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https://www.readbyqxmd.com/read/28531165/a-japanese-encephalitis-virus-vaccine-inducing-antibodies-strongly-enhancing-in-vitro-infection-is-protective-in-pigs
#1
Obdulio García-Nicolás, Meret E Ricklin, Matthias Liniger, Nathalie J Vielle, Sylvie Python, Philippe Souque, Pierre Charneau, Artur Summerfield
The Japanese encephalitis virus (JEV) is responsible for zoonotic severe viral encephalitis transmitted by Culex mosquitoes. Although birds are reservoirs, pigs play a role as amplifying hosts, and are affected in particular through reproductive failure. Here, we show that a lentiviral JEV vector, expressing JEV prM and E proteins (TRIP/JEV.prME), but not JEV infection induces strong antibody-dependent enhancement (ADE) activities for infection of macrophages. Such antibodies strongly promoted infection via Fc receptors...
May 22, 2017: Viruses
https://www.readbyqxmd.com/read/28518046/epidemiologic-survey-of-japanese-encephalitis-virus-infection-tibet-china-2015
#2
Hui Zhang, Mujeeb Ur Rehman, Kun Li, Houqiang Luo, Yanfang Lan, Fazul Nabi, Lihong Zhang, Muhammad Kashif Iqbal, Suolangsi Zhu, Muhammad Tariq Javed, Yangzom Chamba, Jia Kui Li
We investigated Japanese encephalitis virus (JEV) prevalence in high-altitude regions of Tibet, China, by using standard assays to test mosquitoes, pigs, and humans. Results confirmed that JEV has spread to these areas. Disease prevention and control strategies should be used along with surveillance to limit spread of JEV in high-altitude regions of Tibet.
June 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28515719/production-of-japanese-encephalitis-virus-antigens-in-plants-using-bamboo-mosaic-virus-based-vector
#3
Tsung-Hsien Chen, Chung-Chi Hu, Jia-Teh Liao, Yi-Ling Lee, Ying-Wen Huang, Na-Sheng Lin, Yi-Ling Lin, Yau-Heiu Hsu
Japanese encephalitis virus (JEV) is among the major threats to public health in Asia. For disease control and prevention, the efficient production of safe and effective vaccines against JEV is in urgent need. In this study, we produced a plant-made JEV vaccine candidate using a chimeric virus particle (CVP) strategy based on bamboo mosaic virus (BaMV) for epitope presentation. The chimeric virus, designated BJ2A, was constructed by fusing JEV envelope protein domain III (EDIII) at the N-terminus of BaMV coat protein, with an insertion of the foot-and-mouth disease virus 2A peptide to facilitate the production of both unfused and epitope-presenting for efficient assembly of the CVP vaccine candidate...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28509619/japanese-encephalitis-virus-infection-rate-and-detection-of-genotype-i-from-culex-tritaeniorhynchus-collected-from-jiangsu-china
#4
Hongliang Chu, Zhiming Wu, Hongna Chen, Chunxiao Li, Xiaoxia Guo, Ran Liu, Gang Wang, Minghao Zhou, Tongyan Zhao
Information regarding the infection rate and genotype shifts for Japanese encephalitis virus (JEV) are important for JE vaccine application. In Jiangsu province, China, which is one of the provinces with a high prevalence of JE, JEV infection in swine and mosquitoes in certain cities has only been investigated in 2008-2009. Lianyungang City has one of the highest numbers of JE cases in Jiangsu province, and it has a high risk of JEV invasion via migrant birds. JEV infection in vectors in Lianyungang City, which has urban and rural parts, has not been investigated...
May 16, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28468311/tubacin-an-hdac6-selective-inhibitor-reduces-the-replication-of-the-japanese-encephalitis-virus-via-the-decrease-of-viral-rna-synthesis
#5
Chien-Yi Lu, Yi-Chih Chang, Chun-Hung Hua, Chieh Chuang, Su-Hua Huang, Szu-Hao Kung, Mann-Jen Hour, Cheng-Wen Lin
Japanese encephalitis virus (JEV), a neurotropic flavivirus, annually causes over 30,000 Japanese Encephalitis (JE) cases in East and Southeast Asia. Histone deacetylases (HDACs) modulate lysine acetylation of histones and non-histone proteins, regulating many processes including inflammation and antiviral immune response. This study investigated antiviral activity of pan- and selective-HDAC inhibitors as host-targeting agents against JEV. Among HDAC inhibitors, selective HDAC6 inhibitors (tubastatin-A (TBSA) and tubacin) concentration-dependently inhibited JEV-induced cytopathic effect and apoptosis, as well as reduced virus yield in human cerebellar medulloblastoma cells...
