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https://www.readbyqxmd.com/read/28081156/generation-of-a-stable-transgenic-swine-model-expressing-a-porcine-histone-2b-egfp-fusion-protein-for-cell-tracking-and-chromosome-dynamics-studies
#1
Renan B Sper, Sehwon Koh, Xia Zhang, Sean Simpson, Bruce Collins, Jeff Sommer, Robert M Petters, Ignacio Caballero, Jeff L Platt, Jorge A Piedrahita
Transgenic pigs have become an attractive research model in the field of translational research, regenerative medicine, and stem cell therapy due to their anatomic, genetic and physiological similarities with humans. The development of fluorescent proteins as molecular tags has allowed investigators to track cell migration and engraftment levels after transplantation. Here we describe the development of two transgenic pig models via SCNT expressing a fusion protein composed of eGFP and porcine Histone 2B (pH2B)...
2017: PloS One
https://www.readbyqxmd.com/read/28080113/higher-order-cognitive-control-in-dual-tasks-evidence-from-task-pair-switching
#2
Patricia Hirsch, Sophie Nolden, Iring Koch
In the present study, we combined the psychological refractory period (PRP) paradigm with a novel task-pair switching logic which enabled us to isolate performance costs occurring at the global level of task-pairs. In Experiment 1, in which we used conceptually overlapping responses for Task 1 (T1) and Task 2 (T2), we generated 3 task-pairs by combining 1 of 3 visual tasks (T1) with an auditory task (T2). In addition to worse performance after a short SOA than a long SOA (i.e., PRP effect), we found impaired performance in n - 1 task-pair switches as compared to n - 1 task-pair repetitions (i...
January 12, 2017: Journal of Experimental Psychology. Human Perception and Performance
https://www.readbyqxmd.com/read/28078482/irreversible-electroporation-as-treatment-of-locally-advanced-and-as-margin-accentuation-in-borderline-resectable-pancreatic-adenocarcinoma
#3
P Marsanic, A Mellano, A Sottile, M De Simone
In recent years, many local ablation technologies based on thermal damage have been used in the treatment of locally advanced pancreatic carcinoma (LAPC) and borderline resectable pancreatic carcinoma (BLRPC). However, they are associated with major complications because of possible vascular and ductal damage. Irreversible electroporation (IRE) is a nonthermal ablation technology that seems safe near vital vascular and ductal structures. IRE could be used as exclusive treatment of LAPC (en situ to IRE) after induction chemotherapy In BLRPC, surgery is not really radical in 6% of patients (microscopic residual) and local recurrences occur in 11-42% of apparent radical resections...
January 11, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28078320/lgr4-and-lgr5-function-redundantly-during-human-endoderm-differentiation
#4
Yu-Hwai Tsai, David R Hill, Namit Kumar, Sha Huang, Alana M Chin, Briana R Dye, Melinda S Nagy, Michael P Verzi, Jason R Spence
BACKGROUND & AIMS: The Lgr family of transmembrane proteins (Lgr4, 5, 6) act as functional receptors for R-spondin proteins (Rspo 1, 2, 3, 4), and potentiate Wnt signaling in different contexts. Lgr5 is arguably the best characterized of the Lgr family members in a number of adult and embryonic contexts in mice. However, the function of LGR family members in early embryonic development is unclear, and has not been explored during human development or tissue differentiation in detail. METHODS: We interrogated the function and expression of LGR family members using human pluripotent stem cell-derived tissues including definitive endoderm, mid/hindgut, and intestinal organoids...
September 2016: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28077561/micrornas-stimulate-translation-initiation-mediated-by-hcv-like-ireses
#5
Chloé Mengardi, Taran Limousin, Emiliano P Ricci, Ricardo Soto-Rifo, Didier Decimo, Théophile Ohlmann
MicroRNAs (miRNAs) are small non-coding RNAs that control gene expression by recognizing and hybridizing to a specific sequence generally located in the 3' untranslated region (UTR) of targeted mRNAs. miRNA-induced inhibition of translation occurs during the initiation step, most probably at the level of ribosome scanning. In this process, the RNA-induced silencing complex interacts both with PABP and the 43S pre-initiation complex to disrupt scanning of the 40S ribosome. However, in some specific cases, miRNAs can stimulate translation...
