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https://www.readbyqxmd.com/read/28550807/effects-of-waterborne-copper-delivered-under-two-different-exposure-and-salinity-regimes-on-osmotic-and-ionic-regulation-in-the-mudflat-fiddler-crab-minuca-rapax-ocypodidae-brachyura
#1
Mariana V Capparelli, John C McNamara, Martin Grosell
The effects of exposure to copper (Cu) on tissue Cu accumulation, on hemolymph osmotic, Na(+) and Cl(-) regulation, and on gill Na(+)/K(+)-ATPase (NKA) and carbonic anhydrase (CA) activities were evaluated in the fiddler crab Minuca rapax. Waterborne copper was delivered to the crabs at one of three salinities (seawater at 25‰ salinity [S] = isosmotic control; distilled water [<0.1‰ S] = hypo-osmotic medium; or 60‰ S = hyper-osmotic seawater) either for 5 days in a 0.5-cm water film containing 0, 50, 150, 250 or 500µg Cu/L with free access to a dry surface, or in crabs fully submerged for 5h at 0, 250 or 500µg Cu/L...
May 24, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28550766/gender-differences-in-quality-of-life-and-functional-disability-for-depression-outpatients-with-or-without-residual-symptoms-after-acute-phase-treatment-in-china
#2
Na Zhao, Xiaohong Wang, Wenyuan Wu, Yongdong Hu, Yajuan Niu, Xueyi Wang, Chengge Gao, Ning Zhang, Yiru Fang, Jizhong Huang, Tiebang Liu, Fujun Jia, Xuequan Zhu, Jian Hu, Gang Wang
BACKGROUND: Depression is associated with substantial personal suffering and reduced quality of life and functioning. The aim of this study was to investigate gender differences on quality of life and functional impairment of outpatients with depression after acute phase treatment. METHODS: 1503 depression outpatients were recruited from eleven hospitals in China. Subjects were evaluated with sociodemographic characteristics, history and self-report instruments, related to severity of symptoms, function and quality of life...
May 17, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28550649/improved-formula-see-text-determination-in-23-na-35-cl-and-17-o-mri-using-iterative-partial-volume-correction-based-on-1-h-mri-segmentation
#3
Sebastian C Niesporek, Reiner Umathum, Thomas M Fiedler, Peter Bachert, Mark E Ladd, Armin M Nagel
OBJECTIVE: Functional parameters can be measured with the help of quantitative non-proton MRI where exact relaxometry parameters are needed. Investigation of [Formula: see text] is often biased by strong partial volume (PV) effects. Hence, in this work a PV correction algorithm approach was evaluated that uses iteratively adapted [Formula: see text]-values and high-resolution structural (1)H data to determine transverse relaxation in non-proton MRI more accurately. MATERIALS AND METHODS: Simulations, a phantom study and in vivo (23)Na, (17)O and (35)Cl MRI measurements of five healthy volunteers were performed to evaluate the algorithm...
May 26, 2017: Magma
https://www.readbyqxmd.com/read/28550644/high-genetic-diversity-of-giardia-duodenalis-assemblage-e-in-pre-weaned-dairy-calves-in-shanghai-china-revealed-by-multilocus-genotyping
#4
Xiaolan Wang, Min Cai, Wen Jiang, Yuping Wang, Yue Jin, Na Li, Yaqiong Guo, Yaoyu Feng, Lihua Xiao
Giardia duodenalis is a common parasitic protozoan in human and animals. Epidemiological and molecular data are available from dairy cattle in many industrialized countries, but information on genetic diversity at multiple genetic loci is limited, especially in pre-weaned dairy calves. In this study, 818 fecal specimens were collected from five dairy cattle farms located in suburbs of Shanghai, China, with two to five samplings per farm. G. duodenalis assemblages and subtypes were determined using multilocus genotyping (MLG) at the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) loci...
May 26, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28550472/salt-induced-na-k-atpase-%C3%AE-%C3%AE-expression-involves-soluble-adenylyl-cyclase-in-endothelial-cells
#5
Mirja Mewes, Johanna Nedele, Katrin Schelleckes, Olga Bondareva, Malte Lenders, Kristina Kusche-Vihrog, Hans-Joachim Schnittler, Stefan-Martin Brand, Boris Schmitz, Eva Brand
High dietary salt intake may lead to vascular stiffness, which predicts cardiovascular diseases such as heart failure, and myocardial and cerebral infarctions as well as renal impairment. The vascular endothelium is a primary target for deleterious salt effects leading to dysfunction and endothelial stiffness. We hypothesize that the Ca(2+)- and bicarbonate-activated soluble adenylyl cyclase (sAC) contributes to Na(+)/K(+)-ATPase expression regulation in vascular endothelial cells and is an important regulator of endothelial stiffness...
