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Auttawit Sirichoat, Aroonlug Lulitanond, Rattiyaporn Kanlaya, Ratree Tavichakorntrakool, Aroonwadee Chanawong, Sujintana Wongthong, Visith Thongboonkerd
Reduced vancomycin susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide problem. Unfortunately, its genetic marker and molecular mechanisms remained unknown. This study investigated differential phenotypic characteristic and protein expression profiles among three groups of MRSA isolates, including vancomycin-susceptible S. aureus (VSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) (n = 7 isolates/group). Phenotypic characteristic revealed significant greater number of isolates with non-spreading colony in VISA as compared to both VSSA and hVISA groups...
September 17, 2016: Diagnostic Microbiology and Infectious Disease
Inayat Ur Rahman, Farhana Ijaz, Aftab Afzal, Zafar Iqbal, Niaz Ali, Shujaul Mulk Khan
ETHNO PHARMACOLOGICAL RELEVANCE: Ethno medicinal traditional knowledge regarding the uses of indigenous medicinal plants used for various human digestive disorders are mostly known to the elder community members. As the young generation is not much aware about such vital traditional medicinal practice because they rely on elders. AIM OF STUDY: To document, accumulate and widely disseminate the massive indigenous knowledge of century's practiced therapeutic uses of medicinal plants by the local people living in this remote area...
June 25, 2016: Journal of Ethnopharmacology
E N Anyabolu, I I Chukwuonye, E Arodiwe, C K Ijoma, I Ulasi
Human immunodeficiency virus (HIV) infection is a common cause of chronic kidney disease (CKD) in Sub-Saharan Africa. This study aims at identifying the prevalence and predictors of CKD in newly diagnosed HIV patients in Owerri, South East Nigeria. This was a cross-sectional study consisting of 393 newly diagnosed HIV-seropositive subjects and 136 age- and sex-matched seronegative subjects as controls. CKD was defined as 24-hour urine protein (24-HUP) ≥0.3 g and/or glomerular filtration rate (GFR) < 60 ml/min...
January 2016: Indian Journal of Nephrology
Sachiko Masuda, Masaki Saito, Chiaki Sugawara, Manabu Itakura, Shima Eda, Kiwamu Minamisawa
The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H2 released by nitrogenase in soybean nodule symbiosis, and is responsible for H2-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H2-dependent chemolithoautotrophic conditions was markedly weaker and H2 production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS1L1) than for the mutant of hup locus II (ΔhupS2L2)...
2016: Microbes and Environments
Hyun Ho Ryu, Johanna C Moore, Demetris Yannopoulos, Michael Lick, Scott McKnite, Sang Do Shin, Tae Yun Kim, Anja Metzger, Jennifer Rees, Adamantios Tsangaris, Guillaume Debaty, Keith G Lurie
AIM: Chest compressions during cardiopulmonary resuscitation (CPR) increase arterial and venous pressures, delivering simultaneous bidirectional high-pressure compression waves to the brain. We hypothesized that this may be detrimental and could be partially overcome by elevation of the head during CPR. MEASUREMENTS: Female Yorkshire farm pigs (n=30) were sedated, intubated, anesthetized, and placed on a table able to elevate the head 30° (15cm) (HUP) and the heart 10° (4cm) or remain in the supine (SUP) flat position during CPR...
May 2016: Resuscitation
Ildikó K Nagy, Kornél L Kovács, Gábor Rákhely, Gergely Maróti
[NiFe]-hydrogenases are regulated by various factors to fulfill their physiological functions in bacterial cells. The photosynthetic purple sulfur bacterium Thiocapsa roseopersicina harbors four functional [NiFe]-hydrogenases: HynSL, HupSL, Hox1, and Hox2. Most of these hydrogenases are functionally linked to sulfur metabolism, and thiosulfate has a central role in this organism. The membrane-associated Hup hydrogenases have been shown to play a role in energy conservation through hydrogen recycling. The expression of Hup-type hydrogenases is regulated by H2 in Rhodobacter capsulatus and Cupriavidus necator; however, it has been shown that the corresponding hydrogen-sensing system is nonfunctional in T...
