keyword
MENU ▼
Read by QxMD icon Read
search

double negative T cell

keyword
https://www.readbyqxmd.com/read/29662616/recruitment-of-lysine-demethylase-2a-to-dna-double-strand-breaks-and-its-interaction-with-53bp1-ensures-genome-stability
#1
Murilo T D Bueno, Marta Baldascini, Stéphane Richard, Noel F Lowndes
Lysine demethylase 2A (KDM2A) functions in transcription as a demethylase of lysine 36 on histone H3. Herein, we characterise a role for KDM2A in the DNA damage response in which KDM2A stimulates conjugation of ubiquitin to 53BP1. Impaired KDM2A-mediated ubiquitination negatively affects the recruitment of 53BP1 to DSBs. Notably, we show that KDM2A itself is recruited to DSBs in a process that depends on its demethylase activity and zinc finger domain. Moreover, we show that KDM2A plays an important role in ensuring genomic stability upon DNA damage...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29623179/co-expression-of-myc-and-bcl2-predicts-poorer-outcomes-for-relapsed-refractory-diffuse-large-b-cell-lymphoma-with-r-ice-and-intent-to-transplant
#2
Joshua Allen, Ana Lucia Ruano Mendez, Lisa Rybicki, Yazeed Sawalha, Deepa Jagadeesh, Robert Dean, Brad Pohlman, Mitchell R Smith, Eric D Hsi, Brian T Hill
Diffuse large B-cell lymphoma (DLBCL) with co-expression of MYC and BCL2 protein by immunohistochemistry (IHC) - that is, double-expresser lymphoma (DEL) - is associated with poor outcomes after standard frontline therapy. Less is known about the prognostic impact of DEL in patients with relapsed/refractory disease treated with salvage therapy and autologous stem cell transplantation (ASCT). We analyzed the outcomes of 167 patients with relapsed/refractory DLBCL treated with R-ICE (rituximab, ifosfamide, carboplatin and etoposide), of whom 111 patients (66%) underwent ASCT...
April 2018: Therapeutic Advances in Hematology
https://www.readbyqxmd.com/read/29618724/global-dynamics-of-stage-specific-transcription-factor-binding-during-thymocyte-development
#3
Tomonori Hosoya, Ricardo D'Oliveira Albanus, John Hensley, Greggory Myers, Yasuhiro Kyono, Jacob Kitzman, Stephen C J Parker, James Douglas Engel
In vertebrates, multiple transcription factors (TFs) bind to gene regulatory elements (promoters, enhancers, and silencers) to execute developmental expression changes. ChIP experiments are often used to identify where TFs bind to regulatory elements in the genome, but the requirement of TF-specific antibodies hampers analyses of tens of TFs at multiple loci. Here we tested whether TF binding predictions using ATAC-seq can be used to infer the identity of TFs that bind to functionally validated enhancers of the Cd4, Cd8, and Gata3 genes in thymocytes...
April 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29595533/ido1-expression-is-associated-with-immune-tolerance-and-poor-prognosis-in-patients-with-surgically-resected-esophageal-cancer
#4
Yuki Kiyozumi, Yoshifumi Baba, Kazuo Okadome, Taisuke Yagi, Takatsugu Ishimoto, Masaaki Iwatsuki, Yuji Miyamoto, Naoya Yoshida, Masayuki Watanabe, Yoshihiro Komohara, Hideo Baba
OBJECTIVES: To evaluate the relationship between indoleamine 2, 3-dioxygenase (IDO1) expression and tumoral immune status and clinical outcome in esophageal cancer. SUMMARY BACKGROUND DATA: IDO1 is a primary enzyme that generates immunosuppressive metabolites such as tryptophan and kynurenine. Like the PD-1/PD-L1 pathway, IDO1 plays a major role in tumor immunology and is a potential immune-based therapeutic target. METHODS: The expressions of IDO1, CD8 (a marker of cytotoxic T cells), FOXP3 [a marker of regulatory T cells (Treg)], and PD-L1 in 305 curatively resected esophageal cancers were evaluated by immunostaining...
