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Perilipin

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https://www.readbyqxmd.com/read/28429532/neu1-sialidase-interacts-with-perilipin-1-on-lipid-droplets-and-inhibits-lipolysis-in-3t3-l1-adipocytes
#1
Yujin Natori, Miwako Nasui, Fumiko Kihara-Negishi
Fatty acids are stored within adipocytes in lipid droplets (LDs) as triacylglycerol (TG), which is converted to free fatty acid (FFA) and glycerol via lipolysis. Increased plasma FFA levels in obesity are associated with several clinical conditions. We previously found that Neu1 activity is aberrant in the epididymal fat and liver of obese and diabetic mice. Here, we examined involvement of Neu1 in lipolysis in 3T3-L1 adipocytes. Small interfering RNA against Neu1 was introduced into adipocytes, and glycerol concentrations were measured in the culture medium...
April 21, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28427098/opa1-in-lipid-metabolism-function-of-opa1-in-lipolysis-and-thermogenesis-of-adipocytes
#2
Dinh-Toi Chu, Yang Tao, Kjetil Taskén
OPA1 (Optic Atrophy 1) is a mitochondrial GTPase known to regulate fission of mitochondria. It was recently also shown to locate on lipid droplets in adipocytes where it functions as an A-kinase anchoring protein (AKAP) that mediates adrenergic control of lipolysis by facilitating PKA phosphorylation of perilipin (Plin1). In brown adipocytes indirect evidence support the notion that OPA1 regulation of fission serves to increase thermogenesis, which thereby contributes to dissipation of energy. In white adipocytes, OPA1 located on lipid droplets serves as a gatekeeper to control lipolysis induced by adrenergic agonists...
April 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28398656/a-biomimetic-hydrogel-functionalized-with-adipose-ecm-components-as-a-microenvironment-for-the-3d-culture-of-human-and-murine-adipocytes
#3
Fiona Louis, Pauline Pannetier, Zied Souguir, Didier Le Cerf, Philippe Valet, Jean-Pierre Vannier, Guillaume Vidal, Elise Demange
The lack of relevant in vitro models for adipose tissue makes necessary the development of a more physiological environment providing spatial and chemical cues for the effective maturation of adipocytes. We developed a biofunctionalized hydrogel with components of adipose extracellular matrix: collagen I, collagen VI and the cell binding domain of fibronectin and we compared it to usual 2D cultures on plastic plates. This scaffold allowed 3D culture of mature adipocytes from the preadipocytes cell lines 3T3-L1 and 3T3-F442A, as well as primary Human White Preadipocytes (HWP), acquiring in vivo-like organization, with spheroid shaped adipocytes forming multicellular aggregates...
April 11, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28348166/mfn2-is-critical-for-brown-adipose-tissue-thermogenic-function
#4
Marie Boutant, Sameer S Kulkarni, Magali Joffraud, Joanna Ratajczak, Miriam Valera-Alberni, Roy Combe, Antonio Zorzano, Carles Cantó
Mitochondrial fusion and fission events, collectively known as mitochondrial dynamics, act as quality control mechanisms to ensure mitochondrial function and fine-tune cellular bioenergetics. Defective mitofusin 2 (Mfn2) expression and enhanced mitochondrial fission in skeletal muscle are hallmarks of insulin-resistant states. Interestingly, Mfn2 is highly expressed in brown adipose tissue (BAT), yet its role remains unexplored. Using adipose-specific Mfn2 knockout (Mfn2-adKO) mice, we demonstrate that Mfn2, but not Mfn1, deficiency in BAT leads to a profound BAT dysfunction, associated with impaired respiratory capacity and a blunted response to adrenergic stimuli...
