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https://www.readbyqxmd.com/read/29157370/bayesian-estimation-of-true-prevalence-sensitivity-and-specificity-of-three-diagnostic-tests-for-detection-of-escherichia-coli-o157-in-cattle-feces
#1
Pius S Ekong, Michael W Sanderson, Nora M Bello, Lance W Noll, Natalia Cernicchiaro, David G Renter, Jianfa Bai, T G Nagaraja
Cattle are a reservoir for Escherichia coli O157 and they shed the pathogen in their feces. Fecal contaminants on the hides can be transferred onto carcasses during processing at slaughter plants, thereby serving as a source of foodborne infection in humans. The detection of E. coli O157 in cattle feces is based on culture, immunological, and molecular methods We evaluated the diagnostic sensitivity and specificity of one culture- and two PCR-based tests for the detection of E. coli O157 in cattle feces, and its true prevalence using a Bayesian implementation of latent class models...
December 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29151422/-successful-perform-of-prolonged-cardiopulmonary-and-cerebral-resuscitation-on-repeated-cardiac-arrest-one-case-report
#2
Guanghui He, Yanping Liang, Jianjun Jing, Xiaofeng Li, Xin Jin, Yicheng Yang, Shibin Lyu, Jiaojiao Lu
Cardiac arrest (CA) is the most serious clinical emergency situation and cardiopulmonary-cerebral resuscitation (CPCR) performed on site with high quality is the optional therapy for its management. It has been reported that prolonging the resuscitation time after 30-minute failed conventional cardiopulmonary resuscitation (CPR) could improve the in-hospital survival rate of CA patients, and how to improve the out-hospital survival rate and survival quality of these patients is a research hot focus at present...
November 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29127991/arthropod-borne-agents-in-wild-orinoco-geese-neochen-jubata-in-brazil
#3
Karin Werther, Mayara de Cássia Luzzi, Luiz Ricardo Gonçalves, Juliana Paula de Oliveira, José Roberto Ferreira Alves Junior, Rosangela Zacarias Machado, Marcos Rogério André
Although Orinoco goose (Neochen jubata) is an anatid species widely distributed in South America, scarce are the reports on the occurrence of arthropod-borne pathogens in this avian species. The present work aimed to verify, by serological and molecular methods, the occurrence of haemosporida piroplasmids and Anaplasmataceae agents in wild Orinoco geese captured in Brazil. Between 2010 and 2014, 62 blood samples were collected from free-living geese captured in the Araguaia River, Goiás State, Brazil. Six geese (10%) were seropositive for Anaplasma phagocytophilum, showing titers ranging from 40 and 80...
December 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/29111373/rickettsia-spp-among-wild-mammals-and-their-respective-ectoparasites-in-pantanal-wetland-brazil
#4
Keyla Carstens Marques de Sousa, Heitor Miraglia Herrera, Fabiana Lopes Rocha, Francisco Borges Costa, Thiago Fernandes Martins, Marcelo Bahia Labruna, Rosangela Zacarias Machado, Marcos Rogério André
The genus Rickettsia comprises obligatory intracellular bacteria, well known to cause zoonotic diseases around the world. The present work aimed to investigate the occurrence of Rickettsia spp. in wild animals, domestic dogs and their respective ectoparasites in southern Pantanal region, central-western Brazil, by molecular and serological techniques. Between August 2013 and March 2015, serum, whole blood and/or spleen samples were collected from 31 coatis, 78 crab-eating foxes, seven ocelots, 42 dogs, 110 wild rodents, and 30 marsupials...
October 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29017647/-early-evaluation-of-patients-with-amplitude-integrated-electroencephalogram-on-brain-function-prognosis-after-cardiopulmonary-cerebral-resuscitation
#5
Xin Dong, Huanzhang Shao, Yanan Yang, Lijie Qin, Zhisong Guo, Huifeng Zhang, Xueyan Zhang, Bingyu Qin
OBJECTIVE: To explore the characteristic of early evaluation of patients with amplitude-integrated electroencephalogram (aEEG) on brain function prognosis after cardiopulmonary cerebral resuscitation (CPCR). METHODS: A retrospective analysis of the clinical data of patients with adult CPCR in intensive care unit (ICU) of Henan Provincial People's Hospital from March 2016 to March 2017 was performed. The length of stay, recovery time, acute physiology and chronic health evaluation II (APACHE II) score, aEEG and Glasgow coma scale (GCS) within 72 hours were recorded...
