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https://www.readbyqxmd.com/read/28445252/irreversible-electroporation-in-primary-and-metastatic-hepatic-malignancies-a-review
#1
Tianchu Lyu, Xifu Wang, Zhanliang Su, Junjie Shangguan, Chong Sun, Matteo Figini, Jian Wang, Vahid Yaghmai, Andrew C Larson, Zhuoli Zhang
BACKGROUND: Liver cancer makes up a huge percentage of cancer mortality worldwide. Irreversible electroporation (IRE) is a relatively new minimally invasive nonthermal ablation technique for tumors that applies short pulses of high frequency electrical energy to irreversibly destabilize cell membrane to induce tumor cell apoptosis. METHODS: This review aims to investigate the studies regarding the use of IRE treatment in liver tumors and metastases to liver. We searched PubMed for all of IRE relevant English language articles published up to September 2016...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28445136/selective-eradication-of-cancer-cells-by-delivery-of-adenovirus-based-toxins
#2
Shiran Shapira, Assaf Shapira, Diana Kazanov, Gil Hevroni, Sarah Kraus, Nadir Arber
BACKGROUND AND OBJECTIVE: KRAS mutation is an early event in colorectal cancer carcinogenesis. We previously reported that a recombinant adenovirus, carrying a pro-apoptotic gene (PUMA) under the regulation of Ets/AP1 (RAS-responsive elements) suppressed the growth of cancer cells harboring hyperactive KRAS. We propose to exploit the hyperactive RAS pathway, rather than to inhibit it as was previously tried and failed repeatedly. We aim to improve efficacy by substituting PUMA with a more potent toxin, the bacterial MazF-MazE toxin-antitoxin system, under a very tight regulation...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28442328/simple-quick-and-cost-efficient-a-universal-rt-pcr-and-sequencing-strategy-for-genomic-characterisation-of-foot-and-mouth-disease-viruses
#3
V Dill, M Beer, B Hoffmann
Foot-and-mouth disease (FMD) is a major contributor to poverty and food insecurity in Africa and Asia, and it is one of the biggest threats to agriculture in highly developed countries. As FMD is extremely contagious, strategies for its prevention, early detection, and the immediate characterization of outbreak strains are of great importance. The generation of whole-genome sequences enables phylogenetic characterization, the epidemiological tracing of virus transmission pathways and is supportive in disease control strategies...
April 22, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28433771/a-versatile-tumor-gene-deletion-system-reveals-a-crucial-role-for-fgfr1-in-breast-cancer-metastasis
#4
Wei Wang, Yanling Meng, Bingning Dong, Jie Dong, Michael M Ittmann, Chad J Creighton, Lu Yang, Hong Zhang, Tao Shen, Jianghua Wang, David R Rowley, Yi Li, Fengju Chen, David D Moore, Feng Yang
RCAS avian viruses have been used to deliver oncogene expression and induce tumors in transgenic mice expressing the virus receptor TVA. Here we report the generation and characterization of a novel RCAS-Cre-IRES-PyMT (RCI-PyMT) virus designed to specifically knockout genes of interest in tumors generated in appropriate mutant mouse hosts. FGF receptor 1 (FGFR1) is a gene that is amplified in human breast cancer, but there have been no definitive studies on its function in mammary tumorigenesis, progression, and metastasis in vivo in spontaneous tumors in mice...
April 20, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28423496/mtor-inhibitors-activate-perk-signaling-and-favor-viability-of-gastrointestinal-neuroendocrine-cell-lines
#5
Patricia Freis, Julien Bollard, Justine Lebeau, Patrick Massoma, Joëlle Fauvre, Cécile Vercherat, Thomas Walter, Serge Manié, Colette Roche, Jean-Yves Scoazec, Carole Ferraro-Peyret
mTOR and Unfolded Protein Response (UPR) are two signaling pathways frequently activated in cancer cells. The mTOR pathway has been shown to be up-regulated in most gastroenteropancreatic neuroendocrine tumors. In contrast, little is known about the UPR status in neoplastic neuroendocrine cells. However, these hormone-producing cells are likely to present distinctive adaptations of this pathway, as other secretory cells. We therefore analyzed the status of the three axes of UPR and their relation to mTOR pathway in two gastrointestinal neuroendocrine tumors (GI-NET) cell lines STC-1 and GluTag...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420742/synergistic-internal-ribosome-entry-site-microrna-based-approach-for-flavivirus-attenuation-and-live-vaccine-development
#6
Konstantin A Tsetsarkin, Guangping Liu, Evgeniya Volkova, Alexander G Pletnev
The recent emergence of Zika virus underscores the need for new strategies for a rapid development of safe flavivirus vaccines. Using another flavivirus (Langat virus [LGTV]) that belongs to the group of tick-borne flaviviruses as a model, we describe a dual strategy for virus attenuation which synergistically accesses the specificity of microRNA (miRNA) genome targeting and the effectiveness of internal ribosome entry site (IRES) insertion. To increase the stability and immunogenicity of bicistronic LGTVs, we developed a novel approach in which the capsid (C) protein gene was relocated into the 3' noncoding region (NCR) and expressed under translational control from an IRES...
