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https://www.readbyqxmd.com/read/28920414/metagenomic-analysis-of-bacterial-community-structure-and-diversity-of-lignocellulolytic-bacteria-in-vietnamese-native-goat-rumen
#1
Huyen Thi Do, Khoa Trong Dao, Viet Khanh Hoang Nguyen, Giang Ngoc Le, Phuong Thi Mai Nguyen, Lam Tung Le, Nguyet Thu Phung, Nico van Straalen, Dick Roelofs, Hai Nam Truong
Objective: In a previous study, analysis of Illumina sequenced metagenomic DNA data of bacteria in Vietnamese goats' rumen showed a high diversity of putative lignocellulolytic genes. In this study, taxonomy speculation of microbial community and lignocellulolytic bacteria population in the rumen was conducted to elucidate a role of bacterial structure for effective degradation of plant materials. Methods: The metagenomic data had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm and the National Center for Biotechnology Information (NCBI) non-redundant (NR) sequence database...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28912831/genome-sequencing-and-transcriptome-analysis-of-trichoderma-reesei-qm9978-strain-reveals-a-distal-chromosome-translocation-to-be-responsible-for-loss-of-vib1-expression-and-loss-of-cellulase-induction
#2
Christa Ivanova, Jonas Ramoni, Thiziri Aouam, Alexa Frischmann, Bernhard Seiboth, Scott E Baker, Stéphane Le Crom, Sophie Lemoine, Antoine Margeot, Frédérique Bidard
BACKGROUND: The hydrolysis of biomass to simple sugars used for the production of biofuels in biorefineries requires the action of cellulolytic enzyme mixtures. During the last 50 years, the ascomycete Trichoderma reesei, the main source of industrial cellulase and hemicellulase cocktails, has been subjected to several rounds of classical mutagenesis with the aim to obtain higher production levels. During these random genetic events, strains unable to produce cellulases were generated...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28900905/biodegradation-of-ramie-stalk-by-flammulina-velutipes-mushroom-production-and-substrate-utilization
#3
Chunliang Xie, Wenbing Gong, Li Yan, Zuohua Zhu, Zhenxiu Hu, Yuande Peng
In the textile industry, ramie stalk is byproducts with a low economic value. The potential use of this leftover as a substrate ingredient for Flammulina velutipes (F. velutipe) cultivation was evaluated. The degradation and utilization of ramie stalk by F. velutipes was evaluated through mushroom production, lignocelluloses degradation and lignocellulolytic enzymes activity. The best substrate mixture for F. velutipes cultivation comprised 50% ramie stalk, 20% cottonseed hulls, 25% wheat bran, 4% cornstarch and 2% CaCO3...
September 12, 2017: AMB Express
https://www.readbyqxmd.com/read/28895359/-establishment-and-optimization-of-systems-for-protoplasts-isolation-of-soybean-and-chickpea-that-used-in-subcellular-location
#4
Yingjie Shu, Liyan Huang, Ming Chen, Yuan Tao, Zhankui Wang, Hao Ma
Young leaves of Kabuli chickpea as well as soybean Xiangdou No.3, which are the current plants that studied in our laboratory were selected as materials. Effects on protoplasts yield and survival rate of different enzyme combination, concentration of D-Mannitol in enzyme combinations, pH of enzyme combinations and enzymolysis time are detected. The results showed that, the best condition for Xiangdou No.3 leaf protoplasts isolation is to rotate the cut materials for 6 hours in enyzme solution under temperature of 27 ℃ and rotate speed of 45 r/min for 6 h...
