator behaviour in Eastern mosquitofish (Gambusia holbrooki)...
March 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28012381/automated-milk-fat-extraction-for-the-analyses-of-persistent-organic-pollutants
#20
Jeffrey C Archer, Roy G Jenkins
We have utilized an automated acid hydrolysis technology, followed by an abbreviated Soxhlet extraction technique to obtain fat from whole milk for the determination of persistent organic pollutants, namely polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls. The process simply involves (1) pouring the liquid milk into the hydrolysis beaker with reagents and standards, (2) drying the obtained fat on a filter paper and (3) obtaining pure fat via the modified Soxhlet extraction using 100mL of hexane per sample...
January 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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