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https://www.readbyqxmd.com/read/28732031/breast-tissue-ablation-with-irreversible-electroporation-in-rabbits-a-safety-and-feasibility-study
#1
Wenlong Zhang, Wanning Wang, Wei Chai, Xiaomei Luo, Jiannan Li, Jian Shi, Liqi Bi, Lizhi Niu
BACKGROUND AND AIM: Irreversible electroporation (IRE) was confirmed to control several solid tumors effectively in vivo. Our preclinical study aimed to assess the feasibility and safety of IRE in the breast of rabbit. METHODS: Thirty New Zealand white rabbits were randomly divided into 3 groups of 10 rabbits (control group, IRE group A, and B). Two mono-electrode needles were inserted into the breast tissue by percutaneous puncture. Electrocardiogram and vital signs were monitored before, during, and after ablation...
2017: PloS One
https://www.readbyqxmd.com/read/28727292/impact-of-signal-peptides-on-furin-2a-mediated-monoclonal-antibody-secretion-in-cho-cells
#2
Jian'er Lin, Shu Hui Neo, Steven C L Ho, Jessna H M Yeo, Tianhua Wang, Wei Zhang, Xuezhi Bi, Sheng-Hao Chao, Yuansheng Yang
Studies had shown the benefits of using furin-2A peptides for high monoclonal antibody (mAb) expression in mammalian cells. How signal peptides affect furin-2A mediated mAb secretion has yet to be investigated. Here we evaluated the impact of signal peptides on mAb secretion in furin-2A based tricistronic vectors in CHO cells. In each tricistronic vector, heavy chain (HC) is arranged as the first cistron and followed by a furin recognition sequence, a 2A peptide, light chain (LC), an internal ribosome entry site (IRES), and dihydrofolate reductase (DHFR)...
July 20, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28722796/extended-catalytic-scope-of-a-well-known-enzyme-asymmetric-reduction-of-iminium-substrates-by-glucose-dehydrogenase
#3
Sebastian Roth, Andreas Präg, Cindy Wechsler, Marija Marolt, Sascha Ferlaino, Steffen Lüdeke, Nicolas Sandon, Dennis Wetzl, Hans Iding, Beat Wirz, Michael Müller
NADP(H)-dependent imine reductases (IREDs) are of interest in biocatalytic research due to their ability to generate chiral amines from imine/iminium substrates. In reaction protocols involving IREDs, glucose dehydrogenase (GDH) is generally used to regenerate the expensive cofactor NADPH by oxidation of d-glucose to gluconolactone. We have characterized different IREDs with regard to reduction of a set of bicyclic iminium compounds and have utilized (1) H NMR and GC analyses to determine degree of substrate conversion and product enantiomeric excess (ee)...
July 19, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28721494/a-comprehensive-characterization-of-parameters-affecting-high-frequency-irreversible-electroporation-lesions
#4
Tyler Miklovic, Eduardo L Latouche, Matthew R DeWitt, Rafael V Davalos, Michael B Sano
Several focal therapies are being investigated clinically to treat tumors in which surgery is contraindicated. Many of these ablation techniques, such as radiofrequency ablation and microwave ablation, rely on thermal damage mechanisms which can put critical nerves or vasculature at risk. Irreversible electroporation (IRE) is a minimally invasive, non-thermal technique to destroy tumors. A series of short electric pulses create nanoscale defects in the cell membrane, eventually leading to cell death. Typical IRE protocols deliver a series of 50-100 µs monopolar pulses...
July 18, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28710293/probing-gata-factor-function-in-mouse-leydig-cells-via-testicular-injection-of-adenoviral-vectors
#5
Gervette M Penny, Rebecca B Cochran, Marjut Pihlajoki, Antti Kyrönlahti, Anja Schrade, Merja Häkkinen, Jorma Toppari, Markku Heikinheimo, David B Wilson
Testicular Leydig cells produce androgens essential for proper male reproductive development and fertility. Here, we describe a new Leydig cell ablation model based on Cre/Lox recombination of mouse Gata4 and Gata6, two genes implicated in the transcriptional regulation of steroidogenesis. The testicular interstitium of adult Gata4(flox/flox);Gata6(flox/flox) mice was injected with adenoviral vectors encoding Cre + GFP (Ad-Cre-IRES-GFP) or GFP alone (Ad-GFP). The vectors efficiently and selectively transduced Leydig cells, as evidenced by GFP reporter expression...
