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Indira T Kudva, Michelle Q Carter, Vijay K Sharma, Judith A Stasko, Jorge A Giron
: Our recent studies have shown that Intimin and the Locus of Enterocyte Effacement-encoded proteins do not play a role in Escherichia coli O157:H7 (O157) adherence to the bovine recto-anal junction squamous epithelial (RSE) cells. To define factors that play a contributory role, we investigated the role of curli, a fimbrial adhesin commonly implicated in adherence to various fomites, plant, and human epithelial cells, in O157 adherence to RSE cells. Specifically, we examined (i) wild-type strains of O157; (ii) curli variants of O157 strains; (iii) isogenic curli-deletion mutants of O157; and (iv) adherence-inhibition of O157 using anti-curlin sera...
October 14, 2016: Applied and Environmental Microbiology
Jara E Jonker, Eryn T Liem, Nynke J Elzenga, Bouwe Molenbuur, Monika Trzpis, Paul M A Broens
OBJECTIVE: To determine the prevalence of congenital heart defects (CHDs) in patients with mild or severe congenital anorectal malformations (CARMs), and whether all patients with CARM need pediatric cardiology screening. STUDY DESIGN: We included 129 patients with CARM born between 2004 and 2013, and referred to University Medical Center Groningen. Recto-perineal and recto-vestibular fistulas were classified as mild CARMs, all others as severe. Significant patent foramen ovale, secundum atrial septal defect, and small ventricular septum defect were classified as minor CHDs, all others as major...
September 26, 2016: Journal of Pediatrics
Giuseppe Palermo, Angelo Totaro, Emilio Sacco, Nazario Foschi, Gaetano Gulino, Marco Racioppi, Pierfrancesco Bassi, Francesco Pinto
BACKGROUND: Prostate cancer (PCa) is the most commonly diagnosed malignancy in men and the second leading cause of cancer death in developed countries. Despite the primary treatments, 20-30% of patients experience a recurrence. The main objective of this study was to evaluate the clinical efficacy of salvage HIFU after radical prostatectomy in terms of biochemical free survival rate (BFSR) and PSA nadir. METHODS: Twenty two patients with local recurrence of Pca after radical prostatectomy underwent HIFU as first-line salvage therapy...
September 28, 2016: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
Giancarlo D'Ambrosio, Gianfrancesco Intini, Andrea Balla, Silvia Quaresima, Andrea Picchetto, Ardit Seitaj, Salvatore Campo, Francesca De Laurentis, Alessandro M Paganin
AIM: Recto-Urinary Fistula (RUF) is a rare complication of pelvic surgery. Different approaches are reported in literature but a gold standard treatment has not yet been achieved. Transanal Endoscopic Microsurgery (TEM) is a miniinvasive approach with well known advantages as magnification, 3D view and lighting of the operative field. Aim of the present review is to report the current evidence in literature about technique and results of RUF treatment by TEM and to suggest some key points for its correct management...
2016: Annali Italiani di Chirurgia
F Shakir, H Jan, C Pearson, P Haines, W Rae-Mitchell, A Kent
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
A Racca, E Biscaldi, V Remorgida, U Leone Roberti Maggiore, V G Vellone, P L Venturini, S Ferrero
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
A Racca, V G Vellone, G Camerini, U Leone Roberti Maggiore, F Sozzi, V Remorgida, P L Venturini, E Biscaldi, S Ferrero
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
A Nargund, R Penketh, A Griffiths, E Bruen, A Roberts, L Kwasnicka
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
A Lam, T A Almotrafi, M Mangot
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
Laura Lombardi, Elena Garrisi, Matteo Ricco', Federico Marchesi, Emilio Casolari, Claudia Gatti, Carmine G Del Rossi
PURPOSE: We aimed at investigating bowel function in children and adolescents with anorectal malformations (ARMs) and assess their quality of life (QoL), in order to define a personal program of bowel management improving both clinical condition and self-confidence. METHODS: A total of 55 patients treated for ARM by Posterior Sagittal Ano-Recto-Plasty (PSARP) from 2000 to 2014 were included into the study. The parents of 41 patients answered two telephone questionnaires about the parents' self-efficacy and about QoL (GIQLI)...