April 18, 2017: MBio
https://www.readbyqxmd.com/read/28417251/irreversible-electroporation-for-the-treatment-of-rabbit-vx2-breast-cancer
#7
Wenlong Zhang, Wei Chai, Jianying Zeng, Jibing Chen, Liqi Bi, Lizhi Niu
Irreversible electroporation (IRE) is considered predominantly as a non-thermal ablative technique that uses electrical fields to permeabilize cell membranes and lead to cell death. In this study, we evaluated the efficacy of IRE in the rabbit VX2 breast cancer model. Thirty-five female New Zealand white rabbits were inoculated against VX2 breast cancer cells. The rabbits were randomly divided into two groups: a control group of 15 rabbits and an IRE treatment group of 20 rabbits. Treatment and treatment outcome were evaluated by computerized tomography (CT) scan (plain or contrast enhanced), tumor growth curves and pathological examination including H&E, TUNEL, PCNA and CD31 staining...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28414500/location-coding-account-versus-affordance-activation-account-in-handle-to-hand-correspondence-effects-evidence-of-simon-like-effects-based-on-the-coding-of-action-direction
#8
Antonello Pellicano, Iring Koch, Ferdinand Binkofski
An increasing number of studies have shown a close link between perception and action, which is supposed to be responsible for the automatic activation of actions compatible with objects' properties, such as the orientation of their graspable parts. It has been observed that left and right hand responses to objects (e.g., cups) are faster and more accurate if the handle orientation corresponds to the response location than when it does not. Two alternative explanations have been proposed for this handle-to-hand correspondence effect: location coding and affordance activation...
April 17, 2017: Journal of Experimental Psychology. Human Perception and Performance
https://www.readbyqxmd.com/read/28400295/phic31-integrase-can-improve-the-efficiency-of-different-construct-designs-for-monoclonal-antibody-expression-in-cho-cells
#9
Maryam Ahmadi, Fereidoun Mahboudi, Samira Ahmadi, Saeedeh Ebadat, Fatemeh Nematpour, Mohammad Reza Akbari Eidgahi, Fatemeh Davami
OBJECTIVES: Several types of expression vectors have been used for recombinant protein expression in Chinese hamster ovary cells (CHO) which usually result in variable and unstable levels of expression. METHODS AND RESULTS: In this study, we have compared the mAb0014 expression level of single ORF/IRES vector and dual ORF vector in the presence and absence of phiC31 integrase targeting system. Both expression vectors contain an elongation factor 1α (EF1α) promoter upstream of LC and harboring an attB site...
April 9, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28397218/-generation-of-gabaergic-interneuron-specific-pgc-1%C3%AE-knockout-mice
#10
Jia Wang, Xianlu Chang, Jinjun Qian, Yang Jiang, Chunyan Wang, Yucong Wang, Chi Feng, Yongjie Wang, Peihui Xia, Ying Zhang, Danping Wu, Weining Zhang
OBJECTIVE: To generate mice which are specific for peroxisomproliferator-activated receptor-γ coactivator-1(PGC-1α) knockout in the GABAergic interneuron. METHODS: Conditional mice specific for PGC-1α(f/+) were introduced from the Jackson Laboratory, USA and initially inbred to obtain homozygote PGC-1α (f/f) mice. The PGC-1α(f/f) conditional mice were further crossed with Dlx5/6-Cre-IRES-EGFP transgenic mice to achieve specific knockout of PGC-1α in the GABAergic interneuron...
