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Blood outgrowth endothelial cell

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https://www.readbyqxmd.com/read/29771429/the-relationship-between-number-and-function-of-epcs-and-concentration-of-vegf165-and-sdf-1-in-coronary-artery-spasm
#1
Z-Q Sheng, Y-F Li, K-L Zheng, H-H Lu, J Xie, H Wu, B Xu
OBJECTIVE: We aimed at investigating the functions of endothelial progenitor cells (EPCs) in the pathophysiology of coronary artery spasm (CAS) and coronary artery disease (CAD), as well as to evaluate the correlation of these diseases with the number and function of EPCs, the plasma concentration of vascular endothelial growth factor 165 (VEGF165) and stromal cell-derived factor 1 (SDF-1). PATIENTS AND METHODS: Participants were recruited into three groups, CAS, CAD and the control...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29771426/the-relationship-between-the-levels-and-function-of-endothelial-progenitor-cells-and-factor-v-leiden-and-protein-c-deficiency-in-patients-with-primary-budd-chiari-syndrome
#2
Z-Y Guan, C-W Yu, T Song, Y Gao
OBJECTIVE: Budd-Chiari syndrome (BCS) is a life-threatening hepatic disease characterized by hepatic venous obstruction at the level of hepatic vein, hepatic venules, or inferior vena cava. No evidence reported the relationship between the endothelial progenitor cells and the deficiency of factor V Leiden and protein C in patients with primary Budd-Chiari syndrome. PATIENTS AND METHODS: We recruited participants between June 2014 and July 2015. For primary BCS group, 28 patients were collected...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29749044/mycobacteria-employ-two-different-mechanisms-to-cross-the-blood-brain-barrier
#3
L M van Leeuwen, M Boot, C Kuijl, D I Picavet, G van den Brink, S M A van der Pol, H E de Vries, N N van der Wel, M van der Kuip, A M van Furth, A M van der Sar, W Bitter
Central nervous system (CNS) infection by Mycobacterium tuberculosis is one of the most devastating complications of tuberculosis, in particular in early childhood. In order to induce CNS infection, M. tuberculosis needs to cross specialized barriers protecting the brain. How M. tuberculosis crosses the blood-brain barrier (BBB) and enters the CNS is not well understood. Here, we use transparent zebrafish larvae and the closely related pathogen Mycobacterium marinum to answer this question. We show that in the early stages of development mycobacteria rapidly infect brain tissue, either as free mycobacteria or within circulating macrophages...
May 10, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29676587/a-potential-role-for-exosomal-tctp-export-in-vascular-remodeling-in-pulmonary-arterial-hypertension
#4
Elisabet Ferrer, Benjamin J Dunmore, Dhiya Hassan, Mark L Ormiston, Stephen Moore, John Deighton, Lu Long, Xu Dong Yang, Duncan J Stewart, Nicholas W Morrell
Pulmonary arterial hypertension (PAH) is characterized by increased proliferation and resistance to apoptosis of pulmonary vascular cells. Increased expression of translationally controlled tumor protein (TCTP), a pro-survival and anti-apoptotic mediator, has recently been demonstrated in patients with hereditary PAH (HPAH) although its role in the pathobiology of PAH remains unclear. Silencing of TCTP in blood outgrowth endothelial cells (BOECs) isolated from control subjects led to significant changes in morphology, cytoskeletal organization, increased apoptosis and decreased directionality during migration...
April 20, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29431053/nanoparticle-mediated-cell-capture-enables-rapid-endothelialization-of-a-novel-bare-metal-stent
#5
Brandon J Tefft, Susheil Uthamaraj, Adriana Harbuzariu, J Jonathan Harburn, Tyra A Witt, Brant Newman, Peter J Psaltis, Ota Hlinomaz, David R Holmes, Rajiv Gulati, Robert D Simari, Dan Dragomir-Daescu, Gurpreet S Sandhu
Incomplete endothelialization of intracoronary stents has been associated with stent thrombosis and recurrent symptoms, whereas prolonged use of dual antiplatelet therapy increases bleeding-related adverse events. Facilitated endothelialization has the potential to improve clinical outcomes in patients who are unable to tolerate dual antiplatelet therapy. The objective of this study was to demonstrate the feasibility of magnetic cell capture to rapidly endothelialize intracoronary stents in a large animal model...
