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https://www.readbyqxmd.com/read/27919810/immobilization-of-indigenous-holocellulase-on-iron-oxide-fe2o3-nanoparticles-enhanced-hydrolysis-of-alkali-pretreated-paddy-straw
#1
Ajay Kumar, Surender Singh, Rameshwar Tiwari, Renu Goel, Lata Nain
The holocellulase from Aspergillus niger SH3 was characterized and found to contain 125 proteins including cellulases (26), hemicellulases (21), chitinases (10), esterases (6), amylases (4) and hypothetical protein (32). The crude enzyme was immobilized on five different nanoparticles (NPs) via physical adsorption and covalent coupling methods. The enzyme-nanoparticle complexes (ENC) were screened for protein binding, enzymatic activities and immobilization efficiency. Magnetic enzyme-nanoparticle complexes (MENC) formulation showed higher immobilization efficiency (60- 80%) for most of the enzymes...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27905924/current-knowledge-and-perspectives-of-paenibacillus-a-review
#2
REVIEW
Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman, Ze-Chun Yuan
Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via biological nitrogen fixation, phosphate solubilization, production of the phytohormone indole-3-acetic acid (IAA), and release of siderophores that enable iron acquisition. They can also offer protection against insect herbivores and phytopathogens, including bacteria, fungi, nematodes, and viruses...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27875311/single-binding-mode-integration-of-hemicellulose-degrading-enzymes-via-adaptor-scaffoldins-in-ruminococcus-flavefaciens-cellulosome
#3
Pedro Bule, Victor Diogo Alves, André Leitão, Luis M A Ferreira, Edward A Bayer, Steven P Smith, Harry J Gilbert, Shabir Najmudin, Carlos M G A Fontes
The assembly of one of Nature most elaborate multi-enzyme complexes, the cellulosome, results from the binding of enzyme-borne dockerins to reiterated cohesin domains located in a non-catalytic primary scaffoldin. Generally, dockerins present two similar cohesin binding interfaces that support a dual binding mode. The dynamic integration of enzymes in cellulosomes, afforded by the dual binding mode, is believed to incorporate additional flexibility in highly populated multi-enzyme complexes. Ruminococcus flavefaciens, the primary degrader of plant structural carbohydrates in the rumen of mammals, uses a portfolio of more than 220 different enzymes to assemble the most intricate cellulosome known to date...
November 14, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27874287/impact-of-saccharomyces-cerevisiae-metabolites-produced-during-fermentation-on-bread-quality-parameters-a-review
#4
Mareile Heitmann, Emanuele Zannini, Elke Arendt
Although bread making with the use of Baker's yeast has a long tradition in human history, little attention has been paid to the connection between yeast addition and the final bread quality. Nowadays, bakers mainly use different flour additives such as enzymes (amylases, hemicellulases, and proteases) to change and improve dough properties and/or bread quality. Another strategy is the use of modified industrial Baker's yeast. To date, there is no yeast strain used in the baking industry, which is genetically modified, despite some studies demonstrating that the application of recombinant DNA technology is a possibility for improved strains suitable for baking...
November 22, 2016: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/27871321/biochemical-characterization-and-synergism-of-cellulolytic-enzyme-system-from-chaetomium-globosum-on-rice-straw-saccharification
#5
Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana, Verawat Champreda
BACKGROUND: Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their components is considered a promising approach. RESULTS: In this study, a lignocellulose-degrading enzyme system of Chaetomium globosum BCC5776 (CG-Cel) was characterized for its activity and proteomic profiles, and synergism with accessory enzymes. The highest cellulase productivity of 0...
November 21, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27825736/in-vitro-growth-and-cell-wall-degrading-enzyme-production-by-argentinean-isolates-of-macrophomina-phaseolina-the-causative-agent-of-charcoal-rot-in-corn
#6
Araceli M Ramos, Marcela Gally, Gala Szapiro, Tatiana Itzcovich, Maira Carabajal, Laura Levin
Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non-till practices. In this work, we explored the in vitro production of plant cell wall-degrading enzymes [pectinases (polygalacturonase and polymethylgalacturonase); cellulases (endoglucanase); hemicellulases (endoxylanase) and the ligninolytic enzyme laccase] by several Argentinean isolates of M...
November 5, 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27765423/an-approach-for-extraction-of-kernel-oil-from-pinus-pumila-using-homogenate-circulating-ultrasound-in-combination-with-an-aqueous-enzymatic-process-and-evaluation-of-its-antioxidant-activity
#7
Fengli Chen, Qiang Zhang, Huiyan Gu, Lei Yang
In this study, a novel approach involving homogenate-circulating ultrasound in combination with aqueous enzymatic extraction (H-CUAEE) was developed for extraction of kernel oil from Pinus pumila. Following comparison of enzyme types and concentrations, an enzyme mixture consisting of cellulase, pectinase and hemicellulase (1:1:1, w/w/w) at a concentration of 2.5% was selected and applied for effective oil extraction and release. Several variables potentially influencing extraction yields, namely, homogenization time, incubation temperature, incubation time, mark-space ratio of ultrasound irradiation, ultrasound irradiation power, liquid-solid ratio, pH and stirring rate, were optimized by Plackett-Burman design...
