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https://www.readbyqxmd.com/read/28230329/integrating-trans-abdominal-ultrasonography-with-fecal-steroid-metabolite-monitoring-to-accurately-diagnose-pregnancy-and-predict-the-timing-of-parturition-in-the-red-panda-ailurus-fulgens-styani
#1
Erin Curry, Lissa J Browning, Paul Reinhart, Terri L Roth
Red pandas (Ailurus fulgens styani) exhibit a variable gestation length and may experience a pseudopregnancy indistinguishable from true pregnancy; therefore, it is not possible to deduce an individual's true pregnancy status and parturition date based on breeding dates or fecal progesterone excretion patterns alone. The goal of this study was to evaluate the use of transabdominal ultrasonography for pregnancy diagnosis in red pandas. Two to three females were monitored over 4 consecutive years, generating a total of seven profiles (four pregnancies, two pseudopregnancies, and one lost pregnancy)...
February 23, 2017: Zoo Biology
https://www.readbyqxmd.com/read/28229988/endometrial-microrna-signature-during-the-window-of-implantation-changed-in-patients-with-repeated-implantation-failure
#2
Cheng Shi, Huan Shen, Li-Juan Fan, Jing Guan, Xin-Bang Zheng, Xi Chen, Rong Liang, Xiao-Wei Zhang, Qing-Hua Cui, Kun-Kun Sun, Zhu-Ran Zhao, Hong-Jing Han
BACKGROUND: At present, a diagnostic tool with high specificity for impaired endometrial receptivity, which may lead to implantation failure, remains to be developed. We aimed to assess the different endometrial microRNA (miRNA) signatures for impaired endometrial receptivity by microarray analysis. METHODS: A total of 12 repeated implantation failure (RIF) patients and 10 infertile patients, who conceived and delivered after one embryo transfer attempt, were recruited as RIF and control groups, respectively...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28228320/embryo-multinucleation-at-the-two-cell-stage-is-an-independent-predictor-of-intracytoplasmic-sperm-injection-outcomes
#3
Laurent Desch, Céline Bruno, Maxime Luu, Julie Barberet, Cécile Choux, Marjorie Lamotte, Emeline Schmutz, Paul Sagot, Patricia Fauque
OBJECTIVE: To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN: Retrospective study. SETTING: Hospital. PATIENT(S): Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28224271/the-perfect-womb-promoting-equality-of-fetal-opportunity
#4
Evie Kendal
This paper aims to address how artificial gestation might affect equality of opportunity for the unborn and any resultant generation of "ectogenetic" babies. It will first explore the current legal obstacles preventing the development of ectogenesis, before looking at the benefits of allowing this technology to control fetal growth and development. This will open up a discussion of the treatment/enhancement divide regarding the use of reproductive technologies, a topic featured in various bioethical debates on the subject...
February 21, 2017: Journal of Bioethical Inquiry
https://www.readbyqxmd.com/read/28223401/transcriptome-analyses-of-rhesus-monkey-pre-implantation-embryos-reveal-a-reduced-capacity-for-dna-double-strand-break-repair-in-primate-oocytes-and-early-embryos
#5
Xinyi Wang, Denghui Liu, Dajian He, Shengbo Suo, Xian Xia, Xiechao He, Jing-Dong Han, Ping Zheng
Pre-implantation embryogenesis encompasses several critical events including genome reprogramming, zygotic genome activation (ZGA), and cell fate commitment, most of which remain mechanistically unclear in primates. In addition, primates display a high rate of embryo wastage without any clear molecular basis. Understanding the factors involved in genome reprogramming and ZGA will help the generation of induced pluripotent stem cells with high efficiency. Moreover, explaining the molecular basis responsible for embryo wastage in primates will greatly expand our knowledge of species evolution...
