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https://www.readbyqxmd.com/read/28738639/a-colorimetric-indicator-displacement-assay-for-cysteine-sensing-based-on-a-molecule-exchange-mechanism
#1
Zhonghua Xue, Xiaoxia Fu, Honghong Rao, Mohammed Hassan Ibrahim, Lulu Xiong, Xiuhui Liu, Xiaoquan Lu
In this study, we developed an ingenious yet effective strategy for cysteine detection. The colorimetric cysteine assay is established through an indicator displacement process, where Cu(2+) and pyrocatechol violet (PV) was employed as receptor and indicator, respectively. Proof-of-concept trials demonstrated that the stronger binding affinity of Cu(2+) receptor toward cysteine than PV indicator endowed our colorimetric sensor with high selectivity and excellent sensitivity as well as with a lower detection limit (4...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738638/mixed-matrix-membranes-incorporated-with-ln-mof-for-selective-and-sensitive-detection-of-nitrofuran-antibiotics-based-on-inner-filter-effect
#2
Feng Zhang, Hua Yao, Yifan Zhao, Xing Li, Gaowei Zhang, Yangyi Yang
The detection of antibiotics is critical and challenging due to the pervasive use of antibiotics had inevitably brought some negative impacts on ecosystem and human health. Herein, we reported an anti-interfere ability enhanced luminescent sensor of lanthanide metal-organic framework (Ln-MOF) filled mixed matrix membranes (MMMs), which combining the processibility of poly(methyl methacrylate) (PMMA) polymer with Ln-MOF of {[Tb2(AIP)2(H2O)10]·(AIP)·4H2O}n (Tb-AIP, where AIP is 5-aminoisophthalate) fillers...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738602/portable-electrochemical-system-using-screen-printed-electrodes-for-monitoring-corrosion-inhibitors
#3
André L Squissato, Weberson P Silva, Augusto T S Del Claro, Diego P Rocha, Rafael M Dornellas, Eduardo M Richter, Christopher W Foster, Craig E Banks, Rodrigo A A Munoz
This work presents a portable electrochemical system for the continuous monitoring of corrosion inhibitors in a wide range of matrices including ethanol, seawater and mineral oil following simple dilution of the samples. Proof-of-concept is demonstrated for the sensing of 2,5-dimercapto-1,3,5-thiadiazole (DMCT), an important corrosion inhibitor. Disposable screen-printed graphitic electrodes (SPGEs) associated with a portable batch-injection cell are proposed for the amperometric determination of DMCT following sample dilution with electrolyte (95% v/v ethanol + 5% v/v 0...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738593/boron-nitride-nanosheets-as-a-platform-for-fluorescence-sensing
#4
Yuanjin Zhan, Jingjing Yan, Mei Wu, Longhua Guo, Zhenyu Lin, Bin Qiu, Guonan Chen, Kwok-Yin Wong
Hexagonal boron nitride nanosheets (BNNS), one kind of inorganic graphene analogue, exhibit high fluorescence quenching ability via the photo-induced electron transfer (PET) and different affinity toward single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). As a proof of concept, the BNNS was first used as a sensing platform for the rapid detection of DNA and small molecules with high sensitivity and selectivity in this study. This strategy is versatile and quick fluorescence sensing of DNA and extensive DNA related analytes such as metal cations and small molecules...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738580/true-lab-in-a-syringe-technology-for-bioassays
#5
Lenka Hárendarčíková, Daniel Baron, Andrea Šebestová, Jan Rozsypal, Jan Petr
In our work, we introduced a novel concept of the lab-in-a-syringe tests. We solved the problem of detection in already published LIS tests by putting all the reaction and detection pads directly into the syringe barrel. We also used more layers to make the results visible for users. Two detection layouts: (i) with using rounded pads-based detection, and (ii) with using rectangular detection pads, were studied. As the proof of concept, we studied the determination of Ni(II) using dimethylglyoxime as the reagent and blocking of the interference of Fe(II)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738565/zwitterionic-hydrophilic-interaction-liquid-chromatography-tandem-mass-spectrometry-with-hybridspe-precipitation-for-the-determination-of-intact-cisplatin-in-human-plasma
#6
Feifan Xie, Pieter Colin, Jan Van Bocxlaer