July 14, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28700206/drug-porous-silicon-dual-luminescent-system-for-monitoring-and-inhibition-of-wound-infection
#6
Xisheng Chen, Fangjie Wo, Yao Jin, Jie Tan, Yan Lai, Jianmin Wu
Wound monitoring and curing is of great importance in biomedical research. This work created a smart bandage that can simultaneously monitor and inhibit wound infection. The main components of the smart bandage are luminescent porous silicon (LuPSi) particles loaded with ciprofloxacin (CIP). This dual luminescent system can undergo accelerated fluorescent color change from red to blue upon the stimulation of reactive oxygen species (ROS) and elevated pH, which are main biomarkers in the infected wound. The mechanism behind the chemical-triggered fluorescent color change was studied in detail...
July 17, 2017: ACS Nano
https://www.readbyqxmd.com/read/28699869/hepatitis-c-virus-in-vitro-replication-is-efficiently-inhibited-by-acridone-fac4
#7
Guilherme Rodrigues Fernandes Campos, Cíntia Bittar, Ana Carolina Gomes Jardim, Jacqueline Farinha Shimizu, Mariana Nogueira Batista, Eder Ramos Paganini, Letícia Ribeiro de Assis, Christopher Bartlett, Mark Harris, Vanderlan da Silva Bolzani, Luis Octavio Regasini, Paula Rahal
Hepatitis C virus (HCV) affects about 170 million people worldwide. The current treatment has a high cost and variable response rates according to the virus genotype. Acridones, a group of compounds extracted from natural sources, showed potential antiviral actions against HCV. Thus, this study aimed to evaluate the effect of a panel of 14 synthetic acridones on the HCV life cycle. The compounds were screened using an Huh7.5 cell line stably harbouring the HCV genotype 2a subgenomic replicon SGR-Feo-JFH-1...
July 12, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28699509/combination-of-multifunctional-nanoparticles-and-interventional-techniques-makes-each-other-going-further-in-the-field-of-cancer-treatment
#8
Haibo Shao, Tengchuang Ma, Duo Hong, Wenhan Xu, Shuai Li, Ronald S Arellano, Ke Xu
Nano-oncology and interventional oncology are both rapidly emerging fields in loco-regional cancer therapies. Synergistic combination of both fields offers drastic improvement in performance and efficacy of cancer killings. Interventional techniques such selective arterial catheterization, irreversible electroporation (IRE) and radiofrequency ablation (RFA) had dramatically enhance the cancer targetability and anticancer efficacy of nanoparticles (NPs). Furthermore, there is synergistic effect by using different interventional techniques together on NPs assisted cancer treatment...
July 10, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/28695960/galectin-1-enhances-the-generation-of-neural-crest-cells
#9
Tsutomu Motohashi, Masahiro Nishioka, Daisuke Kitagawa, Norito Kawamura, Natsuki Watanabe, Takanori Wakaoka, Toshihiko Kadoya, Takahiro Kunisada
Neural crest (NC) cells are multipotent cells that emerge from the dorsal region of the neural tube. After delaminating from the neural tube, NC cells migrate throughout the developing embryo and differentiate into various cells: neurons and glial cells of the peripheral nervous system, melanocytes of skin, and skeletal elements of the face and head. We previously analyzed the gene expression profile of a NC subpopulation isolated from Sox10-IRES-Venus mice and found that the carbohydrate-binding protein, Galectin-1 (Gal-1) was strongly expressed in generating NC cells...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28687047/the-role-of-irreversible-electroporation-ire-for-locally-advanced-pancreatic-cancer-a-systematic-review-of-safety-and-efficacy
#10
Daniel Ansari, Stina Kristoffersson, Roland Andersson, Magnus Bergenfeldt
OBJECTIVES: Irreversible electroporation (IRE) is a new modality for tumor ablation. Electrodes are placed around the tumor, and a pulsed, direct current with a field strength of 2000 V/cm is delivered. The direct current drives cells into apoptosis and cell death without causing significant heating of the tissues, which spares the extracellular matrix and proteins. The purpose of this review was to evaluate current experience of IRE for the ablation of pancreatic cancer. MATERIAL AND METHODS: We searched PubMed for all studies of IRE in human pancreatic cancer in English reporting at least 10 patients...
July 7, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28684008/ribosomal-chamber-music-toward-an-understanding-of-ires-mechanisms
#11
REVIEW
Hiroshi Yamamoto, Anett Unbehaun, Christian M T Spahn
Internal initiation is a 5'-end-independent mode of translation initiation engaged by many virus- and putatively some cell-encoded templates. Internal initiation is facilitated by specific RNA tertiary folds, called internal ribosomal entry sites (IRESs), in the 5' untranslated region (UTR) of the respective transcripts. In this review we discuss recent structural insight into how established IRESs first capture and then manipulate the eukaryotic translation machinery through non-canonical interactions and by guiding the intrinsic conformational flexibility of the eukaryotic ribosome...
