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https://www.readbyqxmd.com/read/29779063/development-of-sperm-vitrification-protocols-for-two-endangered-salmonid-species-the-adriatic-grayling-thymallus-thymallus-and-the-marble-trout-salmo-marmoratus
#1
Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29750975/nicotine-increases-fear-responses-and-brain-acetylcholinesterase-activity-in-a-context-dependent-manner-in-zebrafish
#2
Paola R Ziani, Talise E Müller, Flavia V Stefanello, Barbara D Fontana, Tâmie Duarte, Julia Canzian, Denis B Rosemberg
Nicotine is an alkaloid with positive effects on learning and memory processes. Exposure to conspecific alarm substance (CAS) elicits fear responses in zebrafish, but the effects of nicotine on aversive behaviors and associative learning in this species remain unclear. Here, we evaluated whether nicotine enhances contextual fear responses in zebrafish and investigated a putative involvement of brain acetylcholinesterase (AChE) in associative learning. Fish were exposed to 1 mg/L nicotine for 3 min and then kept in non-chlorinated water for 20 min...
May 8, 2018: Pharmacology, Biochemistry, and Behavior
https://www.readbyqxmd.com/read/29739320/mobilization-of-retrotransposons-as-a-cause-of-chromosomal-diversification-and-rapid-speciation-the-case-for-the-antarctic-teleost-genus-trematomus
#3
J Auvinet, P Graça, L Belkadi, L Petit, E Bonnivard, A Dettaï, W H Detrich, C Ozouf-Costaz, D Higuet
BACKGROUND: The importance of transposable elements (TEs) in the genomic remodeling and chromosomal rearrangements that accompany lineage diversification in vertebrates remains the subject of debate. The major impediment to understanding the roles of TEs in genome evolution is the lack of comparative and integrative analyses on complete taxonomic groups. To help overcome this problem, we have focused on the Antarctic teleost genus Trematomus (Notothenioidei: Nototheniidae), as they experienced rapid speciation accompanied by dramatic chromosomal diversity...
May 9, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29734427/canonical-correlation-analysis-to-identify-the-semen-characteristics-used-to-forecast-the-freeze-survival-of-curimba-prochilodus-lineatus-spermatozoa
#4
A F S Carvalho, L D S Murgas, M R Ferreira-Machad, E S Andrade, V O Felizardo, I B Allaman, F G de Paula
  OBJECTIVE: To identify which sperm characteristics were able to predict more accurately the quality of curimba (Prochilodus lineatus) semen upon freezing using canonical correlation analysis. METHODS: Eleven fish breeders with initial mean weight of 705.21 ± 111 g were used. For cryopreservation, 200 µL of semen were taken from each animal and diluted in the cryoprotectant solution (10% dimethyl sulfoxide and 5% Beltsville Thawing Solution Minitub) in a 1:4 ratio and placed into 0...
July 2017: Cryo Letters
https://www.readbyqxmd.com/read/29734415/effective-condition-for-whole-testis-cryopreservation-of-endangered-miho-spine-loach-cobitis-choii-through-the-optimization-of-mud-loach-misgurnus-mizolepis-whole-testis-cryopreservation-condition
#5
J J Kim, Y K Nam, I C Bang, S P Gong
  BACKGROUND: Miho spine loach (Cobitis choii) is an endangered Korean endemic fish. Whole testis cryopreservation is a good way for species preservation, but needs to the sacrifice of a large number of fish to optimize the freezing condition. Considering this limitation, a surrogate fish species was used for the protocol development. OBJECTIVE: This study was to establish the effective condition for Miho spine loach whole testis cryopreservation by optimizing the conditions for whole testis cryopreservation in an allied species, mud loach (Misgurnus mizolepis)...
