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https://www.readbyqxmd.com/read/28926103/fam20c-regulates-osteoblast-behaviors-and-intracellular-signaling-pathways-in-a-cell-autonomous-manner
#1
Chao Liu, Hua Zhang, Priyam Jani, Xiaofang Wang, Yongbo Lu, Nan Li, Jing Xiao, Chunlin Qin
Recent studies indicate that Family with sequence similarity 20 member C (FAM20C) catalyzes the phosphorylation of secreted proteins, and participates in a variety of biological processes, including cell proliferation, migration, mineralization and phosphate homeostasis. To explore the local influences of FAM20C on osteoblast, Fam20c-deficient osteoblasts were generated by treating the immortalized Fam20c(f/f) osteoblasts with CMV-Cre-IRES-EGFP lentivirus. Compared with the normal Fam20c(f/f) osteoblasts, the expression of Bone sialoprotein (Bsp), Osteocalcin (Ocn), Fibroblast growth factor 23 (Fgf23) and transcription factors that promote osteoblast maturation were up-regulated in the Fam20c-deficient osteoblasts...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28924200/controllable-moderate-heating-enhances-the-therapeutic-efficacy-of-irreversible-electroporation-for-pancreatic-cancer
#2
Chelsea M Edelblute, James Hornef, Niculina I Burcus, Thomas Norman, Stephen J Beebe, Karl Schoenbach, Richard Heller, Chunqi Jiang, Siqi Guo
Irreversible electroporation (IRE) as a non-thermal tumor ablation technology has been studied for the treatment of pancreatic carcinoma and has shown a significant survival benefit. We discovered that moderate heating (MH) at 43 °C for 1-2 minutes significantly enhanced ex vivo IRE tumor ablation of Pan02 cells by 5.67-fold at 750 V/cm and by 1.67-fold at 1500 V/cm. This amount of heating alone did not cause cell death. An integrated IRE system with controllable laser heating and tumor impedance monitoring was developed to treat mouse ectopic pancreatic cancer...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28922394/sequence-features-of-viral-and-human-internal-ribosome-entry-sites-predictive-of-their-activity
#3
Alexey A Gritsenko, Shira Weingarten-Gabbay, Shani Elias-Kirma, Ronit Nir, Dick de Ridder, Eran Segal
Translation of mRNAs through Internal Ribosome Entry Sites (IRESs) has emerged as a prominent mechanism of cellular and viral initiation. It supports cap-independent translation of select cellular genes under normal conditions, and in conditions when cap-dependent translation is inhibited. IRES structure and sequence are believed to be involved in this process. However due to the small number of IRESs known, there have been no systematic investigations of the determinants of IRES activity. With the recent discovery of thousands of novel IRESs in human and viruses, the next challenge is to decipher the sequence determinants of IRES activity...
September 18, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28922183/parental-awareness-of-schoolbag-carriage-a-comparative-study-of-irish-and-united-states-parents
#4
Sara Dockrell, Karen Jacobs, Julie Byrne, Emma Gleeson, Sinead Kelly, Caitriona Moore, Eimear O'Meara, Dagny Barclay, Sabrina Rodriguez, David Kang, Ciaran Simms, Catherine Blake
BACKGROUND: Given the global nature of schoolbag carriage, there has been extensive research on schoolbag weight and use with resultant guidance on many aspects of carrying a schoolbag. However, there is limited evidence of knowledge translation or parents' awareness of schoolbag carriage. OBJECTIVE: This study investigated parental awareness of factors related to schoolbag carriage. METHOD: A cross-sectional survey using an anonymous 30-item questionnaire and purposive sampling was used...
September 14, 2017: Work: a Journal of Prevention, Assessment, and Rehabilitation
https://www.readbyqxmd.com/read/28920152/sentinel-node-navigation-surgery-for-early-gastric-cancer-analysis-of-factors-which-affect-direction-of-lymphatic-drainage
#5
Atsuo Shida, Norio Mitsumori, Shuichi Fujioka, Yuta Takano, Muneharu Fujisaki, Ryosuke Hashizume, Naoto Takahashi, Yoshio Ishibashi, Katsuhiko Yanaga
BACKGROUND AND PURPOSE: We started performing sentinel node navigation surgery (SNNS) for patients with early gastric cancer (EGC) using infrared ray electronic endoscopy (IREE) with indocyanine green injection from year 2000. The EGCs usually have complex lymphatic drainage, unidirectional or multidirectional lymphatic flow. In this study, we investigated and clarified factors that affect the direction of gastric lymphatic drainage. PATIENTS AND METHOD: Consecutive 60 patients with EGC who underwent SNNS by IREE from year 2006 to 2014 were enrolled to this study...
