keyword
https://read.qxmd.com/read/37373279/morphological-characterization-of-human-lung-cancer-organoids-cultured-in-type-i-collagen-hydrogels-a-histological-approach
#21
JOURNAL ARTICLE
Irene Monleón-Guinot, Lara Milian, Patricia Martínez-Vallejo, María Sancho-Tello, Mauro Llop-Miguel, José Marcelo Galbis, Antonio Cremades, Carmen Carda, Manuel Mata
The malignity of lung cancer is conditioned by the tumor microenvironment (TME), in which cancer-associated fibroblasts (CAFs) are relevant. In this work, we generated organoids by combining A549 cells with CAFs and normal fibroblasts (NF) isolated from adenocarcinoma tumors. We optimized the conditions for their manufacture in a short time. We evaluated the morphology of organoids using confocal microscopy analysis of F-actin, vimentin and pankeratin. We determined the ultrastructure of the cells in the organoids via transmission electron microscopy and the expression of CDH1, CDH2 and VIM via RT-PCR...
June 14, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37370129/panhematopoietic-rna-barcoding-enables-kinetic-measurements-of-nucleate-and-anucleate-lineages-and-the-activation-of-myeloid-clones-following-acute-platelet-depletion
#22
JOURNAL ARTICLE
Edyta E Wojtowicz, Jayna J Mistry, Vladimir Uzun, Charlotte Hellmich, Anita Scoones, Desmond W Chin, Laura M Kettyle, Francesca Grasso, Allegra M Lord, David J Wright, Graham J Etherington, Petter S Woll, Mirjam E Belderbos, Kristian M Bowles, Claus Nerlov, Wilfried Haerty, Leonid V Bystrykh, Sten Eirik W Jacobsen, Stuart A Rushworth, Iain C Macaulay
BACKGROUND: Platelets and erythrocytes constitute over 95% of all hematopoietic stem cell output. However, the clonal dynamics of HSC contribution to these lineages remains largely unexplored. RESULTS: We use lentiviral genetic labeling of mouse hematopoietic stem cells to quantify output from all lineages, nucleate, and anucleate, simultaneously linking these with stem and progenitor cell transcriptomic phenotypes using single-cell RNA-sequencing. We observe dynamic shifts of clonal behaviors through time in same-animal peripheral blood and demonstrate that acute platelet depletion shifts the output of multipotent hematopoietic stem cells to the exclusive production of platelets...
June 27, 2023: Genome Biology
https://read.qxmd.com/read/37275615/apparent-total-tract-digestibility-and-palatability-of-extruded-diets-with-graded-levels-of-whole-soybeans-by-dogs
#23
JOURNAL ARTICLE
Hee S Kim, Sang Li, Yi Zheng, Charles G Aldrich
Fat has high energy density and is considered one of the primary energy sources for dogs, however, increasing fat level in dry dog food has been challenging due to the lubrication and limitation of the coating system. The objective was to determine the effect of whole soybeans (WSB) on nutrient digestibility, stool quality, and palatability by dogs. The corn gluten meal, chicken fat, and brewers rice were replaced by WSB at 10, 20, and 30% (WSB10, WSB20, and WSB30, respectively) in the base diet (WSB0). Twelve beagles were randomly assigned...
2023: Frontiers in Veterinary Science
https://read.qxmd.com/read/37257384/sorption-affinity-and-mechanisms-of-per-and-polyfluoroalkyl-substances-pfass-with-commercial-sorbents-implications-for-passive-sampling
#24
JOURNAL ARTICLE
Sara Ghorbani Gorji, Darryl W Hawker, Rachel Mackie, Christopher P Higgins, Karl Bowles, Yan Li, Sarit Kaserzon
Effective monitoring tools, including passive samplers, are essential for the wide range of per- and polyfluoroalkyl substances (PFASs) in aquatic matrices. However, knowledge of the extent and mechanisms of PFASs sorption with sorbents in a passive sampling context is limited. To address this, sorption behavior of 45 anionic, neutral and zwitterionic PFASs ranging in perfluorocarbon chain length (C3 -C16 ) and functional groups with 11 different commercial sorbents (cross-linked β-cyclodextrin polymers, activated carbon, anion exchange (AE), cation exchange, hydrophilic-lipophilic balanced (HLB) and non-polar) was investigated...
