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https://www.readbyqxmd.com/read/28058637/efficient-screening-of-potential-cellulases-and-hemicellulases-produced-by-bosea-sp-fbzp-16-using-the-combination-of-enzyme-assays-and-genome-analysis
#1
Aicha Asma Houfani, Tomáš Větrovský, Petr Baldrian, Said Benallaoua
Identification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28031033/the-aceii-recombinant-trichoderma-reesei-qm9414-strains-with-enhanced-xylanase-production-and-its-applications-in-production-of-xylitol-from-tree-barks
#2
Lili Xiong, Ayyappa Kumar Sista Kameshwar, Xi Chen, Zhiyun Guo, Canquan Mao, Sanfeng Chen, Wensheng Qin
BACKGROUND: ACEII transcription factor plays a significant role in regulating the expression of cellulase and hemicellulase encoding genes. Apart from ACEII, transcription factors such as XYR1, CRE1, HAP2/3/5 complex and ACEI function in a coordinated pattern for regulating the gene expression of cellulases and hemicellulases. Studies have demonstrated that ACEII gene deletion results in decreased total cellulase and xylanase activities with reduced transcript levels of lignocellulolytic enzymes...
December 28, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/28003194/laci-transcriptional-regulatory-networks-in-clostridium-thermocellum-dsm1313
#3
Charlotte M Wilson, Dawn M Klingeman, Caleb Schlachter, Mustafa H Syed, Chia-Wei Wu, Adam M Guss, Steven D Brown
: Organisms regulate gene expression in response to the environment to coordinate metabolic reactions. Clostridium thermocellum expresses enzymes for both lignocellulose solubilization and its fermentation to produce ethanol. One LacI regulator termed GlyR3 in C. thermocellum ATCC27405 was previously identified as a repressor of neighboring genes with repression relieved by laminaribiose (a β-1,3 disaccharide). To better understand the three C. thermocellum LacI regulons, deletion mutants were constructed using the genetically tractable DSM1313 strain...
December 21, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27997079/optimization-of-enzymatic-hydrolysis-and-fermentation-conditions-for-improved-bioethanol-production-from-potato-peel-residues
#4
Imen Ben Taher, Patrick Fickers, Sofien Chniti, Mnasser Hassouna
The aim of this work was the optimization of the enzyme hydrolysis of potato peel residues (PPR) for bioethanol production. The process included a pretreatment step followed by an enzyme hydrolysis using crude enzyme system composed of cellulase, amylase and hemicellulase, produced by a mixed culture of Aspergillus niger and Trichoderma reesei. Hydrothermal, alkali and acid pretreatments were considered with regards to the enhancement of enzyme hydrolysis of potato peel residues. The obtained results showed that hydrothermal pretreatment lead to a higher enzyme hydrolysis yield compared to both acid and alkali pretreatments...
December 20, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27986827/potential-of-semiarid-soil-from-caatinga-biome-as-a-novel-source-for-mining-lignocellulose-degrading-enzymes
#5
Gileno Lacerda Júnior, Melline Noronha, Sanderson Tarciso de Sousa, Lucélia Cabral, Daniela Domingos, Mírian Sáber, Itamar de Melo, Valéria Oliveira
The litterfall is the major organic material deposited in soil of Brazilian Caatinga biome, thus providing the ideal conditions for plant biomass-degrading microorganisms to thrive. Herein, the phylogenetic composition and lignocellulose degrading capacity have been explored for the first time from a fosmid library dataset of Caatinga soil by sequence-based screening. A complex bacterial community dominated by Proteobacteria and Actinobacteria was unraveled. SEED subsystems-based annotations revealed a broad range of genes assigned to carbohydrate and aromatic compounds metabolism, indicating microbial ability to utilize plant-derived material...
December 15, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27936240/composting-like-conditions-are-more-efficient-for-enrichment-and-diversity-of-organisms-containing-cellulase-encoding-genes-than-submerged-cultures
#6
Senta Heiss-Blanquet, Françoise Fayolle-Guichard, Vincent Lombard, Agnès Hébert, Pedro M Coutinho, Alexis Groppi, Aurélien Barre, Bernard Henrissat
Cost-effective biofuel production from lignocellulosic biomass depends on efficient degradation of the plant cell wall. One of the major obstacles for the development of a cost-efficient process is the lack of resistance of currently used fungal enzymes to harsh conditions such as high temperature. Adapted, thermophilic microbial communities provide a huge reservoir of potentially interesting lignocellulose-degrading enzymes for improvement of the cellulose hydrolysis step. In order to identify such enzymes, a leaf and wood chip compost was enriched on a mixture of thermo-chemically pretreated wheat straw, poplar and Miscanthus under thermophile conditions, but in two different set-ups...