September 18, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28918745/coupling-of-translation-initiation-and-termination-does-not-depend-on-the-mode-of-initiation
#6
E A Sogorin, G K Selikhanov, S Ch Agalarov
Recently we described a novel phenomenon observed during eukaryotic translation in a cell-free system: the coupling of initiation and termination on different mRNA molecules. Here we show that the phenomenon does not depend on a special mode of initiation. The mRNAs with certain leader sequences known to require different determinants for successful initiation were examined. Even in a case of using the intergenic internal ribosome entry site (IRES) of cricket paralysis virus RNA as the leader sequence, while no initiation factors are required, the effect of coupling is well expressed, including trials in the presence of hippuristanol as an inhibitor of eIF4A...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28918467/anisodamine-inhibits-endoplasmic-reticulum-stress-associated-txnip-nlrp3-inflammasome-activation-in-rhabdomyolysis-induced-acute-kidney-injury
#7
Xiaopeng Yuan, Yitao Zheng, Chuanbao Chen, Changxi Wang
Anisodamine protects against free radical-induced cellular damage. This study aimed to investigate the protective effect of anisodamine on rhabdomyolysis-induced acute kidney injury (RIAKI). C57BL/6 J mice, TXNIP(-/-) and NLRP3 (-/-) (both were C57BL/6 J background) mice were used to construct RIAKI model. Anisodamine administration was performed on RIAKI mice only. Mice were divided into control, TXNIP-KD (knock down), LNPR3-KD, and anisodamine group (n = 15 in each group). The renal injury, renal function, renal tubular cells apoptosis and expression of Caspase-1, ASC, endoplasmic reticulum (ER) stress markers IRE-1α, CHOP, and ATF4, and interleukin (IL-1α, IL-1β, and IL-18) were detected...
September 16, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28916474/nox4-regulates-the-enos-uncoupling-process-in-aging-endothelial-cells
#8
Hwa-Young Lee, Hyung-Ryong Kim, Han-Jung Chae
ROS and its associated signaling contribute to vascular aging-associated endothelial disturbance. Since the non-effective endothelial nitric oxide synthase (eNOS) coupling status is related to vascular aging-related phenotypes, eNOS coupled/uncoupled system signaling was studied in human umbilical vein endothelial cells (HUVEC). Nitric oxide (NO) and eNOS Ser1177 were significantly decreased, whereas O2(-) (superoxide anion radical) increased with passage number. In aging cells, NADPH oxidase 4 (Nox4), one of the main superoxide generating enzymes, and its associated protein disulfide isomerase (PDI) chaperone were highly activated, and the resultant ER redox imbalance leads to disturbance of protein folding capability, namely endoplasmic reticulum (ER) stress, ultimately inducing dissociation between HSP90 and IRE-1α or PERK, decreasing HSP90 stability and dissociating the binding of eNOS from the HSP90 and leading to eNOS uncoupling...
September 12, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28914820/indoor-positioning-system-based-on-a-psd-detector-precise-positioning-of-agents-in-motion-using-aoa-techniques
#9
David Rodríguez-Navarro, José Luis Lázaro-Galilea, Álvaro De-La-Llana-Calvo, Ignacio Bravo-Muñoz, Alfredo Gardel-Vicente, Georgios Tsirigotis, Juan Iglesias-Miguel
Here, we present an indoor positioning system (IPS) for detecting mobile agents based on a single Position Sensitive Device sensor (PSD) sited in the environment and InfraRed Emitter Diode (IRED) located on mobile agents. The main goal of the work is to develop an alternative IPS to other sensing technologies, cheaper, easier to install and with a low computational load to obtain a high rate of measurements per second. The proposed IPS has the capacity to accurately determine 3D position from the angle of arrival (AoA) of the signal received at the PSD sensor...
September 15, 2017: Sensors
https://www.readbyqxmd.com/read/28911115/the-ires5-utr-of-the-dicistrovirus-cricket-paralysis-virus-is-a-type-iii-ires-containing-an-essential-pseudoknot-structure
#10
Lauriane Gross, Quentin Vicens, Evelyne Einhorn, Audrey Noireterre, Laure Schaeffer, Lauriane Kuhn, Jean-Luc Imler, Gilbert Eriani, Carine Meignin, Franck Martin
Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of non-structural proteins encoded by ORF1 whereas the well-known intergenic region (IGR) IRESIGR is required for translation of structural proteins from open reading frame 2 in the late phase of infection. Concerted action of both IRES is essential for host translation shut-off and viral translation...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28885207/effect-of-irreversible-electroporation-of%C3%A2-prostate-cancer-on-microcirculation-imaging-findings-in-contrast-enhanced-t1-weighted-3d-mri
#11
L P Beyer, B Pregler, N Verloh, K Brünn, M Haimerl, C Stroszczynski, E M Jung, P Wiggermann
OBJECTIVES: Irreversible electroporation (IRE) is a novel focal ablation technique applicable for treatment of prostate cancer (PCa). We aim to evaluate imaging findings of T1-weighted contrast-enhanced MRI after percutaneous IRE of low-risk PCa. METHODS: A total of 13 male patients underwent IRE of focal low-risk PCa and were included in this analysis. Prostate IRE was conducted using 2-4 electrodes being placed under CT-fluoroscopy guidance. Dynamic contrast-enhanced 3D isotropic fat-saturated T1-weighted MRI (DCE-MRI) was performed 24-72 hours before and 24-72 hours after ablation...