October 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29016341/prevalence-of-clinical-and-subclinical-plasmodium-falciparum-and-plasmodium-vivax-malaria-in-two-remote-rural-communities-on-the-china-myanmar-border
#6
Fang Huang, Shannon Takala-Harrison, Hui Liu, Jian-Wei Xu, Heng-Lin Yang, Matthew Adams, Biraj Shrestha, Gillian Mbambo, Demian Rybock, Shui-Sen Zhou, Zhi-Gui Xia, Xiao-Nong Zhou, Christopher V Plowe, Myaing M Nyunt
Malaria infections may be symptomatic, leading to treatment, or "asymptomatic," typically detected through active surveillance, and not leading to treatment. Malaria elimination may require purging both types of infection. Using detection methods with different sensitivities, we conducted a cross-sectional study in two rural communities located along the border between China's Yunnan Province and Myanmar's Shan and Kachin States, to estimate the prevalence of asymptomatic and symptomatic malaria. In Mong Pawk, all infections detected were asymptomatic, and the prevalence of Plasmodium falciparum was 0...
September 5, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28977247/genetic-diversity-of-piroplasmids-species-in-equids-from-island-of-s%C3%A3-o-lu%C3%A3-s-northeastern-brazil
#7
Maria do Socorro Costa de Oliveira Braga, Francisca Neide Costa, Débora Regina Maia Gomes, Daniele Rosa Xavier, Marcos Rogério André, Luiz Ricardo Gonçalves, Carla Roberta Freschi, Rosangela Zacarias Machado
Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules)...
July 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28968510/molecular-diagnosis-of-symptomatic-toxoplasmosis-a-9-year-retrospective-and-prospective-study-in-a-referral-laboratory-in-s%C3%A3-o-paulo-brazil
#8
Lilian Muniz Camilo, Vera Lucia Pereira-Chioccola, Ricardo Gava, Cristina da Silva Meira-Strejevitch, Jose Ernesto Vidal, Cinara Cássia Brandão de Mattos, Fábio Batista Frederico, Luiz Carlos De Mattos, Lígia Cosentino Junqueira Franco Spegiorin
Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis...
September 29, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28906180/amplification-of-mycoplasma-haemofelis-dna-by-a-pcr-for-point-of-care-use
#9
Jennifer Hawley, Tal Yaaran, Sarah Maurice, Michael R Lappin
We compared a qualitative in-clinic (IC)-PCR for the detection of Mycoplasma haemofelis DNA with the results of a commercial qualitative laboratory-based, conventional (c)PCR. In order to determine the specificity of both tests, Bartonella spp. samples were included. Forty-three previously tested blood samples with known PCR results for hemoplasmas and Bartonella spp. were selected. The samples were split between 2 laboratories. At the first laboratory, DNA was purified and run on 2 cPCR assays for the detection of hemoplasmas and Bartonella spp...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28689893/evaluation-of-serological-and-molecular-tests-used-to-identify-toxoplasma-gondii-infection-in-pregnant-women-attended-in-a-public-health-service-in-s%C3%A3-o-paulo-state-brazil
#10
Fernando Henrique Antunes Murata, Marina Neves Ferreira, Vera Lucia Pereira-Chioccola, Lígia Cosentino Junqueira Franco Spegiorin, Cristina da Silva Meira-Strejevitch, Ricardo Gava, Aparecida Perpétuo Silveira-Carvalho, Luiz Carlos de Mattos, Cinara Cássia Brandão de Mattos
Toxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (G1=94) and with clinical suspicion of toxoplasmosis (G2=53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated...
