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https://www.readbyqxmd.com/read/29349506/new-perspectives-on-human-multitasking
#1
EDITORIAL
Edita Poljac, Andrea Kiesel, Iring Koch, Hermann Müller
No abstract text is available yet for this article.
January 18, 2018: Psychological Research
https://www.readbyqxmd.com/read/29343848/a-novel-protein-encoded-by-the-circular-form-of-the-shprh-gene-suppresses-glioma-tumorigenesis
#2
Maolei Zhang, Nunu Huang, Xuesong Yang, Jingyan Luo, Sheng Yan, Feizhe Xiao, Wenping Chen, Xinya Gao, Kun Zhao, Huangkai Zhou, Ziqiang Li, Liu Ming, Bo Xie, Nu Zhang
Circular RNAs (circRNAs) are recognized as functional non-coding transcripts in eukaryotic cells. Recent evidence has indicated that even though circRNAs are generally expressed at low levels, they may be involved in many physiological or pathological processes, such as gene regulation, tissue development and carcinogenesis. Although the 'microRNA sponge' function is well characterized, most circRNAs do not contain perfect trapping sites for microRNAs, which suggests the possibility that circRNAs have functions that have not yet been defined...
January 18, 2018: Oncogene
https://www.readbyqxmd.com/read/29343741/species-distribution-modelling-contrasting-presence-only-models-with-plot-abundance-data
#3
Vitor H F Gomes, Stéphanie D IJff, Niels Raes, Iêda Leão Amaral, Rafael P Salomão, Luiz de Souza Coelho, Francisca Dionízia de Almeida Matos, Carolina V Castilho, Diogenes de Andrade Lima Filho, Dairon Cárdenas López, Juan Ernesto Guevara, William E Magnusson, Oliver L Phillips, Florian Wittmann, Marcelo de Jesus Veiga Carim, Maria Pires Martins, Mariana Victória Irume, Daniel Sabatier, Jean-François Molino, Olaf S Bánki, José Renan da Silva Guimarães, Nigel C A Pitman, Maria Teresa Fernandez Piedade, Abel Monteagudo Mendoza, Bruno Garcia Luize, Eduardo Martins Venticinque, Evlyn Márcia Moraes de Leão Novo, Percy Núñez Vargas, Thiago Sanna Freire Silva, Angelo Gilberto Manzatto, John Terborgh, Neidiane Farias Costa Reis, Juan Carlos Montero, Katia Regina Casula, Beatriz S Marimon, Ben-Hur Marimon, Euridice N Honorio Coronado, Ted R Feldpausch, Alvaro Duque, Charles Eugene Zartman, Nicolás Castaño Arboleda, Timothy J Killeen, Bonifacio Mostacedo, Rodolfo Vasquez, Jochen Schöngart, Rafael L Assis, Marcelo Brilhante Medeiros, Marcelo Fragomeni Simon, Ana Andrade, William F Laurance, José Luís Camargo, Layon O Demarchi, Susan G W Laurance, Emanuelle de Sousa Farias, Henrique Eduardo Mendonça Nascimento, Juan David Cardenas Revilla, Adriano Quaresma, Flavia R C Costa, Ima Célia Guimarães Vieira, Bruno Barçante Ladvocat Cintra, Hernán Castellanos, Roel Brienen, Pablo R Stevenson, Yuri Feitosa, Joost F Duivenvoorden, Gerardo A Aymard C, Hugo F Mogollón, Natalia Targhetta, James A Comiskey, Alberto Vicentini, Aline Lopes, Gabriel Damasco, Nállarett Dávila, Roosevelt García-Villacorta, Carolina Levis, Juliana Schietti, Priscila Souza, Thaise Emilio, Alfonso Alonso, David Neill, Francisco Dallmeier, Leandro Valle Ferreira, Alejandro Araujo-Murakami, Daniel Praia, Dário Dantas do Amaral, Fernanda Antunes Carvalho, Fernanda Coelho de Souza, Kenneth Feeley, Luzmila Arroyo, Marcelo Petratti Pansonato, Rogerio Gribel, Boris Villa, Juan Carlos Licona, Paul V A Fine, Carlos Cerón, Chris Baraloto, Eliana M Jimenez, Juliana Stropp, Julien Engel, Marcos Silveira, Maria Cristina Peñuela Mora, Pascal Petronelli, Paul Maas, Raquel Thomas-Caesar, Terry W Henkel, Doug Daly, Marcos Ríos Paredes, Tim R Baker, Alfredo Fuentes, Carlos A Peres, Jerome Chave, Jose Luis Marcelo Pena, Kyle G Dexter, Miles R Silman, Peter Møller Jørgensen, Toby Pennington, Anthony Di Fiore, Fernando Cornejo Valverde, Juan Fernando Phillips, Gonzalo