January 2018: Cryo Letters
https://www.readbyqxmd.com/read/29678228/analyzing-complement-activity-in-the-serum-and-body-homogenates-of-different-fish-species-using-rabbit-and-sheep-red-blood-cells
#6
Sagar Nayak, Isabel Portugal, Dina Zilberg
Alternative complement activity was determined in whole body homogenates (WBHs) and serum samples of different fish species, by measuring the amount of sample that induces 50% hemolysis of red blood cells using the ACH50 assay (Alternative Complement pathway Hemolytic activity). Values of ACH50 obtained for serum samples were about two-fold higher when using rabbit red blood cells (RRBC), as compared to sheep red blood cells (SRBC). The increase in ACH50 when using RRBCs for WBH samples was 28, 7 and 4 folds for guppy, molly and zebrafish, respectively...
May 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29651197/a-strategy-for-sperm-cryopreservation-of-atlantic-salmon-salmo-salar-for-remote-commercial-scale-high-throughput-processing
#7
Huiping Yang, E Hu, John T Buchanan, Terrence R Tiersch
Sperm cryopreservation is an essential tool for long-term storage of genetic resources for aquaculture fishes. The goal of this study was to develop an efficient and streamlined protocol for high-throughput processing for sperm cryopreservation in Atlantic salmon, Salmo salar . The objectives were to evaluate: 1) osmolality of blood serum for determining extender osmolality; 2) effects of extenders for fresh sperm dilution and refrigerated storage; 3) effects of methanol and dimethyl sulfoxide (DMSO) on fresh sperm motility, and 4) motility and fertility after thawing...
February 2018: Journal of the World Aquaculture Society
https://www.readbyqxmd.com/read/29589277/effects-of-microencapsulated-saccharomyces-cerevisiae-on-growth-hematological-indices-blood-chemical-and-immune-parameters-and-intestinal-morphology-in-striped-catfish-pangasianodon-hypophthalmus
#8
Surintorn Boonanuntanasarn, Khanittha Ditthab, Araya Jangprai, Chatsirin Nakharuthai
This study investigated the effects of dietary probiotic Saccharomyces cerevisiae in the striped catfish, Pangasianodon hypophthalmus, which is an important aquaculture species. Freeze-dried microencapsulated probiotic S. cerevisiae with guar gum was performed and used for fish feed supplementation. Striped catfish were fed for 120 days with one of three experimental diets: basal diet (control), basal diet supplemented with 106 -CFU S. cerevisiae g-1 diet (S. cerevisiae 106 ), and basal diet supplemented with 108 -CFU S...
March 27, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29577677/phase-determination-using-chromosomal-microarray-and-fluorescence-in-situ-hybridization-in-a-patient-with-early-onset-parkinson-disease-and-two-deletions-in-prkn
#9
Eli S Williams, Matthew J Barrett, Radhika Dhamija, Lisa Toran, Chelsea Chambers, Mani S Mahadevan, Wendy L Golden
BACKGROUND: Mutations in the parkin gene (PRKN) are the most commonly identified genetic factors in early onset Parkinson disease (EOPD), with biallelic mutations, resulting in a clinical phenotype. However, normal variation is also common in PRKN, particularly in the form of copy number variation (CNV), challenging interpretation of genetic testing results. Here we report a case of a 29-year-old male with EOPD and two deletions in PRKN detected by chromosomal microarray (CMA). METHODS: The proband was clinically examined by a neurologist for postural instability with frequent falls, bradykinesia, gait freezing with festination, and hypophonia...
March 25, 2018: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29567443/simultaneous-determination-of-21-trace-perfluoroalkyl-substances-in-fish-by-isotope-dilution-ultrahigh-performance-liquid-chromatography-tandem-mass-spectrometry
#10
Yan Gao, Xiaomin Li, Xiuqin Li, Qinghe Zhang, Hongmei Li
Perfluoroalkyl substances (PFASs) have been identified as emerging environmental contaminants. In this study, an efficient and accurate ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous determination of 21 PFASs in fish. Acetonitrile was used for sample extraction. Solid phase extraction (SPE) by WAX cartridges and then freezing at -30 °C were adopted as cleanup strategies. Strict measurements were performed to control background contamination during the whole procedure...