2016: PloS One
https://www.readbyqxmd.com/read/27919810/immobilization-of-indigenous-holocellulase-on-iron-oxide-fe2o3-nanoparticles-enhanced-hydrolysis-of-alkali-pretreated-paddy-straw
#7
Ajay Kumar, Surender Singh, Rameshwar Tiwari, Renu Goel, Lata Nain
The holocellulase from Aspergillus niger SH3 was characterized and found to contain 125 proteins including cellulases (26), hemicellulases (21), chitinases (10), esterases (6), amylases (4) and hypothetical protein (32). The crude enzyme was immobilized on five different nanoparticles (NPs) via physical adsorption and covalent coupling methods. The enzyme-nanoparticle complexes (ENC) were screened for protein binding, enzymatic activities and immobilization efficiency. Magnetic enzyme-nanoparticle complexes (MENC) showed higher immobilization efficiency (60-80%) for most of the enzymes...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27905924/current-knowledge-and-perspectives-of-paenibacillus-a-review
#8
REVIEW
Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman, Ze-Chun Yuan
Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via biological nitrogen fixation, phosphate solubilization, production of the phytohormone indole-3-acetic acid (IAA), and release of siderophores that enable iron acquisition. They can also offer protection against insect herbivores and phytopathogens, including bacteria, fungi, nematodes, and viruses...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27875311/single-binding-mode-integration-of-hemicellulose-degrading-enzymes-via-adaptor-scaffoldins-in-ruminococcus-flavefaciens-cellulosome
#9
Pedro Bule, Victor D Alves, André Leitão, Luís M A Ferreira, Edward A Bayer, Steven P Smith, Harry J Gilbert, Shabir Najmudin, Carlos M G A Fontes
The assembly of one of Nature's most elaborate multienzyme complexes, the cellulosome, results from the binding of enzyme-borne dockerins to reiterated cohesin domains located in a non-catalytic primary scaffoldin. Generally, dockerins present two similar cohesin-binding interfaces that support a dual binding mode. The dynamic integration of enzymes in cellulosomes, afforded by the dual binding mode, is believed to incorporate additional flexibility in highly populated multienzyme complexes. Ruminococcus flavefaciens, the primary degrader of plant structural carbohydrates in the rumen of mammals, uses a portfolio of more than 220 different dockerins to assemble the most intricate cellulosome known to date...
December 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27874287/impact-of-saccharomyces-cerevisiae-metabolites-produced-during-fermentation-on-bread-quality-parameters-a-review
#10
Mareile Heitmann, Emanuele Zannini, Elke Arendt
Although bread making with the use of Baker's yeast has a long tradition in human history, little attention has been paid to the connection between yeast addition and the final bread quality. Nowadays, bakers mainly use different flour additives such as enzymes (amylases, hemicellulases, and proteases) to change and improve dough properties and/or bread quality. Another strategy is the use of modified industrial Baker's yeast. To date, there is no yeast strain used in the baking industry, which is genetically modified, despite some studies demonstrating that the application of recombinant DNA technology is a possibility for improved strains suitable for baking...
November 22, 2016: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/27871321/biochemical-characterization-and-synergism-of-cellulolytic-enzyme-system-from-chaetomium-globosum-on-rice-straw-saccharification
#11
Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana, Verawat Champreda
BACKGROUND: Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their components is considered a promising approach. RESULTS: In this study, a lignocellulose-degrading enzyme system of Chaetomium globosum BCC5776 (CG-Cel) was characterized for its activity and proteomic profiles, and synergism with accessory enzymes. The highest cellulase productivity of 0...
November 21, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27825736/in-vitro-growth-and-cell-wall-degrading-enzyme-production-by-argentinean-isolates-of-macrophomina-phaseolina-the-causative-agent-of-charcoal-rot-in-corn
#12
Araceli M Ramos, Marcela Gally, Gala Szapiro, Tatiana Itzcovich, Maira Carabajal, Laura Levin
Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non-till practices. In this work, we explored the in vitro production of plant cell wall-degrading enzymes [pectinases (polygalacturonase and polymethylgalacturonase); cellulases (endoglucanase); hemicellulases (endoxylanase) and the ligninolytic enzyme laccase] by several Argentinean isolates of M...
October 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27765423/an-approach-for-extraction-of-kernel-oil-from-pinus-pumila-using-homogenate-circulating-ultrasound-in-combination-with-an-aqueous-enzymatic-process-and-evaluation-of-its-antioxidant-activity
#13
Fengli Chen, Qiang Zhang, Huiyan Gu, Lei Yang
In this study, a novel approach involving homogenate-circulating ultrasound in combination with aqueous enzymatic extraction (H-CUAEE) was developed for extraction of kernel oil from Pinus pumila. Following comparison of enzyme types and concentrations, an enzyme mixture consisting of cellulase, pectinase and hemicellulase (1:1:1, w/w/w) at a concentration of 2.5% was selected and applied for effective oil extraction and release. Several variables potentially influencing extraction yields, namely, homogenization time, incubation temperature, incubation time, mark-space ratio of ultrasound irradiation, ultrasound irradiation power, liquid-solid ratio, pH and stirring rate, were optimized by Plackett-Burman design...
