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vocal fold, regeneration or tissue engineering

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https://www.readbyqxmd.com/read/27619243/tissue-engineering-based-therapeutic-strategies-for-vocal-fold-repair-and-regeneration
#1
REVIEW
Linqing Li, Jeanna M Stiadle, Hang K Lau, Aidan B Zerdoum, Xinqiao Jia, Susan L Thibeault, Kristi L Kiick
Vocal folds are soft laryngeal connective tissues with distinct layered structures and complex multicomponent matrix compositions that endow phonatory and respiratory functions. This delicate tissue is easily damaged by various environmental factors and pathological conditions, altering vocal biomechanics and causing debilitating vocal disorders that detrimentally affect the daily lives of suffering individuals. Modern techniques and advanced knowledge of regenerative medicine have led to a deeper understanding of the microstructure, microphysiology, and micropathophysiology of vocal fold tissues...
November 2016: Biomaterials
https://www.readbyqxmd.com/read/27612519/stem-cell-based-tissue-engineered-laryngeal-replacement
#2
Tahera Ansari, Peggy Lange, Aaron Southgate, Karin Greco, Carla Carvalho, Leanne Partington, Anthony Bullock, Sheila MacNeil, Mark W Lowdell, Paul D Sibbons, Martin A Birchall
: : Patients with laryngeal disorders may have severe morbidity relating to swallowing, vocalization, and respiratory function, for which conventional therapies are suboptimal. A tissue-engineered approach would aim to restore the vocal folds and maintain respiratory function while limiting the extent of scarring in the regenerated tissue. Under Good Laboratory Practice conditions, we decellularized porcine larynges, using detergents and enzymes under negative pressure to produce an acellular scaffold comprising cartilage, muscle, and mucosa...
September 9, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27537192/a-flow-perfusion-bioreactor-system-for-vocal-fold-tissue-engineering-applications
#3
Neda Latifi, Hossein K Heris, Scott L Thomson, Rani Taher, Siavash Kazemirad, Sara Sheibani, Nicole Y K Li-Jessen, Hojatollah Vali, Luc Mongeau
The human vocal folds (VFs) undergo complex biomechanical stimulation during phonation. The aim of the present study was to develop and validate a phono-mimetic VF flow perfusion bioreactor, which mimics the mechanical microenvironment of the human VFs in vitro. The bioreactor uses airflow-induced self-oscillations, which have been shown to produce mechanical loading and contact forces that are representative of human phonation. The bioreactor consisted of two synthetic VF replicas within a silicone body. A cell-scaffold mixture (CSM) consisting of human VF fibroblasts, hyaluronic acid, gelatin, and a polyethylene glycol cross-linker was injected into cavities within the replicas...
September 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27400383/enzymatic-cross-linking-of-resilin-based-proteins-for-vascular-tissue-engineering-applications
#4
Yeji Kim, Emily E Gill, Julie C Liu
Protein-based biomaterials have received significant attention for tissue engineering applications. For example, resilin-based protein gels have been produced with different cross-linking chemistries for applications in cartilage, cardiovascular, and vocal fold engineering. In this study, we investigate an alternative cross-linking chemistry to form resilin-based protein hydrogels and demonstrate the versatility of the gels for investigating cell response to matrix stiffness. Specifically, transglutaminase was used to cross-link proteins and resulted in gel surfaces more suitable for long-term cell attachment compared to those formed by a Mannich-type condensation reaction...
August 8, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27341837/the-importance-of-stem-cell-engineering-in-head-and-neck-oncology
#5
REVIEW
Wojciech Barczak, Pawel Golusiński, Lukasz Luczewski, Wiktoria M Suchorska, Michal M Masternak, Wojciech Golusiński
Head and neck squamous cell carcinoma is the sixth leading cause of cancer worldwide. The most common risk factors are carcinogens (tobacco, alcohol), and infection of the human papilloma virus. Surgery is still considered as the treatment of choice in case of head and neck cancer, followed by a reconstructive surgery to enhance the quality of life in the patients. However, the widespread use of artificial implants does not provide appropriate physiological activities and often cannot act as a long-term solution for the patients...