September 4, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28878295/garcinone-e-induces-apoptosis-and-inhibits-migration-and-invasion-in-ovarian-cancer-cells
#12
Xiao-Huang Xu, Qian-Yu Liu, Ting Li, Jian-Lin Liu, Xin Chen, Li Huang, Wen-An Qiang, Xiuping Chen, Yitao Wang, Li-Gen Lin, Jin-Jian Lu
Ovarian cancer remains the most lethal gynecological malignant tumor. In this study, 24 xanthones were isolated and identified from the pericarps of mangosteen (Garcinia mangostana), and their anti-proliferative activities were tested in ovarian cancer cells. Garcinone E (GE) was found to exhibit excellent anti-proliferative effects among the tested xanthones. It significantly inhibited the proliferation in HEY, A2780, and A2780/Taxol cells as evidenced by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release assay, Hoechst 33342 staining, annexin V/PI staining, and JC-1 staining...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28874056/ceruloplasmin-a-potential-therapeutic-agent-for-alzheimer-s-disease
#13
Ya-Shuo Zhao, Li-Hong Zhang, Pan-Pan Yu, Yu-Jing Gou, Jing Zhao, Lin-Hao You, Zhan-You Wang, Xin Zheng, Liang-Jun Yan, Peng Yu, Yan-Zhong Chang
AIMS: Ceruloplasmin (CP), as a ferrous oxidase enzyme, plays an important role in regulating iron metabolism and redox reactions. Previous studies showed that CP deficiency contributes to Parkinson's disease by increasing iron accumulation and oxidative stress in the substantia nigra. However, the role of CP in Alzheimer's disease (AD) is unclear. We hypothesized that the lack of CP gene expression would affect the pathogenesis and damage of AD by promoting abnormal iron levels and oxidative stress...
September 6, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28871458/percutaneous-irreversible-electroporation-combined-with-allogeneic-natural-killer-cell-immunotherapy-for-patients-with-unresectable-stage-iii-iv-pancreatic-cancer-a-promising-treatment
#14
Mao Lin, Shuzhen Liang, Xiaohua Wang, Yinqing Liang, Mingjie Zhang, Jibing Chen, Lizhi Niu, Kecheng Xu
PURPOSE: This study was attempted to investigate the safety and clinical efficacy of percutaneous irreversible electroporation combined with allogeneic natural killer cell therapy for treating stage III/IV pancreatic cancer, evaluate median progression-free survival (PFS), and overall survival (OS). METHODS: Between March 2016 and February 2017, we enrolled 67 patients who met the enrollment criteria. According to the latest NCCN Guidelines, the patients were divided into stage III (35 patients, 16 patients received only irreversible electroporation (IRE) and 19 patients received IRE-NK: 8 patients underwent one course NK and 11 patients underwent ≥3 courses) and stage IV (32 patients, 14 patients received only IRE and 18 patients received IRE-NK: 8 patients underwent one course NK and 10 patients underwent ≥3 courses)...
September 4, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28871138/predicting-irreversible-electroporation-induced-tissue-damage-by-means-of-magnetic-resonance-electrical-impedance-tomography
#15
Matej Kranjc, Simona Kranjc, Franci Bajd, Gregor Serša, Igor Serša, Damijan Miklavčič
Irreversible electroporation (IRE) is gaining importance in routine clinical practice for nonthermal ablation of solid tumors. For its success, it is extremely important that the coverage and exposure time of the treated tumor to the electric field is within the specified range. Measurement of electric field distribution during the electroporation treatment can be achieved using magnetic resonance electrical impedance tomography (MREIT). Here, we show improved MREIT-enabled electroporation monitoring of IRE-treated tumors by predicting IRE-ablated tumor areas during IRE of mouse tumors in vivo...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28864693/ablation-with-irreversible-electroporation-in-patients-with-advanced-perihilar-cholangiocarcinoma-alpaca-a-multicentre-phase-i-ii-feasibility-study-protocol
#16
Robert J S Coelen, Jantien A Vogel, Laurien G P H Vroomen, Eva Roos, Olivier R C Busch, Otto M van Delden, Foke van Delft, Michal Heger, Jeanin E van Hooft, Geert Kazemier, Heinz-Josef Klümpen, Krijn P van Lienden, Erik A J Rauws, Hester J Scheffer, Henk M Verheul, Jan de Vries, Johanna W Wilmink, Barbara M Zonderhuis, Marc G Besselink, Thomas M van Gulik, Martijn R Meijerink
INTRODUCTION: The majority of patients with perihilar cholangiocarcinoma (PHC) has locally advanced disease or distant lymph node metastases on presentation or exploratory laparotomy, which makes them not eligible for resection. As the prognosis of patients with locally advanced PHC or lymph node metastases in the palliative setting is significantly better compared with patients with organ metastases, ablative therapies may be beneficial. Unfortunately, current ablative options are limited...