March 7, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29529585/three-dimensional-polylactic-acid-graphene-oxide-chitosan-sponge-bionic-filter-highly-efficient-adsorption-of-crystal-violet-dye
#11
G Zhou, K P Wang, H W Liu, L Wang, X F Xiao, D D Dou, Y B Fan
Owing to low bearing capacity and efficiency, traditional filters or adsorbents for removal of contaminants like crystal violet (CV) dye required frequent replacement. Besides, the combination of three-dimensional (3D) printing and bionics could break the constraints of traditional configuration. In this study, a novel depth-type hybrid polylactic acid (PLA)@graphene oxide (GO)/chitosan (CS) sponge filter with bionic fish-mouth structure was prepared and fabricated, assisted by 3D printing and double freeze-drying technology, according to the theories of vertical cross-step filtration and swirling flow...
July 1, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29516860/methodological-issues-affecting-the-study-of-fish-parasites-iii-effect-of-fish-preservation-method
#12
Yuriy Kvach, Markéta Ondračková, Michal Janáč, Pavel Jurajda
The aim of this study was to evaluate the influence of preservation method on the results of parasite community studies. Two host species, European perch Perca fluviatilis and European bitterling Rhodeus amarus, were examined for parasites after having been subjected to 4 different storage treatments: freezing, preservation in 4% formaldehyde or 70% ethanol and transportation of live (fresh) fish as a control. Preservation prior to dissection resulted in a loss of information, leading to incomplete quantitative data (all preservation treatments), qualitative data (ethanol and formaldehyde preservation) and a lowered ability to determine parasites to species level based on morphology compared to dissecting fresh fish...
March 5, 2018: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29510906/reconstruction-of-the-repetitive-antifreeze-glycoprotein-genomic-loci-in-the-cold-water-gadids-boreogadus-saida-and-microgadus-tomcod
#13
Xuan Zhuang, Katherine R Murphy, Laura Ghigliotti, Eva Pisano, C-H Christina Cheng
Antifreeze glycoproteins (AFGPs) are a novel evolutionary innovation in members of the northern cod fish family (Gadidae), crucial in preventing death from inoculative freezing by environmental ice in their frigid Arctic and sub-Arctic habitats. However, the genomic origin and molecular mechanism of evolution of this novel life-saving adaptive genetic trait remained to be definitively determined. To this end, we constructed large insert genomic DNA BAC (bacterial artificial chromosome) libraries for two AFGP-bearing gadids, the high-Arctic polar cod Boreogadus saida and the cold-temperate Atlantic tomcod Microgadus tomcod, to isolate and sequence their AFGP genomic regions for fine resolution evolutionary analyses...
March 3, 2018: Marine Genomics
https://www.readbyqxmd.com/read/29478227/heat-adaptation-improved-cell-viability-of-probiotic-enterococcus-faecium-hl7-upon-various-environmental-stresses
#14
YuJin Shin, Chang-Ho Kang, Woori Kim, Jae-Seong So
The production of viable functional probiotics presupposes stability of strain features in the final product. In previous studies, Enterococcus faecium HL7 was found to have relatively higher cell viability after freeze-drying and the long-lasting resistance to heat (60 °C) as well as higher antimicrobial activities against some of fish and human pathogens among isolated strains. For heat adaptation, E. faecium HL7 cells were exposed to 52 °C for 15 min. After adaption, slight decreases of unsaturated membrane fatty acid ratios were confirmed through fatty acid analysis...
February 24, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29465759/development-of-a-method-to-preliminarily-embed-tissue-samples-using-low-melting-temperature-fish-gelatin-before-sectioning-a-technical-note
#15
Kaori Ushida, Naoya Asai, Kozo Uchiyama, Atsushi Enomoto, Masahide Takahashi
Embedding of tissue samples that maintains a desired orientation is critical for preparing sections suitable for diagnosis and study objectives. Methods to prepare tissue sections include: (i) paraffin embedding or snap-freezing followed by microtome or cryostat sectioning; and (ii) agarose embedding followed by cutting on a vibrating microslicer. Although these methods are useful for routine laboratory work, preparation of small and fragile tissues such as mouse organs, small human biopsy samples, and cultured floating spheres is difficult and requires special skills...