February 21, 2017: Genome Research
https://www.readbyqxmd.com/read/28222446/the-timing-of-embryo-transfer-catheter-removal-should-it-be-delayed-or-done-immediately-a-prospective-randomized-trial
#6
Belgin Devranoğlu, Özkan Özdamar, Yiğit Çakıroğlu, Mehmet Küçükbaş, Meryem K Eken, Emek Doğer
BACKGROUND/AIMS: Data on the timing of catheter removal technique following embryo transfer (ET) are quite limited. We aimed to compare the reproductive outcomes of intracytoplasmic sperm injection (ICSI)/ET cycles in which the transfer catheter was removed immediately with those in which the catheter was removed after a delay period and hereby to evaluate the impact that the time interval before removal of the catheter following embryo deposit may have on the fertility outcomes. METHODS: A prospective randomized study was designed...
February 22, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28222427/impact-of-fmr1-pre-mutation-status-on-blastocyst-development-in-patients-undergoing-pre-implantation-genetic-diagnosis
#7
Anne P Hutchinson, Nigel Pereira, Debra P Lilienthal, Siobhán Coveney, Jovana P Lekovich, Rony T Elias, Zev Rosenwaks
BACKGROUND/AIMS: The study aimed to investigate the impact of fragile X mental retardation 1 (FMR1) pre-mutation status on blastocyst development in patients undergoing pre-implantation genetic diagnosis (PGD). METHODS: Case-control study of patients <40 years undergoing PGD at blastocyst stage for FMR1 pre-mutation status. Age-matched patients undergoing PGD for other single gene disorders were considered controls. Blastocyst development, calculated per metaphase II (MII) oocyte retrieved and per 2 pronuclear (2PN) embryos, was compared between the 2 groups...
February 22, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28219760/maternal-exposure-to-perfluorooctanoic-acid-inhibits-luteal-function-via-oxidative-stress-and-apoptosis-in-pregnant-mice
#8
Yilu Chen, Ling Zhou, Jingjie Xu, Lu Zhang, Mo Li, Xingxing Xie, Yajuan Xie, Dan Luo, Dalei Zhang, Xiaochun Yu, Bei Yang, Haibin Kuang
Perfluorooctanoic acid (PFOA) is a synthetic perfluorinated compound, which has been reported to exert adverse effect on the pregnancy. However, whether it is associated with alteration of luteal function remains unknown. Mice were administered PFOA by gavage from gestational days (GD) 1 to 7 or 13. PFOA treatment did not significantly affect numbers of embryo implantation. Nevertheless, on GD 13, 10mg/kg PFOA treatment significantly increased numbers of resorbed embryo. Furthermore, PFOA exposure markedly reduced serum progesterone levels but did not affect estradiol levels...
February 17, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28216909/aneuploidy-screening-by-array-comparative-genomic-hybridization-improves-success-rates-of-in-vitro-fertilization-a-multicenter-indian-study
#9
Aditi Kotdawala, Deven Patel, Javier Herrero, Rajni Khajuria, Nalini Mahajan, Manish Banker
OBJECTIVE: To evaluate the usefulness of preimplantation genetic screening (PGS) using array comparative genomic hybridization (aCGH) in the Indian population. MATERIALS AND METHODS: This is a retrospective, multicenter study including 235 PGS cycles following intracytoplasmic sperm injection performed at six different infertility centers from September 2013 to June 2015. Patients were divided as per maternal age in several groups (<35, 35-36, 37-38, 39-40, and >40 years) and as per indication for undergoing PGS...
October 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28215431/global-decrease-in-the-expression-of-signalling-pathways-genes-in-murine-uterus-during-preimplantation-pregnancy
#10
Katarzyna Buska, Anna Ewa Kedzierska, Anna Slawek, Anna Chelmonska-Soyta
Early preimplantation embryo-maternal communication is crucial for the establishment and development of pregnancy. Though the involvement of several candidate genes and proteins in this complex event has been described, the hierarchy of molecular networks governing this communication remains unknown. The primary objective of this study was to determine whether the presence of embryos in the uterine lumen stimulated or inhibited gene expression in the uterine tissue on day 3.5 post coitum. To answer this question, we investigated the gene expression of dedicated signal transduction pathways in the uterus of CD-1 mice during the preimplantation stage of pregnancy and compared this expression to mice with induced pseudopregnancy...