2016: Acta Bio-medica: Atenei Parmensis
Giuseppe Giuliani, Francesco Guerra, Diego Coletta, Marco La Torre, Giorgio Franco, Costantino Leonardo, Aldo Infantino, Filippo La Torre
AIM: Rectourethral fistula (RUF) is a rare complication of radical prostatectomy. We report a trans-perineal approach using a fibrin sealant haemostatic patch. METHOD: Five consecutive patients who developed RUF following radical prostatectomy had a direct transperineal repair with a haemostatic patch (TachoSil(®) ) and were assessed at a median follow up of 35.5 (21-45) months. RESULTS: There were no early postoperative complications. The average length of hospital stay was 5 (4-7) days...
September 15, 2016: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
Najib Aziz, Roger Detels, L Cindy Chang, Anthony W Butch
OBJECTIVE: Uncontrolled HIV infection progresses to the depletion of systemic and mucosal CD4 and AIDS. Early HIV infection may be associated with increases in the concentration of MIP-3α in the blood and gut fluids. MIP-3α/CCL20 is the only chemokine known to interact with CCR6 receptors which are expressed on immature dendritic cells and both effector and memory CD8(+) and CD4(+) T cells. The role and prognostic value of blood levels of MIP-3α in HIV-infected individuals has yet to be described...
July 2016: Journal of AIDS & Clinical Research
Anna C Seale, Angela C Koech, Anna E Sheppard, Hellen C Barsosio, Joyce Langat, Emily Anyango, Stella Mwakio, Salim Mwarumba, Susan C Morpeth, Kirimi Anampiu, Alison Vaughan, Adam Giess, Polycarp Mogeni, Leahbell Walusuna, Hope Mwangudzah, Doris Mwanzui, Mariam Salim, Bryn Kemp, Caroline Jones, Neema Mturi, Benjamin Tsofa, Edward Mumbo, David Mulewa, Victor Bandika, Musimbi Soita, Maureen Owiti, Norris Onzere, A Sarah Walker, Stephanie J Schrag, Stephen H Kennedy, Greg Fegan, Derrick W Crook, James A Berkley
Streptococcus agalactiae (group B streptococcus, GBS) causes neonatal disease and stillbirth, but its burden in sub-Saharan Africa is uncertain. We assessed maternal recto-vaginal GBS colonization (7,967 women), stillbirth and neonatal disease. Whole-genome sequencing was used to determine serotypes, sequence types and phylogeny. We found low maternal GBS colonization prevalence (934/7,967, 12%), but comparatively high incidence of GBS-associated stillbirth and early onset neonatal disease (EOD) in hospital (0...
2016: Nature Microbiology
Stefano Cosma, Matteo Salgarello, Marcello Ceccaroni, Giancarlo Gorgoni, Francesca Riboni, Ernesto La Paglia, Saverio Danese, Chiara Benedetto
AIM: Preoperative workup of deep infiltrating endometriosis is limited in the evaluation of extragenital and extrapelvic disease and in distinguishing between the previous surgical scar and active lesion. Histological verification remains the gold standard for diagnosis. The aim of this study was therefore to evaluate positron emission tomography-computed tomography (PET/CT) with an experimental estrogen receptor tracer (16α-[18F]fluoro-17β-estradiol; [18F]FES) for accurate staging and non-invasive diagnosis of the disease...
August 24, 2016: Journal of Obstetrics and Gynaecology Research
Melissa J Firth, Katrina J Sharples, Victoria A Hinder, Jerome Macapagal, Diana Sarfati, Sarah L Derrett, Andrew G Hill, Charis Brown, Papaarangi Mj Reid, Ross A Lawrenson, Carol Atmore, John P Keating, Mark Jeffery, Adrian H Secker, Charles De Groot, Christopher Gca Jackson, Michael Pn Findlay
AIM: Colorectal cancer is one of the most common cancers, and second-leading cause of cancer-related death, in New Zealand. The PIPER (Presentations, Investigations, Pathways, Evaluation, Rx [treatment]) project was undertaken to compare presentation, investigations, management and outcomes by rurality, ethnicity and deprivation. This paper reports the methods of the project, a comparison of PIPER patient diagnoses to the New Zealand Cancer Registry (NZCR) data, and the characteristics of the PIPER cohort...