June 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28859626/isolation-of-ripening-related-genes-from-ethylene-1-mcp-treated-papaya-through-rna-seq
#5
Yan Hong Shen, Bing Guo Lu, Li Feng, Fei Ying Yang, Jiao Jiao Geng, Ray Ming, Xiao Jing Chen
BACKGROUND: Since papaya is a typical climacteric fruit, exogenous ethylene (ETH) applications can induce premature and quicker ripening, while 1-methylcyclopropene (1-MCP) slows down the ripening processes. Differential gene expression in ETH or 1-MCP-treated papaya fruits accounts for the ripening processes. To isolate the key ripening-related genes and better understand fruit ripening mechanisms, transcriptomes of ETH or 1-MCP-treated, and non-treated (Control Group, CG) papaya fruits were sequenced using Illumina Hiseq2500...
August 31, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28801839/biotransformation-with-cellulase-hemicellulase-and-yarrowia-lipolytica-boosts-health-benefits-of-okara
#6
Weng Chan Vong, Xin Ying Lim, Shao-Quan Liu
Okara (soybean residue) is a highly perishable food processing by-product from soymilk and tofu manufacture. It contains a large proportion of insoluble dietary fibre (40-60% on a dry basis), as well as digestion-resistant proteins, trypsin inhibitors and phytic acid. These factors contribute lead to the under-utilisation of okara. To improve the overall nutritional quality of okara, sequential saccharification of okara by Celluclast® 1.5L (cellulase) or Viscozyme® L (cellulase and hemicellulase) and fermentation by the yeast Yarrowia lipolytica were performed...
August 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28764108/cellulase-and-xylanase-production-at-pilot-scale-by-solid-state-fermentation-from-coffee-husk-using-specialized-consortia-the-consistency-of-the-process-and-the-microbial-communities-involved
#7
Alejandra Cerda, Laura Mejías, Teresa Gea, Antoni Sánchez
Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale...
July 16, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28755483/constitutive-expression-of-chimeric-transcription-factors-enables-cellulase-synthesis-under-non-inducing-conditions-in-penicillium-oxalicum
#8
Liwei Gao, Chengqiang Xia, Jiadi Xu, Zhonghai Li, Lele Yu, Guodong Liu, Xin Song, Yinbo Qu
Industrial production of cellulase by filamentous fungi is largely dependent on cellulose, which serves as a natural inducer of cellulase expression. However, insoluble cellulose is unfavorable to submerged fermentation and thus limits the production level of cellulase. The possibility of cellulase production under non-inducing conditions is explored in Penicillium oxalicum by overexpressing two chimeric transcription factors. The chimeric transcription factors contain the DNA binding domain of cellulase transcriptional activator ClrB linked to the C-terminal sequences of XlnR(A871V) , a constitutively active mutant of hemicellulase transcriptional activator...
July 29, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28704184/enzymatic-hydrolysis-of-water-extractable-polysaccharides-from-leaves-of-plantago-major-l
#9
Paolina K Lukova, Diana P Karcheva-Bahchevanska, Veselin P Bivolarski, Rumen D Mladenov, Ilia N Iliev, Mariana M Nikolova
BACKGROUND: Plantago major L. leaves have been used for centuries by the traditional medicine in the treatment of infectious disorders of the respiratory, urinary and digestive tracts. Researchers have reported that hot water extracts of Plantago major possess a broad-spectrum of anticancer, antioxidant and antiviral activities, as well as activities which modulate cell-mediated immunity. Their beneficial properties may be due to the significant content of polysaccharides. The polysaccharides that have been isolated from the leaves of Plantago major L...
June 1, 2017: Folia Medica
https://www.readbyqxmd.com/read/28688047/assessment-of-antioxidant-and-antimicrobial-properties-of-lignin-from-corn-stover-residue-pretreated-with-low-moisture-anhydrous-ammonia-and-enzymatic-hydrolysis-process
#10
Mingming Guo, Tony Jin, Nhuan P Nghiem, Xuetong Fan, Phoebe X Qi, Chan Ho Jang, Lingxiao Shao, Changqing Wu
Lignin accounts for 15-35% of dry biomass materials. Therefore, developing value-added co-products from lignin residues is increasingly important to improve the economic viability of biofuel production from biomass resources. The main objective of this work was to study the lignin extracts from corn stover residue obtained from a new and improved process for bioethanol production. Extraction conditions that favored high lignin yield were optimized, and antioxidant and antimicrobial activities of the resulting lignin were investigated...