January 10, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28067773/a-systems-biological-view-of-life-and-death-decision-with-respect-to-endoplasmic-reticulum-stress-the-role-of-perk-pathway
#6
Margita Márton, Anita Kurucz, Beáta Lizák, Éva Margittai, Gábor Bánhegyi, Orsolya Kapuy
Accumulation of misfolded/unfolded proteins in the endoplasmic reticulum (ER) leads to the activation of three branches (Protein kinase (RNA)-like endoplasmic reticulum kinase [PERK], Inositol requiring protein 1 [IRE-1] and Activating trascription factor 6 [ATF6], respectively) of unfolded protein response (UPR). The primary role of UPR is to try to drive back the system to the former or a new homeostatic state by self-eating dependent autophagy, while excessive level of ER stress results in apoptotic cell death...
January 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28056049/asc-induces-apoptosis-via-activation-of-caspase-9-by-enhancing-gap-junction-mediated-intercellular-communication
#7
Masato Kitazawa, Shigeaki Hida, Chifumi Fujii, Shun'ichiro Taniguchi, Kensuke Ito, Tomio Matsumura, Nagisa Okada, Takashi Sakaizawa, Akira Kobayashi, Michiko Takeoka, Shin-Ichi Miyagawa
ASC (apoptosis-associated speck-like protein containing a CARD) is a key adaptor molecule of inflammasomes that mediates inflammatory and apoptotic signals. Aberrant methylation-induced silencing of ASC has been observed in a variety of cancer cells, thus implicating ASC in tumor suppression, although this role remains incompletely defined especially in the context of closely neighboring cell proliferation. As ASC has been confirmed to be silenced by abnormal methylation in HT1080 fibrosarcoma cells as well, this cell line was investigated to characterize the precise role and mechanism of ASC in tumor progression...
2017: PloS One
https://www.readbyqxmd.com/read/28054974/targeting-ires-mediated-p53-synthesis-for-cancer-diagnosis-and-therapeutics
#8
REVIEW
Bai Ji, Benjamin R E Harris, Yahui Liu, Yibin Deng, Sergio A Gradilone, Margot P Cleary, Jianhua Liu, Da-Qing Yang
While translational regulation of p53 by the internal ribosome entry site (IRES) at its 5'-untranslated region following DNA damage has been widely accepted, the detailed mechanism underlying the translational control of p53 by its IRES sequence is still poorly understood. In this review, we will focus on the latest progress in identifying novel regulatory proteins of the p53 IRES and in uncovering the functional connection between defective IRES-mediated p53 translation and tumorigenesis. We will also discuss how these findings may lead to a better understanding of the process of oncogenesis and open up new avenues for cancer diagnosis and therapeutics...
January 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28052375/unexplained-isolated-hyperferritinemia-without-iron-overload
#9
Giulia Ravasi, Sara Pelucchi, Raffaella Mariani, Marco Casati, Federico Greni, Cristina Arosio, Irene Pelloni, Silvia Majore, Paolo Santambrogio, Sonia Levi, Alberto Piperno
Although hyperferritinemia may be reflective of elevated total body iron stores, there are conditions in which ferritin levels are disproportionately elevated relative to iron status. Autosomal dominant forms of hyperferritinemia due to mutations in the L-ferritin IRE or in A helix of L-ferritin gene have been described, however cases of isolated hyperferritinemia still remain unsolved. We describe 12 Italian subjects with unexplained isolated hyperferritinemia (UIH). Four probands have affected siblings, but no affected parents or offspring...
January 4, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28052102/irreversible-electroporation-of-the-pancreas-using-parallel-plate-electrodes-in-a-porcine-model-a-feasibility-study
#10
Steffi J E Rombouts, Maarten W Nijkamp, Willemijn P M van Dijck, Lodewijk A A Brosens, Maurits Konings, R van Hillegersberg, Inne H M Borel Rinkes, Jeroen Hagendoorn, Fred H Wittkampf, I Quintus Molenaar
BACKGROUND: Irreversible electroporation (IRE) with needle electrodes is being explored as treatment option in locally advanced pancreatic cancer. Several studies have shown promising results with IRE needles, positioned around the tumor to achieve tumor ablation. Disadvantages are the technical difficulties for needle placement, the time needed to achieve tumor ablation, the risk of needle track seeding and most important the possible occurrence of postoperative pancreatic fistula via the needle tracks...