March 27, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28337143/anti-adipogenic-effects-and-mechanisms-of-ginsenoside-rg3-in-pre-adipocytes-and-obese-mice
#5
Longyun Zhang, Lijuan Zhang, Xiaoyong Wang, Hongwei Si
Red or black ginseng has been reported more powerful than white/fresh ginseng in dealing with various diseases/conditions including obesity. The major reason is that heating/steaming, the process of making red or black ginseng, produces large amount of bioactive compounds including ginsenoside Rg3 (Rg3), which are trace in fresh or white ginseng. In the present study, Rg3 was applied both in pre-adipocytes and obese mice to investigate the anti-adipogenic effects and relevant mechanisms. Our results show that Rg3 dose-dependently inhibited cell differentiation both in 3T3-L1 cells (30, 50, and 100 μM) and human primary pre-adipocytes (10, 20, and 30 μM)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28292967/pik3r1-is-required-for-glucocorticoid-induced-perilipin-1-phosphorylation-in-lipid-droplet-for-adipocyte-lipolysis
#6
Taiyi Kuo, Tzu-Chieh Chen, Rebecca A Lee, Nguyen Huynh Thao Nguyen, Augusta E Broughton, Danyun Zhang, Jen-Chywan Wang
Glucocorticoids promote lipolysis in white adipose tissue (WAT) to adapt to energy demands under stress, while superfluous lipolysis causes metabolic disorders, including dyslipidemia and hepatic steatosis. Glucocorticoid-induced lipolysis requires the phosphorylation of cytosolic hormone sensitive lipase (HSL) and perilipin 1 (Plin1) in the lipid droplet by protein kinase A (PKA). We previously identified Pik3r1 (a.k.a. p85α) as a glucocorticoid receptor target gene. Here, we found that glucocorticoids increased HSL phosphorylation, but not Plin1 phosphorylation, in adipose tissue-specific Pik3r1-null (AKO) mice...
March 14, 2017: Diabetes
https://www.readbyqxmd.com/read/28291828/retinoic-acid-exacerbates-chlorpyrifos-action-in-ensuing-adipogenic-differentiation-of-c3h10t%C3%A2-cells-in-a-gsk3%C3%AE-dependent-pathway
#7
Harkirat Singh Sandhu, A J S Bhanwer, Sanjeev Puri
The cell differentiation can be exploited as a paradigm to evaluate the effects of noxious chemicals, on human health, either alone or in combinations. In this regard, the effect of a known cell differentiation agent, retinoic acid (RA) was analyzed in the presence of a noxious chemical chlorpyrifos (CPF), an organophosphate (OP), the receptors of which have recently been localized to mesenchymal stem cells (MSCs). The observed imbalance of adipogenic to skeletal differentiation by CPF together with conundrum about adipogenic potential of RA prompted us to delineate their combinatorial effects on C3H10T½MSC-like undifferentiated cells...
2017: PloS One
https://www.readbyqxmd.com/read/28284697/mir-27a-controls-triacylglycerol-synthesis-in-bovine-mammary-epithelial-cells-by-targeting-peroxisome-proliferator-activated-receptor-gamma
#8
K Q Tang, Y N Wang, L S Zan, W C Yang
Growing evidence has revealed that microRNA are central elements in milk fat synthesis in mammary epithelial cells. A negative regulator of adipocyte fat synthesis, miR-27a has been reported to be involved in the regulation of milk fat synthesis in goat mammary epithelial cells; however, the regulatory role of miR-27a in bovine milk fat synthesis remains unclear. In the present study, primary bovine mammary epithelial cells (BMEC) were harvested from mid-lactation cows and cultured in Dulbecco's modified Eagle's medium/F-12 medium with 10% fetal bovine serum, 5 μg/mL of insulin, 1 μg/mL of hydrocortisone, 2 μg/mL of prolactin, 1 μg/mL of progesterone, 100 U/mL of penicillin, and 100 μg/mL of streptomycin...
May 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28279068/%C3%AE-catenin-mediates-anti-adipogenic-and-anticancer-effects-of-arctigenin-in-preadipocytes-and-breast-cancer-cells
#9
Jihye Lee, Jee-Young Imm, Seong-Ho Lee
Arctigenin is a lignan abundant in Asteraceae plants and has anti-inflammatory, antiobesity, and anticancer activities. Obesity is one of the leading causes of several types of cancers including breast cancer. The current study was performed to investigate if arctigenin suppresses differentiation of preadipocytes and proliferation of breast cancer cells and to explore potential molecular mechanisms. Treatment of arctigenin reduced lipid accumulation in differentiated 3T3-L1 adipocytes in a dose- and time-dependent manner without toxicity...
March 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28274232/perilipin-polymorphisms-are-risk-factors-for-the-development-of-obesity-in-adolescents-a-case-control-study
#10
Yavuz Tokgöz, Ishak Abdurrahman Işık, Soheil Akbari, Tuncay Kume, Oya Sayın, Esra Erdal, Nur Arslan
BACKGROUND: The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at the PLIN locus in adolescents with obesity and their connection with serum adipokines. METHODS: A total of 308 children (206 obese, 66.8% and 102 healthy control, 33.2%) between the ages of 10-18 years were included into the study. PLIN gene analysis [PLIN 1, PLIN 4, PLIN 6, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T] were studied by Real Time-PCR...