November 4, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27744242/mycothermus-thermophilus-syn-scytalidium-thermophilum-repertoire-of-a-diverse-array-of-efficient-cellulases-and-hemicellulases-in-the-secretome-revealed
#14
Neha Basotra, Baljit Kaur, Marcos Di Falco, Adrian Tsang, Bhupinder Singh Chadha
Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium. The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied. Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present...
December 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27739446/penicillium-arizonense-a-new-genome-sequenced-fungal-species-reveals-a-high-chemical-diversity-in-secreted-metabolites
#15
Sietske Grijseels, Jens Christian Nielsen, Milica Randelovic, Jens Nielsen, Kristian Fog Nielsen, Mhairi Workman, Jens Christian Frisvad
A new soil-borne species belonging to the Penicillium section Canescentia is described, Penicillium arizonense sp. nov. (type strain CBS 141311(T) = IBT 12289(T)). The genome was sequenced and assembled into 33.7 Mb containing 12,502 predicted genes. A phylogenetic assessment based on marker genes confirmed the grouping of P. arizonense within section Canescentia. Compared to related species, P. arizonense proved to encode a high number of proteins involved in carbohydrate metabolism, in particular hemicellulases...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27722169/simultaneous-saccharification-and-fermentation-of-sugar-beet-pulp-for-efficient-bioethanol-production
#16
Joanna Berłowska, Katarzyna Pielech-Przybylska, Maria Balcerek, Urszula Dziekońska-Kubczak, Piotr Patelski, Piotr Dziugan, Dorota Kręgiel
Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27621450/localizing-gene-regulation-reveals-a-staggered-wood-decay-mechanism-for-the-brown-rot-fungus-postia-placenta
#17
Jiwei Zhang, Gerald N Presley, Kenneth E Hammel, Jae-San Ryu, Jon R Menke, Melania Figueroa, Dehong Hu, Galya Orr, Jonathan S Schilling
Wood-degrading brown rot fungi are essential recyclers of plant biomass in forest ecosystems. Their efficient cellulolytic systems, which have potential biotechnological applications, apparently depend on a combination of two mechanisms: lignocellulose oxidation (LOX) by reactive oxygen species (ROS) and polysaccharide hydrolysis by a limited set of glycoside hydrolases (GHs). Given that ROS are strongly oxidizing and nonselective, these two steps are likely segregated. A common hypothesis has been that brown rot fungi use a concentration gradient of chelated metal ions to confine ROS generation inside wood cell walls before enzymes can infiltrate...
September 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27618962/insight-into-enzymatic-degradation-of-corn-wheat-and-soybean-cell-wall-cellulose-using-quantitative-secretome-analysis-of-aspergillus-fumigatus
#18
Prakriti Sharma Ghimire, Haomiao Ouyang, Qian Wang, Yuanming Luo, Bo Shi, Jinghua Yang, Yang Lü, Cheng Jin
Lignocelluloses contained in animal forage cannot be digested by pigs or poultry with 100% efficiency. On contrary, Aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase encoding genes, suggesting that A. fumigatus is an efficient lignocellulose degrader. Hence the present study uses corn, wheat, or soybean as a sole carbon source to culture A. fumigatus under animal physiological condition to understand how cellulolytic enzymes work together to achieve an efficient degradation of lignocellulose...
December 2, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27607466/effects-of-different-substrates-on-lignocellulosic-enzyme-expression-enzyme-activity-substrate-utilization-and-biological-efficiency-of-pleurotus-eryngii
#19
Chunliang Xie, Li Yan, Wenbing Gong, Zuohua Zhu, Senwei Tan, Du Chen, Zhenxiu Hu, Yuande Peng
BACKGROUND/AIMS: Pleurotus eryngii is one of the most valued and delicious mushrooms which are commercially cultivated on various agro-wastes. How different substrates affect lignocellulosic biomass degradation, lignocellulosic enzyme production and biological efficiency in Pleurotus eryngii was unclear. METHODS AND RESULTS: In this report, Pleurotus eryngii was cultivated in substrates including ramie stalks, kenaf stalks, cottonseed hulls and bulrush stalks. The results showed that ramie stalks and kenaf stalks were found to best suitable to cultivate Pleurotus eryngii with the biological efficiency achieved at 55% and 57%, respectively...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27602056/comparison-of-saccharification-and-fermentation-of-steam-exploded-rice-straw-and-rice-husk
#20
Ian P Wood, Huong-Giang Cao, Long Tran, Nicola Cook, Peter Ryden, David R Wilson, Graham K Moates, Samuel R A Collins, Adam Elliston, Keith W Waldron
BACKGROUND: Rice cultivation produces two waste streams, straw and husk, which could be exploited more effectively. Chemical pretreatment studies using rice residues have largely focussed on straw exploitation alone, and often at low substrate concentrations. Moreover, it is currently not known how rice husk, the more recalcitrant residue, responds to steam explosion without the addition of chemicals. RESULTS: The aim of this study has been to systematically compare the effects of steam explosion severity on the enzymatic saccharification and simultaneous saccharification and fermentation of rice straw and husk produced from a variety widely grown in Vietnam (Oryza sativa, cv...
2016: Biotechnology for Biofuels
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