Scarleth Silva Costa, Lucas Magno Oliveira Santos, Larissa Chaves Freire, Ana Luiza Filizzola Tedeschi, Naianda Rezende Ribeiro, Mariana Helena Rodrigues Queiroz, Emídio Beraldo Neto, Daniel Carvalho Pimenta, Nathalia Coral Galvani, Gabriel Paulino Luiz, Maria Eduarda de Oliveira, Ricardo Andrez Machado de Ávila, Ana Maria Ravena Severino Carvalho, Bryan Victor Serafim Brigido, Alexandre Barbosa Reis, Ana Paula Salles Moura Fernandes, Eduardo Antonio Ferraz Coelho, Bruno Mendes Roatt, Daniel Menezes-Souza, Mariana Costa Duarte
In the present study, an immunoproteomic approach using Leishmania infantum parasites isolated from naturally infected dogs from an endemic region of the disease, was carried out to identify new antigens to be used in the diagnosis of canine visceral leishmaniasis (CVL). Protein extracts, obtained from parasites isolated from asymptomatic (CanLA) and symptomatic (CanLS) dogs, were used to perform the two-dimensional gels. Western Blotting assays were carried out by employing a pool of sera from dogs with visceral leishmaniasis (CanLA or CanLS), healthy dogs from an endemic area, or dogs with similar diseases associated with cross-reactions (babesiosis and ehrlichiosis)...
February 12, 2023: Acta Tropica