Morgane Agez, Patrick Schultz, Igor Medina, David J Baker, Matthew P Burnham, Ross A Cardarelli, Leslie C Conway, Kelly Garnier, Stefan Geschwindner, Anders Gunnarsson, Eileen J McCall, Alexandre Frechard, Stéphane Audebert, Tarek Z Deeb, Stephen J Moss, Nicholas J Brandon, Qi Wang, Niek Dekker, Anass Jawhari
KCC2 is a neuron specific K+ -Cl- co-transporter that controls neuronal chloride homeostasis, and is critically involved in many neurological diseases including brain trauma, epilepsies, autism and schizophrenia. Despite significant accumulating data on the biology and electrophysiological properties of KCC2, structure-function relationships remain poorly understood. Here we used calixarene detergent to solubilize and purify wild-type non-aggregated and homogenous KCC2. Specific binding of inhibitor compound VU0463271 was demonstrated using surface plasmon resonance (SPR)...
November 28, 2017: Scientific Reports