July 3, 2017: Trends in Biochemical Sciences
https://www.readbyqxmd.com/read/28677092/amelioration-of-bleomycin-induced-pulmonary-fibrosis-by-chlorogenic-acid-through-endoplasmic-reticulum-stress-inhibition
#12
Yi-Chun Wang, Jing Dong, Jing Nie, Ji-Xiang Zhu, Hui Wang, Qiong Chen, Jun-Yi Chen, Jia-Mei Xia, Wei Shuai
To investigate the inhibitory effects of chlorogenic acid on pulmonary fibrosis and the internal mechanisms in vivo and in vitro. 30 male BALB/C mice were randomized into 5 groups: control group, pulmonary fibrosis model group, low, middle and high dose of chlorogenic acid groups. Mice in pulmonary fibrosis model group were administered 5.0 mg/kg bleomycin with intracheal instillation and mice in 3 chlorogenic acid groups were treated with chlorogenic acid every day for 28 days after bleomycin administration...
July 4, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28675694/benzyl-isothiocyanate-bitc-induces-apoptosis-of-gbm-8401-human-brain-glioblastoma-multiforms-cells-via-activation-of-caspase-8-bid-and-the-reactive-oxygen-species-dependent-mitochondrial-pathway
#13
Hung-Sheng Shang, Yung-Luen Shih, Tai-Jung Lu, Ching-Hsiao Lee, Shu-Ching Hsueh, Yu-Cheng Chou, Hsu-Feng Lu, Nien-Chieh Liao, Jing-Gung Chung
Benzyl isothiocyanate (BITC) is one of member of the isothiocyanate family which has been shown to induce cancer cell apoptosis in many human cancer cells. In the present study, we investigated the effects of BITC on the growth of GBM 8401 human brain glioblastoma multiforms cells. Results indicated that BITC-induced cell morphological changes decreased in the percentage of viable GBM8401 cells and these effects are dose-dependent manners. Results from flow cytometric assay indicated that BITC induced sub-G1 phase and induction of apoptosis of GBM 8401 cells...
December 2016: Environmental Toxicology
https://www.readbyqxmd.com/read/28674519/somatostatin-ires-cre-mice-between-knockout-and-wild-type
#14
Cécile Viollet, Axelle Simon, Virginie Tolle, Alexandra Labarthe, Dominique Grouselle, Yann Loe-Mie, Michel Simonneau, Guillaume Martel, Jacques Epelbaum
The neuropeptide somatostatin (SOM) is widely expressed in rodent brain and somatostatin-IRES-Cre (SOM-cre) mouse strains are increasingly used to unravel the physiology of SOM-containing neurons. However, while knock-in targeting strategy greatly improves Cre-Lox system accuracy, recent reports have shown that genomic insertion of Cre construct per se can markedly affect physiological function. We show that Cre transgene insertion into the 3'UTR of the somatostatin gene leads to the selective and massive depletion of endogenous SOM in all tested brain regions...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28672987/evaluation-of-the-safety-of-irreversible-electroporation-on-the-stomach-wall-using-a-pig-model
#15
Jiannan Li, Jianying Zeng, Jibing Chen, Jian Shi, Xiaomei Luo, Gang Fang, Wei Chai, Wenlong Zhang, Tongjun Liu, Lizhi Niu
The aim of the present study was to evaluate the effects of irreversible electroporation (IRE) on the stomach wall following the direct application of IRE onto the organ surface. IRE ablation was performed in 8 Tibetan mini-pigs, which were randomly assigned into two groups based on their ablated areas: Group A, gastric cardia, fundus of stomach, gastric body and group B, lesser gastric curvature, greater gastric curvature, stomach pylorus. Two IRE needles were placed in the space between the stomach wall and the liver (not inserted into the stomach tissue), and three lesions were created in each pig...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28662065/monoclonal-antibodies-expression-improvement-in-cho-cells-by-piggybac-transposition-regarding-vectors-ratios-and-design
#16
Samira Ahmadi, Fatemeh Davami, Noushin Davoudi, Fatemeh Nematpour, Maryam Ahmadi, Saeedeh Ebadat, Kayhan Azadmanesh, Farzaneh Barkhordari, Fereidoun Mahboudi
Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Transposons are useful devices to target active parts of genomes, and PiggyBac (PB) transposon can be considered as a good option. In the present study, three PB transposon donor vectors containing both heavy and light chains were constructed, one contained independent expression cassettes while the others utilized either an Internal Ribosome Entry Site (IRES) or 2A element to express mAb...