September 1, 2017: BMJ Open
https://www.readbyqxmd.com/read/28860595/intestinal-stem-cells-contribute-to-the-maturation-of-the-neonatal-small-intestine-and-colon-independently-of-digestive-activity
#17
Hirotsugu Yanai, Naho Atsumi, Toshihiro Tanaka, Naohiro Nakamura, Yoshihiro Komai, Taichi Omachi, Kiyomichi Tanaka, Kazuhiko Ishigaki, Kazuho Saiga, Haruyuki Ohsugi, Yoko Tokuyama, Yuki Imahashi, Shuichi Ohe, Hiroko Hisha, Naoko Yoshida, Keiki Kumano, Masanori Kon, Hiroo Ueno
The murine intestine, like that of other mammalians, continues to develop after birth until weaning; however, whether this occurs in response to an intrinsic developmental program or food intake remains unclear. Here, we report a novel system for the allotransplantation of small intestine and colon harvested from Lgr5 (EGFP-IRES-CreERT2/+); Rosa26 (rbw/+) mice immediately after birth into the subrenal capsule of wild-type mice. By histological and immunohistochemical analysis, the developmental process of transplanted small intestine and colon was shown to be comparable with that of the native tissues: mature intestines equipped with all cell types were formed, indicating that these organs do not require food intake for development...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28854224/the-5-poly-a-leader-of-poxvirus-mrna-confers-a-translational-advantage-that-can-be-achieved-in-cells-with-impaired-cap-dependent-translation
#18
Pragyesh Dhungel, Shuai Cao, Zhilong Yang
The poly(A) leader at the 5'-untranslated region (5'-UTR) is an unusually striking feature of all poxvirus mRNAs transcribed after viral DNA replication (post-replicative mRNAs). These poly(A) leaders are non-templated and of heterogeneous lengths; and their function during poxvirus infection remains a long-standing question. Here, we discovered that a 5'-poly(A) leader conferred a selective translational advantage to mRNA in poxvirus-infected cells. A constitutive and uninterrupted 5'-poly(A) leader with 12 residues was optimal...
August 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28843546/ljungan-sebokele-like-picornavirus-in-birds-of-prey-common-kestrel-falco-tinnunculus-and-red-footed-falcon-f-vespertinus
#19
Péter Pankovics, Ákos Boros, Róbert Mátics, Beatrix Kapusinszky, Eric Delwart, Gábor Reuter
Ljungan and Sebokele viruses are thought to be rodent-borne (picorna)viruses in the genus Parechovirus. Using random amplification and next generation sequencing method a novel Ljungan/Sebokele-like picornavirus was identified in birds of prey. Viral RNA was detected in total of 1 (9%) of the 11 and 2 (28.6%) of the 7 faecal samples from common kestrels and red-footed falcons in Hungary, respectively. High faecal viral RNA load (4.77×10(6) genomic copies/ml) measured by qPCR. The complete genome of picornavirus strain falcon/HA18_080/2014/HUN (KY645497) is 7964-nucleotide (nt) long including a 867-nt 5'end and a 101-nt 3'end (excluding the poly(A)-tail)...
August 24, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28840060/mitochondrial-ferritin-protects-hydrogen-peroxide-induced-neuronal-cell-damage
#20
Guofen Gao, Nan Zhang, Yue-Qi Wang, Qiong Wu, Peng Yu, Zhen-Hua Shi, Xiang-Lin Duan, Bao-Lu Zhao, Wen-Shuang Wu, Yan-Zhong Chang
Oxidative stress and iron accumulation are tightly associated with neurodegenerative diseases. Mitochondrial ferritin (FtMt) is identified as an iron-storage protein located in the mitochondria, and its role in regulation of iron hemeostasis in neurodegenerative diseases has been reported. However, the role of FtMt in hydrogen peroxide (H2O2)-induced oxidative stress and iron accumulation in neuronal cells has not been studied. Here, we overexpressed FtMt in neuroblastoma SH-SY5Y cells and induced oxidative stress by treating with extracellular H2O2...
July 2017: Aging and Disease
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