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Trace elements

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https://www.readbyqxmd.com/read/29775664/auto-induction-for-high-level-production-of-biologically-active-reteplase-in-escherichia-coli
#1
Mehrnoosh Fathi-Roudsari, Nader Maghsoudi, Amirhossein Maghsoudi, Sepideh Niazi, Morvarid Soleiman
Reteplase is a third generation tissue plasminogen activator (tPA) with a modified structure and prolonged half-life in comparison to native tPA. As a non-glycosylated protein, reteplase is expressed in Escherichia coli. Due to presence of several disulfide bonds, high level production of reteplase is complicated and needs extra steps for conversion to biologically active form. Auto-induction represents a method for high-yield growth of bacterial cells and higher expression of recombinant proteins. Here we have tried to optimize the auto-induction procedure for soluble and active expression of reteplase in E...
May 15, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29773198/serum-concentrations-of-trace-elements-and-their-relationships-with-paraoxonase-1-in-morbidly-obese-women
#2
Fedra Luciano-Mateo, Noemí Cabré, Martí Nadal, Anabel García-Heredia, Gerard Baiges-Gaya, Anna Hernández-Aguilera, Jordi Camps, Jorge Joven, José Luis Domingo
The metabolic alterations associated with obesity include mineral dysregulation. Essential trace elements are nutrients with a relevant function in a large number of cellular processes and multiple roles in the correct functioning of metabolic enzymes. Paraoxonase-1 (PON1) is an antioxidant and anti-inflammatory enzyme that is compromised in obesity. In the present study, the potential alterations in trace elements in morbidly obese women were assessed in relation to serum PON1 activity and concentration, as well as to other obesity-related comorbidities such as diabetes mellitus and fatty liver...
July 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29773189/effects-of-furosemide-administration-on-the-concentration-of-essential-and-toxic-elements-in-wistar-rats-by-inductively-coupled-plasma-optical-emission-spectrometry
#3
Luiz Raimundo Seneterri Silva Rodrigues Dos Santos, Aníbal de Freitas Santos, Maria das Graças Andrade Korn
Furosemide can interfere with the metabolism of chemical elements, changing their levels in several tissues, thus causing imbalance. In this study, inductively coupled plasma optical emission spectrometry (ICP OES) was used for multi-element analysis (Cd, Cu, Fe, Mg, Pb, Se and Zn) after microwave-assisted digestion, to evaluate the effect of furosemide (loop diuretic) on the composition of these essential and toxic elements in biological samples (liver, kidney, heart, lung and serum) of Wistar rats. Male and female Wistar rats (n = 40, 180-350 g) were randomly divided into 2 groups (n = 20/group)...
July 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29773171/determination-of-toxic-and-essential-trace-elements-in-serum-of-healthy-and-hypothyroid-respondents-by-icp-ms-a-chemometric-approach-for-discrimination-of-hypothyroidism
#4
Aleksandar Stojsavljević, Jelena Trifković, Zorica Rasić-Milutinović, Dragana Jovanović, Gradimir Bogdanović, Jelena Mutić, Dragan Manojlović
Inductively coupled plasma-mass spectrometry ((ICP-MS)) was used to determine three toxic (Ni, As, Cd) and six essential trace elements (Cr, Mn, Co, Cu, Zn, Se) in blood serum of patients with hypothyroidism (Hy group) and healthy people (control group), in order to set the experimental conditions for accurate determination of a unique profile of these elements in hypothyroidism. Method validation was performed with standard reference material of the serum by varying the sample treatment with both standard and collision mode for analysis of elements isotopes...
July 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29772226/potential-role-of-peroxisome-proliferator-activated-receptor-gamma-activation-on-serum-visfatin-and-trace-elements-in-high-fat-diet-induced-type-2-diabetes-mellitus
#5
Arshia Tabassum, Syeda Nuzhat Fatima Zaidi, Kausar Yasmeen, Tabassum Mahboob
AIMS: Electrolytes and trace elements dysregulation play an important role in the progression of obesity and diabetes complications. The present study was designed to evaluate the insulin sensitizing effects of peroxisomes proliferators activated receptor gamma (PPAR-γ) agonist on trace elements in obesity induced type 2 diabetes mellitus and correlate with serum visfatin. MATERIALS AND METHODS: Wistar rats were categorized into five groups. Group I served as control; Group II fed on high fat diet (HFD); Group III fed on HFD and treated with rosiglitazone (3 mg/kg) for 7 days; Group IV were T2DM rats induce by HFD and low dose of streptozotocin (i...