2017: PloS One
https://www.readbyqxmd.com/read/28661522/tubulogenesis-of-co-cultured-human-ips-derived-endothelial-cells-and-human-mesenchymal-stem-cells-in-fibrin-and-gelatin-methacrylate-gels
#17
G A Calderon, P Thai, C W Hsu, B Grigoryan, S M Gibson, M E Dickinson, J S Miller
Here, we investigate the tubulogenic potential of commercially-sourced iPS-ECs with and without supporting commercially-sourced hMSCs within 3D natural fibrin or semi-synthetic gelatin methacrylate (GelMA) hydrogels. We developed a selectable dual color third generation lentiviral reporter (hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puro) to differentially label the nucleus and cytoplasm of iPS-ECs which allowed real-time tracking of key steps of vascular morphogenesis such as vacuole formation and coalescence to form shared multicellular lumens...
June 29, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28652112/multinational-retrospective-case-control-study-of-risk-factors-for-the-development-of-late-invasive-pulmonary-aspergillosis-following-kidney-transplantation
#18
Francisco López-Medrano, Mario Fernández-Ruiz, José Tiago Silva, Peggy L Carver, Christian van Delden, Esperanza Merino, María José Pérez-Saez, Milagros Montero, Julien Coussement, Milene de Abreu Mazzolin, Carlos Cervera, Lidia Santos, Nuria Sabé, Anne Scemla, Elisa Cordero, Leónidas Cruzado-Vega, Paloma Leticia Martín-Moreno, Óscar Len, Eddison Rudas, Alfredo Ponce de León, Mariano Arriola, Ricardo Lauzurica, Miruna D David, Claudia González-Rico, Fernando Henríquez-Palop, Jesús Fortún, Marcio Nucci, Oriol Manuel, José Ramón Paño-Pardo, Miguel Montejo, Antonio Vena, Beatriz Sánchez-Sobrino, Auxiliadora Mazuecos, Julio Pascual, Juan Pablo Horcajada, Thanh Lecompte, Asunción Moreno, Jordi Carratalà, Marino Blanes, Domingo Hernández, Erick Alejandro Hernández-Méndez, María Carmen Fariñas, Manuel Perelló-Carrascosa, Patricia Muñoz, Amado Andrés, José María Aguado
OBJECTIVES: To assess the risk factors for the development of late-onset invasive pulmonary aspergillosis (IPA) after kidney transplantation (KT). METHODS: We performed a multinational case-control study that retrospectively recruited 112 KT recipients diagnosed with IPA between 2000 and 2013. Controls were matched (1:1 ratio) by center and date of transplantation. Immunosuppression-related events (IREs) included the occurrence of non-ventilator-associated pneumonia, tuberculosis, cytomegalovirus disease and/or de novo malignancy...
June 23, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28643305/-interventional-therapy-of-colorectal-liver-metastasis
#19
Qi Zhang, Gaojun Teng
Colorectal liver metastasis (CRLM) is one of the most difficult and key points in the treatment of colorectal cancer. Approximately 50% to 60% of patients diagnosed as colorectal cancer develops metastasis, and 80% to 90% of CRLM is unresectable. Surgical resection is the first-line treatment for CRLM, while it is only suitable for about 15% patients. Systemic chemotherapy can prolong the survival of CRLM patients, however, a part of CRLM patients are resistant to chemotherapy. With the development of technology and the update of clinical evidence, individual therapy with target drugs and multidisciplinary treatment (MDT) have became a tendency, and minimally invasive interventional therapy has gained more acceptance in the MDT mode of the treatment for CRLM...
June 25, 2017: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/28642716/effects-of-acute-hypoxia-and-reoxygenation-on-physiological-and-immune-responses-and-redox-balance-of-wuchang-bream-megalobrama-amblycephala-yih-1955
#20
Nan Chen, Meng Wu, Guo-Pan Tang, Hui-Juan Wang, Chun-Xiao Huang, Xin-Jie Wu, Yan He, Bao Zhang, Cui-Hong Huang, Hong Liu, Wei-Min Wang, Huan-Ling Wang
To study Megalobrama amblycephala adaption to water hypoxia, the changes in physiological levels, innate immune responses, redox balance of M.amblycephala during hypoxia were investigated in the present study. When M. amblycephala were exposed to different dissolved oxygen (DO) including control (DO: 5.5 mg/L) and acute hypoxia (DO: 3.5 and 1.0 mg/L, respectively), hemoglobin (Hb), methemoglobin (MetHb), glucose, Na(+), succinatedehydrogenase (SDH), lactate, interferon alpha (IFNα), and lysozyme (LYZ), except hepatic glycogen and albumin gradually increased with the decrease of DO level...
2017: Frontiers in Physiology
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