April 2016: Applied and Environmental Microbiology
Holly Geyer, Robyn Scherber, Heidi Kosiorek, Amylou C Dueck, Jean-Jacques Kiladjian, Zhijian Xiao, Stefanie Slot, Sonja Zweegman, Federico Sackmann, Ana Kerguelen Fuentes, Dolores Hernández-Maraver, Konstanze Döhner, Claire N Harrison, Deepti Radia, Pablo Muxi, Carlos Besses, Francisco Cervantes, Peter L Johansson, Bjorn Andreasson, Alessandro Rambaldi, Tiziano Barbui, Karin Bonatz, Andreas Reiter, Francoise Boyer, Gabriel Etienne, Jean-Christophe Ianotto, Dana Ranta, Lydia Roy, Jean-Yves Cahn, Norman Maldonado, Giovanni Barosi, Maria L Ferrari, Robert Peter Gale, Gunnar Birgegard, Zefeng Xu, Yue Zhang, Xiujuan Sun, Junqing Xu, Peihong Zhang, Peter A W te Boekhorst, Suzan Commandeur, Harry Schouten, Heike L Pahl, Martin Griesshammer, Frank Stegelmann, Thomas Lehmann, Zhenya Senyak, Alessandro M Vannucchi, Francesco Passamonti, Jan Samuelsson, Ruben A Mesa
PURPOSE: Polycythemia vera (PV) is a myeloproliferative neoplasm (MPN) associated with disabling symptoms and a heightened risk of life-threatening complications. Recent studies have demonstrated the effectiveness of JAK inhibitor therapy in patients with PV patients who have a history of prior hydroxyurea (HU) use (including resistance or intolerance), phlebotomy requirements, and palpable splenomegaly. We aimed to determine how these features contribute alone and in aggregate to the PV symptom burden...
January 10, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Adolfo Flores Saiffe Farías, Enrique Jaime Herrera López, Cristopher Jorge Moreno Vázquez, Wentian Li, Ernesto Prado Montes de Oca
Single nucleotide polymorphisms (SNPs) in transcription factor binding sites (TFBSs) within gene promoter region or enhancers can modify the transcription rate of genes related to complex diseases. These SNPs can be called regulatory SNPs (rSNPs). Data compiled from recent projects, such as the 1000 Genomes Project and ENCODE, has revealed essential information used to perform in silico prediction of the molecular and biological repercussions of SNPs within TFBS. However, most of these studies are very limited, as they only analyze SNPs in coding regions or when applied to promoters, and do not integrate essential biological data like TFBSs, expression profiles, pathway analysis, homotypic redundancy (number of TFBSs for the same TF in a region), chromatin accessibility and others, which could lead to a more accurate prediction...
December 2015: Computational Biology and Chemistry
Zhi Dong Zhou, Shao Ping Xie, Sushmitha Sathiyamoorthy, Wuan Ting Saw, Tan Ye Sing, Shin Hui Ng, Heidi Pek Hup Chua, Alyssa Mei Yan Tang, Fathima Shaffra, Zeng Li, Hongyan Wang, Patrick Ghim Hoe Ho, Mitchell Kim Peng Lai, Dario C Angeles, Tit Meng Lim, Eng-King Tan
The mutations of F-box protein 7 (FBXO7) gene (T22M, R378G and R498X) are associated with a severe form of autosomal recessive juvenile-onset Parkinson's disease (PD) (PARK 15). Here we demonstrated that wild-type (WT) FBXO7 is a stress response protein and it can play both cytoprotective and neurotoxic roles. The WT FBXO7 protein is vital to cell mitophagy and can facilitate mitophagy to protect cells, whereas mutant FBXO7 inhibits mitophagy. Upon stress, the endogenous WT FBXO7 gets up-regulated, concentrates into mitochondria and forms FBXO7 aggregates in mitochondria...
