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https://www.readbyqxmd.com/read/28330277/classical-and-bayesian-predictions-applied-to-bacillus-toxin-production
#1
Karim Ennouri, Rayda Ben Ayed, Maura Mazzarello, Ennio Ottaviani, Fathi Hertelli, Hichem Azzouz
Bacillus thuringiensis is a bacterium with unusual properties that make it useful for pest control in ecoagriculture. It can form a parasporal crystal containing polypeptides (also called delta-endotoxins). These entomopathogenic toxins are made during the stationary phase of the bacterial growth cycle and were initially characterized as an insect pathogen. Nowadays, the use of saturated two-level designs is very popular. This method is especially used in industrial applications where the cost of experiments is expensive...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330182/efficient-single-step-chromatographic-purification-of-recombinant-human-antithrombin-rhat-from-saccharomyces-cerevisiae
#2
Maheswara Reddy Mallu, Sandeep Vemula, Srinivasa Reddy Ronda
Antithrombin (AT) is a glycoprotein that inactivates the several physiological target enzymes of coagulation system. The effect of purification strategies plays a crucial role in getting maximum recovery of yield, purity and biological activity of recombinant human antithrombin (rhAT). In the present work, the task of purifying rhAT from Saccharomyces cerevisiae BY4741 has been carried out using two different approaches such as cross flow filtration (CFF) system and chromatography methods. In the first approach, the protein was concentrated and partially purified through CFF to achieve maximum recovery yield and purity of 87 and 94 %, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330142/an-overview-of-physico-chemical-mechanisms-of-biogas-production-by-microbial-communities-a-step-towards-sustainable-waste-management
#3
REVIEW
Ramansu Goswami, Pritam Chattopadhyay, Arunima Shome, Sambhu Nath Banerjee, Amit Kumar Chakraborty, Anil K Mathew, Shibani Chaudhury
Biogas is a combination of methane, CO2, nitrogen, H2S and traces of few other gases. Almost any organic waste can be biologically transformed into biogas and other energy-rich organic compounds through the process of anaerobic digestion (AD) and thus helping in sustainable waste management. Although microbes are involved in each step of AD, knowledge about those microbial consortia is limited due to the lack of phylogenetic and metabolic data of predominantly unculturable microorganisms. However, culture-independent methods like PCR-based ribotyping has been successfully employed to get information about the microbial consortia involved in AD...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330102/biodegradation-of-flubendiamide-by-a-newly-isolated-chryseobacterium-sp-strain-ssj1
#4
Shrinivas S Jadhav, M David
Flubendiamide, as a new class (Phthalic acid diamide) of pesticide with a wide spectrum of activity against lepidopteran pests extensively used alone or in combination with other insecticides in agriculture system to get protection from insect pests. Due to high specificity and limited approach towards non-target organism, the extensive use of this pesticide as an alternate for organophosphate and organochlorine pesticides, causing an eventual increase in environmental pollution. Five flubendiamide-resistant bacterial strains were isolated during the present study from agriculture soil considering previous history of pesticide application...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#5
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329691/myocardial-infarction-primes-autoreactive-t-cells-through-activation-of-dendritic-cells
#6
Katrien Van der Borght, Charlotte L Scott, Veronika Nindl, Ann Bouché, Liesbet Martens, Dorine Sichien, Justine Van Moorleghem, Manon Vanheerswynghels, Sofie De Prijck, Yvan Saeys, Burkhard Ludewig, Thierry Gillebert, Martin Guilliams, Peter Carmeliet, Bart N Lambrecht
Peripheral tolerance is crucial for avoiding activation of self-reactive T cells to tissue-restricted antigens. Sterile tissue injury can break peripheral tolerance, but it is unclear how autoreactive T cells get activated in response to self. An example of a sterile injury is myocardial infarction (MI). We hypothesized that tissue necrosis is an activator of dendritic cells (DCs), which control tolerance to self-antigens. DC subsets of a murine healthy heart consisted of IRF8-dependent conventional (c)DC1, IRF4-dependent cDC2, and monocyte-derived DCs...
March 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/28328586/percutaneous-isolated-hepatic-perfusion-hemodynamic-monitoring-and-goal-directed-anesthetic-management-a-case-report
#7
Oscar Martin-Delgado, Miguel Rodriguez, Andres Lopez, Miguel A Reina, Emilio De Vicente, Yolanda Quijano, Antonio Echenagusia, Juan A Martinez
The percutaneous isolated hepatic perfusion utilizes a venovenous bypass to administer high-dose chemotherapy exclusively in the liver, getting depurated through a hemofilter before returning to the systemic circulation. The hepatic perfusion is managed under general anesthesia and invasive monitoring as a result of very abrupt changes in venous return and vascular resistances because of the isolation of the hepatic territory and absorption of circulating catecholamines by the hemofilter. We report a case in which we describe the technique, physiologic implications, anesthetic, and goal-directed hemodynamic management for this procedure...