May 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28446752/near-atomic-structure-of-japanese-encephalitis-virus-reveals-critical-determinants-of-virulence-and-stability
#6
Xiangxi Wang, Shi-Hua Li, Ling Zhu, Qing-Gong Nian, Shuai Yuan, Qiang Gao, Zhongyu Hu, Qing Ye, Xiao-Feng Li, Dong-Yang Xie, Neil Shaw, Junzhi Wang, Thomas S Walter, Juha T Huiskonen, Elizabeth E Fry, Cheng-Feng Qin, David I Stuart, Zihe Rao
Although several different flaviviruses may cause encephalitis, Japanese encephalitis virus is the most significant, being responsible for thousands of deaths each year in Asia. The structural and molecular basis of this encephalitis is not fully understood. Here, we report the cryo-electron microscopy structure of mature Japanese encephalitis virus at near-atomic resolution, which reveals an unusual "hole" on the surface, surrounded by five encephalitic-specific motifs implicated in receptor binding. Glu138 of E, which is highly conserved in encephalitic flaviviruses, maps onto one of these motifs and is essential for binding to neuroblastoma cells, with the E138K mutation abrogating the neurovirulence and neuroinvasiveness of Japanese encephalitis virus in mice...
April 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/28443089/japanese-encephalitis-virus-exploits-dopamine-d2-receptor-phospholipase-c-to-target-dopaminergic-human-neuronal-cells
#7
Yogy Simanjuntak, Jian-Jong Liang, Yi-Ling Lee, Yi-Ling Lin
Despite the availability of vaccines for Japanese encephalitis virus (JEV), the re-emerging virus remains a clinically important pathogen that causes acute encephalitis and permanent neuropsychiatric sequels. JEV highly targets dopaminergic neuron-rich brain regions including the thalamus and midbrain. The molecular mechanism contributing to the high susceptibility of these particular brain regions remains largely unclear. This study addressed whether this tissue tropism of JEV is associated with signaling of dopaminergic neurons...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28441266/safety-of-the-inactivated-japanese-encephalitis-virus-vaccine-ixiaro%C3%A2-in-children-an-open-label-randomized-active-controlled-phase-3-study
#8
Katrin L Dubischar, Vera Kadlecek, Benjamin Sablan, Charissa Fay Borja-Tabora, Salvacion Gatchalian, Susanne Eder-Lingelbach, Zsuzsanna Mueller, Kerstin Westritschnig
BACKGROUND: Japanese encephalitis (JE) remains a serious health concern in Asian countries and has sporadically affected pediatric travelers. In the present study we monitored the safety profile of the JE virus (JEV) vaccine IXIARO in a pediatric population. METHODS: We randomized 1869 children between the age of 2 months and 17 years in an age-stratified manner to vaccination with IXIARO or one of the control vaccines, Prevnar and HAVRIX 720. Adverse events (AEs) (unsolicited and solicited local and systemic adverse events), serious AEs and medically-attended AEs were assessed up to Day 56 and Month 7 after the first dose...
April 24, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28435908/development-of-a-japanese-encephalitis-virus-like-particle-vaccine-in-silkworms-using-codon-optimised-prm-and-envelope-genes
#9
Sayaka Matsuda, Reiko Nerome, Kenichi Maegawa, Akira Kotaki, Shigeo Sugita, Kazunori Kawasaki, Kazumichi Kuroda, Ryoji Yamaguchi, Tomohiko Takasaki, Kuniaki Nerome
We have successfully prepared a Japanese encephalitis virus (JEV) - Nakayama virus like particle (NVLP) vaccine using synthetic codon-optimized prM and E genes. The expression of the recombinant JEV Nakayama-BmNPV (JEV-NNPV) virus was determined in infected silkworm Bm-N cells by fluorescence and Western blot analysis. The recombinant was inoculated into silkworm pupae and the yield of Nakayama VLP (NVLP) reached a peak in the homogenates after 3 days. Additionally, in the peptide analysis of infected pupae homogenate, it appeared approximately 300-500 μg E protein/pupa were produced...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28404855/genome-wide-screening-uncovers-the-significance-of-n-sulfation-of-heparan-sulfate-as-a-host-cell-factor-for-chikungunya-virus-infection
#10
Atsushi Tanaka, Uranan Tumkosit, Shota Nakamura, Daisuke Motooka, Natsuko Kishishita, Thongkoon Priengprom, Areerat Sa-Ngasang, Taroh Kinoshita, Naokazu Takeda, Yusuke Maeda
The molecular mechanisms underlying chikungunya virus (CHIKV) infection are poorly characterized. In this study, we analyzed the host factors involved in CHIKV infection using genome-wide screening. Human haploid HAP1 cells, into which an exon-trapping vector was introduced, were challenged with a vesicular stomatitis virus pseudotype bearing the CHIKV E3-E1 envelope proteins. Analysis of genes enriched in the cells resistant to the pseudotyped virus infection unveiled a critical role of N-sulfation of heparan sulfate (HS) for the infectivity of a clinically isolated CHIKV Thai #16856 strain to HAP1 cells...