2016: New Zealand Medical Journal
Christine C Akoh, Eva K Pressman, Elizabeth Cooper, Ruth Anne Queenan, Julie Pillittere, Kimberly O O'Brien
STUDY OBJECTIVE: Our objective was to identify risk factors associated with maternal infections and placental inflammation in pregnant adolescents attending an urban adolescent maternity clinic. DESIGN: This cross-sectional, descriptive study used survey and medical chart data collected at entry and prospectively across gestation. The prevalence of maternal infections and placental inflammation was determined and potential risk factors were identified. SETTING: Rochester Adolescent Maternal Program (RAMP) in Rochester, NY...
August 10, 2016: Journal of Pediatric and Adolescent Gynecology
Philip H Pucher, Damian Mayo, Anthony R Dixon, Andrew Clarke, Michael J Lamparelli
BACKGROUND: Laparoscopic ventral mesh rectopexy (VMR) is an effective and well-recognised treatment for symptoms of obstructive defecation in the context of rectal prolapse and recto-rectal intussusception. However, due to the technical complexity of VMR, a significant learning curve has been previously described. This paper examines the effect of proctored adoption of VMR on learning curves, operative times, and outcomes. METHODS: A retrospective database analysis of two district general hospitals was conducted, with inclusion of all cases performed by two surgeons since first adoption of the procedure in 2007-2015...
August 5, 2016: Surgical Endoscopy
Ching-Heng Yen, Chin-Yu Liu, Tai-Lung Cha, Sheng-Tang Wu, En Meng, Guang-Huan Sun, Dah-Shyong Yu, Hong-I Chen, Sun-Yran Chang, Chih-Wei Tsao
INTRODUCTION: Emphysematous epididymo-orchitis is a rare cause of acute scrotum pain characterized by gas formation within the tissue. Diabetes mellitus and recto-seminal fistula secondary to sigmoid diverticulitis are generally accepted as being responsible for this disease. However, prostate invasion secondary to rectal cancer may be considered to be a newly identified pathogenetic mechanism. Herein, we report this rare case and illustrate the pathogenesis. CASE PRESENTATION: A 69-year-old man arrived at our emergency department presenting with sepsis and acute scrotal pain...
July 2016: Medicine (Baltimore)
Erik N Mayer, Jonathan D Tward, Mitchell Bassett, Sara M Lenherr, James M Hotaling, William O Brant, William T Lowrance, Jeremy B Myers
OBJECTIVE: To describe the management of grade 4 Radiation Therapy Oncology Group (RTOG) urinary adverse events (UAEs) after radiotherapy (RT) for prostate cancer (PCa). We hypothesized grade 4 UAEs often require complex surgical management and subject patients to significant morbidity. METHODS: A single-center retrospective review, over a 6-year period (2010-2015), identified men with RTOG grade 4 UAEs after RT for PCa. RT was classified as combined modality therapy (radical prostatectomy (RP) followed by external beam radiotherapy (EBRT), EBRT + low-dose brachytherapy (LDR), EBRT + high-dose brachytherapy (HDR), or other combinations of RT) or single modality RT...
July 28, 2016: BJU International
Shawqi Arafat, Mhd Belal Alsabek, Faisal Almousa, Muhammad Assem Kubtan
INTODUCTION: Endometriosis is a disease in which endometrial epithelium implanted outside the uterus. Although the endometrial tissue can implant anywhere, the most common places are the ovary and pelvic peritoneum. We present a rare case of recto-sigmoid endometriosis that causes a complete large bowel obstruction in a non-reproductive age woman who came with no specific symptoms of endometriosis and the diagnosis was made after surgical resection. CASE PRESENTATION: A 50 years old female who never been married and admitted to have no sexual experience in her life, presented with symptoms of acute bowel obstruction...
2016: International Journal of Surgery Case Reports
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