March 15, 2017: A & A Case Reports
https://www.readbyqxmd.com/read/28328576/chronic-pain-patients-perspectives-of-medical-cannabis
#8
Brian J Piper, Monica L Beals, Alexander T Abess, Stephanie D Nichols, Maurice Martin, Catherine M Cobb, Rebecca M DeKeuster
Medical cannabis (MC) is employed for a variety of conditions including chronic pain. The goal of this report was to provide an in-depth qualitative exploration of patient perspectives on the strengths and limitations of MC. Members of MC dispensaries (N = 984) in New England including two-thirds with a history of chronic pain completed an online survey. In response to "How effective is medical cannabis in treating your symptoms or conditions?", with options of 0% "no relief" to 100% "complete relief", the average was 74...
March 21, 2017: Pain
https://www.readbyqxmd.com/read/28328490/the-coming-gray-tide-wanted-health-innovations-for-an-increasingly-older-population
#9
Leslie Mertz
The human population is getting older, and technology will play a key role in addressing the pressures this aging will place on healthcare systems. According to the 2015 United Nations' World Population Ageing report [1], the number of people worldwide 60 and older will increase from one in eight in 2015 to one in six by 2030 and to one in five by 2050; in Europe and "Northern America" (mainly the United States and Canada), those 60 and older will make up 25% of the population by 2030, and in Asia and Latin America, the number is predicted to be 17%...
March 2017: IEEE Pulse
https://www.readbyqxmd.com/read/28328308/sexual-and-gender-minority-breast-cancer-patients-choosing-bilateral-mastectomy-without-reconstruction-i-feel-like-i-now-have-a-body-that-fits-me
#10
Maria Teresa Brown, Jane A McElroy
Sexual and gender minority (SGM) breast cancer patients have begun embracing the choice to "go flat" or opt out of reconstruction after bilateral mastectomy, though little is known about this population. SGM breast cancer survivors were identified through purposive and referral sampling and invited to participate in a web-based survey containing both closed- and open-ended items. Of the 68 SGM breast cancer survivors aged 18-75 years who completed the survey between May 2015 and January 2016, 25% reported "going flat" (flattoppers)...
March 22, 2017: Women & Health
https://www.readbyqxmd.com/read/28328232/learning-by-doing-the-md-pa-interprofessional-education-rural-rotation
#11
Ryan T Palmer, Curt Stilp
While much investment has gone into developing interprofessional education (IPE) curriculum for healthcare professional students, many of these efforts have focused on classroom rather than clinical environments. Implementing robust IPE experiences into clinical training is often complicated by obstacles such as differing rotating schedules and differing curricular requirements. The Combined Medical-Physician Assistant Student Rural Rotation (Med-PARR) at the Oregon Health and Science University takes a practical approach to these challenges...
January 2017: Rural and Remote Health
https://www.readbyqxmd.com/read/28328128/first-drug-to-treat-spinal-muscular-atrophy-gets-fda-approval-costly-injectable-shown-to-stall-debilitating-muscle-wasting-effects-of-rare-genetic-disorder
#12
(no author information available yet)
No abstract text is available yet for this article.
April 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28328071/investigation-of-c3-s-hydration-mechanism-by-transmission-electron-microscope-tem-with-integrated-super-x-tm-eds-system
#13
Y Sakalli, R Trettin
Tricalciumsilicate (C3 S, Alite) is the major component of the Portland cement clinker. Hydration of Alite is decisive in influencing the properties of the resulting material. This is due to its high content in cement. The mechanism of the hydration of C3 S is very complicated and not yet fully understood. There are different models describing the hydration of C3 S in various ways. In this work for a better understanding of hydration mechanism, the hydrated C3 S was investigated by using the transmission electron microscope (TEM) and for the first time, the samples for the investigations were prepared by using of focused ion beam from sintered pellets of C3 S...
