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Gastric stem cell

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https://www.readbyqxmd.com/read/28446252/ring-finger-protein-43-associates-with-gastric-cancer-progression-and-attenuates-the-stemness-of-gastric-cancer-stem-like-cells-via-the-wnt-%C3%AE-catenin-signaling-pathway
#1
Yunhe Gao, Aizhen Cai, Hongqing Xi, Jiyang Li, Wei Xu, Yanmei Zhang, Kecheng Zhang, Jianxin Cui, Xiaosong Wu, Bo Wei, Lin Chen
BACKGROUND: Ring finger protein 43 (RNF43) is a member of the transmembrane E3 ubiquitin ligase family that was originally found in stem cells and plays important roles in tumor formation and progression. Our previous study indicated that RNF43 might be a tumor suppressor protein in gastric cancer. Given its antagonistic relationship with leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5), one of the gastric cancer stem cell markers, investigation of the potential role of RNF43 in gastric stem cancer cells is necessary...
April 26, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28440445/mir%C3%A2-196a%C3%A2-5p-modulates-gastric-cancer-stem-cell-characteristics-by-targeting-smad4
#2
Yunzhi Pan, Xiong Shu, Lichao Sun, Long Yu, Lixin Sun, Zhihua Yang, Yuliang Ran
Cancer stem cells (CSCs) are undifferentiated cancer cells with a high tumorigenic activity, the ability to undergo self-renewal, and a multilineage differentiation potential. Clinical evidence suggests that CSCs in a tumor mass are the cellular determinants to promote cancer invasion and metastasis. MicroRNAs (miRNAs) have emerged as important modulators of cancer stem cell characteristics. Unveiling the candidate miRNAs that regulate CSCs may provide novel therapeutic targets against cancer. We analyzed the miRNA expression profiles regulating the cancer stem-like cell characteristics in gastric cancer...
April 19, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28430578/involvement-of-polo-like-kinase-1-plk1-in-quiescence-regulation-of-cancer-stem-like-cells-of-the-gastric-cancer-cell-lines
#3
Lin Zhu, Sheng Xing, Li Zhang, Jian-Min Yu, Cheng Lin, Wei-Jun Yang
Cancer stem cells (CSCs) have been hypothesized to initiate tumor growth and be resistant to chemoradiotherapy, and these processes appear to be closely related to CSC quiescence. Here, a CSC-like cell population with a high level of CD44 expression was obtained from the human gastric cancer cell lines MKN45 and MKN74. Using a PKH26-labeling retention assay, quiescent CSC-like cells with low levels of Ki67 and PCNA expression were found in spheres formed in serum-free medium, and exhibited resistance to drug and radiation treatments...
April 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28429101/human-circulating-and-tissue-gastric-cancer-stem-cells-display-distinct-epithelial-mesenchymal-features-and-behaviors
#4
Shengliang Zhang, Yanna Shang, Tie Chen, Xin Zhou, Wengtong Meng, Chuanwen Fan, Ran Lu, Qiaorong Huang, Xue Li, Xu Hong, Zongguang Zhou, Jiankun Hu, Xianming Mo
INTRODUCTION: Metastasis is a leading cause of cancer-related-deaths worldwide. Recently, cancer stem cells (CSCs) have been believed to be responsible for tumor initiation and metastasis, but till now, difference of cellular features and behaviors between CSCs from tumor tissues (TCSCs) and circulation (CCSCs) remains largely unknown, which hinders the progression of targeted therapies for metastasis. METHODS AND RESULTS: Here, we provide the features of circulating gastric cancer stem cells (CGCSCs) isolated from human gastric adenocarcinoma...
April 20, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28428713/microrna-145-exerts-tumor-suppressive-and-chemo-resistance-lowering-effects-by-targeting-cd44-in-gastric-cancer
#5
Jian-Feng Zeng, Xiao-Qiu Ma, Lin-Pei Wang, Wei Wang
AIM: To determine the potential roles of CD4 and microRNA (miR)-145 in gastric cancer. METHODS: The levels of CD44 and miR-145 were determined in gastric cancer cells. Quantitative real-time polymerase chain reaction was used to measure to the level of CD44 mRNA. A luciferase reporter assay and western blotting were performed to examine the effect of miR-145 on CD44 expression. Tumor sphere and MTT assays were carried out to evaluate the self-renewal and chemo-resistance properties of gastric cancer cells...