May 24, 2023: Journal of Hazardous Materials
https://read.qxmd.com/read/37245150/multiplex-gene-editing-to-promote-cell-survival-using-low-ph-clustered-regularly-interspaced-short-palindromic-repeats-activation-crispra-gene-perturbation
#25
JOURNAL ARTICLE
Hunter Levis, Jacob Weston, Brooke Austin, Bryce Larsen, Matthew Ginley-Hidinger, Sarah E Gullbrand, Brandon Lawrence, Robby D Bowles
BACKGROUND AIMS: Lower back pain is the leading cause of disability worldwide and is often linked to degenerative disc disease (DDD), the breakdown of intervertebral discs. The majority of treatment options for DDD are palliative, with clinicians prescribing medication or physical therapy to return the patient to work. Cell therapies are promising treatment options with the potential to restore functional physiological tissue and treat the underlying causes of DDD. DDD is characterized by biochemical changes in the microenvironment of the disc, including changes in nutrient levels, hypoxia, and changes in pH...
May 27, 2023: Cytotherapy
https://read.qxmd.com/read/37005503/first-report-of-heterodera-zeae-koshy-swarup-sethi-1971-corn-cyst-nematode-infecting-corn-zea-mays-in-spain
#26
JOURNAL ARTICLE
Juan Emilio Palomares-Rius, Ilenia Clavero-Camacho, Carolina Cantalapiedra-Navarrete, Luis F Roca-Castillo, Antonio Archidona-Yuste, Pablo Castillo
Heterodera zeae Koshy, Swarup & Sethi, 1971 (corn cyst nematode) is an important disease of corn in several areas of the world, including India, Nepal, Pakistan, Egypt, USA, Greece and Portugal (Subbotin et al., 2010). It is a sedentary semi-endoparasite feeding on corn roots and other Poaceae plants and has been associated with significant yield losses in corn (Subbotin et al., 2010). During autumn 2022 a plant-parasitic nematode survey performed in corn at central-western area of Spain (Talavera de la Reina, Toledo), revealed a commercial field with stunted plants...
April 2, 2023: Plant Disease
https://read.qxmd.com/read/36986995/reproductive-ecology-of-the-invasive-alien-shrub-pyracantha-angustifolia-in-the-grassland-biome-south-africa
#27
JOURNAL ARTICLE
Lehlohonolo D Adams, Dino Giovannoni, Vincent R Clark, Sandy-Lynn Steenhuisen, Grant D Martin
Knowledge on reproductive traits of problematic invasive alien plants, such as the woody invasive shrub Pyracantha angustifolia of temperate Chinese origin, can help better manage invasive species. To determine factors contributing to its invasion, we investigated floral visitors and pollen loads, self-compatibility, seed set, seed rain, soil seed banks, and seed longevity in the soil. Generalist insects were recorded visiting flowers and all carried pollen loads of high purity (>70%). Floral visitor exclusion experiments showed that P...
March 14, 2023: Plants (Basel, Switzerland)
https://read.qxmd.com/read/36655352/fuligopyrones-from-the-fruiting-bodies-of-myxomycete-fuligo-septica-offer-short-term-protection-from-abiotic-stress-induced-by-uv-radiation
#28
JOURNAL ARTICLE
Scott A Minns, Simon Bowles, Ernest Lacey, John A Kalaitzis, Daniel Vuong, Mark S Butler, Andrew M Piggott
The myxomycete Fuligo septica , colloquially referred to as "dog vomit fungus", forms vibrant yellow fruiting bodies (aethalia) on wood chips during warm and humid conditions in spring. In 2018, ideal climatic conditions in Sydney, Australia, provided a rare opportunity to access abundant quantities of F. septica aethalia, which enabled the isolation, purification, structure elucidation, and biological screening of two avenalumamide pyrones, fuligopyrone ( 1 ) and fuligopyrone B ( 2 ). While 1 and 2 did not exhibit any appreciable biological activity, their significant UV absorption at 325 nm suggested they may be acting as transient sunscreens to help protect the fruiting mass from exposure to sunlight...
January 18, 2023: Journal of Natural Products
https://read.qxmd.com/read/36631318/evaporative-water-loss-from-dairy-cows-in-climate-controlled-respiration-chambers
#29
JOURNAL ARTICLE
M Zhou, P W G Groot Koerkamp, T T T Huynh, A J A Aarnink
The effects of ambient temperature (AT) on total evaporative water loss from dairy cows at different relative humidity (RH) and air velocity (AV) levels were studied. Twenty Holstein dairy cows with an average parity of 2.0 ± 0.7 and body weight of 687 ± 46 kg participated in the study. Two climate-controlled respiration chambers were used. The experimental indoor climate was programmed to follow a diurnal pattern with AT at night being 9°C lower than during the day. Night AT was gradually increased from 7 to 21°C and day AT was increased from 16°C to 30°C within an 8-d period, both with an incremental change of 2°C/d...
