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Carolina Hernández, Zulma Cucunubá, Carolina Flórez, Mario Olivera, Carlos Valencia, Pilar Zambrano, Cielo León, Juan David Ramírez
BACKGROUND: The diagnosis of Chagas disease is complex due to the dynamics of parasitemia in the clinical phases of the disease. The molecular tests have been considered promissory because they detect the parasite in all clinical phases. Trypanosoma cruzi presents significant genetic variability and is classified into six Discrete Typing Units TcI-TcVI (DTUs) with the emergence of foreseen genotypes within TcI as TcIDom and TcI Sylvatic. The objective of this study was to determine the operating characteristics of molecular tests (conventional and Real Time PCR) for the detection of T...
September 2016: PLoS Neglected Tropical Diseases
Tegene Negesse, Chander Datt, S S Kundu
A feeding trial with 18 Murrah buffalo heifers (238 ± 63 kg; 1.7 ± 0.4 years old) was conducted for 57 days, and feed intake and body weight were recorded followed by 7 days of urine and feces collection. Heifers were grouped into low (RFI-I) and high (RFI-II) feed efficiency groups by regressing DMI (g head(-1) day(-1)) on BWG (g head(-1) day(-1)) and mid-test MBW (kg). RFI-II heifers had slightly higher DMI (g head(-1) day(-1) and expressed per unit of BW, %BW, MBW, mid-test BW, and mid-test MBW) and all associated nutrient intakes, BWG, feed efficiency traits (FCR, CPCR), digestibility of nutrients, and retentions of crude protein and fat over RFI-I heifers...
September 12, 2016: Tropical Animal Health and Production
Candice Sant, Roger d'Abadie, Indira Pargass, Asoke K Basu, Zinora Asgarali, Roxanne A Charles, Karla C Georges
Equine piroplasmosis caused by Theileria equi and Babesia caballi is endemic in Trinidad and Tobago. Transmission occurs by ticks of the family Ixodidae. T. equi can also be transmitted transplacentally; however transplacental transmission of B. caballi is unknown. This study aims to investigate transplacental transmission of equine piroplasmosis from thoroughbred mares naturally infected via the tick vector. Whole blood and serum samples were collected from 117 mares in the fifth month of pregnancy. Blood samples were also collected from each of their foals (89 in total) within the first 36h of birth...
August 15, 2016: Veterinary Parasitology
R Ryan McNally, Carol A Ishimaru, Dean K Malvick
Goss's leaf blight and wilt of maize (corn) is a significant and reemerging disease caused by the bacterium Clavibacter michiganensis subsp. nebraskensis. Despite its importance, molecular tools for diagnosing and studying this disease remain limited. We report the identification of CMN_01184 as a novel gene target and its use in conventional PCR (cPCR) and SYBR green-based quantitative PCR (qPCR) assays for specific detection and quantification of C. michiganensis subsp. nebraskensis. The cPCR and qPCR assays based on primers targeting CMN_01184 specifically amplified only C...
October 11, 2016: Phytopathology
Shahram Bolandparvaz, Hamid Mohajer, Mansoor Masjedi, Ehsan Mohammadhoseini, Leila Shayan
OBJECTIVES: To determine the correlation between the success rates of the cardiopulmonary cerebral resuscitation (CPCR) and the team's leader education and skill level in Shiraz, southern Iran. METHOD: This cross-sectional study was conducted during a 6-month period from October 2007 to March 2008 in Nemazee hospital of Shiraz. We included all the patients who underwent CPCR due to cardiopulmonary arrest in emergency room of Nemazee hospital during the study period...
October 2015: Bulletin of Emergency and Trauma
Jinfeng Wang, Wei Ma, Yuping Xie, Peilin Hui, Lijun Zhao, Xiaoquan Wei, Liya Zhou
OBJECTIVE: To evaluate the curative effect of continuous positive airway pressure(CPAP) combined with modified oral appliance (MOA) in the treatment of severe OSAHS. METHOD: The research chose 120 male patients with severe OSAHS. They were treated with CPAP and MOA (30 cases), CPAP (30 cases), and MOA (30 cases) respectively. At the same time, the rest 30 cases without any treatment were in control group (matching factors: age, gender, IBM). After 3 months treatment, the characteristics of PSG and respiratory machine wear condition were observated in four groups...