March 27, 2018: Annals of Surgery
https://www.readbyqxmd.com/read/29595510/safety-and-immunogenicity-of-the-novel-tuberculosis-vaccine-id93-gla-se-in-bcg-vaccinated-healthy-adults-in-south-africa-a-randomised-double-blind-placebo-controlled-phase-1-trial
#5
Adam Penn-Nicholson, Michele Tameris, Erica Smit, Tracey A Day, Munyaradzi Musvosvi, Lakshmi Jayashankar, Julie Vergara, Simbarashe Mabwe, Nicole Bilek, Hendrik Geldenhuys, Angelique Kany-Kany Luabeya, Ruth Ellis, Ann M Ginsberg, Willem A Hanekom, Steven G Reed, Rhea N Coler, Thomas J Scriba, Mark Hatherill
BACKGROUND: A vaccine that prevents pulmonary tuberculosis in adults is needed to halt transmission in endemic regions. This trial aimed to assess the safety and immunogenicity of three administrations at varying doses of antigen and adjuvant of an investigational vaccine (ID93 + GLA-SE) compared with placebo in previously BCG-vaccinated healthy adults in a tuberculosis endemic country. METHODS: In this randomised, double-blind, placebo-controlled phase 1 trial, we enrolled HIV-negative, previously BCG-vaccinated adults (aged 18-50 years), with no evidence of previous or current tuberculosis disease, from among community volunteers in the Worcester region of Western Cape, South Africa...
April 2018: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/29556720/polymyxin-b-causes-dna-damage-in-hk-2-cells-and-mice
#6
B Yun, T Zhang, M A K Azad, J Wang, C J Nowell, P Kalitsis, T Velkov, D F Hudson, J Li
Increasing incidence of multidrug-resistant bacteria presents an imminent risk to global health. Polymyxins are 'last-resort' antibiotics against Gram-negative 'superbugs'; however, nephrotoxicity remains a key impediment in their clinical use. Molecular mechanisms underlying this nephrotoxicity remain poorly defined. Here, we examined the pathways which led to polymyxin B induced cell death in vitro and in vivo. Human proximal tubular cells were treated with polymyxin B (12.5-100 μM) for up to 24 h and showed a significant increase in micronuclei frequency, as well as abnormal mitotic events (over 40% in treated cells, p < 0...
March 20, 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29551338/sirolimus-in-patients-with-clinically-active-systemic-lupus-erythematosus-resistant-to-or-intolerant-of-conventional-medications-a-single-arm-open-label-phase-1-2-trial
#7
Zhi-Wei Lai, Ryan Kelly, Thomas Winans, Ivan Marchena, Ashwini Shadakshari, Julie Yu, Maha Dawood, Ricardo Garcia, Hajra Tily, Lisa Francis, Stephen V Faraone, Paul E Phillips, Andras Perl
BACKGROUND: Patients with systemic lupus erythematosus have T-cell dysfunction that has been attributed to the activation of the mammalian target of rapamycin (mTOR). Rapamycin inhibits antigen-induced T-cell proliferation and has been developed as a medication under the generic designation of sirolimus. We assessed safety, tolerance, and efficacy of sirolimus in a prospective, biomarker-driven, open-label clinical trial. METHODS: We did a single-arm, open-label, phase 1/2 trial of sirolimus in patients with active systemic lupus erythematosus disease unresponsive to, or intolerant of, conventional medications at the State University of New York Upstate Medical University (Syracuse, NY, USA)...
March 15, 2018: Lancet
https://www.readbyqxmd.com/read/29482663/impaired-lymph-node-stromal-cell-function-during-the-earliest-phases-of-rheumatoid-arthritis
#8
Janine S Hähnlein, Reza Nadafi, Tineke de Jong, Tamara H Ramwadhdoebe, Johanna F Semmelink, Karen I Maijer, IJsbrand A Zijlstra, Mario Maas, Danielle M Gerlag, Teunis B H Geijtenbeek, Paul P Tak, Reina E Mebius, Lisa G M van Baarsen
BACKGROUND: Systemic autoimmunity can be present years before clinical onset of rheumatoid arthritis (RA). Adaptive immunity is initiated in lymphoid tissue where lymph node stromal cells (LNSCs) regulate immune responses through their intimate connection with leucocytes. We postulate that malfunctioning of LNSCs creates a microenvironment in which normal immune responses are not properly controlled, possibly leading to autoimmune disease. In this study we established an experimental model for studying the functional capacities of human LNSCs during RA development...