2017: PloS One
https://www.readbyqxmd.com/read/28046134/the-triticum-mosaic-virus-5-leader-binds-to-both-eif4g-and-eifiso4g-for-translation
#11
Robyn Roberts, Laura K Mayberry, Karen S Browning, Aurélie M Rakotondrafara
We recently identified a remarkably strong (739 nt-long) IRES-like element in the 5' untranslated region (UTR) of Triticum mosaic virus (TriMV, Potyviridae). Here, we define the components of the cap-binding translation initiation complex that are required for TriMV translation. Using bio-layer interferometry and affinity capture of the native translation apparatus, we reveal that the viral translation element has a ten-fold greater affinity for the large subunit eIF4G/eIFiso4G than to the cap binding protein eIF4E/eIFiso4E...
2017: PloS One
https://www.readbyqxmd.com/read/28038349/imine-reductases-ireds
#12
REVIEW
Juan Mangas-Sanchez, Scott P France, Sarah L Montgomery, Godwin A Aleku, Henry Man, Mahima Sharma, Jeremy I Ramsden, Gideon Grogan, Nicholas J Turner
Imine reductases (IREDs) have emerged as a valuable new set of biocatalysts for the asymmetric synthesis of optically active amines. The development of bioinformatics tools and searchable databases has led to the identification of a diverse range of new IRED biocatalysts that have been characterised and employed in different synthetic processes. This review describes the latest developments in the structural and mechanistic aspects of IREDs, together with synthetic applications of these enzymes, and identifies ongoing and future challenges in the field...
December 27, 2016: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28032634/k%C3%A3-lliker-fuse-gabaergic-and-glutamatergic-neurons-project-to-distinct-targets
#13
Joel Geerling, Shigefumi Yokota, Irma Rukhadze, Dan Roe, Nancy L Chamberlin
The Kölliker-Fuse nucleus (KF) is known primarily for its respiratory function as the "pneumotaxic center" or "pontine respiratory group." Considered part of the parabrachial (PB) complex, KF contains glutamatergic neurons that project to respiratory-related targets in the medulla and spinal cord (Yokota et al., 2007). Here we describe an unexpected population of neurons in the caudal KF and adjacent lateral crescent subnucleus (PBlc), which are GABAergic and have an entirely different pattern of projections than glutamatergic KF neurons...
December 29, 2016: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28031527/tumor-initiating-cells-of-breast-and-prostate-origin-show-alterations-in-the-expression-of-genes-related-to-iron-metabolism
#14
Zuzana Rychtarcikova, Sandra Lettlova, Veronika Tomkova, Vlasta Korenkova, Lucie Langerova, Ekaterina Simonova, Polina Zjablovskaja, Meritxell Alberich-Jorda, Jiri Neuzil, Jaroslav Truksa
The importance of iron in the growth and progression of tumors has been widely documented. In this report, we show that tumor-initiating cells (TICs), represented by spheres derived from the MCF7 cell line, exhibit higher intracellular labile iron pool, mitochondrial iron accumulation and are more susceptible to iron chelation. TICs also show activation of the IRP/IRE system, leading to higher iron uptake and decrease in iron storage, suggesting that level of properly assembled cytosolic iron-sulfur clusters (FeS) is reduced...
December 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/28017505/endurance-training-induced-changes-in-the-gh-igf-i-axis-influence-maximal-muscle-strength-in-previously-untrained-men
#15
Marcin Grandys, Joanna Majerczak, Piotr Kuczek, Krystyna Sztefko, Krzysztof Duda, Jerzy A Zoladz
OBJECTIVE: In this study we have determined the effects of 20weeks of endurance running training on the GH-IGF-I axis changes in the context of the skeletal muscle performance and physical capacity level. DESIGN: Before and after the endurance training program a maximal incremental exercise tests, a 1500m race and a muscle strength measurements were performed and the blood samples were taken to determine both resting as well as end-exercise serum growth hormone (GH), insulin-like growth hormone-I (IGF-I), insulin-like growth hormone binding protein-3 (IGFBP-3) and plasma interleukin-6 (IL-6) concentrations...