Rivas-Torres, Patricio von Hildebrand, Tinde R van Andel, Ademir R Ruschel, Adriana Prieto, Agustín Rudas, Bruce Hoffman, César I A Vela, Edelcilio Marques Barbosa, Egleé L Zent, George Pepe Gallardo Gonzales, Hilda Paulette Dávila Doza, Ires Paula de Andrade Miranda, Jean-Louis Guillaumet, Linder Felipe Mozombite Pinto, Luiz Carlos de Matos Bonates, Natalino Silva, Ricardo Zárate Gómez, Stanford Zent, Therany Gonzales, Vincent A Vos, Yadvinder Malhi, Alexandre A Oliveira, Angela Cano, Bianca Weiss Albuquerque, Corine Vriesendorp, Diego Felipe Correa, Emilio Vilanova Torre, Geertje van der Heijden, Hirma Ramirez-Angulo, José Ferreira Ramos, Kenneth R Young, Maira Rocha, Marcelo Trindade Nascimento, Maria Natalia Umaña Medina, Milton Tirado, Ophelia Wang, Rodrigo Sierra, Armando Torres-Lezama, Casimiro Mendoza, Cid Ferreira, Cláudia Baider, Daniel Villarroel, Henrik Balslev, Italo Mesones, Ligia Estela Urrego Giraldo, Luisa Fernanda Casas, Manuel Augusto Ahuite Reategui, Reynaldo Linares-Palomino, Roderick Zagt, Sasha Cárdenas, William Farfan-Rios, Adeilza Felipe Sampaio, Daniela Pauletto, Elvis H Valderrama Sandoval, Freddy Ramirez Arevalo, Isau Huamantupa-Chuquimaco, Karina Garcia-Cabrera, Lionel Hernandez, Luis Valenzuela Gamarra, Miguel N Alexiades, Susamar Pansini, Walter Palacios Cuenca, William Milliken, Joana Ricardo, Gabriela Lopez-Gonzalez, Edwin Pos, Hans Ter Steege
Species distribution models (SDMs) are widely used in ecology and conservation. Presence-only SDMs such as MaxEnt frequently use natural history collections (NHCs) as occurrence data, given their huge numbers and accessibility. NHCs are often spatially biased which may generate inaccuracies in SDMs. Here, we test how the distribution of NHCs and MaxEnt predictions relates to a spatial abundance model, based on a large plot dataset for Amazonian tree species, using inverse distance weighting (IDW). We also propose a new pipeline to deal with inconsistencies in NHCs and to limit the area of occupancy of the species...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343570/roles-of-the-5-untranslated-region-of-non-primate-hepacivirus-in-translation-initiation-and-viral-replication
#4
Tomohisa Tanaka, Teruhime Otoguro, Atsuya Yamashita, Hirotake Kasai, Takasuke Fukuhara, Yoshiharu Matsuura, Kohji Moriishi
The 5' untranslated region (UTR) of hepatitis C virus (HCV), which is composed of four domains (I, II, III and IV) and a pseudoknot, is essential for translation and viral replication. Equine non-primate hepacivirus (EHcV) harbors a 5' UTR consisting of a large 5' -terminal domain (I), three additional domains (I', II and III), which are homologous to domains I, II and III, respectively, of HCV, and a pseudoknot, in the order listed. In this study, we investigated the roles of the EHcV 5' UTR in translation and viral replication...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29343429/translational-control-of-human-acetyl-coa-carboxylase-1-mrna-is-mediated-by-an-internal-ribosome-entry-site-in-response-to-er-stress-serum-deprivation-or-hypoxia-mimetic-cocl2
#5
Fabrizio Damiano, Mariangela Testini, Romina Tocci, Gabriele V Gnoni, Luisa Siculella
Acetyl-CoA carboxylase 1 (ACC1) is a cytosolic enzyme catalyzing the rate limiting step in de novo fatty acid biosynthesis. There is mounting evidence showing that ACC1 is susceptible to dysregulation and that it is over-expressed in liver diseases associated with lipid accumulation and in several cancers. In the present study, ACC1 regulation at the translational level is reported. Using several experimental approaches, the presence of an internal ribosome entry site (IRES) has been established in the 5' untranslated region (5' UTR) of the ACC1 mRNA...