February 26, 2018: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29466755/transformation-of-accessible-chromatin-and-3d-nucleome-underlies-lineage-commitment-of-early-t-cells
#9
Gangqing Hu, Kairong Cui, Difeng Fang, Satoshi Hirose, Xun Wang, Darawalee Wangsa, Wenfei Jin, Thomas Ried, Pentao Liu, Jinfang Zhu, Ellen V Rothenberg, Keji Zhao
How chromatin reorganization coordinates differentiation and lineage commitment from hematopoietic stem and progenitor cells (HSPCs) to mature immune cells has not been well understood. Here, we carried out an integrative analysis of chromatin accessibility, topologically associating domains, AB compartments, and gene expression from HSPCs to CD4+ CD8+ T cells. We found that abrupt genome-wide changes at all three levels of chromatin organization occur during the transition from double-negative stage 2 (DN2) to DN3, accompanying the T lineage commitment...
February 20, 2018: Immunity
https://www.readbyqxmd.com/read/29466409/il-7-treatment-augments-and-prolongs-sepsis-induced-expansion-of-il-10-producing-b-lymphocytes-and-myeloid-derived-suppressor-cells
#10
Upasana Kulkarni, Christoph Herrmenau, Stephanie J Win, Michael Bauer, Thomas Kamradt
Immunological dysregulation in sepsis is associated with often lethal secondary infections. Loss of effector cells and an expansion of immunoregulatory cell populations both contribute to sepsis-induced immunosuppression. The extent and duration of this immunosuppression are unknown. Interleukin 7 (IL-7) is important for the maintenance of lymphocytes and can accelerate the reconstitution of effector lymphocytes in sepsis. How IL-7 influences immunosuppressive cell populations is unknown. We have used the mouse model of peritoneal contamination and infection (PCI) to investigate the expansion of immunoregulatory cells as long-term sequelae of sepsis with or without IL-7 treatment...
2018: PloS One
https://www.readbyqxmd.com/read/29462456/n-acetylcysteine-in-a-double-blind-randomized-placebo-controlled-trial-toward-biomarker-guided-treatment-in-early-psychosis
#11
Philippe Conus, Larry J Seidman, Margot Fournier, Lijing Xin, Martine Cleusix, Philipp S Baumann, Carina Ferrari, Ann Cousins, Luis Alameda, Mehdi Gholam-Rezaee, Philippe Golay, Raoul Jenni, T-U Wilson Woo, Matcheri S Keshavan, Chin B Eap, Joanne Wojcik, Michel Cuenod, Thierry Buclin, Rolf Gruetter, Kim Q Do
Biomarker-guided treatments are needed in psychiatry, and previous data suggest oxidative stress may be a target in schizophrenia. A previous add-on trial with the antioxidant N-acetylcysteine (NAC) led to negative symptom reductions in chronic patients. We aim to study NAC's impact on symptoms and neurocognition in early psychosis (EP) and to explore whether glutathione (GSH)/redox markers could represent valid biomarkers to guide treatment. In a double-blind, randomized, placebo-controlled trial in 63 EP patients, we assessed the effect of NAC supplementation (2700 mg/day, 6 months) on PANSS, neurocognition, and redox markers (brain GSH [GSHmPFC], blood cells GSH levels [GSHBC], GSH peroxidase activity [GPxBC])...
