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https://www.readbyqxmd.com/read/28715671/adipose-triglyceride-lipase-protein-abundance-and-translocation-to-the-lipid-droplet-increase-during-leptin-induced-lipolysis-in-bovine-adipocytes
#1
D A Koltes, M E Spurlock, D M Spurlock
Proper regulation of lipid metabolism is critical for preventing the development of metabolic diseases. It is clear that leptin plays a critical role in the regulation of energy homeostasis by regulating energy intake. However, leptin can also regulate energy homeostasis by inducing lipolysis in adipocytes, but it is unclear how the major lipases are involved in leptin-stimulated lipolysis. Therefore, the objectives of this study were to determine if (1) leptin acts directly to induce lipolysis in bovine adipocytes, (2) the potential lipases involved in leptin-induced lipolysis in bovine adipocytes, and (3) increases translocation of adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) during leptin-stimulated lipolysis in bovine stromal vascular cell-derived adipocytes...
June 8, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28713971/systematic-analysis-of-lncrnas-mirnas-and-mrnas-for-the-identification-of-biomarkers-for-osteoporosis-in-the-mandible-of-ovariectomized-mice
#2
Lingyu Hao, Jiayao Fu, Yawen Tian, Junhua Wu
Osteoporosis is a complex and multifactorial disease caused by an imbalance between bone formation and resorption. Post‑menopausal women with endogenous estrogen deficiency suffer from systemic bone loss and osteoporosis, and are at high risk of this affecting the jaw bones. MicroRNAs (miRNAs or miRs) have been implicated in the mechanisms of metabolic bone diseases and are expressed at differential levels in alveolar bone following ovariectomy. In the present study, we systematically analyzed the expression profiles of miRNAs, mRNAs and long non‑coding RNA (lncRNAs) in the mandible of ovariectomized (OVX) mice...
July 10, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28711458/the-collaborative-work-of-droplet-assembly
#3
REVIEW
Xiao Chen, Joel M Goodman
Three proteins have been implicated in the assembly of cytoplasmic lipid droplets: seipin, FIT2, and perilipin. This review examines the current theories of seipin function as well as the evidence for the involvement of all three proteins in droplet biogenesis, and ends with a proposal of how they collaborate to regulate the formation of droplets. This article is part of a Special Issue entitled: Recent Advances in Lipid Droplet Biology edited by Rosalind Coleman and Matthijs Hesselink.
July 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28703289/maresin-1-inhibits-tnf-alpha-induced-lipolysis-and-autophagy-in-3t3-l1-adipocytes
#4
Laura M Laiglesia, Silvia Lorente-Cebrián, Miguel López-Yoldi, Raquel Lanas, J Alfredo Martínez, Maria J Moreno-Aliaga
Obesity is associated with high pro-inflammatory cytokine levels like tumor necrosis factor-alpha (TNF-α), which promotes inflammation in adipose tissue. The omega-3 PUFAs, and their derived lipid mediators, such as Maresin 1 (MaR1) have anti-inflammatory effects on adipose tissue. This study aimed to analyze if MaR1 may counteract alterations induced by TNF-α on lipolysis and autophagy in mature 3T3-L1 adipocytes. Our data revealed that MaR1 (1-100 nM) inhibited the TNF-α-induced glycerol release after 48 h, which may be related to MaR1 ability of preventing the decrease of lipid droplet-coating protein perilipin and G0/G1 Switch 2 protein expression...
July 13, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28698229/changes-in-the-phosphoproteome-of-brown-adipose-tissue-during-hibernation-in-the-ground-squirrel-ictidomys-tridecemlineatus
#5
Didier Vertommen, Gaetan Herinckx, Nusrat Hussain, Fred Opperdoes, Kenneth B Storey, Mark H Rider
Mammalian hibernation is characterized by metabolic rate depression and a strong decrease in core body temperature. Brown adipose tissue (BAT), a thermogenic tissue that uses uncoupled mitochondrial respiration to generate heat instead of ATP, plays a major role in rewarming from deep torpor. In the present study we developed a label-free LC-MS strategy to investigate both differential protein expression and protein phosphorylation in BAT extracts from euthermic versus hibernating ground squirrels. In particular, we incorporated the filter-assisted sample preparation (FASP) protocol, which provides a more in-depth analysis compared with gel-based and other LC-MS proteomics approaches...