May 14, 2018: Life Sciences
https://www.readbyqxmd.com/read/29771235/bordetella-pertussis-population-dynamics-and-phylogeny-in-japan-after-adoption-of-acellular-pertussis-vaccines
#6
Aldert Zomer, Nao Otsuka, Yukihiro Hiramatsu, Kazunari Kamachi, Naoko Nishimura, Takao Ozaki, Jan Poolman, Jeroen Geurtsen
Bordetella pertussis, the causative agent of whooping cough, has experienced a resurgence in the past 15 years, despite the existence of both whole-cell and acellular vaccines. Here, we performed whole genome sequencing analysis of 149 clinical strains, provided by the National Institute of Infectious Diseases (NIID), Japan, isolated in 1982-2014, after Japan became the first country to adopt acellular vaccines against B. pertussis. Additionally, we sequenced 39 strains provided by the Konan Kosei Hospital in Aichi prefecture, Japan, isolated in 2008-2013...
May 17, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29770110/a-pcr-based-method-for-rna-probes-and-applications-in-neuroscience
#7
Ruifang Hua, Shanshan Yu, Mugen Liu, Haohong Li
In situ hybridization (ISH) is a powerful technique that is used to detect the localization of specific nucleic acid sequences for understanding the organization, regulation, and function of genes. However, in most cases, RNA probes are obtained by in vitro transcription from plasmids containing specific promoter elements and mRNA-specific cDNA. Probes originating from plasmid vectors are time-consuming and not suitable for the rapid gene mapping. Here, we introduce a simplified method to prepare digoxigenin (DIG)-labeled non-radioactive RNA probes based on polymerase chain reaction (PCR) amplification and applications in free-floating mouse brain sections...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29769396/bulk-electroporation-mediated-gene-transfer-into-xenopus-tadpole-brain
#8
Cristina Sáenz de Miera, Ethan Parr, Robert J Denver
In vivo gene transfer is a powerful tool for investigating protein function and gene regulation in living organisms. Delivery of plasmid DNA to the brain of Xenopus tadpoles by bulk electroporation-mediated (EM) gene transfer can be used to study the effects of ectopic gene expression on development, physiology, and behavior. It can also be used to mark cells for lineage tracing, investigate the in vivo function of gene regulatory elements when linked to a reporter gene, and introduce mutations into the genome of transfected cells, among other applications...
May 16, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29768978/levels-of-serum-trace-elements-in-patients-with-peyronie
#9
Mustafa Gunes, Rahmi Aslan, Recep Eryılmaz, Halit Demir, Kerem Taken
AIM: The etiology of the disease of Peyronie is not certainly known. However, penile micro traumas are thought to be important in the pathogenesis of Peyronie's disease (PD) in genetically predisposed individuals. In this study, we aimed to determine the relationship of some trace element and heavy metals with PD. MATERIAL AND METHODS: Thirty Peyronie patients and 26 healthy volunteers were included in the study. In individuals in both groups, levels of serum trace elements (Manganese [Mn], Cu, Cobalt (Co), zinc [Zn], Cd, and iron [Fe]) were determined separately by Atomic Absorption Spectrophotometer method in Yüzüncü Yıl University Central Research Laboratory...
May 17, 2018: Aging Male: the Official Journal of the International Society for the Study of the Aging Male
https://www.readbyqxmd.com/read/29767695/molybdenum
#10
Janet A Novotny, Catherine A Peterson
Molybdenum, a trace element essential for micro-organisms, plants, and animals, was discovered in 1778 by a Swedish chemist named Karl Scheele. Initially mistaken for lead, molybdenum was named after the Greek work molybdos, meaning lead-like. In the 1930s, it was recognized that ingestion of forage with high amounts of molybdenum by cattle caused a debilitating condition. In the 1950s, the essentiality of molybdenum was established with the discovery of the first molybdenum-containing enzymes. In humans, only 4 enzymes requiring molybdenum have been identified to date: sulfite oxidase, xanthine oxidase, aldehyde oxidase, and mitochondrial amidoxime-reducing component (mARC)...