October 14, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27744242/mycothermus-thermophilus-syn-scytalidium-thermophilum-repertoire-of-a-diverse-array-of-efficient-cellulases-and-hemicellulases-in-the-secretome-revealed
#8
Neha Basotra, Baljit Kaur, Marcos Di Falco, Adrian Tsang, Bhupinder Singh Chadha
Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium. The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied. Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present...
October 8, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27739446/penicillium-arizonense-a-new-genome-sequenced-fungal-species-reveals-a-high-chemical-diversity-in-secreted-metabolites
#9
Sietske Grijseels, Jens Christian Nielsen, Milica Randelovic, Jens Nielsen, Kristian Fog Nielsen, Mhairi Workman, Jens Christian Frisvad
A new soil-borne species belonging to the Penicillium section Canescentia is described, Penicillium arizonense sp. nov. (type strain CBS 141311(T) = IBT 12289(T)). The genome was sequenced and assembled into 33.7 Mb containing 12,502 predicted genes. A phylogenetic assessment based on marker genes confirmed the grouping of P. arizonense within section Canescentia. Compared to related species, P. arizonense proved to encode a high number of proteins involved in carbohydrate metabolism, in particular hemicellulases...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27722169/simultaneous-saccharification-and-fermentation-of-sugar-beet-pulp-for-efficient-bioethanol-production
#10
Joanna Berłowska, Katarzyna Pielech-Przybylska, Maria Balcerek, Urszula Dziekońska-Kubczak, Piotr Patelski, Piotr Dziugan, Dorota Kręgiel
Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27621450/localizing-gene-regulation-reveals-a-staggered-wood-decay-mechanism-for-the-brown-rot-fungus-postia-placenta
#11
Jiwei Zhang, Gerald N Presley, Kenneth E Hammel, Jae-San Ryu, Jon R Menke, Melania Figueroa, Dehong Hu, Galya Orr, Jonathan S Schilling
Wood-degrading brown rot fungi are essential recyclers of plant biomass in forest ecosystems. Their efficient cellulolytic systems, which have potential biotechnological applications, apparently depend on a combination of two mechanisms: lignocellulose oxidation (LOX) by reactive oxygen species (ROS) and polysaccharide hydrolysis by a limited set of glycoside hydrolases (GHs). Given that ROS are strongly oxidizing and nonselective, these two steps are likely segregated. A common hypothesis has been that brown rot fungi use a concentration gradient of chelated metal ions to confine ROS generation inside wood cell walls before enzymes can infiltrate...
September 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27618962/insight-into-enzymatic-degradation-of-corn-wheat-and-soybean-cell-wall-cellulose-using-quantitative-secretome-analysis-of-aspergillus-fumigatus
#12
Prakriti Sharma Ghimire, Haomiao Ouyang, Qian Wang, Yuanming Luo, Bo Shi, Jinghua Yang, Yang Lü, Cheng Jin
Lignocelluloses contained in animal forage cannot be digested by pigs or poultry with 100% efficiency. On contrary, Aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase encoding genes, suggesting that A. fumigatus is an efficient lignocellulose degrader. Hence the present study uses corn, wheat, or soybean as a sole carbon source to culture A. fumigatus under animal physiological condition to understand how cellulolytic enzymes work together to achieve an efficient degradation of lignocellulose...
October 10, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27607466/effects-of-different-substrates-on-lignocellulosic-enzyme-expression-enzyme-activity-substrate-utilization-and-biological-efficiency-of-pleurotus-eryngii
#13
Chunliang Xie, Li Yan, Wenbing Gong, Zuohua Zhu, Senwei Tan, Du Chen, Zhenxiu Hu, Yuande Peng
BACKGROUND/AIMS: Pleurotus eryngii is one of the most valued and delicious mushrooms which are commercially cultivated on various agro-wastes. How different substrates affect lignocellulosic biomass degradation, lignocellulosic enzyme production and biological efficiency in Pleurotus eryngii was unclear. METHODS AND RESULTS: In this report, Pleurotus eryngii was cultivated in substrates including ramie stalks, kenaf stalks, cottonseed hulls and bulrush stalks. The results showed that ramie stalks and kenaf stalks were found to best suitable to cultivate Pleurotus eryngii with the biological efficiency achieved at 55% and 57%, respectively...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27602056/comparison-of-saccharification-and-fermentation-of-steam-exploded-rice-straw-and-rice-husk
#14
Ian P Wood, Huong-Giang Cao, Long Tran, Nicola Cook, Peter Ryden, David R Wilson, Graham K Moates, Samuel R A Collins, Adam Elliston, Keith W Waldron
BACKGROUND: Rice cultivation produces two waste streams, straw and husk, which could be exploited more effectively. Chemical pretreatment studies using rice residues have largely focussed on straw exploitation alone, and often at low substrate concentrations. Moreover, it is currently not known how rice husk, the more recalcitrant residue, responds to steam explosion without the addition of chemicals. RESULTS: The aim of this study has been to systematically compare the effects of steam explosion severity on the enzymatic saccharification and simultaneous saccharification and fermentation of rice straw and husk produced from a variety widely grown in Vietnam (Oryza sativa, cv...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27588830/mcma-dependent-and-independent-regulatory-systems-governing-expression-of-clrb-regulated-cellulase-and-hemicellulase-genes-in-aspergillus-nidulans
#15
Nuo Li, Emi Kunitake, Miki Aoyama, Masahiro Ogawa, Kyoko Kanamaru, Makoto Kimura, Yasuji Koyama, Tetsuo Kobayashi
Fungal cellulolytic and hemicellulolytic enzymes are promising tools for industrial hydrolysis of cellulosic biomass; however, the regulatory network underlying their production is not well understood. The recent discovery of the transcriptional activators ClrB and McmA in Aspergillus nidulans implied a novel regulatory mechanism driven by their interaction, experimental evidence for which was obtained from transcriptional and DNA-binding analyses in this study. It was found that ClrB was essential for induced expression of all the genes examined in this study, while McmA dependency of their expression was gene-dependent...