February 15, 2018: Schizophrenia Bulletin
https://www.readbyqxmd.com/read/29453859/diminished-cxcr5-expression-in-peripheral-blood-of-patients-with-sj%C3%A3-gren-s-syndrome-may-relate-to-both-genotype-and-salivary-gland-homing
#12
Lara A Aqrawi, Margarita Ivanchenko, Albin Björk, Jorge I Ramírez Sepúlveda, Juliana Imgenberg-Kreuz, Marika Kvarnström, Philipp Haselmayer, Janicke Liaaen Jensen, Gunnel Nordmark, Karine Chemin, Kathrine Skarstein, Marie Wahren-Herlenius
Genetic investigations of Sjögren's syndrome (SS) have identified a susceptibility locus at p23.3 of chromosome 11, which contains the CXCR5 gene. CXCR5 is a chemokine receptor expressed on B and T cell subsets, and binds the chemotactic ligand CXCL13. We here aimed to link the genetic association with functional effects and explore the CXCR5/CXCL13 axis in SS. Expression quantitative trait loci analysis of the 11q23.3 locus was performed using B cell mRNA expression data from genotyped individuals. Lymphocyte surface markers were assessed by flow cytometry, and CXCL13 levels by a proximity extension assay...
February 17, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29448960/double-negative-cd27-igd-b-cells-are-expanded-in-nsclc-and-inversely-correlate-with-affinity-matured-b-cell-populations
#13
Sara M Centuori, Cecil J Gomes, Samuel S Kim, Charles W Putnam, Brandon T Larsen, Linda L Garland, David W Mount, Jesse D Martinez
BACKGROUND: The presence of B cells in early stage non-small cell lung cancer (NSCLC) is associated with longer survival, however, the role these cells play in the generation and maintenance of anti-tumor immunity is unclear. B cells differentiate into a variety of subsets with differing characteristics and functions. To date, there is limited information on the specific B cell subsets found within NSCLC. To better understand the composition of the B cell populations found in NSCLC we have begun characterizing B cells in lung tumors and have detected a population of B cells that are CD79A + CD27 - IgD - ...
February 15, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29444269/association-of-angiotensin-ii-type-1-receptor-antibodies-with-graft-histology-function-and-survival-in-paediatric-renal-transplant-recipients
#14
Alexander Fichtner, Caner Süsal, Claudia Schröder, Britta Höcker, Susanne Rieger, Rüdiger Waldherr, Jens H Westhoff, Anja Sander, Duska Dragun, Burkhard Tönshoff
Background: We analysed in a carefully phenotyped cohort of paediatric patients the association of serum angiotensin II type 1 receptor antibodies (AT1R-Ab) with specific histological lesions and with graft function and survival in conjunction with overall and complement-binding donor-specific human leucocyte antigen donor-specific antibodies (HLA-DSA). Methods: Sera of 62 patients at the time of renal graft biopsy for clinical indication >1 year post-transplant were assessed for AT1R-Ab by enzyme-linked immunosorbent assay (ELISA) and for DSA and C1q-fixing DSA by single-antigen bead technology...
February 12, 2018: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29434594/p38%C3%AE-and-p38%C3%AE-are-involved-in-t-lymphocyte-development
#15
Ana Risco, Miguel A Martin-Serrano, Domingo F Barber, Ana Cuenda
p38 mitogen-activated protein kinase (MAPK) signal transduction pathways are essential regulators of the immune response. Particularly, p38γ and p38δ regulate many immune cell functions such as cytokine production, migration, or T cell activation; however, their involvement in immune cell development is largely unknown. Here, we analysed the role of p38 MAPK isoforms p38γ and p38δ in T cell differentiation in the thymus and in lymph nodes, using mice deficient in p38γ, p38δ, or in both. We found that the T cell differentiation program in the thymus was affected at different stages in p38γ-, p38δ-, and p38γ/δ-deficient mice, and also peripheral T cell homaeostasis was compromised...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29433222/segregation-of-tetragenococcus-halophilus-and-zygosaccharomyces-rouxii-using-w1-o-w2-double-emulsion-for-use-in-mixed-culture-fermentation
#16
Putu Virgina Partha Devanthi, Hani El Kadri, Allen Bowden, Fotios Spyropoulos, Konstantinos Gkatzionis
Antagonism in mixed culture fermentation can result in undesirable metabolic activity and negatively affect the fermentation process. Water-oil-water (W1/O/W2) double emulsions (DE) could be utilized in fermentation for segregating multiple species and controlling their release and activity. Zygosaccharomyces rouxii and Tetragenococcus halophilus, two predominant microbial species in soy sauce fermentation, were incorporated in the internal W1 and external W2 phase of a W1/O/W2, respectively. The suitability of DE for controlling T...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29431868/correction-of-t-cell-deficiency-in-zap-70-knockout-mice-by-simple-intraperitoneal-adoptive-transfer-of-thymocytes
#17
Réka Kugyelka, Zoltán Kohl, Katalin Olasz, Lilla Prenek, Tímea Berki, Péter Balogh, Ferenc Boldizsár
The tyrosine kinase ZAP-70 (zeta-chain associated protein of 70 kDa) plays a key role in T cell development and signaling. In the absence of ZAP-70, T cell development is arrested in the CD4+ CD8+ double positive stage, thus ZAP-70 homozygous knockout (ZAP-70-/- ) mice have no mature T cells in their peripheral lymphoid organs and blood, causing severe immunodeficiency. We investigated the early kinetics and long-term effects of wild-type thymocyte transfer on T cell repopulation in ZAP-70-/- mice. We used a single intraperitoneal (ip...