July 10, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28676863/muscle-lipid-metabolism-role-of-lipid-droplets-and-perilipins
#6
REVIEW
Pablo Esteban Morales, Jose Luis Bucarey, Alejandra Espinosa
Skeletal muscle is one of the main regulators of carbohydrate and lipid metabolism in our organism, and therefore, it is highly susceptible to changes in glucose and fatty acid (FA) availability. Skeletal muscle is an extremely complex tissue: its metabolic capacity depends on the type of fibers it is made up of and the level of stimulation it undergoes, such as acute or chronic contraction. Obesity is often associated with increased FA levels, which leads to the accumulation of toxic lipid intermediates, oxidative stress, and autophagy in skeletal fibers...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28659738/rosmarinic-acid-suppresses-adipogenesis-lipolysis-in-3t3-l1-adipocytes-lipopolysaccharide-stimulated-tumor-necrosis-factor-%C3%AE-secretion-in-macrophages-and-inflammatory-mediators-in-3t3-l1-adipocytes
#7
Yehua Rui, Lingxia Tong, Jinbo Cheng, Guiping Wang, Liqiang Qin, Zhongxiao Wan
Background: Rosmarinic acid (RA) is a natural phenol carboxylic acid with many promising biological effects. It may be a suitable candidate for improving obesity-related adipose tissue dysfunction. Objective: We aimed to investigate the therapeutic use of RA as an anti-obesity agent by measuring its effects on adipogenesis, lipolysis, and messenger RNA (mRNA) expression of major adipokines in 3T3-L1 adipocytes; and its effects on lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) secretion in macrophages and inflammatory mediators in 3T3-L1 adipocytes incubated with macrophage-conditioned medium (MCM)...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28627196/comparison-of-gene-expression-profile-between-blood-cells-and-white-adipose-tissue-of-patients-with-obesity
#8
Marcela Augusta de Souza Pinhel, Natália Yumi Noronha, Carolina Ferreira Nicoletti, Driele Cristina Gomes Quinhoneiro, Bruno Affonso Parenti Oliveira, Cristiana Cortes-Oliveira, Wilson Salgado-Junior, Wilson Araújo da Silva-Junior, Júlio Sergio Marchini, Dorotéia Rossi Silva Souza, Carla Barbosa Nonino
INTRODUCTION: Gene expression analyses from peripheral blood mononuclear cells (PBMC) and white adipose tissue are conflicting. It seems that results from single tissue are not enough to explain how changes affect humans as a complex biological system. OBJECTIVE: The aim of this study was to compare, from obesity subjects, PBMC and white adipose tissue gene expression that regulates adipogenesis (perilipin 1 [PLIN1], adrenoreceptor beta 3 [ADRB3] and peroxisome proliferator-activated receptor [PPARG2]) and the energy metabolism (uncoupling protein UCP1, UCP2 and UCP3) process...
June 5, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28617309/lipid-storage-and-autophagy-in-melanoma-cancer-cells
#9
Claudia Giampietri, Simonetta Petrungaro, Martina Cordella, Claudio Tabolacci, Luana Tomaipitinca, Antonio Facchiano, Adriana Eramo, Antonio Filippini, Francesco Facchiano, Elio Ziparo
Cancer stem cells (CSC) represent a key cellular subpopulation controlling biological features such as cancer progression in all cancer types. By using melanospheres established from human melanoma patients, we compared less differentiated melanosphere-derived CSC to differentiating melanosphere-derived cells. Increased lipid uptake was found in melanosphere-derived CSC vs. differentiating melanosphere-derived cells, paralleled by strong expression of lipogenic factors Sterol Regulatory Element-Binding Protein-1 (SREBP-1) and Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ)...