March 13, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29371595/defining-the-role-of-the-tumor-vasculature-in-antitumor-immunity-and-immunotherapy
#6
REVIEW
Marco B Schaaf, Abhishek D Garg, Patrizia Agostinis
It is now well established that cancer cells co-exist within a complex environment with stromal cells and depend for their growth and dissemination on tight and plastic interactions with components of the tumor microenvironment (TME). Cancer cells incite the formation of new blood and lymphatic vessels from preexisting vessels to cope with their high nutrient/oxygen demand and favor tumor outgrowth. Research over the past decades has highlighted the crucial role played by tumor-associated blood and lymphatic vasculature in supporting immunoevasion and in subverting T-cell-mediated immunosurveillance, which are the main hallmarks of cancers...
January 25, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29259207/dickkopf2-rescues-erectile-function-by-enhancing-penile-neurovascular-regeneration-in-a-mouse-model-of-cavernous-nerve-injury
#7
Kalyan Ghatak, Guo Nan Yin, Min-Ji Choi, Anita Limanjaya, Nguyen Nhat Minh, Jiyeon Ock, Kang-Moon Song, Dong Hyuk Kang, Young-Guen Kwon, Ho Min Kim, Ji-Kan Ryu, Jun-Kyu Suh
Penile erection is a neurovascular event and neurologic or vascular disturbances are major causes of erectile dysfunction (ED). Radical prostatectomy for prostate cancer not only induces cavernous nerve injury (CNI) but also results in cavernous angiopathy, which is responsible for poor responsiveness to oral phosphodiesterase-5 inhibitors. Dickkopf2 (DKK2) is known as a Wnt signaling antagonist and is reported to promote mature and stable blood vessel formation. Here, we demonstrated in CNI mice that overexpression of DKK2 by administering DKK2 protein or by using DKK2-Tg mice successfully restored erectile function: this recovery was accompanied by enhanced neural regeneration through the secretion of neurotrophic factors, and restoration of cavernous endothelial cell and pericyte content...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29197873/comparative-anti-platelet-profiling-reveals-a-potent-anti-aggregatory-effect-of-cd34-progenitor-cell-derived-late-outgrowth-endothelial-cells-in-vitro
#8
Vasileios G Chantzichristos, Fani Gkrozou, Konstantinos Stellos, Minas E Paschopoulos, Alexandros D Tselepis
BACKGROUND/AIMS: Platelets affect endothelial progenitor cell (EPC) functionality, inducing their differentiation into mature endothelial cells. However, it remains to be established whether EPCs can influence platelet functionality. METHODS: Mononuclear proangiogenic cells (MPCs) and early outgrowth cells (EOCs) were prepared from peripheral blood mononuclear cells, whereas late-outgrowth endothelial cells (OECs) were prepared from cord blood CD34+ cells. The effect of the above cells and their supernatants on washed platelet aggregation was studied...
2018: Journal of Vascular Research
https://www.readbyqxmd.com/read/29058484/neurite-outgrowth-inhibitor-b-receptor-a-versatile-receptor-with-multiple-functions-and-actions
#9
REVIEW
Shuang-Lian Long, Yu-Kun Li, Yuan-Jie Xie, Zhi-Feng Long, Jin-Feng Shi, Zhong-Cheng Mo
Members of the reticulon protein family are predominantly distributed within the endoplasmic reticulum. The neurite outgrowth inhibitor (Nogo) has three subtypes, including Nogo-A (200 kDa), Nogo-B (55 kDa), and Nogo-C (25 kDa). Nogo-A and Nogo-C are potent Nogos that are predominantly expressed in the central nervous system. Nogo-B, the splice variant of reticulon-4, is expressed widely in multiple human organ systems, including the liver, lung, kidney, blood vessels, and inflammatory cells. Moreover, the Nogo-B receptor (NgBR) can interact with Nogo-B and can independently affect nervous system regeneration, the chemotaxis of endothelial cells, proliferation, and apoptosis...
December 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/29037896/manipulation-of-the-response-of-human-endothelial-colony-forming-cells-by-focal-adhesion-assembly-using-gradient-nanopattern-plates
#10
Long-Hui Cui, Hyung Joon Joo, Dae Hwan Kim, Ha-Rim Seo, Jung Suk Kim, Seung-Cheol Choi, Li-Hua Huang, Ji Eun Na, I-Rang Lim, Jong-Ho Kim, Im Joo Rhyu, Soon Jun Hong, Kyu Back Lee, Do-Sun Lim
Nanotopography plays a pivotal role in the regulation of cellular responses. Nonetheless, little is known about how the gradient size of nanostructural stimuli alters the responses of endothelial progenitor cells without chemical factors. Herein, the fabrication of gradient nanopattern plates intended to mimic microenvironment nanotopography is described. The gradient nanopattern plates consist of nanopillars of increasing diameter ranges [120-200 nm (GP 120/200), 200-280 nm (GP 200/280), and 280-360 nm (GP 280/360)] that were used to screen the responses of human endothelial colony-forming cells (hECFCs)...
