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Tracy Prysliak, Teresia Maina, Jose Perez-Casal
The current avenues for prevention and/or control of Mycoplasma bovis infection in cattle involve antibiotic treatment of affected animals, herd management practices including separation and or culling infected animals, and the use of commercial vaccines, which offer limited protection. Some bacterin vaccines may cause negative reactions; therefore a different approach is needed, such as the use of recombinant vaccines based on protective antigens formulated with effective adjuvants. The role of Th-17 immune responses in protection against bacterial infections has been investigated for several pathogens...
March 2018: Veterinary Immunology and Immunopathology
Paul L Wood, Margaret Steinman, Erdal Erol, Craig Carter, Undine Christmann, Ashutosh Verma
Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses...
2018: PloS One
A Limsatanun, J Sasipreeyajan, S Pakpinyo
Mycoplasma gallisepticum (MG) causes respiratory signs and economic losses in the poultry industry. MG vaccination is one of the effective prevention and control measures that have been used around the world. Our previous study demonstrated that chitosan-adjuvanted MG bacterin could effectively reduce pathological lesions induced by MG and that chitosan could be used as an adjuvant in MG bacterin. The present study determining the efficacy of MG bacterins against the Thai MG strain was based on vaccine programs...
February 15, 2018: Poultry Science
Deborah A Grosenbaugh, Maria Camila Pardo
Forty-four specific pathogen-free beagles, median age 65 days, received two subcutaneous doses of either a commercially available, five-way combination vaccine or the same vaccine in combination with a tetravalent Leptospira bacterin (Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona). They were subsequently challenged with a pathogenic strain L kirschneri serovar Grippotyphosa 470 days following completion of the vaccination protocol. Titres of agglutinating serum antibodies were determined at various time points before and after both vaccination and challenge, along with postchallenge reisolation of the challenge organisms from blood and urine, and evaluation of renal histopathology...
February 19, 2018: Veterinary Record
Hsing-Chieh Wu, Po-Hsien Yeh, Kai-Jen Hsueh, Wen-Jen Yang, Chun-Yen Chu
AIMS: Available bacterins, commercial or autogenous, for Actinobacillus pleuropneumoniae disease control have thus far shown debatable protective efficacy, and only in homologous challenges. Our study sought to determine whether the addition of reombinant protein ApxIV to the multi-component vaccine could enhance protection against homologous and heterologous challenge of A. pleuropneumoniae. METHODS AND RESULTS: The virulence of ApxI, ApxII, ApxIV, and OMP were cloned and expressed using a prokaryotic system; these recombinant proteins were combined with inactivated A...
February 12, 2018: Journal of Applied Microbiology
Didier Duivon, Isabelle Corrégé, Anne Hémonic, Martial Rigaut, David Roudaut, Rika Jolie
Background: A controlled randomized trial was performed on a well-managed conventional French 180-sow farm. The trial compared the growth performances of piglets vaccinated at weaning (single shot) either with a commercial monovalent Mycoplasma hyopneumoniae bacterin vaccine or with a commercial bivalent vaccine (Porcilis® PCV M Hyo) against M. hyopneumoniae and porcine circovirus 2 (PCV2). The farm's porcine reproductive and respiratory syndrome status was stable, and most diseases (enzootic pneumonia, atrophic rhinitis, post-weaning multisystemic wasting syndrome) were controlled by routine vaccination...
2018: Porcine Health Management
Pallop Tankaew, Wanwisa Srisawat, Tawatchai Singhla, Khajornsak Tragoolpua, Yasushi Kataoka, Takuo Sawada, Nattawooti Sthitmatee
The ELISA is recognized as an efficient diagnostic tool for antibody detection, but there is no standard ELISA assay for detection of antibodies against hemorrhagic septicemia (HS) in cattle. The present study reports on an indirect ELISA assay for antibody detection of HS in dairy cows, and evaluates the sensitivity (Se) and specificity (Sp) of the method using a Bayesian approach. An indirect ELISA was developed with two types of heat extract antigens, Pasteurella multocida strains P-1256 and M-1404, as coating antigens...