June 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28605462/increased-triacylglycerol-lipase-activity-in-adipose-tissue-of-lean-and-obese-men-during-endurance-exercise
#10
Anatoli Petridou, Athanasios Chatzinikolaou, Alexandra Avloniti, Athanasios Jamurtas, Gedeon Loules, Ioannis Papassotiriou, Ioannis Fatouros, Vassilis Mougios
Context: Although there is increasing information on the mechanism of lipolysis in adipose tissue, the effect of exercise on individual factors of lipolysis is less well understood. Objective: We aimed to compare changes in adipose tissue triacylglycerol lipase activity and gene expression of adipose triacylglycerol lipase (ATGL), hormone sensitive lipase (HSL), monoacylglycerol lipase (MGL), perilipin 1, and comparative gene identification 58 (CGI-58) during exercise between lean and obese men...
June 12, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28579235/haematopoietic-tlr4-deletion-attenuates-perivascular-brown-adipose-tissue-inflammation-in-atherosclerotic-mice
#11
Penghao Liu, Gaojian Huang, Zhiyong Cao, Qihai Xie, Tong Wei, Chenglin Huang, Qun Li, Mengwei Sun, Weili Shen, Pingjin Gao
AIMS: To investigate whether haematopoietic TLR4 deletion attenuates perivascular brown adipose tissue inflammation in atherosclerotic mice. METHODS AND RESULTS: Experiments were performed using irradiated LDL receptor-deficient (LDLR(-/-)) mice with marrow from either TLR4-deficient (TLR4(-/-)) or age-matched wild-type (WT) mice. After 12 weeks of being fed a high-cholesterol diet, TLR4(-/-)→LDLR(-/-) mice developed fewer atherosclerotic lesions in the aorta compared to WT→LDLR(-/-) mice...
June 1, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28560826/training-alters-the-distribution-of-perilipin-proteins-in-muscle-following-acute-free-fatty-acid-exposure
#12
S O Shepherd, J A Strauss, Q Wang, J J Dube, B Goodpaster, D G Mashek, L S Chow
Because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyceride (IMTG) storage, we investigated the hypothesis that differential protein content of PLINs and their distribution with LDs may be linked to the diverse lipid storage in muscle between trained and sedentary individuals. Trained (n = 11) and sedentary (n = 10) subjects, matched for age, sex and BMI, received either a 6 h lipid or glycerol infusion in the setting of a concurrent hyperinsulinaemic-euglycaemic clamp...
May 31, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28548876/the-constitutive-lipid-droplet-protein-plin2-regulates-autophagy-in-liver
#13
Tsung-Huang Tsai, Elaine Chen, Lan Li, Pradip Saha, Hsiao-Ju Lee, Li-Shin Huang, Gregory S Shelness, Lawrence Chan, Benny Hung-Junn Chang
Excess triglyceride (TG) accumulation in the liver underlies fatty liver disease, a highly prevalent ailment. TG occurs in the liver sequestered in lipid droplets, the major lipid storage organelle. Lipid droplets are home to the lipid droplet proteins, the most abundant of which are the perilipins (PLINs), encoded by 5 different genes, Plin1 to Plin5. Of the corresponding gene products, PLIN2 is the only constitutive and ubiquitously expressed lipid droplet protein that has been used as a protein marker for lipid droplets...
May 26, 2017: Autophagy
https://www.readbyqxmd.com/read/28523759/role-of-sulfotransferases-in-resveratrol-metabolism-in-human-adipocytes
#14
Nele Gheldof, Sofia Moco, Christian Chabert, Tony Teav, Denis Barron, Jorg Hager
SCOPE: Polyphenols such as resveratrol received interest for their wide-ranging biological benefits, including anti-obesity potential, mimicking effects of caloric restriction with reduced body fat and increased energy expenditure. However, resveratrol is rapidly metabolized, and it is not completely understood which form of resveratrol is responsible for the effects observed within target cells such as adipocytes. Also the role of metabolizing enzymes has not been investigated before...
May 18, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28501397/effect-of-conjugated-linoleic-acid-and-acetate-on-milk-fat-synthesis-and-adipose-lipogenesis-in-lactating-dairy-cows
#15
N Urrutia, K J Harvatine
During biohydrogenation-induced milk fat depression (MFD), nutrients are spared from milk fat synthesis and are available for other metabolic uses. Acetate is the major carbon source spared and it may increase lipid synthesis in adipose tissue during MFD. The objective of this study was to compare the effect of trans-10,cis-12 conjugated linoleic acid (CLA) and the amount of acetate spared during CLA-induced MFD on adipose tissue lipogenesis. Nine multiparous, lactating, ruminally cannulated Holstein cows (244 ± 107 d in milk; 25 ± 8...
