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Myocardial viability

Eakkapote Prompunt, Jantira Sanit, Stephanie Barrère-Lemaire, Joel Nargeot, Hannah Noordali, Melanie Madhani, Sarawut Kumphune
Protease enzymes generated from injured cells and leukocytes are the primary cause of myocardial cell damage following ischemia/reperfusion (I/R). The inhibition of protease enzyme activity via the administration of particular drugs may reduce injury and potentially save patients' lives. The aim of the current study was to investigate the cardioprotective effects of treatment with recombinant human secretory leukocyte protease inhibitor (rhSLPI) on in vitro and ex vivo models of myocardial I/R injury. rhSLPI was applied to isolated adult rat ventricular myocytes (ARVMs) subjected to simulated I/R and to ex vivo murine hearts prior to I/R injury...
June 2018: Experimental and Therapeutic Medicine
Chunfu Wan, Yueyue Wei, Jianguo Ma, Xiaoyong Geng
The present study aimed to investigate the protective effects and molecular mechanisms of scoparone on ischemia‑reperfusion (I/R) injury in primary cultured cardiac myocytes and rats. An in vivo rat model of I/R injury and an in vitro primary cultured cardiac myocyte model of oxygen‑glucose deprivation/reoxygenation were used to investigate the protective effects of scoparone. Cell viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD), creatine kinase (CK) and malondialdehyde (MDA) levels, and reactive oxygen species (ROS) production were subsequently measured...
June 4, 2018: Molecular Medicine Reports
Zhengwen Zhou, Yue Zhang, Luning Lin, Jianmei Zhou
Apigenin, a flavonoid with multiple physiological and pharmacological activities, is associated with the prevention of cardiovascular diseases. The present study aimed to examine the roles and mechanisms of apigenin in the apoptosis of H9C2 rat cardiomyocytes, which were subjected to myocardial ischemia‑reperfusion (MI/R) injury. Cell viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cellular apoptosis were evaluated using cell counting kit‑8 assays and flow cytometry...
May 31, 2018: Molecular Medicine Reports
Masaaki Takeuchi, Victor Chien-Chia Wu
PURPOSE OF REVIEW: Myocardial strain imaging has gained popularity during the last decade in various clinical scenarios. The objective of this article was to review the potential application of two-dimensional (2D) and three-dimensional (3D) strains in patients with coronary artery disease. RECENT FINDINGS: Reports on the diagnostic accuracy of myocardial deformation analysis using 2D and 3D speckle-tracking analyses to detect significant coronary stenosis at rest or during stress and to evaluate myocardial viability are limited...
June 7, 2018: Current Opinion in Cardiology
Yang Zhu, Yasumoto Matsumura, Murugesan Velayutham, Lesley M Foley, T Kevin Hitchens, William R Wagner
Tissue damage and the impairment of regenerative processes by excessive reactive oxygen species (ROS) contributes to the pathogenesis of various diseases in soft tissues including diabetes, atherosclerosis, Parkinson's disease and myocardial ischemic/reperfusion injury. In this study, a thermally responsive injectable hydrogel poly(NIPAAm-co-VP-co-MAPLA-co-MATEMPO) (pNVMT, NIPAAm: N-isopropylacrylamide, VP: vinylpyrrolidone, MAPLA: methacrylate-polylactide, MATEMPO: methacrylate-TEMPO, TEMPO: 4-amino-TEMPO or 4-Amino-2,2,6,6-tetramethylpiperidine-1-oxyl) incorporating recyclable ROS scavenging nitroxide radicals on the polymer backbone was developed to locally control adverse tissue effects from free radical generation...
May 31, 2018: Biomaterials
Xiaozhe Yang, Lin Feng, Yannan Zhang, Hejing Hu, Yanfeng Shi, Shuang Liang, Tong Zhao, Yang Fu, Junchao Duan, Zhiwei Sun
Although the strongly causal associations were between fine particulate matter (PM2.5 ) and cardiovascular disease, the toxic effect and potential mechanism of PM2.5 on heart was poorly understood. Thus, the aim of this study was to evaluate the cardiac toxicity of PM2.5 exposure on human cardiomyocytes (AC16). The cell viability was decreased while the LDH release was increased in a dose-dependent way after AC16 exposed to PM2.5 . The reactive oxygen species (ROS) generation and production of malondialdehyde (MDA) were increased followed by the decreasing in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)...
June 6, 2018: Ecotoxicology and Environmental Safety
Liang Qin, Shuxia Fan, Rongbo Jia, Yongxuan Liu
AIM: Myocardial ischemia (MI) is a leading cause of morbidity and mortality which makes the prevention and control of MI tremendously important. We aimed to explore the functional roles of ginsenoside (Gin) Rg1 in cardiomyocytes under hypoxia and to clarify underlying mechanisms. MAIN METHODS: Hypoxia-induced H9c2 cell injury was evaluated by alterations of cell viability, apoptosis and autophagy. Then, effects of Gin Rg1 on hypoxia-induced cell injury were measured...