May 1, 2018: Advances in Nutrition
https://www.readbyqxmd.com/read/29767397/the-potential-role-of-spherocrystals-in-the-detoxification-of-essential-trace-metals-following-exposure-to-cu-and-zn-in-the-fighting-conch-strombus-lobatus-pugilis
#11
Jean-Marie Volland, Paco Bustamante, Dalila Aldana Aranda, Olivier Gros
Crypt cells-one of the three cell types composing Strombidae digestive tubules-are characterized by the presence of numerous metal-containing phosphate granules termed spherocrystals. We explored the bioaccumulation and detoxification of metals in Strombidae by exposing wild fighting conch Strombus pugilis for 9 days to waterborne CuSO4 and ZnSO4 . The total amount of Cu and Zn was determined in the digestive gland and in the rest of the body by Inductively Coupled Plasma (ICP) analyses. The digestive gland spherocrystal metal content was investigated based on the semi-quantitative energy dispersive X-ray (EDX) elemental analysis...
May 16, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29766690/garden-nasturtium-tropaeolum-majus-l-a-source-of-mineral-elements-and-bioactive-compounds
#12
Karolina Jakubczyk, Katarzyna Janda, Katarzyna Watychowicz, Jagoda Łukasiak, Jolanta Wolska
The garden nasturtium (Tropaeolum majus L.) belongs to the family Tropaeolaceae. Native to South America it was brought to Europe in XVI century. It is a plant with numerous healing properties. Medicinal plants such as the garden nasturtium contain trace elements and bioactive compounds which can be easily absorbed by the human body. The flowers and other parts of the garden nasturtium are a good source of micro elements such as potassium, phosphorus, calcium and magnesium, and macro elements, especially of zinc, copper and iron...
2018: Roczniki Państwowego Zakładu Higieny
https://www.readbyqxmd.com/read/29766524/a-tool-to-assure-the-geographical-origin-of-local-food-products-glasshouse-tomatoes-using-labeling-with-rare-earth-elements
#13
Donata Bandoniene, Thomas Meisel, Alessandra Rachetti, Christoph Walkner
BACKGROUND: Trace element fingerprinting has been widely used for identification of provenance of regional food. In the case of the products from conventional agriculture, it is expected that the elemental composition will comply that of the commercially available substrate at the plants. Therefore, for products without direct relationship with the regional soil the region-specific differences in elemental composition are no longer recognisable. The idea of this work is labeling of tomatoes with rare earth elements (REE) in the ultra-trace range for food authentication...
May 16, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29764249/the-effects-of-acrylamide-and-vitamin-e-on-kidneys-in-pregnancy-an-experimental-study
#14
Mehmet Erman Erdemli, Zeynep Aksungur, Mehmet Gul, Birgul Yigitcan, Harika Gozukara Bag, Eyup Altinoz, Yusuf Turkoz
OBJECTIVES: The objective of this study is to investigate possible damages to kidney tissues of pregnant rats and their fetuses exposed to acrylamide during pregnancy and possible protective effects of vitamin E against these damages. MATERIAL AND METHODS: Rats were randomly assigned to five groups of control, corn oil, vitamin E, acrylamide, vitamin E + acrylamide, six pregnant rats in each. Mother and fetal kidney tissues were examined for malondialdehyde (MDA), reductase glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS), total oxidant status (TOS), urea, creatine, trace elements such as Zn and Cu in the serum and histopathological analyses were conducted...
May 15, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29762567/chemical-elemental-analysis-of-single-acoustic-levitated-water-droplets-by-laser-induced-breakdown-spectroscopy
#15
Victor Contreras, Ricardo Valencia, Jairo Peralta, H Sobral, M A Meneses-Nava, Horacio Martinez
Laser-induced breakdown spectroscopy is presented for trace element detection of liquid samples by analyzing a single droplet levitated by ultrasonic waves. A single liquid droplet is placed in the node of a standing acoustic wave produced by a uniaxial levitator for further chemical analysis. The acoustic levitator consists of a commercial Langevin-type transducer, attached to a concave mechanical amplifier, and a concave reflector. A micro-syringe was used to manually place individual liquid droplet samples in the acoustic levitation system...