Cisplatin is a first-line chemotherapeutic for the treatment of a wide variety of cancers since its discovery in the 1960s. Although various techniques have been reported for the measurement of total platinum in biological matrices, such as inductively coupled plasma-mass spectrometry and derivatization procedures, a specific, sensitive and robust assay for the quantification of intact cisplatin is still lacking. Therefore, we present a rapid, selective, sensitive, and reliable UHPLC-MS/MS based method for the determination of intact cisplatin in human plasma in support of a Phase II clinical trial...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28737887/enhanced-triboelectric-nanogenerators-based-on-mos2-monolayer-nanocomposites-acting-as-electron-acceptor-layers
#7
Chaoxing Wu, Tae Whan Kim, Jae Hyeon Park, Haoqun An, Jiajia Shao, Xiangyu Chen, Zhong Lin Wang
As one of their major goals, researchers attempting to harvest mechanical energy efficiently have continuously sought ways to integrate mature technologies with cutting-edge designs to enhance the performances of triboelectric nanogenerators (TENGs). In this research, we introduced monolayer molybdenum-disulfide (MoS2) into the friction layer of a TENG as the triboelectric electron-acceptor layer in an attempt to dramatically enhance its output performance. As a proof of the concept, we fabricated a vertical contact-separation mode TENG containing monolayer MoS2 as an electron-acceptor layer and found that the TENG exhibited a peak power density as large as 25...
July 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28737512/thrombin-promotes-diet-induced-obesity-through-fibrin-driven-inflammation
#8
Anna K Kopec, Sara R Abrahams, Sherry Thornton, Joseph S Palumbo, Eric S Mullins, Senad Divanovic, Hartmut Weiler, A Phillip Owens, Nigel Mackman, Ashley Goss, Joanne van Ryn, James P Luyendyk, Matthew J Flick
Obesity promotes a chronic inflammatory and hypercoagulable state that drives cardiovascular disease, type 2 diabetes, fatty liver disease, and several cancers. Elevated thrombin activity underlies obesity-linked thromboembolic events, but the mechanistic links between the thrombin/fibrin(ogen) axis and obesity-associated pathologies are incompletely understood. In this work, immunohistochemical studies identified extravascular fibrin deposits within white adipose tissue and liver as distinct features of mice fed a high-fat diet (HFD) as well as obese patients...
July 24, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28737272/dynamic-coupling-between-fmri-local-connectivity-and-interictal-eeg-in-focal-epilepsy-a-wavelet-analysis-approach
#9
Amir Omidvarnia, Mangor Pedersen, David N Vaughan, Jennifer M Walz, David F Abbott, Andrew Zalesky, Graeme D Jackson
Simultaneous scalp EEG-fMRI recording is a noninvasive neuroimaging technique for combining electrophysiological and hemodynamic aspects of brain function. Despite the time-varying nature of both measurements, their relationship is usually considered as time-invariant. The aim of this study was to detect direct associations between scalp-recorded EEG and regional changes of hemodynamic brain connectivity in focal epilepsy through a time-frequency paradigm. To do so, we developed a voxel-wise framework that analyses wavelet coherence between dynamic regional phase synchrony (DRePS, calculated from fMRI) and band amplitude fluctuation (BAF) of a target EEG electrode with dominant interictal epileptiform discharges (IEDs)...
July 24, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28737174/inverted-battery-design-as-ion-generator-for-interfacing-with-biosystems
#10
Chengwei Wang, Kun Kelvin Fu, Jiaqi Dai, Steven D Lacey, Yonggang Yao, Glenn Pastel, Lisha Xu, Jianhua Zhang, Liangbing Hu
In a lithium-ion battery, electrons are released from the anode and go through an external electronic circuit to power devices, while ions simultaneously transfer through internal ionic media to meet with electrons at the cathode. Inspired by the fundamental electrochemistry of the lithium-ion battery, we envision a cell that can generate a current of ions instead of electrons, so that ions can be used for potential applications in biosystems. Based on this concept, we report an 'electron battery' configuration in which ions travel through an external circuit to interact with the intended biosystem whereas electrons are transported internally...