September 2, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27558133/effects-of-brevibacillus-brevis-fjat-1501-bpa-on-growth-performance-faecal-microflora-faecal-enzyme-activities-and-blood-parameters-of-weaned-piglets
#16
Jianmei Che, Shaowen Ye, Bo Liu, Yuanyuan Deng, Qianqian Chen, Cibin Ge, Guohong Liu, Jieping Wang
A feeding expriment was performed to investigate the effects of dietary supplementation with Brevibacillus brevis FJAT-1501-BPA fermentation on the growth performance, faecal microflora, faecal enzyme activities and blood parameters of weaned piglets. A total of 150 weaned piglets were randomly assigned to different treatments groups, which were fed the same basic diet supplemented with 10, 1, 0.1, 0.01 and 0 % B. brevis FJAT-1501-BPA fermentation. The results showed that a diet supplemented with 10 % B. brevis FJAT-1501-BPA fermentation could significantly increase the final body weight (P < 0...
August 24, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27506519/the-use-of-t-dna-insertional-mutagenesis-to-improve-cellulase-production-by-the-thermophilic-fungus-humicola-insolens-y1
#17
Xinxin Xu, Jinyang Li, Pengjun Shi, Wangli Ji, Bo Liu, Yuhong Zhang, Bin Yao, Yunliu Fan, Wei Zhang
Humicola insolens is an excellent producer of pH-neutral active, thermostable cellulases that find many industrial applications. In the present study, we developed an efficient Agrobacterium tumefaciens-mediated transformation system for H. insolens. We transformed plasmids carrying the promoter of the glyceraldehyde-3-phosphate dehydrogenase gene of H. insolens driving the transcription of genes encoding neomycin phosphotransferase, hygromycin B phosphotransferase, and enhanced green fluorescent protein. We optimized transformation efficiency to obtain over 300 transformants/10(6) conidia...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27434633/comparative-analysis-of-secretome-profiles-of-manganese-ii-oxidizing-ascomycete-fungi
#18
Carolyn A Zeiner, Samuel O Purvine, Erika M Zink, Ljiljana Paša-Tolić, Dominique L Chaput, Sajeet Haridas, Si Wu, Kurt LaButti, Igor V Grigoriev, Bernard Henrissat, Cara M Santelli, Colleen M Hansel
Fungal secretomes contain a wide range of hydrolytic and oxidative enzymes, including cellulases, hemicellulases, pectinases, and lignin-degrading accessory enzymes, that synergistically drive litter decomposition in the environment. While secretome studies of model organisms such as Phanerochaete chrysosporium and Aspergillus species have greatly expanded our knowledge of these enzymes, few have extended secretome characterization to environmental isolates or conducted side-by-side comparisons of diverse species...
2016: PloS One
https://www.readbyqxmd.com/read/27419196/secretome-data-from-trichoderma-reesei-and-aspergillus-niger-cultivated-in-submerged-and-sequential-fermentation-methods
#19
Camila Florencio, Fernanda M Cunha, Alberto C Badino, Cristiane S Farinas, Eduardo Ximenes, Michael R Ladisch
The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical information to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, esterase, etc...
September 2016: Data in Brief
https://www.readbyqxmd.com/read/27405320/genomic-characterization-of-plant-cell-wall-degrading-enzymes-and-in-silico-analysis-of-xylanses-and-polygalacturonases-of-fusarium-virguliforme
#20
Hao-Xun Chang, Craig R Yendrek, Gustavo Caetano-Anolles, Glen L Hartman
BACKGROUND: Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interact with fungal glycoside hydrolase (GH)11-type xylanases or GH28-type polygalacturonase (PG) have been shown to enhance disease resistance. However, many plant pathogenic Fusarium species were reported to escape PIPs inhibition...
2016: BMC Microbiology
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