April 2018: Pathology International
https://www.readbyqxmd.com/read/29409977/extending-the-analysis-of-zebrafish-behavioral-endophenotypes-for-modeling-psychiatric-disorders-fear-conditioning-to-conspecific-alarm-response
#16
Caio Maximino, Daniele L Meinerz, Barbara D Fontana, Nathana J Mezzomo, Flavia V Stefanello, Alessandro de S Prestes, Cibele B Batista, Maribel A Rubin, Nilda V Barbosa, João Batista T Rocha, Monica G Lima, Denis B Rosemberg
Anxiety, trauma- and stressor-related disorders are severe psychiatric conditions that affect human population worldwide. Given their genetic tractability, evolutionarily conserved neurotransmitter systems, and extensive behavioral repertoire, zebrafish have become an emergent model organism in translational neuroscience. Here, we investigate whether a single exposure to conspecific alarm substance (CAS) produces fear conditioning in zebrafish using a conditioned place aversion (CPA) paradigm, as well as the persistence of aversive responses at different time intervals...
April 2018: Behavioural Processes
https://www.readbyqxmd.com/read/29407809/development-of-a-lipovitellin-based-sandwich-elisa-for-determination-of-vitellogenin-in-the-marine-medaka-oryzias-melastigma
#17
Xianliang Yi, Changlin Li, Xi Zhong, Yufeng Gong
A lipovitellin (Lv) based sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in marine medaka (Oryzias melastigma). Lv and Vtg were purified from the unfertilized eggs and the whole body homogenates (WBH) of estradiol (E2 )-exposed fish. The purified Lv sample appeared as three clear bands (118, 112 and 100 kDa) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and was identified as an Lvs mixture from VtgAa and VtgAb by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis...
April 2018: Chemosphere
https://www.readbyqxmd.com/read/29381426/real-time-cameraless-measurement-system-based-on-bioelectrical-ventilatory-signals-to-evaluate-fear-and-anxiety
#18
Zu Soh, Motoki Matsuno, Masayuki Yoshida, Toshio Tsuji
Fear and anxiety in fish are generally evaluated by video-based behavioral analysis. However, it is difficult to distinguish the psychological state of fish exclusively through video analysis, particularly whether the fish are freezing, which represents typical fear behavior, or merely resting. We propose a system that can measure bioelectrical signals called ventilatory signals and simultaneously analyze swimming behavior in real time. Experimental results comparing the behavioral analysis of the proposed system and the camera system showed a low error level with an average absolute position error of 9...
April 2018: Zebrafish
https://www.readbyqxmd.com/read/29369747/survival-of-bacterial-and-parasitic-pathogens-from-zebrafish-danio-rerio-after-cryopreservation-and-thawing
#19
Lauren J Norris, Virginia Watral, Michael L Kent
Cryopreservation is a common method used to preserve the sperm of various animal species, and it is widely used with zebrafish (Danio rerio). As with other animals, there is a possibility of paternal pathogen transmission through sperm. We evaluated the ability of five common and important pathogens of zebrafish to survive cryopreservation as used with zebrafish sperm and freezing without cryopreservant. We evaluated Mycobacterium chelonae, Mycobacterium marinum, and Edwardsiella ictaluri, each originally isolated from zebrafish, eggs of Pseuodocapillaria tomentosa, and spores of Pseudoloma neurophilia...
April 2018: Zebrafish
https://www.readbyqxmd.com/read/29369687/a-limited-survey-of-heavy-metal-concentrations-in-fresh-and-frozen-cuttlefish-ink-and-mantle-used-as-food
#20
Daniele Conficoni, Leonardo Alberghini, Elisa Bissacco, Barbara Contiero, Valerio Giaccone
Cuttlefish ink is consumed as a delicacy worldwide. The current study is the first assessment of heavy metal concentrations in cuttlefish ink versus mantle under different storage methods. A total of 212 samples (64 of fresh mantle, 42 of frozen mantle, 64 of fresh ink, and 42 of frozen ink) were analyzed for the detection of the following heavy metals: arsenic (As), chromium (Cr), iron (Fe), lead (Pb), mercury (Hg), and cadmium (Cd). The median As concentrations were 12.9 mg/kg for fresh mantle, 8.63 mg/kg for frozen mantle, 10...
February 2018: Journal of Food Protection
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