April 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28395113/a-novel-role-for-crtc2-in-hepatic-cholesterol-synthesis-via-srebp-2
#11
Yujie Li, Yongfeng Song, Meng Zhao, Yanjing Guo, Chunxiao Yu, Wenbin Chen, Shanshan Shao, Chao Xu, Xinli Zhou, Lifang Zhao, Zhenhai Zhang, Tao Bo, Yu Xia, Christopher G Proud, Xuemin Wang, Li Wang, Jiajun Zhao, Ling Gao
Cholesterol synthesis is regulated by the transcription factor SREBP-2 and its target gene 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), which is the rate-limiting enzyme in cholesterol synthesis. CREB-regulated transcription coactivator (CRTC) 2 is the master regulator of glucose metabolism. However, the effect of CRTC2 on cholesterol and its potential molecular mechanism remain unclear. Here, we demonstrated that CRTC2 expression and the liver cholesterol content were increased in patients with high serum cholesterol levels who underwent resection of liver hemangiomas, as well as in mice fed a 4% cholesterol diet...
April 10, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28392199/short-term-clinical-efficacy-of-percutaneous-irreversible-electroporation-combined-with-allogeneic-natural-killer-cell-for-treating-metastatic-pancreatic-cancer
#12
Mao Lin, Shuzhen Liang, Xiaohua Wang, Yinqing Liang, Mingjie Zhang, Jibing Chen, Lizhi Niu, Kecheng Xu
This study was to determine how the short-term clinical efficacy of irreversible electroporation (IRE) combined with allogeneic natural killer (NK) cell therapy for treating metastatic pancreatic cancer. Between March and December 2016, we enrolled 40 patients who met the enrollment criteria and assigned them to two groups: simple IRE (IRE group, n=20) and IRE plus allogeneic NK cell therapy (IRE-NK, n=20). We evaluated immune function changes, quality of life, clinical response, and other related indicators...
April 6, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28391185/bipolar-microsecond-pulses-and-insulated-needle-electrodes-for-reducing-muscle-contractions-during-irreversible-electroporation
#13
Chenguo Yao, Shoulong Dong, Yajun Zhao, Yanpeng Lv, Hongmei Liu, Lingyu Gong, Jianhao Ma, Haifeng Wang, Yinghao Sun
To minimize the effect of muscle contractions during irreversible electroporation (IRE), this paper attempts to research the ablation effect and muscle contractions by applying high-frequency irreversible electroporation (H-FIRE) ablation to liver tissue in vivo. METHODS: Insulated needle electrode was designed by painting insulating-coating on the outer surface of the needle electrode tip. Series experiments were conducted using insulated needle electrodes and traditional needle electrodes to apply H-FIRE pulses and traditional monopolar IRE pulses to rabbit liver tissues...
April 4, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28390865/spdef-induces-quiescence-of-colorectal-cancer-cells-by-changing-the-transcriptional-targets-of-%C3%AE-catenin
#14
Yuan-Hung Lo, Taeko K Noah, Min-Shan Chen, Winnie Zou, Ester Borras, Eduardo Vilar, Noah F Shroyer
BACKGROUND & AIMS: The canonical Wnt signaling pathway activates the transcriptional activity of β-catenin. This pathway is often activated in colorectal cancer cells, but strategies to block it in tumors have not been effective. The SAM pointed domain containing ETS transcription factor (SPDEF) suppresses formation of colon tumors by unclear mechanisms. We investigated these mechanisms and the effects of SPDEF on β-catenin activity in mouse models of colorectal cancer (CRC), CRC cell lines, and mouse and human normal and cancer colonoids...
April 5, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28386934/eomes-expression-reports-the-progressive-differentiation-of-ifn-%C3%AE-producing-th1-like-%C3%AE-%C3%AE-t%C3%A2-cells
#15
Ciro N R Lino, Joana Barros-Martins, Linda Oberdörfer, Thierry Walzer, Immo Prinz
The transcription factor Eomesodermin (Eomes) plays a crucial role in regulating cytotoxic function, development, and survival of immune cells. γδ T cells can express Eomes, but its contribution to their differentiation is unknown. Using Eomes-IRES-GFP mice, we show that Eomes(+) γδ T cells are unequally distributed among organs, with the highest proportion in spleen. While the majority of Eomes(+) γδ T cells expressed Vγ1(+) and Vγ4(+) TCRs, Eomes was absent in Vγ5(+) , Vγ6(+) , and Vγ7(+) subsets...