May 26, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28550468/probing-the-contractile-vacuole-as-achilles-heel-of-the-biotrophic-grapevine-pathogen-plasmopara-viticola
#6
Viktoria Tröster, Tabea Setzer, Thomas Hirth, Anna Pecina, Andreas Kortekamp, Peter Nick
The causative agent of Grapevine Downy Mildew, the oomycete Plasmopara viticola, poses a serious threat to viticulture. In the current work, the contractile vacuole of the zoospore is analysed as potential target for novel plant protection strategies. Using a combination of electron microscopy, spinning disc confocal microscopy, and video differential interference contrast microscopy, we have followed the genesis and dynamics of this vacuole required during the search for the stomata, when the non-walled zoospore is exposed to hypotonic conditions...
May 26, 2017: Protoplasma
https://www.readbyqxmd.com/read/28550312/dependence-of-solidification-for-bi2te3-xsex-alloys-on-their-liquid-states
#7
Yuan Yu, Zhan Wu, Oana Cojocaru-Mirédin, Bin Zhu, Xiao-Yu Wang, Na Gao, Zhong-Yue Huang, Fang-Qiu Zu
The resistivity versus temperature (ρ-T) behaviours of liquid n-type Bi2Te3-x Se x (x = 0.3, 0.45 and 0.6) alloys are explored up to 1050 °C. A clear hump is observed on all ρ-T curves of the three studied Bi2Te3-x Se x melts during the heating process, which suggests that a temperature-induced liquid-liquid structural transition takes place in the melts. Based on this information, the solidification behaviours and microstructures of the alloys with different liquid states are investigated. The samples that experienced liquid structural transition show that the nucleation and growth undercooling degrees are conspicuously enlarged and the solidification time is shortened...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28550304/electronic-supplementary-materialidentification-of-a-na-k-atpase-inhibition-independent-proarrhythmic-ionic-mechanisms-of-cardiac-glycosides
#8
Cai Hong Koh, Jianjun Wu, Ying Ying Chung, Zhenfeng Liu, Rong-Rong Zhang, Ketpin Chong, Vladimir Korzh, Sherwin Ting, Steve Oh, Winston Shim, Hai-Yan Tian, Heming Wei
The current study explored the Na(+)/K(+)-ATPase (NKA) inhibition-independent proarrhythmic mechanisms of cardiac glycosides (CGs) which are well-known NKA inhibitors. With the cytosolic Ca(2+) chelated by EGTA and BAPTA or extracellular Ca(2+) replaced by Ba(2+), effects of bufadienolides (bufalin (BF) and cinobufagin (CBG)) and cardenolides (ouabain (Oua) and pecilocerin A (PEA)) on the L-type calcium current (I Ca,L) were recorded in heterologous expression Cav1.2-CHO cells and human embryonic stem cell-derived cardiomyocytes (hESC-CMs)...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28550085/knockdown-of-sodium-calcium-exchanger-1-induces-epithelial-to-mesenchymal-transition-in-kidney-epithelial-cells
#9
Sona Lakshme Balasubramaniam, Anilkumar Gopalakrishnapillai, Nicholas J Petrelli, Sonali P Barwe
Mesenchymal to epithelial transition (MET) and epithelial to mesenchymal transition (EMT) are important processes in kidney development. Failure to undergo MET during development leads to the initiation of Wilms tumor (WT) whereas EMT contributes to the development of renal cell carcinomas (RCC). The role of calcium regulators in governing these processes is becoming evident. We demonstrated earlier that Na+/Ca2+ exchanger 1 (NCX1), a major calcium exporter in renal epithelial cells, regulates epithelial cell motility...