October 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27316784/extracellular-matrix-for-vocal-fold-lamina-propria-replacement-a-review
#6
Emily A Wrona, Robert Peng, Milan R Amin, Ryan C Branski, Donald O Freytes
The vocal folds (VFs) are exposed to a number of injurious stimuli that frequently lead to aberrant structural alterations and altered biomechanical properties that clinically manifest as voice disorders. Therapies to restore both structure and function of this delicate tissue are ideal. However, such methods have not been adequately developed. Our group and others hypothesize that tissue engineering and regenerative medicine approaches, previously described for other tissue systems, hold significant promise for the VFs...
December 2016: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/27272832/magnetic-tissue-engineering-for-voice-rehabilitation-first-steps-in-a-promising-field
#7
Stephan Dürr, Christopher Bohr, Marina Pöttler, Stefan Lyer, Ralf Philipp Friedrich, Rainer Tietze, Michael Döllinger, Christoph Alexiou, Christina Janko
BACKGROUND/AIM: The voice is one of the most important instruments of communication between humans. It is the product of intact and well-working vocal folds. A defect of these structures causes dysphonia, associated with a clear reduction of quality of life. Tissue engineering of the vocal folds utilizing magnetic cell levitation after nanoparticle loading might be a technique to overcome this challenging problem. MATERIALS AND METHODS: Vocal fold fibroblasts (VFFs) were isolated from rabbit larynges and cultured...
June 2016: Anticancer Research
https://www.readbyqxmd.com/read/26956198/tissue-engineered-vocal-fold-mucosa-implantation-in-rabbits
#8
Travis L Shiba, Jordan Hardy, Georg Luegmair, Zhaoyan Zhang, Jennifer L Long
OBJECTIVE: To assess phonatory function and wound healing of a tissue-engineered vocal fold mucosa (TE-VFM) in rabbits. An "artificial" vocal fold would be valuable for reconstructing refractory scars and resection defects, particularly one that uses readily available autologous cells and scaffold. This work implants a candidate TE-VFM after resecting native epithelium and lamina propria in rabbits. STUDY DESIGN: Prospective animal study. SETTING: Research laboratory...
April 2016: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/26780495/elastic-proteins-and-elastomeric-protein-alloys
#9
REVIEW
Behnaz Aghaei-Ghareh-Bolagh, Suzanne M Mithieux, Anthony S Weiss
The elastomeric proteins elastin and resilin have been used extensively in the fabrication of biomaterials for tissue engineering applications due to their unique mechanical and biological properties. Tropoelastin is the soluble monomer component of elastin. Tropoelastin and resilin are both highly elastic with high resilience, substantial extensibility, high durability and low energy loss, which makes them excellent candidates for the fabrication of elastic tissues that demand regular and repetitive movement like the skin, lung, blood vessels, muscles and vocal folds...
June 2016: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/26774977/stem-cell-approaches-for-vocal-fold-regeneration
#10
Jonathan M Fishman, Jenny Long, Markus Gugatschka, Paolo De Coppi, Shigeru Hirano, Stellan Hertegard, Susan L Thibeault, Martin A Birchall
OBJECTIVES/HYPOTHESIS: Current interventions in the management of vocal fold (VF) dysfunction focus on conservative and surgical approaches. However, the complex structure and precise biomechanical properties of the human VF mean that these strategies have their limitations in clinical practice and in some cases offer inadequate levels of success. Regenerative medicine is an exciting development in this field and has the potential to further enhance VF recovery beyond conventional treatments...
August 2016: Laryngoscope
https://www.readbyqxmd.com/read/26632334/recombinant-resilin-based-bioelastomers-for-regenerative-medicine-applications
#11
Linqing Li, Atsushi Mahara, Zhixiang Tong, Eric A Levenson, Christopher L McGann, Xinqiao Jia, Tetsuji Yamaoka, Kristi L Kiick
The outstanding elasticity, excellent resilience at high-frequency, and hydrophilic capacity of natural resilin have motivated investigations of recombinant resilin-based biomaterials as a new class of bio-elastomers in the engineering of mechanically active tissues. Accordingly, here the comprehensive characterization of modular resilin-like polypeptide (RLP) hydrogels is presented and their suitability as a novel biomaterial for in vivo applications is introduced. Oscillatory rheology confirmed that a full suite of the RLPs can be rapidly cross-linked upon addition of the tris(hydroxymethyl phosphine) cross-linker, achieving similar in situ shear storage moduli (20 k ± 3...
