Liam K R Sharkey, Romain Guerillot, Calum J Walsh, Adrianna M Turner, Jean Y H Lee, Stephanie L Neville, Stephan Klatt, Sarah L Baines, Sacha J Pidot, Fernando J Rossello, Torsten Seemann, Hamish E G McWilliam, Ellie Cho, Glen P Carter, Benjamin P Howden, Christopher A McDevitt, Abderrahman Hachani, Timothy P Stinear, Ian R Monk
Among the 16 two-component systems in the opportunistic human pathogen Staphylococcus aureus , only WalKR is essential. Like the orthologous systems in other Bacillota, S. aureus WalKR controls autolysins involved in peptidoglycan remodeling and is therefore intimately involved in cell division. However, despite the importance of WalKR in S. aureus , the basis for its essentiality is not understood and the regulon is poorly defined. Here, we defined a consensus WalR DNA-binding motif and the direct WalKR regulon by using functional genomics, including chromatin immunoprecipitation sequencing, with a panel of isogenic walKR mutants that had a spectrum of altered activities...
October 16, 2023: MBio