December 12, 2017: Journal of Microbiological Methods
Rafael Bazaglia Sonada, Sérgio Santos de Azevedo, Francisco Rafael Martins Soto, Diego Figueiredo da Costa, Zenaide Maria de Morais, Gisele Oliveira de Souza, Amane Paldês Gonçales, Fabiana Miraglia, Sílvio Arruda Vasconcellos
In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR...
October 12, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Ananda Paula Kowalski, Grazieli Maboni, Letícia Trevisan Gressler, Julia Pires Espíndola, Cláudia Balzan, Caiane Tasca, João Antônio Guizzo, Fabricio Rochedo Conceição, Rafael Frandoloso, Agueda Castagna de Vargas
Moraxella bovis is historically known as the primary agent of infectious bovine keratoconjunctivitis (IBK). However, Moraxella bovoculi and Moraxella ovis are also reported to be involved in the pathogenesis of IBK, therefore, these three species should be included in the development of a new vaccine with a broad-spectrum protection against the disease natural challenge. In this study we investigated the antigenic properties of clinical isolates and reference strains of M. bovis, M. bovoculi and M. ovis using a novel in vitro approach for vaccine evaluation based on two techniques, flow cytometry and western blotting (WB)...
October 2017: Veterinary Microbiology
Rzgar M Jaafar, Azmi Al-Jubury, Inger Dalsgaard, Asma MohammadKarami, Per W Kania, Kurt Buchmann
Vaccination of rainbow trout against Enteric Redmouth Disease (ERM) caused by Yersinia ruckeri can be successfully performed by administering vaccine (a bacterin consisting of formalin killed bacteria) by immersion, bath or injection. Booster immunization is known to increase the protection of fish already primed by one of these vaccination methods. Oral vaccination of trout (administering vaccine in feed) is an even more convenient way of presenting antigen to the fish but the effect of an oral booster has not previously been described in detail...
October 30, 2017: Fish & Shellfish Immunology
Tracy Prysliak, Teresia Maina, Lu Yu, Muhammad Suleman, Steve Jimbo, Jose Perez-Casal
Prevention and or control of Mycoplasma bovis infections in cattle have relied on the treatment of animals with antibiotics; herd management including separation and or culling infected animals; and the use of vaccines with limited protection. Due to the negative reactions and incomplete protection observed after vaccination with some bacterin-based vaccines, there is a need to put more efforts in the development of recombinant-based vaccines. However, the arsenal of antigens that may be suitable for a fully protective vaccine is rather limited at this point...
October 24, 2017: Vaccine
A K Ray, C Gopal, H G Solanki, T Ravisankar, P K Patil
Vibriosis is one of the important diseases causing economic loss to the shrimp industry worldwide. The present study reports field observations on the immune stimulatory effect of vibrio bacterin in commercial tiger shrimp (Penaeus monodon) grow-out culture ponds (n = 62) which were grouped under three stocking densities; low (6-8 nos per m(2) ), medium (9-11 nos per m(2) ) and high (12-14 nos per m(2) ). The bacterin was administered in feed as a top dressing at final concentration equivalent to 2 × 10(8) CFU per kilogram feed twice a week throughout the culture period...
October 6, 2017: Letters in Applied Microbiology
Ali Reza Khansari, David Parra, Felipe E Reyes-López, Lluís Tort
In fish, the stress response and their consequences in the immune system have been widely described. Recently, a differential cytokine regulation between rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) was reported after treatment with stress hormones together with their receptor antagonists. Nevertheless, there is no evidence of whether antagonists for stress hormone receptors may influence the interaction between hormones and cytokines after bacterial administration. Thus, the aim of our study was to evaluate the cytokine expression in the presence of stress hormones (cortisol, ACTH, adrenaline), hormone receptor antagonists and inactivated Vibrio anguillarum bacterin in rainbow trout and gilthead sea bream head kidney primary cell culture (HKPCC)...
