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https://www.readbyqxmd.com/read/28730230/magnetic-nanoparticles-based-therapy-for-malignant-mesothelioma
#1
Ion Mîndrilă, Sandra Alice Buteică, Dan Eduard Mihaiescu, Florin Burada, Bogdan Mîndrilă, Maria Cristina Predoi, Ionica Pirici, Adrian Fudulu, Octavian Croitoru
This work was aimed to analyze the versatility of the chick embryo chorioallantoic membrane (CAM) as in vivo model for the study of the malignant pleural mesothelioma (MPM) and the therapeutic potential of Fe3O4÷salicylic acid magnetic nanoparticles (SaMNPs) on MPM cells. The antitumor effects of SaMNPs were studied by in vitro and in vivo tests on CARM-L12 TG3 rat malignant mesothelioma cells and human MPM xenografts implanted on CAMs. In order to assess the human MPM xenograft growth characteristics, calretinin, HBME-1 (Hector Battifora mesothelial epitope-1), and cytokeratins immunohistochemical stainings were performed...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28728562/low-oxygen-tension-increases-mitochondrial-membrane-potential-and-enhances-expression-of-antioxidant-genes-and-implantation-protein-of-mouse-blastocyst-cultured-in-vitro
#2
Yun-Yi Ma, Huei-Wen Chen, Chii-Ruey Tzeng
BACKGROUND: In human IVF, embryos cultured with a lower O2 tension (5%) can give rise to higher success rates when compared with normoxic conditions (20%). However, the mechanisms behind the beneficial effects of reduced oxygen tension in embryogenesis remain unclear. The aim of this study was to evaluate the expression of oxygen related and antioxidant genes and mitochondrial function in mouse embryo cultured under hypoxic and normoxic conditions, to investigate the beneficial effect of low oxygen tension in preimplantation embryogenesis...
July 20, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28727117/relationship-between-ovulation-rate-and-embryonic-characteristics-in-gilts-at-35-d-of-pregnancy
#3
C L A Da Silva, M L W J Broekhuijse, B F A Laurenssen, H A Mulder, E F Knol, B Kemp, N M Soede
This study investigated the relationship between ovulation rate (OR) and embryonic characteristics in gilts. Landrace ( = 86) and Yorkshire x Landrace ( = 212) gilts were inseminated with semen stored for 3 to 5 d (SS1, = 59), 6 to7 d (SS2, = 133), or 8 to 10 d (SS3, = 106), and slaughtered at 35 d of pregnancy. Ovulation rate was assessed by dissection of the corpora lutea on both ovaries. Embryos were classified as vital (VE) by visual appearance and individually weighed (VEg) and the SD of the weight calculated (SDVEg)...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28723687/small-intestine-submucosa-is-a-potential-material-for-intrauterine-adhesions-treatment-in-a-rat-model
#4
Desheng Kong, Lin Zhang, Xin Xu, Jingkun Zhang, Yanan Li, Xianghua Huang
OBJECTIVE: To study whether small intestine submucosa (SIS) could be a treatment option for preventing the formation of intrauterine adhesions (IUAs). METHODS: The IUAs model was established by mechanical curettage and infectious injury in a rat model. One uterine horn of the rat model was treated with the transplantation of SIS (SIS group) and the other one was left without any treatment (control group). The endometrium was evaluated with endometrial thickness, number of glands and fibrosis area...
July 20, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28722155/metabolite-availability-as-a-window-to-view-the-early-embryo-microenvironment-in-vivo
#5
REVIEW
Kailun Hu, Yang Yu
A preimplantation embryo exists independent of blood supply, and relies on energy sources from its in vivo environment (e.g. oviduct and uterine fluid) to sustain its development. The embryos can survive in this aqueous environment because it contains amino acids, proteins, lactate, pyruvate, oxygen, glucose, antioxidants, ions, growth factors, hormones, and phospholipids - albeit the concentration of each component varies by species, stage of the estrous cycle, and anatomical location. The dynamic nature of this environment sustains the early development from the one-cell zygote to blastocyst, and is reciprocally influenced by the embryo at each embryonic stage...
