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https://www.readbyqxmd.com/read/27892528/sphingomyelin-generated-by-sphingomyelin-synthase-1-is-involved-in-attachment-and-infection-with-japanese-encephalitis-virus
#1
Makoto Taniguchi, Takafumi Tasaki, Hideaki Ninomiya, Yoshibumi Ueda, Koh-Ichi Kuremoto, Susumu Mitsutake, Yasuyuki Igarashi, Toshiro Okazaki, Tsutomu Takegami
Japanese encephalitis virus (JEV) is a mosquito-borne RNA virus which infects target cells via the envelope protein JEV-E. However, its cellular targets are largely unknown. To investigate the role of sphingomyelin (SM) in JEV infection, we utilized SM-deficient immortalized mouse embryonic fibroblasts (tMEF) established from SM synthase 1 (SMS1)/SMS2 double knockout mice. SMS deficiency significantly reduced both intracellular and extracellular JEV levels at 48 h after infection. Furthermore, after 15 min treatment with JEV, the early steps of JEV infection such as attachment and cell entry were also diminished in SMS-deficient tMEFs...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27834908/epitope-identification-and-application-for-diagnosis-of-duck-tembusu-virus-infections-in-ducks
#2
Chenxi Li, Junyan Liu, Wulin Shaozhou, Xiaofei Bai, Qingshan Zhang, Ronghong Hua, Jyung-Hurng Liu, Ming Liu, Yun Zhang
Duck Tembusu virus (DTMUV) causes substantial egg drop disease. DTMUV was first identified in China and rapidly spread to Malaysia and Thailand. The antigenicity of the DTMUV E protein has not yet been characterized. Here, we investigated antigenic sites on the E protein using the non-neutralizing monoclonal antibodies (mAbs) 1F3 and 1A5. Two minimal epitopes were mapped to (221)LD/NLPW(225) and (87)YAEYI(91) by using phage display and mutagenesis. DTMUV-positive duck sera reacted with the epitopes, thus indicating the importance of the minimal amino acids of the epitopes for antibody-epitope binding...
November 10, 2016: Viruses
https://www.readbyqxmd.com/read/27828763/serological-prevalence-against-japanese-encephalitis-virus-serocomplex-flaviviruses-in-commensal-and-field-rodents-in-south-china
#3
Shao-Wei Chen, Li-Na Jiang, Xue-Shan Zhong, Xue-Yan Zheng, Shu-Juan Ma, Yi-Quan Xiong, Jun-Hua Zhou, Xing Li, Xue-Mei Ke, Wen Zhou, Qing Chen
BACKGROUND: Japanese encephalitis caused by Japanese encephalitis virus (JEV) is an endemic zoonotic disease of high public health importance in the Asian Pacific region. The aim of this study was to investigate the presence of JEV infection in commensal and field rodents in South China. MATERIALS AND METHODS: RNA copies of JEV were detected in brain samples of rodents using real-time RT-PCR. Detection of serum against JEV-reactive antibodies was performed using indirect enzyme-linked immunosorbent assay and microneutralization test...
December 2016: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/27795474/an-ecological-survey-of-mosquitoes-and-the-distribution-of-japanese-encephalitis-virus-in-ishikawa-prefecture-japan-between-2010-and-2014
#4
Manabu Murakami, Kiyoe Hori, Yoko Kitagawa, Yosaburo Oikawa, Kiyoshi Kamimura, Tsutomu Takagemi
Japanese encephalitis virus (JEV) is a flavivirus and is responsible for over 30,000 annual cases of encephalitis worldwide, with a mortality rate of approximately 30%. Therefore, it is important to check the distribution of JEV in fields, although recently, the number of Japanese encephalitis (JE) cases has been approximately five per year in Japan. We report the seasonal dynamics of mosquitoes between 2010 and 2014 in Ishikawa Prefecture, Japan. We collected 39,308 female adult mosquitoes, 98.2% of which were classified as Culex tritaeniorhynchus Giles...
October 31, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27795473/dengue-associated-acute-encephalitis-syndrome-cases-in-son-la-province-vietnam-in-2014
#5
Mya Myat Ngwe Tun, Rohitha V Muthugala, Nguyen Thi Thu Thuy, Pham Hoai Linh Ly, Le Thi Hien Thu, Dang Thi Dinh, Nguyen Viet Hoang, Le Thi Quynh Mai, Meng Ling Moi, Corazon C Buerano, Kouichi Morita, Futoshi Hasebe
Acute encephalitis syndrome (AES) is associated with high morbidity and mortality, and affects both children and adults. The main etiologic agent is Japanese encephalitis virus (JEV), however there are also reports on Dengue virus (DENV) encephalitis cases. The objectives of this paper were to determine the proportion of encephalitic patients due to JEV during the 2014 outbreak in Son La Province in Vietnam and to find the association of DENV in non-JEV viral encephalitis cases. Out of 90 patients, 6 (6.7%) were positive for anti-JEV IgM, 5 (5...
