Diogo Tiago da Silva, Maria Luana Alves, Júlio Cesar Pereira Spada, João Augusto Franco Leonel, Geovanna Vioti, Julia Cristina Benassi, Valéria Maria Lara Carregaro, Maria Fernanda Alves-Martin, Wilma Aparecida Starke-Buzetti, Trícia Maria Ferreira de Sousa Oliveira
One hundred and sixty-six cats from two animal shelters were subjected to enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), conventional polymerase chain reaction (cPCR), quantitative PCR (qPCR) and parasitological tests (PA) for the diagnosis of Leishmania spp. Among them, 15% (25/166), 53.6% (89/166), 3.6% (06/166) and 1.8% (03/166) were positive by ELISA, IFAT, both PCRs and PA, respectively. The sequencing of ITS-1 PCR amplicons revealed a 100% match with Leishmania infantum...
2023: Brazilian Journal of Veterinary Parasitology