April 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28420890/endoscopic-manifestations-and-clinical-characteristics-of-cytomegalovirus-infection-in-the-upper-gastrointestinal-tract
#6
Masaya Iwamuro, Eisei Kondo, Takehiro Tanaka, Hideharu Hagiya, Seiji Kawano, Yoshiro Kawahara, Fumio Otsuka, Hiroyuki Okada
We retrospectively analyzed the cases of 14 patients (9 women, 5 men, mean age: 51.6 years) with cytomegalovirus (CMV) involvement in the esophagus, stomach, and/or duodenum diagnosed at a single center, to determine their endoscopic features and clinical backgrounds. Thirteen patients (92.9%) had hematologic disease; the other had rheumatoid arthritis. Of the former, 12 patients underwent allogeneic hematopoietic stem cell transplantation, and 9 of these patients had graft-versus-host disease (GVHD) before undergoing esophagogastroduodenoscopy (EGD)...
April 2017: Acta Medica Okayama
https://www.readbyqxmd.com/read/28415792/a-cd44-specific-peptide-rp-1-exhibits-capacities-of-assisting-diagnosis-and-predicting-prognosis-of-gastric-cancer
#7
Weiming Li, Huan Jia, Jichang Wang, Hao Guan, Yan Li, Dan Zhang, Yanan Tang, Thomas D Wang, Shaoying Lu
Early diagnosis and evaluation of prognosis are both crucial for preventing poor prognosis of patients with gastric cancer (GC), a leading cause of cancer-related deaths worldwide. Cluster of differentiation 44 (CD44), an indicator of cancer stem cells, can be specifically targeted by molecular probes and detected in tissues of GC in a large quantity. In current study we found that RP-1, a specific peptide binding to CD44 protein, exhibited the potentials of specific binding to CD44 high-expressing cancer cells both in vitro and in vivo, and the capacity of predicting prognosis of human GC in a microarray assay...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415621/usp22-maintains-gastric-cancer-stem-cell-stemness-and-promotes-gastric-cancer-progression-by-stabilizing-bmi1-protein
#8
Yue Ma, Hua-Lin Fu, Zhen Wang, Hai Huang, Jian Ni, Jie Song, Ying Xia, Wei-Lin Jin, Da-Xiang Cui
Increased ubiquitin-specific protease 22 (USP22) has been associated with poor prognosis in several cancers including gastric cancer. However, the role of USP22 in gastric tumorigenesis is still unclear. Gastric cancer stem cells have been identified and shown to correlate with gastric cancer initiation and metastasis. In this study, we found that silencing of USP22 inhibited proliferation of gastric cancer cells and suppressed the cancer stem cell spheroid formation in serum-free culture. Furthermore, cancer stem cell markers, such as CD133, SOX2, OCT4 and NANOG were down-regulated...
March 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28404967/gli1-mediated-regulation-of-side-population-is-responsible-for-drug-resistance-in-gastric-cancer
#9
Beiqin Yu, Dongsheng Gu, Xiaoli Zhang, Jianfang Li, Bingya Liu, Jingwu Xie
Gastric cancer is the third leading cause of cancer-related mortality worldwide. Chemotherapy is frequently used for gastric cancer treatment. Most patients with advanced gastric cancer eventually succumb to the disease despite some patients responded initially to chemotherapy. Thus, identifying molecular mechanisms responsible for cancer relapse following chemotherapy will help design new ways to treat gastric cancer. In this study, we revealed that the residual cancer cells following treatment with chemotherapeutic reagent cisplatin have elevated expression of hedgehog target genes GLI1, GLI2 and PTCH1, suggestive of hedgehog signaling activation...