November 15, 2015: Human Molecular Genetics
Basar Uyar, Muazzez Gürgan, Ebru Özgür, Ufuk Gündüz, Meral Yücel, Inci Eroglu
Photofermentative production of hydrogen is a promising and sustainable process; however, it should be coupled to dark fermentation to become cost effective. In order to integrate dark fermentation and photofermentation, the suitability of dark fermenter effluents for the photofermentative hydrogen production must be demonstrated. In this study, thermophilic dark fermenter effluent (DFE) of sugar beet thick juice was used as a substrate in photofermentation process to compare wild-type and uptake hydrogenase-deficient (hup (-)) mutant strains of Rhodobacter capsulatus by means of hydrogen production and biomass growth...
October 2015: Bioprocess and Biosystems Engineering
K Szegedi, R Lutter, P C Res, J D Bos, R M Luiten, S Kezic, M A Middelkamp-Hup
BACKGROUND: The in vivo levels of inflammatory mediators in chronic atopic dermatitis (AD) skin are not well-defined due to the lack of a non-invasive or minimally invasive sampling technique. OBJECTIVES: To investigate the cytokine milieu in interstitial fluid (ISF) collected from chronic lesional AD skin as compared to ISF from non-lesional AD skin and/or healthy donor skin. METHODS: ISF was obtained using a minimally invasive technique of creating micropores in the skin by a laser, and harvesting ISF through aspiration...
November 2015: Journal of the European Academy of Dermatology and Venereology: JEADV
Patrícia Raleiras, Leif Hammarström, Peter Lindblad, Stenbjörn Styring, Ann Magnuson
The small subunit from the NiFe uptake hydrogenase, HupSL, in the cyanobacterium Nostoc punctiforme ATCC 29133, has been isolated in the absence of the large subunit (P. Raleiras, P. Kellers, P. Lindblad, S. Styring, A. Magnuson, J. Biol. Chem. 288 (2013) 18,345-18,352). Here, we have used flash photolysis to reduce the iron-sulfur clusters in the isolated small subunit, HupS. We used ascorbate as electron donor to the photogenerated excited state of Ru(II)-trisbipyridine (Ru(bpy)3), to generate Ru(I)(bpy)3 as reducing agent...
July 2015: Journal of Inorganic Biochemistry
Nurul I Hariadi, Lixin Zhang, Mayuri Patel, Sara A Sandstedt, Gregg S Davis, Carl F Marrs, Janet R Gilsdorf
Nontypeable Haemophilus influenzae (NTHI) are Gram-negative bacteria that colonize the human pharynx and can cause respiratory tract infections, such as acute otitis media (AOM). Since NTHI require iron from their hosts for aerobic growth, the heme acquisition genes may play a significant role in avoiding host nutritional immunity and determining virulence. Therefore, we employed a hybridization-based technique to compare the prevalence of five heme acquisition genes (hxuA, hxuB, hxuC, hemR, and hup) between 514 middle ear strains from children with AOM and 235 throat strains from healthy children...
July 2015: Journal of Clinical Microbiology
Sara H G Sinclair, Jose C Garcia-Garcia, J Stephen Dumler
Obligate intracellular bacteria have an arsenal of proteins that alter host cells to establish and maintain a hospitable environment for replication. Anaplasma phagocytophilum secrets Ankyrin A (AnkA), via a type IV secretion system, which translocates to the nucleus of its host cell, human neutrophils. A. phagocytophilum-infected neutrophils have dramatically altered phenotypes in part explained by AnkA-induced transcriptional alterations. However, it is unlikely that AnkA is the sole effector to account for infection-induced transcriptional changes...