April 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28394283/single-round-infectious-particle-antiviral-screening-assays-for-the-japanese-encephalitis-virus
#11
Chien-Yi Lu, Mann-Jen Hour, Ching-Ying Wang, Su-Hua Huang, Wen-Xiang Mu, Yu-Chun Chang, Cheng-Wen Lin
Japanese Encephalitis virus (JEV) is a mosquito-borne flavivirus with a positive-sense single-stranded RNA genome that contains a big open reading frame (ORF) flanked by 5'- and 3'- untranslated regions (UTRs). Nearly 30,000 JE cases with 10,000 deaths are still annually reported in East Asia. Although the JEV genotype III vaccine has been licensed, it elicits a lower protection against other genotypes. Moreover, no effective treatment for a JE case is developed. This study constructed a pBR322-based and cytomegaloviruses (CMV) promoter-driven JEV replicon for the production of JEV single-round infectious particles (SRIPs) in a packaging cell line expressing viral structural proteins...
April 10, 2017: Viruses
https://www.readbyqxmd.com/read/28382163/visualization-of-a-neurotropic-flavivirus-infection-in-mouse-reveals-unique-viscerotropism-controlled-by-host-type-i-interferon-signaling
#12
Xiao-Feng Li, Xiao-Dan Li, Cheng-Lin Deng, Hao-Long Dong, Qiu-Yan Zhang, Qing Ye, Han-Qing Ye, Xing-Yao Huang, Yong-Qiang Deng, Bo Zhang, Cheng-Feng Qin
Flavivirus includes a large group of human pathogens with medical importance. Especially, neurotropic flaviviruses capable of invading central and peripheral nervous system, e.g. Japanese encephalitis virus (JEV) and Zika virus (ZIKV), are highly pathogenic to human and constitute major global health problems. However, the dynamic dissemination and pathogenesis of neurotropic flavivirus infections remain largely unknown. Here, using JEV as a model, we rationally designed and constructed a recombinant reporter virus that stably expressed Renilla luciferase (Rluc)...
2017: Theranostics
https://www.readbyqxmd.com/read/28352048/evidence-of-jev-in-culex-tritaeniorhynchus-and-pigs-from-high-altitude-regions-of-tibet-china
#13
Hui Zhang, Houqiang Luo, Mujeeb Ur Rehman, Fazul Nabi, Kun Li, Yanfang Lan, Shucheng Huang, Lihong Zhang, Khalid Mehmood, Muhammad Shahzad, Jiakui Li
BACKGROUND & OBJECTIVES: Culex tritaeniorhynchus is the primary vector of Japanese encephalitis virus (JEV) which is a major threat to animals and humans health. This virus has been reported earlier from low altitude regions of Tibet, in mosquitoes, Tibetan pigs and local Tibetans, but no reports are available for the probable propagation of JE to high altitude regions (3100 m) of Tibet. This study aimed to find the evidence of JEV in Cx. tritaeniorhynchus and pigs from high altitude regions of Tibet, China...
January 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28349961/e3-ubiquitin-ligase-nedd4-promotes-japanese-encephalitis-virus-replication-by-suppressing-autophagy-in-human-neuroblastoma-cells
#14
Qingqiang Xu, Naiwei Zhu, Shenglin Chen, Ping Zhao, Hao Ren, Shiying Zhu, Hailin Tang, Yongzhe Zhu, Zhongtian Qi
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes the most prevalent viral encephalitis in Asia. Since JEV is a neurotropic virus, it is important to identify key molecules that mediate JEV infection in neuronal cells and to investigate their underlying mechanisms. In this study, the critical role of Nedd4, an E3 ubiquitin ligase that is highly expressed in the central nervous system, was examined in JEV propagation. In SK-N-SH neuroblastoma cells, Nedd4 was up-regulated in response to JEV infection...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28301270/japanese-encephalitis-vaccines-immunogenicity-protective-efficacy-effectiveness-and-impact-on-the-burden-of-disease
#15
Nagendra R Hegde, Milind M Gore
Japanese encephalitis (JE) is a serious public health concern in most of Asia. The disease is caused by JE virus (JEV), a flavivirus transmitted by Culex mosquitoes. Several vaccines have been developed to control JE in endemic areas as well as to protect travelers and military personnel who visit or are commissioned from non-endemic to endemic areas. The vaccines include inactivated vaccines produced in mouse brain or cell cultures, live attenuated vaccines, and a chimeric vaccine based on the live attenuated yellow fever virus 17D vaccine strain...