July 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28736959/in-vivo-raman-spectroscopy-assisted-early-identification-of-potential-second-primary-recurrences-in-oral-cancers-an-exploratory-study
#11
Akshat Malik, Aditi Sahu, S P Singh, Atul Deshmukh, Pankaj Chaturvedi, Deepa Nair, Sudhir Nair, C Murali Krishna
BACKGROUND: Higher rates of local recurrences and second primaries, ascribable to field cancerization, are known problem in oral cancers. The present study explored utility of identification of potential recurrences by Raman spectroscopy, which has been shown to identify oral precancers, cancers, and field cancerization in humans and micro-sized mechanical irritation-induced tumors in animals. METHODS: Raman spectra were acquired from tumor and contralateral normal mucosa in 99 patients with oral cancer who were then followed up for appearance of clinically apparent cancerous lesions...
July 24, 2017: Head & Neck
https://www.readbyqxmd.com/read/28735873/the-therapeutic-potential-of-purinergic-signalling
#12
REVIEW
Geoffrey Burnstock
This review is focused on the pathophysiology and therapeutic potential of purinergic signalling. A wide range of diseases are considered, including those of the central nervous system, skin, kidney, musculoskeletal, liver gut, lower urinary tract, cardiovascular, airways and reproductive systems, the special senses, infection, diabetes and obesity. Several purinergic drugs are already on the market, including P2Y12 receptor antagonists for stroke and thrombosis, P2Y2 receptor agonists for dry eye, A1 receptor agonists for supraventricular tachycardia...
July 20, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28735755/protective-effects-of-acute-exercise-prior-to-doxorubicin-on-cardiac-function-of-breast-cancer-patients-a-proof-of-concept-rct
#13
A A Kirkham, R E Shave, K A Bland, J M Bovard, N D Eves, K A Gelmon, D C McKenzie, S A Virani, E J Stöhr, D E R Warburton, K L Campbell
BACKGROUND: Preclinical studies have reported that a single treadmill session performed 24h prior to doxorubicin provides cardio-protection. We aimed to characterize the acute change in cardiac function following an initial doxorubicin treatment in humans and determine whether an exercise session performed 24h prior to treatment changes this response. METHODS: Breast cancer patients were randomized to either 30min of vigorous-intensity exercise 24h prior to the first doxorubicin treatment (n=13), or no vigorous exercise for 72h prior to treatment (control, n=11)...
July 14, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28735028/protein-only-antimicrobial-peptide-containing-recombinant-nanoparticles-with-inherent-built-in-antibacterial-activity
#14
Naroa Serna, Laura Sánchez-García, Alejandro Sánchez-Chardi, Ugutz Unzueta, Mónica Roldán, Ramón Mangues, Esther Vázquez, Antonio Villaverde
The emergence of bacterial antibiotic resistances is a serious concern in human and animal health. In this context, naturally occurring cationic antimicrobial peptides (AMPs) might play a main role in a next generation of drugs against bacterial infections. Taking an innovative approach to design self-organizing functional proteins, we have generated here protein-only nanoparticles with intrinsic AMP microbicide activity. Using a recombinant version of the GWH1 antimicrobial peptide as building block, these materials show a wide antibacterial activity spectrum in absence of detectable toxicity on mammalian cells...
July 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28733851/autism-and-equine-assisted-interventions-a-systematic-mapping-review
#15
B Caitlin McDaniel Peters, Wendy Wood
This systematic mapping review mapped current knowledge of equine-assisted interventions for people with autism to help guide future practice and research. Thirty-three studies including children and adolescents with autism, 3 of which confirmed diagnoses, were reviewed. Five types of equine-assisted activities were identified across 25 studies, with reported improvements in behavior, social interaction, and communication. Four types of equine-assisted therapies were identified across 8 studies, with reported improvements in motor control and self-care...
