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https://www.readbyqxmd.com/read/28791613/oleic-acid-enhances-dengue-virus-but-not-dengue-virus-like-particle-production-from-mammalian-cells
#1
Suwipa Ramphan, Sathiporn Suksathan, Nitwara Wikan, Puey Ounjai, Kanpong Boonthaworn, Poramate Rimthong, Tapanee Kanjanapruthipong, Suchin Worawichawong, Anan Jongkaewwattana, Nuttaporn Wongsiriroj, Duncan R Smith
Despite the recent introduction of a commercial vaccine, the mosquito-transmitted dengue virus is still a worldwide public health problem. Based on the live attenuated vaccine strategy, the commercial vaccine has a less than optimal protective profile. Virus-like particles (VLPs) offer an attractive alternate vaccination strategy due to the effectively native presentation of epitopes in the absence of any infectious genetic material. However, the production of amounts of VLP in a platform that can support commercial development remains a major obstacle...
August 8, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28770248/expression-of-domain-iii-of-the-envelope-protein-from-gp-78-a-japanese-encephalitis-virus
#2
Sahil Kulkarni, Sandeepan Mukherjee, Krishna Mohan Padmanabha Das, Kaushiki Prabhudesai, Nupur Deshpande, Sushant Karnik, Abhay S Chowdhary, Usha Padmanabhan
Acute encephalitis caused by the Japanese encephalitis virus (JEV) represents a growing epidemic and is a cause for concern in Southeast Asia. JEV is transmitted to humans through the bite of the Culicine mosquito species. The virus genome comprising of an RNA strand also encodes the envelope protein (E) which surrounds the virus. The E protein aids in fusion of virus with the cellular membrane of the host cell with the help of three structurally distinct domains (DI, DII, DIII) that are connected by flexible hinge regions...
June 2017: Virusdisease
https://www.readbyqxmd.com/read/28748833/identification-and-characterization-of-differentially-expressed-genes-from-human-microglial-cell-samples-infected-with-japanese-encephalitis-virus
#3
Manoj Kumar Gupta, Santosh Kumar Behera, Budheswar Dehury, Namita Mahapatra
BACKGROUND & OBJECTIVES: Limited studies have been reported on Japanese encephalitis (JE) with reference to microarray data analysis. The present study involved an in silico approach for identification and characterization of differentially expressed genes in human microglial cell (CHME3) samples, infected with P20778 strain of Japanese encephalitis virus (JEV). METHODS: Gene expression data (GSE57330) belonging to mRNA expression profile of CHME3 cells infected with JEV, was downloaded from the gene expression omnibus (GEO) database, processed and normalized by robust multichip averaging (RMA) method using affy packages of R...
April 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28740748/a-highly-pathogenic-porcine-reproductive-and-respiratory-syndrome-virus-candidate-vaccine-based-on-japanese-encephalitis-virus-replicon-system
#4
Pingsheng Hu, Xiaoming Chen, Lihong Huang, Shukai Liu, Fuyu Zang, Jinchao Xing, Youyue Zhang, Jiaqi Liang, Guihong Zhang, Ming Liao, Wenbao Qi
In the swine industry, porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease which causes heavy economic losses worldwide. Effective prevention and disease control is an important issue. In this study, we described the construction of a Japanese encephalitis virus (JEV) DNA-based replicon with a cytomegalovirus (CMV) promoter based on the genome of Japanese encephalitis live vaccine virus SA14-14-2, which is capable of offering a potentially novel way to develop and produce vaccines against a major pathogen of global health...
2017: PeerJ
https://www.readbyqxmd.com/read/28728201/-fever-and-headache-after-a-vacation-in-thailand
#5
Benedikt Schwermer, Daniel Eschle, Constantine Bloch-Infanger
History 50 year-old man with fever and headache starting one week after returning from his vacation in Thailand. His general practitioner prescribed amoxicillin/clavulanic acid, without further analyses to pinpoint the infection. Examinations The examination of cerebro-spinal fluid was crucial for the final diagnosis. Lumbar puncture demonstrated a predominantly mononuclear pleocytosis of 80 cells/µl (< 5) with an elevated protein of 782 mg/l (< 450); glucose and lactate were within normal limits...