March 9, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28260008/tauroursodeoxycholic-acid-inhibits-tnf-%C3%AE-induced-lipolysis-in-3t3-l1-adipocytes-via-the-ire-jnk-perilipin-a-signaling-pathway
#11
Wenyan Xia, Yu Zhou, Lijing Wang, Linxi Wang, Xiaoying Liu, Yichuan Lin, Qing Zhou, Jianqing Huang, Libin Liu
The present study investigated the effects of tauroursodeoxycholic acid (TUDCA) on the lipolytic action of tumor necrosis factor (TNF)-α in 3T3-L1 adipocytes. Following treatment with TNF‑α, cell viability was determined by MTT assay to select the optimum concentration and duration of TNF‑α treatment in 3T3‑L1 adipocytes. Intracellular lipid droplet dispersion and glycerin content in culture media were determined to evaluate the effect of TUDCA on TNF‑α‑induced lipolysis in 3T3‑L1 adipocytes...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28244868/lipid-droplet-biology-and-evolution-illuminated-by-the-characterization-of-a-novel-perilipin-in-teleost-fish
#12
James G Granneman, Vickie A Kimler, Huamei Zhang, Xiangqun Ye, Xixia Luo, John H Postlethwait, Ryan Thummel
Perilipin (PLIN) proteins constitute an ancient family important in lipid droplet (LD) formation and triglyceride metabolism. We identified an additional PLIN clade (plin6) that is unique to teleosts and can be traced to the two whole genome duplications that occurred early in vertebrate evolution. Plin6 is highly expressed in skin xanthophores, which mediate red/yellow pigmentation and trafficking, but not in tissues associated with lipid metabolism. Biochemical and immunochemical analyses demonstrate that zebrafish Plin6 protein targets the surface of pigment-containing carotenoid droplets (CD)...
February 28, 2017: ELife
https://www.readbyqxmd.com/read/28218306/plin5-alleviates-myocardial-ischaemia-reperfusion-injury-by-reducing-oxidative-stress-through-inhibiting-the-lipolysis-of-lipid-droplets
#13
Pengfei Zheng, Zhonglin Xie, Yuan Yuan, Wen Sui, Chao Wang, Xing Gao, Yuanlin Zhao, Feng Zhang, Yu Gu, Peizhen Hu, Jing Ye, Xuyang Feng, Lijun Zhang
Myocardial ischaemia-reperfusion (I/R) injury is a complex pathophysiological process. Current research has suggested that energy metabolism disorders, of which the abnormal consumption of fatty acids is closely related, compose the main pathological basis for myocardial I/R injury. Lipid droplets (LD) are critical regulators of lipid metabolism by LD-associated proteins. Among the lipid droplet proteins, the perilipin family members regulate lipolysis and lipogenesis through different mechanisms. Plin5, an important perilipin protein, promotes LD generation and lowers fatty acid oxidation, thus protecting the myocardium from lipotoxicity...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28217782/receptor-for-hyaluronan-mediated-motility-rhamm-hmmr-is-a-novel-target-for-promoting-subcutaneous-adipogenesis
#14
S B Bahrami, C Tolg, T Peart, C Symonette, M Veiseh, J U Umoh, D W Holdsworth, J B McCarthy, L G Luyt, M J Bissell, A Yazdani, E A Turley
Hyaluronan, CD44 and the Receptor for Hyaluronan-Mediated Motility (RHAMM, gene name HMMR) regulate stem cell differentiation including mesenchymal progenitor differentiation. Here, we show that CD44 expression is required for subcutaneous adipogenesis, whereas RHAMM expression suppresses this process. We designed RHAMM function blocking peptides to promote subcutaneous adipogenesis as a clinical and tissue engineering tool. Adipogenic RHAMM peptides were identified by screening for their ability to promote adipogenesis in culture assays using rat bone marrow mesenchymal stem cells, mouse pre-adipocyte cell lines and primary human subcutaneous pre-adipocytes...