June 1, 2018: Die Pharmazie
Katsuhisa Sakaguchi, Yuto Hinata, Yuki Kagawa, Kiyotaka Iwasaki, Satoshi Tsuneda, Tatsuya Shimizu, Mitsuo Umezu
Assembling three-dimensional (3D) tissues from single cells necessitates the use of various advanced technological methods because higher-density tissues require numerous complex capillary structures to supply sufficient oxygen and nutrients. Accordingly, creating healthy culture conditions to support 3D cardiac tissues requires an appropriate balance between the supplied nutrients and cell metabolism. The objective of this study was to develop a simple and efficient method for low-temperature cultivation (< 37 °C) that decreases cell metabolism for facilitating the buildup of 3D cardiac tissues...
July 2018: Biochemistry and Biophysics Reports
Wei Li, Jin-Zhuo Ning, Fang Cheng, Wei-Min Yu, Ting Rao, Yuan Ruan, Run Yuan, Xiao-Bin Zhang, Yang Du, Cheng-Cheng Xiao
BACKGROUND/AIMS: Accumulating evidences has indicated that aberrant expression of long non-coding RNAs (lncRNAs) is tightly associated with the progression of ischemia-reperfusion injury (IRI). Previous studies have reported that lncRNA MALAT1 regulates cell apoptosis and proliferation in myocardial and cerebral IRI. However, the underlying mechanism of MALAT1 in testicular IRI has not been elucidated. METHODS: The levels of MALAT1, some related proteins and apoptosis in the testicular tissues were determined by quantitative real-time PCR, HE staining, immunohistochemistry, western blot and TUNEL assays...
2018: Cellular Physiology and Biochemistry
Nianxin Jiang, Jingwen Xia, Bing Jiang, Yinghui Xu, Yansong Li
TUG1 has been reported to play an important role in various cancer types. However, the study about the function of lncRNA TUG1 in myocardial infarction is limited. This study was aimed to investigate the role of TUG1 in H9c2 cell injury induced by hypoxia and explore its possible molecular mechanism. The proliferation assay, migration assay, invasion assay, and apoptosis assay were performed. RT-PCR was used to determine the relative RNA expression of TUG1, miR-124 and Hic-5. Western blotting was used to detect the expression levels of apoptotic proteins, Hic-5, Sp1, and Survivin...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Bing Xing, Qiao-Ju Li, Hu Li, Sha-Sha Chen, Zhi-Yuan Cui, Jie Ma, Zai-Wei Zhang
Myocardial infarction (MI) is an important cause of cardiovascular disease. microRNAs (miRNAs) have been indicated as pivotal regulators in the physiological and pathological processes of heart diseases. The purpose of this study was to investigate the role of miR-140-5p in hypoxia-induced cell injury in H9c2 cells and its underlying mechanism. H9c2 cells were subjected to hypoxia, before which the expression levels of miR-140-5p and MLK3 were overexpressed or knocked down through transient transfection. The efficiency of transfection was verified by qRT-PCR and Western blotting...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
L Geng, X-T Wang, J Yu, Y-L Yang
OBJECTIVE: To investigate the role of cortistatin (CST) on cyclosporine A (CsA)-induced myocardial apoptosis in rats and determine its effect on the expressions of myocardial apoptosis genes. MATERIALS AND METHODS: H9C2 cells were treated with different concentrations of CsA solution (0.04, 0.2, 1 and 5 μM) for 24, 48 and 72 h, respectively. The cell viability was detected via methyl thiazolyl tetrazolium (MTT) assay, and the appropriate dose and time were compared and determined...
May 2018: European Review for Medical and Pharmacological Sciences
Calvin Yeang, Devin Hasanally, Xuchu Que, Ming-Yow Hung, Aleksandra Stemankovic, David Chan, Rakesh Chaudhary, Victoria Margulets, Andrea L Edel, Masahiko Hoshijima, Yusu Gu, William Bradford, Nancy Dalton, Phuong Miu, David Y C Cheung, Davinder S Jassal, Grant N Pierce, Kirk L Peterson, Lorrie A Kirshenbaum, Joseph L Witztum, Sotirios Tsimikas, Amir Ravandi
Aims: Myocardial ischemia followed by reperfusion (IR) causes an oxidative burst resulting in cellular dysfunction. Little is known about the impact of oxidative stress on cardiomyocyte lipids and their role in cardiac cell death. Our goal was to identify oxidized phosphatidylcholine-containing phospholipids (OxPL) generated during IR, and to determine their impact on cell viability and myocardial infarct size. Methods and Results: OxPL were quantitated in isolated rat cardiomyocytes using mass spectrophotometry following 24 hours of IR...