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28403178/relationship-between-epithelial-cell-adhesion-molecule-epcam-overexpression-and-gastric-cancer-patients-a-systematic-review-and-meta-analysis
#10
REVIEW
Meng Dai, Fei Yuan, Cuiqun Fu, Guodong Shen, Shilian Hu, Gan Shen
OBJECTIVES: The epithelial cell adhesion molecule (EpCAM) is one of the most commonly used markers of cancer stem cells (CSCs), but the clinical and prognostic significance of EpCAM in gastric cancer (GC) remains disputable. Motivated by heterogeneous and inconclusive results, we conducted a systematic review and meta-analysis to systematically summarize and elucidate the association between EpCAM overexpression and GC patients. METHODS: The PubMed, Cochrane Library, Medline, Web of Knowledge and the China National Knowledge Infrastructure (CNKI) databases were searched to identify relevant studies...
2017: PloS One
https://www.readbyqxmd.com/read/28398518/epigenetic-regulation-of-cd133-in-gastrointestinal-stromal-tumors
#11
Helene Geddert, Alexander Braun, Claudia Kayser, Arno Dimmler, Gerhard Faller, Abbas Agaimy, Florian Haller, Evgeny A Moskalev
Objectives: This study ascertained the regulation of the stem cell marker CD133 and its potential applicability for prognostication of gastrointestinal stromal tumors (GISTs). Methods: A total of 95 resected GISTs were included in the study. CD133 protein expression was assessed immunohistochemically on tissue microarrays. Methylation percentage was quantified by pyrosequencing. Gene expression in cell lines GIST48b and GIST882 upon treatment with DNA demethylation agent 5-aza-2'-deoxycytidine was analyzed by quantitative polymerase chain reaction...
April 7, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28393208/isolation-of-cancer-progenitor-cells-from-cancer-stem-cells-in-gastric-cancer
#12
Zhiyang Shang, Yingxin Xu, Wentao Liang, Kai Liang, Xiang Hu, Lei Wang, Zhenyu Zou, Yue Ma
The success of cancer treatment may depend on the complete elimination of cancer stem cells (CSCs). However, data regarding the current characterization of CSCs in different types of tumor are inconsistent, possibly due to the mixture of CSCs with cancer progenitor cells (CPCs). Therefore, it is important to exclude CPCs for the characterization of CSCs. The present study aimed to characterize gastric cancer stem cells (GCSC) by separating GCPC from gastric progenitor cells (GCSC) with flow cytometry. In total, 615 murine gastric cancer (GC) cells were divided into aldehyde dehydrogenase (ALDH)high, ALDHlow and ALDHneg groups by flow cytometry according to their ALDH activity...
April 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28383763/upregulated-mir-132-in-lgr5-gastric-cancer-stem-cell-like-cells-contributes-to-cisplatin-resistance-via-sirt1-creb-abcg2-signaling-pathway
#13
Lanfang Zhang, Xiaohe Guo, Dezhong Zhang, Yingying Fan, Lei Qin, Shuping Dong, Lanfang Zhang
Cisplatin resistance has long been a major problem that restricts its use. A novel paradigm in tumor biology suggests that gastric tumor chemo-resistance is driven by gastric cancer stem cell-like (GCSCs). Growing evidence has indicated that microRNAs (miRNAs) contributes to chemo-resistance in gastric cancer (GC). Here, Lgr5(+) cells derived from gastric cancer cell lines displayed stem cell-like features. Flow cytometry demonstrated the presence of a variable fraction of Lgr5 in 19 out of 20 GC specimens...
April 6, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28378630/the-self-renewal-signaling-pathways-utilized-by-gastric-cancer-stem-cells
#14
REVIEW
Ying Fu, Hui Li, Xishan Hao
Gastric cancer is a leading cause of cancer-related mortality worldwide. Cancer stem cells are the source of tumor recurrence and metastasis. Self-renewal is a marker of cancer stem cells and also the basis of long-lasting survival and tumor progression. Although the mechanism of gastric cancer stem cell self-renewal is not clear, there are several signaling pathways and environmental factors known to be involved. This mini review describes recent developments in the self-renewal signaling pathway of gastric cancer stem cell research...