January 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/28973643/tacrolimus-rescues-the-signaling-and-gene-expression-signature-of-endothelial-alk1-loss-of-function-and-improves-hht-vascular-pathology
#11
Santiago Ruiz, Pallavi Chandakkar, Haitian Zhao, Julien Papoin, Prodyot K Chatterjee, Erica Christen, Christine N Metz, Lionel Blanc, Fabien Campagne, Philippe Marambaud
Hereditary hemorrhagic telangiectasia (HHT) is a highly debilitating and life-threatening genetic vascular disorder arising from endothelial cell (EC) proliferation and hypervascularization, for which no cure exists. Because HHT is caused by loss-of-function mutations in bone morphogenetic protein 9 (BMP9)-ALK1-Smad1/5/8 signaling, interventions aimed at activating this pathway are of therapeutic value. We interrogated the whole-transcriptome in human umbilical vein ECs (HUVECs) and found that ALK1 signaling inhibition was associated with a specific pro-angiogenic gene expression signature, which included a significant elevation of DLL4 expression...
December 15, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28971999/identification-of-microrna-124-as-a-major-regulator-of-enhanced-endothelial-cell-glycolysis-in-pulmonary-arterial-hypertension-via-ptbp1-polypyrimidine-tract-binding-protein-and-pyruvate-kinase-m2
#12
Paola Caruso, Benjamin J Dunmore, Kenny Schlosser, Sandra Schoors, Claudia Dos Santos, Carol Perez-Iratxeta, Jessie R Lavoie, Hui Zhang, Lu Long, Amanda R Flockton, Maria G Frid, Paul D Upton, Angelo D'Alessandro, Charaka Hadinnapola, Fedir N Kiskin, Mohamad Taha, Liam A Hurst, Mark L Ormiston, Akiko Hata, Kurt R Stenmark, Peter Carmeliet, Duncan J Stewart, Nicholas W Morrell
BACKGROUND: Pulmonary arterial hypertension (PAH) is characterized by abnormal growth and enhanced glycolysis of pulmonary artery endothelial cells. However, the mechanisms underlying alterations in energy production have not been identified. METHODS: Here, we examined the miRNA and proteomic profiles of blood outgrowth endothelial cells (BOECs) from patients with heritable PAH caused by mutations in the bone morphogenetic protein receptor type 2 ( BMPR2 ) gene and patients with idiopathic PAH to determine mechanisms underlying abnormal endothelial glycolysis...
December 19, 2017: Circulation
https://www.readbyqxmd.com/read/28963481/synthetic-microparticles-conjugated-with-vegf-165-improve-the-survival-of-endothelial-progenitor-cells-via-microrna-17-inhibition
#13
Sezin Aday, Janet Zoldan, Marie Besnier, Laura Carreto, Jaimy Saif, Rui Fernandes, Tiago Santos, Liliana Bernardino, Robert Langer, Costanza Emanueli, Lino Ferreira
Several cell-based therapies are under pre-clinical and clinical evaluation for the treatment of ischemic diseases. Poor survival and vascular engraftment rates of transplanted cells force them to work mainly via time-limited paracrine actions. Although several approaches, including the use of soluble vascular endothelial growth factor (sVEGF)-VEGF165 , have been developed in the last 10 years to enhance cell survival, they showed limited efficacy. Here, we report a pro-survival approach based on VEGF-immobilized microparticles (VEGF-MPs)...
September 29, 2017: Nature Communications
https://www.readbyqxmd.com/read/28956952/role-of-the-aryl-hydrocarbon-receptor-in-sugen-5416-induced-experimental-pulmonary-hypertension
#14
Afshan Dean, Teja Gregorc, Craig K Docherty, Katie Y Harvey, Margaret Nilsen, Nicholas W Morrell, Margaret R MacLean
Rats dosed with the vascular endothelial growth factor inhibitor Sugen 5416 (Su), subjected to hypoxia, and then restored to normoxia have become a widely used model of pulmonary arterial hypertension (PAH). However, the mechanism by which Su exacerbates pulmonary hypertension is unclear. We investigated Su activation of the aryl hydrocarbon receptor (AhR) in human pulmonary artery smooth muscle cells (hPASMCs) and blood outgrowth endothelial cells (BOECs) from female patients with PAH. We also examined the effect of AhR on aromatase and estrogen levels in the lung...