February 15, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28214855/fatty-acid-binding-protein-4-in-endometrial-epithelium-is-involved-in-embryonic-implantation
#11
Peng Wang, Qiuyuan Zhu, Huilian Peng, Mengkai Du, Minyue Dong, Hanzhi Wang
AIMS: To clarify the role of fatty acid-binding protein 4 (FABP4) of endometrial epithelial cell in the establishment and maintenance of pregnancy and the involvement in the pathogenesis of pregnancy loss. METHODS: The expression of FABP4 and uterine receptive factor (LIF, Integrin-β3 and Claudin 4) was determined by Western blotting or quantitative PCR. FABP4 siRNA was used to silence FABP4 while FABP4 inhibitor was used to inhibit the function of FABP4 in endometrial epithelial cell...
January 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28211357/preimplantation-genetic-diagnosis-and-screening-by-array-comparative-genomic-hybridisation-experience-of-more-than-100-cases-in-a-single-centre
#12
J Fc Chow, W Sb Yeung, V Cy Lee, E Yl Lau, P C Ho, E Hy Ng
INTRODUCTION: Preimplantation genetic screening has been proposed to improve the in-vitro fertilisation outcome by screening for aneuploid embryos or blastocysts. This study aimed to report the outcome of 133 cycles of preimplantation genetic diagnosis and screening by array comparative genomic hybridisation. METHODS: This study of case series was conducted in a tertiary assisted reproductive centre in Hong Kong. Patients who underwent preimplantation genetic diagnosis for chromosomal abnormalities or preimplantation genetic screening between 1 April 2012 and 30 June 2015 were included...
February 17, 2017: Hong Kong Medical Journal, Xianggang Yi Xue za Zhi
https://www.readbyqxmd.com/read/28202182/implantation-and-extravillous-trophoblast-invasion-from-rare-archival-specimens-to-modern-biobanking
#13
Gerit Moser, Berthold Huppertz
Extravillous trophoblast invasion serves to attach the placenta to the uterus and to enable access to nutrients for the embryo throughout pregnancy - secretions of the uterine glands in the first trimester, maternal blood in the second and third trimester. For assessing extravillous trophoblast invasion, histology (in combination with immunohistochemistry) still plays a major role in placental research. This is especially true for the re-assessment of rare archival specimens from early human implantation sites or placenta in utero with the background of recent knowledge which may help to strengthen current hypotheses...
February 8, 2017: Placenta
https://www.readbyqxmd.com/read/28202179/effects-of-melatonin-in-rats-in-the-initial-third-stage-of-pregnancy-exposed-to-sub-lethal-doses-of-herbicides
#14
Lécio Leone de Almeida, Álvaro Aguiar Coelho Teixeira, Anísio Francisco Soares, Franklin Magliano da Cunha, Valdemiro Amaro da Silva, Leucio Duarte Vieira Filho, Valéria Wanderley-Teixeira
: Exposure to the herbicides Paraquat (PQ) and Roundup(®) may cause cell lesions due to an increase in oxidative stress levels in different biological systems, even in the reproductive system. OBJECTIVE: Evaluate the possible changes in reproductive parameters and hepatic, as well as its prevention by simultaneous application of melatonin. METHODS: Thirty-five female rats at the age of 3 months were divided into seven groups: three groups exposed to sub-lethal doses of the herbicides PQ (50mg/kg) and Roundup(®) (500mg/kg) (n=5, G2, G3 and G4); three groups exposed to herbicides and simultaneous treatment with 10mg/kg of Melatonin (n=5, G5, G6 and G7) and control group (n=5, G1) from the first to the seventh day of pregnancy...
February 12, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28191608/experiences-of-pre-implantation-genetic-diagnosis-pgd-in-sweden-a-three-year-follow-up-of-men-and-women
#15
Stina Järvholm, Ann Thurin-Kjellberg, Malin Broberg
Men and women with a hereditary genetic disease are faced with different options when they wish to become parents. One is pre-implantation genetic diagnosis (PGD) which is a combination of in vitro fertilization (IVF) and genetic analysis of the embryo before implantation. The present study focused on men and women's psychological experiences of PGD three years after applying for PGD. Nineteen women and seventeen men (i.e. seventeen couples and two women) participated. The interviews were analysed by thematic method...