April 6, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28378237/successful-ablation-of-lymph-nodes-using-irreversible-electroporation-ire-in-a-porcine-survival-model
#16
Stefan Fritz, Christof M Sommer, Thomas Longerich, Clemens Kuhn-Neureuther, Boris Radeleff, Jens Werner, Thilo Hackert
BACKGROUND: Irreversible electroporation (IRE), a nonthermal injury ablation technique, has been shown to be effective and safe in various organs, such as in the kidney, liver, prostate, or in pancreas. In contrast to radiofrequency or microwave ablation, IRE is also effective in the neighborhood of major vessels. Many human cancers reveal lymphatic spread. The present study aimed to evaluate technical feasibility and safety of IRE in lymphatic tissue. To our knowledge, this is the first report showing successful IRE of lymph nodes in a standardized porcine survival model...
April 4, 2017: Langenbeck's Archives of Surgery
https://www.readbyqxmd.com/read/28366657/normal-porcine-ureter-retains-lumen-wall-integrity-but-not-patency-following-catheter-directed-irreversible-electroporation-imaging-and-histologic-assessment-over-28-days
#17
Govindarajan Srimathveeravalli, Francois Cornelis, Thomas Wimmer, Sebastien Monette, Simon Y Kimm, Majid Maybody, Stephen B Solomon, Jonathan A Coleman, Jeremy C Durack
PURPOSE: To evaluate the effect of catheter-directed irreversible electroporation (IRE) on the integrity, patency, and function of the normal porcine ureter. MATERIALS AND METHODS: A catheter-mounted electrode was used to perform fluoroscopy-guided IRE in 8 healthy pigs. Two unilateral ablations (90 pulses at 2,000 V, 100 μs) were performed in each animal in the proximal and distal ureter. Serum creatinine measurements and contrast-enhanced computed tomography imaging were performed at 1, 7, 14, 21, and 28 days after IRE, and findings were compared with baseline values by Student t test...
March 30, 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/28365947/irreversible-electroporation-nanoknife%C3%A2-treatment-in-the-field-of-hepatobiliary-surgery-current-status-and-future-perspectives
#18
Georgios Kourounis, Patrick Paul Tabet, Demetrios Moris, Alexandros Papalambros, Evangelos Felekouras, Fanourios Georgiades, George Astras, Athanasios Petrou
PURPOSE: We aimed to provide an overview of current understanding on the potential use of irreversible electroporation (IRE) in the field of hepatobiliary surgery with a focus on current results in hepatic and pancreatic cancers, its limitations, and its current directions. METHODS: Through a review of the literature we have gathered the key articles and trials that are shaping our understanding of the current status of IRE and its prospective uses, and organized them in an easily understandable format showcasing the most up to date results...
January 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28360118/raising-the-pressure-hemodynamic-effects-of-splanchnic-nerve-stimulation
#19
Marat Fudim, Suraj Yalamuri, J Taylor Herbert, Peter R Liu, Manesh R Patel, Aaron Sandler
A number of cardiovascular and neurological diseases are characterized by a dysregulation of intravascular volume distribution. The veins and arteries of the visceral organs form the so-called splanchnic vascular compartment and are the largest reservoir for intravascular blood. The blood localized in the splanchnic compartment can be mobilized in and out of the compartment via passive compression or active neurohormonal recruitment. We studied the hemodynamic effects of splanchnic nerve stimulation during five cases of Irreversible Electroporation (IRE) in patients with pancreatic cancer...
March 30, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28358866/phenotypically-silent-cre-recombination-within-the-postnatal-ventricular-conduction-system
#20
Samadrita Bhattacharyya, Minoti Bhakta, Nikhil Vilas Munshi
The cardiac conduction system (CCS) is composed of specialized cardiomyocytes that initiate and maintain cardiac rhythm. Any perturbation to the normal sequence of electrical events within the heart can result in cardiac arrhythmias. To understand how cardiac rhythm is established at the molecular level, several genetically modified mouse lines expressing Cre recombinase within specific CCS compartments have been created. In general, Cre driver lines have been generated either by homologous recombination of Cre into an endogenous locus or Cre expression driven by a randomly inserted transgene...
2017: PloS One
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