January 14, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29342112/useful-bicistronic-reporter-system-for-studying-poly-a-site-defining-cis-elements-and-regulation-of-alternative-polyadenylation
#6
Zhongyuan Deng, Shen Zhang, Shaohua Gu, Xinzhi Ni, Wenxian Zeng, Xianchun Li
The link between polyadenylation (pA) and various biological, behavioral, and pathological events of eukaryotes underlines the need to develop in vivo polyadenylation assay methods for characterization of the cis-acting elements, trans-acting factors and environmental stimuli that affect polyadenylation efficiency and/or relative usage of two alternative polyadenylation (APA) sites. The current protein-based CAT or luciferase reporter systems can measure the polyadenylation efficiency of a single pA site or candidate cis element but not the choice of two APA sites...
January 17, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29337371/avian-retrovirus-mediated-tumor-specific-gene-knockout
#7
Wei Wang, Bingning Dong, Feng Yang
The RCAS (replication-competent avian sarcoma leukosis virus long-terminal repeat with splice acceptor)-TVA (tumor virus A) gene delivery system has been successfully used in modeling human cancers. Based on this, we have recently developed a novel RCI-Oncogene (RCAS-Cre-IRES-Oncogene) gene delivery system that can be used to efficiently manipulate gene expression in spontaneous tumors in vivo. We used this system for tumor gene knockout (TuKO) and demonstrated a crucial role of FGFR1 in driving mammary tumor metastasis...
January 16, 2018: Current Protocols in Molecular Biology
https://www.readbyqxmd.com/read/29337050/impairments-in-social-novelty-recognition-and-spatial-memory-in-mice-with-conditional-deletion-of-scn1a-in-parvalbumin-expressing-cells
#8
Tetsuya Tatsukawa, Ikuo Ogiwara, Emi Mazaki, Atsushi Shimohata, Kazuhiro Yamakawa
Loss of function mutations in the SCN1A gene, which encodes the voltage-gated sodium channel Nav1.1, have been described in the majority of Dravet syndrome patients presenting with epileptic seizures, hyperactivity, autistic traits, and cognitive decline. We previously reported predominant Nav1.1 expression in parvalbumin-expressing (PV+) inhibitory neurons in juvenile mouse brain and observed epileptic seizures in mice with selective deletion of Scn1a in PV+ cells mediated by PV-Cre transgene expression (Scn1afl/+/PV-Cre-TG)...
January 11, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29335228/irreversible-electroporation-for-locally-advanced-pancreatic-cancer-through-a-minimally-invasive-surgery-supported-by-laparoscopic-ultrasound
#9
Ernesto Tartaglia, Massimiliano Fabozzi, Antonia Rizzuto, Anna Settembre, Roberta Abete, Ludovica Guerriero, Pasqualino Favoriti, Diego Cuccurullo, Francesco Corcione
INTRODUCTION: Pancreatic cancer is one of the most lethal cancers worldwide, with 5-years survival rate as low as 6%. The majority of pancreatic cancer patients present locally advanced or metastatic disease at diagnosis. Typically, patients affected by locally advanced pancreatic cancer (LAPC) do not undergo radical surgery but are treated with focal ablative therapies. However, a high rate of morbidity due to the heat sink effect has limited the application of ablative techniques on a routine basis in LAPC patients...
December 28, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29333120/focused-transhepatic-electroporation-mediated-by-hypersaline-infusion-through-the-portal-vein-in-rat-model-preliminary-results-on-differential-conductivity
#10
Clara Pañella, Quim Castellví, Xavier Moll, Rita Quesada, Alberto Villanueva, Mar Iglesias, Dolores Naranjo, Patricia Sánchez-Velázquez, Anna Andaluz, Luís Grande, Antoni Ivorra, Fernando Burdío
Background: Spread hepatic tumours are not suitable for treatment either by surgery or conventional ablation methods. The aim of this study was to evaluate feasibility and safety of selectively increasing the healthy hepatic conductivity by the hypersaline infusion (HI) through the portal vein. We hypothesize this will allow simultaneous safe treatment of all nodules by irreversible electroporation (IRE) when applied in a transhepatic fashion. Material and methods: Sprague Dawley (Group A, n = 10) and Athymic rats with implanted hepatic tumour (Group B, n = 8) were employed...