July 21, 2017: Journal of Autism and Developmental Disorders
https://www.readbyqxmd.com/read/28733419/myc2-orchestrates-a-hierarchical-transcriptional-cascade-that-regulates-jasmonate-mediated-plant-immunity-in-tomato
#16
Minmin Du, Jiuhai Zhao, David T W Tzeng, Yuanyuan Liu, Lei Deng, Tianxia Yang, Qingzhe Zhai, Fangming Wu, Zhuo Huang, Ming Zhou, Qiaomei Wang, Qian Chen, Silin Zhong, Chang-Bao Li, Chuanyou Li
The hormone jasmonate (JA), which functions in plant immunity, regulates resistance to pathogen infection and insect attack through triggering genome-wide transcriptional reprogramming in plants. We show that the basic helix-loop-helix transcription factor (TF) MYC2 in tomato (Solanum lycopersicum) acts downstream of the JA receptor to orchestrate JA-mediated activation of both the wounding and pathogen responses. Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with RNA sequencing (RNA-seq) assays, we identified 655 MYC2-targeted JA-responsive genes...
July 21, 2017: Plant Cell
https://www.readbyqxmd.com/read/28733251/rna-binding-protein-raly-promotes-protein-arginine-methyltransferase-1-alternatively-spliced-isoform-v2-relative-expression-and-metastatic-potential-in-breast-cancer-cells
#17
Emma Bondy-Chorney, R Mitchell Baldwin, Andréanne Didillon, Benoît Chabot, Bernard J Jasmin, Jocelyn Côté
Aberrant expression of Protein Arginine Methyltransferases (PRMTs) has been observed in several cancer types, including breast cancer. We previously reported that the PRMT1v2 isoform, which is generated through inclusion of alternative exon 2, is overexpressed in breast cancer cells and promotes their invasiveness. However, the precise mechanism by which expression of this isoform is controlled and how it is dysregulated in breast cancer remains unknown. Using a custom RNA interference-based screen, we identified several RNA binding proteins (RBP) which, when knocked down, altered the relative abundance of the alternatively spliced PRMT1v2 isoform...
July 18, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28732491/job-retention-vocational-rehabilitation-for-employed-people-with-inflammatory-arthritis-work-ia-a-feasibility-randomized-controlled-trial
#18
Alison Hammond, Rachel O'Brien, Sarah Woodbridge, Lucy Bradshaw, Yeliz Prior, Kate Radford, June Culley, Diane Whitham, Ruth Pulikottil-Jacob
BACKGROUND: Inflammatory arthritis leads to work disability, absenteeism and presenteeism (i.e. at-work productivity loss) at high cost to individuals, employers and society. A trial of job retention vocational rehabilitation (VR) in the United States identified this helped people keep working. The effectiveness of this VR in countries with different socioeconomic policies and conditions, and its impact on absenteeism, presenteeism and health, are unknown. This feasibility study tested the acceptability of this VR, modified for the United Kingdom, compared to written advice about managing work problems...
July 21, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28732306/photostability-testing-using-online-reactor-hplc-hyphenation-and-mass-spectrometric-compound-identification-illustrated-by-ketoprofen-as-model-compound
#19
Jaber Assaf, Diego Zulkiewicz Gomes, Bernhard Wuest, Maria Kristina Parr
Investigations on the photochemical stability of pharmaceutical substances are mandatory in drug development and licensing as photo-induced degradation of an active pharmaceutical ingredient (API) may not only lead to decreased API concentrations but also to toxic or reactive products. Thus, the US Food and Drug Administration (FDA) and the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) issued Guidance for Industry Q1B "Photostability Testing of New Drug Substances and Products" for testing of pure but also packed drugs...
July 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28732133/ionization-and-collision-induced-dissociation-of-steroid-bis-glucuronides
#20
Argitxu Esquivel, Xavier Matabosch, Aristotelis Kotronoulas, Georgina Balcells, Jesús Joglar, Rosa Ventura
Studies on steroid metabolism are of utmost importance to improve the detection capabilities of anabolic androgenic steroids (AAS) misuse in sports drug testing. In humans, glucuronoconjugates are the most abundant phase II metabolites of AAS. Bis-glucuronidation is a reaction where two separated functional groups on the same molecule are conjugated with glucuronic acid. These metabolites have not been studied in depth for steroids and could be interesting markers for doping control. The aim of the present work was to study the ionization and collision induced dissociation of steroid bis-glucuronides to be able to develop mass spectrometric analytical strategies for their detection in urine samples after AAS administration...
July 21, 2017: Journal of Mass Spectrometry: JMS
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