March 22, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28327351/parkinson-s-patients-can-rely-on-perspective-cues-to-perceive-3d-space
#14
Alexandra Séverac Cauquil, Fabienne Ory-Magne, Vincent Jardiné, Monique Galitzky, Maxime Rosito, Christine Brefel-Courbon, Simona Celebrini
3D perception, which is necessary for an optimal navigation in our environment, relies on 2 complementary kinds of cues; binocular cues allowing precise depth localization near the point of visual interest and monocular ones that are necessary for correct global perception of visual space. Recent studies described deficient binocular 3D vision in PD patients; here we tested 3D vision in PD patients when based on monocular cues (m3D). Sixteen PD patients and 16 controls had to categorize visual stimuli as perceived in 2D (flat) or 3D (with depth)...
March 18, 2017: Brain Research
https://www.readbyqxmd.com/read/28327231/attractiveness-biases-are-the-tip-of-the-iceberg-in-biological-markets
#15
Pat Barclay
Physical attractiveness affects how one gets treated, but it is just a single component of one's overall "market value." One's treatment depends on other markers of market value, including social status, competence, warmth, and any other cues of one's ability or willingness to confer benefits on partners. To completely understand biased treatment, we must also incorporate these other factors.
January 2017: Behavioral and Brain Sciences
https://www.readbyqxmd.com/read/28326747/laboratory-methods-for-diagnostics-of-hiv-infection-and-hiv-associated-neuroinfections
#16
(no author information available yet)
HIV Infection resulting in AIDS remains serious global public health problem. In the fight with the global health problem plays a key role a simple, reliable and fast diagnostics. An important method in diagnostics is the identification and detection of viral capside p24 antigen levels. Fourth generation tests for the diagnostics of HIV infection simultaneously detect the presence of HIV antibodies and p24 antigen. Based on the monitoring of CD4 count, we can estimate the stage in which the infection is, and we can also suggest a therapeutic approach...
January 22, 2017: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28326742/comparative-assessment-of-calcitonin-stimulation-test-using-calcium-gluconate-and-pentagastrin-and-the-usefulness-of-procalcitonin-basic-and-post-stimulation-concentrations-in-the-diagnosis-of-patients-after-surgery-for-medullary-thyroid-cancer
#17
(no author information available yet)
OBJECTIVES: The aim of the study was to compare the calcitonin (CT) stimulation tests with tests of calcium gluconate (CaG) and pentagastrin (PG), their tolerance and usefulness of PCT in the patients' diagnosis with active Medullary thyroid cancer (MCT) after thyroidectomy. METHODS: CT was marked in serum by the immunosorbent sandwich test. PCT was marked by the immunosorbent sandwich test, with the final reading of fluorenscence. PG was given intravenously at a dose of 0...
December 22, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28326354/vitamin-d-status-in-children-over-three-decades-do-children-get-enough-vitamin-d
#18
Björn Andersson, Diana Swolin-Eide, Per Magnusson, Kerstin Albertsson-Wikland
Vitamin D is a key player in the endocrine regulation of calcium and phosphate metabolism and plays a pivotal role in the acquisition of bone mass during childhood. This study investigated long-term data of vitamin D levels in children and adolescents between 1 and 18 years of age. Serum 25-hydroxyvitamin D (25(OH)D) was analyzed between 1982 and 2013 in 2048 Swedish Caucasian children (mean age ± SD, 8.59 ± 3.68 years; 1197 boys). Overall, 704 (34%) children had below recommended levels of 50 nmol/L; however, only 63 (3%) had levels below 25 nmol/L, i...
December 2016: Bone Reports
https://www.readbyqxmd.com/read/28326318/could-early-surgery-get-beneficial-in-adult-patients-with-active-native-infective-endocarditis-a-meta-analysis
#19
REVIEW
Liqun Jia, Zanxin Wang, Qiang Fu, Huaien Bu, Minxin Wei
After a thorough search through the database as PubMed database and Embase database, the clinical experimental articles have been selected out on the effects of early surgery on the treatment of active native infective endocarditis. The quality of the trials included in this study was assessed by researcher according to the Cochrane Handbook for Systematic Reviews of Interventions, version 5.1.0. A meta-analysis was carried out in terms of clinical efficacy criteria by RevMan 5.3 software. Based on the results, we cautiously conclude that early surgery used for active native infective endocarditis could reduce in-hospital mortality, follow-up mortality, and IE-related mortality...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28326011/the-virtual-trial
#20
Willem de Haan
Although brain network analysis in neurodegenerative disease is still a fairly young discipline, expectations are high. The robust theoretical basis, the straightforward detection and explanation of otherwise intangible complex system phenomena, and the correlations of network features with pathology and cognitive status are qualities that show the potential power of this new instrument. We expect "connectomics" to eventually better explain and predict that essential but still poorly understood aspect of dementia: the relation between pathology and cognitive symptoms...
2017: Frontiers in Neuroscience
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