January 9, 2023: Journal of Dairy Science
https://read.qxmd.com/read/36576914/coming-together-in-a-digital-age-community-twitter-responses-in-the-wake-of-a-campus-shooting
#30
JOURNAL ARTICLE
Jessamyn Bowling, Erika Montanaro, Sarai Guerrero Ordonez, Sean McCabe, Shayna Farris, Neielle Saint-Cyr, Wade Glaser, Robert J Cramer, Jennifer Langhinrichsen-Rohling, Annelise Mennicke
Campus mass shootings have become a pressing policy and public health matter. Twitter is a platform used for processing events among interested community members. Examining the responses of invested community members to a mass shooting on a college campus provides evidence for how this type of violence affects the immediate community and the larger public. These responses may reflect either content (e.g. context-specific) or emotions (e.g. humor). Aims Using Twitter data, we analyzed the emotional responses as well as the nature of non-affective short-term reactions, in response to the April 2019 shooting at UNC Charlotte...
2022: PloS One
https://read.qxmd.com/read/36530067/diet-therapy-using-a-small-rice-bowl-among-japanese-men-with-diabetes-a-randomized-controlled-trial
#31
JOURNAL ARTICLE
Misa Shimpo, Shiori Toga-Sato, Takahiro Tosaki
BACKGROUND: This study aimed to investigate the effectiveness at 1 month and 3 months of using a smaller rice bowl for diet therapy among Japanese men with type 2 diabetes. METHODS: A parallel-group randomized controlled trial was conducted at a medical clinic in Japan. The participants were men with type 2 diabetes mellitus, aged 20-80 years, with glycosylated hemoglobin <8.5%, and who ate rice one or more times per day at home. The intervention group (36 men) received a small rice bowl from which to eat the usual diet therapy, and the control group (38 men) received only the usual diet therapy...
December 19, 2022: Journal of obesity & metabolic syndrome
https://read.qxmd.com/read/36409647/the-effects-of-bowling-lines-and-lengths-on-the-spatial-distribution-of-successful-power-hitting-strokes-in-international-men-s-one-day-and-t20-cricket
#32
JOURNAL ARTICLE
Mikael Jamil, Samuel Kerruish, Marco Beato, Stuart A McErlain-Naylor
This study examined 503 power-hitting strokes that resulted in the maximum of 6-runs being scored in international men's one-day and T20 cricket. Chi-Squared analyses were conducted to determine if performance and situational variables were associated with the distribution (direction) of aerial power-hitting strokes. Results revealed that bowling length, bowling line, bowler type and powerplays were all significantly ( p < 0.001) associated with ball-hitting distribution. Post-hoc analysis of the standardised residuals revealed that greater than expected 6's were scored behind square and were associated with short-pitched bowling, fast bowling and the power-play...
November 21, 2022: Journal of Sports Sciences
https://read.qxmd.com/read/36404265/daily-cannabis-use-is-associated-with-sleep-duration-differentially-across-ages
#33
JOURNAL ARTICLE
Joshua E Gonzalez, Steven A Shea, Nicole P Bowles
OBJECTIVE: To assess the relationship between frequency of cannabis use and sleep duration across age in a large US population (235,667 people). METHODS: Multinomial logistic regression was used to evaluate the association between the frequency of cannabis use and sleep duration using cross sectional data from the 2016-2018 Behavioral Risk Factor Surveillance System. RESULTS: When adjusted for sociodemographic factors, health related variables, and stratified by age we found that young adults (18-44 years) who reported daily-use (≥16 uses a month) had an increased risk ratio (RR [95% CI]) for either short or long sleep (1...