May 30, 2018: Cardiovascular Research
Yuan Zhang, Wating Su, Qiongxia Zhang, Jinjin Xu, Huimin Liu, Jun Luo, Liying Zhan, Zhongyuan Xia, Shaoqing Lei
Background: Patients with diabetes are more vulnerable to myocardial ischemia reperfusion injury (IRI), which is involved in PKC β 2 activation and mitochondrial dysfunction. Glycine has been documented as a cytoprotective agent to attenuate diabetes-related abnormalities and reduce myocardial IRI, but the underlying mechanisms are still unclear. We determined whether glycine could attenuate high glucose- (HG-) and hypoxia/reoxygenation- (H/R-) induced injury by inhibiting PKC β 2 activation and improving mitochondrial quality in cultured H9C2 cells...
2018: Journal of Diabetes Research
Chin Siang Ong, Lucy Nam, Kingsfield Ong, Aravind Krishnan, Chen Yu Huang, Takuma Fukunishi, Narutoshi Hibino
3D and 4D bioprinting of the heart are exciting notions in the modern era. However, myocardial bioprinting has proven to be challenging. This review outlines the methods, materials, cell types, issues, challenges, and future prospects in myocardial bioprinting. Advances in 3D bioprinting technology have significantly improved the manufacturing process. While scaffolds have traditionally been utilized, 3D bioprinters, which do not require scaffolds, are increasingly being employed. Improved understanding of the cardiac cellular composition and multiple strategies to tackle the issues of vascularization and viability had led to progress in this field...
2018: BioMed Research International
Yihua Liu, Yefan Jiang, Xiaoxi Yang, Bingchuan Geng, Yi Liu, Xiaoke Shang, Jinping Liu, Xiaoli Lan, Nianguo Dong
Background: Myocardial viability assessment is typically performed in patients with coronary artery disease (CAD) and severe left ventricular (LV) dysfunction to identify those who might benefit from revascularization and assist in decision making process. However, the prognostic value of myocardial viability testing remains a debating issue. Methods: Positron Emission Tomography using 18 F-fluorodeoxyglucose (18 FDG-PET) was performed in 81 patients with ischemic LV dysfunction [ejection fraction (EF) ≤35%] for myocardial viability assessment prior to coronary artery bypass surgery...
April 2018: Journal of Thoracic Disease
Ling Wang, Rong Lin, Langtao Guo, Meiman Hong
The present study aimed to investigate whether pretreatment with rosuvastatin (RS) can provide cardioprotection in a myocardial ischemia/reperfusion (MI/R) model. The protective effect of RS on myocardial oxygen‑glucose deprivation/reperfusion (OGD/R) injury was also evaluated by upregulating peroxisome proliferator‑activated receptor‑γ (PPAR‑γ). In the present study, MI/R model was established and activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), creatine kinase‑muscle/brain (CK‑MB), malondialdehyde (MDA), and troponin I/T were measured...
May 23, 2018: Molecular Medicine Reports
Shuang Wang, Yanfang Zhu, Ruixia Qiu
Myocardial ischemic/reperfusion (I/R) injury often leads to irreversible myocardial cell death and even heart failure, with limited therapeutic possibilities. In the present study, we evaluated the protective effects of shikonin (SHK) against hypoxia/reoxygenation (H/R)-induced cardiomyocyte damage and explored the underlying mechanisms. H9C2 cardiomyocytes were pretreated with different doses of SHK prior to H/R exposure. We observed that SHK pretreatment significantly increased cell viability, attenuated LDH release, and suppressed cardiomyocyte apoptosis induced by H/R exposure...
May 25, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Bapi Gorain, Hira Choudhury, Manisha Pandey, Prashant Kesharwani, Muhammad Mustafa Abeer, Rakesh Kumar Tekade, Zahid Hussain
Myocardial infarction (cardiac tissue death) is among the most prevalent causes of death among the cardiac patients due to the inability of self-repair in cardiac tissues. Myocardial tissue engineering is regarded as one of the most realistic strategies for repairing damaged cardiac tissue. However, hindrance in transduction of electric signals across the cardiomyocytes due to insulating properties of polymeric materials worsens the clinical viability of myocardial tissue engineering. Aligned and conductive scaffolds based on Carbon nanotubes (CNT) have gained remarkable recognition due to their exceptional attributes which provide synthetic but viable microenvironment for regeneration of engineered cardiomyocytes...
May 22, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Dongpu Dai, Jian Yang, Chenze Zhao, Huandong Wu, Jie Ding, Xiaotong Sun, Shenjiang Hu
Recent studies have revealed that geranylgeranyl pyrophosphate synthase (GGPPS), a key enzyme involved in protein prenylation, plays a critical role in postnatal heart growth by regulating cardiomyocyte size. However, the role of GGPPS in myocardial ischemia/reperfusion (MIR) injury is still not clear. The objective of this work was to investigate the effect of GGPPS on MIR injury in H9c2 cells subjected to hypoxia/reoxygenation (HR) to mimic MIR. Prior to HR, the cells were transfected with GGPPS, shGGPPS, or shGFP...
May 23, 2018: International Heart Journal
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