December 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/29330752/evaluation-of-the-iron-regulatory-protein-1-interactome
#11
Gregory J Connell, Jando S Danial, Christian X Haastruthers
The interactions of iron regulatory proteins (IRPs) with mRNAs containing an iron-responsive element (IRE) is a major means through which intracellular iron homeostasis is maintained and integrated with cellular function. Although IRE-IRP interactions have been proposed to modulate the expression of a diverse number of mRNAs, a transcriptome analysis of the interactions that form within the native mRNA structure and cellular environment has not previously been described. An RNA-CLIP study is described here that identified IRP-1 interactions occurring within a primary cell line expressing physiologically relevant amounts of mRNA and protein...
January 12, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29327233/dcaf1-is-involved-in-hcv-replication-through-regulation-of-mir-122
#12
Yanling Yan, Conghui Li, Binlian Sun, Rongge Yang
Hepatitis C virus (HCV) is a worldwide threaten to human health with a high ratio of chronic infections. Recently, we found that Vpr-mediated regulation of HCV replication depends on the host protein DDB1-Cul4 associate factor 1 (DCAF1), implying that DCAF1 might be involved in the replication of HCV. In this study, we demonstrated that DCAF1 knockdown reduced HCV replication both in the infectious (JFH1) and replicon (Con1) systems. Further investigation showed a negative regulation of HCV internal ribosome entry site (IRES)-mediated translation by DCAF1...
January 11, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29322444/constructing-gfp-based-reporter-to-study-back-splicing-and-translation-of-circular-rna
#13
Yun Yang, Zefeng Wang
Human transcriptome contains a large number of circular RNAs (circRNAs) that are mainly produced by back splicing of pre-mRNA. Here we describe a minigene reporter system containing a single exon encoding split GFP in reverse order, which can be efficiently back spliced to produce a circRNA encoding intact GFP gene. This simple reporter system can be adopted to study how different cis-elements and trans-factors affect circRNA production, and also can serve as a reliable system to measure the activity of IRES-mediated translation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29322302/the-superiority-of-sucrose-cushion-centrifugation-to-ultrafiltration-and-pegylation-in-generating-high-titer-lentivirus-particles-and-transducing-stem-cells-with-enhanced-efficiency
#14
Mahdi Eskandarian Boroujeni, Mossa Gardaneh
Viral gene delivery is hailed as a great milestone in gene-based therapeutic approaches. The human immunodeficiency virus-derived lentiviral vectors (LVs) are advantageous in infecting both dividing and non-dividing cells leading to continuous expression of transgenes. A variety of protocols are available for concentration of LVs. We primarily generated our internal ribosome entry site (IRES)-based LVs. Virus titration and transduction efficiency were compared between various strategies that included sucrose cushion centrifugation (SCC), protein column ultrafiltration and polyethylene glycol precipitation...
January 10, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29320583/a-multi-center-open-label-prospective-study-of-cannula-injection-of-small-particle-hyaluronic-acid-plus-lidocaine-sphal-for-lip-augmentation
#15
Raj Chopra, Miles Graivier, Sabrina Fabi, Mark Nestor, Patricia Meuse, Jay Mashburn
<p>BACKGROUND: The use of blunt-tipped microcannulas for injection of hyaluronic acid (HA) filler in the lip and perioral area has gained popularity as they provide important safety-related advantages compared to traditional hypodermic needles. This study was conducted to assess the safety and effectiveness associated with the use of a blunt-tipped microcannula for lip augmentation and correction of perioral rhytids using a small-particle, hyaluronic acid gel plus lidocaine (SPHAL).</p> <p>METHODS: A multi-center, open-label, prospective, study enrolled 60 subjects...