October 31, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27776548/fast-type-i-interferon-response-protects-astrocytes-from-flavivirus-infection-and-virus-induced-cytopathic-effects
#6
Richard Lindqvist, Filip Mundt, Jonathan D Gilthorpe, Silke Wölfel, Nelson O Gekara, Andrea Kröger, Anna K Överby
BACKGROUND: Neurotropic flaviviruses such as tick-borne encephalitis virus (TBEV), Japanese encephalitis virus (JEV), West Nile virus (WNV), and Zika virus (ZIKV) are causative agents of severe brain-related diseases including meningitis, encephalitis, and microcephaly. We have previously shown that local type I interferon response within the central nervous system (CNS) is involved in the protection of mice against tick-borne flavivirus infection. However, the cells responsible for mounting this protective response are not defined...
October 24, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27774302/inference-of-japanese-encephalitis-virus-ecological-and-evolutionary-dynamics-from-passive-and-active-virus-surveillance
#7
Truc T Pham, Shengli Meng, Yan Sun, Wenli Lv, Justin Bahl
A comprehensive monitoring strategy is vital for tracking the spread of mosquito-borne Japanese encephalitis virus (JEV), the leading cause of viral encephalitis in Asia. Virus detection consists of passive surveillance of primarily humans and swine, and/or active surveillance in mosquitoes, which may be a valuable proxy in providing insights into ecological processes underlying the spread and persistence of JEV. However, it has not been well characterized whether passive surveillance alone can capture the circulating genetic diversity to make reasonable inferences...
January 2016: Virus Evolution
https://www.readbyqxmd.com/read/27773472/immunogenicity-of-live-attenuated-japanese-encephalitis-sa-14-14-2-vaccine-among-sri-lankan-children-with-previous-receipt-of-inactivated-je-vaccine
#8
Pushpa Ranjan Wijesinghe, M R Nihal Abeysinghe, Sutee Yoksan, Yafu Yao, Benli Zhou, Lei Zhang, Jessica A Fleming, Anthony A Marfin, John C Victor
BACKGROUND: The performance of live attenuated Japanese Encephalitis SA 14-14-2 vaccine (CD-JEV) among children previously given inactivated mouse brain-derived JE vaccine (IMBV) is unknown. We evaluated the safety and immunogenicity of CD-JEV administered to 2- and 5-year-old children in Sri Lanka. METHODS: In this open-label, single arm trial in the Colombo District of Sri Lanka, generally healthy children 2 and 5years of age who had previously received two and three doses of IMBV, respectively, were administered one dose of CD-JEV subcutaneously...
November 21, 2016: Vaccine
https://www.readbyqxmd.com/read/27756212/japanese-encephalitis-vaccine-facilitated-dengue-virus-infection-enhancement-antibody-in-adults
#9
Yuka Saito, Meng Ling Moi, Nozomi Takeshita, Chang-Kweng Lim, Hajime Shiba, Kuniaki Hosono, Masayuki Saijo, Ichiro Kurane, Tomohiko Takasaki
BACKGROUND: Dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the genus Flavivirus, and infection with a virus within this genus induces antibodies that are cross-reactive to other flaviviruses. Particularly in DENV infection, antibodies to DENV possess two competing activities: neutralizing activity and infection-enhancing activity. These antibody activities are considered central in modulating clinical outcomes of DENV infection. Here, we determined the neutralizing and infection-enhancing activity of DENV cross-reactive antibodies in adults before and after JE vaccination...
October 18, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27743649/electroporation-enhances-protective-immune-response-of-a-dna-vaccine-against-japanese-encephalitis-in-mice-and-pigs
#10
Ziyang Sheng, Na Gao, Xiaoyun Cui, Dongying Fan, Hui Chen, Na Wu, Jianchun Wei, Jing An
Japanese encephalitis virus (JEV) is a pathogenic cause of Japanese Encephalitis (JE), which is a zoonotic disease transmitted by mosquitoes and amplified by pigs. Infection of JEV may lead to severe neurological sequelae, even death in humans and reproductive disorders in pigs. Vaccination is the only way to control JEV infection in humans. For pigs play important role in the JEV transmission cycle, developing a new veterinary vaccine is considered as a useful strategy for cutting off the transmission route of JEV...