July 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28666924/role-of-carbon-source-in-the-shift-from-oxidative-to-hydrolytic-wood-decomposition-by-postia-placenta
#11
Jiwei Zhang, Jonathan S Schilling
Brown rot fungi initiate wood decay using oxidative pretreatments to improve access for cellulolytic enzymes. These pretreatments are incompatible with enzymes, and we recently showed that Postia placenta overcomes this issue by delaying glycoside hydrolase (GH) gene upregulation briefly (<48h) until expression of oxidoreductases (ORs) is repressed. This implies an inducible cellulase system rather than a constitutive system, as often reported, and it remains unclear what cues this transition. To address this, we grew P...
June 27, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28660456/sorghum-husk-biomass-as-a-potential-substrate-for-production-of-cellulolytic-and-xylanolytic-enzymes-by-nocardiopsis-sp-knu
#12
Siddheshwar D Kshirsagar, Bhumika N Bhalkar, Pankajkumar R Waghmare, Ganesh D Saratale, Rijuta G Saratale, Sanjay P Govindwar
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28648256/a-comparative-analysis-of-pretreatment-strategies-on-the-properties-and-hydrolysis-of-brewers-spent-grain
#13
Rajeev Ravindran, Swarna Jaiswal, Nissreen Abu-Ghannam, Amit K Jaiswal
In this study, brewer's spent grain (BSG) was subjected to a range pretreatments to study the effect on reducing sugar yield. Glucose and xylose were found to be the predominant sugars in BSG. Brewers spent grain was high in cellulose (19.21g/100g of BSG) and lignin content (30.84g/100g of BSG). Microwave assisted alkali (MAA) pretreatment was found to be the most effective pretreatment for BSG, where the pretreatment was conducted at 400W for 60s. A maximum reducing yield was observed with high biomass loading (1g/10ml), cellulase (158...
June 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28634326/a-chrysoporthe-cubensis-enzyme-cocktail-produced-from-a-low-cost-carbon-source-with-high-biomass-hydrolysis-efficiency
#14
Thiago Rodrigues Dutra, Valéria Monteze Guimarães, Ednilson Mascarenhas Varela, Lílian da Silva Fialho, Adriane Maria Ferreira Milagres, Daniel Luciano Falkoski, José Cola Zanuncio, Sebastião Tavares de Rezende
Low cost and high efficiency cellulolytic cocktails can consolidate lignocellulosic ethanol technologies. Sugarcane bagasse (SCB) is a low cost agro-industrial residue, and its use as a carbon source can reduce the costs of fungi cultivation for enzyme production. Chrysoporthe cubensis grown under solid state fermentation (SSF) with wheat bran has potential to produce efficient enzymatic extracts for SCB saccharification. This fungus was grown under submersed fermentation (SmF) and SSF with in natura SCB, pretreated with acid or alkali and with others carbon sources...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28559622/the-effects-of-certain-enzymes-on-the-rheology-of-dough-and-the-quality-characteristics-of-bread-prepared-from-wheat-meal
#15
Burak Altınel, S Sezgin Ünal
This study was carried out to evaluate the effects of amyloglucosidase, glucose oxidase, hemicellulase (mainly consist of endo-1,4-β-xylanase), cellulase, lipase, and the combination of phospholipase and hemicellulase (phospholipase + hemicellulase) on the extensographic properties of dough and the quality characteristics of bread prepared from wheat meal. The enzymes were added separately in two different amounts. The addition of glucose oxidase (at 0.0003-0.001%) caused a significant decrease in the resistance to extension, ratio of resistance to extensibility and energy values of the wheat meal dough compared with the control dough...