May 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28549711/symptomatic-lumbar-facet-synovial-cysts-clinical-outcomes-following-percutaneous-ct-guided-cyst-rupture-with-intraarticular-steroid-injection
#10
Steffen J Haider, Nu R Na, Clifford J Eskey, Jessica G Fried, Natalie Y Ring, Mike H Bao, David A Pastel
PURPOSE: To evaluate clinical outcomes following percutaneous rupture of symptomatic lumbar facet synovial cysts (LFSCs) with intraarticular steroid injection. MATERIALS AND METHODS: In this retrospective review, 44 consecutive patients with symptomatic LFSCs received primary treatment with CT-guided synovial cyst rupture with intraarticular steroid injection. Outcomes questionnaires were obtained before and 1, 4, 26, and 52 weeks after LFSC rupture. Assessment included pain medication use and numeric rating scale (NRS), Oswestry Disability Index (ODI), and 12-item short form health survey (SF-12) physical and mental composite scores (PCS and MCS)...
May 24, 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/28549486/the-effect-of-chlormadinone-acetate-on-odontogenic-differentiation-of-human-dental-pulp-cells-in-vitro-study
#11
Se-Min Kim, Bin-Na Lee, Jeong-Tae Koh, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Kyung-San Min, Yun-Chan Hwang
BACKGROUND: Chlormadinone acetate (CMA) is a derivative of progesterone and is used as an oral contraceptive. The aim of this study was to investigate the effects of CMA on odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) and related signaling pathways. METHODS: Cell viability was determined by the water-soluble tetrazolium (WST)-1 assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction using odontogenic marker genes, such as alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1)...
May 26, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28549291/antidiabetogenic-efficiency-of-menthol-improves-glucose-homeostasis-and-attenuates-pancreatic-%C3%AE-cell-apoptosis-in-streptozotocin-nicotinamide-induced-experimental-rats-through-ameliorating-glucose-metabolic-enzymes
#12
Udaiyar Muruganathan, Subramani Srinivasan, Veerasamy Vinothkumar
The phytochemical, menthol, has been reported to play many beneficial roles. However, under diabetic conditions, there is no detail mechanism of its beneficial action in the glucose homeostasis. The present study, we investigated to explore the role of menthol, on the glucose metabolic enzymes and pancreatic islet cell apoptosis of streptozotocin-nicotinamide (STZ-NA) induced diabetes in rats. Diabetes was induced by single intraperitoneal (i.p.) injection of STZ (50mg/kg/b.w.) and NA (110mg/kg/b.w.). Diabetic rats were treated with different dose of menthol (25, 50, and 100mg/kg/b...
May 23, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28549236/assessment-of-pathogenicity-and-antigenicity-of-american-lineage-influenza-h5n2-viruses-in-taiwan
#13
Chun-Yang Lin, Min-Yuan Chia, Po-Ling Chen, Chia-Tsui Yeh, Ming-Chu Cheng, Ih-Jen Su, Min-Shi Lee
During December 2003 and March 2004, large scale epidemics of low-pathogenic avian influenza (LPAI) H5N2 occurred in poultry farms in central and southern Taiwan. Based on genomic analysis, these H5N2 viruses contain HA and NA genes of American-lineage H5N2 viruses and six internal genes from avian influenza A/H6N1 viruses endemic in poultry in Taiwan. After disappearing for several years, these novel influenza H5N2 viruses caused outbreaks in poultry farms again in 2008, 2010 and 2012, and have evolved into high pathogenic AI (HPAI) since 2010...
May 23, 2017: Virology
https://www.readbyqxmd.com/read/28548853/a-series-of-fluorescent-and-colorimetric-chemodosimeters-for-selective-recognition-of-cyanide-based-on-fret-mechanism
#14
Ying-Xi Hua, Yongliang Shao, Ya-Wen Wang, Yu Peng
A series of fluorescence "turn-on" probes (PY, AN, NA, B1 and B2) have been developed and successfully applied to detect cyanide anions based on Michael-addition reaction and FRET mechanism. These probes demonstrated good selectivity, high sensitivity and very fast recognition for CN-. Especially, the fluorescence response of probe NA finished within 3 seconds. Low LODs (down to 63 nM) are also obtained in these probes with remarkable fluorescence enhancement factors. In addition, fluorescence colors of these probes turned to blue, yellow or orange upon sensing CN-...
May 26, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28548675/inducible-bcl-2-gene-rna-interference-mediated-by-aptamer-integrated-hdv-ribozyme-switch
#15
Yuanyuan Zhang, Jine Wang, Hui Cheng, Na Sun, Min Liu, Zhengyan Wu, Renjun Pei
The regulation of RNA interference (RNAi) could be a powerful method for the study of temporal and dose dependent effects of gene expression. In this study, we designed the hepatitis delta virus (HDV) ribozyme with an embedded theophylline aptamer as the sensor domain and the pri-miRNA of endogenous gene Bcl-2 as the effector domain to engineer an RNAi-regulatory device in MCF-7 cells. The system allowed us to control gene expression by adding theophylline into the culture media in a dose dependent fashion...