January 1, 2018: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29317744/a-role-for-divalent-metal-transporter-dmt1-in-mitochondrial-uptake-of-iron-and-manganese
#16
Natascha A Wolff, Michael D Garrick, Lin Zhao, Laura M Garrick, Andrew J Ghio, Frank Thévenod
Much of iron and manganese metabolism occurs in mitochondria. Uptake of redox-active iron must be tightly controlled, but little is known about how metal ions enter mitochondria. Recently, we established that the divalent metal transporter 1 (DMT1) is present in the outer mitochondrial membrane (OMM). Therefore we asked if it mediates Fe2+ and Mn2+ influx. Mitochondria were isolated from HEK293 cells permanently transfected with inducible rat DMT1 isoform 1 A/+IRE (HEK293-rDMT1). Fe2+-induced quenching of the dye PhenGreen™SK (PGSK) occurred in two phases, one of which reflected OMM DMT1 with stronger Fe2+ uptake after DMT1 overexpression...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29316982/the-tankyrase-inhibitor-g007-lk-inhibits-small-intestine-lgr5-stem-cell-proliferation-without-altering-tissue-morphology
#17
Jens Henrik Norum, Ellen Skarpen, Andreas Brech, Raoul Kuiper, Jo Waaler, Stefan Krauss, Therese Sørlie
BACKGROUND: The WNT pathway regulates intestinal stem cells and is frequently disrupted in intestinal adenomas. The pathway contains several potential biotargets for interference, including the poly-ADP ribosyltransferase enzymes tankyrase1 and 2. LGR5 is a known WNT pathway target gene and marker of intestinal stem cells. The LGR5+ stem cells are located in the crypt base and capable of regenerating all intestinal epithelial cell lineages. RESULTS: We treated Lgr5-EGFP-Ires-CreERT2;R26R-Confetti mice with the tankyrase inhibitor G007-LK for up to 3 weeks to assess the effect on duodenal stem cell homeostasis and on the integrity of intestinal epithelium...
January 9, 2018: Biological Research
https://www.readbyqxmd.com/read/29306025/silver-nanoparticles-induce-sh-sy5y-cell-apoptosis-via-endoplasmic-reticulum-and-mitochondrial-pathways-that-lengthen-endoplasmic-reticulum-mitochondria-contact-sites-and-alter-inositol-3-phosphate-receptor-function
#18
Lin Li, Jiahui Cui, Zi Liu, Xuejiao Zhou, Zengqiang Li, Yang Yu, Yuanyuan Jia, Daiying Zuo, Yingliang Wu
Silver nanoparticles (AgNPs) have many medical and commercial applications, but their effects on human health are poorly understood. The aim of this study was to assess the effect of AgNPs on the human neuroblastoma cell line SH-SY5Y and to explore their potential mechanisms of action. We found that AgNPs decreased SH-SY5Y cell viability in a dose- and time-dependent manner. Exposure to AgNPs activated endoplasmic reticulum (ER) stress, as reflected by upregulated expression of glucose-regulated protein 78 (GRP78), phosphorylated PKR-like endoplasmic reticulum kinase (p-PERK), phosphorylated eukaryotic translation initiation factor 2α (p-eIF2α), C/EBP homology protein (CHOP), spliced X-box binding protein-1 (XBP1), and phosphorylated inositol-requiring enzyme (p-IRE), all of which are involved in the cellular unfolded protein response...
January 3, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29305201/initial-assessment-of-the-efficacy-of-irreversible-electroporation-ire-in-the-focal-treatment-of-localised-renal-cell-carcinoma-rcc-with-delayed-interval-kidney-tumour-resection-irene-trial-an-ablate-and-resect-pilot-study
#19
Johann J Wendler, Maciej Pech, Frank Fischbach, Julian Jürgens, Björn Friebe, Daniel Baumunk, Markus Porsch, Simon Blaschke, Daniel Schindele, Sandra Siedentopf, Jens Ricke, Martin Schostak, Jens Köllermann, Uwe B Liehr
OBJECTIVES: To assess the efficacy of irreversible electroporation (IRE) ablation of pT1a renal-cell carcinoma (RCC) in the first prospective, monocentric Phase 2a pilot ablate-and-resect study (IRENE trial). It has been postulated that focal IRE can bring about complete ablation of soft-tissue tumours with protection of healthy peritumoral tissue and anatomical structures. METHODS: The first seven study patients, with biopsy-proven pT1a RCC (15-39mm) underwent IRE...
January 2, 2018: Urology
https://www.readbyqxmd.com/read/29305038/intentional-switching-in-auditory-selective-attention-exploring-attention-shifts-with-different-reverberation-times
#20
Josefa Oberem, Julia Seibold, Iring Koch, Janina Fels
Using a well-established binaural-listening paradigm the ability to intentionally switch auditory selective attention was examined under anechoic, low reverberation (0.8 s) and high reverberation (1.75 s) conditions. Twenty-three young, normal-hearing subjects were tested in a within-subject design to analyze influences of the reverberation times. Spoken word pairs by two speakers were presented simultaneously to subjects from two of eight azimuth positions. The stimuli consisted of a single number word, (i...
December 22, 2017: Hearing Research
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