July 2017: Deutsche Medizinische Wochenschrift
https://www.readbyqxmd.com/read/28724764/rab5-and-rab11-are-required-for-clathrin-dependent-endocytosis-of-japanese-encephalitis-virus-in-bhk-21-cells
#6
Chun-Chun Liu, Yun-Na Zhang, Zhao-Yao Li, Jin-Xiu Hou, Jing Zhou, Lin Kan, Bin Zhou, Pu-Yan Chen
During infection Japanese Encephalitis Virus generally enters host cells via receptor-mediated clathrin dependent endocytosis. The trafficking of JEV within endosomes is controlled by Rab GTPases, but which Rab proteins are involved in JEV entry into BHK-21 cells is unknown. In this study, entry and post-internalization of JEV were analyzed using biochemical inhibitors, RNA interference and dominant negative mutants. Our data demonstrate that JEV entry into BHK-21 cells depends on clathrin, dynamin, and cholesterol, but not caveolae or macropinocytosis...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28710958/japanese-encephalitis-virus-counteracts-bst2-restriction-via-its-envelope-protein-e
#7
Mei Li, Ping Wang, Zifeng Zheng, Kai Hu, Mudan Zhang, Xinmeng Guan, Ming Fu, Di Zhang, Wei Wang, Gengfu Xiao, Qinxue Hu, Yalan Liu
It has been well documented that BST2 restricts the release of enveloped viruses by cross-linking newly produced virions to the cell membrane. However, it is less clear whether and how BST2 inhibits the release of enveloped viruses which bud via the secretory pathway. Here, we demonstrated that BST2 restricts the release of Japanese encephalitis virus (JEV) whose budding occurs at the ER-Golgi intermediate compartment, and in turn, JEV infection downregulates BST2 expression. We further found that the JEV envelope protein E, but not other viral components, significantly downregulates BST2 with the viral protein M playing an auxiliary role in the process...
July 12, 2017: Virology
https://www.readbyqxmd.com/read/28705180/etiology-and-prognosis-of-acute-viral-encephalitis-and-meningitis-in-chinese-children-a-multicentre-prospective-study
#8
Junhong Ai, Zhengde Xie, Gang Liu, Zongbo Chen, Yong Yang, Yuning Li, Jing Chen, Guo Zheng, Kunling Shen
BACKGROUND: In China, there were few studies about the pathogens of acute viral encephalitis and meningitis in children in recent years. The aims of this study were to characterize the etiology and prognosis of acute viral encephalitis and meningitis in Chinese children. METHODS: This was a multicentre prospective study. Two hundred and sixty one viral encephalitis patients and 285 viral meningitis patients were enrolled. The mean age of viral encephalitis and meningitis were 5...
July 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28693301/serological-and-molecular-epidemiology-of-japanese-encephalitis-virus-infections-in-swine-herds-in-china-2006-2012
#9
Chunxia Chai, Qiao Wang, Sanjie Cao, Qin Zhao, Yiping Wen, Xiaobo Huang, Xintian Wen, Qiguai Yan, Xiaoping Ma, Rui Wu
Japanese encephalitis virus (JEV) is a mosquito-borne, zoonotic flavivirus causing viral encephalitis in humans and reproductive disorder in pig. JEV is widely prevalent throughout China in human, however, studies on JEV spatiotemporal analysis in Chinese swine herds have not been explored. Here, we present our serological and molecular epidemiological findings of the prevalence of JEV infections among swine herds in China, which suggest that JEV infections are extensively widespread and genotype I and III strains co-exist...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28690034/interplay-of-inflammatory-gene-expression-in-pericytes-following-japanese-encephalitis-virus-infection
#10
Cheng-Yi Chang, Jian-Ri Li, Yen-Chuan Ou, Shih-Yi Lin, Ya-Yu Wang, Wen-Ying Chen, Yu-Hui Hu, Ching-Yi Lai, Chen-Jung Chang, Chun-Jung Chen
Neuroinflammation is a pathological hallmark and has been implicated in the pathogenesis of Japanese encephalitis. Although brain pericytes show regulatory effects on neuroinflammation, their involvement in Japanese encephalitis-associated neuroinflammation is not understood. Here, we demonstrated that brain microvascular pericytes could be an alternative cellular source for the induction and/or amplification of neuroinflammation caused by Japanese encephalitis virus (JEV) infection. Infection of cultured pericytes with JEV caused profound production of IL-6, RANTES, and prostaglandin E2 (PGE2)...