January 21, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/26582902/bioengineered-vocal-fold-mucosa-for-voice-restoration
#12
Changying Ling, Qiyao Li, Matthew E Brown, Yo Kishimoto, Yutaka Toya, Erin E Devine, Kyeong-Ok Choi, Kohei Nishimoto, Ian G Norman, Tenzin Tsegyal, Jack J Jiang, William J Burlingham, Sundaram Gunasekaran, Lloyd M Smith, Brian L Frey, Nathan V Welham
Patients with voice impairment caused by advanced vocal fold (VF) fibrosis or tissue loss have few treatment options. A transplantable, bioengineered VF mucosa would address the individual and societal costs of voice-related communication loss. Such a tissue must be biomechanically capable of aerodynamic-to-acoustic energy transfer and high-frequency vibration and physiologically capable of maintaining a barrier against the airway lumen. We isolated primary human VF fibroblasts and epithelial cells and cocultured them under organotypic conditions...
November 18, 2015: Science Translational Medicine
https://www.readbyqxmd.com/read/24664167/fibroblast-like-cells-differentiated-from-adipose-derived-mesenchymal-stem-cells-for-vocal-fold-wound-healing
#13
Rong Hu, Wei Ling, Wen Xu, Demin Han
Tissue engineering has revealed the potential to regenerate injured vocal folds, and identification of the most suitable seed cells has remained a hot topic of research. The aim of this study was to implant fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells (ADSCs) in a canine acute vocal fold wound model. We then sought to characterize changes in the extracellular matrix (ECM) proteins of vocal fold lamina propria. For this purpose, ADSCs were induced to differentiate into fibroblasts under the regulation of connective tissue growth factor in vitro...
2014: PloS One
https://www.readbyqxmd.com/read/24403099/glottic-regeneration-with-a-tissue-engineering-technique-using-acellular-extracellular-matrix-scaffold-in-a-canine-model
#14
Morimasa Kitamura, Shigeru Hirano, Shin-Ichi Kanemaru, Yoshiharu Kitani, Satoshi Ohno, Tsuyoshi Kojima, Tatsuo Nakamura, Juichi Ito, Clark A Rosen, Thomas W Gilbert
Acellular extracellular matrix scaffold derived from porcine urinary bladder (UBM) is decellularized material that has shown success for constructive remodelling of various tissues and organs. The regenerative effects of UBM were reported for the tympanic membrane, oesophagus, trachea, larynx, pleura and pericardium in animal studies, with promising results. The aim of this study was to investigate the regenerative effects of UBM on hemilarynx, using a canine model. A left partial hemilaryngectomy was performed and the surgical defects were reconstructed by insertion of UBM scaffold...
October 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/23978518/the-use-of-laryngeal-mucosa-mesenchymal-stem-cells-for-the-repair-the-vocal-fold-injury
#15
Han Peng, Leiguo Ming, Runqin Yang, Yang Liu, Yuanyuan Liang, Yu Zhao, Yan Jin, Zhihong Deng
Stem cell transplantation is a kind of attractive and new approach that complements traditional restorative or surgical techniques for the regeneration of injured or pathologically damaged laryngeal tissues. However, the best cell delivery strategy remains to be identified. The objective of this study was to establish a new strategy to the healing of injured vocal fold, using laryngeal mucosa mesenchymal stem cells differentiating into myofibroblasts or fibroblasts and improving the reconstruction microenvironment in the vocal fold injury as a new alternative as seed cells for laryngeal tissue engineering...