February 22, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28293227/mlkl-mediated-necroptosis-accelerates-jev-induced-neuroinflammation-in-mice
#16
Peiyu Bian, Xuyang Zheng, Li Wei, Chuantao Ye, Hong Fan, Yanhui Cai, Ying Zhang, Fanglin Zhang, Zhansheng Jia, Yingfeng Lei
Japanese encephalitis virus (JEV) is the most prevalent cause of viral encephalitis in Asia and the western Pacific. Neuronal death caused by JEV infection and inflammation induced cytotoxicity leads to progression and deterioration of Japanese encephalitis (JE). Mixed-lineage kinase domain-like protein (MLKL) mediated necroptosis is a newly discovered pathway of programmed cell death and participates in many inflammatory diseases. In this study, we demonstrated for the first time that necroptosis was involved in the neuronal loss during JE via immune-electron microscopy and immunochemistry...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28284628/japanese-encephalitis-virus-infection-diagnosis-and-control-in-domestic-animals
#17
REVIEW
Karen L Mansfield, Luis M Hernández-Triana, Ashley C Banyard, Anthony R Fooks, Nicholas Johnson
Japanese encephalitis virus (JEV) is a significant cause of neurological disease in humans throughout Asia causing an estimated 70,000 human cases each year with approximately 10,000 fatalities. The virus contains a positive sense RNA genome within a host-derived membrane and is classified within the family Flaviviridae. Like many flaviviruses, it is transmitted by mosquitoes, particularly those of the genus Culex in a natural cycle involving birds and some livestock species. Spill-over into domestic animals results in a spectrum of disease ranging from asymptomatic infection in some species to acute neurological signs in others...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28279803/japanese-encephalitis-virus-disrupts-blood-brain-barrier-and-modulates-apoptosis-proteins-in-thbmec-cells
#18
Mazen M Jamil Al-Obaidi, Azadeh Bahadoran, Lee Sau Har, Wang Seok Mui, Jayakumar Rajarajeswaran, Keivan Zandi, Rishya Manikam, Shamala Devi Sekaran
Japanese encephalitis (JE) is a neurotropic flavivirus that causes inflammation in central nervous system (CNS), neuronal death and also compromises the structural and functional integrity of the blood-brain barrier (BBB). The aim of this study was to evaluate the BBB disruption and apoptotic process in Japanese encephalitis virus (JEV)-infected transfected human brain microvascular endothelial cells (THBMECs). THBMECs were overlaid by JEV with different MOIs (0.5, 1.0, 5.0 and 10.0) and monitored by electrical cell-substrate impedance sensing (ECIS) in a real-time manner in order to observe the barrier function of THBMECs...
March 6, 2017: Virus Research
https://www.readbyqxmd.com/read/28265274/tlr7-deficiency-leads-to-tlr8-compensative-regulation-of-immune-response-against-jev-in-mice
#19
Muhammad Awais, Ke Wang, Xianwu Lin, Wenjie Qian, Nan Zhang, Chong Wang, Kunlun Wang, Ling Zhao, Zhen F Fu, Min Cui
Japanese encephalitis virus (JEV) is a highly fatal pathogen to human beings. Toll-like receptor 7 (TLR7) plays a role as the first host defense against most single-stranded RNA flaviviruses. This study aims to investigate the role of TLR7 in inducing adaptive immune response in mice against JEV. In vitro and in vivo studies were conducted to examine the expression of toll-like receptors (TLRs) in mice. After JEV infection, physical parameters of mice (survival rate and body weight) were evaluated, and organs or cells were collected for further analysis...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28264652/insight-into-snps-and-epitopes-of-e-protein-of-newly-emerged-genotype-i-isolates-of-jev-from-midnapur-west-bengal-india
#20
Shyamashree Banerjee, Parth Sarthi Sen Gupta, Amal Kumar Bandyopadhyay
BACKGROUND: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes Japanese Encephalitis (JE) and Acute Encephalitis Syndrome (AES) in humans. Genotype-I (as co-circulating cases with Genotype-III) was isolated in 2010 (JEV28, JEV21) and then in 2011 (JEV45) from Midnapur district, West Bengal (WB) for the first time from clinical patients who were previously been vaccinated with live attenuated SA14-14-2 strain. We apply bioinformatics and immunoinformatics on sequence and structure of E protein for analysis of crucial substitutions that might cause the genotypic transition, affecting protein-function and altering specificity of epitopes...
March 6, 2017: BMC Immunology
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