July 6, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28680856/sertoli-cells-are-susceptible-to-zikv-infection-in-mouse-testis
#11
Zi-Yang Sheng, Na Gao, Zhao-Yang Wang, Xiao-Yun Cui, De-Shan Zhou, Dong-Ying Fan, Hui Chen, Pei-Gang Wang, Jing An
Flaviviruses including Dengue virus (DENV), Yellow fever virus (YFV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) are global health problems that caused several serious diseases such as fever, hemorrhagic fever, and encephalitis in the past century. Recently, Zika virus (ZIKV) which spreads from Asia to American and causes millions of infections emerges as a new dangerous member of the genus of Flavivirus. Unlike other well-known flaviviruses, ZIKV can be transmitted sexually and infect testes in murine models...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28680855/p21-activated-kinase-4-signaling-promotes-japanese-encephalitis-virus-mediated-inflammation-in-astrocytes
#12
Wen He, Zikai Zhao, Awais Anees, Yunchuan Li, Usama Ashraf, Zheng Chen, Yunfeng Song, Huanchun Chen, Shengbo Cao, Jing Ye
Japanese encephalitis virus (JEV) targets central nervous system, resulting in neuroinflammation with typical features of neuronal death along with hyper activation of glial cells. Exploring the mechanisms responsible for the JEV-caused inflammatory response remains a pivotal area of research. In the present study, we have explored the function of p21-activated kinase 4 (PAK4) in JEV-mediated inflammatory response in human astrocytes. The results showed that JEV infection enhances the phosphorylation of PAK4 in U251 cells and mouse brain...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28673857/development-and-application-of-a-reverse-transcriptase-droplet-digital-pcr-rt-ddpcr-for-sensitive-and-rapid-detection-of-japanese-encephalitis-virus
#13
Xulong Wu, Hua Lin, Shijie Chen, Lu Xiao, Miao Yang, Wei An, Yin Wang, Xueping Yao, Zexiao Yang
Japanese encephalitis (JE) is one of the most common zoonoses caused by Japanese encephalitis virus (JEV). Droplet digital PCR (ddPCR) is a novel sensitive, accurate method that enables absolute quantitation without the need for calibration curves. The aim of this study was to develop a RT-ddPCR method to detect JEV, and to compare its sensitivity with real-time TaqMan RT-PCR by analysis of clinical samples. The methods of JEV real-time RT-PCR and RT-ddPCR were established and optimal reaction conditions were confirmed...
June 30, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28656253/heat-shock-protein-90%C3%AE-in-the-vero-cell-membrane-binds-japanese-encephalitis-virus
#14
Yuan Wang, Yan Li, Tianbing Ding
The pathogenesis of Japanese encephalitis virus (JEV) is complex and unclearly defined, and in particular, the effects of the JEV receptor (JEVR) on diverse susceptible cells are elusive. In contrast to previous studies investigating JEVR in rodent or mosquito cells, in this study, we used primate Vero cells instead. We noted that few novel proteins co‑immunoprecipitated with JEV, and discovered that one of these was heat shock protein 90β (HSP90β), which was probed by mass spectrometry with the highest score of 60...
June 26, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28619100/cyclophilin-b-facilitates-the-replication-of-orf-virus
#15
Kui Zhao, Jida Li, Wenqi He, Deguang Song, Ximu Zhang, Di Zhang, Yanlong Zhou, Feng Gao
BACKGROUND: Viruses interact with host cellular factors to construct a more favourable environment for their efficient replication. Expression of cyclophilin B (CypB), a cellular peptidyl-prolyl cis-trans isomerase (PPIase), was found to be significantly up-regulated. Recently, a number of studies have shown that CypB is important in the replication of several viruses, including Japanese encephalitis virus (JEV), hepatitis C virus (HCV) and human papillomavirus type 16 (HPV 16). However, the function of cellular CypB in ORFV replication has not yet been explored...
June 15, 2017: Virology Journal
https://www.readbyqxmd.com/read/28592296/pigsties-near-dwellings-as-a-potential-risk-factor-for-the-prevalence-of-japanese-encephalitis-virus-in-adult-in-shanxi-china
#16
Xiaojie Ren, Shihong Fu, Peifang Dai, Huanyu Wang, Yuanyuan Li, Xiaolong Li, Wenwen Lei, Xiaoyan Gao, Ying He, Zhi Lv, Jingxia Cheng, Guiqin Wang, Guodong Liang
BACKGROUND: The increasing trend of adult cases of Japanese encephalitis (JE) in China, particularly in northern China, has become an important public health issue. We conducted an epidemiological investigation in the south of Shanxi Province to examine the relationships between mosquitoes, Japanese encephalitis virus (JEV), and adult JE cases. METHODS: Mosquito specimens were collected from the courtyards of farmers' households and pig farms in Shanxi Province...