December 15, 2016: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/28012572/mammalian-polycistronic-mrnas-and-disease
#16
REVIEW
Timofey A Karginov, Daniel Parviz Hejazi Pastor, Bert L Semler, Christopher M Gomez
Our understanding of gene expression has come far since the 'one-gene one-polypeptide' hypothesis proposed by Beadle and Tatum. In this review, we address the gradual recognition that a growing number of polycistronic genes, originally discovered in viruses, are being identified within the mammalian genome, and that these may provide new insights into disease mechanisms and treatment. We carried out a systematic literature review identifying 13 mammalian genes for which there is evidence for polycistronic expression via translation through an internal ribosome entry site (IRES)...
December 21, 2016: Trends in Genetics: TIG
https://www.readbyqxmd.com/read/28009979/ablation-of-hepatic-malignant-tumors-with-irreversible-electroporation-a-systematic-review-and-meta-analysis-of-outcomes
#17
Guo Tian, Qiyu Zhao, Fen Chen, Tian'an Jiang, Weilin Wang
BACKGROUND: Irreversible electroporation (IRE) ablation is a new technique that is used to eliminate malignant tumors through nonthermal approaches. OBJECTIVE: The purpose of this review was to evaluate the efficiency of IRE for hepatic malignant tumors. METHODS: A systematic search was performed from PubMed, Embase, Web of science, Scopus and other potential literatures from references in relevant articles July 26th, 2016. Overall estimates of pooled standard mean difference (SMD) with 95% confidence interval (CI) were calculated for the changes of the pre- and post-IRE longest diameter, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and serum total bilirubin levels...
December 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/28009256/translation-initiation-by-the-hepatitis-c-virus-ires-requires-eif1a-and-ribosomal-complex-remodeling
#18
Zane A Jaafar, Akihiro Oguro, Yoshikazu Nakamura, Jeffrey S Kieft
Internal ribosome entry sites (IRESs) are important RNA-based translation initiation signals, critical for infection by many pathogenic viruses. The hepatitis C virus (HCV) IRES is the prototype for the type 3 IRESs and is also invaluable for exploring principles of eukaryotic translation initiation, in general. Current mechanistic models for the type 3 IRESs are useful but they also present paradoxes, including how they can function both with and without eukaryotic initiation factor (eIF) 2. We discovered that eIF1A is necessary for efficient activity where it stabilizes tRNA binding and inspects the codon-anticodon interaction, especially important in the IRES' eIF2-independent mode...
December 23, 2016: ELife
https://www.readbyqxmd.com/read/28005212/genetic-characterization-of-a-second-novel-picornavirus-from-an-amphibian-host-smooth-newt-lissotriton-vulgaris
#19
Péter Pankovics, Ákos Boros, Zoltán Tóth, Tung Gia Phan, Eric Delwart, Gábor Reuter
In this study, a novel picornavirus was identified in faecal samples from smooth newts (Lissotriton vulgaris). The complete genome of picornavirus strain newt/II-5-Pilis/2014/HUN (KX463670) is 7755 nt long with type-IV IRES and has 39.6% aa sequence identity in the protein P1 to the corresponding protein of bat picornavirus (KJ641686, unassigned) and 42.7% and 53.5% aa sequence identity in the 2C and 3CD protein, respectively, to oscivirus (GU182410, genus Oscivirus). Interestingly, the L-protein of newt/II-5-Pilis/2014/HUN has conserved aa motifs that are similar to those found in phosphatase-1 catalytic (PP1C) subunit binding region (pfam10488) proteins...
December 22, 2016: Archives of Virology
https://www.readbyqxmd.com/read/28000152/inactivation-of-bone-morphogenetic-protein-1-bmp1-and-tolloid-like-1-tll1-in-cells-expressing-type-i-collagen-leads-to-dental-and-periodontal-defects-in-mice
#20
Hua Zhang, Priyam Jani, Tian Liang, Yongbo Lu, Chunlin Qin
Bone morphogenetic protein 1 (BMP1) and tolloid-like 1 (TLL1) belong to the BMP1/tolloid-like proteinase family, which cleaves secretory proteins. The constitutive deletion of the Bmp1 or Tll1 genes causes perinatal or embryonic lethality in mice. In this study, we first studied the β-galactosidase activity in mice in which an IRES-lacZ-Neo cassette was inserted in the intron of either the Bmp1 or the Tll1 gene; the β-galactosidase activities were used to reflect the expression of endogenous Bmp1 and Tll1, respectively...
December 20, 2016: Journal of Molecular Histology
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