March 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28927082/identification-of-target-gene-of-venous-thromboembolism-in-patients-with-lymphoma-via-microarray-analysis
#15
Pengfei Liu, Wenhua Jiang, Huilai Zhang
Patients with lymphoma are at high risk of developing venous thromboembolism (VTE). The purpose of the present study was to identify the target gene associated with VTE for patients with lymphoma. Microarray data was downloaded from the gene expression omnibus database (GSE17078), which comprised the control group, 27 normal blood outgrowth endothelial cell (BOEC) samples, and the case group, 3 BOEC samples of venous thrombosis with protein C deficiency. Differentially expressed genes (DEGs) were identified by the Limma package of R...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28910385/improving-the-characterization-of-endothelial-progenitor-cell-subsets-by-an-optimized-facs-protocol
#16
Karin Huizer, Dana A M Mustafa, J Clarissa Spelt, Johan M Kros, Andrea Sacchetti
The characterization of circulating endothelial progenitor cells (EPCs) is fundamental to any study related to angiogenesis. Unfortunately, current literature lacks consistency in the definition of EPC subsets due to variations in isolation strategies and inconsistencies in the use of lineage markers. Here we address critical points in the identification of hematopoietic progenitor cells (HPCs), circulating endothelial cells (CECs), and culture-generated outgrowth endothelial cells (OECs) from blood samples of healthy adults (AB) and umbilical cord (UCB)...
2017: PloS One
https://www.readbyqxmd.com/read/28875579/a-cardiac-patch-from-aligned-microvessel-and-cardiomyocyte-patches
#17
Jeremy A Schaefer, Pilar A Guzman, Sonja B Riemenschneider, Timothy J Kamp, Robert T Tranquillo
Cardiac tissue engineering aims to produce replacement tissue patches in the lab to replace or treat infarcted myocardium. However, current patches lack preformed microvascularization and are therefore limited in thickness and force production. In this study, we sought to assess whether a bilayer patch composed of a layer made from human induced pluripotent stem cell-derived cardiomyocytes and a microvessel layer composed of self-assembled human blood outgrowth endothelial cells and pericytes was capable of engrafting on the epicardial surface of a nude rat infarct model and becoming perfused by the host 4 weeks after acute implantation...
February 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28875559/alginate-hydrogels-of-varied-molecular-weight-distribution-enable-sustained-release-of-sphingosine-1-phosphate-and-promote-angiogenesis
#18
Priscilla A Williams, Kevin T Campbell, Eduardo A Silva
Alginate hydrogels have been widely validated for controlled release of growth factors and cytokines, but studies exploring sustained release of small hydrophobic lipids are lacking. Sphingosine-1-phosphate (S1P), a bioactive lipid, is an appealing small molecule for inducing blood vessel formation in the context of ischemic conditions. However, there are numerous biological and engineering challenges associated with designing biomaterial systems for controlled release of this lipid. Thus, the objective of this study was to design an injectable, alginate hydrogel formulation that provides controlled release of S1P to establish locally sustained concentration gradients that promote neovascularization...
January 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28632184/crude-fucoidan-extracts-impair-angiogenesis-in-models-relevant-for-bone-regeneration-and-osteosarcoma-via-reduction-of-vegf-and-sdf-1
#19
Fanlu Wang, Harald Schmidt, Dijana Pavleska, Thees Wermann, Andreas Seekamp, Sabine Fuchs
The marine origin polysaccharide fucoidan combines multiple biological activities. As demonstrated by various studies in vitro and in vivo, fucoidans show anti-viral, anti-tumor, anti-oxidant, anti-inflammatory and anti-coagulant properties, although the detailed molecular action remains to be elucidated. The aim of the present study is to assess the impact of crude fucoidan extracts, on the formation of vascular structures in co-culture models relevant for bone vascularization during bone repair and for vascularization processes in osteosarcoma...
June 20, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28546532/regulation-of-c-jun-n-terminal-protein-kinase-jnk-pathway-in-apoptosis-of-endothelial-outgrowth-cells-induced-by-asymmetric-dimethylarginine
#20
Fu-Qing Zhang, Wei Lu, Wen-Xiao Yuan, Xin Li
BACKGROUND Endothelial outgrowth cells (EOCs) are terminal endothelial progenitor cells (EPCs). Asymmetric dimethylarginine (ADMA) has been identified as a novel risk factor for cardiovascular diseases. Our aim in the present study was to investigate the effect of regulation of asymmetric dimethylarginine (ADMA) on EOCs apoptosis and to explore the underlining mechanisms of c-Jun N-terminal protein kinase (JNK) pathway in the process. MATERIAL AND METHODS EOCs were harvested from umbilical cord blood and obtained by using density gradient centrifugation and adhesive culture methods...
May 26, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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