November 17, 2022: Sleep Health
https://read.qxmd.com/read/36383648/the-winchcombe-meteorite-a-unique-and-pristine-witness-from-the-outer-solar-system
#34
JOURNAL ARTICLE
Ashley J King, Luke Daly, James Rowe, Katherine H Joy, Richard C Greenwood, Hadrien A R Devillepoix, Martin D Suttle, Queenie H S Chan, Sara S Russell, Helena C Bates, James F J Bryson, Patricia L Clay, Denis Vida, Martin R Lee, Áine O'Brien, Lydia J Hallis, Natasha R Stephen, Romain Tartèse, Eleanor K Sansom, Martin C Towner, Martin Cupak, Patrick M Shober, Phil A Bland, Ross Findlay, Ian A Franchi, Alexander B Verchovsky, Feargus A J Abernethy, Monica M Grady, Cameron J Floyd, Matthias Van Ginneken, John Bridges, Leon J Hicks, Rhian H Jones, Jennifer T Mitchell, Matthew J Genge, Laura Jenkins, Pierre-Etienne Martin, Mark A Sephton, Jonathan S Watson, Tobias Salge, Katherine A Shirley, Rowan J Curtis, Tristram J Warren, Neil E Bowles, Finlay M Stuart, Luigia Di Nicola, Domokos Györe, Adrian J Boyce, Kathryn M M Shaw, Tim Elliott, Robert C J Steele, Pavel Povinec, Matthias Laubenstein, David Sanderson, Alan Cresswell, Anthony J T Jull, Ivan Sýkora, Sanjana Sridhar, Richard J Harrison, Francesca M Willcocks, Catherine S Harrison, Daniel Hallatt, Penny J Wozniakiewicz, Mark J Burchell, Luke S Alesbrook, Aishling Dignam, Natasha V Almeida, Caroline L Smith, Brett Clark, Emma R Humphreys-Williams, Paul F Schofield, Luke T Cornwell, Vassilia Spathis, Geraint H Morgan, Mark J Perkins, Richard Kacerek, Peter Campbell-Burns, Francois Colas, Brigitte Zanda, Pierre Vernazza, Sylvain Bouley, Simon Jeanne, Mike Hankey, Gareth S Collins, John S Young, Clive Shaw, Jana Horak, Dave Jones, Nick James, Steve Bosley, Alan Shuttleworth, Paul Dickinson, Ian McMullan, Derek Robson, Andrew R D Smedley, Ben Stanley, Richard Bassom, Mark McIntyre, Adam A Suttle, Richard Fleet, Luc Bastiaens, Míra B Ihász, Sarah McMullan, Sarah J Boazman, Zach I Dickeson, Peter M Grindrod, Annemarie E Pickersgill, Colin J Weir, Fiona M Suttle, Sarah Farrelly, Ieun Spencer, Sheeraz Naqvi, Ben Mayne, Dan Skilton, Dan Kirk, Ann Mounsey, Sally E Mounsey, Sarah Mounsey, Pamela Godfrey, Lachlan Bond, Victoria Bond, Cathryn Wilcock, Hannah Wilcock, Rob Wilcock
Direct links between carbonaceous chondrites and their parent bodies in the solar system are rare. The Winchcombe meteorite is the most accurately recorded carbonaceous chondrite fall. Its pre-atmospheric orbit and cosmic-ray exposure age confirm that it arrived on Earth shortly after ejection from a primitive asteroid. Recovered only hours after falling, the composition of the Winchcombe meteorite is largely unmodified by the terrestrial environment. It contains abundant hydrated silicates formed during fluid-rock reactions, and carbon- and nitrogen-bearing organic matter including soluble protein amino acids...
November 18, 2022: Science Advances
https://read.qxmd.com/read/36339261/retrosnake-a-modular-pipeline-to-detect-human-endogenous-retroviruses-in-genome-sequencing-data
#35
JOURNAL ARTICLE
Renata Kabiljo, Harry Bowles, Heather Marriott, Ashley R Jones, Clement R Bouton, Richard J B Dobson, John P Quinn, Ahmad Al Khleifat, Chad M Swanson, Ammar Al-Chalabi, Alfredo Iacoangeli
Human endogenous retroviruses (HERVs) integrated into the human genome as a result of ancient exogenous infections and currently comprise ∼8% of our genome. The members of the most recently acquired HERV family, HERV-Ks, still retain the potential to produce viral molecules and have been linked to a wide range of diseases including cancer and neurodegeneration. Although a range of tools for HERV detection in NGS data exist, most of them lack wet lab validation and they do not cover all steps of the analysis...