June 17, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28502279/evidence-and-molecular-characterization-of-bartonella-spp-and-hemoplasmas-in-neotropical-bats-in-brazil
#11
P Ikeda, M C Seki, A O T Carrasco, L V Rudiak, J M D Miranda, S M M Gonçalves, E G L Hoppe, A C A Albuquerque, M M G Teixeira, C E Passos, K Werther, R Z Machado, M R André
The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil...
July 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28441969/molecular-identification-of-plasmodium-spp-and-blood-meal-sources-of-anophelines-in-environmental-reserves-on-s%C3%A3-o-lu%C3%A3-s-island-state-of-maranh%C3%A3-o-brazil
#12
Mayra Araguaia Pereira Figueiredo, Silvia Maria Di Santi, Wilson Gómez Manrique, Luiz Ricardo Gonçalves, Marcos Rogério André, Rosangela Zacarias Machado
BACKGROUND: Considering the diversity of feeding habits that females of some species of anophelines present, it is important to understand which vertebrates are part of blood food sources and how important is the role of each in the ecoepidemiology of malaria. There are many vector species for Plasmodium spp. in the State of Maranhão, Brazil. In São Luís Island, Anopheles aquasalis is the main vector for human malaria; this species is abundant in areas with primates that are positive for Plasmodium...
April 26, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28440822/a-carbonyl-reductase-from-candida-parapsilosis-atcc-7330-substrate-selectivity-and-enantiospecificity
#13
Sneha Sudhakara, Anju Chadha
Candida parapsilosis ATCC 7330, a rich source of highly stereospecific oxidoreductases, catalyzes oxidation-reduction of a plethora of compounds yielding industrially important intermediates. An (S)-specific carbonyl reductase (SRED) purified and characterized from this yeast is reported here. (R)-Specific carbonyl reductase (CpCR) was reported by us earlier. SRED asymmetrically reduces ketones with excellent enantiospecificity (ee > 99%) and α-ketoesters with higher catalytic activity but moderate enantiospecificity (ee 70%) in the presence of NADPH...
May 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28438522/detection-of-leishmania-infantum-dna-in-conjunctival-swabs-of-cats-by-quantitative-real-time-pcr
#14
Julia Cristina Benassi, Graziella U Benvenga, Helena Lage Ferreira, Vanessa F Pereira, Lara B Keid, Rodrigo Soares, Tricia Maria Ferreira de Sousa Oliveira
Although some studies have investigated the potential role of cats as a reservoir for Leishmania, their role in the epidemiology of visceral leishmaniasis (VL) is still poorly understood. Molecular diagnostic techniques are an important tool in VL diagnosis, and PCR shows high sensitivity and specificity for Leishmania spp. detection. Quantitative real-time PCR (qPCR) is a method that permits quantitative analysis of a large number of samples, resulting in more sensitive, accurate, and reproducible measurements of specific DNA present in the sample...
June 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28357075/de-novo-systemic-deleterious-amino-acid-substitutions-are-common-in-large-cytoskeleton-related-protein-coding-regions
#15
Rebecca J Stoll, Grace R Thompson, Mohammad D Samy, George Blanck
Human mutagenesis is largely random, thus large coding regions, simply on the basis of probability, represent relatively large mutagenesis targets. Thus, we considered the possibility that large cytoskeletal-protein related coding regions (CPCRs), including extra-cellular matrix (ECM) coding regions, would have systemic nucleotide variants that are not present in common SNP databases. Presumably, such variants arose recently in development or in recent, preceding generations. Using matched breast cancer and blood-derived normal datasets from the cancer genome atlas, CPCR single nucleotide variants (SNVs) not present in the All SNPs(142) or 1000 Genomes databases were identified...