June 8, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28559152/anti-apoptotic-activity-of-japanese-encephalitis-virus-ns5-protein-in-human-medulloblastoma-cells-treated-with-interferon-%C3%AE
#17
Jing-Ru Weng, Chun-Hung Hua, Chao-Hsien Chen, Su-Hua Huang, Ching-Ying Wang, Ying-Ju Lin, Lei Wan, Cheng-Wen Lin
BACKGROUND: Japanese encephalitis virus (JEV) non-structural protein 5 (NS5) exhibits type I interferon (IFN) antagonists, contributing to immune escape, and even inducing viral anti-apoptosis. This study investigated the anti-apoptotic mechanism of JEV NS5 protein on type I IFN-induced apoptosis of human medulloblastoma cells. METHODS: Vector control and NS5-expressing cells were treated with IFN-β, and then harvested for analyzing apoptotic pathways with flow cytometry, Western blotting, subcellular localization, etc...
May 19, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28542611/no-molecular-or-serological-evidence-of-zikavirus-infection-among-healthy-blood-donors-living-in-or-travelling-to-regions-where-aedes-albopictus-circulates
#18
Wegene Borena, Tamara Hofer, Karin Stiasny, Stephan W Aberle, Manfred Gaber, Dorothee von Laer, Harald Schennach
BACKGROUND: Previous studies have shown that Zika virus can infect and be transmitted by A. albopictus. The World Health organization (WHO) has raised concerns of autochthonous transmission of the virus in regions where the vector is endemic. The aim of this pilot study was to assess the occurrence of Zika virus (ZIKV) in western Austria (Tyrol) especially after a history of travel to A. albopictus endemic regions. METHODS: The study participants were healthy blood donors at randomly selected donation sites in the west Austrian region Tyrol...
2017: PloS One
https://www.readbyqxmd.com/read/28536430/aetiology-of-acute-meningoencephalitis-in-cambodian-children-2010-2013
#19
Paul F Horwood, Veasna Duong, Denis Laurent, Channa Mey, Heng Sothy, Ky Santy, Beat Richner, Seiha Heng, Sopheak Hem, Justine Cheval, Christopher Gorman, Philippe Dussart, Menno D de Jong, Alexandra Kerleguer, Bertrand Guillard, Bernadette Murgue, Marc Lecuit, Xavier de Lamballerie, Jeremy J Farrar, Arnaud Tarantola, Marc Eloit, Philippe Buchy
Acute meningoencephalitis (AME) is associated with considerable morbidity and mortality in children in developing countries. Clinical specimens were collected from children presenting with AME at two Cambodian paediatric hospitals to determine the major aetiologies associated with AME in the country. Cerebrospinal fluid (CSF) and blood samples were screened by molecular and cell culture methods for a range of pathogens previously associated with AME in the region. CSF and serum (acute and convalescent) were screened for antibodies to arboviruses such as Japanese encephalitis virus (JEV), dengue virus (DENV), and chikungunya virus (CHIKV)...
May 24, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28535855/japanese-encephalitis-virus-activates-autophagy-through-xbp1-and-atf6-er-stress-sensors-in-neuronal-cells
#20
Manish Sharma, Sankar Bhattacharyya, Kiran Bala Sharma, Shailendra Chauhan, Suramya Asthana, Malik Zainul Abdin, Sudhanshu Vrati, Manjula Kalia
Endoplasmic reticulum (ER) stress and autophagy are key cellular responses to RNA virus infection. Recent studies have shown that Japanese encephalitis virus (JEV)-induced autophagy negatively influences virus replication in mouse neuronal cells and embryonic fibroblasts, and delays virus-induced cell death. Here, we evaluated the role of ER stress pathways in inducing autophagy during JEV infection. We observed that JEV infection of neuronal cells led to activation of all three sensors of ER stress mediated by eIF2α/PERK, IRE1/XBP1 and ATF6...
May 2017: Journal of General Virology
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