December 2015: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Shinichiro Mori, Takuji Furukawa
To shorten treatment time in pencil beam scanning irradiation, we developed rapid phase-controlled rescanning (rPCR), which irradiates two or more isoenergy layers in a single gating window. Here, we evaluated carbon-ion beam dose distribution with rapid and conventional PCR (cPCR). 4 dimensional computed tomography (4DCT) imaging was performed on 12 subjects with lung or liver tumors. To compensate for intrafractional range variation, the field-specific target volume (FTV) was calculated using 4DCT within the gating window (T20-T80)...
May 21, 2016: Physics in Medicine and Biology
Jung Lo, Po-Chiung Fang, Chun-Chih Chien, Chang-Chun Hsiao, Shin-Ling Tseng, Yu-Hsuan Lai, Ming-Tse Kuo
PURPOSE: To develop a PCR gel analysis method for assessing the bacterial bioburden in orthokeratology contact lens (OK) case fluid determined by culture. METHODS: A prospective study with the participation of 41 OK wearers (20 girls, 21 boys) was performed. The mean OK-wearing experience was 3.5±1.9 years. PCR was used to assess the bacterial bioburden (colony-forming units per milliliter) of OK after removal and soaking in the storage case for 6 h. The signal intensity of the PCR bands was analyzed after grayscale image transformation...
2016: Molecular Vision
N B Vanasco, P Jacob, N Landolt, Y Chiani, M F Schmeling, C Cudos, H Tarabla, J Lottersberger
Enzyme-linked immunosorbent assay (ELISA) tests and polymerase chain reaction (PCR) may play a key role for early detection and treatment of human leptospirosis in developing countries. The aims of this study were to develop and validate an IgM ELISA under field conditions and to compare the diagnostic accuracy among IgG, IgM ELISAs, conventional PCR (cPCR), and real-time PCR (rtPCR) for early detection of human leptospirosis. Overall accuracy of IgM ELISA was sensitivity of 87.9%, specificity of 97.0%, and area under the curve of 0...
April 2016: Diagnostic Microbiology and Infectious Disease
P B Shridhar, L W Noll, X Shi, B An, N Cernicchiaro, D G Renter, T G Nagaraja, J Bai
Shiga toxin-producing Escherichia coli (STEC) serogroups O26, O45, O103, O111, O121, and O145, called non-O157 STEC, are important foodborne pathogens. Cattle, a major reservoir, harbor the organisms in the hindgut and shed them in the feces. Although limited data exist on fecal shedding, concentrations of non-O157 STEC in feces have not been reported. The objectives of our study were (i) to develop and validate two multiplex quantitative PCR (mqPCR) assays, targeting O-antigen genes of O26, O103, and O111 (mqPCR-1) and O45, O121, and O145 (mqPCR-2); (ii) to utilize the two assays, together with a previously developed four-plex qPCR assay (mqPCR-3) targeting the O157 antigen and three virulence genes (stx1, stx2, and eae), to quantify seven serogroups and three virulence genes in cattle feces; and (iii) to compare the three mqPCR assays to a 10-plex conventional PCR (cPCR) targeting seven serogroups and three virulence genes and culture methods to detect seven E...
January 2016: Journal of Food Protection
Mariana Previato, Fábio Batista Frederico, Fernando Henrique Antunes Murata, Rubens Camargo Siqueira, Amanda Pires Barbosa, Aparecida Perpétuo Silveira-Carvalho, Cristina da Silva Meira, Vera Lúcia Pereira-Chioccola, Ricardo Gava, Plínio Pereira Martins Neto, Luiz Carlos de Mattos, Cinara Cássia Brandão de Mattos
BACKGROUND: Toxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging. The aim of this study was to evaluate the use of serology and molecular methods to monitor acute OT in immunocompetent patients during treatment. METHODS: Five immunocompetent patients were clinically diagnosed with acute OT...
December 7, 2015: BMC Research Notes
Marcus Beissner, Richard Odame Phillips, Florian Battke, Malkin Bauer, Kossi Badziklou, Fred Stephen Sarfo, Issaka Maman, Agata Rhomberg, Ebekalisai Piten, Michael Frimpong, Kristina Lydia Huber, Dominik Symank, Moritz Jansson, Franz Xaver Wiedemann, Abiba Banla Kere, Karl-Heinz Herbinger, Thomas Löscher, Gisela Bretzel
BACKGROUND: As the major burden of Buruli ulcer disease (BUD) occurs in remote rural areas, development of point-of-care (POC) tests is considered a research priority to bring diagnostic services closer to the patients. Loop-mediated isothermal amplification (LAMP), a simple, robust and cost-effective technology, has been selected as a promising POC test candidate. Three BUD-specific LAMP assays are available to date, but various technical challenges still hamper decentralized application...