October 12, 2016: Vaccine
https://www.readbyqxmd.com/read/27725612/sensitive-impedimetric-immunoassay-of-japanese-encephalitis-virus-based-on-enzyme-biocatalyzed-precipitation-on-a-gold-nanoparticle-modified-screen-printed-carbon-electrode
#11
Xiaohui Geng, Fanglin Zhang, Qiang Gao, Yingfeng Lei
A sensitive and disposable electrochemical impedance biosensor to detect Japanese encephalitis virus (JEV) was developed based on a gold nanoparticle (AuNP)-modified screen-printed carbon electrode (SPCE). A biosensor was fabricated through covalent grafting of a mixed self-assembled monolayer on AuNPs with a specific antibody. To detect JEV and achieve signal amplification, the horseradish peroxidase (HRP)-labeled second antibody was linked to the biosensor through a sandwich immunity reaction. HRP was used to catalyze 4-chloro-1-naphthol oxidation to produce an insoluble precipitate, which introduced a barrier to electron transfer on the electrode...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27706157/differential-infectivities-among-different-japanese-encephalitis-virus-genotypes-in-culex-quinquefasciatus-mosquitoes
#12
Yan-Jang S Huang, Susan M Hettenbach, So Lee Park, Stephen Higgs, Alan D T Barrett, Wei-Wen Hsu, Julie N Harbin, Lee W Cohnstaedt, Dana L Vanlandingham
During the last 20 years, the epidemiology of Japanese encephalitis virus (JEV) has changed significantly in its endemic regions due to the gradual displacement of the previously dominant genotype III (GIII) with clade b of GI (GI-b). Whilst there is only limited genetic difference distinguishing the two GI clades (GI-a and GI-b), GI-b has shown a significantly wider and more rapid dispersal pattern in several regions in Asia than the GI-a clade, which remains restricted in its geographic distribution since its emergence...
October 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27703358/mir-370-mimic-inhibits-replication-of-japanese-encephalitis-virus-in-glioblastoma-cells
#13
Wenjuan Li, Peng Cheng, Shangdan Nie, Wen Cui
Japanese encephalitis (JE) is one of the most severe viral infections of the central nervous system. No effective treatment for JE currently exists, because its pathogenesis remains largely unknown. The present study was designed to screen the potential microRNAs (miRNAs) involved in JE. Glioblastoma cells were collected, after being infected with the Japanese encephalitis virus (JEV). Total miRNAs were extracted and analyzed using an miRNA chip. One of the most severely affected miRNAs was selected, and the role of miR-370 in JEV infection was investigated...
2016: Neuropsychiatric Disease and Treatment
https://www.readbyqxmd.com/read/27703031/quantitative-phosphoproteomic-analysis-identifies-the-critical-role-of-jnk1-in-neuroinflammation-induced-by-japanese-encephalitis-virus
#14
Jing Ye, Hao Zhang, Wen He, Bibo Zhu, Dengyuan Zhou, Zheng Chen, Usama Ashraf, Yanming Wei, Ziduo Liu, Zhen F Fu, Huanchun Chen, Shengbo Cao
Japanese encephalitis virus (JEV) is the leading cause of epidemic encephalitis worldwide. The pathogenesis of JEV is linked to a robust inflammatory response in the central nervous system (CNS). Glial cells are the resident immune cells in the CNS and represent critical effectors of CNS inflammation. To obtain a global overview of signaling events in glial cells during JEV infection, we conducted phosphoproteomics profiling of a JEV-infected glial cell line. We identified 1816 phosphopeptides, corresponding to 1264 proteins, that exhibited a change in phosphorylation status upon JEV infection...
October 4, 2016: Science Signaling
https://www.readbyqxmd.com/read/27702638/application-of-various-control-strategies-to-japanese-encephalitic-a-mathematical-study-with-human-pig-and-mosquito
#15
A De, K Maity, Soovoojeet Jana, M Maiti
Japanese encephalitis (JE) is a public health problem that threats the entire world today. Japanese Encephalitis virus (JEV) mostly became a threat due to the significant number of increase of susceptible mosquito vectors and vertebrate hosts in Asia by which around 70,000 cases and 10,000 deaths per year took place in children below 15 years of age. In this paper, a mathematical model of JE due to JEV from the vector source (infected mosquito) and two vertebrate hosts (infected human and infected pig) is formulated...