2015: Frontiers in Microbiology
Nirag Jhala, Prabodh K Gupta
This document briefly captures the development of cytopathology at the Hospital of the University of Pennsylvania (HUP) in Philadelphia PA; the first medical school and the teaching hospital in the Country. Literature suggests that cells from malignancy have been described since early 1830s. While earlier accounts are not available, in the year 1895, 9th edition of the book written by Professor of Pathology, Dr. Tyson of the University of Pennsylvania describes urothelial cell morphology. It is also noted that both gynecologic as well as non-gynecologic cytopathology is routinely being practiced at this Institution since 1949...
February 2015: Diagnostic Cytopathology
Oscar Almarza, Daniel Núñez, Hector Toledo
BACKGROUND: Bacterial genomes are compacted by association with histone-like proteins to form a complex known as bacterial chromatin. The histone-like protein HU is capable of binding and bending the DNA molecule, a function related to compaction, protection, and regulation of gene expression. In Helicobacter pylori, HU is the only histone-like protein described so far. Proteomic analysis from our laboratory showed that this protein is overexpressed under acidic stress. MATERIALS AND METHODS: We used a purified recombinant wild-type protein and two mutant proteins with the amino acid substitutions K3A/S27D and K62R/V63N/P64A to characterize the function of the N-terminal domain and the flexible arm of HU...
February 2015: Helicobacter
Layla K Mahdi, Tahereh Deihimi, Fatemeh Zamansani, Mario Fruzangohar, David L Adelson, James C Paton, Abiodun D Ogunniyi, Esmaeil Ebrahimie
BACKGROUND: Streptococcus pneumoniae (the pneumococcus) is the world's foremost microbial pathogen, killing more people each year than HIV, TB or malaria. The capacity to penetrate deeper host tissues contributes substantially to the ability of this organism to cause disease. Here we investigated, for the first time, functional genomics modulation of 3 pneumococcal strains (serotype 2 [D39], serotype 4 [WCH43] and serotype 6A [WCH16]) during transition from the nasopharynx to lungs to blood and to brain of mice at both promoter and domain activation levels...
2014: BMC Genomics
Cresten B Mansfeldt, Annette R Rowe, Gretchen L W Heavner, Stephen H Zinder, Ruth E Richardson
A cDNA-microarray was designed and used to monitor the transcriptomic profile of Dehalococcoides mccartyi strain 195 (in a mixed community) respiring various chlorinated organics, including chloroethenes and 2,3-dichlorophenol. The cultures were continuously fed in order to establish steady-state respiration rates and substrate levels. The organization of array data into a clustered heat map revealed two major experimental partitions. This partitioning in the data set was further explored through principal component analysis...
October 2014: Applied and Environmental Microbiology
Daiana Renck, André A Santos, Pablo Machado, Guilherme O Petersen, Tiago G Lopes, Diógenes S Santos, Maria M Campos, Luiz A Basso
PURPOSE: 5-fluorouracil (5-FU) has been broadly used to treat solid tumors for more than 50 years. One of the major side effects of fluoropyrimidines therapy is oral and intestinal mucositis. Human uridine phosphorylase (hUP) inhibitors have been suggested as modulators of 5-FU toxicity. Therefore, the present study aimed to test the ability of hUP blockers in preventing mucositis induced by 5-FU. METHODS: We induced intestinal mucositis in Wistar rats with 5-FU, and the intestinal damage was evaluated in presence or absence of two hUP1 inhibitors previously characterized...
December 2014: Investigational New Drugs
Chunfang Gao, Yun Wang, Yue Shen, Dong Yan, Xi He, Junbiao Dai, Qingyu Wu
BACKGROUND: Microalgae-derived biodiesel is a promising substitute for conventional fossil fuels. In particular, the green alga Chlorella protothecoides sp. 0710 is regarded as one of the best candidates for commercial manufacture of microalgae-derived biofuel. This is due not only to its ability to live autotrophically through photosynthesis, but also to its capacity to produce a large amount of biomass and lipid through fermentation of glucose. However, until the present study, neither its genome sequence nor the platform required for molecular manipulations were available...
2014: BMC Genomics
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