March 1, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28211464/molecular-basis-of-abhd5-lipolysis-activation
#15
Matthew A Sanders, Huamei Zhang, Ljiljana Mladenovic, Yan Yuan Tseng, James G Granneman
Alpha-beta hydrolase domain-containing 5 (ABHD5), the defective gene in human Chanarin-Dorfman syndrome, is a highly conserved regulator of adipose triglyceride lipase (ATGL)-mediated lipolysis that plays important roles in metabolism, tumor progression, viral replication, and skin barrier formation. The structural determinants of ABHD5 lipolysis activation, however, are unknown. We performed comparative evolutionary analysis and structural modeling of ABHD5 and ABHD4, a functionally distinct paralog that diverged from ABHD5 ~500 million years ago, to identify determinants of ABHD5 lipolysis activation...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28173833/human-breast-adipose-tissue-characterization-of-factors-that-change-during-tumor-progression-in-human-breast-cancer
#16
Sabrina Johanna Fletcher, Paula Alejandra Sacca, Mercedes Pistone-Creydt, Federico Andrés Coló, María Florencia Serra, Flavia Eliana Santino, Corina Verónica Sasso, Constanza Matilde Lopez-Fontana, Rubén Walter Carón, Juan Carlos Calvo, Virginia Pistone-Creydt
BACKGROUND: Adipose microenvironment is involved in signaling pathways that influence breast cancer. We aim to characterize factors that are modified: 1) in tumor and non tumor human breast epithelial cell lines when incubated with conditioned media (CMs) from human breast cancer adipose tissue explants (hATT) or normal breast adipose tissue explants (hATN); 2) in hATN-CMs vs hATT-CMs; 3) in the tumor associated adipocytes vs. non tumor associated adipocytes. METHODS: We used hATN or hATT- CMs on tumor and non-tumor breast cancer cell lines...
February 7, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28124996/expression-of-lipid-metabolism-related-proteins-differs-between-invasive-lobular-carcinoma-and-invasive-ductal-carcinoma
#17
Yoon Jin Cha, Hye Min Kim, Ja Seung Koo
We comparatively investigated the expression and clinical implications of lipid metabolism-related proteins in invasive lobular carcinoma (ILC) and invasive ductal carcinoma (IDC) of the breast. A total of 584 breast cancers (108 ILC and 476 IDC) were subjected to tissue microarray and immunohistochemical analysis for lipid metabolism-related proteins including hormone-sensitive lipase (HSL), perilipin A, fatty acid binding protein (FABP)4, carnitine palmitoyltransferase (CPT)-1, acyl-CoA oxidase 1, and fatty acid synthetase (FASN)...
January 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28123934/circulating-fgf21-in-humans-is-potently-induced-by-short-term-overfeeding-of-carbohydrates
#18
Anne-Marie Lundsgaard, Andreas M Fritzen, Kim A Sjøberg, Lene S Myrmel, Lise Madsen, Jørgen F P Wojtaszewski, Erik A Richter, Bente Kiens
OBJECTIVE: Fibroblast-growth factor 21 (FGF21) is thought to be important in metabolic regulation. Recently, low protein diets have been shown to increase circulating FGF21 levels. However, when energy contribution from dietary protein is lowered, other macronutrients, such as carbohydrates, must be increased to meet eucaloric balance. This raises the possibility that intake of a diet rich in carbohydrates may induce an increase in plasma FGF21 levels per se. Here we studied the role of dietary carbohydrates on the levels of circulating FGF21 and concomitant physiologic effects by feeding healthy men a carbohydrate rich diet without reducing protein intake...
January 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28102311/plin2-is-a-key-regulator-of-the-unfolded-protein-response-and-endoplasmic-reticulum-stress-resolution-in-pancreatic-%C3%AE-cells
#19
Elaine Chen, Tsung Huang Tsai, Lan Li, Pradip Saha, Lawrence Chan, Benny Hung-Junn Chang
Progressive pancreatic β cell failure underlies the transition of impaired glucose tolerance to overt diabetes; endoplasmic reticulum (ER) stress expedites β cell failure in this situation. ER stress can be elicited by lipotoxicity and an increased demand for insulin in diabetes. We previously reported that the lipid droplet protein perilipin 2 (PLIN2) modulates lipid homeostasis in the liver. Here, we show that PLIN2 modulates the unfolded protein response (UPR) and ER stress in pancreatic β cells. PLIN2 expression goes up when β cells are exposed to a lipid load or to chemical ER stress inducers...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28096119/commentary-on-exposure-to-tumescent-solution-significantly-increases-phosphorylation-of-perilipin-in-adipocytes
#20
Ali Mojallal, Christo Shipkov
No abstract text is available yet for this article.
February 2017: Aesthetic Surgery Journal
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