February 12, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29429572/mutations-in-the-baf-complex-subunit-dpf2-are-associated-with-coffin-siris-syndrome
#18
Georgia Vasileiou, Silvia Vergarajauregui, Sabine Endele, Bernt Popp, Christian Büttner, Arif B Ekici, Marion Gerard, Nuria C Bramswig, Beate Albrecht, Jill Clayton-Smith, Jenny Morton, Susan Tomkins, Karen Low, Astrid Weber, Maren Wenzel, Janine Altmüller, Yun Li, Bernd Wollnik, George Hoganson, Maria-Renée Plona, Megan T Cho, Christian T Thiel, Hermann-Josef Lüdecke, Tim M Strom, Eduardo Calpena, Andrew O M Wilkie, Dagmar Wieczorek, Felix B Engel, André Reis
Variants affecting the function of different subunits of the BAF chromatin-remodelling complex lead to various neurodevelopmental syndromes, including Coffin-Siris syndrome. Furthermore, variants in proteins containing PHD fingers, motifs recognizing specific histone tail modifications, have been associated with several neurological and developmental-delay disorders. Here, we report eight heterozygous de novo variants (one frameshift, two splice site, and five missense) in the gene encoding the BAF complex subunit double plant homeodomain finger 2 (DPF2)...
February 5, 2018: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29428806/tcr%C3%AE-%C3%AE-cd3-cd4-cd8-double-negative-t-cells-in-autoimmunity
#19
REVIEW
D Brandt, C M Hedrich
TCRαβ+CD3+CD4-CD8-"double negative" (DN) T cells comprise a small subset of mature peripheral T cells. The origin and function of DN T cells are somewhat unclear and discussed controversially. While DN T cells resemble a rare and heterogeneous T cell subpopulation in healthy individuals, numbers of TCRαβ+DN T cells are expanded in several inflammatory conditions, where they also exhibit distinct effector phenotypes and infiltrate inflamed tissues. Thus, DN T cells may be involved in systemic inflammation and tissue damage in autoimmune/inflammatory conditions, including SLE, Sjögren's syndrome, and psoriasis...
February 8, 2018: Autoimmunity Reviews
https://www.readbyqxmd.com/read/29421624/comprehensive-overview-of-murine-epididymal-mononuclear-phagocytes-and-lymphocytes-unexpected-populations-arise
#20
Allison Voisin, Marjorie Whitfield, Christelle Damon-Soubeyrand, Chantal Goubely, Joëlle Henry-Berger, Fabrice Saez, Ayhan Kocer, Joël R Drevet, Rachel Guiton
Despite increasing evidence that epididymal immune disorders can lead to infertility, the cells and mechanisms underlying epididymal immunity remain poorly understood. In this study, we propose a rather exhaustive overview of innate and adaptive immune cells present in the murine caput and cauda epididymis. Using flow cytometry and a wide set of markers, we screened the broadest panel of immune cells ever, in this organ. For the first time, we unequivocally quantified the innate populations of monocytes, macrophages, and dendritic cells subtypes...
January 31, 2018: Journal of Reproductive Immunology
keyword
keyword
44184
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"