December 2013: Biomaterials
https://www.readbyqxmd.com/read/23671810/current-applications-of-mesenchymal-stem-cells-for-tissue-replacement-in-otolaryngology-head-and-neck-surgery
#16
Suzanne N King, Summer E Hanson, Peiman Hematti, Susan L Thibeault
Cellular therapy utilizing adult mesenchymal stromal/stem cells (MSCs) may very well revolutionize the treatment of a variety of head and neck diseases through the restoration of normal structure and function. Transplanting allogeneic or autologous MSCs into damaged tissues can serve multiple regenerative functions through their self-renewal, differentiation capacity, immune modulation and secretion of bioactive molecules. Further, trophic factors expressed by MSCs have been shown to influence their microenvironment through the promotion of extracellular matrix remodeling, angiogenesis and wound healing needed to regenerate or replace injured tissues...
2012: American Journal of Stem Cells
https://www.readbyqxmd.com/read/23564555/in-vitro-characterization-of-macrophage-interaction-with-mesenchymal-stromal-cell-hyaluronan-hydrogel-constructs
#17
Suzanne N King, Summer E Hanson, Xia Chen, Jaehyup Kim, Peiman Hematti, Susan L Thibeault
Macrophages play a critical role in mediating not only normal tissue healing, but also the host reaction against biomaterial scaffolds. There is increasing interest in regenerative medicine to combine mesenchymal stromal/stem cells (MSCs) with biomaterial scaffolds to modulate inflammatory response while restoring tissue architecture. The objective of the current study was to investigate the interaction between MSCs (derived from bone marrow, adipose or vocal fold tissue) encapsulated in hyaluronan-based hydrogel and differentiating macrophages as measured by extracellular matrix (ECM) gene expression and cytokine, chemokine, and growth factor concentrations...
March 2014: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/22795981/regenerative-medicine-of-the-larynx-where-are-we-today-a-review
#18
REVIEW
Markus Gugatschka, Satoshi Ohno, Amulya Saxena, Shigeru Hirano
Tissue engineering is a multidimensional process combining cells, scaffold matrices, and chemical signals to produce a structure similar to a target tissue. These techniques have opened a completely new field in diagnosis and therapy in numerous fields, including that of laryngology. Laryngeal tissue engineering has emerged in the last decade, although clinical applications are rare. The reasons therefore are numerous including ethical reasons, as well as the extremely complex anatomical structure of the vocal fold...
September 2012: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/21689062/controlling-the-fibroblastic-differentiation-of-mesenchymal-stem-cells-via-the-combination-of-fibrous-scaffolds-and-connective-tissue-growth-factor
#19
Zhixiang Tong, Shilpa Sant, Ali Khademhosseini, Xinqiao Jia
Controlled differentiation of multi-potent mesenchymal stem cells (MSCs) into vocal fold-specific, fibroblast-like cells in vitro is an attractive strategy for vocal fold repair and regeneration. The goal of the current study was to define experimental parameters that can be used to control the initial fibroblastic differentiation of MSCs in vitro. To this end, connective tissue growth factor (CTGF) and micro-structured, fibrous scaffolds based on poly(glycerol sebacate) (PGS) and poly(ɛ-caprolactone) (PCL) were used to create a three-dimensional, connective tissue-like microenvironment...
November 2011: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/21391425/adipose-derived-mesenchymal-stem-cells-in-collagen-hyaluronic-acid-gel-composite-scaffolds-for-vocal-fold-regeneration
#20
Wen Xu, Rong Hu, Erzhong Fan, Demin Han
OBJECTIVES: We sought to characterize the changes in the extracellular matrix (ECM) of the lamina propria following the implantation of autologous adipose-derived mesenchymal stem cells (ADSCs) in composite scaffolds in a rabbit vocal fold wound model. METHODS: The ADSCs were co-cultured with collagen or hyaluronic acid gel. Each vocal fold was injured by localized resection and injected with ADSC complexes, ADSCs, or scaffolds or left untreated. From 15 days to 12 months, the vocal fold shape and the characteristics of the ECM components were analyzed...
February 2011: Annals of Otology, Rhinology, and Laryngology
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