February 12, 2017: Journal of Genetic Counseling
https://www.readbyqxmd.com/read/28188592/redirecting-reproductive-immunology-research-toward-pregnancy-as-a-period-of-temporary-immune-tolerance
#16
Norbert Gleicher, Vitaly A Kushnir, David H Barad
Referring to two recent publications, we here propose that clinical reproductive immunology has for decades stagnated because reproductive medicine, including assisted reproduction (AR), has failed to accept embryo implantation as an immune system-driven process, dependent on establishment of maternal tolerance toward the implanting fetal semi-allograft (and complete allograft in cases of oocyte donation). Pregnancy represents a biologically unique period of temporary (to the period of gestation restricted) tolerance, otherwise only known in association with parasitic infections...
February 10, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28187504/extreme-spermatogenesis-failure-andrological-phenotype-and-intracytoplasmic-sperm-injection-outcomes
#17
P Plouvier, A-L Barbotin, F Boitrelle, D Dewailly, V Mitchell, J-M Rigot, V Lefebvre-Khalil, G Robin
Patients with very low sperm count through direct sperm examination can exhibit extreme oligozoospermia or cryptozoospermia (after centrifugation). The management of these patients is a real challenge for both clinicians and biologists. In this retrospective and comparative cohort study, we compared the andrological phenotype of patients with extreme alterations of spermatogenesis and assessed whether the origin of spermatozoa (testicular or ejaculate) had any influence on intracytoplasmic sperm injection (ICSI) outcomes...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28185855/biphasic-effect-of-alpha-linolenic-acid-on-glucose-induced-dysmorphogenesis-and-lipoperoxidation-in-whole-rat-embryo-in-culture
#18
Gladys Chirino-Galindo, Jorge-Israel Barrera-Argüelles, Ninna-Leslie Trejo-González, Ricardo Mejía-Zepeda, Martín Palomar-Morales
Type 1 diabetes mellitus complicated with pregnancy, know as diabetic embryopathy, is the cause of neonatal malformations and low for gestational age neonates. With the use of the whole-embryo culture system, it has been demonstrated that high glucose causes embryo dysmorphogenesis, and that oxidative stress appears to be the main mechanism. In recent years, beneficial effect of omega-3 fatty acids has been demonstrated in various diabetic models, and in diabetic complications. Since diabetic embryopathy is mediated probably through membrane lipoperoxidation, This study was designed to find if omega-3 fatty acids could ameliorate the effect of high glucose over the dysmorphogenesis of whole rat embryo in culture...
February 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28185345/the-female-reproduction-and-senescence-nexus
#19
Cielo Mae D Marquez, Joyce A Ibana, Michael C Velarde
Successful pregnancy is regulated by several soluble factors that are differentially expressed throughout gestation. These factors are important to initiate and establish embryo implantation and parturition. Senescent cells, which undergo permanent cell proliferation arrest in response to stress, also produce several secreted factors, referred to as the senescence-associated secretory phenotype (SASP). Here, we review some of the secreted factors found during early and late pregnancy and compare their expression profile with those of the SASP...
February 10, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28183999/gain-of-function-%C3%AE-catenin-in-uterine-mesenchyme-leads-to-impaired-implantation-and-decidualization
#20
Amanda Lynn Patterson, Jamieson Pirochta, Stephanie Tufano, Jose Teixeira
Embryo implantation and endometrial decidualization are critical events that occur during early pregnancy in humans and mice, and perturbation in either can result in infertility. WNT signaling through the canonical β-catenin pathway plays a pivotal role in embryonic Müllerian duct development, postnatal uterine maturation, and establishment of pregnancy. Loss of β-catenin in the Müllerian duct mesenchyme (MDM)-derived stroma and myometrium results in impaired decidualization and infertility; whereas gain of function (GOF) results in formation of mesenchymal tumors and sub-fertility attributed to malformed oviducts...
February 9, 2017: Journal of Endocrinology
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