May 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/29758887/arsenic-rich-shallow-groundwater-in-sandy-aquifer-systems-buffered-by-rising-carbonate-waters-a-geochemical-case-study-from-mannar-island-sri-lanka
#16
U G C Bandara, Saranga Diyabalanage, Christian Hanke, Robert van Geldern, Johannes A C Barth, Rohana Chandrajith
Major ion, trace elements, and stable isotope analyses were performed on groundwater samples collected from Mannar Island in the northern Indian Ocean. Arsenic concentrations up to 34μg/L have been observed in groundwater samples from the island. In addition, 23% of extensively used shallow drinking water wells showed higher arsenic contents than the recommended value by the World Health Organization (10μg/L). Groundwater in the island showed pH values between 6.9 and 8.9 and was dominated by Na+ , K+ , Ca2+ , Mg2+ , HCO3 - , Cl- and SO4 2- ...
August 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29758435/possible-impact-of-environmental-policies-in-the-recovery-of-a-ramsar-wetland-from-trace-metal-contamination
#17
Miguel Ángel Álvarez-Vázquez, Serafín Jesús González-Prieto, Ricardo Prego
The Umia-Grove Intertidal Complex is located within the Ria of Arousa (NW Iberian Peninsula). Out of its significance for wintering shorebirds it has been included in the Ramsar List of Wetlands of International Importance as well as in international protection networks such as the European Natura 2000 and Important Bird and Biodiversity Areas-BirdLife International. In a sediment analysis conducted in the year 1999, the wetland was found to have been contaminated by potentially toxic trace metals, to wit: Cr, Cu, Ni, and Pb...
May 11, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29758013/selenium-dependent-antioxidant-enzymes-actions-and-properties-of-selenoproteins
#18
REVIEW
Evangelos Zoidis, Isidoros Seremelis, Nikolaos Kontopoulos, Georgios P Danezis
Unlike other essential trace elements that interact with proteins in the form of cofactors, selenium (Se) becomes co-translationally incorporated into the polypeptide chain as part of 21st naturally occurring amino acid, selenocysteine (Sec), encoded by the UGA codon. Any protein that includes Sec in its polypeptide chain is defined as selenoprotein. Members of the selenoproteins family exert various functions and their synthesis depends on specific cofactors and on dietary Se. The Se intake in productive animals such as chickens affect nutrient utilization, production performances, antioxidative status and responses of the immune system...
May 14, 2018: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29756775/heterogeneous-interaction-of-various-natural-dust-samples-with-isopropanol-as-a-probe-voc
#19
Mohamad Nour Zeineddine, Manolis N Romanias, Véronique Riffault, Frederic Thevenet
The adsorption properties of mineral dust toward organic molecules are poorly characterized so far. Heterogeneous processes between trace gases and mineral particles can affect the oxidative capacity of the atmosphere as well as constitute additional sources or sinks for these species. The current study investigates the adsorption efficiencies of natural dust samples collected from North and West Africa, Saudi Arabia, and Arizona desert regions toward isopropanol (IPA), a common organic pollutant released in significant amounts in the atmosphere, and used here as a probe molecule...
May 14, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29754096/estimating-bioaccessibility-of-trace-elements-in-particles-suspended-in-the-athabasca-river-using-sequential-extraction
#20
Muhammad Babar Javed, William Shotyk
Employing protocols developed for polar snow and ice, water samples were collected upstream, midstream and downstream of open pit bitumen mines and upgraders along the Lower Athabasca River (AR). The purpose was to: i) estimate the bioaccessibility of trace elements associated with particulate matter in the AR using sequential extraction, and ii) determine whether their forms have been measurably impacted by industrial activities. Of the trace metals known to be enriched in bitumen (V, Ni, Mo and Re), a substantial proportion of V (78-93%) and Ni (35-81%) was found in the residual fraction representing stable minerals...
May 10, 2018: Environmental Pollution
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