July 19, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28712646/live-births-after-embryo-selection-using-morphokinetics-versus-conventional-morphology-a-retrospective-analysis
#6
Simon Fishel, Alison Campbell, Sue Montgomery, Rachel Smith, Lynne Nice, Samantha Duffy, Lucy Jenner, Kathyrn Berrisford, Louise Kellam, Rob Smith, Ivy D'Cruz, Ashley Beccles
The increasing corpus of clinical studies using time-lapse imaging for embryo selection demonstrates considerable variation in study protocols and only limited-sized study cohorts. Outcome measures are based on implantation or clinical pregnancy; some predict blastulation from early cleavage-stage data, and few have evaluated live birth. Erroneously, most studies treat the embryos as independent variables and do not include patient or treatment variables in the statistical analyses. In this study, cohort size was 14,793 patients and 23,762 cycles...
June 23, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28708504/study-of-lysophosphatidic-acid-receptors-lpars-in-buffalo-uterus-demonstrated-upregulation-of-lpar1-and-lpar6-in-early-pregnancy
#7
Abdul Sadam, Subhashree Parida, Amol R Padol, Ankita D Verma, Naseer Ahmad Baba, Wanta M Khuman, Vivek Srivastava, Manjit Panigrahi, Thakur Uttam Singh, Souvendra Nath Sarkar
Lysophosphatidic acid (LPA) is an important factor involved in embryo implantation and pregnancy establishment in humans and domestic livestock. LPA exerts its action through six G-protein-coupled receptors (LPA1-LPA6). We investigated the types of LPA receptors expressed in buffalo uterus and also their differential expression in the nonpregnant, and early-pregnant endometrium. The nonpregnant, and early-pregnant (<42 days) uteri were collected from the local slaughterhouse. RT-PCR experiments detected mRNAs of all the six LPA receptors (LPAR1-LPAR6) in both nonpregnant, and early-pregnant endometrial tissues...
May 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/28707080/reproductive-outcomes-of-anestrous-goats-supplemented-with-spineless-opuntia-megacantha-salm-dyck-protein-enriched-cladodes-and-exposed-to-the-male-effect
#8
Cesar A Meza-Herrera, Omag Cano-Villegas, Arnoldo Flores-Hernandez, Francisco G Veliz-Deras, Guadalupe Calderon-Leyva, Juan M Guillen-Muñoz, Cristina García de la Peña, Cesar A Rosales-Nieto, Ulises Macias-Cruz, Leonel Avendaño-Reyes
The possible influence of the "male effect" upon reproductive outcomes of adult anestrous goats under marginal rangeland conditions and supplemented with protein-enriched Opuntia megacantha Salm-Dyck was evaluated. Reproductive variables included: estrus percentage (EST, %), estrus latency (ESL, hours), ovulation percentage (OP, %), ovulation rate (OR, units), average largest follicle at ovulation (LFO, mm), largest corpus luteum (LCL, mm), embryo number (EBN, units), and embryo implantation percentage (EIP, %)...
July 13, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28706275/novel-function-of-pregnancy-associated-plasma-protein-a-promotes-endometrium-receptivity-by-up-regulating-n-fucosylation
#9
Ming Yu, Jiao Wang, Shuai Liu, Xiaoqi Wang, Qiu Yan
Glycosylation of uterine endometrial cells plays important roles to determine their receptive function to blastocysts. Trophoblast-derived pregnancy-associated plasma protein A (PAPPA) is specifically elevated in pregnant women serum, and is known to promote trophoblast cell proliferation and adhesion. However, the relationship between PAPPA and endometrium receptivity, as well as the regulation of N-fucosylation remains unclear. We found that rhPAPPA and PAPPA in the serum samples from pregnant women or conditioned medium of trophoblast cells promoted endometrium receptivity in vitro...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705424/post-implantation-development-reveals-that-biopsy-procedure-can-segregate-healthy-from-unhealthy-bovine-embryos-and-prevent-miscarriages
#10
Clara Slade Oliveira, Carolina Capobiango Romano Quintão, Celio de Freitas, Agostinho Jorge Dos Reis Camargo, Raquel Varella Serapião, Luiz Sérgio de Almeida Camargo
Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73...