November 18, 2022: IScience
https://read.qxmd.com/read/36336356/looking-on-the-horizon-potential-and-unique-approaches-to-developing-radiation-countermeasures-for-deep-space-travel
#36
JOURNAL ARTICLE
Rihana S Bokhari, Afshin Beheshti, Sarah E Blutt, Dawn E Bowles, David Brenner, Robert Britton, Lawrence Bronk, Xu Cao, Anushree Chatterjee, Delisa E Clay, Colleen Courtney, Donald T Fox, M Waleed Gaber, Sharon Gerecht, Peter Grabham, David Grosshans, Fada Guan, Erin A Jezuit, David G Kirsch, Zhandong Liu, Mirjana Maletic-Savatic, Kyle M Miller, Ruth A Montague, Prashant Nagpal, Sivan Osenberg, Luke Parkitny, Niles A Pierce, Christopher Porada, Susan M Rosenberg, Paul Sargunas, Sadhana Sharma, Jamie Spangler, Daniel Naveed Tavakol, Dilip Thomas, Gordana Vunjak-Novakovic, Chunbo Wang, Luke Whitcomb, Damian W Young, Dorit Donoviel
Future lunar missions and beyond will require new and innovative approaches to radiation countermeasures. The Translational Research Institute for Space Health (TRISH) is focused on identifying and supporting unique approaches to reduce risks to human health and performance on future missions beyond low Earth orbit. This paper will describe three funded and complementary avenues for reducing the risk to humans from radiation exposure experienced in deep space. The first focus is on identifying new therapeutic targets to reduce the damaging effects of radiation by focusing on high throughput genetic screens in accessible, sometimes called lower, organism models...
November 2022: Life Sciences in Space Research
https://read.qxmd.com/read/36263340/what-is-a-better-value-for-your-time-anterior-cervical-discectomy-and-fusion-versus-cervical-disc-arthroplasty
#37
JOURNAL ARTICLE
Austen David Katz, Junho Song, Daniel Bowles, Terence Ng, Eric Neufeld, Sayyida Hasan, Dean Perfetti, Nipun Sodhi, David Essig, Jeff Silber, Sohrab Virk
Introduction: Compared to anterior cervical discectomy and fusion (ACDF), the motion preservation of cervical disc arthroplasty (CDA) provides an attractive alternative with similar short-term results. However, there is a paucity of the economics of performing CDA over ACDF. Study Design: This was retrospective study. Objective: The objective of this study is to evaluate relative-value-units (RVUs), operative time, and RVUs-per-minute between single-level ACDF and CDA...
July 2022: Journal of Craniovertebral Junction and Spine
https://read.qxmd.com/read/36256029/three-dimensional-stitching-of-stereo-deflectometry-based-on-marker-points
#38
JOURNAL ARTICLE
Xiaoxiao Wei, Hao Zhang, Tianmei Zou, ZhenLei Wang
As a non-contact and three-dimensional (3D) mirror surface measurement method, stereo-deflectometry has been developing rapidly in recent years. For solving the problem of the measurement of large surface inclination with traditional stereo-deflectometry, 3D stitching of stereo-deflectometry based on marker points is proposed. In this method, the 3D points in the subregions under different perspectives were measured. Meanwhile, the 3D coordinates of the marker points on the sample table, which were calculated by binocular stereo vision, were used for coarse stitching, and the ICP algorithm was used for fine stitching...
September 1, 2022: Applied Optics
https://read.qxmd.com/read/36183483/possible-metabolic-interplay-between-quality-of-life-and-fecal-microbiota-in-a-presenior-population-preliminary-results
#39
JOURNAL ARTICLE
Begoña de Cuevillas, José I Riezu-Boj, Itziar Abete, M Angeles Zulet, Cristina Galarregui, Carlos J Gonzalez-Navarro, Fermín I Milagro, J Alfredo Martínez, Santiago Navas-Carretero
OBJECTIVES: The number of people aged ≥60 y is increasing worldwide, so establishing a relationship between lifestyle and health-associated factors, such as gut microbiota in an older population, is important. This study aimed to characterize the gut microbiota of a presenior population, and analyze the association between some bacteria and quality of life with the Short Form (SF) 36 questionnaire. METHODS: Participants were adult men and women ages 50 to 80 y (n = 74)...
2022: Nutrition
https://read.qxmd.com/read/35964334/validity-of-the-activpal-monitor-to-measure-stepping-activity-and-activity-intensity-a-systematic-review
#40
REVIEW
Yanlin Wu, Jennifer L Petterson, Nick W Bray, Derek S Kimmerly, Myles W O'Brien
BACKGROUND: Accumulating step counts and engaging in moderate-to-vigorous intensity physical activity is positively associated with numerous health benefits. The activPAL is a thigh-worn monitor that is frequently used to measure physical activity. RESEARCH QUESTION: Can the activPAL accurately measure stepping activity and identify physical activity intensity? METHODS: We systematically reviewed validation studies examining the accuracy of activPAL physical activity outcomes relative to a criterion measure in adults (>18 years)...
August 6, 2022: Gait & Posture
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