May 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28556365/identification-and-expression-profiling-of-novel-plant-cell-wall-degrading-enzymes-from-a-destructive-pest-of-palm-trees-rhynchophorus-ferrugineus
#16
B Antony, J Johny, S A Aldosari, M M Abdelazim
Plant cell wall degrading enzymes (PCWDEs) from insects were recently identified as a multigene family of proteins that consist primarily of glycoside hydrolases (GHs) and carbohydrate esterases (CEs) and play essential roles in the degradation of the cellulose/hemicellulose/pectin network in the invaded host plant. Here we applied transcriptomic and degenerate PCR approaches to identify the PCWDEs from a destructive pest of palm trees, Rhynchophorus ferrugineus, followed by a gut-specific and stage-specific differential expression analysis...
August 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28525726/characterization-of-an-ionic-liquid-tolerant-%C3%AE-xylosidase-from-a-marine-derived-fungal-endophyte
#17
Anindita Sengupta, Angela Zabala, Si Yu Tan, Arthur Broadstock, T S Suryanarayanan, Venkat Gopalan
Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment due to their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates development of a cellulases and hemicellulases that can tolerate ~20% (w/v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28483534/comparative-secretomic-analysis-of-lignocellulose-degradation-by-lentinula-edodes-grown-on-microcrystalline-cellulose-lignosulfonate-and-glucose
#18
Yingli Cai, Yuhua Gong, Wei Liu, Yue Hu, Lianfu Chen, Lianlian Yan, Yan Zhou, Yinbing Bian
Lentinula edodes has the potential to degrade woody and nonwoody lignocellulosic biomass. However, the mechanism of lignocellulose degradation by L. edodes is unclear. The aim of this work is to explore the profiling of soluble secreted proteins involved in lignocellulose degradation in L. edodes. For that, we compared the secretomes of L. edodes grown on microcrystalline cellulose, cellulose with lignosulfonate and glucose. Based on nanoliquid chromatography coupled with tandem mass spectrometry of whole-protein hydrolysate, 230 proteins were identified...
June 23, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28454712/transcriptome-based-analysis-of-the-saprophytic-fungus-abortiporus-biennis-response-to-oxalic-acid
#19
Marcin Grąz, Anna Jarosz-Wilkołazka, Grzegorz Janusz, Andrzej Mazur, Jerzy Wielbo, Piotr Koper, Kamil Żebracki, Agnieszka Kubik-Komar
In this study, the transcriptomic-based response of the white rot fungus Abortiporus biennis to oxalic acid induction was reported. The whole transcriptome of A. biennis was analysed using the RNA-based sequencing technology and Solid 5500 platform. De novo assembly of reads generated 37,719 contigs. A molecular function for 26,280 unique transcripts was assigned. The analysis of the A. biennis transcriptome predicted 635 hypothetical open reading frames encoding carbohydrate active enzymes distributed in 122 families...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28432102/two-distinct-%C3%AE-l-arabinofuranosidases-in-caldicellulosiruptor-species-drive-degradation-of-arabinose-based-polysaccharides
#20
Mohammad Abu Saleh, Wen-Jie Han, Ming Lu, Bing Wang, Huayue Li, Robert M Kelly, Fu-Li Li
Species in the extremely thermophilic genus Caldicellulosiruptor can degrade unpretreated plant biomass through the action of multimodular glycoside hydrolases. To date, most focus with these bacteria has been on hydrolysis of glucans and xylans, while the biodegradation mechanism for arabinose-based polysaccharides remains unclear. Here, putative α-l-arabinofuranosidases (AbFs) were identified in Caldicellulosiruptor species by homology to less-thermophilic versions of these enzymes. From this screen, an extracellular XynF was determined to be a key factor in hydrolyzing α-1,2-, α-1,3-, and α-1,5-l-arabinofuranosyl residues of arabinose-based polysaccharides...
July 1, 2017: Applied and Environmental Microbiology
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