June 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28703337/paternal-age-as-an-independent-factor-does-not-affect-embryo-quality-and-pregnancy-outcomes-of-testicular-sperm-extraction-intracytoplasmic-sperm-injection-in-azoospermia
#11
Y S Park, S H Lee, C K Lim, H W Choi, J H An, C W Park, H S Lee, J S Lee, J T Seo
This study was performed to evaluate the independent influence of paternal age affecting embryo development and pregnancy using testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) in obstructive azoospermia (OA) and nonobstructive azoospermia (NOA). Paternal patients were divided into the following groups: ≤30 years, 31-35 years, 36-40 years, 41-45 years and ≥46 years. There were no differences in the rates of fertilisation or embryo quality according to paternal and maternal age...
July 13, 2017: Andrologia
https://www.readbyqxmd.com/read/28702979/use-of-platelet-rich-plasma-in-human-infertility
#12
G V L Colombo, V Fanton, D Sosa, E Criado Scholz, J Lotti, S E Aragona, T Lotti
Successful embryo implantation requires good quality embryo but also needs a receptive endometrium site. In our clinical practice, we daily verify that an adequate endometrial growth is reached for successful implantation. To understand whether platelet rich plasma (PRP) can improve endometrium thickness and performance, PRP treatment was carried out after at least three of the classic medical protocols currently in use had been unsuccessfully adopted. Eight patients with more than 3 cryo-transfers cancelled because of failure of endometrial growth, defined as endometrium less than 6 mm, with negative hysteroscopic screening for endometrial pathology, and with negative bacteriologic screening, before present and all previous treatment, were selected to undergo PRP treatment...
July 13, 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28701751/secretome-of-in-vitro-cultured-human-embryos-contains-extracellular-vesicles-that-are-uptaken-by-the-maternal-side
#13
Elisa Giacomini, Riccardo Vago, Ana Maria Sanchez, Paola Podini, Natasa Zarovni, Valentina Murdica, Roberta Rizzo, Daria Bortolotti, Massimo Candiani, Paola Viganò
Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX. The embryonic origin of these EVs has been confirmed by the presence of stemness gene transcripts and their enrichment in the non-classical HLA-G protein...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28697910/duphaston-and-human-menopausal-gonadotropin-protocol-in-normally-ovulatory-women-undergoing-controlled-ovarian-hyperstimulation-during-in%C3%A2-vitro-fertilization-intracytoplasmic-sperm-injection-treatments-in-combination-with-embryo-cryopreservation
#14
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To evaluate endocrine characteristics and clinical outcomes in normal ovulatory patients undergoing controlled ovarian hyperstimulation (COH) with the use of a Duphaston and hMG protocol during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatments in combination with frozen-thawed embryo transfer (FET) compared with the characteristics and outcomes of patients undergoing an Utrogestan and hMG protocol. DESIGN: Prospective controlled study...
July 8, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28695620/correlated-response-in-litter-size-components-in-rabbits-selected-for-litter-size-variability
#15
M J Argente, E W Calle, M L García, A Blasco
A divergent selection experiment for the environmental variability of litter size (Ve) over seven generations was carried out in rabbits at the University Miguel Hernández of Elche. The Ve was estimated as the phenotypic variance within the female, after correcting for year-season and parity-lactation status. The aim of this study was to analyse the correlated responses to selection in litter size components. The ovulation rate (OR) and number of implanted embryos (IE) in females were measured by laparoscopy at 12 day of the second gestation...