July 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28486770/melatonin-promotes-survival-of-non-vascularized-fat-grafts-and-enhances-the-viability-and-migration-of-human-adipose-derived-stem-cells-via-down-regulation-of-acute-inflammatory-cytokines
#16
Shaun S Tan, Weiqing Zhan, Christopher J Phoon, Xiaolian Han, Diego Marre, Sholeh Boodhun, Jason A Palmer, Geraldine M Mitchell, Wayne A Morrison
Non-vascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content to lipoaspirate. We have recently shown that the use of melatonin, a ROS scavenger, protects human ASCs from hydrogen peroxide induced oxidative stress and cell death in vitro but its role as a pharmacologic adjunct in clinical fat grafting has not been studied...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28459610/3-3-diindolylmethane-suppresses-adipogenesis-using-ampk%C3%AE-dependent-mechanism-in-3t3-l1-adipocytes-and-caenorhabditis-elegans
#17
Jihye Lee, Yiren Yue, Yeonhwa Park, Seong-Ho Lee
3,3'-diindolylmethane is a major in vivo metabolite of indole-3-carbinol, a bioactive compound found in cruciferous vegetables. Although 3,3'-diindolylmethane has been implicated to possess antitumorigenic and anti-inflammatory properties, the effect of 3,3'-diindolylmethane on adipogenesis has not been explored previously. Thus, the present study was conducted to determine if 3,3'-diindolylmethane affects adipogenesis using 3T3-L1 adipocytes and Caenorhabditis elegans. Treatment of 3,3'-diindolylmethane significantly reduced fat accumulation without affecting viability in 3T3-L1 adipocytes...
July 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28456765/the-skeletal-and-heart-muscle-triacylglycerol-lipolysis-revisited
#18
M Knapp, J Gorski
For 40 years, the enzyme hormone sensitive lipase was considered to hydrolyze the first ester bond of the triacylglycerol moiety and thus initiate hydrolysis. However, 12 years ago a new lipolytic enzyme, termed adipose triglyceride lipase was discovered. It was further shown that the process of lipolysis of triacylglycerol to diacylglycerol and fatty acid is initiated by adipose triglyceride lipase and not by hormone sensitive lipase, responsible for hydrolysis of diacylglycerol to monoacyglycerol and fatty acid...
February 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28454542/perilipins-a-diversity-of-intracellular-lipid-droplet-proteins
#19
REVIEW
Hiroyuki Itabe, Tomohiro Yamaguchi, Satomi Nimura, Naoko Sasabe
Intracellular lipid droplets (LDs) are found in a wide variety of cell types and have been recognized as organelles with unique spherical structures. Although LDs are not stable lipid-depots, they are active sites of neutral lipid metabolism, and comprise neutral lipid or cholesterol cores surrounded by phospholipid monolayers containing specialized proteins. However, sizes and protein compositions vary between cell and tissue types. Proteins of the perilipin family have been associated with surfaces of LDs and all carry a conserved 11-mer repeat motif...
April 28, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28438719/chick-subcutaneous-and-abdominal-adipose-tissue-depots-respond-differently-in-lipolytic-and-adipogenic-activity-to-%C3%AE-melanocyte-stimulating-hormone-%C3%AE-msh
#20
Steven L Shipp, Guoqing Wang, Mark A Cline, Elizabeth R Gilbert
In birds, α-MSH is anorexigenic, but effects on adipose tissue are unknown. Four day-old chicks were intraperitoneally injected with 0 (vehicle), 5, 10, or 50μg of α-MSH and subcutaneous and abdominal adipose tissue collected at 60min for RNA isolation (n=10). Plasma was collected post-euthanasia at 60 and 180min for measuring non-esterified fatty acids (NEFA) and α-MSH (n=10). Relative to the vehicle, food intake was reduced in the 50μg-treated group. Plasma NEFAs were greater in 10μg than vehicle-treated chicks at 3h...
July 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
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