December 2016: Mathematical Biosciences
https://www.readbyqxmd.com/read/27697524/mouse-apolipoprotein-l9-is-a-phosphatidylethanolamine-binding-protein
#16
Thekkinghat Anantharaman Arvind, Pundi N Rangarajan
Mouse Apolipoprotein L9 (ApoL9) is an understudied cytoplasmic, interferon-inducible protein. The details of its intracellular localization and normal cellular functions are unclear. We report here that ApoL9 localizes to small puncta diffusely distributed in the cytoplasm, as well as to larger granules of varying size and number that are similar to aggresome-like induced structures (ALIS) and contain the autophagy receptor Sqstm1/p62, the autophagosome marker Lc3, and ubiquitin. Transfection of B16F10 mouse melanoma cells stably expressing ApoL9 (B16F10(L9)) with certain liposome-based transfection reagents causes dramatic disturbances in its subcellular distribution...
October 28, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27679979/structure-based-discovery-of-two-antiviral-inhibitors-targeting-the-ns3-helicase-of-japanese-encephalitis-virus
#17
Jin'e Fang, Huan Li, Dexin Kong, Shengbo Cao, Guiqing Peng, Rui Zhou, Huanchun Chen, Yunfeng Song
Japanese encephalitis virus (JEV) is a flavivirus that threatens more than half of the world's population. Vaccination can prevent the disease, but no specific antiviral drug is yet available for clinical therapy, and the death rate caused by JEV can reach as high as 60%. The C-terminus of non-structural protein 3 (NS3) of flavivirus encodes helicase and has been identified as a potential drug target. In this study, high throughput molecular docking was employed to identify candidate JEV NS3 helicase inhibitors in a commercial library containing 250,000 compounds...
September 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27667714/trim52-inhibits-japanese-encephalitis-virus-replication-by-degrading-the-viral-ns2a
#18
Wenchun Fan, Mengge Wu, Suhong Qian, Yun Zhou, Huanchun Chen, Xiangmin Li, Ping Qian
The members of tripartite-motif containing (TRIM) protein participate in various cellular processes and play an important role in host antiviral function. TRIM proteins exert their antiviral activity either directly by degrading viral proteins through their E3 ligase activity, or indirectly by promoting host innate immunity. This study demonstrated for the first time that TRIM52 is a novel antiviral TRIM protein against Japanese encephalitis virus (JEV) infection. Overexpression of TRIM52 restricted JEV replication in BHK-21 and 293T cells...
September 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27665292/a-simple-method-for-developing-an-infectious-cdna-clone-of-japanese-encephalitis-virus
#19
Hao Zheng, Xuchen Zheng, Wu Tong, Fei Liu, Chao Liang, Tao Wang, Fei Gao, Liwei Li, Tongling Shan, Guoxin Li, Guangzhi Tong
Flavivirus cDNA clones frequently demonstrate genetic instability in transformed bacteria, which hampers the construction and manipulation of cDNAs for infectious flaviviruses. In this study, we developed a stable, full-length cDNA clone, pJEHEN, of a GI JEV strain HEN0701 using a medium-copy-number pBR322 vector and propagating cDNA clones at room temperature. The virus vJEHEN recovered from the infectious clone was indistinguishable from the parent virus HEN0701 with respect to plaque morphology, growth kinetics, and virulence characteristics...
September 24, 2016: Virus Genes
https://www.readbyqxmd.com/read/27638121/serological-evidence-of-widespread-west-nile-virus-and-japanese-encephalitis-virus-infection-in-native-domestic-ducks-anas-platyrhynchos-var-domesticus-in-kuttanad-region-kerala-india
#20
Semmannan Kalaiyarasu, Niranjan Mishra, Rohit Kumar Khetan, Vijendra Pal Singh
Birds can act as reservoirs of West Nile virus (WNV) with a key role in its epidemiology. WNV lineage 1 associated fatal cases of human encephalitis in 2011 and acute flaccid paralysis in 2013 were reported in Alappuzha district, Kerala, India. But no information is available on WNV circulation in domestic ducks, which are abundant, cohabit with humans and occupy wetlands and water bodies in the region. To determine the extent of WNV infection, we investigated 209 sera, 250 oral and 350 cloacal swab samples from local Chara and Chemballi domestic ducks (Anas platyrhynchos var domesticus) in the districts of Alappuzha, Kottayam, Kollam and Pathanamthitta collected during January and March 2015...
October 2016: Comparative Immunology, Microbiology and Infectious Diseases
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