July 11, 2017: Journal of Animal Breeding and Genetics, Zeitschrift Für Tierzüchtung und Züchtungsbiologie
https://www.readbyqxmd.com/read/28695395/a-meta-analysis-of-atosiban-supplementation-among-patients-undergoing-assisted-reproduction
#16
Jie Li, Yang Chen, Anran Wang, Haiying Zhang
OBJECTIVES: Evidence for the efficacy of atosiban in improving pregnancy outcomes among patients undergoing assisted reproductive technology (ART) remains inconsistent. METHODS: The PubMed, EMBASE, and CNKI databases were searched using keywords such as 'atosiban', 'infertility', and 'ART'. Studies that explored the efficacy of atosiban in the field of ART for patients with embryo transfer (ET) were included if they evaluated pregnancy outcomes. Combing using subgroups and sensitivity analysis were conducted, and risk ratios (RRs), and 95% confidence intervals (CIs) were calculated...
July 10, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28693372/prostaglandin-e-pathway-in-uterine-tissue-during-window-of-preimplantation-in-female-mice-mated-with-intact-and-seminal-vesicle-excised-male
#17
Mahnaz Shahnazi, Mohammad Nouri, Gisou Mohaddes, Zeinab Latifi, Amir Fattahi, Mustafa Mohammadi
Prostaglandin E2 (PGE2) has been introduced as an important factor for embryo implantation. So the present study was carried out to evaluate the effect of seminal fluid (SF) on PGE2 pathway in uterus tissues of mice during window of preimplantation. The messenger RNA (mRNA) expressions of microsomal PGE synthase (mPGES) and cytosolic PGE synthase (cPGES) as well as protein expression of PGE receptor 2 and 4 (EP2 and EP4) were determined in uterine tissue of control and seminal vesicle-excised (SVX)-mated female mice during days 1 to 5 of pregnancy using real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28689770/vip-induces-the-decidualization-program-and-conditions-the-immunoregulation-of-the-implantation-process
#18
Esteban Grasso, Soledad Gori, Daniel Paparini, Elizabeth Soczewski, Laura Fernández, Lucila Gallino, Gabriela Salamone, Gustavo Martinez, Marcela Irigoyen, Claudio Ruhlmann, Claudia Pérez Leirós, Rosanna Ramhorst
The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation...
July 6, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28688214/uterine-molecular-changes-for-non-invasive-embryonic-attachment-in-the-marsupials-macropus-eugenii-macropodidae-and-trichosurus-vulpecula-phalangeridae
#19
Melanie K Laird, Jessica R Dargan, Lillian Paterson, Christopher R Murphy, Bronwyn M McAllan, Geoff Shaw, Marilyn B Renfree, Michael B Thompson
Pregnancy in mammals requires remodelling of the uterus to become receptive to the implanting embryo. Remarkably similar morphological changes to the uterine epithelium occur in both eutherian and marsupial mammals, irrespective of placental type. Nevertheless, molecular differences in uterine remodelling indicate that the marsupial uterus employs maternal defences, including molecular reinforcement of the uterine epithelium, to regulate embryonic invasion. Non-invasive (epitheliochorial) embryonic attachment in marsupials likely evolved secondarily from invasive attachment, so uterine defences in these species may prevent embryonic invasion...
July 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28687174/pre-implantation-hla-matching-the-production-of-a-saviour-child
#20
REVIEW
Georgia Kakourou, Christina Vrettou, Maria Moutafi, Joanne Traeger-Synodinos
Pre-implantation genetic diagnosis (PGD) requires the use of assisted reproductive technology (ART) to create several pre-implantation-stage embryos, followed by biopsy of embryonic cells for genetic testing and transfer of selected embryos to the womb to establish a pregnancy. HLA typing of ART-created embryos was first reported in 2001. The aim is to establish a pregnancy that is HLA-compatible with an affected sibling who requires haematopoietic stem cell transplantation. HLA-typing can be performed with or without PGD for the exclusion of a single-gene disorder...
June 16, 2017: Best Practice & Research. Clinical Obstetrics & Gynaecology
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