April 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28377511/unsaturated-fatty-acyl-recognition-by-frizzled-receptors-mediates-dimerization-upon-wnt-ligand-binding
#15
Aaron H Nile, Susmith Mukund, Karen Stanger, Weiru Wang, Rami N Hannoush
Frizzled (FZD) receptors mediate Wnt signaling in diverse processes ranging from bone growth to stem cell activity. Moreover, high FZD receptor expression at the cell surface contributes to overactive Wnt signaling in subsets of pancreatic, ovarian, gastric, and colorectal tumors. Despite the progress in biochemical understanding of Wnt-FZD receptor interactions, the molecular basis for recognition of Wnt cis-unsaturated fatty acyl groups by the cysteine-rich domain (CRD) of FZD receptors remains elusive. Here, we determined a crystal structure of human FZD7 CRD unexpectedly bound to a 24-carbon fatty acid...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28361321/formation-of-stomach-tissue-by-organoid-culture-using-mouse-embryonic-stem-cells
#16
Taka-Aki K Noguchi, Akira Kurisaki
In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28361317/generation-of-gastrointestinal-organoids-from-human-pluripotent-stem-cells
#17
Jorge O Múnera, James M Wells
Over the past several decades, developmental biologists have discovered fundamental mechanisms by which organs form in developing embryos. With this information it is now possible to generate human "organoids" by the stepwise differentiation of human pluripotent stem cells using a process that recapitulates organ development. For the gastrointestinal tract, one of the first key steps is the formation of definitive endoderm and mesoderm, a process that relies on the TGFb molecule Nodal. Endoderm is then patterned along the anterior-posterior axis, with anterior endoderm forming the foregut and posterior endoderm forming the mid and hindgut...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28356974/gastrointestinal-stromal-tumor-with-a-pdgfra-mutation-masquerading-as-gastric-plexiform-fibromyxoma-a-comparative-clinicopathological-study-of-two-cases
#18
Jun Zhou, Jingjing Xu, Guozhong Jiang, Yihui Ma, Jingwen Qi, Wencai Li, Dandan Zhang
Gastric plexiform fibromyxoma (PF) is a rare mesenchymal tumor with a histologically distinctive multinodular pattern, dissimilar to conventional gastrointestinal stromal tumor (GIST). The current study presents one case of gastric PF, and one case of GIST with a platelet-derived growth factor receptor α (PDGFRA) mutation mimicking PF, and discusses their differential diagnoses. The two patients were a 51-year-old male with PF and a 47-year-old female with GIST, each of whom presented with an occupying lesion in the gastric antrum...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28356964/prognostic-significance-of-klf4-expression-in-gastric-cancer
#19
Isaya Hashimoto, Takuya Nagata, Shinichi Sekine, Makoto Moriyama, Kazuto Shibuya, Shozo Hojo, Koshi Matsui, Isaku Yoshioka, Tomoyuki Okumura, Takashi Hori, Yutaka Shimada, Kazuhiro Tsukada
To understand the roles of pluripotent stem cell-inducing genes in gastric cancer, the expression of Krüppel-like factor 4 (KLF4), Nanog, octamer-binding transcription factor 4 (Oct4), avian myelocytomatosis viral oncogene homolog (c-Myc) and sex-determining region Y-box 2 (SOX2) was examined using the newly developed gastric carcinoma tissue microarray. The associations between the immunohistochemical expression levels of the pluripotency-inducing factors and the clinicopathological data of 108 patients with gastric cancer were analyzed...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28352350/expression-of-nuak2-in-gastric-cancer-tissue-and-its-effects-on-the-proliferation-of-gastric-cancer-cells
#20
Lin Tang, Shu-Juan Tong, Zhen Zhan, Qian Wang, Yuan Tian, Feng Chen
The present study aimed to analyze the expression and effects of NUAK2 in gastric cancer and adjacent normal gastric tissues. The protein expression levels of NUAK2 were detected by western blot analysis. The effects of NUAK2 expression on the proliferation of gastric cancer cells was detected using an MTT and BrdU incorporation assay. Furthermore, the effects of NUAK2 on proliferation and cancer stem cell markers, both protein and microRNA (miRNA), were investigated by western blot analysis and miRNA microarrays, respectively...
February 2017: Experimental and Therapeutic Medicine
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