September 4, 2017: Fish & Shellfish Immunology
Pichayanut Poolperm, Thanya Varinrak, Yasushi Kataoka, Khajornsak Tragoolpua, Takuo Sawada, Nattawooti Sthitmatee
Serological tests, such as agglutination and indirect hemagglutination assay (IHA), have been used to identify antibodies against Pasteurella multocida in poultry sera, but none are highly sensitive. An enzyme-linked immunosorbent assays (ELISA) has been used with varying degrees of success in attempts to monitor seroconversion in vaccinated poultry, but are not suitable for diagnosis. Commercial ELISA kits are available for chickens and turkeys, but not for ducks. The present study reports development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera...
November 2017: Journal of Microbiological Methods
Caihong Liu, Yurou Cao, Jihong Yang, Haobin Zhao, Kashif Rafiq Zahid, Jin Zhao, Chao Qi, Jinlin Liu
Identification of immunogenic antigens is an important step for the vaccine improvement of infectious diseases. Previous studies indicated that Actinobacillus pleuropneumoniae PalA is homologous to a Haemophilus influenzae protective antigen Hi-PAL (P6) protein. However, PalA protein adversely affects the Apx toxin-based subunit vaccine. In this study, we confirmed that recombinant PalA is immunogenic in animals. Mice immunized with bacterin and PalA (BacPal) showed significantly less antibodies against Apx toxins, relative to that of animals immunized with bacterin alone (Bac) before challenge...
August 22, 2017: Protein and Peptide Letters
Eakapol Wangkahart, Christopher J Secombes, Tiehui Wang
Enteric redmouth disease (ERM or yersiniosis) is one of the most important diseases of salmonids and leads to significant economic losses. It is caused by the Gram-negative bacterium Yersinia ruckeri but can be controlled by bacterin vaccination. The first commercial ERM vaccine was licenced in 1976 and is one of the most significant and successful health practices within the aquaculture industry. Although ERM vaccination provides complete protection, knowledge of the host immune response to the vaccine and the molecular mechanisms that underpin the protection elicited is limited...
July 27, 2017: Fish & Shellfish Immunology
M Parra, B Valenzuela, M Imarai, B Modak
No abstract text is available yet for this article.
July 24, 2017: Journal of Fish Diseases
Kasper Rømer Villumsen, Erling Olaf Koppang, Dennis Christensen, Anders Miki Bojesen
In an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. Using rainbow trout (Oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated Aeromonas salmonicida subspecies salmonicida were formulated with CpG oligodeoxynucleotides, the liposomal cationic adjuvant formulation 01 (CAF01) or with Freund's incomplete adjuvant and administered intraperitoneally...
July 19, 2017: Scientific Reports
R M Jaafar, A Al-Jubury, J K Chettri, I Dalsgaard, P W Kania, K Buchmann
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been described that repeated injection vaccination of fish raises a secondary immune response, consisting of rapid, accelerated and increased antibody reaction. This study reports how rainbow trout responds to repeated immersion vaccination against yersiniosis (ERM) caused by the bacterial pathogen Yersinia ruckeri...
July 14, 2017: Journal of Fish Diseases
Cassandra Guarino, Sanda Asbie, Jennifer Rohde, Amy Glaser, Bettina Wagner
Borrelia burgdorferi can induce Lyme disease. Approved Lyme vaccines for horses are currently not available. In an effort to protect horses, veterinarians are using Lyme vaccines licensed for dogs. However, data to assess the response of horses to, or determine the efficacy of this off-label vaccine use are missing. Here, antibodies against outer surface protein A (OspA), OspC, and OspF were quantified in diagnostic serum submissions from horses with a history of vaccination with canine Lyme vaccines. The results suggested that many horses respond with low and often short-lasting antibody responses...
July 24, 2017: Vaccine
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