February 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28268038/comparison-of-different-commercial-dna-extraction-kits-and-pcr-protocols-for-the-detection-of-echinococcus-multilocularis-eggs-in-faecal-samples-from-foxes
#16
COMPARATIVE STUDY
Pavlo Maksimov, Gereon Schares, Sebastian Press, Andreas Fröhlich, Walter Basso, Mandy Herzig, Franz J Conraths
Effective and sensitive methods for the molecular detection of Echinococcus multilocularis in faecal samples of final hosts are crucial for the prevention and control of human alveolar echinococcosis and for studies on the epidemiology of the parasite. Little is known about the suitability of commercial test kits for isolating DNA of E. multilocularis from fox faeces and the performance of standard Polymerase Chain Reaction (PCR) protocols in relation to the quality of DNA extracted by these kits. We compared four different kits: ZR Faecal DNA MiniPrep™ (Zymo Research), FastDNA(®) SPIN Kit for Soil (MP Biomedicals), QIAamp(®) Fast DNA Stool Mini Kit (QIAGEN) and NucleoSpin(®) Soil Kit (Macherey-Nagel) for the extraction of DNA from E...
April 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28262633/diagnostic-validation-of-three-test-methods-for-detection-of-cyprinid-herpesvirus-3-cyhv-3
#17
Sharon C Clouthier, Carol McClure, Tamara Schroeder, Megan Desai, Laura Hawley, Sunita Khatkar, Melissa Lindsay, Geoff Lowe, Jon Richard, Eric D Anderson
Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of koi herpesvirus disease in koi and common carp. The disease is notifiable to the World Organisation for Animal Health. Three tests-quantitative polymerase chain reaction (qPCR), conventional PCR (cPCR) and virus isolation by cell culture (VI)-were validated to assess their fitness as diagnostic tools for detection of CyHV-3. Test performance metrics of diagnostic accuracy were sensitivity (DSe) and specificity (DSp). Repeatability and reproducibility were measured to assess diagnostic precision...
March 6, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28039401/smoking-correlates-with-increased-cytoskeletal-protein-related-coding-region-mutations-in-the-lung-and-head-and-neck-datasets-of-the-cancer-genome-atlas
#18
John M Yavorski, George Blanck
Cancer from smoking tobacco is considered dependent on mutagens, but significant molecular aspects of smoking-specific, cancer development remain unknown. We defined sets of coding regions for oncoproteins, tumor suppressor proteins, and cytoskeletal-related proteins that were compared between nonsmokers and smokers, for mutation occurrences, in the lung adenocarcinoma (LUAD), head and neck squamous carcinoma (HNSC), bladder carcinoma (BLCA), and pancreatic adenocarcinoma ( PAAD) datasets from the cancer genome atlas (TCGA)...
December 2016: Physiological Reports
https://www.readbyqxmd.com/read/27876969/molecular-detection-of-giardiasis-among-children-at-cairo-university-pediatrics-hospitals
#19
Marwa A Ghieth, Magd A Kotb, Enas Y Abu-Sarea, Ayman A El-Badry
Giardiasis is a prevailing intestinal disease in children. This study aimed to determine molecular prevalence of Giardia intestinalis in children attending Cairo University Pediatrics Hospitals, using copro-PCR assays, conventional methods and to evaluate diagnostic effectiveness of used tests. 229 fecal samples were collected from children suffering from gastrointestinal symptoms and examined for Giardia by microscopy, Immuno-chromatographic test (ICT), copro-DNA using two PCR assays targeting tpi [nested-PCR (nPCR)] and 18S [conventional-PCR (cPCR)] genes...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27774854/detection-of-leishmania-l-infantum-in-stray-dogs-by-molecular-techniques-with-sensitive-species-specific-primers
#20
Rodrigo C Silva, Virgínia B Richini-Pereira, Mariana Kikuti, Pâmela M Marson, Helio Langoni
BACKGROUND: Canine visceral leishmaniasis (CVL) is a worldwide parasitic zoonosis caused by Leishmania (Leishmania) infantum around the world. Canids are the definitive hosts and sand flies the intermediate hosts. OBJECTIVE: To test the hypothesis that a new species-specific primers (Lch14:Lch15, targeting a multiple alignment for L. infantum kDNA minicircle) is an efficient diagnostic tool for L. infantum. METHODS: The presence of L. infantum DNA was assessed in blood samples of 69 stray dogs using the conventional PCR (cPCR) and quantitative PCR (qPCR)...
December 2017: Veterinary Quarterly
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