November 2015: PLoS Neglected Tropical Diseases
Lance W Noll, Pragathi B Shridhar, Xiaorong Shi, Baoyan An, Natalia Cernicchiaro, David G Renter, Tiruvoor G Nagaraja, Jianfa Bai
Several real-time polymerase chain reaction (PCR) assays have been developed to detect and quantify Shiga toxin-producing Escherichia coli (STEC) O157:H7, but none have targeted the O-antigen specific gene (rfbEO157) in combination with the three major virulence genes, stx1, stx2, and eae. Our objectives were to develop and validate a four-plex, quantitative PCR (mqPCR) assay targeting rfbE(O157), stx1, stx2, and eae for the detection and quantification of STEC O157 in cattle feces, and compare the applicability of the assay to detect STEC O157 to a culture method and conventional PCR (cPCR) targeting the same four genes...
September 2015: Foodborne Pathogens and Disease
Hamed Hasani, Mojtaba Bahrami, Abdorrasoul Malekpour, Mohammadreza Dehghani, Elaheh Allahyary, Mitra Amini, Mehdi Abdorahimi, Sara khani, Mohammad Kalantari Meibodi, Javad Kojuri
To determine the efficacy of different methods of cardiopulmonary resuscitation (CPCR) training in 3 different groups of the society. In a prospective and observational study of 2000 individuals in 3 different groups including G1, G2, and G3 4 different protocols of CPCR training were applied and their efficacy was compared between the groups. Also, 12 months after the study course, 460 participants from 3 groups were asked to take apart in a theoretical and practical examination to evaluate the long-term efficacy of the 4 protocols...
May 2015: Medicine (Baltimore)
Fabiana Martins de Paula, Fernanda de Mello Malta, Priscilla Duarte Marques, Renata Barnabé Sitta, João Renato Rebello Pinho, Ronaldo César Borges Gryschek, Pedro Paulo Chieffi
This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR...
April 2015: Memórias do Instituto Oswaldo Cruz
Danielle Gava, Carine K Souza, Rejane Schaefer, Amy L Vincent, Maurício E Cantão, Arlei Coldebella, Janice R Ciacci-Zanella
Porcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqMan-based real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 10(1) DNA copies/μL. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus...
July 2015: Journal of Virological Methods
Fabiana Martins de Paula, Fernanda de Mello Malta, Priscilla Duarte Marques, Renata Barnabé Sitta, João Renato Rebello Pinho, Ronaldo César Borges Gryschek, Pedro Paulo Chieffi
This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR...
March 6, 2015: Memórias do Instituto Oswaldo Cruz
Heather L Farrington, Christine E Edwards, Xin Guan, Matthew R Carr, Kelly Baerwaldt, Richard F Lance
Invasive Asian bighead and silver carp (Hypophthalmichthys nobilis and H. molitrix) pose a substantial threat to North American aquatic ecosystems. Recently, environmental DNA (eDNA), genetic material shed by organisms into their environment that can be detected by non-invasive sampling strategies and genetic assays, has gained recognition as a tool for tracking the invasion front of these species toward the Great Lakes. The goal of this study was to develop new species-specific conventional PCR (cPCR) and quantitative (qPCR) markers for detection of these species in North American surface waters...
2015: PloS One
Dora Ordóñez, Natalia Aragón, Luz Stella García, Paola Collazos, Luis Eduardo Bravo
OBJECTIVE: To describe the time trends of the incidence and mortality rates of oral cancer (OC) in Cali, Colombia between 1962-2007. MATERIALS AND METHODS: Age-standardized (Segi's world population) incidence (ASIR) and mortality (ASMR) rates for oral cancer were estimated using data from the Population-based Cancer Registry of Cali, Colombia and from the database of the Municipal Secretary of Public Health (MSPH) respectively. Annual percentage change (APC) was used to measure the changes in rates over time...
September 2014: Salud Pública de México
D R Bogema, A T Deutscher, S Fell, D Collins, G J Eamens, C Jenkins
Theileria orientalis is an emerging pathogen of cattle in Asia, Australia, and New Zealand. This organism is a vector-borne hemoprotozoan that causes clinical disease characterized by anemia, abortion, and death, as well as persistent subclinical infections. Molecular methods of diagnosis are preferred due to their sensitivity and utility in differentiating between pathogenic and apathogenic genotypes. Conventional PCR (cPCR) assays for T. orientalis detection and typing are laborious and do not provide an estimate of parasite load...
March 2015: Journal of Clinical Microbiology
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