May 26, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28548486/elucidation-of-the-oxygen-surface-kinetics-in-a-coated-dual-phase-membrane-for-enhancing-oxygen-permeation-flux
#16
Beom Tak Na, Jeong Hwan Park, Jong Hyuk Park, Ji Haeng Yu, Jong Hoon Joo
The dual-phase membrane has received much attention as the solution to the instability of the oxygen permeation membrane. It has been reported that the oxygen flux of the dual-phase membrane is greatly enhanced by the active coating layer. However, there has been little discussion about the enhancement mechanism by surface coating in the dual-phase membrane. This study investigates the oxygen flux of Ce0.9Gd0.1O2-δ-La0.7Sr0.3MnO3±δ (GDC 80 vol.%/LSM 20 vol.%) composite membrane depending on the oxygen partial pressure (P_(O_2 )) to elucidate the mechanism of enhanced oxygen flux by the surface modification in the fluorite-rich phase dual-phase membrane...
May 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28548182/structural-and-electronic-properties-of-defects-at-grain-boundaries-in-cuinse2
#17
R Saniz, J Bekaert, B Partoens, D Lamoen
We report on a first-principles study of the structural and electronic properties of a Σ3 (112) grain boundary model in CuInSe2. The study focuses on a coherent, stoichiometry preserving, cation-Se terminated grain boundary, addressing the properties of the grain boundary as such, as well as the effect of well known defects in CuInSe2. We show that in spite of its apparent simplicity, such a grain boundary exhibits a very rich phenomenology, providing an explanation for several of the experimentally observed properties of grain boundaries in CuInSe2 thin films...
May 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28547895/review-of-flexible-temperature-sensing-networks-for-wearable-physiological-monitoring
#18
REVIEW
Qiao Li, Li-Na Zhang, Xiao-Ming Tao, Xin Ding
Physiological temperature varies temporally and spatially. Accurate and real-time detection of localized temperature changes in biological tissues regardless of large deformation is crucial to understand thermal principle of homeostasis, to assess sophisticated health conditions, and further to offer possibilities of building a smart healthcare and medical system. Additionally, continuous temperature mapping in flexible and stretchable formats opens up many other potential areas, such as artificially electronic skins and reflection of emotional changes...
May 26, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28547891/large-scale-gene-expression-reveals-different-adaptations-of-hyalopterus-persikonus-to-winter-and-summer-host-plants
#19
Na Cui, Peng-Cheng Yang, Kun Guo, Le Kang, Feng Cui
Host alternation, an obligatory seasonal shifting between host plants of distant genetic relationship, has had significant consequences for the diversification and success of the superfamily of aphids. However, the underlying molecular mechanism remains unclear. In this study, the molecular mechanism of host alternation was explored through a large-scale gene expression analysis of the mealy aphid Hyalopterus persikonus on winter and summer host plants. More than four times as many unigenes of the mealy aphid were significantly upregulated on summer host Phragmites australis than on winter host Rosaceae plants...
June 2017: Insect Science
https://www.readbyqxmd.com/read/28547530/reduced-cam-kinase-ii-and-cam-kinase-iv-activities-underlie-cognitive-deficits-in-nckx2-heterozygous-mice
#20
Shigeki Moriguchi, Satomi Kita, Yasushi Yabuki, Ryo Inagaki, Hisanao Izumi, Yuzuru Sasaki, Hideaki Tagashira, Kyoji Horie, Junji Takeda, Takahiro Iwamoto, Kohji Fukunaga
Among five members of the K(+)-dependent Na(+)/Ca(2+) exchanger (NCKX) family (NCKX1-5), only NCKX2 is highly expressed in mouse brain. NCKX2 in plasma membranes mediates cytosolic calcium excretion through electrogenic exchange of 4 Na(+) for 1 Ca(2+) and 1 K(+). Here, we observed significantly decreased levels of NCKX2 protein and mRNA in the CA1 region of APP23 mice, a model of Alzheimer's disease. We also found that, like APP23 mice, heterozygous NCKX2-mutant mice exhibit mildly impaired hippocampal LTP and memory acquisition, the latter based on novel object recognition and passive avoidance tasks...
May 25, 2017: Molecular Neurobiology
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